Soot combustion experiments with 5%O2/He were conducted using model soot, and four distinct compositions of CexPr1-xO2-δ oxides were prepared, varying nominal cerium com-positions (x=0, 0.2, 0.3, and 1). The catalyst samples were comprehensively characterized using techniques such as XRD, Raman spectroscopy, HR-TEM, N2 adsorption at −196 °C, XPS, O2-TPD, H2-TPR, and work function measurements. The Pr-rich compositions, rang-ing from Ce0.3Pr0.7O2-δ to PrO2-δ, resulted in a significant increase in the total evolved O2 amounts and enhanced catalyst reducibility. However, a decrease in textural properties of the catalysts was noted, being particularly important for the pure praseodymia under the synthesis route conducted. The catalytic activity was investigated under two contact modes of mixing between soot and catalyst: loose and tight. The results revealed that the catalytic performance is associated with the surface contact in tight contact mode and with the combination of surface/subsurface/bulk oxygen mobility and the BET surface ar-ea in loose contact mode. Notably, the T10 and T50 parameters were achieved at much lower temperatures than the uncatalyzed soot combustion, even under loose contact conditions. Specifically, the 50% conversion was achieved at 511℃ and 538℃ for Ce0.3Pr0.7O2 and Ce0.2Pr0.8O2, respectively. While no direct correlation between catalytic activity and work function was observed a significant relationship emerges between work function values and the formation of oxy-gen vacancies, whatever the conditions used for these measurements. On the other hand, the ability to generate a high population of oxygen vacancies at low temperatures, rather than the direct activation of gas-phase O2, influences the catalytic performance of Pr-doped ceria catalysts, highlighting the importance of surface/subsurface oxygen vacancy generation, which was the parameter which showed better correlation with the catalytic activity, whatever the soot conversion value or the mode of contact considered.

The paper provides a discussion on the results of studies of the effect exerted by combined degradation factors typical of four types of wear: abrasion, impact-abrasion, tribocorrosion, and impact-abrasion-corrosion, conducted for chain wheels made of Ni-Cu alloyed Austempered Ductile Iron. The studies consisted in determining the content of retained austenite in the structure of the cast irons in question, establishing the measures of wear following wear testing, and identifying basic surface degradation mechanisms observed in the chain wheels tested following multifactorial wear processes. The chain wheels made of ADI were found to have sustained the greatest damage under the impact-abrasion-corrosion (three-factor) wear scenario, while the wear was least advanced in the abrasion (one-factor) wear case. Another observation derived from the studies is that the combined effect of dynamic forces, corrosion, and quartz sand-based abrasives causes increased surface degradation in the cast iron grades taken into consideration compared to processes characterised by a reduced number of degradation factors (i.e. one- or two-factor wear processes). Additional hardness tests and XRD analyses have revealed a distinctive effect attributable to combined degradation factors on the surface hardness increase value and implied that bench testing was followed by phase transition.

Smart home and building systems are popular solution that support maintaining comfort, safety and improving energy efficiency in buildings. However, dynamically developing distributed network technologies, in particular the Internet of Things, are increasingly entering the above-mentioned application areas of building automation, offering new functional possibilities. The result of these processes is the emergence of many different solutions that mix technologies of field-level and ICT networks in various configurations and architectures. New paradigms are also emerging, such as edge and fog computing, providing support for local monitoring and control networks in the implementation of advanced functions and algorithms, including machine learning and artificial intelligence mechanisms. This paper collects state-of-the-art information in these areas, along with a systematic review of the literature, case-studies and an analysis of selected development trends. The author systematized this information in the context of the potential development of building automation systems. Based on the conclusions of this analysis and discussion, a framework for the development of the Generic IoT paradigm in smart home and building applications has been proposed, along with a SWOT analysis of its usability. Future works are proposed as well.

Although ladybirds of European Russia and the Caucasus have been the subject of numerous ecological and faunistic investigations, there is an evident lack of appropriate identification key for them. All previous keys have been published in Russian. The most modern key was published more than 50 years ago and included only about 60 % of species. Guides for identification of Coccinellidae of other countries are not appropriate for European Russia, since do not include many species occurring in the regions. New, original key to subfamilies, genera, and species of ladybirds (Coccinellidae) of the European Russia and Russian Caucasus is presented. All native species recorded in the region and all alien species introduced to this region are included. Some species from the adjacent regions are added. In total, 110 species are keyed and illustrated with line drawings. Photographs of rare and endemic species are provided. Information on the distribution of species within the region under consideration is provided. Synonymy of Chilocorus kuwanae with Ch. renipustulatus is presented and discussed.

In the scientific community, it is difficult to find a consensus on defining BIM. Just as the acronym BIM is developed in different ways, it is also understood in different ways. Depending on its understanding, different definitions emerge. It is defined differently by organizations and standards, still differently by academics. Many years of academic discourse on the subject have failed to produce a solution. Despite the fact that the acronym BIM has already done its work for the construction industry, it still stirs up excitement. There is still no clear definition, as the view of BIM varies from one perspective to another. This article attempts to sort out the definitions cited so far by important organizations and key academics. The review was based on deep literature study has attempted to be inclusive and consistent. The question still remains open: do we need a single, correct definition of BIM? The aim of the article is to try to answer this question, open up a renewed discussion and come to a satisfactory consensus. BIM can be identified with an activity, product or system. The article breaks down the definitions of BIM, identifies six key attributes of BIM, presents the evolution of the understanding of BIM and proposes new definitions in a narrow and broad approach.

Fluctuations of protein three-dimensional structures and large-scale conformational transitions are crucial for the biological function of proteins and their complexes. Experimental studies of such phenomena remain very challenging and therefore molecular modeling can be a good alternative or a valuable supporting tool for the investigation of large molecular systems and long-time events. In this mini-review, we present two alternative approaches to the coarse-grained (CG) modeling of dynamic properties of protein systems. We discuss two CG representations of polypeptide chains used for Monte Carlo dynamics simulations of protein local dynamics and conformational transitions and, on other hand, highly simplified structure-based Elastic Network Models of protein flexibility. In contrast to classical Molecular Dynamics the modeling strategies discussed here allow quite accurate modeling of much larger systems and longer time dynamic phenomena. We briefly describe the main features of these models and outline some of their applications, including modeling of near-native structure fluctuations, sampling of large regions of the protein conformational space, or possible support for the structure prediction of large proteins and their complexes.

It has recently been discovered that mere cell contact by human cytomegalovirus (CMV) particles leads to profound modulation of cellular gene expression. Reduced monocyte human leukocyte antigen (HLA-DR) expression is a novel biomarker of severity and outcome in many diseases. Modulation of CD14 protein by CMV was shown in vitro, but little is known about the phenomenon in vivo (during active cytomegalovirus disease). Therefore, we investigated monocyte CD14 and HLA-DR expression in CMV infected patients in relation to logarithmic phase of infectious process. Samples from patients with active CMV replication (exponential growth of CMV viremia) were tested. After CD45/SSC gating monocyte CD14 and HLA-DR expression were determined by double-color flow-cytometry. Significant monocytosis and poor correlation between CMV replication and CD14+HLA-DR(-) cells prompted CD14 investigation. During logarithmic phase of CMV infection increased count and percentage of CD14low monocytes were observed which correlated with viral replication in several clinical situations except when there was a rapid recovery without relapse. Furthermore, most of CD14low monocytes are HLA-DR+. The increase of CD14low monocytes is also observed under the influence of high dose of glucocorticoids (20 mg of dexamethasone). The reduction in CD14 induced by CMV and dexamethasone indicates that the monocyte balance is disturbed between the classical and non-classical phenotype. A high percentage of CD14lowHLA-DR+ probably gives rise to adaptive and a decrease of innate immune response. In light of the logarithmic increase of viral load (with exponent between 3,23 and 5,77), high monocytosis above 1200 / µl is a hallmark of CMV replication.

The nature of the interaction between Th17 cells and the blood-brain barrier (BBB) is critical for the development of autoimmune inflammation in the central nervous system (CNS). TNF-a or IL-17 stimulation is known to enhance the adherence of Th17 cells to the brain endothelium. The brain endothelial cells (bEnd.3) express VCAM-1, the receptor responsible for inflammatory cell adhesion, which binds VLA-4 on migrating effector lymphocytes at the early stage of brain inflammation. The present study examines the effect of the pro-inflammatory cytokines TNF-a and IL-17 on the adherence of Th17 cells to bEnd.3 The bEnd.3 cells were found to increase production of CCL2 and CXCL1 after stimulation by pro-inflammatory cytokines, while CCL2, CCL5, CCL20 and IL17 induced Th17 cell migration through a bEnd.3 monolayer. This interaction between Th17 cells and the brain endothelium appears to be mediated by VCAM-1 and some chemotactic cytokines. This observation may suggest potential therapeutic targets for the prevention of autoimmune neuroinflammation development in the CNS.

The size and distribution of abrasive particles have a significant influence on the effectiveness of the cutting process by the high-speed abrasive water jet (AWJ). The paper deal with the abrasive materials disintegration intensity in AWJ cutting during the creation of the abrasive jet. An evaluation of the abrasive materials grabbed after forming in the cutting head was carried out and its grain distribution was evaluated. Used here the arithmetic, geometric and logarithmic method of moments and Folk and Ward method. The influence of abrasive concentration of abrasive materials as alluvial garnet, recycled garnet, corundum, and olivine on grain distribution was studied. A recovery analysis was also carried out and the recycling coefficient for each tested abrasive material was determined.

This paper presents a method to determine material constants of a standard basketball shell together with the development of a virtual numerical model of a basketball. Material constants were used as the basis for the hyperelastic material model in the strain energy function (SEF). Material properties were determined experimentally by strength testing in uniaxial tensile tests. Additionally, the digital image correlation technique (DIC) was applied to measure strain in axial planar specimens, thus providing input stress-strain data for the Autodesk software. Analysis of testing results facilitated the construction of a hyperelastic material model. The optimal Ogden material model was selected. Two computer programmes by Autodesk were used to construct the geometry of the virtual basketball model and to conduct simulation experiments. Geometry was designed using Autodesk Inventor Professional 2017, while Autodesk Simulation Mechanical 2017 was applied in simulation experiments. Simulation calculations of the model basketball bounce values were verified according to the FIBA recommendations. These recommendations refer to the conditions and parameters which should be met by an actual basketball. A comparison of experimental testing and digital calculation results provided insight into the application of numerical calculations, designing of structural and material solutions for sports floors.

The article contains the results of experimental studies for a multi-opening orifice with substitute constriction factor of β = 0.5 (m = 0.25), mounted in a DN50 hydraulic measuring flume. Flow measurements were taken for a progressing turbulent flow within Reynolds numbers (Re = 4700...19500). Based on experimental data, flow characteristics as well as discharge coefficient C characteristics were determined. Relative expanded uncertainty of determining a discharge coefficient C was estimated within the changes of volume flow qv from 0.35 dm3/s to 0.68 dm3/s. The value does not exceed 1.25% within the changes of Reynolds numbers 9800 ≤ Re ≤ 19500.

It is not understood how the numbers and identities of vertebrae are controlled during mammalian development. The remarkable robustness and conservation of segmental numbers may suggest a digital nature of the underlying process. Here I propose a mechanism that allows cells to obtain and store the segmental information in digital form, and to produce a pattern of chromatin accessibility that in turn regulates Hox gene expression specific to the metameric segment. The model requires that a regulatory element be present such that the number of occurrences of the motif between two consecutive Hox genes equals the number of segments under the control of the anterior gene. This is true for the recently discovered HRC3 motif, associated with histone modifications and developmental genes. The finding not only allows correctly predicting the numbers of segments using only sequence information, but also resolves the 40-year-old enigma of the function of temporal and spatial collinearity of Hox genes. The logic of the mechanism is illustrated in an animated video: https://youtu.be/4a3XOQ7Lz28. I also discuss how different aspects of the proposed mechanism can be tested experimentally.

Accurate geometric modeling of blood vessels lumen given their 3D images is a basis for vessel quantification in support of cardiovascular diseases diagnosis, treatment and monitoring. Unlike other approaches, which assume circular or elliptical vessel cross-section, the proposed method employs parametric B-splines combined with the image formation system equations to accurately localize the highly curved lumen boundaries. No image segmentation is involved which might reduce the localization accuracy in effect of spatial discretization. It is shown that the model parameters can be reliably identified by a feedforward neural network which, driven by the cross-section images, predicts the parameter values many times faster than a reference least-squares (LS) model fitting algorithm. Two example applications: modeling the lower extremities artery-vein complexes visualized in steady-state contrast-enhanced magnetic resonance images (MRI) and the coronary arteries pictured in computed tomography angiograms (CTA), are presented and discussed to demonstrate the method potential. Besides application to medical diagnosis, blood-flow simulation and vessel-phantom design, it can serve as a tool for automated annotation of image datasets required to train machine-learning algorithms.

Although lymphoma is the most frequent malignancy in common variable immunodeficiency (CVID), solid tumors, especially under influence of oncogenic viruses, are not considered. Furthermore, in vitro genetic studies and cell cultures are not adequate for immune system and HBV interaction. We adopted previously introduced clinical model of host-virus interaction (i.e. infectious process in immunodeficiency) for analysis of B cells and specific IgG role (observational study of a CVID patient who received intravenous immunoglobulin (IVIG)) Suddenly, the patient deteriorated with positive result of HBs, HBV-DNA (369x106copies). In spite of lamivudine therapy and IVIG escalation (from 0.3 to 0.4g/kg), CT showed 11cm intrahepatic tumor (hepatocellular carcinoma). Anti-HBs had been positive in time-lapse analysis (range 111-220 IU/ml). Replacement therapy intensification was complicated by immune complex disease with renal failure. The fulminant HCC in CVID and the development of the tumor as the first sign is interesting. Unfortunately, treatment with hepatitis B immune globulins (HBIG) plays a major role in posttransplant maintenance therapy. Anti-HBs substitution has not been proven to be effective, oncoprotective nor safe. Therefore, immunosuppression in HBV-infected recipients should be carefully minimized, patient selection more precise with the exclusion of HBV-positive donors. Our clinical model show HCC pathway with important humoral host factors, contrary to epidemiological/cohort studies highlighting risk factors only (e.g. chronic hepatitis). Lack of cell cooperation, B cell deficiency observed in CVID plays a crucial role in high HBV replication, especially in carcinogenesis.

This study presents a novel approach for predicting hierarchical short time series. In this article, our objective was to formulate long-term forecasts for household natural gas consumption by considering the hierarchical structure of territorial units within a country's administrative divisions. For this purpose, we utilized natural gas consumption data from Poland. The length of the time series was an important determinant of the data set. We contrast global techniques, which employ a uniform method across all time series, with local methods that fit a distinct method for each time series. Furthermore, we compare the conventional statistical approach with a machine learning (ML) approach. Based on our analyses, we devised forecasting methods for short time series that exhibit exceptional performance. We have demonstrated that global models provide better forecasts than local models. Among ML models, neural networks yielded the best results, with the MLP network achieving comparable performance to the LSTM network while requiring significantly less computational time.

The paper presents a model of moisture transport in wood taking into account diffusion and the accompanying adsorption of water vapour through the skeleton. A two-parameter form of the source term was proposed, depending on the distance of the current mass concentration of bound water from the equilibrium state. The tests on cubic samples with a side of 2 cm were carried out which allowed to determine the coefficients of the proposed model on the basis of the reverse method. The tests were performed for pine, larch, oak and ash in all directions of orthotropy. Also the tests on thin samples were performed to verify the source term.

The article presents an induction generator connected to the power grid using the AC / DC / AC converter and LCL coupling filter. In the converter, both from the generator side and from the power grid side, three-level inverters were used. The algorithm realizing Pulse Width Modulation (PWM) in inverters has been simplified to the maximum. Control of the induction generator was based on the Direct Field Oriented Control (DFOC) method. At the same time, voltage control has been used for this solution. The MPPT algorithm has been extended to include the variable pitch range of the wind turbine blades. The active voltage balancing circuit has been used in the inverter DC voltage circuit. In the control system of the grid converter with an LCL filter, the number of measurements was limited to the measurement of power grid currents and voltages. Synchronization of control from the power grid side is ensured by the use of a PLL loop with the system of preliminary suppression of undesired harmonics (CDSC).

Background: Humoral memory and specific antibodies levels depend on kind of the antigen and individual immunofactors., The presence of IgM antibodies or fourfold rise in specific IgG level, are generally accepted as diagnostic in serology of acute viral infection. This basic model is not adequate for herpesvirome especially after hematopoietic stem cell transplantation (HSCT) due to continuous, usually multifocal antigenic stimulation, various donor serostatus, immunosuppresion and individual immunoreconstitution. Methods: A  case-control study was conducted for identifying cases of Human herpesviruses (HHV) active infection (from 300 diagnosed immunocompromised patients) and evaluating historically associated humoral factors to look at outcomes. We enrolled only patients’ data with meticulous differential diagnosis to exclude other causes, thereby observe pathways and temporal relationships, not statistical ones, usually collected in cohorts. Despite the small number such data collection and analysis avoids a number of biases and indicates cause and effect.Results. In the observational study retrospective analysis of data from 300 patients with clinical diagnosis of herpes simplex (HSV) and varicella zoster virus(VZV) reactivation showed a number of biases. In two well differentiated cases (confirmed by Tzanck test) with various disease and conditioning evolution of immunoparameters showed interesting pathway. Exponential decrease of specific IgG after HSCT precedes virus replication with cytopathic effect (shingles, VZV-encephalitis and HSV-induced mucositis). Minima (smallest IgG levels) before herpes virus reactivation were 234,23 mIU/ml and 94 RU/ml for VZV and HSV, respectively. This coincides with low CD4 titer, but without other infectious process. Other immune response parameters such as Treg, cytotoxic T cells, complement and total IgG level were quite the same as before the transplant procedure. Interestingly, the second wave of immunoreconstitution with anamnestic antibody response was not always observed. It coincided with prolonged herpes viral infection. Patient with lymphocyte depletion in conditioning showed earlier second wave of immunoreconstitution (6th vs 14th month) Conclusions: Typical for infancy kinetics of IgG level is unique after HSCT (the decline phase is first). Host-microbiome factors (i.e. HHV1-3 -serostatus) should be taken into account to predict risk of non-relapse mortality and survival after HSCT. The levels of specific antibodies help to predict the prognosis and improve the management. The lack of differentiation, assessor and confusing bias are the main obstacles in statistical HHV1-3 research. Such time-lapse case studies may be first to build evidence of a pathway and association between immunoparameters and HHV disease.

Poland is the 3^rdproducer of mushrooms in the world. Mushroom production in Poland accounts for nearly 25% of the total production in the EU, and it is still growing. One type of waste generated during mushroom production is mushroom spent compost (MSC), with a 5:1 (MSC: mushrooms) production rate. We investigated valorizing the MSC to produce fuel via torrefaction (‘roasting’, a.k.a. low-temperature pyrolysis). Specifically, we developed models for the MSC torrefaction kinetics using thermogravimetric analyses (TGA) and the effects of torrefaction temperature (200~300 °C) and process duration time (20~60 min) on the resulting biochar (fuel) properties. The estimated activation energy value of MSC torrefaction was 22.3 kJ^.mol^-1. The highest higher heating value(HHV) = 17.9 MJ^.kg^-1d.m. was found for 280 °C (60 min torrefaction time). The temperature of torrefaction significantly (p<0.05) increased the HHVfor constant process duration. The torrefaction duration time significantly (p<0.05) increased the HHVfor 220 °C and decreased HHVfor 300 °C. The highest mass yield 98.5% was found for 220 °C (60 min), while the highest energy yield was found for 280 °C (60 min). In addition, estimations of the value (€132.3·Mg^-1d.m. or 27.7 €·Mg^-1w.m) and quantity of resulting biochar (from torrefied MSC with 65.3% moisture content) were made based on the 280°C (60 min) torrefaction variant, assuming the price of commercially available coal fuel. We have shown a concept for an alternative utilization of abundant biowaste (MSC). The initial economic evaluation showed that MSC torrefaction might be profitable. This research provides a basis for alternative use of an abundant biowaste and can help charting improved, sustainable mushroom production.

Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

The objectives of this article are to examine whether the application of digitalization in enterprise budgeting affects the fulfilment of budgeting functions and their qualitative characteristics, and to investigate whether the realization of budgeting functions brings benefits. The research was conducted from the perspective of contingency theory, both internal and external conditions. The study used an online survey targeting company budgeting staff. Structural Equation Modelling was utilized to validate the structural model (PLS-SEM technique). The conclusions confirm that the application of digitalization, which particularly pertains to ERP, Business Intelligence systems and accounting systems with budgeting solutions, positively impacts the fulfilment of budgeting functions and most of their qualitative characteristics, while Blockchain makes a minor contribution. Our research suggests that contingency variables, particularly internal ones such as company size, the quality of the organizational structure, and support for competitive strategy, continue to affect budgeting implementation even with the participation of digitalization in budgeting. Budgeting functions and their qualitative aspects are notably correlated with the advantages derived from budgeting, including financial performance, sustainable development and budget task execution.

Using the projection evolution (PEv) approach, time can be included in the quantum mechanics as an observable. Having the time operator, it is possible to explore the temporal structure of various quantum events. In the present paper we discuss the possibility of constructing a quantum clock, which advances in time during its quantum evolution, in each step having some probability to localize itself on the time axis in the new position. We propose a working two-state model as the simplest example of such a clock.

The study shows that the volumes and surfaces of $n$-balls, $n$-simplices, and $n$-orthoplices are holomorphic functions of $n$, which makes those objects omnidimensional, that is well defined in any complex dimension. Applications of these formulas to the omnidimensional polytopes inscribed in and circumscribed about $n$-balls reveal previously unknown properties of these geometric objects. In particular, for $0 < n < 1$, the volumes of the omnidimensional polytopes are larger than those of circumscribing $n$-balls, and both their volumes and surfaces are smaller than those of inscribed $n$-balls. The surface of an $n$-simplex circumscribing unit diameter $n$-ball is spirally convergent to zero with real $n$ approaching negative infinity but first has a local maximum at $n=-3.5$. The surface of an $n$-orthoplex circumscribing unit diameter $n$-ball is spirally divergent with real $n$ approaching negative infinity but first has a local minimum at $n=-1.5$, where its real and imaginary parts are equal to each other; similarly, as its volume, where the similar local minimum occurs at $n=-3.5$. Reflection functions for volumes and surfaces of these polytopes inscribed in and circumscribed about $n$-balls are proposed. symmetries of products and quotients of volumes in complex dimensions $n$ and $-n$ and of surfaces in complex dimensions $n$ and $2-n$ are shown to be independent of the metric factor and the gamma function. Specific symmetries also hold between volumes and surfaces in dimensions $n = -1/2$ and $n = 1/2$.

According to a number of authors, current social-economic and environmental problems require radical solutions, including the change of our approach to economy and looking for new models of its functioning. In Poland such trends are being recognised as well, having their reflection in the economic life. An approach described in this article concerns an intermediate sharing model, which may be placed between the most archetypical sharing, which is applied, among other, in family relationships, and sharing platforms that are raising growing controversies, such as UBER and Airbnb. The article uses the analysis of solutions applied in this regard in Poland and a survey carried out among students of the Faculty of Economics, University of Gdańsk, aimed at determining factors that affect changes in consumer behaviour.

The article discusses the issue of the sustainable development of the cross-border market for cultural services in a city divided by the state border. The article uses the example of Cieszyn and Český Těšín, a city divided following the decision of the Council of Ambassadors in 1920. The research carried out so far indicates the main constraints in the harmonious functioning of the cross-border market for cultural services in this city, such as: different cultural policies implemented on both sides of the city, language barriers as well as legal and administrative differences. Therefore, the authors undertook research aimed at recognising the role of Euroregional structures in stimulating the sustainable development of this region. On the basis of the analysis of the Cieszyn Silesia Euroregion's documentation and the results of qualitative and quantitative research, the article describes the role of the Euroregion in building a cross-border market for cultural services. Recommendations were also prepared that could constitute the principles of a common cultural policy not only for Cieszyn and Český Těšín, but also for other European cities in the Schengen Area, which, like Cieszyn and Český Těšín, have been divided by a state border.

Energy from biogas is one of the most promising renewable energy sources. Significant, for technical, economic, and environmental reasons, is the construction of agricultural microgas plants next to households specialising in cattle rearing. The availability of free substrate makes it possible not only to produce one's electricity and heat but also to produce a more environmentally and human-friendly manure. The operation of biogas plants is, unfortunately, associated with technical problems. This paper presents the results of a study of the quality of electricity generated in an agricultural biogas plant built at a farm specialising in dairy cows, where the owner reported a problem with the voltage in the power supply network. Therefore, selected power quality parameters occurring at the connection point of the biogas plant were analysed in detail. As the authors' research showed, in the case in question, the agricultural biogas plant was connected via a cable with too small a conductor cross-section, which resulted in significant voltage exceedances, making it impossible to switch on the second of the biogas plant's generators. Replacement of the feed-in cable has enabled both generators to run, but significant fluctuations in the feed-in voltage values were still noticeable. Analysis of the subsequent measurements revealed the need for changes in the design of the digester itself. The proposal for change is to replace the two current digester mixers (each rated at 9 kW) with more mixers of lower power. The new mixers would operate in an alternating system, where only one would always be activated.

The correct classification of defects originating from partial discharges (PD) in medium-voltage (MV) switchgears with air insulation (AIS) remains a challenging research topic for scientists worldwide. In this article, the authors simulated four possible defects occurring in the power industry, including one that is a simultaneous combination of two commonly ones. In addition, the correctness of the algorithm was checked by adding a classification class without any fault. The measurement signals were recorded with TEV sensors. The effectiveness of various hy-brid-connected neural networks was tested and discussed: GoogleNet and SqueezeNet based on spectrograms, SAE with FNN, 2D-CNN with LSTM, and hybrid AE combined with CNN and LSTM. The highest effectiveness – approximately 97% – was demonstrated by the GoogleNet and SqueezeNet networks. The research results are expected to form the basis for the development of a universal and wireless capacitive sensor for monitoring the level of PD in switchgears.

This work shows a correlation between light reflectance, absorption, and morphologies of series of (1-x) BM–x PT, (x = 0.0, 0.02, 0.04, 0.08, 0.12, 0.16, 0.24) ceramics composite. The (1-x) BM–x PT showed features of a black mirror with a low optical energy gap. The measured Vis-NIR diffused reflectance enabled the calculation of the energy gap using the modified Kubelka-Munk function. The estimated energy gap was lower than 1.5 eV related to low reflectance in the Vis-NIR range. Moreover, obtained histograms of grains, using scanning electron microscope, enabled the correlation between grains size and amount of lead titanate. We deduced from the ceramics surface morphology that marked porosity also induced reflectivity of low magnitude. We correlated the magnitude of the energy gap with phases of the BM-PT composite and with the electrical conductivity activation energy reported in the literature. Our results findings opened prospect studied materials for optical applications.

Monte Carlo Filtering (MCF) is one of the methods of Experimental Statistical Energy Analysis (E-SEA), which allows the correction of a negative LF (Loss Factor). In this article, a modification of the MCF method, called DESA (Diagonal Extension of the Search Area), is proposed. The technique applies a non-uniform extension of the search area when generating a population of normalized energy matrices. The degree of expansion of the search area is controlled by the Diagonal Penalty Factor (DPF). The authors demonstrated the method's effectiveness on a system that could not be identified in several frequency bands by the classical MCF method. After applying DESA, it was possible to fill in the problematic bands that were missing CLF and DLF values. The paper also proposes a way to minimize the errors introduced by using overly high DPF values.

The new climate law introduces a policy of sustainable construction, the assumption of which is the reduction of CO2 by the construction industry and the use of environmentally friendly materials, such as agricultural, mineral, and recycled waste, while limiting the consumption of natural resources. The article is a literature review that analyzes selected waste materials from various sectors of the economy that can be used as additives or partial substitutes for natural resources in the production of cement and in and cement building materials, the production of which reduces CO2 emissions, producing materials with high mechanical strength and environmentally friendly.

AlphaFold is a groundbreaking Deep Learning tool for protein structure prediction. It achieved remarkable accuracy in modeling the many 3D structures while taking as the input only the known amino acid sequence of proteins in question. Intriguingly though, in the early steps of each individual structure prediction procedure, AlphaFold is not respecting topological barriers that in real proteins result from the reciprocals impermeability of polypeptide chains. This study aims to investigate how this non-respecting of topological barriers affects AlphaFold predictions with respect to the topology of protein chains. We focus on such classes of proteins that during their natural folding form reproducibly the same knot type on their linear polypeptide chain as revealed by their crystallographic analysis. We use partially artificial test constructs in which the mutual non-permeability of polypeptide chains should not permit the formation of such knots during natural protein folding. We find that despite the formal impossibility that the protein folding process could produce such knots, AlphaFold predicts these proteins to be knotted. Our study underscores the necessity for cautious interpretation and further validation of topological features in protein structures predicted by AlphaFold.

Background. Intravenous iron therapy is used in treatment of anemia in uremic pa-tients. In patients treated with peritoneal dialysis iv. infused iron diffuses into the peritoneal cavity and may cause injury to the mesothelial cell. Methods. We studied effect of Iron Isomaltoside (IIS) in presence of NF-κβ inhibitor dehydroxymethyepoxyquinmicin (DHMEQ) on function of the peritoneal mesothelial cells. Experiments were performed on human peritoneal mesothelial cells in in vitro culture ex-posed to IIS 15 ug/dL ± DHMEQ 1 ug/mL. Intracellular oxidative stress, secretory activity and collagen synthesis in the cells were studied. Results. IIS induced oxidative stress in the mesothelial cells and that effect was weaker in presence of DHMEQ (-52%,p<0.001). In cells exposed to IIS increased expression of genes for IL6 (+74%,p<0.001), PAI-1 (+43%, p<0.01) and TGFβ (+53%,p<0.001) was observed and reduced for tPA (-36%,p<0.01). In presence of IIS increased secretion of IL6 (+56%, p<0.001), TGFβ (+49%,p<0.001) and PAI-1 (+51%, p<0.001) was observed whereas secretion of tPA was reduced (-25%, p0.001). DHMEQ reduced changes in genes and secre-tory activity caused by IIS. In presence of IIS synthesis of collagen in mesothelial cells was increased (+45%,p<0.001) and that effect was weaker (-25% vs. IIS, p<0001) when simulta-neously DHMEQ was used. Discussion. IIS induces proinflammatory changes in mesothelial cells, deteriorates their fibrinolytic activity and stimulates synthesis of collagen. All these effects are reduced when simultaneously NF-κβ inhibitor – DHMEQ is used.

According to some authors, the serum selenium level is strongly associated with the severity of liver diseases including liver cirrhosis. The aim of the study was to determine the relationship between the concentration of selenium and pro-inflammatory and profibrotic cytokines – interleukin-6 (IL-6) and growth differentiation factor 15 (GDF-15) in patients with alcoholic liver cirrhosis. The parameters studied were determined in serum of 99 alcoholic liver cirrhosis patients divided based on the severity of disease according to the Child-Turcotte-Pugh criteria. In patients with liver cirrhosis, the serum selenium concentration was statistically lower whereas serum IL-6 and GDF-15 concentrations were higher than those in the control group. Moreover, the concentration of selenium negatively correlated with the levels of GDF-15 and IL-6. The above results may indicate a role of selenium deficiency in the pathogenesis and progression of alcoholic liver disease.

The aim of the study was to verify whether a 20 and 40% reduction of nitrogen (N) fertilization rate will be sufficient to maintain soil basic chemical features, fertility, and yielding in monoculture maize cultivation in the no-tillage (NT) system versus the traditional plowing (PL) system. In addition, it was examined which tillage system (PL, NT) allows reduction of fertilization while maintaining good yields of the tested soils. Two fields (10 ha each) were established for PL and NT maize cultivation and soils (0-20 cm) were sampled two times per year - in spring (before maize sowing) and in autumn (after maize harvesting). A broad range of chemical and biological parameters (i.e. pH, forms of nitrogen, phosphorus and carbon, content of selected macronutrients and humic substances, respiration activity) were monitored during the experiment. It was evidenced that the 20% reduction in N fertilization does not have an adverse effect on the chemical and biological characteristics of soils. In turn, the maize yield was mainly the result of both the tillage system and the N rate, i.e. it was significantly higher in the PL system.

The aim of the study was to verify whether a reduced nitrogen (N) fertilization rate according to the EU recommendation (“From field to table” directive) may guarantee good yields and will be sufficient to maintain soil fertility in monoculture maize cultivation in the no-tillage (NT) system versus the traditional plowing (P) system. Two fields (10 ha each) were established for P and NT maize cultivation where the following N doses were applied: 0.0; 69.0, 92.0, and 115.0 kg ha-1. Soils (0-20 cm) were sampled two times per year - in spring (before maize sowing) and in autumn (after maize harvesting). A broad range of chemical (reaction, water holding capacity, total and easily degradable carbon, ammonium, nitrates, magnesium, calcium, potassium, sulfur) and biological (respiration, humic acids) parameters were monitored during the experiment. The vast majority of these factors had higher values in autumn than in spring. Although the role of reduced fertilization was not shown to be significant, the correlation analysis revealed some relationships between yield and nutrient availability. The 20% reduction in N fertilization does not have an adverse effect on the chemistry and biological characteristics of soils and does not worsen their chemical and biological quality.

(1) Background: The goal of the study was to evaluate psychological tolerance and health-related quality of life (QOL) in head and neck (HN) cancer patients treated with definitive accelerated radiotherapy (DART); (2) Methods: Seventy-six recurrence-free patients eligible for the study, who were treated with DART in the CAIR-2 phase III clinical study (median of follow-up=47 months), completed EORTC QLQ-C30 with H&amp;N35 module, Hospital Anxiety and Depression Scale (HADS) and Visual–Analog Scales (VAS) of pain in HN and the neck / arm area.; (3) Results: The most dominating symptoms measured with QLQ-C30 were: fatigue (44/100), sleeplessness (39/100), financial problems (38/100) and pain (32/100). Within H&amp;N35 the highest scores were reported on the subscales of sticky saliva (60/100), mouth dryness (65/100) and increased intake of painkillers (50/100). Pain (VAS) was reported by 87% (HN area) and 78% (shoulder) of the patients, with a mean score of 3/10. One-third reported depressive mood (HADS≥15 points) with an average score of 12.5/42 p. The depressed group, who smoked more as compared to non-depressed before DART (96% vs 78%) and required steroids treatment (85% vs 58%) during DART, also scored significantly worse on 23 of 35 subscales of QLQ-C30 and H&amp;N35 and experienced more intense pain (VAS). Women, and less advanced patients scored better in several aspects of the quality of life; (4) Conclusions: Patients treated with DART struggle with low quality of life and persisting treatment-related symptoms including constant pain. HNC survivors, especially those, who are depressed, may require additional psychosocial, rehabilitation and medical intervention programs..

A black hole in a Schwarzschild spacetime is considered. A transformation is proposed that describes the relationship between the coordinate systems exterior and interior to an event horizon. Application of this transformation permits considerations of the (a)symmetry of a range of phenomena taking place on both sides of the event horizon. The paper investigates two distinct problems of a uniformly accelerated particle. In one of these, although the equations of motion are the same in the regions on both sides, the solutions turn out to be very different. This manifests the differences of the properties of these two ranges.

Due to the increasing risk of extreme events caused by climate change (i.e. floods, fires, hurricanes) or wars, European veterinary public health may need some improvement. Utilizing a mix of qualitative (participatory observation) and quantitative methods (Internet mining), we analyzed the Greek media responses to the millennial flood in Thessaly (September 2023), focusing on animal health (including wild, companion animals and livestock) and public sentiment towards epizootic/epidemic threats. The study revealed a gap in crisis management plans regarding veterinary-related issues, emphasizing the need for comprehensive emergency response strategies. Our findings show how: (i) lay-referral system is projecting perception of epidemic threats into the population; (ii) emotional load of images of animal carcasses is misused by media creators aiming for big audience; (iii) pets owners are creating on-line communities for searching and treatment of their pets. Our results stress the importance of integrating crisis communication in consecutive phases of the discourse, such as (i) weather change; (ii) acute flood; (iii) recovery; (iv) outbreaks, into veterinary practices to better prepare for such disasters.

To date, only a few studies focused on carbon monoxide (CO) production during waste composting; all targeted on CO inside piles. Here, the CO net emissions from compost piles and the assessment of worker’s occupational risk of exposure to CO at large-scale composting plants are shown for the first time. CO net emissions were measured at two plants processing green waste, sewage sludge, or undersize fraction of municipal solid waste. Effects of the location of piles (hermetised hall vs. open yard) and turning (before vs. after) were studied. Higher CO net emission rates were observed from piles located in a closed hall. The average CO flux before turning was 23.25 and 0.60 mg‧m-2‧h-1 for hermetised and open piles, respectively, while after – 69.38 and 5.11 mg‧m-2‧h-1. The maximum CO net emissions occurred after the compost was turned (1.7x to 13.7x higher than before turning). The top sections of hermetised piles had greater CO emissions compared to sides. Additionally, 5% of measurement points of hermetised piles switched to ‘CO sinks’. The 1-h concentration in hermetised composting hall can reach max. ~50 mg CO∙m-3 before turning, and &gt;115 mg CO∙m-3 after, exceeding the WHO thresholds for a 1-h and 15-min exposures, respectively.

Wnt/β-catenin signaling pathway plays an established role in various diseases and is considered a hallmark of endometrial cancer (EC). Special AT-rich sequence-binding protein 1 and 2 (SATB1 and SATB2) are nuclear matrix-associated proteins involved in chromatin remodeling and regulation of gene expression. SATB1 promotes the progression of numerous types of cancers, whereas SATB2 acts as a tumor suppressor. Despite a recent progress in our knowledge about EC, the exact mechanisms that control their proliferation and metastatic potential still remain unknown. The aim of our study was to investigate the association between Wnt3A, β-catenin, SATB1 and SATB2 protein level and the clinicopathological features of EC patients. 92 EC patients, aged 37-84, were enrolled to our study. The immunoexpression of WNT3A was found in specimens from all EC patients, β-catenin was expressed in 97% of the cases, SATB1 in 87%. The significant association between Wnt3a expression and tumor grade was found; moreover mean IRS for Wnt3a turned out to be significantly lower in high-grade tumors than in low-grade malignancies (p=0.038). In turn, immunoexpression of β-catenin varied significantly across FIGO stages and was associated with the presence of lymph node metastases. Mean IRS for β-catenin in patients with lymph node metastases was significantly lower than in those without (p = 0.028). The Kaplan-Meier analyses demonstrated a stepwise impairment of cancer overall survival with increasing SATB1 expression. In conclusion, both Wnt/β-catenin signaling pathway and SATB1 contribute to progression of EC. Downregulation of β-catenin may predispose to lymphatic spread of EC. In turn, downregulation of Wnt3a seems to be characteristic for high-grade tumors, but probably does not play a role in formation of lymph node metastases. The important role of SATB1 as a predictor of poor survival and could be helpful in establishing a more accurate prognosis in endometrial cancer patients.

Low Emission Zone (LEZ) is an important mechanism to limit the negative environmental impact of traffic pollution in an urban area. Car traffic in the zone is strictly regulated to meet certain technical and environmental criteria. Such solutions of urban environment protection are gaining popularity, especially in European conurbations. Nowadays there are above 300 zones in Europe, mostly established in Italy and Germany. So far there are no LEZs in Eastern and Central European countries. However, the Warsaw authorities are just discussing launching of a LEZ in the city in 2024. According to the ongoing project, the zone is to be launched gradually, in five phases between 2024 and 2032, with the traffic criteria being tightened accordingly. The progressive emission reductions of the predominant pollutants in LEZ in consecutive phases were estimated on the basis of the Real Driving Emissions (RDE) test, carried out in 2020 by the ICCT. The gradually reduced emissions of NOx and PM were subsequently utilized as the input dataset for the regional CALPUFF model predictions, to calculate concentrations of these main traffic-induced pollutants within the LEZ area. The direct effect of the zone activation is reduction of air pollution in the urban center, which is mostly exposed to the pollution risk due to heavy traffic. Computer simulations reveal that implementation of the zone in the case of Warsaw will result in a quite significant reduction of NOx concentrations within the LEZ, such that its mean concentration in the zone becomes comparable to the rest of the conurbation. However, it will bring only a slight reduction of PM2.5 concentrations. This situation is due to the long-standing dominance of coal combustion in the Polish economy, which also applies to the urban municipal sector. As a consequence, particulate matter emissions of this sector are clearly predominant over the road traffic contribution. Balance can be achieved once the ongoing de-carbonization of the national economy has been completed.

Methane fermentation of organic waste is one way to minimize organic waste, which accounts for 77% of the global municipal waste stream. The use of biowaste treatment technologies helps to improve the energy independence of the regions. Improving the efficiency of the methane fermentation process by using additives from waste may be an attractive alternative to the original technology. The use of biochar as an additive for methane fermentation has been shown to increase the production potential of biogas. The reasons for the improvement in efficiency are complex among others, it is assumed that the specific surface area of ​​biochar may increase the population of anaerobic organisms. Up to date, there are many researches on the effect of biochar additions on methane fermentation, but there is no research on the effect of sulfur-biochar composite. The composite product in the form of a mixture of biochar and molten sulfur is an interesting area of ​​research. In this experiment additions of the sulfur-biochar composite were tested to improve the fermentation process. The composite consisted of 40% biochar and 60% of sulfur and was added to the process. As results the addition of 1% of the composite increased the biogas potential by 4%.

Peat is the main type of peloid used in Polish cosmetic/healing spa facilities. Depending on treatment and origin, peat waste can be contaminated microbiologically, and as result, it has to be incinerated in medical waste incineration plants without energy recovery (local law). Such a situation leads to peat waste management costs increase. Therefore in this work, we checked the possibility of peat waste upcycling to carbonized solid fuel (CSF) using torrefaction. Torrefaction is a thermal treatment process that removes microbiological contamination and improves the fuel properties of peat waste. In work torrefaction conditions (temperature and time) on CSF quality were tested. Parallelly, peat decomposition kinetics using TGA, and torrefaction kinetics with lifetime prediction using macro-TGA were determined. Furthermore, torrefaction theoretical mass and energy balance were determined. The results of the tested peat were compared with reference material (wood), and as result, obtained data can be used to adjust currently used wood torrefaction technologies for peat torrefaction. The results show that torrefaction can improve the high heating value of peat waste from 19.0 MJ x kg-1 to 21.3 MJ x kg-1, peat main decomposition takes place at 200-550 °C following second reaction order (n=2), with an activation energy of 33.34 kJ x mol-1 and pre-exponential factor of 4.40 x 10-1 s-1. Moreover, DSC analysis revealed that peat torrefaction required slightly more energy than wood torrefaction, and macro TGA showed that peat torrefaction has lower torrefaction constant reaction rates (k) than wood 1.05 x 10-5 - 3.15 x 10-5 vs 1.43 x 10-5 - 7.25 x 10-5 s-1.

Carrying out technological tests and research is indispensably connected with high costs and elongation of implementation time of new casting technologies and their modifications in industry. Hence, the application of specialised computer software, enabling the simulation and analysis of metallurgical and casting processes in modern foundry industry is of special interest. The present work presents an experimental application of MAGMASoft software in casting process of EV31A magnesium alloy structural element of helicopter engine. The results of computer simulation have been compared with industrial cast of the simulated element, fabricated in the same conditions. It has been revealed that the computer simulation provides a complex information on the analysed process, leading to reduction of costs and time, connected with technological tests in implementation of new casting technologies.

Background: Patients with infectious complications related to the presence of cardiac implantable electronic devices (CIED) constitute a heterogeneous group, including local pocket infection (PI) and lead related infectious endocarditis (LRIE). The diagnosis of CIED-related infection is often difficult and requires complex imaging and microbiological tests. The aim of the current study was to evaluate the usefulness of new simple hematological parameters in detecting infectious complications in patients with CIED and assessing their extent. Methods: The retrospective analysis of clinical data of 2909 patients (36.37% with CIED-related infections), undergoing transvenous lead extraction (TLE) procedures in three high-volume centres in the years 2006-2020, was conducted. The sensitivity and specificity of new hematological markers were evaluated: the neutrophil to lymphocyte ratio (NLR), neutrophil to platelet ratio (NPR), and lymphocyte to platelet ratio (LPR) in the diagnosis of infectious complications, assessment of the spread of the infectious process and differentiation of additional structures related to the presence of leads. Results: In patients with CIED infections, compared to the non-infectious group, high inflammatory parameters were confirmed, and the highest specificity of the new markers in detecting the infectious process was demonstrated. (79.47% for NPR and 72.82% for NLR). Analysis of laboratory parameters in infectious and non-infectious patients with the presence of additional lead-related masses showed the highest specificity of NPR (82,78%) in predicting the diagnosis of vegetation. Comparison of laboratory parameters in patients with LRIE and PI showed higher NLR % (4.24 vs 2.56; p<0.001, area under curve (AUC) in a receiver operating characteristic (ROC) curve: 0.67 and 0.78 respectively) and higher NPR (0.03 vs 0.02; p<0.001; AUC: 0,51 and 0,55) in patients with LRIE. The highest sensitivity in the detection of coexistence LRIE with PI has been demonstrated for NLR (87.33%). Conclusions: Novel hematological markers may be helpful in the recognition of the inflammatory process in patients with CIED, differentiation of vegetation and vegetation-like masses, and the extent of infection in patients with PI.

In this paper, particular attention is paid to an advanced variant of the plug-in hybrid engine, known as PHEV, which combines two functionalities: the vehicle's internal combustion engine (ICEV) and the electric engine (BEV). The paper also presents the assumptions of the DEVS model, which allows for the estimation of vehicle’s energy consumption. The study described herein also presents the influence of factors such as ambient temperature and constant speed on the PHEV’s energy consumption. It has been shown that the vehicle’s range estimated based on its electronic control module (ECU) is about 20% shorter per annum on average for its year-round operation in every-day driving conditions. When analyzing the energy consumption based on the vehicle’s unit energy consumption model, attention was paid to values that are strongly correlated with the traffic volume and weather conditions affecting the moving vehicle. In addition, the authors emphasized that the estimated total energy consumption of an electric (BEV) or hybrid (PHEV) vehicle, relative to the normative values arising from the type approval test cycle, deviate from the actual values arising from real driving conditions and often vary substantially. In extreme cases, the actual range of a hybrid vehicle is 75% shorter.

In the past few decades, the use of agricultural waste has been increasing due to the increasing demand for sustainable materials. This paper aims to review the significance of agricultural waste as a type of wealth and its potential as an alternative material in the composite field. It was found that many agricultural wastes had been utilized in various production of ecological products. The review discusses the physical, mechanical, and chemical properties of the natural fibers made from agricultural waste and the use of Pineapple Leaf Fiber Reinforced Composite to manufacture UAVs (Unmanned Aerial Vehicles). The extracted waste fibers’ physical, mechanical, and chemical properties show that these agricultural wastes can produce better advanced composite materials suitable for structural applications. The use of pineapple leaf fiber in Unmanned Aerial Vehicles manufacturing has the potential to revolutionize this industry and contribute to a more sustainable future.

The practice of the veterinary profession is regulated by public law and corporate regulations. The most fundamental corporate regulations of veterinary associations, to which membership is a condition of practising the profession, are the codes of veterinary ethics. The codes of ethics contain five basic rules for practising veterinary responsibilities to animals, clients, the public, the veterinary team, and the veterinarians themselves. Veterinarians are often faced with dilemmas about whose interests to protect; this is due to the tasks the profession carries out while protecting the animals of their owners and the public. Ethical principles are a guideline for veterinarians in difficult situations. Depending on the hierarchy of values imposed by codes of ethics, different decisions can be made. In this article, we present the importance that the Polish code of veterinary ethics gives to animal welfare as an obligation of veterinarians towards animals and compare how animal welfare is positioned in the European, American, British and New Zealand codes of veterinary ethics. In contrast to other codes, the Polish code of veterinary ethics does not sufficiently recognise animal welfare as a value to be protected in the practice of the profession.

Participatory Action Research (PAR) is an established method to implement change in organizations. However, it cannot be applied in the open source (FOSS) communities, without adaptation to their particularities, especially to the specific control mechanisms developed in FOSS. FOSS communities are self-managed, and rely on consensus to reach decisions. This study proposes a PAR framework specifically tailored to FOSS communities. We successfully applied the framework to implement a set of quality assurance interventions in the Robot Operating System community. The framework we proposed is composed of three components, interventions design, democratization, and execution. We believe that this process will work for other FOSS communities too. We have learned that changing a particular aspect of a FOSS community is arduous. To achieve success the change must rally the community around it for support and attract motivated volunteers to implement the interventions.

Mushroom production in Poland is an important and dynamically developing element of diverse agriculture. Mushroom spent compost (MSC) is major waste generated during production, i.e., MSC: mushrooms is ~5:1. To date, the main use of MSC is soil application as organic fertilizer. To date, several methods of MSC treatment have been researched and developed including production of compost, bioethanol, biogas, enzyme lactase, xylo-saccharides, and hydrogen. Torrefaction may be considered a novel approach for biomass valorization. Thus, we are pioneering the potential use of MSC valorization via torrefaction. We explored valorizing the waste biomass of MSC via thermal treatment – torrefaction (‘roasting’) to produce biochar with improved fuel properties. Here for the first time, we examined and summarized the MSC torrefaction thermogravimetric analyses, fuel properties data of raw biomass of MSC and biochars generated from MSC via torrefaction. The effects of torrefaction temperature (200~300 °C), process time (20~60 min), on fuel properties of the resulting biochars were summarized. The dataset contains results of thermogravimetric analysis (TGA) as well as proximate analyses of MSC and generated biochars. The presented data are useful in determining MSC torrefaction reaction kinetics, activation energy and to further techno-economical modeling of the feasibility of MSC valorization via torrefaction. MSC torrefaction could be exploited as part of valorization resulting from a synergy between an intensive mushroom production with the efficient production of high-quality renewable fuel.

Complex proteomic and physiological approaches to study cold and heat stress responses in plant mitochondria are still limited. Variations in the mitochondrial proteome of cauliflower (Brassica oleracea var. botrytis) curds after cold and heat and after stress recovery were assayed by 2D PAGE in relation to respiratory parameters. Quantitative analysis of the mitochondrial proteome revealed numerous stress-affected protein spots. In cold alternative oxidase isoforms were extensively upregulated; major downregulations in the level of photorespiratory enzymes, porine isoforms, oxidative phosphorylation (OXPHOS) and some low-abundant proteins were observed. On the contrary, distinct proteins, including carbohydrate metabolism enzymes, heat-shock proteins, translation, protein import, and OXPHOS components were involved in heat response and recovery. Few metabolic regulations were suggested. Cauliflower plants appeared less susceptible to heat; closed stomata in heat stress resulted in moderate photosynthetic, but only minor respiratory impairments, however photosystem II performance was unaffected. Decreased photorespiration corresponded with proteomic alterations in cold. Our results show that cold and heat stress not only operate in diverse mode (exemplified by cold-specific accumulation of some heat shock proteins), but exert some associations on molecular and physiological levels. This implies more complex model of action of investigated stresses on plant mitochondria.

In this study, 12 organic waste materials were subjected to TG/DTG thermogravimetric analysis and DSC calorimetric analysis. These analyses provided basic information about thermochemical transformations and degradation rates during organic waste pyrolysis. Waste materials were chosen to cover the most abundant waste that can be found in the municipal solid waste stream. Based on TG results, kinetic parameters according to Coats–Redfern method were determined. The pyrolysis activation energy was the highest for cotton 134.5 kJ∙(mol∙K)-1 and the lowest for leather 25.2 kJ∙(mol∙K)-1. The DSC analysis showed number of transformations occurred during pyrolysis for each material. For each transformation, normalized energy required for transformation, or released during transformation were determined and then summarized to present energy balance. The study found that only for three waste materials: PET (-220.1 J⸱g-1), leather (-66.9 J⸱g-1), and chicken meat (-130.3 J⸱g-1) energy balance was negative, while the highest positive balance value was found for potato peelings (367.8 J⸱g-1). The obtained results may be applied for the modelling of energy and mass balance of municipal solid waste pyrolysis.

Introduction: Due to the spread of SARS CoV-2 virus responsible for COVID-19 disease, there is an urgent need to analyse COVID-19 epidemic perception in Poland. This study aims to investigate social perception of coronavirus in the Internet media during the epidemic. It is a signal report highlighting the main issues in public perception and medical commutation in real time. Methods: We study the perception of COVID-19 epidemic in Polish society using quantitative analysis of its digital footprints on the Internet on platforms: Google, Twitter, YouTube, Wikipedia and electronic media represented by Event Registry, from January 2020 to 29.04.2020 (before and after official introduction to Poland on 04.03.20). We present trend analysis with a support of natural language processing techniques. Results: We identified seven temporal major clusters of interest on the topic COVID-19: 1) Chinese, 2) Italian, 3) Waiting, 4) Mitigations, 5) Social distancing and Lockdown, 6) Anti-crisis shield, 7) Restrictions releasing. There was an exponential increase of interest when the Polish government “declared war against disease” around 11/12.03.20 with a massive mitigation program. Later on, there was a decay in interest with additional phases related to social distancing and an anti-crisis legislation act with local peaks. We have found that declarations of mitigation strategies by the Polish prime minister or the minister of health gathered the highest attention of Internet users. So enacted or in force events do not affect interest to such extent. Traditional news agencies were ahead of social media (mainly Twitter) in dissemination of information. We have observed very weak or even negative correlations between a colloquial searching term 'antiviral mask' in Google, encyclopaedic definition in Wikipedia “SARS-CoV-2” as well official incidence series, implying different mechanisms governing the search for knowledge, panic related behaviour and actual risk of acquiring infection. Conclusions: Traditional and social media do not only reflect reality, but also create it. Risk perception in Poland is unrelated to actual physical risk of acquiring COVID-19. As traditional media are ahead of social media in time, we advise to choose traditional news media for a quick dissemination of information, however for a greater impact, social media should be used. Otherwise public information campaigns might have less impact on society than expected.

This paper presents a methodology for determining the sensitivity of the main air flow directions in ventilation subnetworks to changes of aerodynamic resistance and of air density in mine workings. Formulae for determination of the sensitivity of the main subnetwork air flows by establishing the degree of dependency of the air volume stream in a given working on the variations in resistance or air density of other workings of the network have been developed. They have been implemented in the VentGraph mine ventilation network simulator. This software, widely used in Polish collieries provides an extended possibility to predict the process of ventilation, air distribution and, in the case of underground fire, also the spread of combustion gasses. The new method facilitates assessment by mine ventilation services of the stability of ventilation systems in exploitation areas and determine of the sensitivity of the main subnetwork air flow directions to changes of aerodynamic resistance and air density. Recently in some Polish collieries new longwalls are developed in seams located deeper then the bottom of the intake shaft. Such solution is called “exploitation below the level of access” or “sublevel”. The new approach may be applied to such developments to assess the potential of changes of direction and air flow rates. In addition, interpretation of the developed sensitivity indicator is presented. While analyzing air distributions for sublevel exploitation, application of current numerical models for calculations of the distribution results in tangible benefits, such as the evaluation of the safety or risk levels for such exploitation. Application of the VentGraph computer program, and particularly the module POŻAR (fire) with the newly developed options, enables an additional approach to the sensitivity indicator in evaluating air flow safety levels for the risks present during exploitation below the level of the intake shaft. The analyses performed and examples presented enabled useful conclusions in mining practice to be drawn.

Traumatic brain injury (TBI) is an important global clinical issue, requiring not only prevention but also effective treatment. Following TBI, diverse parallel and intertwined pathological mechanisms affecting biochemical, neurochemical, and inflammatory pathways can have severe impact on the patient’s quality of life. The current review summarizes evidence for utility of amantadine in TBI in connection to its mechanism of action. Amantadine combining multiple mechanisms of action may offer both neuroprotective and neuroactivating effects in TBI patients. Indeed, use of amantadine in TBI has been encouraged by several clinical practice guidelines/recommendations. Amantadine is also available as infusion which may be of particular benefit in unconscious patients with TBI, due to immediate delivery to the central nervous system and the possibility of precise dosing. In other situations, orally administered amantadine may be used. There are several questions that remain to be addressed: Can amantadine be effective in disorders of consciousness requiring long-term treatment and in combination with drugs approved for treatment of TBI? Do the observed beneficial effects of amantadine extend to disorders of consciousness due to factors other than TBI? Well controlled clinical studies are warranted to ultimately confirm its utility in the TBI and provide answers these questions.

Humans are a vision-dominated species, and what we see depends on where we look. Therefore, eye movements (EM) are essential to our interactions with the environment, and experimental findings show EM is affected in neurodegenerative disorders (ND). It could be a reason for some cognitive and movement disorders in ND. Therefore, we aim to determine if changes in EM-evoked responses can tell us about ND, such as Alzheimer’s (AD) and Parkinson’s Disease (PD) progression in different stages. In the present review, we have analyzed the results of neurological, psychological, and EM (saccades, antisaccades, pursuit) tests to predict disease progression with Machine Learning (ML) methods. Described predictive algorithms are using various approaches, including Granular Computing, Naive Bayes, Decision Trees/Tables, Logistic Regression, C-/LinearSVC, KNC, and Random Forest. We demonstrated that EM is a robust biomarker for assessing symptom progression in PD and AD. There are also navigation problems in 3D space in both diseases. Consequently, we investigated EM experiments in the virtual space and how they may help find neurodegeneration-related brain changes. In conclusion: EM parameters with clinical symptoms are powerful precision instruments that, in addition to predictions of ND progression with the help of ML, could be used to indicate the different preclinical stages of both diseases.

Agrilus planipennis is a devastating invasive pest of ash trees. This wood-boring insect native to Asia and established in European Russia about 20 years ago poses a serious threat to ash trees all over Europe. In 2019 we first detected Agrilus planipennis in Ukraine. More than 20 larvae have been collected from under the bark of Fraxinus pennsylvanica trees on 5 September 2019 in Markivka District of Luhansk Region. Coordinates of the localities of collection: 49.614991 N, 39.559743 E; 49.614160 N, 39.572402 E and 49.597043 N, 39.561811 E. The photos of damaged trees with larval galleries, exit holes and larvae are presented. There is no doubt that the pest is established in Ukraine. This fact is important for development of quarantine protocols to prevent or at least slow the further spread of this invasive pest in Europe.

Despite the development of biorefinery processes, so far this area does not take into account the possibility of coupling the “conventional” composting process with the production of biochemicals. However, net carbon monoxide (CO) production has been observed during bio-waste composting. So far, O2 concentration and temperature have been identified as main variables influencing CO formation. This study aimed to investigate the CO net production during bio-waste composting under controlled laboratory conditions, depending on different aeration rates and temperatures. A series of composting processes were carried out in conditions ranging from ~psychrophilic to thermophilic (T=35, 45, 55, and 65°C) and an aeration rate of 2.7, 3.4, 4.8, and 7.8 L‧h-1. The highest concentrations of CO in each thermal variant was achieved with oxygen deficit (aeration rate 2.7 L‧h-1); additionally, CO levels increased with temperature, reaching ~300 ppm at 65°C. Production of CO in mesophilic and thermophilic conditions draws attention to the biological CO formation by microorganisms capable of producing the CODH enzyme. Further research on the CO production efficiency in these thermal ranges is necessary with the characterization of the microbial community and analysis of the ability of the identified bacteria to produce the CODH enzyme and convert CO from CO2.

To fulfil a need of limitation automotive emissions the reduction of vehicle weight is widely recommended and achieved in many ways, both by the construction of individual elements of the vehicle, and by the selection of light materials including Al alloys. Connecting these elements with each other and with elements made of iron alloys can be realized, inter alia, by welding or stir welding. However, the quality of the welds obtained varies widely and depends on many design, operational and environmental factors. The present study focused on a review of various welding technics used to joint both similar and dissimilar Al alloys utilized in automotive industry, the effect of various process parameters on welds quality, and phenomena appeared in such welds..

The paper presents a new procedure for assessing the polarity and hydrophobicity of deep eutectic solvents (DESs) based on the measurement of the DES contact angle on glass. DESs consisting of benzoic acid derivatives and quaternary ammonium chlorides – tetrabutylammonium chloride (TBAC) and benzyldimethylhexadecylammonium chloride (16-BAC) in selected mole ratios were chosen for the study. To investigate the DESs polarity, an optical goniometer and an ET(30) solvatochromic scale based on the Reichardt’s dye were used. The research demonstrated the high accuracy and precision of the developed procedure. The simplicity of the examination and the availability of basic equipment allow the implementation of the developed procedure in routine investigations of DESs.

Biowaste generated in the process of Oxytree cultivation and logging represents a potential source of energy. Torrefaction (a.k.a. low-temperature pyrolysis) is one of the methods proposed for the valorization of woody biomass. Still, energy is required for the torrefaction process during which the raw biomass becomes biochar with fuel properties similar to lignite coal. In this work, models describing the influence of torrefaction temperature and residence time on the resulting fuel properties (mass and energy yields, energy densification ratio, organic matter and ash content, combustible parts, lower and higher heating values, CHONS content, H:C and O:C ratios) were proposed according to the Akaike criterion. The degree of the models’ parameters matching the raw data expressed as the determination coefficient (R²) ranged from 0.52 to 0.92. Each model parameter was statistically significant (p<0.05). Estimations of the value and quantity of the produced biochar from 1 Mg of biomass residues were made based on two models and a set of simple assumptions. The value of torrefied biochar (€123.4·Mg^-1) was estimated based on the price of commercially available coal fuel and its lower heating value (LHV) for biomass moisture content of 50%, torrefaction for 20 min at 200 °C. This research could be useful to inform techno-economic analyses and decision-making process pertaining to the valorization of pruned biomass residues.

Mitochondrial dysfunction has been linked to psoriasis, and may be an important underlying factor contributing to this disease. However, a precise methodology for assessing mitochondrial dysfunction has yet to be developed. One promising approach is to measure NADH autofluorescence from affected skin areas. In this study, we show that Flow Mediated Skin Fluorescence (FMSF) can be used for non-invasive assessment of mitochondrial dysfunction in psoriasis. The fluorescence level at baseline and the half-time of ischemic growth (t1/2) derived from the FMSF traces can be used for non-invasive assessment of NADH/NAD+ redox imbalance in psoriatic lesions compared to unaffected skin. These results are supported by analysis of the key FMSF parameters, Reactive Hyperemia Response (RHR) and Hypoxia Sensitivity (HS). This method not only contributes to understanding of the biochemical processes involved in the etiopathogenesis of psoriasis, but also provides a basis for identifying new drug targets and improving the treatment process.

In this paper, the characterization of 3D printed materials that are considered in the design of multirotor unmanned aerial vehicles (UAVs) for specialised purposes was carried out. The multirotor UAV system is briefly described, primarily from the aspect of system dynamics, considering that the airframe parts connect the UAV components, including the propulsion configuration, into a functional assembly. Three additive manufacturing (AM) technologies were discussed, and a brief overview was given of selective laser sintering (SLS), fused deposition modeling (FDM), and continuous fiber fabrication (CFF). Using hardware and related software, 12 series of specimens were produced which were experimentally tested utilizing a quasi-static uniaxial tensile test. The results of the experimental tests are given graphically with displacement-force characteristics. In this work, the focus is on CFF technology and the testing of materials that will be used in the production of mechanically loaded airframe parts of multirotor UAVs. Furthermore, an overview was given in such a way that the specimens were grouped, and the mean values of the maximum stress were presented, so that the tested materials could be more easily compared with conventional materials, such as aluminum alloys.

Is HSP-27 an emerging marker of good prognosis in septic shock patients – a pilot study Objective To estimate the value of serum changes of C-reactive protein, procalcitonin, presepsin, heat shock protein 27 (HSP27) and neutrophil to lymphocyte ratio in assessing the prognosis in patients with septic shock (SS) treated in intensive care unit. Methods The SS was diagnosed and treated in accordance with the guidelines of the Surviving Sepsis Campaign. 37 selected adult patients with SS were included. Serum concentrations of biomarkers were measured at admission and daily for 4 consecutive days (time points T0,T1, T2, T3 and T4 respectively). The mortality rate was determined 28 days after admission. Patients were divided into survivor and non-survivor groups according to their mortality. The differences between the levels of biomarkers at the T0 and T4 time points were analyzed. Results The mean value of the SOFA score on admission was 11.7 ± 2.7, and the APACHE II scale 29.9 ± 6.85. Nine patients died. Univariate logistic analysis revealed that changes between T0 and T4 time points of presepsin, procalcytonin, and HSP27 were associated with prognosis. A multivariate Cox analysis showed that an increase in HSP27 on T4 was the only independent predictor of good prognosis in SS patients. The area under the receiver operating characteristics curve for HSP27 was 0.785. Kaplan–Meier analysis showed that the mortality was lower (p=0.014) in patients who had an increase in HSP27 on T4 compared to those whose serum HSP27 did not increase on T4. Conclusions The increase of HSP27 level on the 4th day predicts favorable outcome in SS patients.

The proof-of-the-concept of application of low-temperature food waste biochars for the anaerobic digestion (AD) of food waste (the same substrate) was tested. The concept assumes that residual heat from biogas utilization may be reused for biochar production. Four low-temperature biochars produced under two pyrolytic temperatures 300 °C and 400 °C and under atmospheric and 15 bars pressure with 60 minutes retention time were used. Additionally, the biochar produced during hydrothermal carbonization (HTC) was tested. The work studied the effect of a low biochar dose (0.05 gBC x gTSsubstrate-1, or 0.65 gBC x L-1) on AD batch reactors’ performance. The biochemical methane potential test took 21 days and the process kinetics using the first-order model were determined. The results showed that biochars obtained under 400°C with atmospheric pressure and under HTC conditions improve methane yield by 3.6%. It has been revealed that thermochemical pressure influences the electrical conductivity of biochars. The biomethane was produced with a rate (k) of 0.24 d-1, and the most effective biochars increased the biodegradability of FW to 81% in comparison to variants without biochars (75%).

The data from the extended system was recorded on-going basis in the archives of the monitoring system, and the multipoint system was directly stored in the methane-anemometers, which allows one to perform a comparative analysis of the obtained records from many methane detectors. An important element of the analyses was the simultaneous registration of the methane concentration in the longwall, together with information on operating time, direction of shearer operation and type of work: mining/cleaning. Mining practice shows that in methane coal mines, methane is often the basic limitation for modern and high-efficiency longwall complexes. The paper reports on selected observations and tests carried out in the Cw-4 longwall in seam 364/2 of the Budryk mine belonging to JSW SA. In observation of the methane level in the Cw-4 longwall, additional sensors installed in the end part of the longwall from the side of the gobs and registration of air velocity and methane concentration in the multipoint system built in the selected longwall cross-section were used, regardless of the air parameters recorded in the monitoring system.

Mink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). RT-PCR was used to detect astrovirus genetic material, and the reaction products were separated on a 1% agarose gel. The specificity of the reaction was confirmed by sequencing all samples. The presence of astrovirus RNA was detected in each of the samples tested. Sequencing and bioinformatic analysis indicated the presence of the same variant of the virus in all samples. Comparison of the variant with the sequences available in bioinformatics databases confirmed that the Polish isolates form a separate clade, closely related to Danish isolates. The similarity of the Polish variant to those isolated in other countries ranged from 2.4% (in relation to Danish isolates) to 7.1% (in relation to Canadian isolates). Phylogenetic relationships between variants appear to be associated with the geographic distances between them. To our knowledge, this work describes the first results on the molecular epidemiology of MAstV in continental Europe. The detection of MAstV in Central Europe indicates the need for further research to broaden our understanding of the molecular epidemiology of MAstV in Europe.

Physical activity (PA) is a factor that may have an influence on the symptoms of Parkinson’s disease (PD). The aim of this study was to identify the potential determinants of spontaneous PA in the PD patient group. 134 PD patients aged 65.2±9.2 years, Hoehn-Yahr scale ≤ 4, Mini Mental State Examinaton (MMSE) ≥ 24 were examined. For the study purposes, the authors analyzed: age, sex, education, history of PD, dopaminergic treatment, the severity of PD symptoms using Unified Parkinson’s Disease Rating Scale (UPDRS) and Hoehn-Yahr scale. Additionally all participants were evaluated through a set of scales for specific neuropsychiatric symptoms including: depression, anxiety, apathy, fatigue and sleep disorders. An analysis of linear regression was used with backward elimination. In the total explanatory model, 12% of the variability in activity (R2=0.125; F(16.133)=2.185; p<0.01), the significant predictor was starting therapy with the dopamine agonist (DA) (β= 0.420; t= 4.068; p=0.000), which was associated with a longer duration of moderate PA. In the total explanatory model, for more than 13% of the variance in time spent sitting (R2=0.135; F(16.130)=2.267; p<0.01), the significant predictors were secondary education and the results of the UPDRS. The patients with secondary and vocational education, those starting treatment with DA and those with a less severe degree of Parkinson’s symptoms (UPDRS) spent less time sitting in a day. It is possible to identify determinants of spontaneous PA. It may elucidate consequences in terms of influence on modifiable conditions of PA and the proper approach to patients with unmodifiable PA factors.

The nondestructive testing of reinforced concrete chimneys, especially the high ones, is an important element of the assessment of their condition, making it possible to forecast their safe service lifespan. Industrial chimneys are often exposed to the strong action of acidic substances – they are adversely affected by the flue gas condensate on the inside and by acid precipitation on the outside. Initially, this results in the corrosion of the shell concrete and then in the corrosion of the reinforcing steel. During the service life of such chimneys their condition should monitored in order to prevent structural failures and indicate the most endangered parts of the structure. Owing to thermographic surveys one can monitor the hazards leading to the degradation of the chimney structure, which is particularly vital when due to the character of the production process the chimney cannot be put out of operation. The methods for the interpretation of results from thermovision studies to determine the safety and durability of industrial chimneys are shown.

In this paper, the ductile fracture mechanism is discussed. The results of the numerical and experimental analyses are used to estimate of the onset of the crack front growth . It is assumed that the ductile fracture in front of the crack starts at the location along the crack front where the accumulated effective plastic strain reaches a critical value. It is also assumed that the critical effective plastic strain depends on the stress triaxiality and the Lode angle. The experimental programme was performed using five different specimen geometries, three different materials and three different temperatures of +20°C, -20°C and -50°C. Using the experimental data and the results of the finite element computations, the critical effective plastic strains are determined for each material and each temperature. However, before the critical effective plastic strain is determined, a careful calibration of the stress–strain curves was performed after modification of the Bai–Wierzbicki procedure. Finally, by analysing the experimental results recorded during the interrupted fracture tests and scanning microscopy observations, the research hypothesis is verified.

Background: Dual function tail tubular proteins (TTP) belonging to the lytic bacteriophages are the interesting group of biologically active enzymes. Surprisingly, apart from their structural function, they are also polysaccharide hydrolyzes destroying bacterial extracellular components. One of the representatives of this group is TTPB from Klebsiella pneumoniae phage – KP32. TTPB hydrolyzes exopolysaccharide (EPS) of Klebsiella pneumoniae and Enterococcus faecalis strain. This depolymerizing feature was associated with the activity to prevent bacterial biofilm formation. TTPB can inhibit biofilm formation by K. pneumoniae, Enterobacter cloacae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa strains. Moreover, synergistic activity with antibiotic action has been observed, most likely due to depolymerases’ facilitation of contact of antibiotic with bacterial cells. Methods: TTPB was overexpressed in E coli system, purified and tested towards the bacterial strains using agar overlay method. The hydrolytic activity of TTPB was performed using EPSs of K. pneumoniae PCM2713 and E. cloacae ATCC 13047 as the substrates. Next, we determined the reducing sugar (RS) levels in the TTPB/EPS mixtures, regarding the RS amount obtained after acidic hydrolysis. The antibiofilm activity of TTPB has been set down on bacterial biofilm using a biochemical method. Finally, we have demonstrated the synergistic activity of TTPB with kanamycin. Results: For the first time, the hydrolytic activity of TTPB towards bacterial EPSs has been shown. TTPB releases about a half of the whole RS amount of EPSs belonging to K. pneumoniae PCM 2713 and E. cloacae ATCC 13047 strains. 1.12 µM of the phage protein reduces biofilm of both strains by over 60%. Destroying the bacterial biofilm the phage protein improves the antibiotic action increasing kanamycin effectiveness up to four times.

The effect of configuration of an asymmetric bulky initiator 2,2’-[1,1’-binaphtyl-2,2’-diyl- bis-(nitrylomethilidyne)]diphenoxy aluminum isopropoxide (Ini) on structure of copolymer of asymmetric monomer L,L-lactide (Lac) with symmetric comonomer trimethylene carbonate (Tmc) was studied using polarimetry, dilatometry, SEC and ¹³C NMR. When the S-enantiomer of Ini was used the distribution in copolymer chains at the beginning of polymerization is statistical, with alternacy tendency, changing next through a gradient region to homoblocks of Tmc. When, however, R-Ini was used, the product formed was a gradient oligoblock one, with Tmc blocks prevailing at the beginning, changing to Lac blocks dominating at end part of chains. Initiation of copolymerization with the mixture of both initiator enantiomers (S:R = 6:94) gave multiblock copolymer, of similar features but shorter blocks. Analysis of copolymerization progress required complex analysis of dilatometric data, assuming different molar volume contraction coefficients for units located in different triads. Comonomer reactivity ratios of studied copolymerizations were determined.

The description of protein disordered states is important for understanding protein folding mechanisms and their functions. In this short review, we briefly describe a simulation approach to modeling disordered protein interactions and unfolded states of globular proteins. It is based on the CABS coarse-grained protein model that uses a Monte Carlo (MC) sampling scheme and a knowledge-based statistical force field. We review several case studies showing that description of protein disordered states resulting from CABS simulations is consistent with experimental data. The case studies comprise investigations of protein-peptide binding and protein folding processes. The CABS model has been recently made available as the simulation engine of multiscale modeling tools enabling studies of protein-peptide docking and protein flexibility. Those tools offer customization of the modeling process, driving the conformational search using distance restraints, reconstruction of selected models to all-atom resolution and studies of large protein systems in a reasonable computational time. Therefore, CABS can be combined in integrative modeling pipelines incorporating experimental data and other modeling tools of various resolution.

If a lubricant contains structures capable of conducting energy, reactions involving zinc dialkyldithiophosphate (ZDDP) may take place both very close to and away from the solid surfaces, with this indicating that ZDDP can be a highly effective anti-wear (AW) additive. The central thesis of this article is that the tribocatalytic effect is observed only when the energy emitted by the solids is transmitted by ordered molecular structures present in the lubricant, e.g., graphene. The friction tests were carried out for 100Cr6 steel balls in a sliding contact with uncoated or a:C-H-coated HS6-5-2C steel discs in the presence of polyalphaolefin 8 (PAO 8) as the lubricant, which was enhanced with graphene and/or ZDDP. There is sufficient evidence of the interactions occurring between ZDDP and graphene and their effects on the tribological performance of the system. It was also found that the higher the concentration of zinc in the wear area, the lower the wear. This was probably due to the energy transfer resulting from the catalytic decomposition of ZDDP molecules. Graphene, playing the role of the catalyst, contributed to that energy transfer.

Genes encoding retinol binding protein 4 (RBP4), follicle stimulating hormone subunit beta (FSHB) and epidermal growth factor (EGF) have been proposed as candidate genes for reproduction traits in pigs. The study presented in this paper aimed to find associations between variants of these genes and reproduction traits in pigs reared in Poland. Investigation included Polish Large White x Polish Landrace (n=288) and Yorkshire (n=195) sows. Individual genotypes were determined by means of PCR-RFLP (single nucleotide polymorphism in RBP4, FSHB) or PCR (insertion/deletion in EGF) methods. Obtained result showed that RBP4 variants were related to total number born (TNB) and number born alive (NBA) in 1st, 2nd and 5th parities, FSHB to TNB and NBA in first two parities, however EGF with both traits in all parities (P≤0.05 or P≤0.01). In case of RBP4 gene, heterozygous genotype (AB) was favorable for analyzed traits, however in case of FSHB and EGF homozygous genotype, AA and BB respectively. Obtained results indicate that polymorphism in three analyzed genes is associated with important reproductive traits of pigs kept in Poland.

Introduction: Cleft lip with or without cleft palate (CL/P) is the most common congenital face anomaly in the world of multifactorial etiology. Objective: To determine the prevalence and characteristics of cleft palate. To identify associated risk factors to guide prevention and/or prenatal detection for early intervention and better health outcomes. To assess the rehabilitation protocols after operations in cleft palate. Design: Retrospective descriptive and clinical study. Patients: One hundred and three children with cleft palate treated in the Department of Head and Neck Surgery Clinic for Children and Young Adults, Department of Clinical Pediatrics, University of Warmia and Mazury, were included in the study. Methods: Records evaluation was conducted regarding sex, cleft type, mother occupation, parents education, histories of cleft in any of parents. The data were analyzed using R (R core team 2021) and the ordinal logistic regression analyses. Results: Children of stressed mothers during pregnancy had 9.4 times higher odds of having bilateral cleft. While there was no evidence that children sex, order of birth, and body mass as well as risk of maternal toxicity at work or reporting infections or drugs toxicity had a significant effect on the dependent variable. Conclusions: Children of parents with cleft history and having low educated mothers had higher probability of bilateral cleft palate. Children of stressed mothers during pregnancy had higher odds of having bilateral cleft. In literature, there is a lack of reports about rehabilitation in different operations methods in children with cleft palates.

One way to reduce the negative impact of human activity on the natural environment is to use natural, easily available and relatively cheap to produce compounds in industrial processes. One such compound is naturally occurring calcium carbonate (CaCO3). This compound has adsorption properties so that it can be an alternative to commonly used adsorbents. The aim of this work is to determine the possibility of using CaCO3 to remove pharmaceutical substances such as sulfadiazine and tetracycline from water. The CaCO3 used in this work was synthesised using our own method, which allows the production of CaCO3 particles with nanometric size. In the conducted research, calcium carbonate was used in the form of a suspension in purified solutions and as an inorganic filling of the developed membranes. The mass of pharmaceutical substances removed from their aqueous solutions was determined in the tests carried out. Based on the results obtained, it can be concluded that CaCO3 has the ability to adsorb both tetracycline and sulfadiazine. In suspension tests, the mass of the removed substances per unit mass of adsorbent was 1.52 mg/g and 6.85 mg/g, respectively. In turn, in the case of the process using the developed membranes, the mass of the removed substances per unit mass of adsorbent was 232 mg/m2 and 524 mg/g, which is a several times greater increase in the mass of the removed substance compared to the process using an unmodified membrane.

Economic development and population growth lead to increased production of chicken manure (CM), which is a problematic organic waste for its amount, environmental threats, and moisture content. There are different ways of CM, namely anaerobic digestion, composting, combustion, and direct land spreading. Hydrothermal carbonization (HTC) is another emerging way, however. In this study, the HTC of CM was performed to produce energy-rich material called hydrochar (HC). The effects of HTC temperature (180, 240, 300 C) and process time (30, 90, 180 min) were summarized. Proximate and ultimate analysis, as well as heating values (HHV, LHV), have been performed both on raw CM and derived HC. Additionally, the process performance has been examined. The obtained results show that HTC is a feasible method for CM disposal and valorization. Although process time did not influence considerably fuel properties of CM, higher temperature led to significantly higher HHV, reaching 23,880.6734.56 Jg-1 at 300 C and 180 min with an improvement of 8,329 Jg-1 compared with raw CM (15,551.67 Jg-1). The process conducted at 240 C in 30 min has been specified as the most favorable, due to the highest energy gain of HC and relatively low energy consumption.

Background: To investigate the distribution of refractive errors and their characteristics in older adults. Methods: The study design was cross-sectional study. A total of 1107 men and women were interviewed and underwent detailed ophthalmic examinations, 998 subjects underwent refraction. Myopia was defined as spherical equivalent (SER) refraction ≤ −0.5 dioptres (D) and hyperopia was defined as SER ≥ +0.5 dioptres (D). Results: Among those who were refracted the distribution of myopia and hyperopia was 24.1% (95% CI 21.4–26.7) and 37.5% (95% CI 34.5–40.5), respectively. Myopia decreased from 28.7% in subjects aged 35-59 years to 19.3% in those aged 60 years or older and hyperopia increased from 21.8% at 35–59 years of age to 53.3% in subjects aged ≥60 years. Multiple regression analysis showed decreasing age (OR 0.98, 95% CI 0.96–1.00), female gender (OR 1.87, 95% CI 1.18–2.95) and presence of cataract (OR 2.40 , 95% CI 1.24–4.63) were independent risk factors associated with myopia. Conclusions: The distribution of refractive errors found in our study is similar to those reported in other Caucasian populations and differs from Asian populations. Myopia was positively associated with younger age, female gender and presence of cataract.

Atopic dermatitis (AD) is common inflammatory dermatosis, typically with chronic and recurrent course, which significantly reduces the quality of life. Sleep disturbances are considered to be remarkably burdensome ailments in the patients with AD, and are routinely included during assessment of disease severity. Therefore, endogenous substances engaged in the control of circadian rhythms might be important in pathogenesis of AD and, possibly, be used as biomarkers of disease severity or even in development of novel therapies. Melatonin (MT), the indoleamine produced by pineal gland (but also by multiple other tissues, including skin), plays a pivotal role in maintaining the sleep/wake homeostasis. Additionally, it possess strong antioxidant and anti-inflammatory properties, which might directly link chronic skin inflammation and sleep abnormalities characteristic of AD. The objective of this work is to systematically present and summarize the results of studies (both experimental and clinical) that investigated the role of MT in the AD, with focus on the antioxidant and immunomodulatory effects of MT.

Background The falsification of food products is a practice that has always been with mankind. Initially, it stemmed from the need to satisfy basic needs, and then from the desire for profit, at the expense of the health and sometimes the lives of consumers. Today, food safety issues are becoming a priority in consumers' daily lives. It is estimated that one in four food products is falsified. Man has lost the ability to naturally identify healthy food. He has therefore begun to prioritise the speed and ease of its preparation at the expense, often of health. The techniques currently available for detecting falsification are inaccessible to the ordinary consumer. The technique proposed by the authors is the beginning of a publicly available method for detecting falsification. Scope and approach Currently, instrumental or sensory methods are used to assess the authenticity, and therefore identification, of foods. The former are expensive and time-consuming and the latter are unobjective. The authors proposed a method using ultrasound with parameters that are neutral to the material to be tested. The technique allows results to be obtained in times measured in microseconds without destroying the material under test, saving costs. Three differently weighted groups of beers were used for the study. These were industrial, regional and domestic beers. Main findings and conclusions The results of the study indicate that the ultrasonic method is useful for the non-destructive identification of industrial and regional beers, which are produced using different technologies and differ in composition. The method analysed was not suitable for separating homebrew beers from other types of beer.

Herein, a novel cavity design of racetrack integrated circular cavity established on metal-insulator-metal (MIM) waveguide is suggested for refractive index sensing application. Over the past few years, we have witnessed several unique cavity designs to improve the sensing performance of the plasmonic sensors created on the MIM waveguide. The optimized cavity design can provide the best sensing performance. In this work, we have numerically analyzed the device design by utilizing the finite element method (FEM). The small variations in the geometric parameter of the device can bring a significant shift in the sensitivity and FOM of the device. The best sensitivity and FOM of the anticipated device are 1400 nm/RIU and ~12.01, respectively. We believe that the sensor design analyzed in this work can be utilized in the on-chip detection of biochemical analytes.

The C/N ratio is an extremely important parameter in the composting process, which is directly responsible for the growth of microorganisms. A low C/N ratio contributes to higher emissions of greenhouse gases and odorous substances, such as ammonia (NH3), which is formed by nitrogen mineralization. Due to the highly toxic effects of ammonia, it is a particularly unwanted by-product that can disrupt the composting process because it poisons microorganisms and cause environmental issues. The activity of anammox bacteria, so far analyzed only in wastewater treatment processes, is a particularly efficient method of nitrogen removal, having an advantage over the conventional methods used previously. Our study proofs the presence of anammox bacteria during composting, what gives an opportunity to improve the process and reduce its impact on atmospheric pollution. Despite the aerobic nature of this process, the composted mass of waste presents conditions conducive to the development of these ammonia oxidizing bacteria, as well as other strains of microorganisms cooperating with them. This makes it possible to compost at a low C/N ratio; in addition, there is no need for additional energy supply through aeration, as the processes carried out by anammox bacteria do not require oxygen.

Registry and survey data gave an opportunity to compare possible roles of social attitudes and social values in control and limit the COVID-19 pandemic. We analysed social values and time series of daily R0 (reproduction rate) estimates for 27 European countries. Embedded and affective autonomy are significantly negatively associated (p-Value<0.05) with controlled R0. It could mean that well interconnected societies with high attention to maximize utilities are more likely to couple with COVID-19. Hierarchy is significantly positively associated (p-Value<0.05) with controlled R0. Thus, hierarchical structures could inhibits control of COVID-19. The results emphasize the need to pay more attention to social values context in evaluating the effectiveness of mitigation strategies internationally.

Obesity is one of the factors leading to the development of atherosclerosis. This metabolic disorder is associated with an increased production of reactive oxygen species, which affect the oxidative stress level. The aim of this study was to evaluate oxidative/antioxidative status and to investigate the correlation between redox markers and anthropometric parameters and body composition in adult patients after myocardial infarction and in individuals without a cardiovascular event in the past. Descriptive data on socio-demographic, clinical, and anthropometric features and blood samples were collected and categorized into two equal groups: after myocardial infarction (study group (SG), n = 80) and without a cardiovascular event (control group (CG), n = 80). The oxidative/antioxidative status was assessed in plasma on the basis of total oxidative/capacitive status (PerOx), total antioxidative status/capacity (ImAnOx), and oxidized low-density lipoprotein (oxLDL). OxLDL was significantly higher in the CG group compared to the SG group (p = 0.02). No significant differences were found with regard to PerOx and ImAnOx values between the studied groups. Significant positive correlation between PerOx and percentage of adipose tissue (FM [%]) and body adiposity index (BAI) was found in the two studied groups. ImAnOx significantly positively correlated with VAI in SG and FM% in CG. OxLDL negatively correlated with body mass index and waist to hip circumference ratio in CG. The total oxidative/antioxidative status is related to the amount of adipose tissue and the BAI of the subjects. It was observed that it correlates more frequently with the visceral distribution of body fat.

On-stream inspections are the most appropriate method for routine inspections during plant operation without going through production downtime. Ultrasonic inspection, one of the on-stream inspection methods, faces challenges when performed at high temperatures exceeding the recommended 52°C. This study aims to determine the ultrasonic velocity and attenuation with the known material grade, thickness, and temperatures by comparing theory calculation versus experiment with temperatures ranging between 30°C to 250°C on low carbon steel covering most petrochemical equipment material and working conditions. The theoretical analysis was to obtain Young's modulus, Poisson's ratio, and longitudinal velocity at different temperatures. The experiments validated the predicted results of ultrasonic change due to temperature increase and showed a maximum error of 3%. The experimental data of velocity and decibel change from the temperature range provide a reference for the future when dealing with unknown materials information on site that require a quick corrosion status determination.

Transseptal puncture is an established technique for achieving left atrium access during cardiac procedures. It is associated with low overall complication rate and high success rates. Although being almost a century old technique, recent years brought a substantial development in the field, allowing for faster and safer left atrium catheterization. In this paper we discuss its history, complications, available techniques and current state of the art.

(1) Background: The goal of our study was to identify SNPs, metabolic pathways (KEGG), and gene ontology (GO) terms significantly associated with calving and workability; (2) Methods: Based on the EuroGenomics reference data set, we analyzed direct (DCE) and maternal (MCE) calving ease, direct (DSB), and maternal (MSB) stillbirth, milking speed (MSP), and temperament (TEM). We estimated SNP effects using a multi-SNP mixed-model. Further, SNP positions were mapped to genes, and GO terms/KEGG pathways of the corresponding genes were assigned. The estimation of GO term/KEGG pathway effects was based on a mixed-model using SNP effects as dependent variables; (3) Results: The number of significant SNPs comprised 59 for DCE, 25 for DSB and MSP, 17 for MCE and MSB, and 7 for TEM. Significant KEGG pathways were found for MSB (2), TEM (2), and MSP (1), while 11 GO terms were significant for MSP, 10 for DCE, 8 for DSB and TEM, 5 for MCE, and 3 for MSB.; (4) Conclusions: From the perspective of a better understanding of the genomic background of the phenotypes, traits with low heritabilities suggest that the focus should be moved from single genes to metabolic pathways or gene ontologies significant for the phenotype.

The early generative phase of cauliflower (Brassica oleracea var. botrytis) curd ripening is sensitive to the water deficit. Mitochondrial responses under drought within Brassica genus are poorly understood. The main goal of this study was to investigate the mitochondrial biogenesis of three cauliflower cultivars varying with drought tolerance. Diverse quantitative changes (down-regulations mostly) in the mitochondrial proteome were assayed by 2D PAGE coupled with LC-MS/MS. Respiratory (e.g. CII, CIV and ATP synthase subunits), transporter (including diverse porin isoforms) and matrix multifunctional proteins (e.g. components of RNA editing machinery) appeared diversely affected in their abundance under two drought levels. Western immunoassays showed also cultivar-specific responses of selected mitochondrial proteins. Dehydrin-related tryptic peptides found in few 2D spots that appeared immunopositive with dehydrin-specific antisera highlighted the relevance of mitochondrial dehydrin-like proteins for the drought response. The level of selected messengers participating in drought response was also determined. We conclude that the mitochondrial biogenesis was strongly, but diversely affected in various cauliflower cultivars and associated with drought tolerance on the proteomic and functional levels. However, transcriptomic and proteomic regulations were largely uncoordinated due to the suggested altered availability of messengers for translation, mRNA/ribosome interactions and/or miRNA impact on transcript abundance and translation.

Unmanned aerial vehicle (UAV) usage is increasing drastically worldwide as UAVs are used in various industries for many applications, such as inspection, logistics, agriculture, and many more. This is because performing a task using UAV makes the job more efficient and reduces the workload needed. However, for a UAV to be operated manually or autonomously, the UAV must be equipped with proper safety features. An anti-collision system is one of the most crucial and fundamental safety features that UAVs must be equipped with. The anti-collision system allows the UAV to maintain a safe distance from any obstacles. The anti-collision technologies are of crucial relevance to assure the survival and safety of UAVs. Anti-collision of UAVs can be varied in the aspect of the use of sensors and the system’s working principle. This article provides a comprehensive overview of anti-collision technologies for UAVs. It also presents drone safety laws and regulations that prevent a collision at the policy level. The process of anti-collision technologies is studied from three aspects: Obstacle detection, collision prediction, and collision avoidance. A detailed overview and comparison of the methods of each element and an analysis of their advantages and disadvantages have been provided. In addition, the future trends of UAV anti-collision technologies from the viewpoint of fast obstacle detection and wireless networking are presented.

In the majority of children with growth hormone (GH) deficiency (GHD), normal GH secretion may occur before the attainment of final height. The aim of the study was to assess the incidence of persistent and transient GHD and the effectiveness of recombined human GH (rhGH) therapy in children with isolated idiopathic GHD, with respect to the moment of therapy withdrawal and according to different diagnostic criteria of GHD. Analysis included 260 patients (173 boys, 87 girls) with isolated idiopathic GHD who completed rhGH therapy and have reassessed GH and IGF-1 secretion. The incidence of transient GHD with respect to different pre- and post-treatment criteria was compared together with assessment of GH therapy effectiveness. Incidence of transient GHD, even with respect to pediatric criteria was very high. Normal GH secretion occurred before the attainment of near-final height. Application of more restricted criteria decreased the number of children diagnosed with GHD but not the incidence of transient GHD among them. Poor response to GH therapy was observed mainly in patients with normal IGF-1 before treatment, suggesting that diagnosis of GHD may be false positive. Further efforts should be made to avoid overdiagnosis GHD and overtreatment of the patients.

RNA polymerase III (RNAP III) holoenzyme activity and its products processing have been linked to several metabolic dysfunction in lower and in higher eukaryotes. Alterations in the activity of RNAP III driven synthesis of non-coding RNA, causes extensive changes in the glucose metabo-lism. Increased RNAP III activity in S.cerevisiae maf1Δ strain, manifests with lethality when grown on non-fermentable carbon source. This lethal phenotype is suppressed by reducing tRNA syn-thesis. The cause for the lack of growth neither the underlying molecular mechanism has not been deciphered and has been awaiting a scientific explanation for a decade. Our previous proteomics data suggested mitochondrial dysfunction of the strain. Using model mutant strains maf1Δ (with increased tRNA abundance) and rpc128-1007 (with reduced tRNA abundance) we collected data showing major changes in TCA cycle metabolism of the mutants that explain the phenotypic ob-servations. The present study, based on 13C flux data and analysis of TCA enzymes activities, identifies the flux constraints in the mitochondrial metabolic network. The lack of growth is associated with the de-crease in TCA cycle activity and downregulation of the flux towards glutamate, aspartate and phosphoenolopyruvate (PEP), the metabolic intermediate feeding gluconeogenic pathway. rpc128-1007, the strain that is unable to increase tRNA synthesis due to a mutation in C128 subunit, has increased activity of TCA cycle under non-fermentable conditions. To summarize, cells with non-optimal activity of RNAP III, undergo substantial adaptation to a new metabolic state, that make them vulnerable under specific growth conditions. Our results strongly suggest that balanced, non-coding RNA synthesis that is coupled to glucose signaling is a fundamental requirement to sustain cell’s intracellular homeostasis and flexibility under changing growth conditions. Presented results provide insight into the possible role of RNAP III in mito-chondrial metabolism of other cell types.

The experiments described in this research article were designed to test the effect of rare variants into genomic prediction in dairy cattle. Common polymorphisms are able to explain only a small proportion of the underlying genetic variation of complex phenotypes. Variants representing functional mutations with large effects on complex phenotypes are expected to be rare due to natural (humans) or artificial (livestock) selection pressure. Therefore, it is important to check whether the use of rare variants could increase the accuracy of ranking of animals by providing the tool for more precise differentiation among the bulls with high additive genetic merit. The goal of our study was to verify whether including rare variants in a genomic selection model allows for a more accurate description of the additive genetic background of traits under selection in dairy cattle. We used the linear mixed model for comparison SNP estimates for Holstein-Friesian cattle of the two data sets – a set containing only single nucleotide polymorphisms defined by minor allele frequency ≥ 0.01, which is routinely used in the Polish genomic evaluation system (46,216 SNPs), and a set containing SNPs selected based only on the call rate (54,378 SNPs). Based on the SNP estimates we also calculated DGV and GEBV and compared them between both data sets. In all the analyses we used production, fertility, conformation and udder health traits. We also assessed the time required for the two most computationally demanding components of genomic selection: preparing genotype data, and estimation of SNP effects between those two data sets. The results of our study indicated that the analysis including rare variants resulted in changes in the individual ranking of the top 100 male and female candidates, but had no effect on the outcome of the quality of EBV prediction as expressed by the Interbull validation test.

Cardiovascular diseases (CVDs) constitute the first cause of death among the population of developing and developed countries. Atherosclerosis, which is a disorder with multifactorial etiopathogenesis, underlies most CVDs. The available literature includes ample research studies on the influence of classic cardiovascular (CV) risk factors. However, environmental exposure to heavy metals, among other substances, is still an unappreciated risk factor of CVDs. This study aimed to assess the concentration of some heavy metals (copper (Cu), zinc (Zn), manganese (Mn), cobalt (Co), and iron (Fe)) in the blood serum of postmyocardial infarction (post-MI) patients and patients free from myocardial infarction (MI) as well as estimate the relationship between the occurrence of MI and increased concentration of heavy metals. The concentration of heavy metals (Cu, Zn, Mn, Co, and Fe) was assessed using the inductively coupled plasma mass spectrometry technique in a group of 146 respondents divided into two groups: post-MI group (study group (SG), n = 74) and group without cardiovascular event (CVE) having a low CV risk (control group (CG), n = 72). The concentration of the analyzed heavy metals was higher in SG. All the heavy metals showed a significant diagnostic value (p < 0.001). The highest value of area under the curve (AUC) was observed for manganese (Mn) (0.955; 95% confidence interval (CI) = 0.922–0.988), while the lowest value was found for zinc (Zn) (0.691; 95% CI = 0.599–0.782). In one-dimensional models, high concentrations of each of the analyzed heavy metals significantly increased the chances of having MI from 7-fold (Cu) to 128-fold (Mn). All the models containing a particular metal showed a significant and high discrimination value for MI occurrence (AUC 0.72–0.92). Higher concentrations of Cu, Zn, Mn, Co, and Fe were found to considerably increase the chances of having MI. Considering the increasingly higher environmental exposure to heavy metals in recent times, their concentrations can be distinguished as a potential risk factor of CVDs.

Worldwide epidemiological data indicates insufficient diagnosis of diabetes as an increasing problem of public health. Searching for solutions in this disadvantageous situation, occupational medicine health services seem to open up a unique opportunity to recognize some abnormalities in early steps, especially among the asymptomatic working-age population. 316 workers underwent obligatory prophylactic examinations. In patients with twice assayed FGL ≥ 126 mg/dl (7.0 mmol/l) the additional intervention was implemented, including further diagnostic process and therapy in General Practice (GP), followed by examination by an occupational health specialist within 3 months. The diagnosis of previously unknown diabetes was established among 2.5% of examined workers. All patients referred to the GP due to detected glycaemia impairment visited their doctor and finished the diagnostic process, took up therapy constrained by the occupational health physician to show the effects of intervention within 3 months. Prophylactic medical check-ups allow improved compliance and medical surveillance over glycaemia impairment in patients with prediabetes states, unknown diabetes or uncontrolled clinical course of diabetes. Considering fasting glucose level during mandatory prophylactic examination helps effective prevention of diabetes and its complications and thus give public health system benefits.

The main focus of this work is to extend the knowledge about the complexity of issues of oxidative stress. Natural compounds play a serious role in multiple aspects of both human, leading to clear health-promoting effects. This work is focused on the potential application cosmetic formulations containing including but not limited to of clove water and isolated clove oils on their aid in the control of the diseases. The aim of our work was to prepare to evaluate cosmetic formulations containing clove oil, eugenol or new eugenol ester derivative (eugenyl dichloroacetate - EDChA) but also cosmetic formulations containing the aqueous phase obtained after separation of essential oil following the clove buds steam distillation. To evaluate the antioxidants transdermal delivery system, in vitro permeation experiments in a Franz diffusion cell were performed using pig skin. The antioxidative capacity of the cosmetic formulations obtained was determined by the DPPH free radical reduction method. In the next stage, the antioxidant activity (DPPH, ABTS, and Folin–Ciocalteu methods) of the fluid that penetrated through pig skin and of the fluid obtained after skin extraction, were also evaluated. For comparison, studies of cosmetic formulation containing alone dichloroacetic acid (DChAA) were as well carried out. The obtained cosmetic formulations were characterized by of antioxidant activity estimated after 24 hours of conducting the experiment, which indicates long-term protection against reactive oxygen species (ROS) in the deeper layers of the skin. The results of this work contribute to the development of cosmetic formulations with antioxidant potential, emphasizing that the water phases are waste from the process of cloves steam distillation and are not used to prepare cosmetic formulations. The use of waste water from the clove buds steam distillation process is environmentally friendly and not allows us to waste, containing however valuable biologically active compounds (furfural, methyl salicylate, 4-allilofenol, eugenol, α- and β-caryophyllene, eugenyl acetate, β-caryophyllene oxide). Together, these results suggest that cosmetic formulations may be potential drug candidates for chemopreventive, antineoplastic and antimutagenic therapy.

This observational study was ordered by the Medical Practitioners’ Chamber in Warsaw. The objective of the study was to evaluate the health status of physicians in relation to their occupational duties. Professional burnout was considered relative to different features of personality. This study was initially carried out from 2005–2008, but further analysis of burnout and personality was carried out from 2017–2018. The research tools were anonymous, validated questionnaires. The sample size was based on the size of the population— the registry of the Regional Chamber of Medical Practitioners— and literature on burnout prevalence. The respondents’ work places were randomly selected from the Mazovian District register. The test on burnout was completed by 378 respondents, while 62 subjects completed a personality test. Results showed that burnout syndrome was an occupational problem for healthcare workers. Professional burnout affected as many as 42% of respondents(n = 158). It affected two age groups in particular: physicians up to 31 years old and individuals aged 41-50. Moreover, neuroticism was found to be significantly related to burnout syndrome. In conclusion, burnout syndrome is common among medical practitioners, and neuroticism may be correlated with burnout syndrome.

Lymphangioleiomyomatosis (LAM) is characterized by lung cysts that cause lung deterioration, changes in the lymphatic system, and tumors in the kidneys. It mainly affects women of reproductive age and is a progressive disease. LAM can occur as an isolated disease or coexist with tuberous sclerosis (TSC). The source of LAM cells is unknown. Patients with confirmed LAM should be treated with an mTOR inhibitor, sirolimus, or everolimus. We present a case of LAM with TSC in a patient whose symptoms, including lymph nodes and chyaloperitoneum, mainly concern the abdominal cavity.

Fast growing woody crops are currently a very important source for the generation of energy biomass. As short-rotation woody crops, the genus Paulownia has already attracted growing attention. These trees are used to produce biomass and reduce the carbon dioxide concentration in the atmosphere. Most projects for biomass production, however, may affect soil properties and status. For this reason, it is important to know the effects of Paulownia plantations on the microbiological properties of the soils on agricultural areas in Poland. This article provides information on plant parasitic nematodes inhabiting the root zone of Paulownia tomentosa L. in Poland. The only report of Meloidogyne hapla Chitwood, 1949 shows the potential pathogenicity of the root-knot nematodes. Furthermore, nothing is known about plant parasitic nematodes inhabiting the root zone of Paulownia tomentosa L.in Poland. Determining the trophic group of plant parasitic nematodes was undertaken by a process of centrifugation. Measurements showed a decrease in the population reproduction factor (Pf/Pi) which reached a value of 0.1. Paulownia tomentosa L. taken from seven different locations in Poland revealed the presence of M. hapla.

Background: The most common form of vascular access for hemodialysis is a native arteriovenous fistula, which connects site of the artery to the end of the vein. The maturation process of the fistula plays a crucial role in the establishment of a functional vascular access. Radial artery stenosis is among others a potential cause of maturation failure. In these cases, improving the fistula's blood flow may be difficult, as traditional surgical reanastomosis and endovascular intervention frequently fail. Radial artery deviation and reimplantation (RADAR) is a novel, effective technique for creating primary fistulas with a high patency rate. The main disadvantage of this procedure is the ligation of the radial artery and the subsequent known consequences. Methods: In order to accelerate maturation, we used RADAR as a secondary fistula in three patients with radial artery stenosis and maturation failure. In all patients after surgery we observed significant increase in fistula blood flow. Two patients used fistula for hemodialysis after surgery. We describe the image diagnosis, procedure, and benefits of this method based on a single case. Conclusion: The RADAR technique may be successfully used as a secondary access in patients with maturation failure, due to RA stenosis to accelerate fistula maturation.