The tambaqui Colossoma macropomum is the main species of edible fish originating from fish farming in the Amazon region. This study aimed to determine and compare the hematological and biochemical parameters of tambaqui from fish farms in municipalities in the Metropolitan Region of Manaus, Amazonas, Brazil. Two fish farms were sampled in a semi-excavated pond-type breeding system in each of the five towns investigated (Presidente Figueiredo, Manaus, Iranduba, Manacapuru and Novo Airão), totaling ten breeders. On each farm, ten specimens were captured, totaling 100 animals. Blood was collected by caudal puncture, and hematological and biochemical parameters were determined using the methodology described for teleosts fish. For the erythrogram in the variables of hematocrit, hemoglobin concentration and erythrocytes, significant differences were observed in Iranduba and Novo Airão, demonstrating indications of anemic processes and dehydration. For plasma biochemistry, low levels were observed in fish from Iranduba and Novo Airão. High values in lipid compounds were found in tambaqui from Manacapuru. High intraspecific variation was found in the leukogram and thrombogram, which determined similarities between tambaqui from different farms and municipalities. On the other hand, fish from Manacapuru have an inappropriate diet, indicated by the high levels of cholesterol and triglycerides found in the blood.

Tabebuia aurea (Silva Manso) Benth. & Hook. f. The ex S.Moore (yellow ipe), belonging to the Bignoniaceae family, used in the popular for fever, inflammation and healing of skin wounds. The extract was prepared by maceration, using 70% ethanol. Through HPLC analysis, it was possible to identify substances, mainly phenolic, such as lapachol, present in Bignoniaceae. The phenolic content was 21.36 mg / Eag in the antioxidant activity, the effective concentration of 50% was 53.03 ± 1.14 µg / mL. The antimicrobial activity against S.aureus, E. coli and C. albicans was evaluated by microdilution in broth, which verified action against the tested microorganisms. Cell viability has been inhibited for tumor cells, although this has not been observed for normal cells. The LD50 against A.aegypti mosquito larvae was 3504.6 mg / L and there was no mortality in the concentration tested for the snail B.glabrata. Nontoxic or low toxicity for A. salina and T. molitor, respectively, and did not exhibit hemolytic action at concentrations of antibacterial effect. Given the above, it was concluded that the bark extract of the studied species has bioprospecting potential for the future development of antimicrobial products.

The complete regression of clear cell renal cell carcinoma (ccRCC) obtained pre-clinically with anti-carbonic anhydrase IX (CAIX) G36 chimeric antigen receptor (CAR) T cells in doses equivalent to 10e8 CAR T cells/ Kg renewed the potential of this target to treat ccRCC and other tumors in hypoxia. The immune checkpoint blockade (ICB) brought durable clinical responses in advanced ccRCC and other tumors. Here we tested CD8/4-1BB compared to CD28-based anti-CAIX CAR PBMCs cells-releasing anti-programmed cell death ligand-1 (PD-L1) IgG4 for human ccRCC treatment in vitro and in an orthotopic NSG mice model in vivo. Using a 10e7 CAR PBMCs cells/ Kg dose, anti-CAIX CD28 CAR T cells releasing anti-PD-L1 IgG highly decrease both tumor volume and weight in vivo, avoiding the occurrence of metastasis. This antitumoral superiority of CD28-based CAR PBMCs cells compared to 4-1BB occurred under ICB via PD-L1. Furthermore, T cell exhaustion status in peripheral CD4 T cells, additionally to CD8 was critical for CAR T cells efficiency. The lack of hepatotoxicity and nephrotoxicity upon administration of 10e7 CAR PMBCs cells/Kg dose is the basis for carrying out clinical trials using anti-CAIX CD28 CAR PBMCs cells releasing anti-PD-L1 antibodies or anti-CAIX 4-1BB CAR T cells, offering exciting new prospects for the treatment of refractory ccRCC and hypoxic tumors.

Surimi consists in a technique for concentrating myofibrillar proteins with gelling ability, which aims to make use of by-products from fish slaughter, such as bullfrog back, which can be basis for the manufacture of emulsified products. Due to the strategic need to develop clean label foods, products such as collagen have been reported as potential natural additives with bioactive properties. The objective was to elaborate a surimi from the mechanically separated bullfrog back and to develop an emulsion, replacing the soy lecithin used by hydrolyzed collagen, evaluating its emulsifying and antioxidant potential during 120 days of storage under freezing. Three meat emulsions were prepared: EC0 (100% soy lecithin), EC50 (50% soy lecithin + 50% collagen), and EC100 (100% collagen), which were subjected to analysis of chemical composition, emulsion stability, water holding capacity, luminosity, whiteness, texture profile, TBARS and antioxidant activity. Insertion of collagen increased whiteness, lightness, hardness, and chewiness values compared to the EC0, but showed greater fluid loss. All treatments showed stable WHC values (88 – 91%) over time. Replacement with collagen was satisfactory as an emulsifier and as an antioxidant, and the EC50 and EC100 treatments were not negatively affected in the investigated parameters.

Academic publishing is undergoing a highly transformative process, and many rules and value systems that were in place for years are being challenged in unprecedented forms leading to the evolution of novel ways of dealing with new pressures. One of the most important aspects of an integrated and valid academic literature is the ability to screen publications for errors during peer review to weed out mistakes, fraud and inconsistencies, such that the final published product represents a product that has value, intellectually, and otherwise. It is difficult to claim the existence of perfect manuscripts. The level of errors that exist in a manuscript will depend on the rigor of the research group and of the peer review process that was used to screen that paper. When errors slip into a final published paper, either through honest error or misconduct, and are not detected during peer review and editorial screening, but are spotted during post-publication peer review, an opportunity is created to set the record straight, and to correct it. To date, the most common forms of correcting the literature have been errata, corrigenda, expressions of concern, and retractions. Despite this range of corrective measures, which represent artificially created corrals around pockets of imperfect literature, certain cases do not quite fit this mold, and new suggested measures for correcting the literature have been proposed, including manuscript versioning, amendments, partial retractions and retract and replace. In this commentary, a discussion of the evolving correction of the literature is provided, as are perspectives of the risks and benefits of such new measures to improve it.

Climate change in the coming decades could intensify extreme events such as severe droughts. Combined with the possible increase of water demands, these changes exert a large pressure on the water systems. In order to confirm this assumption, a set of scenarios was proposed in this study to consider the combined impact of climate changes and the increase in water demand on the main multiple-use reservoirs of São Francisco River, Brazil. For this purpose, five CMIP6 climate models were used considering two greenhouse gas emissions scenarios: the SSP2-4.5 and SSP5-8.5. The naturalized and withdraw streamflows were estimated to the adopted reser-voirs considering all existing, new and projected demands. The conjunction of scenarios indicat-ed an increase in Potential Evapotranspiration, a possible significant reduction in water availa-bility, a growth in water demand mainly for irrigation and a substantial reduction in the perfor-mance of the evaluated reservoirs.

Objective: To evaluate the direct costs of treating patients with neuromyelitis optica spectrum disorders (NMOSD) in outpatient care, from a hospital and Unified Health System (SUS) perspective. Methods: Observational study with data collection by microfunding, carried out with patients diagnosed with neuromyelitis optics, treated at a university hospital, in the city of Rio de Janeiro. Mann-Whitney tests were performed to measure cost differences between patient groups by disease severity and analysis perspective. Results: Nine individuals with a median age of 48 years (IQR 30 - 59 years) were included. The median total costs for patients with greater severity from the perspective of the SUS and the hospital were R$ 21,638.22 (IIQ: R$ 15,614.63; R$ 25,985.32) and R$ 32,094.11 (IIQ: R$ 25,610 .60; R$ 38,617.25) respectively. For patients with moderate severity the median was R$ 7,339.85 (IIQ: R$ 6,235.45; R$ 11,912.05) from the SUS perspective and R$ 11,505.80 (IIQ: R$ 7,339.85; R$ 22,424.76) from the perspective of the hospital, where the use of medications had a major impact. Conclusion: The main cost drivers were medicines, especially those used off-label, and procedures.

The research reported on this paper was aimed at improving the overall efficiency of a PDO certified artisanal cheese production process. Being a PDO certified foodstuff by the EU, it is considered to have properties and qualities determined by the geographical environment in which is made, with its production taking place in a specific and determined geographical location, in this case the Serra da Estrela region. In that sense, the authors conducted a mapping according to a systemic perspective of the processes involved in the context of manufacturing and distribution of certified Serra da Estrela cheese. Numerous methods were used throughout the process, such as a systemic design analysis, and techniques derived from ethnographic methods, which led to the collection of data in the field and consequently provided the immersion of the researcher in genuine work situations. Critical points were identified and emphasized in the systemic map with the purpose of encouraging initiatives to address and overcome the gaps and inefficiencies detected. The systemic design analysis triggered the development of design work. Observations following an ethnographic approach identified ergonomic risks in cheese making during the process of cutting excess chips, fostering the emergence of musculoskeletal disorders at the wrist. A tool that fits best to the task at hand was developed. A prototype of the new tool enabled collecting feedback from use in the work context, in order to inform product development. The domains of agricultural production and microbiology, considering the specific microorganisms developed trough the ripening process of the cheese, turned out to be aspects of high importance for the issue under focus, contributing to a broader understanding of the ripening process and its risks, simultaneously improving the efficiency and success of cheese production. If it were not for the systemic analysis, which served as a link between the boundaries of distinct domains such as the risk of microorganism contamination, ergonomics, energy efficiency, legislation and regulation policies, transportation challenges and economic viability, approached throughout the research. Simultaneously creating bridges between them, the various problems might not have been detected in the first place, as they are usually addressed in specialized disciplines, predetermined by the restrictions of each specific area of knowledge. As a consequence of the development of this research, which was based on an analysis that sought to establish possible connections between various disciplines and tried to constantly maintain a holistic perspective, making new connections and observing the issues from a new angle, apart from the already established methodologies. All this allowed to lay out the seeds for the development of a plan to tackle the critical points identified by the systemic analysis reported in this paper.

Most research on colostrum quality and colostrum management focuses on maternal IgG transfer to the newborn calf. However, in addition to being a source of antibodies, colostrum has other components that influence passive immune transfer, nutrition, gut maturation, and thermoregulation. In this review, the constituents in the bovine colostrum, as well as their variation, are explored and it is discussed the biological role of colostrum on the newborn calf. Protein is the nutrient in higher concentration and fat is the nutrient with the most variation. There is a pronounced amplitude in the total solids content of colostrum, but the average concentration does not vary much between studies (24.7%). Bovine colostrum major components can vary with several factors, such as the region, season, breed, dry period length, and nutritional aspects of the dam. Bioactive components, cells, and microbiome variability are less understood, besides IgG. Regardless, they play crucial distinct roles in the physiological development of the newborn. However, a multi-interactive effect between these components, cells, and microorganisms is more likely to occur. Recently researched components in colostrum like miRNA, nucleo(t)sides, the milk fat globule membrane, oligosaccharides, minor proteins, and the microbiome are examples of promising interest areas of study.

This review focus on a critical analysis of nanocatalysts for Advanced Reductive Processes (ARP) and Oxidation Processes (AOP) designed for the degradation of poly/perfluoroalkyl substances (PFAS) in water. Ozone, ultraviolet and photocatalyzed ARP and/or AOP will be the basic treatment technologies. Besides the review of the nanomaterials with greater potential as catalyst for advanced processes of PFAS in water, the perspectives for its future development considering sustainability considerations will be discussed. Moreover, a brief analysis of the current state of the art of the ARP and AOP for the treatment of PFAS in water will be presented.

Dengue virus serotype 2 (DENV-2) is responsible for dengue epidemics on a global scale and is recognized for being associated with severe cases of the disease. This study conducted the phylogenetic investigation of DENV-2 in the Northern region of Brazil. DENV-2 isolates from 2017 to 2021, originating from Northern Brazilian states, were selected. Only one lineage (I) of the Asian-American genotype and one lineage (5) of the cosmopolitan genotype were observed. All sequences of the Asian-American genotype used in this study were segregated into a paraphyletic subgroup aligned with sequences from an American clade. These results indicate a probable geographic link starting from Puerto Rico. Additionally, sequences from Acre were identified and grouped into a monophyletic clade of the cosmopolitan genotype. These results indicate the cocirculation of two DENV-2 distinct lineages: Lineage 1, Asian-American genotype (probable origin from Puerto Rico); Lineage 5, cosmopolitan genotype (probable origin from Peru). Our results provide important data regarding the study of DENV genotypes and lineages distribution, as well as opening up possibilities for probable introduction and dissemination routes.

In this paper, we investigate the Green measure for a class of non-Gaussian processes in $\mathbb{R}^{d}$. These measures are associated with the family of generalized grey Brownian motions $B_{\beta,\alpha}$, $0

The topography and chemical composition modification of the titanium (Ti) implants play a decisive role in improving biocompatibility and bioactivity, accelerating osseointegration, and, thus, determining clinical success. In spite of the development of surface modification strategies, bacterial contamination is a common cause of failure. The use of systemic antibiotic therapy does not guarantee action at the contaminated site. In this work, we proposed a surface treatment for Ti implant that aim to improve its osseointegration and reduce bacterial colonization in surgery site due to local release of antibiotic. The Ti disks were hydrothermally treated with 3M NaOH solution to form a nanostructured layer of titanate on the Ti surface. Metronidazole was impregnated on these nanostructured surfaces to allow its local release. The samples were coated with poly(vinyl alcohol) - PVA films with different thickness to evaluate a possible control of drug release. Gamma irradiation was used to crosslink the polymer chains leading to a hydrogel layer formation and to sterilize the samples. The samples were characterized by XRD, SEM, FTIR, contact angle measurements, “in vitro” bioactivity, and drug release analysis. The alkaline hydrothermal treatment successfully produced intertwined, web-like nanostructures on the Ti surface, providing wettability and bioactivity to Ti samples (Ti+TTNT samples). Metronidazole was successfully loaded and released from Ti+TTNT samples coated or not with PVA. Although the polymeric film acted as a physical barrier to drug delivery, all groups reached the minimum inhibitory concentration for anaerobic bacteria. Thus, the surface modification method presented is a potential approach to improve the osseointegration of the Ti implant and to associate local drug delivery to dental implants, preventing early infections and bone failure.

Cardiovascular diseases (CVDs) a leading cause of mortality worldwide. Therefore, there is in-creasing interest in dietary interventions to reduce risk factors associated with these conditions. Cherries and berries are rich sources of bioactive compounds and have attracted attention for their potential cardiovascular benefits. This review summarizes the current research on the effects of cherries and berries consumption on cardiovascular health, including in vivo studies and clinical trials. These red fruits are rich in phenolic compounds, such as anthocyanins and flavonoids, which have multiple bioactive properties. These properties include antioxidant, anti-inflammatory, and vasodilatory effects. Studies suggest that regular consumption of these fruits may reducing in-flammation and oxidative stress, leading to lower blood pressure, improved lipid profiles, and enhanced endothelial function. However, interpreting findings and establishing optimal dosages is challenge due to the variability in fruit composition, processing methods, and study design. Despite these limitations, the evidence highlights the potential of cherries and berries as components of preventive strategies against CVD. Further research is needed to maximize their health benefits and improve clinical practice.

As part of a project ordered by the Ministry of Science, Technology and Innovation of Brazil, an assessment of Brazilian biological collections was conducted by the Brazilian Societies of Botany, Microbiology, Virology and Zoology to obtain data on the current state of these collections. The goal was to provide subsidies for future public policies, including financial support and prioritization policies. Herein, we present the results of the microbiological collections, which play a fundamental role in the ex situ conservation of biodiversity and support research, development and innovation. A survey was sent to public and private institutions in Brazil and answered by 168 microbiological collections from 79 different institutions. Among these, 73 comprise public research institutions and universities, demonstrating the State's importance in preserving and safeguarding Brazilian microbial diversity. The main taxonomic groups are bacteria (present in 70.24% of the collections) and fungi (52.98% of the collections) from different Brazilian ecosystems and biomes, including several type strains. Furthermore, the collections preserve microorganisms with biotechnological potential for application in environmental protection, public health, industry, and agribusiness. However, despite all these economic and biotechnological potentials, the data analysis showed serious limitations and fragilities, especially regarding physical infrastructure and human resources, and raises alerts about the risk that Brazilian collections are subjected to.

This brief report presents a comparative analysis to examine differences in the mean dose of nitrate supplementation between studies with positive and negative results on exercise performance. Protocols of at least six days that had positive results used lower nitrate doses (~7.7 mmol), whereas those with negative results used higher doses (~10.2 mmol), showing a statistically significant difference (p < 0.05). These findings suggest long-term supplementation with higher doses (~10.2 mmol) may not improve exercise performance. Further research is essential to understand the underlying mechanisms.

Contrary to previous belief, mutation frequency also depends on how essential a gene is. Mutation frequency is substantially lower in the coding regions of essential genes and higher in less essential genes. Even though the mutation bias based on gene essentiality is incapable of invalidating Darwinism, this bias certainly added a rather unsubtle obstacle to it by contradicting a paradigm that was widely held: that mutations are random in the sense of being independent of their function for the organism. The reason this bias occurs is currently unknown. Here, I present the theoretical solution of why genetic mutations also depend on whether the gene is more or less essential. My theory is that each gene or epigene has its own level of resistance to entropic elevation, something directly proportional to the frequency of its expression. The more a gene is expressed, the higher is its resistance to entropic elevation and vice versa. For genetic information to be conserved instead of suffering mutation, a relevant factor is also the frequency of its expression. The energy expenditure directed to a gene of current utility engenders resistance to entropic elevation of the information encoded in that gene, as energy enables not only the existence of information but also its conservation. My theory—which is based on fundamental physics and contains predictions that can be empirically tested—solves a crucial problem in evolutionary biology and genetics; if I am right, I will have established a biological law. Finally, my aim here is also to provide a substantial advance in knowledge about the relationship between information, genetics, and thermodynamics.

Green carbon-based materials (GCM), i.e. carbon materials produced using renewable biomass or recycled wastes, ought to be used in order to processes become sustainable and carbon neutral. Carbon nanomaterials, like for example carbon dots and nanobichar families, and carbon materials, like for example activated carbon and biochar substances, are sustainable materials with great potential to be used in different technology applications. In this review, the following four applications were selected, and the works published in the last two years (since 2022) critically reviewed: agriculture; water treatment; energy management; and, carbon dioxide reduction and sequestration. GCM improved the performance of the technological applications under revision and play an important role in the sustainability of the processes, contributing to the mitigation of the climate change, namely by reducing emission and increase sequestration of CO2eq..

A sustainable matrix based on eucalyptol essential oil/sawdust was developed and applied on one-sided laminated plywood. This finish aims to serve as a eucalyptol odor slow release. Eucalyptol odor release was monitored with gas chromatography coupled with a flame ionization detector (GC – FID: Limits of Detection and Quantification of 0.70 g/m3 and 2.11 g/m3, respectively, and with linearity up to 18.6 g/m3). Measurement of the eucalyptol odor released was performed during a six-month period and it was found that the release followed a first-order exponential decay with a decay rate constant of 0.0169 per day. The half-life was determined to be of 48 days. The granulometry and particle size porosity of sawdust were analyzed by Scanning Electron Microscopy. Sawdust size fraction between 112 – 200 μm showed best eucalyptol absorption capacity with 1:3 masses ratio (sawdust:eucalyptol). The time capacity of eucalyptol release depends of composite eucalyptol – sawdust quantity. Where this relation was determined: 15.0 grams of composite eucalyptol-sawdust by 0.8 mm diameter aperture gave 6 months of eucalyptol release, that was considered very positive, understanding high volatility of eucalyptol and a small quantity of composite for further products. The new product is characterized by a carbon footprint (considering the industry frontiers) of 5.94 kg CO2eq/m2 of plywood floor.

Fungal infections caused by Candida spp. are responsible for high hospital morbidity and mortality rates. Therefore, there is a continuous search for new antifungal medicines, particularly ones with anti-Candida activity. This review analyzed articles published between 2002 and 2023 considering the anti-Candida activity of chemical compounds identified or isolated in plants of the Anacardiaceae family. In addition, the in-silico prediction of the isolated compounds described was performed. The analysis of 35 studies showed that extracts, essential oils, and compounds from the anti-Candida activity were frequently determined in vitro using the minimum inhibitory concentration (MIC). The most commonly tested species were Candida albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, and C. guilliermondii, respectively. Essential oils were the most used form (37% of the studies). The isolated compounds with antifungal effects include cardanol, estragole, trans-anethole, β-caryophyllene, myrcene, catechin-3-o-rhamnoside, β-sitosterol-3-O-glucoside, 24Z-isomasticadienolic acid, oleanolic acid, and oleanolic aldehyde. The in-silico evaluation of those isolated compounds revealed the compounds' drug-likeness and possible antifungal activity. However, some of them showed high toxicity. In conclusion, compounds from the Anacardiaceae family show promise for developing new therapeutic antifungal drugs, especially in combination with conventional antifungals.

Introduction of exotic species in new areas through anthropic action is one of the major problems that can affect biodiversity. Branchiomma luctuosum is known for its highly invasive potential and the actual occurrence of species commonly associated with port activity areas is an extra evidence that this anthropogenic activity should not be underestimated. In order to develop suitable molecular markers for future studies on colonization routes and population dynamics of the invading individuals of B. luctuosum, nine highly polymorphic microsatellite loci were isolated and their polymorphism levels were evaluated. These loci showed a range of number of alleles per locus from five to ten and all loci had a high level of genetic diversity, and exhibited significant heterozygote deficiencies probably due to the presence of null alleles. Significant deviations from the Hardy-Weinberg equilibrium were detected at several loci and most of them were related to a heterozygous deficit. Heterozygous deficiency can be expected in this case due to the biology and history of this invasive species, in relation to its recent introduction in Brazilian coast and possible action of multiple introductory events.

This work proposes a new theoretical approach for the next generation of extremely efficient motors, whose impact will be substantial for future sustainable technological development. Building on well-known physical concepts, this approach introduces the concept of equilibrium of mutual retention, upon which a device remains in an equilibrium state between movement and attraction-repulsion. This new theoretical approach showed promising results in computer simulation experiments, indicating that the equilibrium of mutual retention can allow for a new way of modeling an extremely efficient motor. Finally, a theoretical efficiency analysis showed the need to expand the limits of some physical concepts already established.

In this work, we present a model of the atom that is based on a nonclassical logic called paraconsistent logic (PL), which has the main property of accepting the contradiction in logical interpretations without the conclusions being annulled. The proposed model is constructed with an extension of PL called paraconsistent annotated logic with annotation of two values (PAL2v), which is associated with an interlaced bilattice of four vertices. We use the logarithmic function of the Shannon entropy H_(s) to construct the paraconsistent equations and thus adapt a probabilistic model for representations in quantum physics. Through analyses of the interlaced bilattice, comparative values are obtained for some of the phenomena and effects of quantum mechanics, such as superposition of states, quantum entanglement, wave functions, and equations that determine the energy levels of the layers of an atom. At the end of this article, we use the hydrogen atom as a basis of the representation of the PAL2v model, where the values of the energy levels in six orbital layers are obtained. As an example, we present a possible method of applying the PAL2v model to the use of Raman spectroscopy signals in the detection of lubricating mineral oil quality.

The development of electric aircrafts is becoming an important technology for achieving the goals set by the European Commission for the reduction of gases emissions by 2050 in the aeronautical transportation system. However, there is a gap between the values of specific power in commercial electric machines and the ones required for aeronautical applications. Therefore, the search for alternative materials and non-conventional designs is mandatory. One emergent solution is using superconducting machines and systems to overcome the current limits of conventional electrical machines. This work reviews the new hybrid and all-electric aircraft tendencies, complementing it with recent research on the design and development of high specific power superconducting machines. This includes the main topologies for hybrid and all-electric aircraft, with an overview of the ongoing worldwide projects of these types of aircrafts, systematizing the main characteristics of their propulsion systems. It also includes the research on superconducting machines for the purpose of high specific power, considering the impact on the redesign of aircraft systems in the electrical, cooling, and fuel source sense.

Medicinal plants for bone grafts are promising because they are free from infecting microorganisms and biocompatible. We report a novel study evaluating osteoregeneration of bone grafts from extracts of Alternanthera brasiliana and Fridericia platyphylla after bone injury induced by radius fracture in rats. Grafts were obtained using 2% chitosan gel and 0.5% hydroalcoholic extract. The rats were randomly divided into four experimental groups (N= 12): Negative control (NC) - Chitosan gel; Positive control (PC) - bovine mineral bone graft (Lumina Bone®, fine powder 0.5); F. platyphylla Graft (FRID) - 0.5% bone graft; A. brasiliana Graft (ABRA) - 0.5% bone graft. The animals were evaluated for three periods, 30, 60, and 90 days after fracture induction. Bone alkaline phosphatase (BSAP) and radiographic and histological evaluations were followed. After 90 days, there was an increase in BSAP for the ABRA group on the newly formed bone matrix but not for FRID, indicating the presence of active osteoblasts. Additionally, mature bone tissue and bone remodeling were observed, and a conspicuous presence of type I collagen for both FRID and ABRA. We demonstrated that FRID and ABRA grafts produced early bone neoformation. New perspectives for these species as a graft are suggested.

Background: Hospital accreditation has become ubiquitous in developing countries. Although studies recognize that accreditation can improve healthcare quality, efficiency, and safety, there are doubts about how hospitals deal with conflicts caused by the different institutional logics that inhabit this process. Therefore, this study aimed to investigate how professional and market logics, as well as the conflict between institutional demands, affect compliance with hospital accreditation. Methods: To this end, we developed a multiple case study in four Brazilian hospitals through in-depth interviews with sixteen participants (managers, physicians, nurses, physiotherapist) and on-site observation by the triangulation between the analysis of the narratives and the results of the multiple correspondence analysis. The interpretation and subsequent categorization of the interviews were guided by the study's analytical categories: institutional logics (professional and market); adoption objectives (legitimacy and efficiency); strategic responses to adoption (conformity, non-conformity, and customization); nature of demands (origin in the means and in goal). Results. The data showed that when professional logic is prominent, there is a greater tendency to customize activities, as there are conflicts in the means by which activities can be developed. When market logic stands out, there is a risk of non-conformity, mainly because the focus falls exclusively on goals. Finally, the data point to the absence of conflicts between justifications related to efficiency and legitimacy. Conclusion. We conclude the study by highlighting the theoretical and practical implications of recognizing the conflict between logics, contributing to a deeper understanding of how logic influences the attention given to specific demands and, more significantly, how they affect compliance with hospital accreditation standards, expanding the body of knowledge in the area.

The construction industry, a vital sector of the global economy, is projected to grow significantly over the next 15 years. Despite its importance, this industry struggles with inefficiency, high costs, prolonged timelines, and substantial ecological impacts. These challenges have led governments to advocate for Building Information Modeling (BIM) to enhance Efficiency and sustainability through comprehensive digital representations of physical and functional characteristics. The effective integration of BIM technology hinges on the active participation of industry providers, raising the question of how these providers can positively contribute to improving BIM dimen-sions. This study investigates the potential of coopetition networks within the Portuguese Orna-mental Stone industries (OS-PT) to address this challenge. Coopetition networks, which blend competition and cooperation, foster collaborative networks among companies to enhance their re-sponse to digital supply chains. The research employs an Experimental Pilot Network facilitated by an Industrial Internet of Things (IIoT) artefact to assess the benefits of coopetition networks. The findings demonstrate that transitioning from current practices (CB.P) to coopetition network practices (CN.P) significantly improves operational Efficiency, labour productivity, and sustaina-bility. Specifically, the transition resulted in a 15.6% increase in on-time delivery performance, a 27.38% improvement in labour productivity, and a 21.8% reduction in CO₂ emissions per part produced. These improvements align with Sustainable Development Goals (SDGs) 8, 9, 11, and 13, highlighting the strategic value of coopetition in driving innovation and sustainability in the construction industry. Future research should explore the scalability of these findings across different sectors and further refine methodologies to maximize the benefits of coopetition net-works.

Microencapsulation by atomization can be used to minimize the loss of bioactive compounds, improving the characteristics and applicability of the powdered product. This study evaluates the effect of different encapsulating agents (maltodextrin, gum arabic, and chitosan) and three spray nozzle diameters (1.0, 1.2, and 1.5 mm) on the atomization of guavira (Campomanesia adamantium) pulp with added 6% bocaiuva (Acrocomia aculeata) dehydrated pulp in the green maturation stage. The experiment followed a simplex-centroid mixed design, with carrier agent concentrations and spray nozzle diameters as independent variables and powder yield, solubility, hygroscopicity, and vitamin C content as dependent variables. The results show that gum arabica increased powder yield and vitamin C retention. The addition of maltodextrin resulted in powders with increased solubility and low hygroscopicity. Furthermore, the optimization tool indicated that the best process conditions were achieved with the addition of gum arabic (10.8%) and chitosan (7.2%), resulting in powders with 29.48% yield, 82.96% solubility, 15.90% hygroscopicity and 741.11 mg/100g vitamin C. Furthermore, the 1.0 mm diameter spray nozzle was considered the most suitable for obtaining guavira pulp microparticles with higher ascorbic acid content.

The N-acylhydrazone function has been reported as a pharmacophore group of molecules with diverse pharmacological activities, including anti-inflammatory effects. Thus, this study evaluated the anti-inflammatory potential of the compound N'-(3-(1H-indol-3-yl)benzylidene)-2-cyanoacetohydrazide (JR19) in vivo. The study started with the carrageenan-induced peritonitis model, followed by an investigation of leukocyte migration using the subcutaneous air pouch test and an assessment of the antinociceptive profile using formalin-induced pain. To find out the likely mechanism of action, a preliminary molecular docking study was performed focusing on the crystallographic structures of NFκB, iNOS, and sGCs. The computational study revealed satisfactory interaction energies with the selected targets, and the same peritonitis model was used to validate the involvement of the nitric oxide pathway and cytokine expression in the peritoneal exudate of mice pretreated with L-NAME or methylene blue. In the peritonitis assay, JR19 (10 and 20 mg/kg) reduced leukocyte migration by 59% and 52%, respectively, compared to the control group, with the 10 mg/kg dose used in subsequent assays. In the subcutaneous air pouch assay, the reduction in cell migration was 66%, and the response to intraplantar formalin was reduced by 39%, particularly during the inflammatory phase, suggesting that the compound lacks central analgesic activity. In addition, a reversal of the anti-inflammatory effect was observed in mice pretreated with L-NAME or methylene blue, indicating the involvement of iNOS and sGC in the anti-inflammatory response of JR19. The compound effectively and significantly decreased the levels of IL-6, TNF-α, IL-17, and IFN-γ, and this effect was reversed in animals pretreated with L-NAME, supporting a NO-dependent anti-inflammatory effect. In contrast, pretreatment with methylene blue only reversed the reduction in TNF-α levels. Therefore, these results demonstrate the pharmacological potential of the novel N-acylhydrazone derivative, which acts through the nitric oxide pathway and cytokine signaling, making it a strong candidate as an anti-inflammatory and immunomodulatory agent.

This study evaluated the hip joints of the giant anteater (Myrmecophaga tridactyla) through imaging and macroscopic analyses and proposed a surgical approach for arthrotomy procedures in this species. The study used four samples fixed in 10% formaldehyde and six thawed carcasses. Radiographic and ultrasound examinations and anatomical dissection allowed the proposal of a surgical approach for the hip joint. The radiographic evaluation occurred with frog-legged ventrodorsal and lateral projections, and the ultrasound enabled joint soft tissue assessment. The articular elements represented similar characteristics to other domestic species. Some anatomical particularities of muscle groups were the intermuscular septum between the tensor fasciae latae and superficial gluteus muscles at the level of the greater trochanter of the femur, which provides lateral access to the femoral head and neck more conservatively. In conclusion, the anatomical particularities of radiographic positioning, the clinical applications of joint ultrasound, and the arrangement of muscle groups must be considered when evaluating and surgically accessing the hip joint of the giant anteater.

The objective of this review is to do an overview about the current knowledge of Anti Iglon5 Syndrome, a disease that was first described in 2014. The IgLON proteins are a family of cell adhesion molecules and the presence of antibodies against IgLON5 are crucial for diagnosis of Anti IgLON5 Syndrome. This syndrome has an expanded clinical spectrum that involves prominent sleep disorder, progressive bulbar dysfunction, gait instability with abnormal eye movements reminiscent and cognitive deterioration sometimes associated with chorea. The main neuropathological finding is the neuronal loss with hyperphosphorylated tau (p-Tau) protein accumulation at hypothalamus, brainstem tegmentum, hippocampus, periaqueductal gray matter, medulla oblongata and upper cervical cord. The exact pathogenesis is still unclear and involves a neurodegenerative process and autoimmune response. The early diagnosis is important to avoid unnecessary tests and prevent complications. Important resources for diagnosis are the antibody testing of serum and CSF for IgLON5-IgG. The mortality of anti IgLON5 syndrome is high and new studies published described a good response to immune therapy. However, the response to immune therapy depends of some clinical and analytical characteristic. In addition, future studies are needed to thoroughly study the aspects of pathogenesis and treatment of this important pathological syndrome.

The Gram-negative, strictly anaerobic bacterium Megasphaera elsdenii was first isolated from the rumen in 1953 and is common in the mammalian gastrointestinal tract. Its ability to use either lactate or glucose as its major energy source for growth has been well documented, although it can also ferment amino acids to ammonia and branched-chain fatty acids, which are growth factors for other bacteria. The ruminal abundance of M. elsdenii usually increases in animals fed grain-based diets due to its ability to use lactate (the product of rapid ruminal sugar fermentation), especially at low ruminal pH (&lt; 5.5). M. elsdenii has been proposed as a potential dietary probiotic to prevent ruminal acidosis in feedlot cattle and high-producing dairy cows. However, this bacterium has also been associated with milk fat depression (MFD) in dairy cows, although proving a causative role has remained elusive. This review summarizes the unique physiology of this intriguing bacterium, and its role of in ruminal community function and in the health and productivity of the host animal. In addition to its effects in the rumen, the ability of M. elsdenii to produce C2-C7 carboxylic acids – potential precursors for industrial fuel and chemical production, is examined.

Since its inception in December 2019, Covid-19 has challenged the global scientific community. Some treatments were used in this infection, but doubts still persist regarding the use of medications ¹. More severe cases complicate with endothelial dysfunction, excess thrombin synthesis and decreased fibrinolysis which, associated with hypoxemia, lead to a hypercoagulability state ² and some authors indicate the use of anticoagulants for these ³. For mild cases, doubts remain regarding this indication. We will report the case of a patient with mild symptoms of covid-19, complicated by inferior mesenteric vein thrombosis.

The required processes and steps for making bread include technological and innovative concepts. The current trend is the use of less toxic compounds and green processes. Besides lactic acid bacteria and yeast, other microorganisms with special properties, such as enzymes, new aroma and flavor, exopolysaccharides, and vitamins, among other compounds with beneficial properties, could be added to bread manufacture, improving bread quality and health effects for the consumers. Freeze drying, new encapsulation methods, cryoprotectants, spray drying, fluidized bed drying, and vacuum drying are used for probiotics and microorganism cultures that will be used as starters or biological additives in the fermentation. The same development is observed in the preservation methods, and studies with plant extracts and essential oils have been proposed and introduced, replacing chemical agents, such as propionate, within the clean-label bread formulations concept. Baking science is a growing research line incorporating innovative methods and biological additives.

The antioxidant activity of molecules constitutes an important factor for the regulation of redox homeostasis and reduction of oxidative stress. Cells affected by oxidative stress can undergo genetic alteration, causing structural changes and promoting the onset of chronic diseases, such as cancer. The in silico study performed here was developed to evaluate the antioxidant potential of two molecules, ZINC08706191 (Z91) and ZINC08992920 (Z20), with recognized epithelial anticancer potential. Molecular docking, chemical-quantum calculations and Pearson's correlation were performed. The Z91 and Z20 molecules showed lower binding free energy (ΔG) values for the receptor-ligand interaction than the reference molecules (caffeine – CAF and ascorbic acid – AA), and better results for values of molecular descriptors correlated with ΔG, resulting in a decrease of ΔG. Strong correlations were observed between ΔG values for the five receptors evaluated and ΔG values of the potential epithelial anticancer activity available in literature. These results attest to the significant antioxidant potential of the Z91 and Z20 molecules and their strong relation with the potential epithelial anticancer activity and may be indicated for further analysis in relation to the control of oxidative stress and epithelial anticancer activity.

In order to monitor the diversity of terrestrial biomes, the Earth Exploration Satellite Services demand a high data rate for downlink transmissions while sharing their frequency spectrum with, and suffering interferences from, cellular Base Stations. Both highlight the dilemmas of spectral efficiency (bps/Hz) and spectral coexistence. Our research aims to mitigate interference on low orbit satellite downlinks using Cognitive Radio (CR) and Adaptive MODulation and CODing (MODCOD) techniques. To fulfill this: 1) we present CR approaches to enhance spectrum exploitation. Next, 2) we detail Adaptive MODCOD (ACM) technique to increase RF power and spectral efficiencies. Then, 3) we suggest the solution by combining 1) and 2). Afterwards, 4) we analyze it by monitoring the signal to interference plus noise ratio and the CR/MODCOD strategy. Finally, 5) we provide case studies to demonstrate the strategy’s effectiveness through the practical operation of satellite services, and 6) we conduct a bench test of the communication system.

Invasive macrophytes are considered problematic in natural environments and hydroelectric reservoirs. Climate changes, the occurrence of watercourses, and biotic interactions influence the biological invasion of macrophytes. Native species can correlate with invasives positively or negatively. Urochloa subquadripara is an invasive in natural or disturbed habitats co-occurring with the natives Eichhornia crassipes and Salvinia minima. Aquatic plant communities can be altered by climate change, so species distribution models (SDMs) are important tools for predicting in-vaded areas. This work aimed to make an SDM for U. subquadripara correlating with the poten-tial distribution of native species E. crassipes and S. minima. Occurrence data for U. subquadripara, E. crassipes, and S. minima were collected from databases and in consultation with the published literature. Parameters encompassing biological information of the species were entered into the CLIMEX software and used to generate the Ecoclimatic Index (EI). The species co-occurrence was performed based on multicriteria decision-making (MCDM), and weights were assigned using the analytical hierarchy process (AHP). It was observed that U. subquadripara, E. crassipes, and S. minima have a higher occurrence in tropical and subtropical regions; however, it is predicted that, from climatic changes, these species may move to high latitudes. According to climate change, the risk of invasion by U. subquadripara in the northern hemisphere is mainly in lakes, while in the southern hemisphere, the areas conducive to invasions are rivers and reservoirs. In general, emerging and floating macrophyte species such as U. subquadripara, E. crassipes, and S. minima will be favored, causing suppression of submerged species. Therefore, identifying the potential distribution of these species allows the creation of pre-invasion intervention strategies.

The relationship between perception of anxiety and quality of life in caregivers of children with autism was verified. To verify quality of life, the Short Form Healthy Survey Item was used; perception of anxiety was assessed using the Beck Anxiety Inventory. 80 caregivers of children with autism participated, with 68.7% of caregivers being mothers. Of the total number of caregivers, 68.8% had a severe level of anxiety. Regarding quality of life, they demonstrated greater impairment in Limitation due to physical aspects, Limitation due to emotional aspects, Vitality and Pain. Caregivers with severe anxiety levels had worse quality of life in the domains of pain (p = 0.012), social aspects (p =&lt; 0.001), limitation due to emotional aspects (p = 0.001) and mental health (p =&lt; 0.001). However, in the functional capacity domain, caregivers with a moderate level of anxiety had a better physical capacity score (p = 0.001). There was a negative correlation between the general anxiety score and the general physical (p = 0.029) and general emotional component of quality of life (p =&lt; 0.001). It was found that caregivers of children with ASD present losses related to psychological factors, quality of life and the lack of social support.

Sleep is of vital necessity for health, it has a restorative and protective function for children and adolescents with chronic conditions and their families. The purpose was to identify the scientific production on sleep patterns in children and adolescents with chronic conditions and their families. This integrative review was developed in March and June 2022, of the MEDLINE, Web of Science, CINAHL and PSYCInfo. The articles included were original papers published be-tween January 2007 and mid-2022. Excluded were review studies that did not evaluate sleep and whose participants did not have chronic conditions or were not children, adolescents and/or their families. The searches returned 814 abstracts. After exclusions, 47 studies were selected to be read in full; of these, 29 were selected and were grouped empirically into four categories: major alterations in sleep patterns of children and adolescents with chronic conditions; the relationship between sleep disorders and symptoms in children and adolescents with chronic conditions; impaired sleep patterns of families of children and adolescents with chronic conditions; sleep alterations and their relationship with other problems in families of children and adolescents with chronic conditions. All studies showed sleep pattern impairment in children and adolescents with chronic conditions and their families.

Atrial fibrillation (AF) is a cardiac arrhythmia caused by electrophysiological anomalies in the atrial tissue, tissue degradation, structural abnormalities, and comorbidities. A direct relation exists between AF and altered mitochondrial activity resulting from membrane potential loss, contractile dysfunction, or decreased ATP levels. This review aimed to elucidate the role of mitochondrial oxidative mechanisms in AF pathophysiology, the impact of mitochondrial oxidative stress on AF initiation and perpetuation, and current therapies. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Extension for Scoping Reviews. PubMed, Excerpta Medica Database, and Scopus were explored until June 2023 using "MESH terms." Bibliographic references from relevant papers were also included. Oxidative stress is an imbalance that causes cellular damage from excessive oxidation, resulting in conditions such as AF. An imbalance in reactive oxygen species production and elimination can cause mitochondrial damage, cellular apoptosis, and cardiovascular diseases. Oxidative stress and inflammation are intrinsically linked, and inflammatory pathways are highly correlated with the occurrence of AF. AF is an intricate cardiac condition that requires innovative therapeutic approaches. The involvement of mitochondrial oxidative stress in the pathophysiology of AF introduces novel strategies for clinical treatment.

The orchid genus Brachystele Schltr. comprises 20 species distributed from Mexico to Argentina; 10 are found in Brazil. Anatomical studies of Orchidoideae Lindl. have been scarce, and the anatomy and histochemistry of Brachystele are still largely unknown. We characterized the vegetative organs of B. guayanensis (Lindl.) Schltr. using standard anatomical and histochemical microtechniques. Brachystele guayanensis was observed to display the anatomical characters commonly found in the vegetative organs of representatives of the Orchidaceae and Orchidoideae (including a uniseriate epidermis, thin cuticle, amphistomatic leaves, anomocytic, diacytic and tetracytic stomata, a homogeneous mesophyll, collateral vascular bundles, rhizomes with pericyclic fibers, roots with velamen, uniseriate exodermis, endodermis and pericycle). Histochemical tests confirmed the presence of lignin, proteins, and alkaloids, the lipidic nature of the cuticle, starch grains stored in spiranthosomes in the roots, and the composition of the raphides. Alkaloids were observed in great abundance, especially in the roots, and may have potentially useful medicinal activities, as has been observed in groups phylogenetically related to Brachystele.

Despite the global richness of food plants, only a small portion is used as food for humans. Although most wild food plants (WFPs) are underutilized as food, they can contribute to food security and biocultural conservation through sustainable management. The goal of this research was to identify the best terminological presentation of a novel food product and to understand the influences of the expectations on products with WFP. This research was conducted using an online questionnaire with a final national sample of 724 people. The participants answered questions about their socioeconomic profile, food neophobia, prior knowledge, and expectations regarding the taste and appropriateness of native fruit juices. The questions were randomized so that a person would not receive the same picture or product name more than once. The results show that taste expectancy decreases in relation to products associated with WFP names and forest environments. Food neophobia is the variable that influences expectations the most. To ensure biocultural conservation and cultural valuation, WFP popularization programs should maintain the original (popular) name of the species. However, to help more distant consumers develop familiarity, the flavor, shape, color, or texture of these products can be associated with those of conventional plants.

In this work, we present some results related with the issue of the Loop Quantum Black Holes (LQBH) thermodynamics by the use of the tunneling radiation formalism. The information loss paradox is also discussed in this context, where we have considered the influence of back reaction effects.

The electronic transport stability in nanodevices composed by metal/trans-polyacetylene /metal with different long length has contributed greatly for performance, homogeneity, stability, organization of the chains, reproducibility and higher conductivity. In this paper, we present an analytical study of the electronic transport characteristics from dimerized trans-polyacetylene (trans-PA) molecules containing an odd-even number of sites coupled to metal leads (left and right) in T-shaped geometry using the extended Su-Schrieffer-Heeger (SSH) model based on tight-binding Hamiltonian with the Non-Equilibrium Green´s Function (NEGF) via Heisenberg´s equation of motion and the Keldysh´s formalism. Due to the complexity of the T-shaped odd-even chain, our proposal was to test the effects on the finite-length network for three, four, five sites and furthermore foresee for 17-sites. We show how to tune dimerization strength () coupling to the parameters and T-shaped geometry of the device to which it affects the overlap integral localized at the three endpoints of the T-shaped system, making both the odd and even chains to undergo a metal-insulator transition in their electronic behavior. The results reached through control parity of the chain plane of the parameters governing () the electronic and experimental tunneling allow a better understanding of the subject.

In the semi-arid region of Brazil, it is common to use saline or brackish water for crop production. However, this practice can negatively affect the soil, nutrient balance, and fruit quality and production. From this perspective, this study aimed to evaluate the effect of fertilization combinations with nitrogen (N), phosphorus (P), and potassium (K) on the production and quality of West Indian cherry grown under salt stress in the second year of production. The study was conducted in a protected environment following a randomized block design with treatments distributed in a 2 x 10 factorial arrangement referring to two levels of electrical conductivity of irrigation water (ECw) (0.6 and 4.0 dS m-1) and 10 NPK fertilization combinations (80-100-100; 100-100-100; 120-100-100; 140-100-100; 100-80-100; 100-120-100; 100-140-100; 100-100-80; 100-100-120 e 100-100-140% of the recommendation in the second year of production), with three replicates and one plant per lysimeter. The results indicate that irrigation of West Indian cherry crop irrigation at the water salinity of 4.0 dS m-1 negatively affected all fruit production variables. The interaction between the ECw of 0.6 dS m-1 and the 100-80-120 NPK fertilization combination increased the total number of fruits and the total fruit weight of West Indian cherry.

In the present paper four passive micromixers designs (G1, G2, G3 and G4) inspired on distillation columns trays were proposed. The devices performance was assessed by numerical simulations. The mixing performance was investigated for a Reynolds number range from 0.01 to 100 and channel height of 200 µm, 500 µm and 1000 µm for oil/ethanol flow. G1 and G4 designs provided high mixing index (> 0.975). The G1 device achieved superior mixing performance with a moderate pressure drop (about 0.5 MPa) due to the induced flow recirculation pattern for a relative high flow rate of 0.21 L/min, highlighting the potential use of such microdevice for scale-up and numbering-up of microdevices in modular chemical plant processing.

Volumetric models are a crucial tool for forest management. The study aimed to adjust the mixed model by Schumacher and Hall for estimating commercial volume in a dense ombrophilous forest situated in Ferreira Gomes, Amapá, Brazil. Cubage data from 100 sample trees of 5 species were used to adjust the Schumacher and Hall model in fixed and mixed non-linear forms with the diameter class as a random effect. In addition, the same model was also adjusted for each class to verify whether good equations will be generated by stratifying by diameter class. After analysis, it was found that most of the equations by diameter class do not present all significant parameters. On the other hand, the mixed models using class as a random effect showed good results. Finally, it can be seen that mixed models are a much better alternative than stratification by diameter class for volume estimation.

Let G be a connected Lie group with Lie algebra g. This review is devoted to studying the fundamental dynamic properties of elements in the normalizer NG of G. Through an algebraic characterization of NG we analyze the different dynamics inside the normalizer. NG contains the well-known left-invariant vector fields and the linear and affine vector fields on G. In any case, we show the shape of the solutions of these ordinary differential equations on G. We give examples in low-dimensional Lie groups. It is worth saying that these dynamics generate the linear and bilinear control systems on Euclidean spaces and the invariant and linear control systems on Lie groups. Moreover, the Jouan Equivalence Theorem shows how to extend this theory to control systems on manifolds.

The Sustainable Development Goals (SDGs) raise quality employability, gender equity in access to employment and increase coverage in education, however, in Colombia, high unemployment rates and informality of young people are risks of fulfilling these objectives. This is verified by a study with cohorts on access to employment, labor mobility and entry to quality occupations, through the use of probabilistic models. This study found that young people are less likely to be employed than adults, education has increased in the new generations and allowing them to enter a higher quality occupation, job segmentation and lack of experience of young people are the main cause of unemployment and Women are less likely to be employed than men and to do so in quality jobs. To comply with the SDGs, you must increase coverage in education, make a differential gender policy and expand programs as learners and insert dual education.

This study focuses on the Cereja River, a Permanent Preservation Area located in Amazon coast. The hypothesis tested was that the water quality of the Cereja River has worsened over recent years due to unplanned urban growth. To assess changes in the water quality, seven campaigns were undertaken with data being collected at six fixed points, during two distinct periods (2013–2014 and 2018–2019). High-resolution satellite images from GoogleEarth (2012 and 2019) were used to register unplanned occupation. The DPSWR (Driver-Pressure-State-Welfare-Response) socioecological framework was used to identify potential measures for the management of environmental and anthropogenic problems. The increase in the number of households found on the Cereja margins between 2012-2019 has contributed to the river’s intense trophic condition, low concentrations of dissolved oxygen, high concentrations of pathogenic bacteria, and loss of vegetation cover. The water of the Cereja River is inadequate for any human use, and its contamination is one of the principal factors determining the reduction of the water quality of the Caeté estuary. According to the obtained results, the DPSWR model proved to be an excellent analytical tool for the evaluation of specific local scenarios, through the integrated analysis of socio-environmental issues. The scenario observed can be extrapolated to other rivers located within urban areas in the Amazon region that have relatively unplanned growth.

The main objective of this review is to determine the main risks that agricultural workers are exposed to during pesticide application, which may have a harmful effect on their health and on public health. This systematic review was based on the PRISMA guidelines. A search for articles was conducted in the Medline/PubMed, ScienceDirect, and Web of Science databases. Fifteen articles were selected considering their assessment of agricultural workers' knowledge, perceptions, attitudes, practices, and behaviours, identifying the main risks and risk factors for disease associated with the unsafe handling of pesticides. The main risk factors identified were age, education, pesticide safety training, farming experience, and contact with other farmers/intermediaries resulting in pesticide access. The most frequent risk behaviours were: application of pesticides without personal protective equipment (PPE), incorrect disposal of empty packaging and waste, and undervaluation of label information, as well as other unsafe practices. A multidisciplinary and more effective training must be delivered in order to enhance pesticide safe usage. This will empower workers to adopt more conscious and safer behaviours while using pesticides.

The hazard and the carcinogenic risks due to the exposure to some potentially toxic elements by the Santiago Island (Cape Verde) population where calculated, considering soil ingestion, inhalation and dermal contact as exposure pathways. The topsoil of Santiago Island is enriched in Co, Cr, Cu, Ni, V, Zn, Mn and Cd to upper crust values. Hazard indices (HI) were calculated for these metals and As exposures, of Santiago Island population and the calculations were performed for children and adults. For children HI are higher than 1 for Co, Cr and Mn. So there is indication of potential non-carcinogenic risk for children, due to the high Co (HI=2.995), Cr (HI=1.329) and Mn (HI=1.126), values in soils. For the other elements and for adults there is no potential non-carcinogenic risk. Cancer risk was calculated for As, Cd, Cr and Ni exposures, for adults and children and the results are always lower than the carcinogenic target risk of 1x10-6, for As, Cd, and Ni. However, cancer risk are higher than the carcinogenic target risk for Cr, for adults. Regarding As, for children the fraction due to Riskingestion represents 51.6%, while Riskinhalation represents 48.0% and Riskdermalcontact represents only 0.4% of total risk. For adults Riskinhalation represents 81.3%, Riskingestion represents 16.6% and Riskdermal contact represents 2.1%. These results reflect the higher daily ingestion dose for children and the higher inhalation rate and higher dermal contact surface for adults. For the other elements and for adults the cancer risk due to Cr, Ni and Cd inhalation is always higher than for children, reflecting the higher inhalation rate for adults.

Anxiety is a multifactorial pathology associated with oxidative stress and changes in different neurotransmitters in the central nervous system (CNS). Sarcomphalus joazeiro (Rhamnaceae) has a predicted effect on the CNS, being considered an alternative for pre-clinical investigations of anxiolytic drugs. The objective of this study is to evaluate the chemical composition, analyze the antioxidant capacity and the anxiolytic effect of the ethanolic extract of S. joazeiro leaves (EEFSJ) in zebrafish. The chemical profile was analyzed by liquid chromatography coupled to mass spectrometry (UPLC-PDA-ESI-QDA). Antioxidant action by DPPH• elimination and ABTS•⁺ capture assays. The 96 h acute toxicity and open field and light and dark tests, for sedative and anxiolytic evaluation, respectively, were applied in vivo, using zebrafish. It was possible to identify 11 compounds from the flavonoid and saponin class. EEFSJ exhibited a mean inhibitory concentration (IC50) of 185.2 ± 2.2 µg/mL and 74.17 ± 1.5 µg/mL against the DPPH• and ABTS•⁺ radicals, respectively. In vivo, EEFSJ demonstrated reduced locomotor activity and an anxiolytic effect similar to diazepam via GABAergic systems, without presenting toxicity within 96 h. These results suggest the safety of using EEFSJ for the development of clinical trials for the production of new anxiolytic drugs.

The efficacy of the product development process is measured by the ability to launch a project with product and production process specifications that could guarantee that the manufacturing can produce it with the least impact. If a problem is detected late, they bring consequences beyond the high cost of the solution, if related to physical ergonomics, which will influence the well-being of operators, productivity, and quality. Virtual Reality (VR) and Digital Human Modeling (DHM) are ones of the enabling technologies of Industry 4.0 and has already been applied on a large scale in industries such as automotive, construction, and aeronautics. However, even though the huge applications, these technologies are not yet applied by these industries for the analysis of physical ergonomics during product development phases. This study aims to characterize the state of the art and technology about the application of Virtual Reality and Digital Human Modeling for the physical ergonomics analysis in the during product development phases in the industry through a systematic review of the literature and patents. In patent documents recovery, we used Derwent Innovation database. The research is based on searching the selected terms in the title, summary, and claims of the documents through a search strategy containing IPC code and keywords. In articles recovery, we searched ScienceDirect, Springer, and IEEExplore databases for scientific publications. The search resulted in 311 patents documents and 16 articles in the scientific database. This study analyzed the patents to map out the technological progress in this area, where we found in the charts and data an increasing number of publications per year and a spread application with a considerable number of new technologies presented in these recent patents. The literature review indicated that Virtual Reality technology complements the Digital Human Modeling during physical ergonomics analysis for manufacturing process already designed. The majority of research on the use of VR and DHM technologies for physical ergonomics analysis focus on the automotive industry and the ergonomic assessment of workstations and current processes. Further research is needed to investigate how Virtual Reality and Digital Human Modeling might assist in the understanding of physical ergonomics in certain tasks throughout the product development process, such as the simulation of worker posture or effort when assembling parts.

Agriculture is the main economic activity of Mozambique and there is lack of information about the quality of agricultural soils. In this paper, five soils from the Manica and Sussudenga districts (Manica province) were sampled in the years 2021/2022 and 2022/2023 (before and after the rainy seasons), were subjected to an agronomical and environmental chemical analysis to assess its quality, either from the fertility and environmental contamination point of view. Standard analytical methodologies from external certified laboratories and local X-ray fluorescence measurements were used. All the studied soils are acidic (pH ranging from 4.5 and 5.4), have no salinity problems (conductivity ranging from 4.2 to 11.8 mS/m), have low amount of soil organic matter (0.90% to 1.81%), and soils from the Sussudenga district have a very low cation exchange capacity (CEC) (average of 3.33 cmolc/kg), while those from Manica district ranges from very low to average CEC (3.59 to 13.11 cmolc/kg). Also, Sussudenga soils have a phosphorous deficiency (values ranging from &lt;20 to 38.5 mg/kg) and there are deficiencies and/or excesses of some macro and micronutrients in all soil samples. Manica soils are contaminated, apparently from geogenic origin, with Cr (280 to 1400 mg/kg), Co (80 mg/kg), Ni (78 to 680 mg/kg) and V (86 mg/kg). Agricultural soils monitoring must be fostered in Mozambique in order to improve food quality and quantity to assure economic and environmental sustainability.

Small island developing states (SIDS) traditionally face a set of challenges like the narrow economic structure, environmental issues, and dependence on a few ranges of economic activities forcing them to open the economy to the exterior. Therefore, their development model, like in Cape Verde depends on migration, remittances, dependence on aid, tourism, and state employment. The current research offers an insight about the nature of Cape Verde’s economy as a SIDS and the degree to which its economy depends on tourism and government services, both of which are supported through aid programs. Understanding Cape Verde’s development model is important to clarify the challenges the country faces, and its development needs to gather a long-term resilience and to understand if it is changing from a MIRAB (Migrations, Remittances, Aid and Bureaucracy) model into another one.

The rationalization and modeling of an electric discharge in a fluid require the knowledge of electron swarm parameters. In this work, transport coefficients of alcohols, including methanol, ethanol, propanol, and butanol are reported. The electron energy distribution function, mean energy, reduced mobility, and diffusion coefficient, are calculated using the two-term expansion of the electron Boltzmann equation in the frame of BOLSIG+ and the Monte Carlo collision code METHES. For such, experimental and theoretical values of the electron-alcohol collision cross-section, covering a range of electron impact energy from 1 eV to 1000 eV, are employed. A temperature of 300K, reduced fields E/N between $1-10^4$ Td (1Td = $10^{-21}~\rm Vm^2$), and Plasma density of $10^{19}~\rm m^{-3}$ were used in simulations. The calculated coefficients are compared with data available from previous studies.

Introduction: Dental segmentation in panoramic radiograph has become very relevant in dentistry, since it allows health professionals to carry out their assessments more clearly and helps them to define the best possible treatment plan for their patients. Objectives: In this work, a comparative study is carried out with four segmentation algorithms (U-Net, DCU-Net, DoubleU-Net and Nano-Net) that are prominent in the medical literature on segmentation and we evaluate their results with the current state of the art of dental segmentation in panoramic radiograph. Methods: These algorithms were tested with a dataset consisting of 1,500 images, considering experiment scenarios with and without augmentation data. Results: DoubleU-Net was the model that presented the best results among the analyzed models, reaching 96.591% accuracy and 92.886% Dice using the dataset with data augmentation. Another model that stood out was Nano-Net using the dataset without data augmentation; this model achieved results close to that of the literature with only 235 thousand trainable parameters, while the literature model (TSASNet) contains 78 million. Conclusions: The results obtained in this work are satisfactory and present paths for a better and more effective dental segmentation process.

Keywords: Wood residuals; Forest biomass; Wood composite; Wood floor; Life Cycle Assessment.

Nowadays, we can observe a worldwide trend towards the development of synthetic biomaterials. Numerous researches have been carried out in order to better understand the cellular behavior involved in the inflammation and bone healing processes related to living tissues. The aim of this study is to evaluate the tissue behavior of two different types of biomaterials: synthetic nano-hydroxyapatite / beta-tricalcium phosphate and hydroxyapatite in non-critical bone defects in rat calvaria. Twenty four rats underwent surgery in which two 3 mm defects in each cavity were performed. The rats were divided into 2 groups: Group 1, using xenogen hydroxyapatite (Bio oss ™); Group 2, using synthetic nano-hydroxyapatite / beta-tricalcium phosphate (Blue Bone ™). Sixty days after the surgery, the calvaria bone defect was filled with biomaterial, the animals were sacrificed and marked with Masson's trichrome and PAS staining, and immunohistochemistry (TNF-α, MMP-9) and electron microscopy analyzes were performed. Histomorphometric analysis indicates a greater presence in the protein matrix in Group 2, in addition to higher levels of TNF-α, MMP-9. The ultrastructural analysis shows an association of material with fibroblasts in the tissue regeneration stage. Paired statistical data indicates that Blue Bone ™ can improve bone formation / remodeling when compared to biomaterials of xenogenous origin.

Over the last two decades, a considerable amount of equipment has been acquired (spreaders, seeders, sprayers, among others) to respond to the challenges of Precision Agriculture (PA) concept. Most of this equipment has been purchased at a high cost. However, many of them, despite still being functional and equipped with sensors, actuators, and electronic processing units capable of adjusting to variations in speed, have become obsolete in terms of communication, and incompatible with new monitoring and control systems based on the “Isobus” protocol. This work aims to present a solution for updating the control system (“Ferticontrol”) of a “Vicon RS-EDW” spreader with variable rate application (VRA), making it compatible with the “InCommand” system from “Ag Leader”. The solution includes low-cost “Arduino” and “Raspberry Pi” microcontrollers and open-source software. The development shows that it is possible to implement a solution that is accessible to farmers in general. It also provides a niche business opportunity for young researchers to set up small technology-based enterprises associated with universities and research centers. These partnerships guarantee permanent innovation and represent a decisive step towards modern, technological, competitive, and sustainable agriculture.

We hypothesize the possible route of introduction of the cosmopolitan DENV-2 genotype into Brazil through the border with Peru. The five sequences presented in this study create a geographical link with the three others sequences of this genotype already recorded on the continent.

Mozambique is one of the poorest countries of the world. However, it has natural resources and if they are managed under sustainable development principles, Mozambique can overcome the current problems. In the present socio-economic status, soil is one of its most important resources and must be protected from pollution and from degradation. This review focuses on the identification and discussion of the main soil quality stressors, namely: soil fertility; deforestation and its sources: agriculture, timber harvesting, charcoal production and uncontrolled fires; mining activities, manly gold artisanal explorations and industrial open-pit coal mining. The sustainable use of the natural resources is mandatory to allow future generations to continue profiting for this nature-based wealth.

In the bioprinting process, controlling the motion of bioprinters involves a computer-aided design (CAD) model, converting that model into g-code, and transmitting the motion commands to the bioprinters. The g-code file contains information about the motion of the axes and can be generated using various software. However, a challenge arises when using g-code between different bioprinters. Therefore, the objective of this study was to propose a reliable method for analyzing the differences in 3D bioprinting devices by parameterizing a crucial variable for extrusion bioprinting: the E-value. Four bioprinters from three different manufacturers were used to investigate the extrusion behavior when subjected to the same E-value.

The understanding of the issues that affect the training of information professionals requires reflection on the impact of Digital Information and Communication Technologies in the current working world, characterized by the rupture of the notion of space / time and the crossing of jurisdictions and professional boundaries, where dilemmas pedagogies to qualify these professionals considering future perspectives. It was based on the assumption that "studies of graduates" are subsidies for the evaluation of the educational system in face of the demands of in the labor market, helping in the progression of the researches in information, education and work. The objective was to map the results obtained in the studies of graduates promoted in the postgraduate and undergraduate courses in Information Science and Librarianship to verify if the demands pointed out in the surveys with graduates are in line with the studies of Education and Training, the orientations of the National Curricular Guidelines (DCN) and the regulatory actions of the Federal Library Board (CFB). The sample consisted of 45 studies published in CI journals on their associated areas, in the Brazilian context, from 2000 to 2018, of which only 10 articles related to the Library Sciences were considered for the qualitative analysis of this initial phase. to enable a comparative analysis between the proposals of the DCN, the critical analysis of the academic area of ​​CI in the studies on education and professional formation (pedagogical projects and curricula) and the orientations of the class councils. The results were categorized according to the reflection of the graduates about their education, the experience of education, vision about professional practice and the labor market; and followed the structure of the DCN axes: profile; skills and abilities; curricular contents, internships and complementary activities; course structure and institutional evaluation. Prevalence of studies on the identification of graduates (gender, age group, employment and work place) was predominant; few focused on salary aspects; and there were no questions about cultural habits or perceptions about changes in the world of work. Results on issues related to job placement and employability have indicated the need to expand the range of possibilities that go beyond traditional information units and the importance of internships for understanding new virtual work spaces. The dimension of the academic formation was evaluated questioning the compatibility of the disciplines with the demands of the labor market and the satisfaction of the graduates with the course and profession, being indicated as more important contents for the professional life the more technical disciplines and that characterize the course of Librarianship, complemented by those related to management; and with less relevance to the language subjects; marketing; statistic; information and information technology, as opposed to the flexibility proposed by the DCN, adopted after 2002. It is concluded that the process has been guided by isolated initiatives, and that the collective debate involving university and the professional world lacks actions for the construction of dialogue, which may result in the definition of pedagogical and curricular projects suited to the demands of graduates in their regions of origin and demands of the world of work.

The objective of this study was to evaluate the genetic effects involved on pepper fruit post-harvest water loss, and correlated to morphology traits. Fruits of eight landraces of C. baccatum, and their 28 hybrids, were evaluated in a randomized complete block design. Analysis of variance, diallel analysis, phenotypic and genotypic correlation were performed to compare fruit water loss (WL), width (FW), length (FL), total soluble solids (TSS), dry matter content (DMC), pericarp thickness (PT), cuticle thickness (CT), and exocarp thickness (ET). WL varied from 14 to 68% during storage. Non-additive effects played more important role than additive, including CT, EP and TSS. Effective strategies to reduce WL should be achieved by repeated backcrosses followed by selection in segregating populations (SP). Therefore, the selection of hybrids should follow high specific combining ability traits. Selecting fruits with higher CT and lower TSS values are indirectly selecting fruits with lower WL. Varieties with low WL can be breed using landraces 04 and 58 as parents or by the hybrid 04 x 44 in open lines in SP. Brazilian C. baccatum landraces are a source of genetic variability, and the SP emerging from crossing lines with reduced WL should develop new ways for conventional breeding.

Abstract. Background: COVID-19's first victim was announced by Chinese health authorities on the 11th of January 2020. On January 13, the first official case was reported outside China, in Thailand. On January 25, the same occurred in São Paulo and on March 8, the first case was recorded in Minas Gerais. From that point onwards until the 3rd of October 2020, a total of 370,911 cases and 9,204 deaths were recorded in the state. This study aims to investigate spatiotemporal patterns of COVID-19 with incidence from March 22 to October 3 of 2020. Methods: The database itself was obtained from Health Division of Minas Gerais state. The vulnerability index was calculated using a principal component analysis. Moran's I autocorrelation was tested, z-score and P-value < 0.05. Results: From March 22 to October 3 of 2020 the incidence level varied from 45.680/100,000 to 312.130/100,000. The most influential variables were: illiteracy, gross domestic product and breath apparel per municipality. The clusters were concentrated in the metropolitan area of Belo Horizonte, Zona da Mata and Triangulo Mineiro. Conclusion: The spatial distribution of COVID-19 from week 13 until week 40 showed that different levels of endemicity and mesoregional vulnerabilities were represented in these maps.

The cell surface targeting of neo-synthesized G protein coupled receptors (GPCRs) involves recruitment of receptor into COPII vesicles budding at Endoplasmic Reticulum Exit sites (ERES). This process is regulated for some GPCRs by escort proteins, which facilitate their export, or by gatekeepers that retain the receptors in the ER. PRAF2, an ER gatekeeper of the heterodimeric GABAB receptor protomer GB1, which interacts with a tandem di-leucine / RXR retention motif in the carboxyterminal tail of GB1, also inhibits the plasma membrane export of the chemokine receptor CCR5. PRAF2 / CCR5 interactions involve the transmembrane domains of both proteins. The di-leucine / RXR motif contained in the third intracellular loop of CCR5 does not affect PRAF2-mediated retention but impairs instead the interaction between CCR5 and its private escort protein CD4. PRAF2 and CD4 thus display opposite roles on the cell surface export of CCR5, likely at the level of its recruitment into COPII vesicles, PRAF2 inhibiting and CD4 promoting this process.

Soarinho Alkaline Intrusion, southeastern Brazil, makes part of the Serra do Mar Igneous Province, and it is composed of alkali feldspar syenite, alkali feldspar trachyte, quartz syenite, and mon-zonite. Geochemical and geochronological analysis of the Soarinho were compared with data from its neighbors Rio Bonito and Tanguá. Zircon U-Pb Laser Ablation ages show that Soarinho is youngest than Rio Bonito and Tanguá. Ages obtained from the monzonite (60 ± 2 Ma), alkali feldspar syenite (58 ± 2 Ma), and quartz syenite (58 ± 2 Ma) are indistinguishable within error. Petrographic and geochemical data show a clear contrast among Soarinho, Rio Bonito, Tanguá, and Lu-Hf isotopic data for the three alkaline intrusions (all negative), point to some similarities in their sources. The data suggest an enriched mantle source for Soarinho; however, an older con-tinental crust contribution cannot be discarded.

Infectious bronchitis virus (IBV) is a highly variable RNA virus that affects chickens worldwide. Due to its inherited tendency to suffer point mutations and recombination events during viral replication, emergent IBV strains have been linked to nephropathogenic and reproductive disease that are more severe than the typical respiratory disease, leading, in some cases, to mortality, severe production losses, and/or unsuccessful vaccination. QX and DMV/1639 strains are examples of the above-mentioned IBV evolutionary pathway and clinical outcome. In this study, our purpose was to systematically compare whole genomes of QX and DMV strains looking at each IBV gene individually. Phylogenetic analyses and amino acid site searches were performed in datasets obtained from GenBank accounting for all IBV genes and using our own relevant sequences as a basis. The QX dataset studied is more genetically diverse than the DMV dataset, partially due to the greater epidemiological diversity within the five QX strains used as a basis compared to the four DMV strains from our study. Historically, QX strains have emerged and spread earlier than DMV strains in Europe and Asia. Consequently, there are more QX sequences deposited in GenBank than DMV strains, assisting in the identification of a larger pool of QX strains. It is likely that a similar evolutionary pattern will be observed among DMV strains as they develop and spread in North America.

Stainless steel 316L (SS316L) and titanium-alloy Ti6AL4V exhibit properties for bio-medical applications; however their tribocorrosion associated with metallosis is still a major concern. Therefore smoothing DLC coatings aiming for the improvement of tri-bocorrosion and cell viability of stainless steel 316L (SS316L) and titanium-alloy Ti6AL4V with and without DLC coatings were compared. The motivation was due to many papers detailing metallosis caused by metal debris in the body's soft tissues. Nowadays, metallosis is responsible for almost of prosthesis replacement surgeries. DLC coatings were produced using the Plasma Enhanced Chemical Vapor Deposition (PECVD) technique; acetylene gas was used as a carbon precursor, and silane gas produced an amorphous silicon interface between the DLC coating and metal substrate. A combined setup of techniques thoroughly evaluated the coatings considering chemical composition, coatings adhesion at a metallic substrate, scratch resistance, and tribocorrosion susceptibility. The cytotoxicity and genotoxicity of metallic samples with and without DLC coatings were analyzed, considering cell viability and its correlation with coatings’ chemical composition

Biomass-derived carbon dots (CDs) are gaining much interest in recent times as it is a sustainable option with abundant availability, low cost, and tunable luminescence. Herein, we report a simple green synthesis method to produce highly fluorescent CDs from Eucalyptus globulus leaves using the one-pot hydrothermal approach. The fabricated CDs exhibit strong blue fluorescence with an excitation and emission maxima of 340 nm and 442 nm respectively. The highest quantum yield (QY) obtained was 60.7%. Due to its promising optical properties and biocompatibility, CDs can be a potential candidate for biosensing applications. Moreover, we employed a life cycle assessment (LCA) cradle-to-gate approach to study the environmental impacts of the synthesis strategy used for the fabrication of CDs. The results point out that citric acid is a main hotspot in CDs synthesis, regarding environmental impacts in most categories. This justifies the introduction of biomass, which reduces the amount of citric acid, thus leading to a more sustainable synthesis strategy for fabricating CDs.

BDD is a hoof disease characterized by inflammation of the second and fifth accessory digits and the skin in this region. This pathology is scarcely described in the literature; however, it has recently been observed in beef cattle in the Amazon Biome region. The objective of this study was to identify the epidemiological factors associated with BDD onset in cattle in the analyzed biome. Samples were collected from eight farms with extensive breeding systems located in Xinguara, Rondon do Pará, Curionópolis, and Ipixuna do Pará, in the state of Pará, Brazil. A total of 706 Nellore and Nellore crossbred with taurine animals of both sexes were evaluated, with males aged between 2–4 years and a mean weight of 650 kg and females aged between 2–11 years and a mean weight of 400 kg. Distal extremities were inspected during cattle management, and in case of accessory digit lesions, a specific examination was carried out after proper restraint. Animals were diagnosed with BDD in all farms analyzed. Of the 706 cattle inspected, 49 (6.94%) showed BDD, of which 19 (38.77%) were Nellore and 30 (61.22%) were crossbred. This was the first study to determine BDD’s occurrence in extensive farming systems in the Amazon region, also showing that pastures with large amounts of stumps and stones, the physical structure of pens, and trauma and injury incidence during animal management are the most important predisposing factors for the onset of BDD.

The fourth industrial revolution (4IR) can offer significant benefits to the construction sector, improving productivity, efficiency, collaborative efforts, and product quality while promoting safety and sustainability. However, research on the application of 4IR technologies in construction is scarce in developing countries. It is crucial to understand the ability of construction companies to adopt new technologies and identify factors influencing the success of technology implementation. In this study, a questionnaire-based survey was conducted with construction professionals to evaluate the level of technological development of the construction market in an emerging economy, assess the potential for innovation implementation, and identify factors that might influence technological development. Although respondents recognized the importance of digital transformation, the results showed that most innovations are in the early stages of implementation in the construction sector, particularly technologies that are essential for the consolidation of the 4IR. Understanding the factors underlying this reality can support the development of strategies to foster innovation and harness the advantages of the 4IR in the construction industry in developing countries.

The advancement of mechanization in forestry has increased productivity in the forestry sector, bringing positive and negative impacts that require a deeper understanding for sustainable forest management. This study aimed to apply a simplified instrument for assessing damage and environmental impacts in forest harvesting of commercial eucalyptus plantations, using a combination of methodologies. The methodology used combined interaction networks and impact assessment matrices, carrying out field surveys, transposing them to interaction networks and weighting them through assessment matrices, resulting in environmental indices (ES) for prioritizing actions. The study was conducted on a commercial eucalyptus plantation in the municipality of São Pedro, São Paulo, Brazil. The mechanized harvesting of the area consists of the structure of a module with a mobile unit consisting of a harvester and forwarder. The results indicated that wood transport presented the highest ES, both positive and negative. The most significant negative impacts (ES) were the depletion of water resources and erosion, while the positive impacts included regional development and job creation. The most notable changes, positive and negative, were observed in the physical and anthropic environment, with a lesser impact on the biotic environment.

The present work analyzes the behavior in terms of corrosion resistance of three reinforced concrete formulations over a period of 1 year. The samples were accompanied by creating a monitoring methodology using the Electrochemical Impedance Spectroscopy (EIE) technique, working only with the real component over time. Three mixtures were used, one conventional without pozzolanic addition (REF) and two others with pozzolanic additions, rice husk ash (RCA) and metakaolin (MK). Prototypes were created and exposed to the action of a 165g/l NaCl sodium chloride solution. The characterization of the materials was carried out by determining the chloride diffusion profile (ASTM 1556), analyzing images using tomography and with the support of analytical techniques such as x-ray fluorescence and x-ray diffraction. The monitoring methodology using the EIE demonstrated the positive effect of the insertion of pozzolans, rice husk ash (RCA) and metakaolin (MK), by delaying the process of chloride diffusion in the concrete, resulting in greater resistance to corrosion. The EIE also showed that the active mineral addition in concrete, of aluminum-silicic composition (MK), had a predominant protective effect on the steel//concrete interface against the attack of chloride ions.

Micro-nano-plastics (MNPs) are an important constituent of atmospheric aerosol. However, there is still no standard procedure for their sampling and size fractionation, which is an obstacle to the aggregation and critical analysis of results obtained by different research groups. This review focuses on the sampling and fractionation methodologies used for MNPs. Moreover, a straightforward optimized methodology for the sampling and fractionation is proposed.

In this work, the influence of catalyst reuse and reaction time on the yield and quality of organic liquid products (OLP) obtained in a cracking pilot plant at 450 °C and 1.0 atm has been systematically investigated. The distillation of OLP produced 04 (four) distilled fractions. The quality of the biofuels was certified by physical-chemical analysis, as well as FT-IR and GC-MS analysis. The experimental results showed the feasibility of applying the spent sodium carbonate twice in catalytic cracking of vegetable oils. The physical-chemical properties (density, viscosity, acid value, saponification value, and flash point) of OLP decrease as the reaction time increases. The distillation of OLP yields 62.35% (wt.), producing green-like gasoline, kerosene, and diesel fractions rich in hydrocarbons. Therefore, biofuels-like fractions produced by distillation of OLP have a great potential for replacing partially petroleum derived fuels.

We report the characterization of phenotype, genotype, and functional parameters such as CD4+ T cell, viral load, HIV-1 genotype, class I HLA genotype, CCR5 polymorphism, and IFN-γ producing cells from a cohort of 37 people living with HIV-1 (PLWH) (viremic controllers, were compared those on antiretroviral therapy (ART) from two sites in São Paulo, Brazil. Half the PLWH (51.4%) were classified as viremic controllers (Controller group - CG), compared to 48.6% were on ART. As expected, controllers showed baseline higher levels of CD4+ T cells and nadir compared to those under ART, confirming the ability of HIV-1 controllers to preserve these cells even in the absence of ART. IFN-γ-producing cells showed greater magnitude to the HIV peptides Gag in controllers (p<0.03). Our results demonstrated that HLA protective alleles are associated with strong production of IFN-γ to Gag, Nef and RT epitopes in people living with HIV controllers.

The whitefly leads extensive damage to plants through direct feeding, honeydew secretion, plant physiological disorders, and vectoring plant viruses. This study aimed to evaluate the physiological characteristics of susceptible and resistant soybean cultivars to B. tabaci. The experiments were conducted in a greenhouse. Eleven soybean cultivars were selected and infested with 100 adults of B. tabaci at the V3 stage. The evaluation of photosynthetic parameters, such as photosynthetic rate, leaf transpiration, stomatal conductance, and internal CO2 concentration, revealed that B. tabaci infestation influenced gas exchange in soybean plants. The photosynthetic rate was higher in cultivars AS3810 and M8349 during the V6 stage. Infestations led to alterations in photosynthetic parameters, suggesting increased energy demand to maintain photosynthetic activity. However, the response to infestation varied among different cultivars, indicating varying levels of resistance and tolerance to the whitefly's damage. Additionally, the impact of infestation was more significant during the vegetative phenological. In conclusion, B. tabaci infestation affects soybean plants' physiology, leading to changes in gas exchange parameters and water use efficiency. The response to infestation varied among soybean cultivars, suggesting potential differences in resistance to the pest. The study highlights the importance of evaluating the physiological impact of whitefly infestations on soybean.

Biocomposite membranes based on polylactic acid (PLA) and cellulose nanocrystals (CNCs) were developed using a scientific approach. Dicumyl peroxide (DCP) was used as a polymerization ini-tiator, while tin octoate (Sn(Oct)2) and triphenylphosphane (P(C6H5)3) were used as catalysts. A torque rheometer was used to mix the components of the biocomposite, and thin films was prepared by solvent casting. Fourier transform infrared (FTIR) spectroscopy confirmed the coupling between the PLA and CNCs. Field emission scanning electron microscopy (FESEM) showed that the CNCs were well-dispersed in the PLA matrix with an unimodal particle size distribution and a maximum particle size of around 200 nm. Thermogravimetric analysis (TGA) and differential scanning calo-rimetry (DSC) analysis demonstrated good thermal stability and improved biodegradability of the biocomposite membrane compared to pure PLA. Mechanical characterization showed a Young's modulus of 1.65 GPa, which is comparable to that of other composite materials, and a maximum tensile strength of 20.31 MPa, which is higher than that of pure PLA. These results suggest that the developed biocomposite membrane has potential applications in water filtration, food packaging, and biomedical devices.

A Robust Optimization framework with original concepts and fundamentals also admitting a fusion of ideals from relative regret models and static robust optimization, containing conservatism concepts is disclosed. The algorithm uses a fine-tune strategy to tune the model so the robustness and a target ideality can be mutually achieved with a specified risk. The framework comprises original concepts, a mathematical approach and an algorithm. The statistical treatment of the data with the original concepts from the framework make it able to make short, middle or long-term decision-making setting. The framework has high tractability since the algorithm forces the creation of a setting that makes a robust optimization with the specified risk. The framework can be applied in linear and nonlinear mathematical models since that the objective function is monotonic in the domain of the active convex region. Several examples are solved to best understand the framework and all results demonstrated high tractability and performance. There is a wide range of applications. Along all the text, there is a profound discussion about its philosophy, objective, original concepts, fields of application, statistical and probabilistic fundamentals.

The consumption of fiber in the human diet is a global recommendation to ensure a healthy diet. Quinoa (Chenopodium quinoa Willd.), a gluten-free grain, and chia (Salvia hispanica), a seed, contain high fiber content and both have the potential to be explored in the development of nutraceutical and pharmaceutical formulations. An interesting characteristic of chia is its ability to form viscous mucilage when in contact with water, bringing the prospect of a binder in solid formulations. To produce tablets with a high fiber content, it is more appropriate to transform the material into granules using a wet process, with the help of a tool that exploits this possibility. Therefore, the objective of the present work was to study, using the Mixer Torque Rheometer, the feasibility of using chia as a binder to produce granulated quinoa and the development of chewable tablets. To this end, an experimental design was carried out to evaluate the impact of variables on the rheometer results for subsequent granulation. It was possible to obtain the granulation point for the formulations and after producing the granules, physical tests were carried out in which we noticed improvements in the flow. In the end, a formulation was selected for the development of chewable tablets containing quinoa and chia fibers.

Since it was proposed, the Robot Operating System (ROS) has fostered solutions for various problems in robotics in the form of ROS packages. One of these problems is Simultaneous Localization and Mapping (SLAM), a problem solved by computing the robot pose and a map of its environment of operation at the same time. The increasingly availability of robot kits ready to be programmed and also of RGB-D sensors often pose the question of which SLAM package should be used given the application requirements. When the SLAM subsystem must deliver estimates for robot navigation, as is the case of applications involving autonomous navigation, this question is even more relevant. This work introduces an experimental analysis of GMapping and RTAB-Map, two ROS compatible SLAM packages, regarding their SLAM accuracy, quality of produced maps and use of produced maps in navigation tasks. Our analysis aims ground robots equipped with RGB-D sensors for indoor environments and is supported by experiments conducted on datasets from simulation, benchmarks and from our own robot.

In this study, an extensive data set was based on existing literature records in order to enable the suitability of several predictive models, from Multiple Linear Regression (MLR) to Neural Networks (NN). The main objective was to, through regression analyses, generate model computations to correlate tensile properties (UTS- Ultimate Tensile Strength, YTS – Yield Tensile Strength and EF – Elongation-to-Fracture) to a given alloy composition and microstructural spacing. This investigation led to positive results, as the highest accuracies of the trained modules (in 80% of the database) were found to be above ~82% (UTS and EF) and a maximum of ~98% (YTS), when analyzing the results to a test data set. Later, these models were used to define trends for possible next solder alloy commercial compositions. Overall, using the standard model’s setup, the Random Forest and Decision Tree models showed the highest accuracy results, with 0.958 for YTS as opposed to 0.907 for MLR. Moreover, Multilayer Perceptron (MLP)-optimized models yielded the best results for each variable, with the highest increases in accuracy associated with the YTS and EF. The present contribution might imply an important milestone towards alloy design research based on data science guidelines to unlock the full potential of former experiments and their extensive set of results.

Musculoskeletal disorders are increasingly prevalent worldwide, causing significant socioeconomic burdens and diminished quality of life. Notably, patellar chondropathy (PC) is among the most widespread conditions affecting the joint structures, resulting in profound pain and disability. Hyaluronic acid (HA) and platelet-rich plasma (PRP) have emerged as reliable, effective, and minimally invasive alternatives. Continuous research spanning from laboratory settings to clinical applications demonstrate the numerous advantages of both products. These encompass lubrication, anti-inflammation, and stimulation of cellular behaviors linked to proliferation, differentiation, migration, and the release of essential growth factors. Cumulatively, these benefits support the rejuvenation of bone and cartilaginous tissues, which are otherwise compromised due to the prevailing degenerative and inflammatory responses characteristic of tissue damage. While existing literature delves into the physical, mechanical, and biological facets of these products, as well as their commercial variants and distinct clinical uses, there is limited discussion on their interconnected roles. We explore basic science concepts, product variations, and clinical strategies. This comprehensive examination provides physicians with an alternative insight into the pathophysiology of PC as well as biological mechanisms stimulated by both HA and PRP that contribute to tissue restoration.

Dipteryx spp. is an important species in reforestation in the Amazon. The objective of this study is to characterize and compare the relationships between dendrometric variables in Dipteryx spp. stands in the Western Amazon by fitting linear regression equations for total height and crown diameter. Six forest stands were evaluated in three municipalities. Dendrometric variables collected included diameter at 1.3 m height (dbh), total height (ht) and crown diameter (dc). Simple and multiple linear regression equations were fitted to characterize the relationships between ht and dc. The total aboveground biomass of Dipteryx spp. trees and the carbon stock of the stands were estimated. The general equations showed higher R² values, exceeding 0.7. The general equations for estimating ht and dc were significant for all coefficients. The trees averaged 22 t/ha of aboveground biomass in the stands. There was a variation in carbon sequestration potential among stands, ranging from 5.12 to 88.91 t CO2.ha-1. Single-input equations using dbh as an independent variable are recommended for estimating dc and ht for individual Dipteryx spp. stands. Stands in the Western Amazon play a significant role in carbon sequestration and accumulation. Trees can sequester an average of 4.8 tons of CO2 per year.

The objective was to evaluate the metabolic and performance differences in primiparous Nellore cows, which became pregnant at 14 or 24 months. Thirty-eight primiparous Nellore cows, which were bred for first pregnancy at 14 or 24-mo of age, with 202 ± 5 days of gestation were divided into two treatments according to breeding age: 14 or 24 months in the breeding season. Cows were evaluated for weight, body condition score (BCS), carcass characteristics, milk yield and blood parameters: glucose, total proteins, albumin, urea, beta-hydroxybutyrate (β-OHB), non-esterified fatty acids (NEFA), progesterone, and for insulin-like growth factor-1 (IGF-1). There was an in-teraction (p < 0.01) between age at first pregnancy and time for body weight, where 24-mo cows were heavier, but both categories loss weight at parturition which concomitant decrease in the BCS (p < 0,01). However, BW of 24-mo remained stable (p > 0.05) after parturition. Milk yield was higher in 24-mo cows (p < 0.01). Interaction between sampling time and breading age was observed for Longissimus muscle area (p < 0.01) and tended for Longissimus muscle depth, where 24-mo cows had greater values in prepartum. Calves born from the cows bred at 24-mo, where was higher (p < 0.01) from 160 days of age until weaning at 240 days. Glucose, urea, total proteins, albumin and NEFA were affected (p < 0.01) by the parturition data-point. There was an interaction (p < 0.01) between breading age and sampling period for β-OHB. Progesterone concentration was greater in 24-mo cows (p < 0.01). It was concluded that primiparous cows that became pregnant at 14 or 24 months of age have different nutritional and metabolic responses.

This study analyzes the evolution of Corporate and Social Responsibility (CSR) and Environmental, Social, and Governance (ESG) concepts in social media, specifically on Twitter, from 2007 to 2022. The research aims to understand how society perceives organizational practices related to these aspects. Twitter provides an authentic environment for capturing user perceptions. The methodology involved collecting Tweets using Python and analyzing them in English, Spanish, and Portuguese with RStudio. The study's main findings highlight significant contributions and concerns within the CSR and ESG debates. While CSR's popularity showed a downward trend from 2010 to 2019, ESG grew exponentially during the pandemic and post-pandemic periods. Word analysis revealed the impact of specific hashtags: RSC in Spanish, #CSR in English, and #ESG in Portuguese. Society's increasing concerns concern environmental issues for combating global warming and ensuring a better quality of life, alongside financial transparency and ethical practices in companies. However, the social aspect of ESG, represented by the letter "S," didn't garner the same attention as environmental and governance matters. This discrepancy might stem from a lack of awareness or lower priority in public discussions on social media, overshadowing the social dimension in the ESG framework.

Improving antigen presentation is crucial for the success of immunization strategies. Yeasts are classically used as biofactories to produce recombinant proteins and are efficient vehicles for the delivery of vaccine antigens, besides present adjuvants properties. Despite the absence of epidemic outbreaks, several vaccine approaches continue to be developed for Zika virus infection. These prophylactic strategies are fundamental given the severity of clinical manifestations, mainly due to viral neurotropism. The present study aimed to evaluate in vivo the immune response induced by P. pastoris recombinant strains displaying epitopes of the Envelope (ENV) and NS1 ZIKV proteins. Intramuscular immunization with heat-attenuated yeast enhanced the secretion of IL-6, TNF-α, and IFN-γ, besides activation of CD4+ and CD8+ T cells, in BALB/c mice. P. pastoris displaying ENV epitopes induced a more robust immune response, increasing immunoglobulin production, especially IgG isotypes. Both proposed vaccines showed the potential to induce immune responses without adverse effects, confirming the safety of administering P. pastoris as a vaccine vehicle. Here we demonstrated, for the first time, the evaluation of a vaccine against ZIKV based on a multiepitope construct, using yeast as a vehicle, reinforcing the applicability of P. pastoris as a whole yeast cell vaccine.

Thrombosis is characterized by the pathological formation of fibrin clots within a blood vessel, leading to the obstruction of blood flow. Fibrinolytic enzymes from microorganisms have exhibited promising effects to dissolve clots in a more efficient and safe way. Then, the aim of this study was to evaluate the biomass and fibrinolytic enzyme production of Tetradesmus obliquus under autotrophic and mixotrophic conditions using different concentration of corn steep liquor (CSL). Different extraction and precipitating methods were tested, and the enzyme was purified by ion exchange chromatography. More advantageous culture condition was mixotrophic using 0.25% CSL, showing the highest values of biomass productivity (Px = 169.3 ± 44.36 mg∙L-1day-1) and specific growth rate (µmax = 0.17 ± 0.00 day-1), and significant fibrinolytic production (391.34 ± 40.03 U∙mg-1). Moreover, fibrinolytic activity was higher when extracted by homogenization and precipitated using acetone, which exhibited clear zone of fibrin degradation in the fibrinolytic plate assay. Additionally, the purified enzyme showed specific activity of 1176.90 ± 140.37 U∙mg-1 and molecular weight around 97 kDa. Finally, the enzyme has higher enzymatic activity than various fibrinolytic enzymes, and the obtained enzyme has potential to be developed as a therapeutic agent in thrombosis treatment. Additional studies are need to investigate the biochemical properties and biological profile of this enzyme.

The structure of wound dressing materials presents one of the most relevant characteristics for effective skin tissue repair. Electrospinning is a common technique used to produce polymeric fibres that can mimic fibrillar disposition of skin extracellular matrix, favouring cell migration, and thus regeneration of the damaged tissue. Moreover, beads, also known as by-products of electrospinning, have potential as reservoirs for sustained drug release. Processing parameters, such as molecular weight and viscosity of the polymer solution, can affect the desirable morphologies of electrospun films. Thereby, this work had the purpose of producing and characterized electrospun polycaprolactone (PCL) mats loaded with propolis, a popular extract in traditional medicine with potential for skin repair aid. Films with different morphologies were obtained depending on the storage period of the solution prior to the lectrospinning, probably due to the PCL hydrolysis. FTIR analyses of the extract confirmed propolis composition. GPC and viscosity analyses demonstrated that the decrease in molar mass over the storage period was responsible for nanostructure diversity. Propolis acts as a lubricant agent, affecting the spun solutions' viscosity and the thermal properties and hydrophilicity of the films. All films are within the value range of the water vapour transpiration rate of the commercial products. The presence of beads did not affect the propolis release pattern. However, "in vitro" wound healing assay showed that propolis-loaded films composed by beaded fibres increased the cell migration process. Thus, it can be inferred that these films presented the potential for wound dressing application.

Magnetic resonance elastography (MRE) has revealed sexual dimorphism in brain stiffness in healthy individuals and multiple sclerosis (MS) patients. In the animal model of MS, experimental autoimmune encephalomyelitis (EAE), we showed previously that inflammation-induced brain softening was associated with alterations of the extracellular matrix (ECM). However, it remained unclear whether the brain ECM presents sex-specific properties that can be visualized by MRE. Therefore, we aimed here at quantifying sexual dimorphism in brain viscoelasticity in association with ECM changes in healthy and inflamed brains. Multifrequency MRE was applied to the midbrain of healthy and EAE mice of both sexes to quantitatively map regional stiffness. To define differences in brain ECM composition, gene expression of the key basement membrane components laminin (Lama4, Lama5), collagen (Col4a1, Col1a1) and fibronectin (Fn1) was investigated by RT-qPCR. We showed that the healthy male cortex expressed less Lama4, Lama5, Col4a1 but more Fn1 (all p < 0.05) than the healthy female cortex, which was associated with 9% softer properties (p = 0.044) in that region. At peak EAE, cortical softening was similar in both sexes compared to healthy tissue, with an 8% difference remaining between males and females during EAE (p < 0.001). Cortical Lama4, Lama5 and Col4a1 expression increased 2 to 3-fold in EAE in both sexes while Fn1 decreased only in males (all p < 0.05). No significant sex differences in stiffness were detected in other brain regions. In conclusion, sexual dimorphism in the ECM composition of cortical tissue in the mouse brain is reflected by in vivo stiffness measured with MRE and should be considered in future studies by sex-specific reference values.

Immersive Unit Visualization is an emergent form of visualization that arose from Immersive Analytics where, unlike traditional visualizations, each data point is represented by an individual visual mark in an immersive virtual environment. This practice has focused almost exclusively on virtual reality, excluding augmented reality (AR). This article develops and tests a prototype of an immersive Unit Visualization (Floating Companies II) with two AR devices: head-mounted display (HMD) and hand-held display (HHD). Results from the testing sessions with 20 users were analyzed through qualitative research analysis and thematic coding indicating that, while the HHD enabled a first contact with AR visualization on a familiar device, HMD improved the perception of hybrid space by supporting greater stability of virtual content; wider field of view; improved spatial perception; increased sense of immersion; and more realistic simulation, which impacted on information reading and sense-making. The materialization of abstract quantitative values into concrete reality through its simulation in the real environment and the ludic dimension stand out as important opportunities of this type of visualization. This paper investigates the aspects distinguishing two experiences regarding data visualization in hybrid space; and characterizes ways of seeing information with AR, identifying opportunities to advance information design research.

This paper investigates an Energy Neutral System using Micro Energy Harvesters for a Health Monitoring Wireless Sensor Node (HM-WSN). We have implemented HM-WSN consisting of a solar cell energy harvester, a Power Management Unit (PMU) and the ucontroller, sensor and transmitter acting as load. A battery extender and a switching logic circuit for sensor node is designed to evaluate the efficiency of the proposed system. The HM-WSN senses body temperature with improvement in lifetime of coincell. The HM-WSN is built using commercially available off the shelf (COTS components and consumes 47.2uW for data being updated every 134s. It can be operated for approximately 1.32 years only on a coincell. Furthermore, the HM-WSN can be operated with solar energy at 200lux, achieving 96.36 years with a combination of a coincell with an additional capacitor of 2.2mF. It attains 4.07 years to 16.87 years lifetime with the load capacitor varying from 1mF to 1.5mF at 350lux lighting condition. With the same ambient condition and a 2mF load capacitor, HM-WSN attains complete energy autonomy.

Over the last decades, the increased incidence of metabolic disorders such as type 2 diabetes and obesity has motivated researchers to investigate new enzyme inhibitors. In this study, the inhibitory effects of synthetic amino acid derivatives (PPC80, PPC82, PPC84, PPC89, and PPC101) on the activity of digestive enzymes was assessed by in vitro assays. The inhibitory effect was determined by the inhibition percentage and the 50% inhibitory concentration (IC50), and the mechanism of action was investigated by Lineweaver–Burk plots. PPC80, PPC82, and PPC84 inhibited pancreatic lipase (IC50 of 1.67–10.23 x 10-1 mmol/L). The activity of pancreatic α-amylase was suppressed by PPC80, PPC82, PPC84, PPC89, and PPC101 (IC50 of 1.62–5.19 x 10-1 mmol/L). Finally, PPC84, PPC89, and PPC101 also showed a potent inhibitory effect on α-glucosidase (IC50 of 0.51–3.53 x 10-1 mmol/L). PPC80 and PPC82 followed a non-competitive inhibition mechanism against pancreatic lipase, while PPC84 acted through competitive inhibition. The inhibition of pancreatic α-amylase by the derivatives was non-competitive, as well as for PPC84, PPC89 and PPC101 against α-glucosidase. The results suggest that these synthetic amino acid derivatives have inhibitory potential against digestive enzymes and may be used as therapeutic agents to control metabolic disorders.

Although the vegetation of the Cerrado biome is prone to fire, plant species composition can be altered according to its severity and frequency. Furthermore, many Cerrado plant species are resistant and can adapt to frequent fires. Little is known about how plant species of Cerrado environments respond to fire and the extent to which fire is beneficial for their conservation. The present study analyzed the responses of plant species of areas of campo sujo to fire and changes in floristics and vegetation structure at different post-fire intervals. Areas with frequent fires had a greater diversity of species, while the area with 10 years without burning had lower diversity and greater DBH. The distribution of species differed among the four the studied areas. We conclude that fire was beneficial for species diversity in the studied areas of Cerrado since it was able to promote the insertion and removal of species during intervals between fires.

Circular Economy involves structural changes in traditional business models and consumers’ behaviour toward recycled products. The recycling of PET products has been increasing but there is still a gap between consumption and demand for PET packaging in Brazil. In this research, the waste reduction was examined under a projective scenario to the current ecological purposes in Brazil for reducing environmental pollution. In that manner, this paper aimed to comprehend the intention to purchase recycled PET products of Brazilian consumers. With a non-probabilistic and convenience sample, the study counts 422 participants. The method employed was based on Structural Equation Modelling and Partial Least Squares, used to test the hypotheses of causality among the variables. Results showed the perception of low quality about recycled products reduced consumers’ intention to purchase. Also, the sustainability of recycled products positively influenced the intention to purchase. In addition, recycled products presented a negative impact on the perceived safety, which could influence the purchase of recycled products in Brazil. We conclude that the image that Brazilian consumers have about recycled products positively affects the intention to purchase.

Within the field of movement sensing and sound interaction research, multi-user systems have gradually gained interest as a means to facilitate an expressive non-verbal dialogue. When tied with studies grounded in psychology and choreographic theory, we consider the qualities of interaction that foster an elevated sense of social connectedness, non-contingent to occupying one’s personal space. In reflection of the newly adopted social distancing concept, we orchestrate a technological intervention, starting with interpersonal distance and sound at the core of interaction. Materialised as a set of sensory face-masks, a novel wearable system was developed and tested in the context of a live public performance from which we obtain the user’s individual perspectives and correlate this with patterns identified in the recorded data. We identify and discuss traits of the user’s behaviour that were accredited to the system’s influence and construct 4 fundamental design considerations for physically distanced sound interaction. The study concludes with essential technical reflections, accompanied by an adaptation for a pervasive sensory intervention that’s finally deployed in an open public space.

Objective: To evaluate the effects of left intraparietal sulcus (IPS) inhibition by repetitive transcranial magnetic stimulation (rTMS) on grip force modulation (GFM) for both hands during a unimanual task. Methods: GFM induced by manual action-verb listening was evaluated for each hand in a unimanual task, and the motor-evoked potentials (MEP) were recorded for both left and right hemispheres prior to and following the left IPS inhibition. Left IPS inhibition was obtained by rTMS (5 min of 1.0 Hz, 60% of maximal stimulator output) of the international 10–20 system P3 point. Seven healthy right-handed subjects were evaluated. Results: One-way repeated measures ANOVA found that MEP amplitude and duration increased following IPS inhibition in the left hemisphere and did not change in the right hemisphere. Language-induced modulation did not change in the left hemisphere, while it was significantly attenuated in the right hemisphere. Since IPS inhibition increased the left primary motor cortex (M1) excitability, the maintenance of language-induced modulation intensity suggests it was also attenuated. Conclusion: Left IPS inhibition increased left M1 excitability without changing right M1 excitability, while attenuating the language-induced GFM for both the left and right hands.