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Preprint ESSAY | doi:10.20944/preprints201802.0131.v9

Loc Nguyen

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: expectation maximum; EM; generalized expectation maximum; GEM; EM convergence

Online: 23 November 2020 (14:25:54 CET)

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Preprint REVIEW | doi:10.20944/preprints202404.1065.v1

Anti‐Candida Phytochemicals and Isolated Compounds in Anacardiaceae Family—An Updated Review and In‐Silico Analysis

Aluisio Silva Oliveira, Josivan Regis Farias, Danielle Cristine Gomes Franco, Arthur Andre Castro da Costa, Pamela Gomes Santos, Mayara Cristina Pinto Silva, Flavia Raquel Fernandes Nascimento, Rosane Nassar Meireles Guerra

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Anacardiaceae; gallic acid; ADMET; drug-likeness; Candida albicans; antifungal

Online: 16 April 2024 (17:13:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1822.v1

Molecular Surveillance of Artemisinin-Resistant Plasmodium falciparum Parasites in Mining Areas of the Roraima Indigenous Territory in Brazil

Jacqueline De-Aguiar-Barros, Fabiana Granja, Rebecca de Abreu Fernandes, Lucas Tavarez de Queiroz, Daniel da Silva e Silva, Arthur Camurça Citó, Natalia Ketrin Almeida-de-Oliveira Mocelin, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Amazon; malaria; chemoresistance; pfk13; Guiana Shield

Online: 28 April 2024 (07:09:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1672.v1

Phenolic Monoterpenes Conversion of Conobea scoparioides Essential Oil by Hydrotalcite Synthesized from Blast-Furnace Slag

Monaliza M. Rebelo, Eloisa Helena A. Andrade, Jose Augusto M. Corrêa, José Guilherme S. Maia

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: pataqueira; thymol; essential oil composition; hydrotalcite; blast furnace slag

Online: 28 March 2024 (04:27:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0669.v1

Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits

Andrea Lauková, Ľubica Chrastinová, Valentína Focková, Iveta Plachá, Eva Bino, Ľubomira Grešáková, Zuzana Formelová, Rudolf Žitňan, Grzegorz Belzecki, Renata Miltko, Monika Pogány Simonová

Subject: Biology And Life Sciences, Life Sciences Keywords: bacteriocin; broiler rabbits; postbiotic effect; health status

Online: 12 March 2024 (13:44:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints201703.0004.v1

Purification of Polymer-Grade Fumaric Acid from Fermented Spent Sulfite Liquor

Diogo Figueira, João Cavalheiro, Bruno Sommer Ferreira

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: fumaric acid; purification; spent sulfite liquor; biorefineries

Online: 1 March 2017 (09:41:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0526.v1

Dynamic Output Feedback of Second-Order Systems: An Observer-Based Controller with LMI Design

Danielle Gontijo, José Mário Araújo, Luciano Frezzato, Fernando de Oliveira Souza

Subject: Engineering, Control And Systems Engineering Keywords: Second-order systems; Output feedback; Functional observer; LMI

Online: 9 May 2024 (09:02:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0866.v1

Mealworm Larvae Frass Exhibits a Plant Biostimulant Effect on Lettuce, Boosting Productivity beyond Just Nutrient Release or Improved Soil Properties

Meroua Foughar, Margarida Arrobas, and Manuel Ângelo Rodrigues

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: black soldier fly frass; Hermetia illucens; Tenebrio molitor; insect droppings; manuring effect

Online: 13 June 2024 (07:36:03 CEST)

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There is a need for alternatives or complements to synthetic fertilizers to enhance agricultural sustainability. Applying organic amendments can play a significant role in this. Insect droppings show high potential, though studies evaluating their agronomic value have only recently begun to emerge. This study compared black soldier fly (Hermetia illucens) and mealworm (Tenebrio molitor) larvae frass with another organic amendment (Nutrimais) derived from composting forestry, agro-industrial, and domestic waste. The experiment also included ammonium nitrate at two rates [the same as the organic amendments, 50 kg ha–1 nitrogen (N) (FullR), and half that rate (HalfR)] and an unfertilized control. The study spanned two growth cycles of lettuce (Lactuca sativa L.) grown in pots, followed by unfertilized oats (Avena sativa L.) to assess the residual effects of the fertilizing treatments. Mealworm larvae frass mineralized rapidly, with an apparent N recovery of 37.4% over the two lettuce growth cycles, indicating its high availability to soil heterotrophic microorganisms. The average dry matter yield (DMY) of lettuce was the highest among all treatments (12.8 and 9.8 g plant–1 in the first and second lettuce cycles), even compared to the FullR treatment (12.2 and 7.8 g plant–1), though without significant differences. Although mealworm larvae frass exhibited a high mineralization rate, the DMY cannot be attributed solely to N supply, as plants in the FullR treatment showed better N nutritional status. Mealworm larvae frass provided strong evidence of a plant biostimulant effect, not explained by the variables measured in this study. Black soldier fly larvae frass exhibited typical behavior of a moderately reactive organic amendment, while Nutrimais showed low reactivity, with a near-neutral mineralization/immobilization balance. The results suggest mealworm larvae frass is recommended for early maturing vegetable crops, whereas Nutrimais appears more suitable for perennial crops with low short-term nutrient requirements.

Preprint REVIEW | doi:10.20944/preprints202408.1936.v2

Artificial Intelligence in Cryo-EM Protein Particle Picking: Recent Advances and Remaining Challenges

Ashwin Dhakal, Rajan Gyawali, Liguo Wang, Jianlin Cheng

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cryo-Electron Microscopy; Protein Particle Picking; Artificial Intelligence; Machine Learning; Structural Biology

Online: 2 September 2024 (05:09:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1497.v1

Adoption of Fourth Industrial Revolution Technologies in the Construction Sector: Evidence from a Developing Country

Julia Menegon-Lopes, Luiz Carlos Pinto da Silva Filho

Subject: Engineering, Architecture, Building And Construction Keywords: Industry 4.0; Construction 4.0; New technology; Digital transformation; Developing country

Online: 23 April 2024 (11:58:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0335.v1

Cryo-EM Evidence Elucidated with AlphaFold2 Computational Prediction Provides Structural Insights into the Complex Modular Structure of GABAA Receptors

Hong Xue, Chloe Kan, Ata Ullah

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: α1β2γ2 GABAA receptor; pentameric structure; homopentamers

Online: 3 July 2024 (11:37:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0307.v1

Near-Field Coupling Effect Analysis of SMD Inductor Using 3D-EM model

GyeongRyun Choi, HyongJoo Kim, Yonggi Hong, Joosung Hwang, Euihyuk Kim, Wansoo Nah

Subject: Engineering, Electrical And Electronic Engineering Keywords: SMD inductor; loss tangent; effective permeability; Near-field coupling

Online: 5 June 2023 (10:30:01 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1341.v1

From the Gut to the Brain: Is Microbiome a New Paradigm in Parkinson’s Disease Treatment?

Cristiana Vilela, Bruna Araújo, Carla Soares-Guedes, Rita Caridade-Silva, Joana Martins-Macedo, Catarina Teixeira, Eduardo D Gomes, Cristina Prudêncio, Mónica Vieira, Fábio G. Teixeira

Subject: Biology And Life Sciences, Life Sciences Keywords: Parkinson’s Disease; Microbiome; Gut-Brain Axis

Online: 19 April 2024 (15:28:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1987.v1

Low Plasma Lipids are Associated with Relapsing and Lethal Visceral Leishmaniasis in HIV-Infected Patients

Renata V. S. Silva, Silvia R.B. Uliana, Jenicer K.U.Y. Yasunaka, Cláudio S. Veloso, Emille A Sousa, Maria Michelle L. Ferreira, Vivianne S. Carvalho, Gabriel R. Ferreira, Dorcas L. Costa, Carlos H. N. Costa

Subject: Medicine And Pharmacology, Internal Medicine Keywords: Visceral leishmaniasis; Leishmania infantum; HIV/AIDS; Lipid profile; Cachexia

Online: 30 April 2024 (11:42:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202110.0342.v2

What We Know About Kininogen Structure – Importance for Function and Perspectives from Cryo-EM

Michal Błażej Ponczek

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: kininogen; structure; AlphaFold; cryo-EM; proteins; kinin; bradykinin

Online: 1 November 2021 (13:50:19 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.1429.v1

Antimicrobial Resistance in Diverse Escherichia coli Pathotypes from Nigeria

Kenneth Nnamdi Anueyiagu, Chibuzor Gerald Agu, Uzal Umar, Bruno Silvester Lopes

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; E. coli O157:H7; Shiga-toxins; antimicrobial resistance; STEC; Diarrheagenic E. Coli; blaCTX-M; ESBL

Online: 20 August 2024 (10:54:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1032.v1

Artificial Neural Networks to Predict Metabolic Syndrome in Adolescents

Antonio Costa Júnior, Ana Karina França, Elisângela dos Santos, Victor Silveira, Alcione Santos

Subject: Public Health And Healthcare, Primary Health Care Keywords: metabolic syndrome; adolescents; screening; primary health care; artificial intelligence

Online: 15 August 2024 (02:45:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0760.v1

Transitioning from Host-Pathogen Dynamics to Social Interactions: Assessing Vaccination Strategies through the IABM Model

Marlon Nunes Gonzaga, Marcelo Martins De Oliveira, Allbens Atman

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Efficient Vaccination Strategies; Host-pathogen Interactions; Agent-Based Modeling; Complex Systems

Online: 9 July 2024 (13:56:08 CEST)

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This study presents extended Immunity Agent-Based Model (IABM) simulations to evaluate vaccination strategies in controlling the spread of infectious diseases. The application of IABM in the analysis of vaccination configurations is innovative, as a vaccinated individual can be infected depending on how their immune system acts against the invading pathogen, without a pre-established infection rate. Analysis at the microscopic level demonstrates the impact of vaccination on individual immune responses and infection outcomes, providing a more realistic representation of how the humoral response caused by vaccination affects the individual’s immune defense. At the macroscopic level, the effects of different population-wide vaccination strategies are explored, including random vaccination, targeted vaccination of specific demographic groups, and spatially focused vaccination. The results indicate that increased vaccination rates are correlated with decreased infection and mortality rates, highlighting the importance of achieving herd immunity. Furthermore, strategies focused on vulnerable populations or densely populated regions prove to be more effective in reducing disease transmission compared to randomly distributed vaccination. The results presented in this work show that vaccination strategies focused on highly crowded regions are more efficient in controlling epidemics and outbreaks. Results suggest that applying vaccination only in the densest region resulted in the suppression of infection in that region, with less intense viral spread in areas with lower population densities. Strategies focused on specific regions, in addition to being more efficient in reducing the number of infected and dead people, reduce costs related to transportation, storage, and distribution of doses compared to the random vaccination strategy. Considering that, despite scientific efforts to consolidate the use of mass vaccination, the accessibility, affordability, and acceptability of vaccines are problems that persist, investing in the study of strategies that mitigate such issues is crucial in the development and application of government policies that make immunization systems more efficient and robust.

Preprint ARTICLE | doi:10.20944/preprints202408.0690.v1

Metalloproteomics Reveals Multi-Level Stress Response in Escherichia Coli When Exposed to Arsenite

James Larson, Brett Sather, Lu Wang, Jade Westrum, Monika Tokmina-Lukaszewska, Jordan Pauley, Valérie Copié, Timothy R McDermott, Brian Bothner

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: arsenic; bacteria; metalloproteome; metal; TCA cycle; iron-sulfur cluster; molybdenum cofactor

Online: 9 August 2024 (08:30:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0089.v1

Compaction and Segregation of DNA in Escherichia coli

Conrad Louis Woldringh

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: bacterial nucleoid; phase-contrast microscopy; DNA polymer physics; protein depletion; chromosome arms; replication bubble or orisome; active and passive DNA segregation.

Online: 1 April 2024 (13:11:12 CEST)

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Theoretical and experimental approaches have been applied to study the polymer physics under-lying compaction of DNA in the bacterial nucleoid. Knowledge of the compaction mechanism is necessary to obtain a mechanistic understanding of the process of segregation of replicating chromosome arms (replichores) during the cell cycle. In the first part of this review light microscope observations are discussed that have demonstrated that the nucleoid has a lower refractive index and thus a lower density than the cytoplasm. A polymer-physical explanation for this phenomenon was given by the theory of Theo Odijk pro-posed in 1998. By assuming a phase separation between nucleoid and cytoplasm and by imposing equality of osmotic pressure and chemical potential between the two phases, a minimal energy situation is obtained in which soluble proteins are depleted from the nucleoid, thus explaining its lower density. The theory is compared with recent views for DNA compaction that are based on mere exclusion of polyribosomes from the nucleoid or on the transcriptional activity of the cell. These new views raise the question of whether they can still explain the lower refractive index or density of the nucleoid. In the second part of this review the question is discussed how DNA segregation occurs in Esche-richia coli, in the absence of the so-called active ParABS system, present in the majority of bacteria. How is entanglement prevented of nascent chromosome arms, generated at the origin in the pa-rental network of the E. coli nucleoid? The observations of the groups of Sherratt and Hansen in 2006, that the four nascent chromosome arms synthesized in the initial replication bubble, segre-gate to opposite halves of the sister nucleoids implies that extensive intermingling of daughter strands does not occur. Based on the hypothesis that leading and lagging replichores synthesized in the replication bubble, fold into micro-domains that do not intermingle, a passive "four-excluding-arms model" for segregation is proposed. This model implies that the key for seg-regation already lies in the structure of the replication bubble at the very start of DNA replication; it explains the different patterns of chromosome arms as well as the segregation distances between replicated loci as experimentally observed.

Preprint CASE REPORT | doi:10.20944/preprints202406.0287.v1

Pediatric Chikungunya-Associated Encephalitis in Itacoatiara, Brazilian Amazon: A Case Report

Samuel Benjamin Aguiar de Oliveira, Bárbara Aparecida Chaves, Maurício Teixeira Lima, Alexandre Vilhena Silva-Neto, Jady Shayenne Mota Cordeiro, Wuelton Marcelo Monteiro, Michele de Souza Bastos, Vanderson de Souza Sampaio

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Chikungunya; pediatrics; encephalitis; PRNT; case report

Online: 5 June 2024 (14:48:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1715.v1

Comparative Secretory Efficiency of Two Chitosanase Signal Peptides from Bacillus subtilis in Escherichia coli

Tae-Yang Eom, Yehui Gang, Youngdeuk Lee, Yoon-Hyeok Kang, Eunyoung Jo, Svini Dileepa Marasinghe, Heung Sik Park, Gun-Hoo Park, Chulhong Oh

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Keywords signal peptide; chitosanase; recombinant protein; expression; secretion; sec pathway; E. coli; Bacillus subtilis

Online: 25 June 2024 (05:00:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.2198.v1

Drimia maritima as a New Green Inhibitor for Al-Si Alloy, SAE Steel and Pure Al Samples in 0.5M NaCl Solution: Polarization and Electrochemical Impedance Analyses

Rodrigo S. Bonatti, Diego Costa, Giovana Padilha, Ausdinir Danilo Bortolozo, Wislei R. Osório

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: inhibitor; electrochemical impedance spectroscopy; polarization curves; Langmuir; adsorption isotherms; Al-Si alloys; low carbon steel

Online: 30 August 2024 (10:57:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0504.v2

Characterization of Aerosols Elementary Composition Emitted by Fires in the North of the Pantanal

Lucas Cardoso Ramos, Thais Costa Brunelli, Flávio César Vicentin, Leone Francisco Amorim Curado, André Matheus de Souza Lima, Fernando Gonçalves Morais, Rafael da Silva Palácios, Nicolas Neves de Oliveira, João Basso Marques

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: aerosol; AOD; EDXRF; biomass burning; black carbon

Online: 9 July 2024 (13:37:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0014.v1

Condition-Specific Competitive Effects of the Invasive Mosquito Aedes albopictus on the Resident Culex pipiens Among Different Urban Container Habitats May Explain Their Coexistence in the Field

Paul T Leisnham, Shannon L LaDeau, Megan EM Saunders, Oswaldo C Villena

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: biological invasion; interspecific competition; mosquitoes; trash; urban greenspace; West Nile virus

Online: 1 October 2021 (11:58:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1519.v1

A Multi-epitope Protein for High-Performance Serodiagnosis of Chronic Chagas Disease in Elisa and Lateral Flow Platforms

Evandro R. Dias, Andressa M. Durans, Barbara B. Succar, Luiz André L. T. Pinto, Guilherme C. Lechuga, Mariana G. Miguez, Janaina Figueira-Mansur, Ana Paula C. Argondizzo, Aline R. Bernardo, Rafaela L. Diniz, Gabriela S. Esteves, Edimilson D. Silva, Carlos M. Morel, José Borges-Pereira, Salvatore Giovanni De-Simone, Angela C. V. Junqueira, D. William Provance, Jr

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Chagas disease; Diagnostics; ELISA; Lateral Flow tests; Synthetic protein; T. cruzi

Online: 21 August 2024 (08:40:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0846.v1

Fishes and Rivers of the State of Rio de Janeiro, Southeastern Brazil: Biogeographic and Diversity Patterns in a Historically Well-Sampled Territory

Luisa. Sarmento-Soares, Felipe Vieira-Guimarães, Ronaldo F. Martins-Pinheiro

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: distribution; endemism; ichthyofauna; sampling effort; streams

Online: 11 July 2024 (09:53:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1615.v2

Adherence to Antiretroviral Therapy (ART) Among HIV-1 Patients From Sub-Saharan Africa: A Systematic Review

Pedro Almeida, Carlos Rafael, Victor Pimentel, Ana Abecasis, Cruz Sebastião, Joana Morais

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: HIV-1; Antiretroviral therapy; Treatment adherence; sub-Saharan Africa.

Online: 29 April 2024 (03:59:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1876.v1

Regulation of cysteine homeostasis and its effect on Escherichia coli sensitivity to ciprofloxacin in LB medium

Galina Smirnova, Aleksey Tyulenev, Lyubov Sutormina, Tatyana Kalashnikova, Nadezda Muzyka, Vadim Ushakov, Zoya Samoilova, Oleg Oktyabrsky

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: cysteine homeostasis; glutathione; H2S; Fur and SOS regulons; growth; respiration; antibiotics; bacterial survival

Online: 1 April 2024 (10:18:59 CEST)

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Working Paper REVIEW

Escherichia coli (E. coli) as an Indicator of Fecal Contamination in Water: A Review

Farhan Mohammad Khan

Subject: Engineering, Civil Engineering Keywords: escherichia coli; e. coli; indicator; fecal coliform bacteria; water; bacteriological analysis; fcb; classification

Online: 12 February 2020 (12:12:00 CET)

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Preprint REVIEW | doi:10.20944/preprints202406.1020.v1

Enhancing Marketing Education Through Problem-Based Learning: A Comprehensive Review

Albérico Travassos Rosário, Joana Carmo Dias

Subject: Business, Economics And Management, Business And Management Keywords: Problem-Based Learning; Marketing education; active learning; student engagement; educational innovation

Online: 14 June 2024 (11:11:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0348.v1

Virulence factors of Enteric Pathogenic Escherichia coli: A Review

Babak Pakbin, Wolfram Manuel Bruck, John W. A. Rossen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Enteric pathogenic Escherichia coli; E. coli pathotypes; Virulence factor genes

Online: 17 August 2021 (08:16:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0060.v1

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

Akamu Ewunkem, LaShunta Rodgers, Daisha Campbell, Constance Staley, Kiran Subedi, Sada Boyd, Joseph L. Graves Jr

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Escherichia coli; magnetite nanoparticles; metals; antibiotics; genomics; pleiotropy; cell morphology

Online: 1 February 2021 (15:58:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints201807.0156.v1

The Complete Genome Sequence of Escherichia coli phage Eco_BIFF

Lauren Cooper, Erica Lasek-Nesselquist, Joseph Wade

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Bacteriophage; Escherichia coli; Genome sequence

Online: 9 July 2018 (16:08:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0036.v1

Computer-Aided Bleeding Detection Algorithms for Capsule Endoscopy: A Systematic Review

Ahmmad Musha, Rehnuma Hasnat, Abdullah Al Mamun, Em Poh Ping, Tonmoy Ghosh

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Bleeding Classification; Bleeding Detection; Bleeding Recognition; Bleeding Segmentation; Capsule Endoscopy; Wireless Capsule Endoscopy

Online: 1 June 2023 (05:21:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0110.v1

Fractionation of Anaerobic Digestion Liquid Effluents Through Mechanical Treatment and Filtration

Themistoklis Sfetsas, Georgia Sarikaki, Afroditi G. Chioti, Vassilis Tziakas, Polycarpos Falaras, George Em. Romanos

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: anaerobic digestion; liquid digestate; solid fibrous digestate; fractionation; microfiltration; nanofiltration

Online: 1 June 2023 (16:36:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1564.v1

Imprecision in Yield Estimates Using Timber Volume in Forest Management Plans in the Amazon

Renato Bezerra da Silva Ribeiro, Leila Sheila Silva Lisboa, Carlos Tadeu dos Santos Dias, Afonso Henrique Moraes Oliveira, Emanuel José Gomes de Araújo, Dárlison Fernandes Carvalho de Andrade, Lucieta Guerreiro Martorano

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Community-Based Forest Management; Annual Operating Plan; Commercial Height; Tapajó; s National Forest

Online: 19 July 2024 (11:07:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.1186.v1

Uncertainties in Plant Species Niche Modelling Under Climate Change Scenarios

Isabel Passos, Albano Figueiredo, Alice Maria Almeida, Maria Margarida Ribeiro

Subject: Environmental And Earth Sciences, Ecology Keywords: climate change; plant species; range shift; &field1=keywords">species distribution models

Online: 15 July 2024 (19:48:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0140.v1

Plasmids Shape the Diverse Accessory Resistomes of Escherichia coli ST131

Arun G. Decano, Nghia Tran, Hawriya Al-Foori, Buthaina Al-Awadi, Leigh Campbell, Kevin Ellison, Louisse P. Mirabueno, Maddy Nelson, Shane Power, Genevieve Smith, Cian Smyth, Zoe Vance, Caitriona Woods, Alexander Rahm, Tim Downing

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Genome; fimbrial; plasmid; ST131; Escherichia coli; evolution; infection

Online: 8 May 2020 (09:39:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1630.v1

Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli

Liena Zaidan, Inna Novodchuk, Alexandru Nica, Alexander H.Xu, Christopher Lloyd, Reza Karimi, Joe Sanderson, Michal Bajcsy, Mustafa Yavuz

Subject: Engineering, Electrical And Electronic Engineering Keywords: Graphene; FET; Biosensor; E.coli

Online: 24 June 2024 (08:43:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.1675.v1

Molecular Epidemiology and Antimicrobial Resistance in Uropathogenic Escherichia coli in Saudi Arabia

Mateq Ali Alreshidi

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: UTI; Urinary tract infection; E. Coli; UPEC; .

Online: 23 January 2024 (10:02:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.2199.v1

A Desmethylphosphinothricin Dipeptide Derivative Effectively Inhibits Escherichia coli and Bacillus subtilis Growth

Maxim Alexey Khomutov, Fabio Giovannercole, Laura Onillon, Marija V. Demiankova, Byazilya Fedorovna Vasilieva, Arthur Ilnurovich Salikhov, Sergey Nikolaevich Kochetkov, Olga Vladimirovna Efremenkova, Alex Radievich Khomutov, Daniela De Biase

Subject: Biology And Life Sciences, Other Keywords: antibacterial; glutamate metabolism; antimicrobial resistance; central metabolism; dipeptide permeases; phosphorus-containing glutamate analogues

Online: 31 August 2023 (13:18:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1393.v1

Emotional Eating and Uncontrolled Eating as Risk Predictors for Disordered Eating Attitudes in Candidates for Bariatric Surgery Treated at a Public Hospital in the Amazon

Jeane Lorena Dias Kikuchi, Manuela Maria de Lima Carvalhal, Millena Borges Inete, Yasmym Dannielle do Espírito Santo Souza, Tainá Martins Moraes, Rafaela Lorena Viana Costa, Rafaelle Dias Gabbay, Carla Cristina Paiva Paracampo, Daniela Lopes Gomes

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: obesity; bariatric surgery; eating behavior; eating disorders

Online: 22 April 2024 (10:15:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0338.v1

A Synthetic Pathway for the Production of Benzylsuccinate in Escherichia coli

Johanna Mock, Karola Schühle, Uwe Linne, Marco Mock, Johann Heider

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: anaerobic toluene degradation; reverse -oxidation; synthetic pathway; transport; CoA ligase; CoA-transferase; benzylsuccinate

Online: 6 December 2023 (07:16:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1179.v2

Use of Soil Moisture as an Indicator for Climate Change, Using the SUPer System

Josicleda Galvincio, Rodrigo Miranda, Gabrielly Luz

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Monitoring; modeling; natural disaster; water balance; SWAT model

Online: 4 April 2024 (12:59:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0385.v1

Differential Impact of Hexuronate Regulators ExuR and UxuR on the Escherichia coli Proteome

Tatiana A. Bessonova, Maria S. Fando, Olga S. Kostareva, Maria N. Tutukina, Olga N. Ozoline, Mikhail S. Gelfand, Alexey D. Nikulin, Svetlana V. Tishchenko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: UxuR; ExuR; Ashwell pathway; uxuAB; fliC; proteome; motility; biofilm formation

Online: 28 June 2022 (10:42:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1839.v1

Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in Escherichia coli

Isidoro López, Fátima Otero, María del Carmen Fernández, Germán Bou, Jaime Gosálvez, José Luis Fernández García

Subject: Medicine And Pharmacology, Other Keywords: antibiotic combination; bacterial nucleoid; DNA fragments; rapid antibiogram; quinolone; beta-lactam

Online: 28 May 2024 (10:47:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0998.v1

Whole Genome Sequencing of Human and Porcine Escherichia coli Isolates on a Commercial Pig Farm in South Africa

Wilhelmina Strasheim, Michelle Lowe, Anthony M Smith, Eric MC Etter, Olga Perovic

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Escherichia coli; pigs; close human contacts; One Health; antibiotic resistance; whole genome sequencing; virulence factors; core genome MLST and hierarchical clustering; sequence type complex 10; South Africa

Online: 15 May 2024 (04:32:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0974.v1

Reliability Estimation for Industrial Equipment Using EM Algorithm with Censored Data: A Case Study on Centrifugal Pumps in an Oil Refinery

José Silva, Paulo Vaz, Pedro Martins, Luís Ferreira

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: Reliability estimation; EM algorithm; Censored data; Weibull distribution; Industrial equipment; Maintenance optimization; Failure analysis; Proactive maintenance

Online: 14 June 2023 (07:50:43 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202402.0438.v1

A Successful Approach in Diagnosing Shiga - Toxin Producing Escherichia coli -Induced Colitis

Violeta Melinte, Cristina Maria Văcăroiu, Adelina Maria Radu, Miriana Ioana Cismaru, Amalia Loredana Călinoiu, Adina Rusu, Anca Monica Oprescu Macovei, Mihai Ciocîrlan, Elena Cristina Tianu, Luminița Mîrzan, Daniela Elena Mihăilă, Ioana Veronica Trandafir, Claudia Sorina Bugeac Mitacu, Valeriu Gheorghiță

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: STEC, bloody diarrhoea, ischemic colitis, E coli

Online: 7 February 2024 (11:54:06 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202401.1639.v1

Exploring Hypericin as a Potential Modulator of Key Targets in Escherichia coli

Ivan Vito Ferrari

Subject: Biology And Life Sciences, Life Sciences Keywords: E. coli; Hypericin; Docking analysis; Mcule Server

Online: 23 January 2024 (06:45:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0352.v1

Effect of Bacteriophages against Biofilms of Escherichia coli on Food Handling Surfaces

Ana Brás, Márcia Braz, Inês Martinho, João Duarte, Carla Pereira, Adelaide Almeida

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Escherichia coli; bacterial biofilm; bacteriophages; surface decontamination; plastic; stainless steel; food safety

Online: 4 January 2024 (11:13:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0340.v1

Trans-Kingdom Conjugation Within Solid Media from Escherichia coli to Saccharomyces cerevisiae

Maximillian P.M. Soltysiak, Rebecca S. Meaney, Samir Hamadache, Preetam Janakirama, David R. Edgell, Bogumil J. Karas

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: conjugation; solid media; saccharomyces cerevisiae; trans-kingdom; escherichia coli; pta-mob; yeast assembly

Online: 30 September 2019 (05:08:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201812.0236.v2

Specificity of Escherichia coli Heat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography

Julie E. Heggelund, Joel B. Heim, Gregor Bajc, Vesna Hodnik, Gregor Anderluh, Ute Krengel

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: bacterial toxin; cholera toxin; Escherichia coli heat-labile enterotoxin; lectin; N-acetyllactosamine binding; neutral glycosphingolipids; protein-carbohydrate interactions; surface plasmon resonance spectroscopy; X-ray crystal structure

Online: 28 January 2019 (15:23:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1186.v1

Molecular Characterization and Prevalence of Antimicrobial Resistant Escherichia coli Isolates Derived From Environmental Habitats

Chrysa Dioli, Olga Pappa, Eirini Siatravani, Spyridoula Bratakou, Apostolos Tatsiopoulos, Panagiota Giakkoupi, Vivi Miriagou, Apostolos Beloukas

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: E. coli; environment; antibiotic resistance; antibiotic resistance genes

Online: 17 May 2023 (04:43:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201910.0372.v1

Preparation and Application of a Xylan-Based Antibacterial Additive Agent against Escherichia Coli Bacteria

Guibin Xu, Tao Song, Bei He, Minmin Chang, Junli Ren

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: antimicrobial additive agent; cationic-xylan; Escherichia coli; mechanical properties; paper products; PHGH; thermal stability

Online: 31 October 2019 (10:22:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0242.v1

Influence of Dose, Particle Size &Amp; Concentration on Dermal Penetration Efficacy of Curcumin

Em-on Chaiprateep, Sabrina Wiemann, Ralph W Eckert, Christian S. Raab, Soma Sengupta, Cornelia M. Keck

Subject: Medicine And Pharmacology, Dermatology Keywords: nanocrystals; curcumin; aqueous meniscus; dermal penetration; ex-vivo model; porcine skin

Online: 5 October 2023 (02:10:32 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202408.0913.v1

Rugby Union Match Injury Surveillance in Portuguese Top-Tier Competition

Nuno Pinto de Sousa Lopes, António Miguel Cruz-Ferreira, Diana Freitas Torres Lima, Marta Amaral Silva, Luiz Miguel Santiago

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: Sports medicine; injury surveillance; Rugby; injury prevention; incidence

Online: 13 August 2024 (11:09:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0206.v1

Utilizing Olive By-Products in the Diet of Bísaro Pigs: Enhancing the Value of the Breed's Dry-Cured Products

Ana Leite, Lia Vasconcelos, Sergio Lopez, Divanildo Outor Monteiro, Vitor Pinheiro, Sandra Rodrigues, Alfredo Teixeira

Subject: Biology And Life Sciences, Food Science And Technology Keywords: native Bí; saro pig; dry-cured product; olive cake; valorization

Online: 2 July 2024 (10:26:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1855.v1

Antimicrobial Susceptibility of Uropathogenic Escherichia coli with Analysis of Impact of Socio-Economic Factors in Low, Medium and High-Income Countries

Meher Rizvi, Maria Khan, Amina AL Jardani, Ujjala Ghoshal, Zaaima AL Jabri, Mohammad Ahanjan, Azza AL Mamari, Nawal AL Shizawi, Hiba Sami, Abdullah Balkhair, Adila Shaukat, Alwarith Nasser Salem Alkharusi, Abdulrahman Almahrouqi, Afreenish Hassan, Ajay Kumar, Amal Malehi, Amina Gul, Ashima Singh, Asma Said Hamed AL Shidhani, Areena Hoda Siddiqui, Aruna Poojary, Azza AL Rashdi, Bradley Langford, Dmytro Stepanskyi, Abbas Dolatabadi, Aisha Al Mufraji AL Mufraji, Elham Said Ahmed AL Risi, Garima Kapoor, Isabella Princess, Hatim Ali Eltahir, Hilal Al Sidairi, Kaitlyn Dougherty, Katia Iskandar, Ken Masters, Ibrahim Khalaf Hamdan AL Busaidi, Laila AL Yazidi, Mahfuza Nasrin, Mahmood Salim AL Subhi, Mallika Sengupta Mallika Sengupta, Manisha Khandait, Nada Khalfan AL Tamtami, Nada Khafan Alsiyabi, Nawal AL Kindi, Nazla Musthafa Luthfee, Neha Shreshtha, Nihal Mohamed Amur AL Riyami, Noora Hilal Ali AL Busaidi, Nupur Goel, Oksana Ishchenko, Omnia Mohamed Elnabawy Ahmed Taher, Pragnya Paramita Jena, Rajni Ekadashi, Razan Zadjali, Reba Kanungo, Sara Abolghasemi, Salima Al-maqbali, Sanjay Jaju, Sayantan Banerjee, Shadma Yaqoob, Shahnaz Sali, Shafqat Husnain Khan, Shalini Malhotra, Stephen Hughes, Sundas Shaukat, Syed Mohammed Atif, Vrushali Vishwas Patwardhan, Victorien Dougnon, Wahid Khan, Walid Wali, Zakariya AL Muharrmi, Zeeshana Basit, Jasashree Choudhury, Rachita Pravalina, Sanjeev Singh, Sarman Singh, Neelam Taneja, Raman Sardana, Pawan Kapoor, Rajeev Soman, Rashid Al Abri

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: community acquired UTI; Escherichia coli; antimicrobial susceptibility; Indian subcontinent; MENA; GDP; temperature

Online: 24 September 2024 (12:32:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0101.v1

Measuring the influence of key management decisions on the nitrogen nutritional status of annual ryegrass-based forage crops

Luís Silva, Sofia Barbosa, Fernando Cebola Lidon, José Santos-Silva, Luís Alcino Conceição

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: critical nitrogen concentration; data-driven approach; crop yield; site-specific analysis; sustainability; variable rate fertilisation.

Online: 1 July 2024 (12:55:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0606.v1

A Common Polymorphism in RNASE6 Impacts Its Antimicrobial Activity Toward Uropathogenic Escherichia coli

Raul Anguita, Guillem Prats-Ejarque, Mohammed Moussaoui, Brian Becknell, Ester Boix

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: RNase; RNase 6; RNase k6; single nucleotide polymorphisms; antimicrobial peptides; urinary tract infections; uropathogenic Escherichia coli

Online: 9 November 2023 (07:22:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0869.v1

A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm

Xin Jiang, Xuewei Fan

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: donkey; Escherichia coli; antibiotic resistant

Online: 10 August 2023 (13:21:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0153.v2

Antimicrobial Resistance Pattern of Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh

Mst. Sonia Parvin, Sudipta Talukder, Md. Yamin Ali, Emdadul Haque Chowdhury, Md. Tanvir Rahman, Md. Taohidul Islam

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; antimicrobial resistance; ESBL; MDR; frozen chicken meat; Bangladesh

Online: 27 May 2020 (08:31:20 CEST)

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Escherichia coli is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing E. coli (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of E. coli, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in E. coli. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of E. coli were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for E. coli, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3–5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim–sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the blaTEM gene. However, the blaSHV and blaCTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the blaCTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors.

Preprint COMMUNICATION | doi:10.20944/preprints202206.0374.v1

Highly Virulent and Multidrug-Resistant Escherichia Coli Sequence Type 58 from a Sausage in Germany

Elias Eger, Marielle Domke, Stefan E. Heiden, Madeleine Paditz, Veronika Balau, Christiane Huxdorff, Dirk Zimmermann, Timo Homeier-Bachmann, Katharina Schaufler

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: antimicrobial resistance; CTX-M-1; Enterobactericeae,; Escherichia coli; extended-spectrum β-lactamase; food safety; IncI1; next-generation sequencing; One Health; virulence

Online: 28 June 2022 (04:18:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0340.v1

Prevalence, Phylogroups, Antimicrobial Susceptibility and GE-Netic Diversity of Escherichia coli Isolates From Food Products

Babak Pakbin, Samaneh Allahyari, Zahra Amani, Wolfram Manuel Bruck, Razzagh Mahmoudi, Amir Peymani

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; Antimicrobial resistance; Food samples; Phylogenetic group; Genetic diversity

Online: 16 August 2021 (13:35:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0422.v1

Antibiotic-Resistant Escherichia Coli and Sequence Type 131 in Fecal Colonization in Dogs in Taiwan

Jenn-Wei Chen, Han Hsiang Huang, Szu-Min Chang, Joy Scaria, Yu-Lung Chiu, Chih-Ming Chen, Wen-Chien Ko, Jiun-Ling Wang

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: dog; ST131; ESBL; fecal colonization; Escherichia coli

Online: 18 September 2020 (07:15:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1083.v1

The Role of Propionate-Induced Rearrangement of Membrane Proteins in the Formation of the Virulent Phenotype of Crohn’s Disease-Associated Adherent-Invasive Escherichia coli

Olga Vladimir Pobeguts, Maria Alexey Galyamina, Elena Vladimir Mikhalchik, Sergey Igor Kovalchuk, Igor Pavel Smirnov, Alena Vyacheslav Lee, Lyubov Yuriy Filatova, Kirill Vitaly Sikamov, Oleg Mikhayl Panasenko, Alexey Yuriy Gorbachev

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Crohn’ s disease; Crohn’ s disease-associated adherent-invasive Escherichia coli (AIEC); propionic acid; neutrophiles; membrane proteins; 2D electrophoresis; LC-MS analysis

Online: 16 August 2024 (03:45:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2203.v1

Disinfection of Digestate Effluents Using Photocatalytic Nano-Filtration

Afroditi G. Chioti, Georgia Sarikaki, Vasiliki Tsioni, Eleni Kostopoulou, George Em. Romanos, Polycarpos Falaras, Themistoklis Sfetsas

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: disinfection; indicator pathogens; TiO2 photocatalysis; photocatalytic nanofiltration reactor; membrane filtration; anaerobic digestion; liquid digestate

Online: 31 May 2023 (08:36:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1551.v1

A Genetically Engineered Escherichia coli Strain for Potential Utilization in Fungal Smut Disease Control

Guobing Cui, Xinping Bi, Shan Lu, Zide Jiang, Yizhen Deng

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: filamentous growth; jasmonic acid carboxyl methyl transferase (JMT); methyl jasmonate (MeJA); pathogenicity; Sporisorium scitamineum; sugarcane smut

Online: 23 May 2023 (03:41:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202202.0321.v1

Enteroinvasive Escherichia Coli is Inefficient to Escape from Autophagy during Caco-2 Cellular Infection

Silvia Yumi Bando, Ana Carolina Ramos Moreno, Hadassa Cristhina de Azevedo Soares dos Santos, Fernanda Bernardi Bertonha, Lucas Gonçalves Ferreira, Marina Baquerizo Martinez, Carlos Alberto Moreira-Filho

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Enteroinvasive Escherichia coli; Shigella flexneri; Caco-2 cells; icsB; autophagy; WGCNA; gene co-expression network

Online: 25 February 2022 (03:16:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202009.0381.v1

Automated Conditional Screening of Escherichia Coli Knockout Mutants in Parallel Adaptive Fed-Batch Cultivations

Sebastian Hans, Benjamin Haby, Niels Krausch, Barz Tilman, Peter Neubauer, Mariano Nicolas Cruz-Bournazou

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: high throughput screening; rapid phenotyping; model-based experimental design; Escherichia coli; automated bioprocess development

Online: 17 September 2020 (07:34:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1170.v1

Antibacterial Activity of Superhydrophobic-SiO₂ Coatings to Inhibit the Growth of Escherichia coli and Staphylococcus aureus

Betania Sánchez-Santamaria, Delfino Cornejo-Monroy, Imelda Olivas-Armendáriz, José Saúl Arias-Cerón, Alfredo Villanueva-Montellano, Elsa Ordoñez-Casanova, Jose Omar Dávalos-Ramírez, Erwin Adán Martínez-Gómez, Jesús Manuel Jaquez-Muñoz

Subject: Chemistry And Materials Science, Nanotechnology Keywords: SiO₂ NPs; superhydrophobic coatings; SH-SiO₂ coating; PFDTES modification; antibacterial and bacteriostatic evaluation; biofilm prevention; nanotechnology; growth inhibition

Online: 20 August 2024 (03:58:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0066.v1

Design of the EPIGENEC Study: Assessing the EPIdemiology and GENetics of Escherichia coli in the Netherlands

Denise van Hout, Tess D. Verschuuren, Patricia C.J. Bruijning-Verhagen, Thijs Bosch, E. Ascelijn Reuland, Ad C. Fluit, Anita C. Schürch, Rob J.L. Willems, Sabine C. de Greeff, Annemarie van ’t Veen, Jan A.J.W. Kluytmans, Marc J.M. Bonten

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; antimicrobial resistance; infection; molecular epidemiology

Online: 7 February 2019 (09:15:44 CET)

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Background: Infections caused by E. coli cause considerable disease burden and range from frequently occurring and relatively innocent urinary tract infection (UTI) to severe bloodstream infection (BSI). The incidence of infections caused by ESBL-producing E. coli (ESBL-PEc) is increasing, justifying surveillance and development of preventive strategies in several domains. Faecal carriage is universal and believed to be the most important reservoir for E. coli from which infections can originate. It is currently unknown to what extent Dutch E. coli carriage strains in the community reflect isolates causing disease. In this study, we will perform comparative genomics to infer the population structures of human-derived ESBL-PEc from community- and hospital-acquired infections and from community-based faecal carriage samples in the Netherlands. Furthermore, we will describe the molecular epidemiology of E. coli isolates causing invasive disease (BSI). Methods: This study uses four different microbiological data sources: 1) ESBL-PEc from patients with community-acquired UTI tested in primary care between May and November 2017, 2) ESBL-PEc from urine cultures obtained from patients hospitalized between January 2014 and December 2016, 3) E. coli from blood cultures obtained from patients hospitalized between January 2014 and December 2016, and 4) ESBL-PEc from faecal samples collected in a national population- prevalence study performed between January 2014 and January 2017. Clinical epidemiological data was collected from all patients and all isolates were subjected to whole genome sequencing. Discussion: The EPIGENEC study (EPIdemiology and GENetics of E. coli) will describe the molecular epidemiology of E. coli BSI and assess the genomic population structure of ESBL-PEc strains from community-acquired and nosocomial infections, and of ESBL-PEc reflecting community-based faecal carriage. Information from these studies may assist in optimizing surveillance strategies and determining targets and potential impact of future new preventive measures.

Preprint REVIEW | doi:10.20944/preprints202009.0709.v1

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in ‘No Antibiotics Ever’ Broiler Production and Potential Solutions

Courtney Fancher, Li Zhang, Aaron Kiess, Pratima Adhikari, Thu Dinh, Anuraj Theradiyil Sukumaran

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Escherichia coli; Clostridium perfringens; broiler; antibiotic-free; production; chicken

Online: 29 September 2020 (12:46:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0828.v1

Scipion-EM-ProDy: A Graphical Interface for the ProDy Python Package within the Scipion Workflow Engine enabling Integration of Databases, Simulations and Cryo-Electron Microscopy Image Processing

James Michael Krieger, Carlos Oscar S. Sorzano, Jose Maria Carazo

Subject: Biology And Life Sciences, Biophysics Keywords: global protein dynamics; software integration workflows; cryo-electron microscopy; normal mode analysis; elastic network models; ensemble analysis; principal component analysis; hybrid simulations

Online: 10 August 2023 (10:52:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0329.v1

Characterization and Preclinical In Silico Safety Assessment of Three Virulent Bacteriophages Targeting Carbapenem-Resistant Uropathogenic Escherichia coli

Gunaraj Dhungana, Roshan Nepal, Ghais Houtak, George Spyro Bouras, Sarah Vreugde, Rajani Malla

Subject: Biology And Life Sciences, Virology Keywords: Bacteriophage; myovirus; Myroviridae; phage; phage therapy; lytic phage; virulent phage; carbapenem-resistant Enterobacteriaceae; antimicrobial resistance; AMR; alternative medicine

Online: 6 November 2023 (11:22:33 CET)

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Escherichia coli causes several infections like intestinal diarrhea, urinary tract infections, and pneumonia, which are difficult to treat due to the emergence of antimicrobial resistance. The World Health Organisation (WHO) declared carbapenem-resistant E. coli a critical pathogen and called for the development of new antimicrobial agents. Phage therapy, a century-old technique, has been revitalised recently with many successful applications against multi-drug resistant infections. As such, phage therapy is considered one of the most effective alternatives or adjuvants to antibiotics and is also effective in treating multidrug-resistant (MDR) bacterial infections. However, few phages that can kill pathogenic E. coli strains from Southeast Asia have been studied at a molecular level, although the burden of MDR, particularly carbapenem resistance, is high in this region. In this study, three closely related obligately virulent Escherichia phages (øEc_Makalu_001, øEc_Makalu_002, and øEc_Makalu_003) that could infect uropathogenic E. coli were isolated from sewage samples and characterized using morphological as well as sequence-based analysis. Further, a comparative genomic and phylogenetic analysis was done to predict the functional genes and evolutionary relationships. Electron microscopy and genome-based classification revealed that all three phages belonged Krischvirus genus of Straboviridae family. One-step growth curve showed that the latent period of the phages ranged from 15-20 min, the outbreak period was about 50 min, and the burst size ranged between 74-127 PFU/bacterium. Moreover, the phages could tolerate a pH range of 6 to 9 and a temperature range of 25-37°C for up to 180 minutes without significant loss of phage viability. Spotting the purified phages in different clinical isolates showed a broad host-range of isolated Escherichia phages and could lyse up to 30% of the tested pathogenic clinical isolates, including an intra-genus isolate of Klebsiella pneumoniae. The genome organization of all three phages was almost identical and similar to T4-like phage with a full-length genome of ~163 kb. Around 284 coding sequences (CDS) were predicted in each phage, of which only 40% were functional with an average gene length of 951 bp. The coding density in all phages was approximately 95%, while the gene density was 1.73 gene/kbp. Putative lysin, holin, endolysin, and spanin genes were found in the genomes of all three phages, resembling that of T4-like phages implying their phylogenetic relatedness. Furthermore, various in silico sequence analysis tools confirmed that all three phages were strictly virulent with functional lysis modules but devoid of any known virulence or toxin genes, integron-associated genes and antimicrobial-resistance (AMR) genes. In summary, pre-clinical experimental analysis and exploration of genomic features suggest that all three phages may be suitable candidates for therapeutic applications against uropathogenic E. coli.

Preprint ARTICLE | doi:10.20944/preprints202306.1904.v1

Effectiveness of Bacteriophages Against Biofilm-forming Shiga-toxigenic Escherichia coli In-vitro and on Food-Contact Surfaces

Divya Jaroni, Pushpinder Kaur Litt, Punya Bule, Kaylee Marie Rumbaugh

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Bacteriophages; Shiga-toxigenic E. coli; biocontrol; biofilms; food-contact surfaces

Online: 27 June 2023 (12:36:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202012.0679.v1

Prevalence of Antimicrobial Resistance (AMR) Salmonella spp. and Escherichia coli Isolated From Broilers in East Coast Malaysia

Nor Fadhilah Kamaruzzaman, Shamsaldeen Saeed

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Antimicrobial resistance; Salmonella species; E.coli; Broiler chickens; Malaysia

Online: 28 December 2020 (10:49:17 CET)

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Abstract:Salmonella species (spp) and Escherichia coli (E. coli) are the most common infectious pathogens in poultry. Antimicrobials were given either for the treatment or growth promoters that can increase the possibility of emergence of bacterial resistance towards antimicrobials. The aim of this study was to determine the prevalence of antimicrobial resistant (AMR) Salmonella spp and E. coli isolated from a sample of broiler farms in East Coast Malaysia from 2018-2019. A total of 384 cloacal swabs were collected from broilers farms in Kelantan, Terengganu, and Pahang. The bacteria were isolated and confirmed by bacteriological and serological methods. Following that, confirmed isolates were subjected to antimicrobial susceptibility test. Salmonella spp and E. coli were recovered from the cloacal swabs samples with the overall prevalence of 6.5% and 51.8% respectively. In Kelantan, Terengganu and Pahang, the prevalence of Salmonella spp were 7%, 6.5% and 5.8% respectively, while the prevalence for E. coli were 50%, 48.3% and 58% respectively. Salmonella spp and E. coli displayed resistance towards the following antimicrobials: erythromycin (100% for both pathogens), chloramphenicol (76.2%, 84.5%), tetracycline (62%, 94.6%), ampicillin (47.7%, 87%), sulfamethoxazole/trimethoprim (42.9%, 83.3%), ciprofloxacin (4.8%, 23.8%), nalidixic acid (9.6%, 60.7%), streptomycin (19%,66%), and kanamycin (28.6%,57%), cephalotin (0%, 11%), gentamicin (0%, 20.2%) respectively. No resistance were recorded towards colistin for both pathogens. Multidrug resistance (MDR) was recorded in 82% of Salmonella spp and 100% of E. coli. These findings demonstrate the high prevalence of MDR Salmonella spp. and E. coli in broiler farms in East coast Malaysia. This could be attributed to the excessive use of antimicrobial agents by the poultry farm owners. Enhanced control measures and a strong monitoring system should be urgently implemented to reduce the emergence of antimicrobial resistance that is harmful to public health.

Preprint COMMUNICATION | doi:10.20944/preprints202307.2123.v1

Biosynthesis of Glucaric Acid by Recombinant Strain of Escherichia coli Expressing Two Different Urinate Dehydrogenases

Xinchao Yang, Linlin Niu, Chunjiang Ye, Yuanxiu Wang, Yuehui Liu, Fang Wang, Sun Naxin

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Urinate dehydrogenases; pETDuet-2×Ud; D-Glucaric acid; Glucuronic acid; SDS-PAGE

Online: 1 August 2023 (02:45:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1800.v1

Comparison of Antibiotic Resistance of Escherichia coli Populations from Water or Sediment in Rivers Environments

Skof Aline, Michael Koller, Rita Baumert, Jürgen Hautz, Fritz Michael Treiber, Clemens Kittinger, Gernot Zarfel

Subject: Biology And Life Sciences, Life Sciences Keywords: biofilm; ESBL; KPC-2; wastewater treatment plant; phenotyping

Online: 26 June 2023 (11:14:25 CEST)

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The detection of antibiotic-resistant facultative pathogenic bacteria in surface waters is common in the present day. However, there is limited understanding of the factors that influence the spread and stabilization of resistance in this habitat, particularly regarding the role of biofilms. Despite the perceived differences between sediments, biofilms, and open water, their potential contribution to the long-term maintenance of resistances remains unclear. In this study, we investigated Escherichia coli isolates obtained from the Mur and Drava rivers in Austria. Samples were collected from both the water column and sediment at two locations per river: upstream and downstream of an urban area, including a sewage treatment plant. The iso-lates were subjected to antimicrobial susceptibility testing against 21 antibiotics belonging to seven distinct classes. Additionally, any isolates exhibiting an extended-spectrum beta-lactamase (ESBL) or carbapenemase phenotype were further analyzed for the presence of specific antimi-crobial resistance genes. E. coli isolates obtained from the two rivers exhibited resistance to at least one of the tested anti-biotics, with rates of 25.83% and 23.66% respectively. The most prevalent antimicrobial re-sistances observed were towards ampicillin, amoxicillin-clavulanic acid, tetracycline, and na-lidixic acid. Surprisingly, there was a similar proportion of resistant bacteria observed in both open water and sediment samples, contrary to expectations. The difference in resistance levels between samples collected upstream and downstream of the cities was minimal. Out of the total isolates, 13 E. coli were found to carry ESBL genes with one isolate carrying the gene for KPC-2 carbapenemase. There were no significant differences between the biofilm (sediment) and open water samples in the occurrence of antimicrobial resistance. Even the difference between the samples upstream and downstream of the cities and their wastewater treatment plants was minor. There seems to be no evidence of a large adaptive effect in this analysed biofilm population of E. coli. The un-typical phenotype of the KPC-producing and some of the ESBL-producing E. coli from this study could, however, be an indication that such adaptation effects generally exist in water.

Preprint REVIEW | doi:10.20944/preprints202402.1579.v1

Overview of Ethnobotanical-Pharmacological Studies carried out on Medicinal Plants from the Serra da Estrela Natural Park

Radhia Aitfella Lahlou, Filomena Carvalho, Maria João Pereira, João Lopes, Luís R. Silva

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Natural Park of Serra da Estrela; Botanical diversity; Medicinal plants; Diabetes prevention and treatment; Pharmacological applications; chemical composition.

Online: 27 February 2024 (16:02:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0748.v1

Existence of Heteroclinic Solutions in Nonlinear Differential Equations of the Second-Order Incorporating Generalized Impulse Effects with the Possibility of Application to Bird Population Growth

Robert de Sousa, Marco António de Sales Monteiro Fernandes

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Heteroclinic solutions; L1-Carathé; odory functions and sequences; equiconvergence at infinity and at the impulsive points

Online: 9 July 2024 (13:38:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0274.v1

Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance

Nikoleta Iosifidou, Eleni Anagnostopoulou, Maria Botou, Eirini Kalfa, Ekaterini Tatsaki, Stathis Frillingos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: gemcitabine; chemoresistance; gamma-proteobacteria; Escherichia coli K-12; nucleoside transporters

Online: 5 June 2024 (12:57:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1396.v1

Positive Assessment of an Autogenous Vaccine Against Pathogenic Escherichia coli in Broiler Breeders and their Progeny in Morocco

Lhoussaine Oubouyahia, Amal Essalah-Bennani, Ouafaa Fassi Fihri, Eric Badin, Hanan Balil, Eric Thibault, Hubert Gantelet, Saâdia Nassik

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: APEC; colibacillosis; antimicrobial resistance; autogenous vaccine; poultry integration; Morocco

Online: 23 October 2023 (11:00:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1075.v1

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Siyanda S. Ngema, Solomuzi H. Khumalo, Michael C. Ojo, Ofentse J. Pooe, Tsolanku Sidney Maliehe, Albertus K. Basson, Evelyn Madoroba

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Nocardiopsis dassonvillei SOD(B)ST2SA2; Antibacterial activity; Molecular docking; Listeria monocytogenes

Online: 15 August 2023 (11:45:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0239.v1

Bioengineering of Escherichia coli Nissle 1917 for Overproduction and Excretion of Spermidine, a Key Metabolite in Human Health

Clement Caffaratti, Caroline Plazy, Valérie Cunin, Bertrand Toussaint, Audrey Le Gouellec

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Microbiota; Immunity; Spermidine; Metabolic engineering; Probiotics; Live Biotherapeutic Product; Metabolomics

Online: 17 October 2022 (12:09:41 CEST)

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Working Paper REVIEW

DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication Forks in Escherichia coli.

Piero R. Bianco

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: RecG; SSB; stalled DNA replication fork; DNA repair; DNA replication; helicase; atomic force microscopy; OB-fold; SH3 domain; PXXP motif

Online: 2 April 2020 (04:26:52 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints201909.0150.v1

First Report and Comparative Genomics Analysis of a bla_OXA-244–Harbouring Escherichia coli Isolate Recovered in American Continent

Deisy J Abril, Ingrid Gisell Bustos Moya, Ricaurte Alejandro Marquez-Ortiz, Diego Fernando Josa Montero, Zayda Lorena Corredor Rozo, Isabel Torres Molina, Natasha Vanegas Gómez, Javier Escobar-Perez

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: blaOXA-244; Escherichia coli; carbapenems; resistance; Colombia

Online: 15 September 2019 (06:19:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201907.0137.v1

Assessment of Probiotic Potential and Antagonistic Characteristics of Bifidobacterium bifidum Isolated from Infant Feces against Enteropathogenic Escherichia coli

Muhammad Ashraf, Rehana Kanwal, Iahtasham Khan, Sadia Munir, Rizwan Aslam, Sultan Ali, Ali Abbas, Mubarik Ali, Ghazanfar Abbas

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; Bifidobacterium bifidum; probiotics; enteropathogens

Online: 10 July 2019 (06:04:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0132.v1

Isolation and Characterisation of Bacteriophages with Lytic Activity Against Virulent Escherichia coli O157:H7: Potential Bio-Control Agents

Collins Njie Ateba, Muyiwa Ajoke Akindolire

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Bacteriophages; Bio-control; E. coli O157:H7; Podoviridae; TEM, safety

Online: 14 January 2019 (11:18:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints201703.0087.v1

Inhibition of Zika Virus by Marine Algae

Claudio Cirne-Santos, Caroline De Souza Barros, Caio Cesar Richter Nogueira, Renata Mendonça Campos, Valéria Teixeira, Davis Ferreira, Izabel Christina Nunes De Palmer Paixão

Subject: Biology And Life Sciences, Virology Keywords: Zika Virus, Antiviral, Marine Algae

Online: 14 March 2017 (18:34:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.1226.v1

Digital PCR Validation for Characterization of Quantitative Reference Material of Escherichia coli O157:H7 Genomic DNA

Claudia Patricia Tere-Peña, Martha Nancy Calderon-Ozuna, John Emerson Leguizamón

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: E. coli O157:H7; Reference material; Method validation; digital PCR

Online: 18 June 2024 (11:03:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0065.v1

Phylogenetically Diverse Escherichia Coli Strains From Chicken Co-Harbour Multiple Carbapenemase Encoding Genes (blaNDM-blaOXA-blaIMP)

Erkihun Aklilu, Azian Harun, Kirnpal Kaur Banga Singh, Shamsaldeen Ibrahim, Nor Fadhilah Kamaruzzaman

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Carbapenem resistant Enterobacteriaceae (CRE); E.coli; Antimicrobial Resistance; Multidrug resistance; Phylogenetic diversity; chicken; food animals; Antimicrobials

Online: 2 November 2020 (17:14:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202009.0494.v1

Multidrug Resistant Escherichia coli Harboring Extended-spectrum β-lactamase-encoding Genes Isolated from Clinically-healthy Pigs

Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Sinwat Nuananong, Rungtip Chuanchuen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: ESBL; Escherichia coli; Pigs; Thailand

Online: 21 September 2020 (04:26:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0589.v1

Adsorption of Hydrogen Sulphide on Activated Carbon Materials Derived from the Solid Fibrous Digestate

Evangelia Choleva, Anastasios Mitsopoulos, Georgia Dimitropoulou, George Em. Romanos, Evangelos P. Kouvelos, Georgios Pilatos, Stylianos D. Stefanidis, Angelos Lappas, Themistoklis Sfetsas

Subject: Environmental And Earth Sciences, Other Keywords: activated carbon; hydrogen sulfide; biogas; physical adsorption; micropores; biogas; solid fibrous digestate; carbon dioxide; mesopores

Online: 8 June 2023 (04:31:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1546.v1

Perception of Individualized Nursing Care in Acute Medical and Perioperative Settings: A Cross-Sectional Study

Ana Ramos, Sara Pires, Eunice Sá, Idalina Gomes, Elisabete Alves, César Fonseca, Anabela Coelho

Subject: Public Health And Healthcare, Nursing Keywords: Patient individualization; Nursing care; Acute care; Perioperative care

Online: 19 September 2024 (15:35:02 CEST)

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Abstract: Individualized nursing care allows a systematic assessment and intervention, considers the patient's preferences, values, and context, and contributes to a positive care trajectory. However, its operationalization has proven to be challenging. This research aims to determine which items of nursing care individualization are most integrated into nurses' clinical practice, and those that are less included. A cross-sectional study was conducted on 122 eligible and registered nurses at a Hospital Center, in Ophthalmology (operating room and inpatient ward), Cardiology Service, Internal Medicine, and Medical Emergency Unit, in Portugal. The Indi-vidualized Care Scale nursing version (ICS-Nurse) was used for the assessment, including three sub-dimensions: clinical situation, personal life situation, and decisional control over care-related decisions. Analyses were performed using Cronbach's Alpha and Principal Component Analysis to confirm the relationships between the variables, suggested by the literature and previous studies. In the results, no statistically significant differences were identified between the sociodemographic profile and the sub-dimensions of individualization. Nurses overall have a good perception of the importance of individualized care (4.06 ± 0.46 ICS-A-NURSE), but face difficulties in its implementation during the last shift worked (3.97 ± 0.49 ICS-B-NURSE). The sub-dimensions more integrated into the care environment were clinical situations and decisional control, and the less integrated were the personal lives of patients. This study identifies key aspects of the individualization of nursing care in acute and perioperative settings, as its omission represents a failure to address the multidimensional health needs of patients, families, and caregivers. We suggest the development of training programs that promote a holistic approach, as well as future studies that consider patient outcomes related to the individualization of nursing care.

Preprint ARTICLE | doi:10.20944/preprints202403.1327.v1

Volatile Constituents of Some Myrtaceous Edible and Medicinal Fruits from the Brazilian Amazon

Adenilson S. Barroso, Lais T. Massing, Chieno Suemitsu, Rosa Helena V. Mourão, Pablo Luis B. Figueiredo, José Guilherme S. Maia

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Edible Brazilian Amazon fruits; volatile concentrates; terpenes and fatty acid derivatives

Online: 22 March 2024 (10:00:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1549.v1

Clinical Outcome of Patients with Escherichia coli Isolated from Catheter Lumens and/or Peripheral Blood Cultures: A Retrospective Analysis

Álvaro Irigoyen-von-Sierakowsi, Marta Díaz-Navarro, Andrés Visedo, María Jesús Pérez-Granda, Pablo Martín-Rabadán, Patricia Munoz, María Guembe

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Escherichia coli; bacteremia; catheter; lock therapy; differential time to positivity; biofilm; outcome.

Online: 23 April 2024 (16:57:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1153.v1

Optimization of the Culture Conditions of Bacterial Strains (Escherichia coli LR0250096.1 and Pseudomonas aeruginosa CP031449.2) to Produce Lignin Peroxidase

Sindiswa Lungile Dube, Foluso Oluwagbemiga Osunsanmi, Albert Oluwafemi Ikhane, Rebamang Anthony Mosa, Andrew Rowland Opoku

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Biodegradation; Lignin peroxidase; kraft lignin; E. coli, P. aeruginosa

Online: 17 November 2023 (12:38:41 CET)

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