With the development of organizational biology and forced from the EU Cosmetic Regulation (EU/1223/2009) with the complete ban of animal testing for cosmetic purposes, 3D skin models have been widely developed due to their similar physiological structure and metabolic function to native human tissue. 3D skin models for in vitro cosmetic evaluation are still promising alternative methods for evaluating phototoxicity, corrosivity and irritation. These models have been used for early screening and later validation of toxicity and efficacy evaluation by many well-known cosmetic companies. 3D skin models have become an important tool in cosmetic evaluation research. This article reviews the development of 3D skin models and their application in cosmetic evaluation. An outlook on the current application and future technological development directions in the cosmetic field is also presented.

The affinity constant, also known as the equilibrium constant, binding constant, equilibrium association constant, or the reciprocal value, the equilibrium dissociation constant (K_d), can be considered as one of the most important characteristics for any antibody-antigen pair. Many methods based on different technologies have been proposed and used to determine this value. However, since a very large number of publications and commercial datasheets do not include this information, significant obstacles in performing such measurements seem to exist. In other cases where such data are reported, the results have often proved to be unreliable. This situation may indicate that most of the technologies available today require a high level of expertise that does not seem to be available in many laboratories. In this paper, we present a simple approach based on standard immunoassay technology that is easy and quick to perform. It relies on the effect that the molar IC₅₀ approaches the K_d value in the case of infinitely small concentrations of the reagent concentrations. A two-dimensional dilution of the reagents leads to an asymptotic convergence to K_d. The approach has some similarity to the well-known checkerboard titration used for the optimization of immunoassays. A well-known antibody against the FLAG peptide, clone M2, was used as a model system and the results were compared with other methods. This approach could be used in any case where a competitive assay is available or can be developed. The determination of an affinity constant should belong to the crucial parameters in any quality control of antibody-related products and assays and should be mandatory in papers using immunochemical protocols.

This article presents a new input device for spastic patients and others with similar symptoms. The sensor consists of a disc used to determine positions and can replace standard joysticks in medical devices such as electric-powered wheelchairs. Using a standard joystick while operating a powered wheelchair can result in dangerous situations when spastic movements occur and extremities cramp making them uncontrollable. To avoid this, a disc was developed that can be controlled with any body part. By shifting weight (x- and y-axis) the disc can tilt in any direction creating a proportional output signal and can also be pushed down in the center (z-axis) to open a menu on a screen for example. When spasms occur it is impossible for users to get stuck on the input device because the disc is flat and can be mounted within a control panel. Body parts coming into contact with the disc would merely slide across the disc without triggering unintentional actions. The sensor presented here is also adaptive and can be adjusted to fit a user’s strength and range of motion. This proposal aims to develop an input device that enables spastic patients to operate sensitive systems safely.

A novel determination method using flow-through cartridge purification and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was developed for the identification and quantification of 30 mycotoxins (aflatoxin B1, B2, G1, G2, sterigmatocysin, T-2 toxin, diacetoxyscirpenol, roquefortine C, methylergonovine, ergocornine, lysergol, enniatin A, enniatin A1, enniatin B, enniatin B1, beauvericin, deoxynivalenol, 3-acetylDeoxynivalenol, 15-acetylDeoxynivalenol, patulin, verruculogen, neosolaniol, gliotoxin, HT-2 toxin, wortmannin, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol) in feedstuffs. Mycotoxins were extracted from sample by 0.1% formic acid aqueous solution/acetonitrile (16/84 v/v), then purified by a Cleanert® MC clean-up column. As a result, the pigments and other matrices were efficient removed from the sample extract solution. The purified eluate was collected, then evaporated and redissolved by 0.1% formic acid aqueous solution/acetonitrile (70:30 v/v) for UPLC-MS/MS detection. A BEH Shield RP18 chromatographic column was employed for separation of 30 mycotoxins in a total of 14 min. Multiple-reaction monitoring (MRM) mode was applied for qualitative and quantitative analysis, and matrix calibration curves obtained with the external-standard method was used for quantitation of target analytes. Under optimized conditions, the linearity range was from 2 to 1000 ng/g, and the limit of quantification of the developed method was from 2 to 50 ng/g. The recoveries of 30 mycotoxins spiked in urine samples were from 72.0% to 118.5%, and the relative standard deviation was below 20%. The method was also well approved by certified reference sample, and applied on the real feedstuff samples testing successfully.

Computer vision is a rapidly developing field that focuses on highly sophisticated picture analysis, manipulation, and comprehension. Its objective is to analyze what is happening in front of a camera and utilize that understanding to control a computer or robotic system or to present users with fresh visuals that are more enlightening or appealing than the original camera images. Computer vision technologies make it feasible for new user interfaces, augmented reality gaming, biometrics, automobile, photography, movie creation, Web search, and many more applications. This essay seeks to explain how computer vision can be utilized to play blackjack successfully.

In the field of railroad safety, effective detection of surface cracks is critical, necessitating reliable, high-speed non-destructive testing (NDT) methods. This study introduces a hybrid Eddy Current Testing (ECT) probe, specifically engineered for railroad inspection, to address the common issue of ’lift-off noise’ due to varying distances between the probe and test material. Unlike traditional ECT methods, this probe integrates transmit and differential receiver coils, aiming to enhance detection sensitivity and minimize lift-off impact. The study optimizes ECT probes employing different driver coils, emphasizing three main objectives: a) quantitatively evaluating each probe using signal- to-noise ratio (SNR) and outlining a real-time data processing algorithm based on SNR methodology; b) exploring the frequency range proximal to the electrical resonance of the receiver coil; c) examining sensitivity variations across varying lift-off distances. The experimental outcomes indicate that the newly designed probe with figure 8-shape driver coil significantly improves sensitivity in detecting surface cracks on railroads. It achieves an impressive SNR exceeding 100 for defects with minimal dimensions of 1 mm in width and depth. Simulation results closely align with experimental findings, validating the investigation of optimal operational frequency and lift-off distance for selected probe performance, determined to be 0.3 MHz and 0.5 mm, respectively. The realization of this project would lead to notable advancements in enhancing railroad safety by improving crack detection efficiency.

To improve carbon efficiency for urban logistics service system composed of a third-party logistics service provider (3PLs) and an e-business enterprise, low-carbon operation game between them was studied. Considering low carbon technology investment cost and sales expansion effect of low carbon level, profit functions for both players were constituted. Based on their different bargaining capabilities, totally 5 types of game scenarios were designed. Through analytical solution, Nash Equilibria under varied scenarios were obtained. By analyzing these equilibria, 4 major propositions were given, in which, some key variables and system performance index were compared. Results show that the best system yields could only be achieved under the fully cooperative situation; limited cooperation only for carbon emission reduction would not benefit the system performance improvement; E-business enterprise-leading game’s performance overtook 3PLs-leading ones.

In this study, 10%PTFE/PEEK blend were modified by potassium titanate whisker (PTW) and chopped glass fiber (GF), respectively. The blends were prepared by three-screw extruder. Through the investigation of thermal stability, tribological properties, mechanical properties and rheological behavior, the effects of reinforcing agents were determined. The results illustrated that the mechanical properties of 10%PTFE/PEEK blend can be dramatically improved by adding reinforcing agent of PTW or GF, and the reinforcing effect of GF was especially obvious. As for tribological properties, 1% addition was the best proportion. The friction coefficient and wear rate of the blend with 1% PTW were 0.283 and 4.97 × 10^-6 mm³/N · m, which decreased by 7.2% and 21% compared with those of the blend without reinforcing agent.

The purpose of this paper is to explore the implementation of Retrieval-Augmented Gen- 14 eration (RAG) technology with open-source Large Language Models (LLMs). For this purpose, a 15 web-based application named PaSSER that integrates RAG with three models - Mistral:7b, 16 Llama2:7b: Orca2:7b, is developed. In its development various technologies are used, including 17 Blockchain for managing and storing assessment results of built-in testing modules. PaSSER em- 18 ploys a set of evaluation metrics for LLMs’ performance - METEOR, ROUGE, BLEU, Perplexity, 19 Cosine similarity, Pearson correlation, and F1 score. The specialized knowledge base is in the smart 20 agriculture domain. As an illustration, the paper presents results and analysis of two tests. First one 21 is assessing the performance of LLMs across different hardware configurations, the other is deter- 22 mining the model that delivers most accurate and contextually relevant responses within the RAG. 23 The paper also discusses the integration of blockchain technology with LLMs to manage and store 24 assessment results within a blockchain environment. The tests revealed that Mistral:7b demon- 25 strates the best results across most of the evaluated metrics. The discussion section comments on 26 technical and hardware considerations that affect the performance of LLMs. Future development 27 will focus in leveraging new larger pre-trained open-source LLMs, exploring different finetuning 28 approaches, and further integration with Blockchain and IPFS.

Under the development mode of deep blending of emerging technologies, major projects are important for enhancing China's comprehensive national strength and independent innovation capability. Based on the social benefits of major projects, and considering the influence of reputation factors, an evolutionary game model composed of a general contractor and subcontractor of major projects is established. This paper studies the influence of various factors including the income distribution coefficient of collaborative innovation, and MATLAB simulation software is analyzed the influence of factors on an evolutionary path. The results show that a reasonable income distribution coefficient can promote the game behavior of both parties to the direction of active collaborative innovation; The reduction of innovation cost and the improvement of spillover technology absorption capacity can make innovation subjects tend to choose active collaborative innovation strategies. In addition, reputation loss above a specific threshold can effectively inhibit free-riding behavior and encourage innovation subjects to choose active collaborative innovation strategies. The research results provide reference for promoting major projects' technology innovation.

In this work, we designed a MEMS device which allows simultaneous direct measurement of mechanical properties during deformation under external stress and characterization of the evolution of microstructure of nanomaterials within a transmission electron microscope. This MEMS device makes it easy to establish the correlation between microstructure and mechanical properties of nanomaterials. The device uses piezoresistive sensors to qualitatively measure the force and displacement of nanomaterials, e.g., in wire and thin plate forms. The device has a theoretical displacement resolution of 0.19 nm and a force resolution of 2.1 μN. The device has a theoretical displacement range limit of 2.74 μm and a load range limit of 27.75 mN.

In this article, we describe a simple procedure for evaluating the sterility of nutrient media using a shear-horizontal surface acoustic wave (SH-SAW) biosensor. We used the SH-SAW biosensor platform to monitor the time-dependent phase responses of the material for the media that binds to a monolayer of 3-mercaptopropanyl-N-hydroxy succinimide on the biosensor surface. Increases in the phase shift less than 50% of the starting phase shift recorded 180 minutes after the sterilization indicate a successful sterility test of the media. Our devices were then applied to sterility testing using a viable Escherichia coli (E. coli) K-12 solution to confirm our hypothesis. Filtered LB Broth was the negative control, and a 24-hour culture of E. coli was the positive control. Before testing, the cell solution was pelleted, the media decanted, and then replaced with a 1x phosphate-buffered saline solution (1x PBS). Since we know that the N-hydroxysuccinimide residues will bind to amine groups, only in cases where microbial species are present will the surface binding increase greater than 50%. We confirmed our hypothesis using SH-SAW biosensors and overnight E. coli cultures. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated based on the response from the E. coli K-12 solution over the linear dynamic range, which appears to be 2x10^4 – 2x10^7 CFU per ml and has a sensitivity of S = 1.655x10^5 o m2 Kg-1. The limit of detection (LOD) for these sensors was calculated to be approximately 2x10^-9 grams (g), which is estimated to be approximately 2,000 Escherichia coli (E. coli) K-12 cells. However, the sensitivity and insertion loss remained approximately unchanged before and after coating the sensor with 3,3'-Dithiobis(succinimidyl) Propionate (DSP).

The effects of rare earth Ce on the microstructure and properties of cast pure copper were investigated through thermodynamic calculations, XRD analysis, mechanical testing, metallographic microscopy, and scanning electron microscopy (SEM). The experimental results demonstrate that the reaction between rare earth Ce and oxygen as well as sulfur in copper exhibits a significantly negative Gibbs free energy value, indicating a strong thermodynamic driving force for deoxidation and desulfurization reactions. Ce is capable of removing trace amounts of O and S from copper. Moreover, the maximum solid solubility of Ce in Cu falls within the range of 0.009% to 0.01%. Furthermore, Ce can refine columnar grains while enlarging equiaxed grains in as-cast copper.Upon addition of rare earth Ce, the tensile strength increased by 8.45%, elongation increased by 12.1%, and microhardness rose from 73.5HV to 81.2HV-an increase of 10.5%. Overall, rare earth Ce has been found to enhance both the microstructure and mechanical properties of cast pure copper.

REDD+ (Reducing Emissions from Deforestation and Forest Degradation) aims to achieve its purpose by working across multiple sectors and involving multi-level actors in reducing deforestation and forest degradation in tropical countries. By contrast, the European Union (EU) Action Plan on Forest Law Enforcement, Governance and Trade (FLEGT) and its Voluntary Partnership Agreements (VPAs) focus on forestry and functions at bilateral state level. The FLEGT Action Plan specifically aims to tackle illegal logging, legalise timber production and trade improve forest governance in countries exporting tropical timber to the EU. Since illegal logging is just one driver of deforestation and forest degeneration, and legalisation of logging does not necessarily reduce deforestation and forest degradation, the two instruments differ in scale and scope. However, by addressing the causes of deforestation and forest degradation and their underlying governance issues, the EU FLEGT and REDD+ share many functional linkages at higher levels of forest policy and forest governance. The contribution and participation of civil society organisations (CSOs) and other actors is imperative to both processes. Our study is based on a survey of key actors (national and international) in REDD+ and FLEGT VPA processes in the Lao People’s Democratic Republic. Our analysis was guided by the theoretical perspectives of the policy arrangement approach and examination of two specific dimensions of this approach, namely resources and rules of the game. This paper argues that participation of CSOs in both processes is crucial as it facilitates and nurtures the very much needed cooperation between other national and international actors. The paper also argues that participation of CSOs feeds valuable information and knowledge into REDD+ and FLEGT VPA processes, thus contributing to increased legitimacy, justice and transparency.

Background and Objectives Neurofibromatosis 1 (NF1 MIM # 162200), also known as von Recklinghausen disease, is among the most common autosomal dominantly transmitted disorders characterized mainly by neurocutaneous manifestations such as café au lait spots, intertriginous freckling, various types of neurofibromas (cutaneous neurofibromas or plexiform tumors), neurological manifestations, and bone abnormalities. It occurs with an incidence of 1:3000–1:4000 cases. Cone-rod dystrophies belong to the large group of retinal dystrophies, a highly heterogeneous group of disorders clinically manifested mainly by damage to cone and rod cells and have a broad spectrum of clinical manifestations. Many genes are involved in their etiology, with more than 100 currently identified, including the CRX gene responsible for the autosomal dominant form of cone-rod dystrophy (ad CORD). Materials and Methods: The authors present an intriguing case of a patient diagnosed with Neurofibromatosis type 1 (NF1) as an infant and ocular involvement (myopia and astigmatism onset at age 15), which yielded a surprising molecular testing result. Results: Molecular DNA analysis identified a rare occurrence of two mutational variants, including a pathogenic variant in the NF1 gene, c.3871-2A, and a likely pathogenic variant in the CRX gene c.119G>A. Conclusions: The authors present this case to highlight the surprises offered by molecular testing in a condition where the diagnosis is usually based on clinical criteria, thus providing patients with competent genetic advice.

The philosophy of testing statistical hypothesis is a natural consequence and functional extension of mathematical analysis of Probability. Along with the concept of recurrence when applied to random sequences and functions, it leads to the analysis of a priori and posterior which implies testing statistical hypothesis. Testing statistical hypothesis also involves algebraic, functional and dimensional considerations, which are found in the works of Laplace. Aspects of mathematical analysis such as universality of solutions, Laws of Large Numbers, Entropy, Information, and various functional dependencies are the main factors explained in the five properties that lead to implication of testing statistical hypothesis. Various interesting examples with modern scientific significance from genetics, astrophysics, and other areas give methodological access to answers of different problems and phenomena which are involved in the logic of testing statistical hypothesis.

This study measured secondary students’ digital literacy using a digital game-based assessment system that was designed and developed based on the Evidence-Centered Game Design (ECGD) approach. A total of 188 secondary students constituted the valid samples in this study. Fine-grained behavioral data generated from students’ gameplay processes were collected and recorded with the assessment system. The Delphi method was used to extract feature variables related to digital literacy from the process data, and the Analytic Hierarchy Process (AHP) method was used to construct the measurement model. The assessment results of the ECGD-based assessment had a high correlation with standardized test scores, which have been shown to be reliable and valid in prior large-scale assessment studies.

This study introduces a novel computational approach for testing conditional independence (BB CI test) within causal Directed Acyclic Graphs (DAGs), leveraging Bayesian non-parametric bootstrap and machine learning techniques. Our method offers an alternative for validating the assumptions underpinning causal DAGs. Through simulation studies and an industrial case analysis, we demonstrate the test procedure in accurately assessing conditional independence, comparing it with the Generalized Covariance Measure (GCM) test. Our findings suggest that the BB CI test is advantageous in scenarios where existing methods may falter due to violations of model assumptions. This research contributes to the causal inference literature by providing a computational tool for researchers and practitioners to validate causal models.

Concrete contributes 8% of all global carbon emissions making the need to find substitute critical for environmental sustainability. Research has indicated the potential for recycled plastics to be used as concrete substitutes. This study extends existing research by investigating the use of polycarbonate (PC) in plastic sand bricks as a mechanical equivalent to concrete. PC has high compressive strength, durability, impact strength, thermal resistivity, clarity, fatigue resistance, and UV resistance. This work provides a method and mold to produce a matrix of sand-plastic sample compositions with dimensions adhering to ASTM D695 standard for compressive properties of rigid-plastic. Compositions of 0% (control), 20%, 30%, 40%, and 50% sand by weight were tested. Samples were tested for compressive strength until yield and stress-strain behaviors plotted. The results for 100% PC demonstrated an average and maximum compressive strength of 71 MPa and 72 MPa, respectively. The 50% PC and 50% sand composition yielded an average and maximum compressive strength of 71 MPa and 73 MPa respectively with an increase in compressive stiffness, and transition to shear failure resembling cement. With a composite density of 1.86 g/cm3 against concrete’s average 2.4 g/cm3, and a compressive strength exceeding commercial concrete demands of 23.3 MPa to 30.2 MPa, this lightweight alternative meets the strength demands of concrete, reduces the need for new construction materials, and provides an additional recycling opportunity for nonbiodegradable waste plastic.

Analyzing BRCA1/2 tumor pathogenic variants (TPVs) in epithelial tubal/ovarian cancers (EOC) has become an essential part of the diagnostic workflow in many centers to guide treatment options and genetic cascade testing. However, there is no standardization of testing procedures, including techniques, gene assays, or sequencers used, and data on the execution of tumor tests remains scarce. Therefore, we evaluated characteristics of BRCA1/2 tumor testing in advanced-stage EOC with real-world national data. Pathology reports of patients diagnosed with EOC in 2019 in the Netherlands were obtained from the Dutch Pathology Registry (PALGA), and data regarding histological subtype and BRCA1/2 tumor tests were extracted. A total of 999 patients with advanced-stage EOC were included. Tumor tests were performed for 502 patients (50.2%) and BRCA1/2 TPVs were detected in 14.7%. Of all tests, 48.6% used hybrid capture techniques and 26.5% used PCR-based techniques. More than half of the tests (55.0%) analyzed other genes in addition to BRCA1/2. Overall, this study highlights the heterogeneity in the execution of BRCA1/2 tumor tests. Despite a lack of evidence of quality differences, we emphasize that adequate reporting and internal and external quality monitors are essential for high-quality implementation and execution of reliable BRCA1/2 tumor testing, which is crucial for identifying all patients with BRCA1/2 TPVs.

Regardless of country and age, the importance of history education is always being emphasized. Although the importance of history education is being emphasized in Korea, there are many difficulties in getting students to understand history properly through school classes alone, and it is also difficult to attract students to participate in classes. The effectiveness of education using games has been proven 20 years ago, and the demand for game-based education is gradually increasing in the current education world, which is becoming more open. In this paper, based on the effects proven through research on the existing game-based education, the improvement of historical thinking ability, experiential history learning, and the problems of game-based education introduced in the ESN report and the discomfort of teachers who participated in the education were improved. A plan was suggested to select and use games suitable for basic education. In this thesis, we selected a history game with a clear historical and periodic background and without distortion of history, and experimented with teaching using games focusing on historical thinking and empirical history learning. The learning achievement of textbook-based education was compared.

Regardless of country and age, the importance of history education is always being emphasized. Although the importance of history education is being emphasized in Korea, there are many difficulties in getting students to understand history properly through school classes alone, and it is also difficult to attract students to participate in classes. The effectiveness of education using games has been proven 20 years ago, and the demand for game-based education is gradually increasing in the current education world, which is becoming more open. In this paper, based on the effects proven through research on the existing game-based education, the improvement of historical thinking ability, experiential history learning, and the problems of game-based education introduced in the ESN report and the discomfort of teachers who participated in the education were improved. A plan was suggested to select and use games suitable for basic education. In this thesis, we selected a history game with a clear historical and periodic background and without distortion of history, and experimented with teaching using games focusing on historical thinking and empirical history learning. The learning achievement of textbook-based education was compared.

The Double U-pipe ground heat exchanger, recognized for its straightforward process, cost-effectiveness, high heat exchange efficiency, and low thermal resistance, stands as the predominant ground heat exchanger type in today's shallow geothermal energy development and utilization. Recent years have seen significant research into the heat transfer influencing factors and heat exchange performance of Double U-pipe ground heat ex-changers via experimental testing methods. Yet, studies integrating numerical simulation with in-situ testing have been less frequent. Drawing on the cylindrical heat source model theory and the outcomes of regional in-situ thermal response tests, this paper develops a Double U-pipe ground heat transfer model by establishing physical, mathematical, and heat transfer geometric models. It assesses the impact of varying inlet temperatures, flow rates, and initial ground temperatures on heat exchange efficiency under heating conditions. The results validate the precision of the double U-pipe ground heat exchanger model based on in-situ testing. They show that enhancing the temperature differential between the inlet and initial temperatures, elevating the initial ground temperature, and moderately increasing the flow rate can boost the system's heat exchange efficiency.

Rivers across the globe experience and respond to changes within the riparian corridor. Disturbance of the riparian corridor can affect warmwater, intermediate, and coldwater streams, which can negatively influence instream physical structure and biological communities. This study focused on assessing the influence of riparian habitat on instream structure within the Whitewater River, a coldwater stream system within an agricultural watershed in southeastern Minnesota, USA. Twenty variables (riparian, n = 9; instream, n = 11) were measured at 57 sites across three forks of the Whitewater. Canonical correlation detected significant associations between riparian and instream variables across the river forks, and indicated that wider riparian buffers, more bank grass and shrubs, longer overhanging vegetation, limited bare soil and more rocks on banks were significantly associated with increased instream cover, high levels of coarse substrates with reduced embeddedness, increased pool habitats, and reduced fine sediments. In contrast, excessive fine sediments, lack of riffle habitat, reduced coarse substrates, and high width to depth ratios indicative of impaired instream habitat were associated with narrow riparian buffers and high percentages of bare soil on banks. Riparian corridors have the capacity to enhance and protect physical instream habitat and overall ecosystem health when managed properly. Wide, grassy riparian corridors with stable banks, overhanging vegetation, and limited shade from trees should protect and/or enhance instream physical habitat, providing the structural diversity favored by aquatic communities.

Defining measures of network efficiency and vulnerability for networks is a pivotal aspect in modern networking paradigms. We approach this problem from a game-theoretical perspective. We will consider networks in which actors have social or economic interests modeled by means of a cooperative game. This allows to define, for each network, a family of efficiency measures and another of vulnerability measures, parameterized by the game. The proposed measures use the within groups and the between groups Myerson values. These two values respectively measure which part of the classical allocation of Myerson corresponds to the productivity of players and which part to the intermediation costs. Also, which part of total centrality in social networks is due to communication or to beteewnness. In our proposal, the efficiency of a network is the proportion of the total productivity (or centrality) that players can retain using the network topology. The intermediation costs (and the betweenness centrality) can be viewed as a weakness with a negative impact. Then, we propose to calculate vulnerability as the proportion expent by players in intermediation payments. We explore the properties of these measures and we particularize them to several structures and particular games, also analyzing their asymptotic behaviour.

This article analyzes the design of MasterMind, an escape room that served as a means of professional development in the use and implementation of online educational tools in academic teaching. Escape rooms have inspired educators all over the world to adapt the popular entertainment activity for education. The time-constrained and problem-based games require active and collaborative participants, which makes an escape room an interesting setting for educators. As there are differences in the settings and goals of educational and recreational escape rooms, there is a need for description of the design process, taking into account game design and educational aspects. MasterMind was developed by a multidisciplinary team of educators, educational researchers and game researchers. The design analysis of MasterMind focuses on three related challenges that have informed the design process: 1) the participants' transition from the real world to the game world; 2) the alignment of game design aspects and educational aspects in the game world; and 3) the transfer from experiences and knowledge obtained within the game world back into the real world. The description and analysis is guided by frameworks on persuasive games and the alignment of game goals and learning goals. The analysis gives insights in how to balance game and educational aspects in the design, in order for players to reach both persuasive and learning goals. We recommend an integrated approach of the different design challenges. Therefore, we propose a design model combining and aligning the used frameworks, leading to an integrated approach in tackling design challenges in persuasive, serious games.

Rapid SARS-CoV-2 self-tests have the potential to expand access to COVID-19 testing and improve community-level case detection, particularly in resource-constrained countries such as Kenya. However, prior to their introduction, their acceptability must be assessed. This qualitative study explored key decision-takers’ values towards SARS-CoV-2 self-testing in Kenya. Healthcare workers, representatives of civil society, and potential implementors from Mombasa and Taita-Taveta were selected as decision-takers. Semi-structured interviews and focus group discussions were used to collect data on their values towards self-testing. A thematic analysis approach was applied. Most informants considered that the Kenyan public is equipped to accept and use self-testing safely as an approach to help to reduce workload at public healthcare facilities, and know one’s COVID-19 status in a private manner. The informants emphasized the need to provide counselling to end-users, to support those needing to self-isolate, and to engage different civil society stakeholders in information provision on self-testing. Fear of stigma and of forced isolation were noted as potential deterrents to self-testing uptake for some individuals. In conclusion, there is high acceptability of self-testing in Kenya among decision-takers. However, enhanced education, counselling, and addressing deterrents to testing would be helpful to ensure effective use of SARS-CoV-2 self-testing in Kenya.

This paper presents an analysis of the sensitivity of controlling an autonomous surface object among a group of encountered objects to the inaccuracy of state control process data. For this purpose, a safe and optimal model of the control process was defined. An algorithm for determining the optimal and safe trajectory of an object, based on the multi-object game model, was developed; for comparative analysis, an algorithm for calculating only the optimal trajectory, not taking into account the maneuverability of other objects, was additionally developed. First, simulation-based studies of the algorithms enabled a comparative analysis of a number of different acceptable strategies for optimally shifting the trajectory of maneuvering objects from a single initial direction. Thereafter, the main goal of this paper was implemented: an analysis of the sensitivity characteristics of safe control, assessed with the risk of collision, both on the inaccuracy of navigation data and on the number of possible control strategies. Finally, final conclusions and a plan for further research on the subject of the paper were formulated.

Creative thinking is increasingly recognised as an essential ability that should be part of school curricula. Given the move towards online learning and assessment, we investigate whether mathematical creativity can be assessed at-scale in the Numbers game, an arithmetic game in Math Garden, a popular online math practice platform. In the Numbers game, a generalisation of the 24 Game, children are asked to figure out how to compute a target number using basic arithmetic operations and a given set of numbers. We argue that creative thinking is required when the search space is complex, and propose that the base-pattern, i.e., the sequence of the operations needed to solve a Numbers game item, indicates search space complexity. We then demonstrate that items with disordered base-patterns are more likely to require mathematical creativity to figure out. Specifically, our analysis shows that for items with only one solution sequence, those with disordered base-patterns are more difficult and take longer to solve compared to items with ordered base-patterns. For items where multiple solution sequences are possible, nine times out of ten children choose ordered over disordered base-patterns. We conclude that the Numbers game has potential for assessing mathematical creativity at-scale.

Nowadays, unmanned vehicles are widely used in various fields. However, the safety of unmanned vehicles is directly determined by the autopilot systems, and sensor faults are a very critical factor that affects the normal operation of the autopilot systems. To efficiently and comprehensively test unmanned systems for sensor faults, this paper proposes a chip-level testing platform for unmanned vehicle autopilot systems based on FPGA hardware-in-the-loop simulation. Unlike existing testing platforms, the introduction of FPGA technology allows us to achieve nanosecond real-time simulation frequency, thus realizing chip-level simulation of sensors and black-box automated testing. Firstly, we propose a set of general platform and modeling methods, and the paper presents a detailed application of the four most common necessary sensors in autopilot systems as examples. Secondly, we propose for the first time a chip-level fault testing method for unmanned systems, and build a test platform with a quadrotor vehicle, the PX4 software platform, and the Pixhawk4 hardware platform as examples. Finally, the experimental results verify that the testing platform proposed in this paper can adapt to various autopilot systems and has high simulation test reliability.

Background: 3D virtual reality (VR) Motionless Imagery Exercise through Avatar (MIEA) was developed to improve elderly’s psychological (cognitive and emotional) and physical health. This study observed the impact of this program on the cognitive functions and emotional well-being and the electrophysiological changes in older adults. Methods: This study was conducted using a randomized controlled trial design. The participants included 38 adults, 65 to 84 years of age, who were physically mobile and neurologically healthy. Among them, 19 participants were assigned to the experimental group, who underwent exercises using elderly avatars in a VR virtual exercise program. The other 19 participants were assigned to the control group who underwent the VR program. Both groups engaged in the VR intervention for 20 minutes three times a week, over six weeks. Results: The cognitive functions, including attention restraint, working memory, and phonemic fluency, of the experimental group showed significant improvements compared to the control group. Regarding emotional well-being, the experimental group showed greater self-efficacy. The electrophysiological changes revealed similar patterns to exercise-induced reactions in electromyography of the experimental group after intervention. Conclusions: These findings confirm that participation in 3D VR MIEA enhances cognitive functions and physical self-efficacy among the elderly. Therefore, 3D VR MIEA can be utilized in the rehabilitation therapy of patients or elderly with limited physical activity.

An influenza A virus (H3N2) with an envelope with the same structure as COVID-19 was used for antiviral testing of material surfaces, in comparison with some results of feline calicivirus. The evaluation method used was the film adhesion method, in which the virus solution is placed on the material and adhered to it using a film. The evaluation method was based on the film adhesion method, in which the virus solution is placed on the material and adhered with a film, and the plaque method, in which the cell virus is allowed to infect the material and form plaques, but the appropriate virus concentration must be adjusted to observe the plaques. When not under that restriction, the plaques are often incomplete; this is observed. For this reason, this study applied an area measurement method that can reflect viral activity even when plaques are not obtained. This study revealed that different types of metallic materials have different antiviral properties. Specifically, the antiviral activity of iron, carbon steel, and nickel was relatively high, while that of stainless steel and titanium was relatively low. The order of antiviral activity was as follows: Fe, SS400, Ni, SUS316L, SUS304, and Ti.

Starting from a very simple economic scenario, we build on it a game and then we introduce a general strategy able to reduce a regression problem to an equivalent binary classification problem. This reduction scheme (that we call adaptive reduction or also dynamic reduction) can be also used to derive a new boosting algorithm for regression problems named bOOst^d. The bOOst^d algorithm is very simple to implement, and it can use any learning algorithm with no priori assumptions. We present a conjecture for bOOst^d performances, which ensures a little error on training set. More important we can also provide a very good theoretical upper bound for the generalization error. We give a set of preliminary experimental results that seems to confirm our conjecture for bOOst^d performances on training set and the theoretical assumptions for the generalization error. We also provide a possible justification of why boosting often does not overfit. Finally, we leave some open problems and argue that in the future an adaptive single boosting (with an unique code) algorithm for binary, multi class and regression problems can be derived.

This paper investigates the problem of the optimal arrangement for both UAVs’ caching contents and service locations in UAV-assisted networks based on the context awareness, which considers the influence between users and environment. In the existing work, users within the coverage of UAVs are considered to be served perfectly, which ignores the communication probability caused by line-of-sight (LOS) and non- line-of-sight (NLOS) links. However, the links are related to UAV deployment. Moreover, the transmission overhead should be taken into account. To balance the tradeoff between these two factors, we design the ratio of users’ probability and transmission overhead as the performance measure mechanism to evaluate the performance of UAV-assisted networks. Then, we formulate the objective for maximizing the performance of UAV-assisted networks as a UAV-assisted caching game. It is proved that the game is an exact potential game with the performance of UAV-assisted networks serving as the potential function. Next, we propose the log-linear caching algorithm (LCA) to achieve the Nash equilibrium (NE). Finally, related simulation results reflect the great performance of the proposed algorithm.

In bacteria, enzymes usually comprise of enzymatic domains only. In eukaryotes/mammals, and here particularly in context of signal transduction, proteins with enzymatic functions contain additional modules promoting interactions with cooperating signaling proteins. Often such protein-protein interaction domains, such as SH2- and SH3-domains, help to localize the enzymatic function (e.g. kinase, phosphatase) to another protein, cellular compartment, or membrane nanodomain. In such cases, the interaction domain(s) serve(s) the enzymatic function. However, there are molecular situations in which an interaction domain fulfills its task completely independent of the protein´s enzymatic function and in this review, we call such function the adapter function of an enzyme. Though many stories can be told, we will concentrate on several proteins playing crucial roles in cells of the immune system, such as BTK, PI3K, and SHIP1, as well as in cancer cells, such as proteins of the RAS/ERK MAPK pathway. We will also discuss how these adaptor functions of enzymes determine or even undermine the efficacy of targeted therapy compounds, such as ATP-competitive kinase inhibitors, thereby highlighting the need to develop pharmacological approaches, such as PROTACs, that eliminate the entire protein. We also review how genetic kock-out and knock-in approaches can be leveraged to identify adaptor functions of signaling proteins.

Reinforcement learning is used for air combat problems in recent years, and the idea of curriculum learning is often used for reinforcement learning, but traditional curriculum learning suffers from the problem of plasticity loss in neural networks. Plasticity loss is the difficulty of learning new knowledge after the network has converged. To this end, we propose a motivational curriculum learning distributed proximal policy optimization (MCLDPPO) algorithm, through which agents trained can significantly outperform the predictive game tree and mainstream reinforcement learning methods. The motivational curriculum learning is to help the agent gradually improve their combat ability by observing the agent's unsatisfactory performance and providing corresponding rewards as a guide. Additionally, a complete tactical maneuver is encapsulated based on existing air combat knowledge, and through the flexible use of these maneuvers, some tactics beyond human knowledge can be realized. In addition, we designed an interruption mechanism for the agent to increase the frequency of decision-making when the agent faces an emergency. When the number of threats the agent receives changes, the current action will be interrupted to reacquire observations and make decisions again. Using the interruption mechanism can significantly improve the ability of the agent. To simulate actual air combat better, we built an air combat environment based on digital twin technology to create an air combat engine core such as missiles and radars. Based on this environment, we develop a distributed intelligent training system that employs Redis as the cache and obtains the data generated in the distributed environment through a multi-threaded TCP/IP protocol, which significantly increases the training data throughput and helps the agent accelerates convergence. The experimental results demonstrate that the agent can fully exploit the situational information to make reasonable decisions and affords air combat tactical adaption, verifying the effectiveness of the algorithmic framework proposed in this paper.

The basic challenges of this work are twofold: demonstrating the dependence between the functional and topological qualities of partition networks and finding the simplest—with respect to algorithmic complexity—network elements. The study of these problems is based on finding the solution to an appropriate antagonistic vertex game. The results of the numerical simulations of antagonistic partition games demonstrate that the winner’s graphs are “almost always” dense and hyperenergetic compared to the loser’s graphs. These observations reveal that successful evolutionary mechanisms can be realized, in principle, by the simplest objects (such as viruses).

In this paper, we investigate some kind of Dynkin game under $g$-expectation induced by backward stochastic differential equation (shortly for BSDE). We define the lower and upper value functions $\underline{V}_t=ess\sup\limits_{\tau\in{\mathcal{T}_t}} ess\inf\limits_{\sigma\in{\mathcal{T}_t}}\mathcal{E}^g_t[R(\tau,\sigma)]$ and $\overline{V}_t=ess\inf\limits_{\sigma\in{\mathcal{T}_t}}ess\sup\limits_{\tau\in{\mathcal{T}_t}}\mathcal{E}^g_t[R(\tau,\sigma)]$, respectively. Under some regular assumptions, a pair of saddle point is obtained and the value function of Dynkin game $V(t)=\underline{V}_t=\overline{V}_t$ follows. Furthermore, the constrained case of Dynkin game is also considered.

This paper formulates the classic Monty Hall problem as a Bayesian game. Allowing Monty a small amount of freedom in his decisions facilitates a variety of solutions. The solution concept used is the Bayes Nash Equilibrium (BNE), and the set of BNE relies on Monty's motives and incentives. We endow Monty and the contestant with common prior probabilities (p) about the motives of Monty, and show that under certain conditions on p, the unique equilibrium is one where the contestant is indifferent between switching and not switching. This coincides and agrees with the typical responses and explanations by experimental subjects. Finally, we show that our formulation can explain the experimental results in Page (1998) [12]; that more people gradually choose switch as the number of doors in the problem increases.

In the construction sector, the integration of biobased materials has emerged as a pivotal aspect of EU policies, aimed at promoting sustainable practices throughout the value chain to mitigate resource consumption and carbon emissions. This paper explores the development and validation activities of a bio-based façade system within the Basajaun H2020 project, focusing on enhancing the utilization of biobased components within building envelopes to replace conventional solutions with eco-friendly alternatives. This paper reports the methodologies employed to detect requirements and outline the testing protocols undertaken to validate the façade system design devised within the project focusing on the original façade components as the bio composite profile. Vision and opaque façade modules are prototyped and tested following curtain wall standards standard for performance and acoustic assessments, to showcase the efficacy of the developed solution. The conducted tests demonstrate the feasibility of integrating bio-based components into building envelopes, aligning with project expectations, and prevailing standards for curtain wall façade solutions. Notably, the designed façade system meets technical conditions and project objectives. Nevertheless, the research underscores the need for further refinements to facilitate solution industrialization and explore broader market applicability. Future efforts will focus on addressing these improvements and assessing the scalability of the solution.

Promoting close and sustainable cooperation between schools, enterprises, and government has become an important concern in many countries. Based on evolutionary game theory, this paper constructs a tripartite evolutionary game model of schools, enterprises, and government in order to analyze the stability of strategies of the different players. The results show that the main factor that influences the stability of the strategies of schools and enterprises is the reward of positive cooperation from sources other than the government, and the main factor that influences the stability of the strategy of the government is the benefit of positive cooperation strategy under the scenario where schools cooperate with enterprises. Therefore, the government should focus on implementing more effective policies, such as increasing the incentives and penalties, improving the mechanism for managing conflicts, ensuring the fairness of benefits distribution, and clarifying the responsibilities of different departments; schools should focus on providing more practical curricula and programs for students, training high-quality teachers, and perfecting talent cultivation to meet the needs of enterprises; and enterprises should focus on providing job experiences for students and transforming the results of schools’ teaching and theoretical research into practical productivity.

Existing implementations of game design patterns have largely been confined to theoretical or research settings. Weaknesses in these implementations have prevented game design patterns from being properly evaluated as an educational and practical development tool. This paper examines these weaknesses, describes a method of developing and applying patterns that overcome the weaknesses, and evaluates use of the method for game design education and practice. Weaknesses in existing pattern implementations are: omission of design problems, presumption of functional completeness at the level of pattern languages, narrow topical focus, and lack of a concise, repeatable method for pattern production. Several features of the proposed method were specifically built to address these weaknesses, namely the pattern template, the process for connecting patterns into a language and assessing the language’s scope, a rubric for assessing pattern confidence and interconnectivity confidence, and pattern-building exercises. This method was applied in a classroom setting. Results, as assessed by the evaluation of student work, suggest that creating patterns/pattern languages is an effective pedagogical approach. De-signs produced using designer-created patterns closely align with existing design theory and are clearly understood by students. The above results may indicate that the path to gaining wider acceptance of pattern theory as a design framework within game design is not to produce a universal pattern language but to facilitate the creation of case-specific languages, by students and professional designers, that use a shared ontology and thus can be combined easily to solve the diverse sets of problems faced by these groups.

The objective was to model the teams’ style of play (SoP) in elite football and relate them to the match result. For this, the twenty Spanish first division teams in the 2016-17 season were analysed, using nine interaction performance indicators (IRi). A principal component (PC) analysis was applied. From two PCs four SoPs were established: deep or high-pressure defending, and elaborate or direct attack. The SoPs were distributed according to average performance obtained throughout the championship. The connection between the preferred SoP and the final result was estimated. Teams with elaborate offensive styles and teams defensively minded got better results. In addition, most of the teams showed variability in their SoP. The applications of the study are: 1) the IRi have served to identify SoP and can be used as a reference to optimize team performance; 2) teams should have a varied SoP repertoire, as well as being prepared to deal with different SoPs; 3) particular player profiles should be connected with the desired SoP when creating the squad. 4) clubs should develop a varied range of SoPs at their academies.

This article discusses the use of game design as a method for interdisciplinary project-based teaching in secondary school education to convey informatics and society topics. There is a lot of knowledge about learning games but little background on project-based teaching using game design as a method. We present the results of an analysis of student-created games and an evaluation of a student-authored database on learning contents found in commercial off-the-shelf games. We further contextualise these findings using a group discussion with teachers. Results underline the effectiveness of project-based teaching to raise awareness for informatics and society topics. We further outline informatics and society topics that are particularly interesting to students, genre preferences and potentially engaging game mechanics stemming from our analyses.

Background: The adoption of self-care behaviors among patients with congestive heart failure (CHF) is essential for the management of their health condition. However, there is a lack of tools for estimating self-care in CHF patients. We aim to develop and validate the Hippocratic heart failure self-care scale (HHFSCS). Methods: the scale includes 22 items which are reviewed by a committee of experts. Individuals indicate the frequency at which they follow each self-behavior on a five-point Likert scale. Adult patients with CHF (n=250) from a General Hospital, in Athens, were participated in the study from June 2020 to March 2021. Reliability coefficients and Explanatory Factor Analysis (EFA), using a Varimax rotation and the Principal Components Method were used to assess the psychometric measurements. Results: The Cronbach’s alpha coefficient of the HHFSCS was 0.807. The exploratory factor analysis identified two domains that accounted for 88.44% of the variance of the scale items; however, each sub-scale could not be used as an independent scale. Finally, the test-retest showed a significant strong correlation (r=0.973, p<0.001). Conclusions: The HHFSCS is a reliable and valid tool for assessing self-behaviors in CHF patients. Health professionals can use it in their clinical practice to improve the management of patient’s health conditions.

In response to escalating global demand for poultry, the industry grapples with an array of intricate challenges, from enhancing productivity to improving animal welfare and attenuating environmental impacts. This comprehensive review explores the transformative potential of digital phenotyping, an emergent technological innovation at the cusp of dramatically reshaping broiler production. The central aim of this study is to critically examine digital phenotyping as a pivotal solution to these multidimensional industry conundrums. Our investigation spotlights the profound implications of 'digital twins' in the burgeoning field of broiler genomics, where the production of exact digital counterparts of physical entities accelerates genomics research and its practical applications. Further, this review probes into the ongoing advancements in the research and development of a context-sensitive, multimodal digital phenotyping platform, custom-built to monitor broiler health. I critically evaluate this platform's potential in revolutionizing health monitoring, fortifying the resilience of broiler production, and fostering a harmonious balance between productivity and sustainability. Subsequently, the paper provides a rigorous assessment of the unique challenges that may surface during the integration of digital phenotyping within the industry. These span from technical and economic impediments to ethical deliberations, thus offering a comprehensive perspective. The paper concludes by highlighting the game-changing potential of digital phenotyping in the broiler industry and identifying potential future directions for the field, underlining the significance of continued research and development in unlocking digital phenotyping's full potential. In doing so, it charts a course towards a more robust, sustainable, and productive broiler industry. The insights garnered from this study hold substantial value for a broad spectrum of stakeholders in the broiler industry, setting the stage for an imminent technological evolution in poultry production.

Cooperative localization under complex urban environments has become a solution to replace the Global Navigation Satellite System (GNSS) positioning. Due to utilizing distance measured and information exchanged between vehicles, cooperative localization results in high computational complexity and heavy communication overhead. This paper proposes a cooperative localization method based on evolutionary coalitional game theory, which implements vehicles' location estimation with less communication cost. We select the neighboring vehicles to form a coalition based on the node's square position error bound and communication cost. The location is obtained via exchanging information between vehicles. It is evident from the simulations and results that the proposed method holds low communication overhead while maintaining localization accuracy.

As sonification is supposed to communicate information to users, experimental evaluation of the subjective appropriateness and effectiveness of the sonification design is often desired and sometimes indispensable. Experiments in the laboratory are typically restricted to short-term usage by a small sample size under unnatural conditions. We introduce the multi-platform CURAT Sonification Game that allows us to evaluate our sonification design by a large population during long-term usage. Gamification is used to motivate users to interact with the sonification regularly and conscientiously over a long period of time. In this paper we present the sonification game and some initial analyses of the gathered data. Furthermore, we hope to reach more volunteers to play the CURAT Sonification Game and help us evaluate and optimize our psychoacoustic sonification design and give us valuable feedback on the game and recommendations for future developments.

We applied evolutionary game theory to extend a resource constrained security game model for confidentiality attacks in an Advanced Metering Infrastructure (AMI), which is a component of IoT-enabled Smart Grids. The AMI is modelled as a tree structure where each node aggregates the information of its children before encrypting it and passing it on to its parent. As a part of the model, we developed a discretization scheme for solving the replicator equations. The aim of this work is to explore the space of possible behaviours of attackers and to develop a framework where the AMI nodes adaptively select the most profitable strategies. Using this model, we simulated the evolution of a population of attackers and defenders on various cases resembling the real life implementation of AMI. We discuss in depth how to enhance security in AMI using evolutionary game theory either by a priori analysis or as a tool to run dynamic and adaptive infrastructure defence.

ChatGPT, a groundbreaking natural language processing technology released just three months ago, has attracted significant attention due to its remarkable capabilities. This AI milestone has urged researchers, industry, decision-makers, and governments to examine this technology, including its implications, threats, and benefits. Despite the short period since its release, several researchers have examined ChatGPT from different perspectives. This paper presents a comprehensive review of ChatGPT, highlighting its technical novelties compared to previous models and analyzing existing research from various perspectives. We followed a rigorous methodology to conduct a critical review of existing research on ChatGPT and developed a taxonomy for the different areas of study. Additionally, we identify future challenges and research trends associated with ChatGPT. Our paper is the first critical review of ChatGPT literature, providing valuable insights for practitioners and policymakers. This paper serves as a reference for researchers seeking to advance research on ChatGPT, including its applications and development.

In recent decades, the Variational AutoEncoder (VAE) model has shown good potential and capabilities in image generation and dimensionality reduction. The combination of VAE and various machine learning frameworks has also worked effectively in different daily life applications, however its possibility and effectiveness in modern game design has seldom been explored nor assessed. The use of its feature extractor for data clustering was minimally discussed in literature neither. This paper first attempts to explore different mathematical properties of the VAE model, in particular, the theoretical framework of the encoding and decoding processes, the possible achievable lower bound and loss functions of different applications; then applies the established VAE model into generating new game levels within two well-known game settings; as well as validating the effectiveness of its data clustering mechanism with the aid of the Modified National Institute of Standards and Technology (MNIST) database. Respective statistical metrics and assessments were also utilized for evaluating the performance of the proposed VAE model in aforementioned case studies. Based on the statistical and spatial results, several potential drawbacks and future enhancement of the established model were outlined, with the aim of maximizing the strengths and advantages of VAE for future game design tasks and relevant industrial missions.

In this research article, the notions of SuperHyperDominating and SuperHyperResolving are defined in the setting of neutrosophic SuperHyperGraphs. Some ideas are introduced on both notions of SuperHyperDominating and SuperHyperResolving, simultaneously and as the same with each other. Some neutrosophic SuperHyperClasses are defined based on the notion, SuperHyperResolving. The terms of duality, totality, perfectness, connectedness, and stable, are added to basic framework and initial notions, SuperHyperDominating and SuperHyperResolving but the concentration is on the “perfectness” to figure out what’s going on when for all targeted SuperHyperVertices, there’s only one SuperHyperVertex in the intended set. There are some instances and some clarifications to make sense about what’s happened and what’s done in the starting definitions. The key point is about the minimum sets. There are some questions and some problems to be taken as some avenues to pursue this study and this research. A basic familiarity with SuperHyperGraph theory and neutrosophic SuperHyperGraph theory are proposed.

Reduced-order electrothermal models play a key role in the design and control of lithium- ion cell stacks, calling for accurate model parameter calibration. This paper presents a complete electrical and thermal experimental characterization procedure for coupled modeling of cylindrical lithium-ion cells in order to implement them in a prototype Formula SAE hybrid racing car. The main 4goal of the tests is to determine cell capacity variations with temperatures and discharge currents, to predict the open circuit voltage of the cell and its entropic component. A simple approach for the characterization of the battery equivalent electrical circuit and a two-steps thermal characterization method are also shown. The investigations are carried out on four commercial 18650 NMC lithium cells. The model demonstrated to predict the battery voltage at a RMS error lower than 20mV and the temperature to a RMS error equal to 0.5 ◦C. The authors hope that this manuscript can contribute to the development of standardized characterization techniques for such cells, while offering experimental data and validated models that can be used by researchers and BMS designers in different applications.

This paper presents the results of laboratory tests of CFRP (carbon fibre-reinforced polymer) laminates, which allowed the development of numerical material models. The obtained data were used in a further stage to perform numerical simulations of four variants of medical tabletops differing, among other features, in the shape of the cross-section. Maximum deflections and effort in the composite material were analyzed. The final step was to perform a laboratory test for one of the tabletop versions, the results of which confirmed the correctness of the numerical calculations. This work is aimed at both researchers and designers involved in the practical application of fibre-reinforced polymer matrix composites.

The world is facing a great technological transformation towards full autonomous vehicles, where optimists predict that by 2030, autonomous vehicles will be sufficiently reliable, affordable and common to displace most human driving. To cope with these trends, reliable perception systems must enable vehicles to hear and see all the surroundings, being light detection and ranging (LiDAR) sensors a key instrument for recreating a 3D visualization of the world in real time. However, perception systems must rely in accurate measurements of the environment. Thus, sensors must be calibrated and benchmarked before being placed on the market or assembled in a car. This article presents an Evaluation and Testing Platform for Automotive LiDAR sensors with the main goal of testing not only commercially available sensors, but also sensor prototypes currently under development in Bosch Automotive Electronics division. The testing system can benchmark any LiDAR sensor under different conditions, recreating the expected driving environment to which such devices are normally subjected. To characterize and validate the sensor under test, the platform evaluates several parameters such as the field of view (FoV), angular resolution, sensor’s range, etc. This project results from a partnership between the University of Minho and Bosch Car Multimedia Portugal, S.A.

The need to develop novel lightweight materials and their manufacturing processes is sets out to meet the new aerospace, automotive and construction requirements. Within this context, this research work is proposed to develop a novel thermoplastic composite material with high mechanical properties. These composites will be based on thermoplastic matrixes made from polyamide and 35% short glass fiber filled-polyamide reinforced with different types of fabrics. As reinforcement, glass fiber fabrics will be used as the base. They will be treated with different processes, both chemical and physical, to promote adherence to the matrix. Textile overmoulding technology was selected for manufacturing these composites. This technology was primarily developed to manufacture aesthetic lined components and has achieved a great implantation. Once these new composites are manufactured, they will be submitted to different tests to evaluate their behavior regarding adhesion, impact strength and stiffness. It is expected an improvement on stiffness and impact absorption.

The manual muscle test (MMT) is a flexible diagnostic tool, which is used in many disciplines, applied in several ways. The main problem is the subjectivity of the test. The MMT in the version of a “break test” depends on the tester’s force rise and the patient’s ability to resist the applied force. As a first step, the investigation of the reproducibility of the testers’ force profiles is required for valid application. The study examined the force profiles of n=29 testers (n=9 experiences (Exp), n=8 little experienced (LitExp), n =12 beginners (Beg)). The testers performed 10 MMTs according to the test of hip flexors, but against a fixed leg to exclude the patient’s reaction. A handheld device recorded the temporal course of the applied force. The results show significant differences between Exp and Beg concerning the starting force (padj=0.029), the ratio of starting to maximum force (padj=0.005) and the normalized mean Euclidean distances between the 10 trials (padj=0.015). The slope is significantly higher in Exp vs. LitExp (p=0.006) and Beg (p=0.005). The results also indicate that experienced testers show inter-tester differences and partly even a low intra-tester reproducibility. That highlights the necessity of an objective MMT-assessment. Furthermore, an agreement on a standardized force profile is required – a suggestion is given.

Mycobacteria can be one of the main contaminants of biological products, and their presence can have serious consequences on patients’ health. Thus, specific testing is necessitated by regulations such as the European Pharmacopoeia. The current pharmacopeial reference i.e., microbial culture method cannot ensure an exhaustive detection of mycobacteria due to their growth characteristics. Additionally, the method is time-consuming and requires a continuous supply of culture media, which could be further challenging. Thus, to overcome these challenges, pharmaceutical industries need to consider alternative non-microbiological techniques to detect these fastidious, slow-growing contaminating agents. This review provides an overview of alternative methods, which could be applied within a quality control environment of biological products, and underlines advantages and limitations of these methods. Nucleic acid amplification techniques or direct measurement of mycobacteria stands out as the most suitable alternatives for mycobacterial testing in biological products.

Data-Flow and Higher-Order Mutation are white-box testing techniques. To our knowledge, no work has been proposed to compare data flow and higher-order mutation. This paper compares all def-uses data-flow and second-order mutation criteria. This compassion investigates the subsumption relation between these two criteria and evaluates the effectiveness of test data developed for each. To compare the two criteria, a set of test data satisfying each criterion is generated, which is used to explore whether one criterion subsumes the other criterion and assess the effectiveness of the test set that was developed for one methodology in terms of the other. The results showed that the mean mutation coverage ratio of the all du-pairs adequate test cover is 80.9%, and the mean data flow coverage ratio of the 2nd-order mutant adequate test cover is 98.7%. Consequently, 2nd-order mutation “ProbSubsumes” the all du-pairs data flow. The failure detection efficiency of the mutation (98%) is significantly better than the failure detection efficiency of data flow (86%). Consequently, 2nd-order mutation testing is “ProbBetter” than all du-pairs data flow testing. In contrast, the size of the test suite of 2nd-order mutation is more significant than the size of the test suite of all du-pairs.

The importance of frailty in older people is getting constant recognition as an important aspect both in terms of public health, as well in personal level for the appropriate management of an older person health condition. This is reflected by the continuously increasing number of research studies carried out in several settings across different countries. Sometimes this is very solid, but in other cases there is a considerable gap in terms of accurate and well grounded documentation of frailty status. This is the case in Greece, where we are missing clinically validated tools to approach frailty. We are missing frailty screening tools, such as for instance PRISMA 7, the gold standard tool of Fried criteria is somehow problematic since the question referring to physical activity originates from a questionnaire that has not been translated and validated, while Clinical Frailty Scale has been validated for the translation but not for the capacity to detect frailty. Aim of this study is to validate these tools for their accuracy to detect frailty by using a measurable index of frailty, previously proposed for use in clinical studies: the Short Physical Performance Battery (SPPB). 74 male and female participants have been evaluated for their frailty status using different tools. We observed that PRISMA 7 translation detects frailty only when one question is removed at a cut-off of ≥ 2 and indicated a sensitivity of 88.1%, and specificity of 99.9% with a good correlation with SPPB measurements (r = 0.858; p < 0.001). When CFS was validated using SPPB it demonstrated a very good correlation (r = -0.838; p < 0.001 respectively) as it was the case for the modified Fried Criteria (r = -0.725; p < 0.001). All items demonstrated a good correlation between them. We can here propose that we can accurately assess frailty status in the community setting by using a modified version of Fried criteria, Clinical Frailty Scale translation in Greek and we can screen for frailty by using the Greek translation of PRISMA 7 only after removing the item 6 of the questionnaire.

Saliva, as a diagnostic medium, offers a promising alternative to blood by virtue of its non-invasive collection, which enhances patient compliance, especially in paediatric and geriatric populations. In this study, we assessed the utility of saliva as a non-invasive medium for measuring V. cholerae-specific serum antibodies in naturally infected individuals. We tested paired serum and saliva samples obtained from a total of 63 cholera patients enrolled in a cohort study. Vibriocidal antibodies assay (IgM/IgG) as markers for accurate determination was used to determine cholera specific antibody levels. Using receiver operating characteristics (ROC) curve, we found that the best cut-off that maximizes (sensitivity + specificity) is 10 titers. At this saliva titer, the sensitivity is 76.9% (95%CI: 60.9%, 87.7%) and specificity is 80.0% (95%CI: 56.6%, 92.5%). Using Spearman’s correlation coefficient, we also found evidence of a positive correlation between Vibrio Cholerae saliva and serum antibodies (rho=0.66, p<0.001). In conclusion, saliva-based diagnostic cholera tests has high diagnostic accuracy, and would be advantageous, cheaper, and quicker for early diagnosis of severe cholera outcomes.

Resource pricing in cloud computing has become one of the main challenges for cloud providers. The challenge is determining a fair and appropriate price to satisfy users and resource providers. To determine a fair price, it is necessary to consider the conditions and needs of both parties: provider and user. This research tries to provide a pricing mechanism for cloud computing based on game theory. The proposed mechanism takes into account three factors including fault possibility, interaction between virtual machines, and energy consumption to reach a fair price. In the proposed game, the provider decides on the price of the virtual machine which can be offered to the user on each physical machine and the user decides between the virtual machines offered to run its application. The whole game is implemented as a function of the resource broker component. The proposed mechanism is simulated and evaluated using the CloudSim simulator. Its performance is compared with several previous recent mechanisms. According to the results, the proposed mechanism has been able to determine a more reasonable price for the user and the provider which increases the overall profitability of the cloud system.

Due to the great threat of torpedo against surface warships, an efficient hydroacoustic countermeasure system must output realtime strategies that can accommodate varied antagonizing scenarios. Aiming at the decision-making problem in the anti-torpedo hydroacoustic countermeasure cases, this paper proposes an intelligent adversarial strategy based on the game theory. By discretizing the strategy space of both sides, a matrix game model is established with the payoff characterized by the capture probability of attacking torpedo. An improved simplex algorithm is then developed to get the mixed-strategy Nash equilibrium of the game model, with which preferred avoiding strategies can be obtained to minimize the risk of being captured by the attacking torpedo. Based on the probabilistic view of preferred adversarial strategy, the launching strategy of floating acoustic decoy can be achieved, and the evasion trajectory of warship can be confirmed simultaneously. Numerical simulation demonstrates that the proposed method can generate realtime intelligent antagonizing strategy, and consequently, can improve the survival rate of surface warships in the of anti-torpedo countermeasure scenarios.

In this paper we present an application of the dynamic tracking games framework to a monetary union. We use a small stylized nonlinear three-country macroeconomic model of a monetary union to analyse the interactions between fiscal (governments) and monetary (common central bank) policy makers, assuming different objective functions of these decision makers. Using the OPTGAME algorithm we calculate solutions for several games: a noncooperative solution where each government and the central bank play against each other (a feedback Nash Equilibrium solution), a fully cooperative solution with all players following a joint course of action (a Pareto optimal solution), and three solutions where various coalitions (subsets of the players) play against coalitions of the other players in a noncooperative way. It turns out that the fully cooperative solution yields the best results, the noncooperative solution fares worst, and the coalition games lie in between, with a broad coalition of the fiscally more responsible countries and the central bank against the less thrifty country coming closest to the Pareto optimum.

The altered nature of archaeological metals means they deteriorate at conditions where metals would be stable. The study of deterioration for such materials is hampered by their complexity, variability and difficulties in measuring deterioration. Placing an object in a sealed container, controlling the RH and pollutant gases and measuring any decrease in oxygen concentration is an accessible method to measure deterioration rate. It has been used for research into suitable environmental conditions to manage deterioration rates of such artefacts including differences in response for artefacts from different excavation sites. Some objects need careful control of RH to low values, this is expensive to maintain and poses risks to other artefacts displayed together. Many objects are actually stable up to quite high RH values and oxygen depletion testing has been used to identify those that can be safely displayed with minimal environmental control. The accelerated corrosion, ‘Oddy’ test is frequently used to sift out unsuitable display materials. The visual assessment is widely recognized to be subjective. The test container has been modified and oxygen depletion appears to give good quantitative measurements of corrosion that correspond with both visual comparison and corrosion quantification for copper, lead and steel, but not for silver.

A number of experimental tests of time orientability are described as well as clear experimental signatures from non time orientability (time reversal). Some tests are well known, while others are based on more recent theoretical work. Surprisingly, the results all suggest that time is not orientable at a microscopic level; even definitive tests are positive. At a microscopic level the direction of time can reverse and a consistent forward time direction cannot be defined. That is the conclusion supported by a range of well-known experiments. The conflict between quantum theory and local realism; electrodynamics with electric charges; and spin half transformation properties of fermions; can all be interpreted as evidence of time reversal. While particle-antiparticle annihilation provides a definitive test. It offers both a new view of space-time and an novel interpretation of quantum theory with the potential to unify classical and quantum theories.

The unprecedented COVID-19 pandemic posed major challenges to local, regional, and global economies and health systems, and fast clinical diagnostic workflows were urgently needed to contain the spread of SARS-CoV-2. Here, we describe the platform and workflow established at the Cornell COVID-19 Testing Laboratory (CCTL) for high-throughput testing of clinical samples from the university and the surrounding community. This workflow enabled efficient and rapid detection, and successful control of SARS-CoV-2 infection on campus and its surrounding communities. Our cost-effective and fully automated workflow enabled testing of over 8,000 pooled samples per day and provided results for over 2 million samples. Automation of time- and effort-intensive sample processing steps such as accessioning and pooling increased laboratory efficiency. Customized software applications were developed to track and store samples, deconvolute positive pools, track and report results and for workflow integration from sample receipt to result reporting. Additionally, quality control dashboards and turn-around time tracking applications were built to monitor assay and laboratory performance. As infectious disease outbreaks pose a constant threat to both human and animal health, the highly effective workflow implemented at CCTL could be modeled to establish regional high-capacity testing hubs for infectious disease preparedness and emergency response.

Spironucleosis is considered one of the most significant enteric infectious diseases in young and growing game birds. Despite its importance diagnosing spironucleosis in game birds remains a continuing problem as no 'gold standard' test exists and there is no consensus for the use of the available methods for the diagnosis of spironucleosis in game birds. In this study we describe veterinarians’ perceptions of the adoption of existing methods for the diagnosis of spironucleosis in game birds and identify implications for the development of diagnostic guidelines. A questionnaire was used to evaluate participants’ familiarity with and use of existing diagnostic methods for spironucleosis, and to assess their perceptions of the adoption of these methods in their geographic regions. We surveyed veterinarians in practices in East Anglia (EA) and Northern Ireland (NI), UK with expertise or involvement in the treatment and/or diagnosis of spironucleosis in game birds. Of the 261 participants surveyed, 15 EA and 13 NI veterinarians (10.7%) responded to the questions on existing diagnostic methods. The study results indicate an overall insufficient knowledge and skills in the diagnosis and treatment of spironucleosis among veterinarians surveyed. These findings highlight the need for improved training of veterinarians in the diagnosis of spironucleosis.

In this paper, we consider a pursuit-evasion game of inertial players, where pursuer’s control is subject to integral constraint, and evader’s control is subject to geometric constraint. In the pursuit problem, the main tool is the strategy of parallel pursuit. Sufficient conditions are obtained for the solvability of pursuit-evasion problems. Also, the main lemma describing the monotonicity of an attainability domain of the evader is proved and an explicit analytical formula for this domain is given. As one of the important results of our paper, the Isaacs Life-line game is solved for a special case.

^{We consider the collective pricing orders in a minimum supply chain that is composed of a monopolistic manufacturer and many retailers that belong to the same chain store firm. The retailers have the freedom to raise or lower the local price. The chain store firm sets up the commercial rules for local retail stores to maximize its total payoff. The monopolistic manufacturer firm controls the total quantity supplied for the market to achieve maximum benefits. We applied the two dimensional Ising model in statistical physics to map the collective distribution of microscopic strategy of local retailers into the macroscopic total payoff of the chain store firm. The local stores choose to raise the price or lower the price based their own mind when the supply in market surpasses the demand. When the supply in market is far less than the demand, the stores synchronously raise prices, even though a local store only have the incomplete information of their nearest neighboring supermarket. We find the critical equation for the balance point between the action of supplier and the action of chain store management based on game theory and statistical physics. The critical equation can identify the Nash equilibrium point of the non-cooperative game between the manufacturer and the chain-store seller, and reveal different levels of collective operations. This statistical physics method also holds for more complicate supply chains and economic systems.}

Video gamers can play to negate the psychological impact of stress (Pine et al., 2020), which may become problematic when users over-rely on the stress-relief potential of gaming. This study used a repeated-measures experimental design to investigate the relationships between stress, video gaming, and problematic video gaming behaviours in a convenience sample of 40 students at a UK university. Results indicated that positive affect increased and negative affect decreased, whilst a biological stress measure (Instantaneous Pulse Rate) also decreased after a short video gaming session (t(36) = 4.82, p < .001, d = 0.79). Results also suggest that video gaming can act as a short-term buffer against the physiological impact of stress. Further research should focus on testing individuals who have been tested for Gaming Disorder as opposed to a general population. Research could also utilise variations of the methodological framework used in this study to examine the intensity of a stress-relief effect under different social situations. The study’s findings in relation to published work are also discussed.

The rapid integration of Information and Communication Technology (ICT) is transforming the traditional electrical grid into a {\em Smart Grid}. Smart grids enable two-way communication and improved monitoring and control between utilities and customers. However, due to its heterogeneous nature, public exposure, and weak security at low-powered devices, the Smart Grid has vulnerabilities to various malicious threats, adversaries, and cyber attacks, which may affect cost and service availability. Additionally, when the systems' confidentiality, integrity, or availability are compromised, the resulting fallout can threaten national security and have cascading effects on human lives. Given the extreme consequences of an attack, smart-grid technology must be thoroughly tested for correct operation and security {\em before} it is deployed. As a result, vulnerability testing of smart grids, not only for correctness but for security purposes, has been the subject of numerous studies by academics, government agencies, and private companies. This paper reviews the vulnerabilities associated with the smart grid and spotlights simulation as the vulnerability testing methodology conducted in recent pertinent research works. It also presents various security aspects of the smart grid, including grid applications, system and network infrastructure and components, cyber threats and attacks, simulation, and different mitigation techniques. Finally, we analyze the gaps in the current research works, focusing on simulation. We briefly present a real-time simulation testbed that mimics customer behaviour and integrates hardware in the loop to apply attack methods, analyze vulnerabilities and risk mitigation associated with the smart grid system, and propose future work to improve the current framework.

In the paper, a study of the performance of unmanned ground vehicle (UGV), establishes numerical simulation model of the chassis system by three-dimensional software and finite element analysis software. Based on the numerical modelling and analysis, the work focuses on study of the strength and stiffness of the vehicle's chassis and researched the distribution of stress and deformation under the extremal condition. The paper also presents an autopilot design with new cascade control system for an autonomous motion of an unmanned ground vehicle based on Proportional-Integral-Derivative (PID) and Feed-Forward (FF) control. The PID-FF controller is a part of dedicated for presented UGV used in a hybrid control system for precise control and stabilization, necessary to increase the vehicle motion stability and maneuvers precision. The hybrid PID-FF control system proposed for the ground vehicle model gives a satisfactory quality of control, while maintaining the simplicity of the control system. Presented tests performed in area of mechanical design and control analysis gives good results and proves usefulness of designed unmanned device.

In this study, a novel porous geopolymer mortar (GP) was produced and tested experimentally. Industrial waste materials/by-products were used as constituents of the GP, along with dune sand. One sample was produced as a control sample for benchmarking. For the rest of the samples, 15%, 30%, and 45% by volume, the solid constituents were replaced with expanded polystyrene foam (EPS) beads. These mortar samples were heat cured to depolymerize the EPS to cause porosity inside the samples. Indoor experiments were conducted to evaluate the response of produced porous GP to high heat flux. The porous samples were able to reduce heat transmission across the opposite surfaces. Induced porosity resulted in a decrement in compressive strength from 77.2 MPa for the control sample to 15.8 MPa for 45% porous sample. However, the limit lies within the standards for partitioning walls in buildings and pavements in urban areas to absorb rainwater.

Reliable methods to confirm the diagnosis of COVID-19 are essential to the successful management and containment of the virus. Current diagnostic options are limited in type, supply, and reliability. This article explores the controversial unreliability of existing diagnostic methods and maintains that more reliable diagnostic methods, combinations, and sequencing are necessary to effectively assist in reducing the occurrence of discharge of the patient on false negative test results. This reduction would in effect reduce transmission of the disease.

Intelligent transportation systems (ITS) are at the forefront of advancements in transportation, offering enhanced efficiency, safety, and environmental friendliness. To enable ITS, autonomous systems play a pivotal role, leveraging breakthroughs in autonomous driving, data-driven modeling, and multiagent control strategies to establish sustainable and coordinated traffic management. The integration of connected and automated vehicles has garnered significant attention as a potential solution for alleviating traffic congestion and improving fuel economy, achieved through global route optimization and cooperative driving. This study focuses on a predictive control perspective to address the cooperative cruise control problem. Online decision-making is employed during the driving process, utilizing information gathered from the network. By employing bargaining games to establish an operating agreement among vehicles, we formalize a synchronization approach based on predictive control theory. Ultimately, these findings are put to the test in an emulation environment within a hardware-in-the-loop system.

A single paragraph of about 200 words maximum. For research articles, abstracts should give a pertinent overview of the work. We strongly encourage authors to use the following style of structured abstracts, but without headings: (1) Background: Place the question addressed in a broad context and highlight the purpose of the study; (2) Methods: briefly describe the main methods or treatments applied; (3) Results: summarize the article's main findings; (4) Conclu-sions: indicate the main conclusions or interpretations. The abstract should be an objective rep-resentation of the article and it must not contain results that are not presented and substantiated in the main text and should not exaggerate the main conclusions.

As part of the efforts to reduce the environmental impacts caused by the aviation sector, the use of bio-based instead of fossil-based materials has been assessed as a possible mitigation option. Natural Fibre Polymer Composites have proven to have a higher environmental performance in the automotive sector and are emerging as an option for weight reduction in aircraft. This study quantifies, though Life Cycle Assessment, the environmental performance of specific flax-based composite panels intended for aircrafts as interior fitting elements (i.e. partition panels, tray tables, baggage compartments) compared to a glass fibre/epoxy composite with a honeycomb core. Through system expansion, the fate of co-products issued from the production of the flax fibre technical textile used as reinforcement in the biocomposite material were considered in the assessment. Results showed that for an application in the aeronautics sector, the weight of the panels is the upmost critical parameter shaping the overall environmental performance of panels. Focusing on the panel production only, the biocomposite panel showed a higher environmental performance in the categories of climate change and marine eutrophication compared to the conventional panel, and the fire suppressant agent was identified as the main contributor to the environmental impacts of the bio-based panel. Yet these gains were negligible when considering the full life cycle of the panels, due to the higher weight (14%) of the bio-based panels; which is linked to the bio-based panel being at a prototype stage.In order to improve the environmental performance of the biocomposite panel and thus reduce its weight, it was shown relevant to optimize geometry of the panel itself, especially its core, so less resin could be used.

Recently there has been growing interest in the application of game theory (GT) to solve many diverse problems in the field of construction, including i.a.: tender preparation, selection of a contractor for construction works, negotiating terms and conditions of cooperation of the parties to the contract, analysis and modeling of investment risk. In the authors opinion, the use of GT by general contractor (GC) of construction works to indicate the best strategy leading to winning court proceedings in a situation of conflict with investor (IN), so far has not been the subject of research. Taking into account the above, the aim of the presented paper is to indicate the optimal strategy from the GC point of view in the conflict situation with IN. The article presents a list of the most common causes of conflicts between parties of the construction works' contract, defines the background of the problem and the cause of the dispute, and on its basis, the authors generate the theoretical model of the game. Based on the analyzed game model, expected payoffs for players were calculated and the probability border value at which GC should apply the indicated strategy determined. The results of the study show that in the case when the probability of issuing a judgment favorable for GC is at least equal to 69.23%, it is justified to use an aggressive strategy. The analysis also confirms that from the financial perspective, litigation in most cases of conflicts in the area of construction should be the last choice.

UAV cooperative control has been an important issue in UAV-assisted sensor network, thanks to the considerable benefit obtained from cooperative mechanism of UAVs being applied as a flying base station. In coverage scenario, the tradeoff between coverage performance and transmission performance often makes deployment of UAVs fall into a dilemma, since both indexes are related to the distance between UAVs. To address this issue, UAV coverage and data transmission mechanism is analyzed in this paper, then an efficient multi-UAV cooperative deployment model is proposed. The problem is also modeled as a coalition formation game (CFG). The CFG with Pareto order is proved to have a stable partition. Then, an effective approach consisting of coverage deployment and coalition selection is designed, wherein UAVs can decide strategies cooperatively to achieve better coverage performance. Combining analysis of game approach, a coalition selection and position deployment algorithm based on Pareto order (CSPDA-PO) is designed to execute coverage deployment and coalition selection. Finally, simulation results are shown to validate the proposed approach based on efficient multi-UAV cooperative deployment model.

A nonrenewable resource extraction game model is analyzed in a differential game theory framework with random duration. If the c.d.f. of the final time is discontinuous, the related subgames are differentiated based on the position of the initial instant with respect to the jump. We investigate properties of optimal trajectories and of imputation distribution procedures if the game is played cooperatively.

Among the different steps in software development software testing is one of the inevitable steps. Manual testing is considered to be more expensive and time-consuming, and this led to the formation of automated testing tools. Automated software testing tools offer a comprehensive system testing solution that minimizes the risk of any errors that could result in financial losses. One such tool is selenium. Selenium testing is an advantageous method employed by developers and testers to assess their code. Selenium is compatible with numerous widelyused programming languages like C#, Java, Python, and more. This paper focuses on understanding the in and outs of software testing using selenium

A geometric Planck-scale model of quantum electrodynamics is tested against observations. Based on Dirac’s proposal to describe spin 1/2 particles as tethered objects, elementary fermions are conjectured to be fluctuating rational tangles with unobservable tethers. As Battey-Pratt and Racey showed, when such tangles propagate, they obey the free Dirac equation. Classifying rational tangles yields the observed spectrum of elementary fermions. Classifying deformations of tangle cores yields exactly three types of gauge interactions. They exchange three types of elementary gauge bosons and have the symmetry groups U(1), broken SU(2) and SU(3). The possible rational tangles for fermions, Higgs and gauge bosons allow only the observed Feynman diagrams. The complete Lagrangian of the standard model arises, including the Lagrangian of quantum electrodynamics. Over 50 tests of the tangle model are deduced. They include details on the perturbation expansion of the g-factor and the existence of limit values for electric and magnetic fields. Measurements agree with most tests, though this could change in the future. Some tests are genuine predictions that still need to be checked. In particular, the geometry of the process occurring at QED interaction vertices suggests an ab-initio estimate for the fine structure constant. In addition, the average geometric shapes of the elementary particle tangles suggest ab-initio lower and upper limits for the mass values of the electron and the other leptons.

This is an article or technical note which is intended to provides an insight journey of Machine Learning Systems (MLS) testing, its evolution, current paradigm and future work. Machine Learning Models, used in critical applications such as healthcare industry[1], Automobile [2], [3] and Air Traffic control, Share Trading etc., and failure of ML Model can lead to severe consequences in terms of loss of life or property. To remediate this, developers, scientists, and ML community around the world, must build a highly reliable test architecture for critical ML application. At the very foundation layer, any test model must satisfy the core testing attributes such as test properties and its components. This attribute comes from the software engineering [5], [6], but the same cannot be applied in as-is form to the ML testing and we will tell you “why”.

Nowadays, the European Union and the governments of the different countries have focused on the development of the Sustainable Development Goals (SDG) and the 2030 agenda, something that has been translated into education itself. Video Games, Gamification, and Game Based Learning have become different strategies and tools to enhance the learning process and some of the growing approaches used by teachers to develop sustainable education in the classrooms. This research aims to analyze the characteristics to promote sustainability in education using games and technology, specifically its learning benefits for Higher Education. A systematic review of the literature was conducted following the PRISMA methodology. At first, 2025 documents were found which, after the filtering phases, the number of articles has been reduced to nine, which subsequently were analyzed in depth. The results indicated that among the benefits of the use of games mediated by technologies are the following: it favors education for sustainability and it promotes the educational inclusion and the work of various social skills such as collaborative and cooperative work. Also, showed an increase of the number of publications between 2019 and 2023, reflecting the growing interest in the topic. However, there are some research gap in this field.

Mass customization, small batch sizes, high variability of product types and a changing product portfolio during the life cycle of an industrial plant are current trends in the industry. Due to an increasing decoupling of the development of software and hardware components in an industrial context, compatibility problems within industrial control systems arise more and more frequently. In this publication, a strategy concept for compatibility testing is derived and discussed by means of literature review and applied research. This 4-phased strategy concept identifies incompatibilities between software and hardware components in the industrial control environment and enables test engineers to detect problems at an early stage. By automating the compatibility test on an external I-PC, the test can be run both when new software is installed on the industrial controller and when the controller is restarted. Thus, changes to the components are constantly detected and incompatibilities are avoided. Furthermore, early incompatibility detection can ensure that a system remains permanently operational. Based on a discussion, additionally strategies are identified to consolidate the robustness and applicability of the presented concept.

Lipofuscin is indigestible garbage that accumulates in the autophagic vesicles and cytosol of post-mitotic cells with age. Drs. Brunk and Terman postulated that lipofuscin accumulation is the main or at least a major driving factor in aging. They even posited that the evolution of memory is the reason why we get lipofuscin at all, as stable synaptic connections must be maintained over time, meaning that the somas of neurons must also remain in the same locale. In other words, they cannot dilute out their garbage over time through cell division. Mechanistically, their position certainly makes sense given that rendering a large percentage of a post-mitotic cell’s lysosomes useless must almost certainly negatively affect that cell and the surrounding microenvironment. Here, I explore the possibility that the accumulation of lipofuscin may to some extent exacerbate every other kind of age-related damage. I do not think that lipofuscin removal will reverse/prevent all forms of aging, just the major component facing us currently. In this piece, I will review what is known about lipofuscin accumulation from evolutionary and mechanistic standpoints and discuss a method of removing it from single cells in vitro.

Achieving glycemic control and sustaining functional pancreatic β-cell activity remains an unmet medical need in the treatment of type 2 diabetes mellitus (T2DM). Glucokinase activators (GKAs) constitute a class of anti-diabetic drugs designed to regulate blood sugar levels and enhance β-cell function in patients with diabetes. A significant progression in GKA development is underway to address the limitations of earlier generations. Dorzagliatin, a dual-acting GKA, targets both the liver and pancreas and has successfully completed two Phase III trials, demonstrating favorable results in diabetes treatment. The hepato-selective GKA—TTP399— emerges as a strong contender, displaying clinically noteworthy outcomes with minimal adverse effects. This paper seeks to review the current literature, delve into the mechanisms of action of these new-generation GKAs, and assess their efficacy and safety in treating T2DM based on published pre-clinical studies and clinical trials.

The main aim of sustainable development is to ensure an intelligible and long-lasting balance between the economy, society, and the environment. Sustainable tourism could only be successful if the inter-relationships between all three dimensions are accepted. In the limited number of research analyses, the focus of the research is on competition between tourist countries and destinations. This study has used Game theory to analyze the competition applies time-series data in selected neighboring countries measure of a VAR-based spillover index, developed by [1] to investigate the time-varying relationship between tourism and Gross Domestic Product. Each country analyzed Vector Error Correction (VEC) and Granger analysis to explore the causal short and long-term tourism and use a sample that spans from 1997 to 2019. From the main results of Cholesky, the total spillover index is 59.0% between Russia and Mongolia which suggests a moderate interdependence among the four variables. Findings indicate that neither China nor Mongolia have a short-run influence on tourism development. China's inbound tourism is affected in the long run by Mongolia's inbound tourism but not vice versa can be explained by the fact that the number of tourists visiting Mongolia would include China in their travels.

Considering Games with the broad definition proposed by Juul (Juul, 2010), consequences outside of the magic circle can be negotiated. This definition opens up the possibility to define serious games, games developed with an utilitarian goal in mind, in addition to fun. The entertaining and utilitarian objectives may however be contradictory, leading serious games to be, more often than not, less than optimal in at least one of the two dimensions. Another way to play with the boundaries of games is to consider pervasive games, which include alternate reality games, and crossmedia games (Montola, 2005). We question here the limit between game, play and toy in the context of a mixed reality serious game. ‘Pangu’ is a game designed for bachelor students, with biochemistry as the utilitarian objective, and the origin of life as a game theme. The students are asked to play the game on their smartphone, which in turn ask them to build molecules with a tangible balls-and-sticks model typically used in chemistry classes. Pictures taken from the models allow users to ‘scan’ these models and progress in the game. The use of the game was observed in four opportunities. An unanticipated observation is that, in addition to expected behaviors, some students used briefly the models like a toy rather than in the context of the game. It is therefore tempting to speculate that the pervasive nature of the game is blurring the game/non game boundary and, in the context of this serious game, opens a door for fun.

Energy storage systems will play a key role for individual users in the future smart grid. They serve two purposes: (i) handling the intermittent nature of renewable energy resources for a more reliable and efficient system, and (ii) preventing the impact of blackouts on users and allowing for more independence from the grid, while saving money through load-shifting. In this paper we investigate the latter scenario by looking at a neighbourhood of 25 households whose demand is satisfied by one utility company. Assuming the users possess lithium-ion batteries, we answer the question of how each household can make the best use of their individual storage system given a real-time pricing policy. To this end, each user is modelled as a player of a non-cooperative scheduling game. The novelty of the game lies in the advanced battery model, which incorporates charging and discharging characteristics of lithium-ion batteries. The action set for each player comprises day-ahead schedules of their respective battery usage. We analyse different user behavior and are able to obtain a realistic and applicable understanding of the potential of these systems. As a result, we show the correlation between the efficiency of the battery and the outcome of the game.

Pancreatic cancer stands out as one of the most lethal forms of malignancies, representing 2% of all cancer cases and contributing to 5% of cancer-related fatalities. Therefore, early detection of pancreatic cancer is crucial for enhancing treatment outcomes. Various hereditary cancer syndromes have been linked to an elevated risk of developing pancreatic cancer, suggesting potential benefits from surveillance strategies for individuals at risk. Presently, precision medicine employs PARP inhibitor therapy for pancreatic cancer patients carrying germline variants in Homologous Recombination Repair (HRR) genes. Our objective was to ascertain the occurrence of germline pathogenic/likely pathogenic variants in genes predisposing to cancer among pancreatic cancer patients. Methodologically, we analyzed 184 pancreatic cancer patients referred to our laboratory for genetic examination. Utilizing a Next-Generation Sequencing (NGS) approach, we scrutinized 52 genes implicated in hereditary cancer predisposition. Our findings revealed that 21% of the patients analyzed harbored germline pathogenic/likely pathogenic variants. Within this subset, 48% exhibited positive results in pancreatic cancer susceptibility genes, namely ATM (17%), BRCA1 (12%), BRCA2 (7%), CDKN2A (7%), and PALB2. Significantly, 64.3% of the pathogenic variants were associated with genes involved in the HRR pathway (ATM, BRCA1, BRCA2, BRIP1, CHEK2, FANCL, MRE11, PALB2, RAD50, RAD51B and RAD51C). In conclusion, our study underscores the significance of multigene genetic testing for pancreatic cancer patients, with 21% of individuals yielding findings linked to pancreatic or other cancer predisposition. Moreover, patients carried pathogenic/likely pathogenic variants in HRR genes, potentially benefiting from targeted therapies such as PARP inhibitors or platinum-based treatments.

The current recommendation to avoid non-steroidal anti-inflammatory drugs (NSAIDs) in management of dengue virus disease is, as it was adopted earlier in COVID-19, scientifically considered of very low to low certainty. In this perspective I argue, for the first time globally, basing on real-life practice that NSAIDs might be lifesaving in early management of dengue virus disease as I originally argued and proved rightful in COVID-19. Moreover, I also argue that the Egyptian immune-modulatory Kelleni’s protocol, including nitazoxanide as an integral component, can be safely and effectively used to early manage COVID-19, human metapneumovirus, dengue virus disease, Zika virus separate or co-infections similar to what has already been experienced and published with separate or conjoined infections with SARS CoV-2, influenza, RSV and Norwalk viruses without the need of prior serological or molecular investigations to determine the causative agent.

Chemical safety is related to public health, safety and environmental concerns, and the dangerous chemicals safety problem is becoming the one that people commonly pay attention to. Strengthening chemical safety supervision not only involves safe production, but also is an important link to maintain social safety. Most related studies focus on chemical safety under normal regulation, this paper addresses the perspective of ‘special rectification’ and ‘normal regulation’, and establishes an evolutionary game model between chemical enterprises and government supervision departments under different supervision modes. Based on the evolutionary game theory, this paper studies the evolution process of the two game players' strategy choices, and compares and analyzes the evolution, stability and equilibrium between the chemical safety and the behavior strategies of government regulatory departments. The model is effectively analyzed based on numerical simulation, and the results show that: Under the ‘special rectification’ mode, the strategic choice of chemical enterprises engaging in safety without investment depends on the difference between the benefits and costs of safety without investment; In the ‘normal regulation’ mode, the choice of its safety non-investment strategy depends on the difference between the cost of engaging in safety investment and the cost of safety non-investment; Increasing the government's punishment will encourage chemical enterprises to take safety investment behavior under the two supervision modes. Increasing the punishment has a significant impact on the safety investment behavior of chemical enterprises under the ‘normal regulation’ mode, but it has no significant impact on the behavior of chemical enterprises under the ‘special rectification’ mode. At the same time, increasing the punishment will inhibit the strict supervision behavior of the government. The research conclusion provides important decision support for government departments to effectively supervise the safety production of chemical enterprises.

TeamIndus’ Lunar Logistics vision includes multiple lunar missions over the coming years to meet requirements of science, commercial and efforts towards building readiness for crewed missions to Mars in the global exploration roadmap. TeamIndus is the only Indian team that participated in the Google Lunar X Prize. The challenge called for privately funded spaceflight teams to be the first to land a robotic spacecraft on the Moon, travel 500 meters, and transmit back high-definition video and images. The first mission is expected to have a net landed payload capacity of 50 kg. The prime objective is to demonstrate autonomous lunar landing, and operations for a Surface Exploration Rover - to collect data in the vicinity of the landing site. The rover is designed to execute the commands given by the operations manager autonomously. The rover software architecture relevant to the navigation and control is described in detail. The functional modes are defined to functionally distinguish the rover drive. Among the various operations performed by the rover are execution of simple drive steps through navigation and control algorithms. The kinematic model of the rover is studied in the hardware locomotion tests performed. The Kalman filter formulation to estimate the sensor bias and to get attitude estimate is discussed. The structural model and the frame definitions are described in a relevant section. A simple heading control algorithm is designed to control the heading direction of the rover according to the operations requirement. The path planning process and visual odometry processes are described. Finally the SIMULINK$^{\textregistered}$ model is tested on the prototype rover and the test experience results are discussed in the last section.

Alternative food sources are essential in both low-resource settings or during emergencies like abrupt sunlight reduction scenarios. Seaweed presents a promising option but requires investigation into the viability of unconventionally sourced ropes for harvesting. As such, a low-cost reliable method to test the tensile strength of rope is needed to validate alternative materials for use in harvesting seaweed. Unfortunately, commercial rope testing jigs alone range in price from several thousand to tens of thousands of dollars. To fill this need, this article reports on an open-source design for tensile strength rope testing hardware. The hardware design focuses on using readily available parts that can be both sourced from a hardware store and manufactured with simple tools to provide for the greatest geographic accessibility. The jig design, which can be fabricated for CAD$20 is two to three orders of magnitude less expensive than commercially available solutions. The jig was built and tested using a case study example investigating denim materials (of 1 5/8”, 3 1/4", 4 7/8”, 6 1/2", and 8 1/8” widths) as a potential alternative rope material for seaweed farming. Denim demonstrated strength up to 1.65kN for the widest sample and the jig demonstrated sufficient strength and stiffness for operating at forces below 4kN. The results are discussed and areas for future improvements are outlined to adapt the device to other circumstances and increase the strength of materials that can be tested.

TThe aim of the work is to present a newly developed stand and methodology of accelerated tests of fatigue strength of flexible propellers and fins. The paper presents the design and concepts of the measuring elements used in the research. Test methodology and water stressing were devel-oped. The mechanical properties of the following materials were presented: Arnitel EL-550 and QUEO 8230 with the addition of PP / EVA 50/50 (polypropylene / ethylene-vinyl acetate) - in the proportion of 70/30 (QUEO 8230 / additive). The constructed test stand and a new method of fa-tigue testing of biomimetic fins made it possible to determine the number of fin movement cy-cles under a given load, which may facilitate the determination of a warranty for a newly intro-duced product. The current consumption by the fin drive and video monitoring allowed for the selection of the range of the number of movement cycles and the determination of the moment of fatigue cracks until the fin structure loses its capacity. During the analysis, the test results showed that the fins made of Arnitel EL-550 did not show changes in the measured values of the current and the force generated by the fin. For a fin made of QUEO 8230, at 35,000 cycles, there is a decrease in the current and force consumed, which indicates the initiation of fatigue damage to the fin. The performed tests can be the basis for the development of standards for the fatigue strength of flexible propellers.

IoT is a fast growing technology that has Promising potential for shaping our future. In this fast growing world of IoT, IoT systems are released without proper testing which effect its quality and does not guarantee user satisfaction. Different testing methodologies are carried out to ensure Quality assurance of IoT before releasing it to the market. In this paper we have reviewed different testing techniques using AI and different tools to ensure Quality of IoT. In this paper we have also reviewed different IoT challenges related to its quality.

The nondestructive testing of reinforced concrete chimneys, especially the high ones, is an important element of the assessment of their condition, making it possible to forecast their safe service lifespan. Industrial chimneys are often exposed to the strong action of acidic substances – they are adversely affected by the flue gas condensate on the inside and by acid precipitation on the outside. Initially, this results in the corrosion of the shell concrete and then in the corrosion of the reinforcing steel. During the service life of such chimneys their condition should monitored in order to prevent structural failures and indicate the most endangered parts of the structure. Owing to thermographic surveys one can monitor the hazards leading to the degradation of the chimney structure, which is particularly vital when due to the character of the production process the chimney cannot be put out of operation. The methods for the interpretation of results from thermovision studies to determine the safety and durability of industrial chimneys are shown.

High throughput multiplex serological systems enable the small developer to set up tests at small cost, for microbes for which there are no commercial tests, and for aspects which have not been addressed by them. An example is testing for Zika and Tick Borne Encephalitis virus antibodies, where antigenic cross-reactions make diagnosis problematic. Our technique variant, Suspension Multiplex Immunoassay (SMIA) allows many samples to be tested for antibodies to many antigens in a short time. Computational compensation for cross-reactions is possible if a SMIA panel contains most of the potentially cross-reacting antigens. Using antibody avidity and pattern of reactivity to whole virus and nonstructural protein, antibodies due to vaccination and infection, respectively, as well as probable degree of protection, can be determined with high throughput. These multiplex techniques hold great promise for future diagnostic development. Theoretically, even large scale serological monitoring, like blood donor pathogen testing, could be done inexpensively and rationally with multiplex serology developed in house. However, the quality control demands are steep and in most cases out of scope for a single laboratory. There remain however a number of clinical applications where in house multiplex serology can be performed with adequate quality control under high throughput conditions.