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Preprint ARTICLE | doi:10.20944/preprints201901.0212.v1

One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3

Xueming Chen, Yuhuan Chen, Chunguo Liu, Xiaojun Li, Hongyu Liu, Xiaofei Bai, Ming Liu, Yun Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DHAV-1; DHAV-3; Phylogenetic analysis; One-tube RT-PCR; Simultaneously

Online: 22 January 2019 (11:08:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1468.v1

Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR

Claudio A. Henriquez, Natalia Riquelme, Alejandra Larach, Ximena Besoain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Virus-viroid detection; Mixed infections; Comparison of diagnostic methods; CTV; HSVd; Bioamplification

Online: 19 January 2024 (07:52:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0991.v1

Potential Performance of Two New RT-PCR and RT-q PCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes Allowing Efficient Entomological Surveillance of Both Vector-Borne Diseases

Michel Kiréopori Gomgnimbou, Louis robert wendyam BELEM, Etienne Bilgo, Miriam Félicité Amara, Zouera Laouali, Ali Ouari, Toussaint Bayala, Kobo Gnada, Raymond Kharlis Yao, Moussa Namountougou, Ibrahim Sangaré

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: multiplex rt-pcr; multiplex rt-qpcr; molecular detection; dengue virus; chikungunya virus; mosquitoes; Burkina Faso

Online: 12 July 2024 (08:43:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Working Paper BRIEF REPORT

Positive Rate Is Useful to Evaluate the Sufficiency of RT-PCR Test Availability for COVID-19

Sadao Suzuki

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; RT-PCR test; pre-test probability; positive rate

Online: 5 May 2020 (06:04:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1094.v1

Development and Evaluation of an In-House Real-Time Rt-Pcr Targeting nsp10 Gene for SARS-CoV-2 Detection

Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; nsp10; real-time RT-PCR; diagnostic

Online: 20 February 2024 (06:07:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints201702.0011.v1

Simulation Analysis of Solution Transportation Absorption Chiller with the Capacity from 25 RT to 1000 RT

Koji Enoki, Fumi Watanabe, Seigo Tanaka, Atsushi Akiwawa, Toshitaka Takei

Subject: Engineering, Energy And Fuel Technology Keywords: absorption chiller; thermal energy transportation; solution transportation; ammonia-water; COP; simulation

Online: 4 February 2017 (07:49:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1057.v1

From One Heath to One Sustainability. The Role of Contagious Mastitis Pathogens in Decreasing the Dairy Herd Sustainability

Francesca Zaghen, Valerio Massimo Sora, Giampaolo Zanirato, Alfonso Zecconi

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: sustainability; milk; contagious pathogens; animal welfare; One Health

Online: 14 August 2024 (14:16:48 CEST)

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Economic, social, and environmental sustainability are the results of efforts aiming to improve all aspects of milk production, respecting animal welfare and improving herd health. An epidemi-ological study was designed to assess the role of contagious pathogens (S. aureus and S.agalactiae) in a cohort of 120 dairy herds located in the southern regions of Italy. Milk quality was assessed using certified methods, and the prevalence of mastitis pathogens in bulk tank milk was determined using quantitative polymerase chain reaction. Welfare scores were assessed using a scoring card that have more than 100 items, including animal-based measurements. Statistical analyses were performed using general lineal model and logistic regression procedures. The results showed that S. aureus had a significant negative effect on the amount of milk components delivered to the dairy plant, and on the level of welfare. Whereas, the presence of S.agalactiae did not show any significant association. The major risk factors associated with the presence of S.aureus were also identified to help prioritise control programs. These results support the “One Sustainability” approach im-plying that an increase of animal productivity is related to the improvement of animal health and welfare and potentially leading to the mitigation of greenhouse gas emissions.Economic, social, and environmental sustainability are the results of efforts aiming to improve all aspects of milk production, respecting animal welfare and improving herd health. An epidemi-ological study was designed to assess the role of contagious pathogens (S. aureus and S.agalactiae) in a cohort of 120 dairy herds located in the southern regions of Italy. Milk quality was assessed using certified methods, and the prevalence of mastitis pathogens in bulk tank milk was determined using quantitative polymerase chain reaction. Welfare scores were assessed using a scoring card that have more than 100 items, including animal-based measurements. Statistical analyses were performed using general lineal model and logistic regression procedures. The results showed that S. aureus had a significant negative effect on the amount of milk components delivered to the dairy plant, and on the level of welfare. Whereas, the presence of S.agalactiae did not show any significant association. The major risk factors associated with the presence of S.aureus were also identified to help prioritise control programs. These results support the “One Sustainability” approach im-plying that an increase of animal productivity is related to the improvement of animal health and welfare and potentially leading to the mitigation of greenhouse gas emissions.

Preprint ARTICLE | doi:10.20944/preprints202409.1366.v1

Cycle Threshold Values of SARS-CoV-2 RT-PCR During Outbreaks in Nursing Homes: A Retrospective Cohort Study.

Juan Carlos Gasco-Laborda, Maria Gil-Fortuño, Maria Dolores Tirado-Balaguer, Noemi Meseguer-Ferrer, Oihana Sabalza-Baztán, Óscar Pérez-Olaso, Iris Gómez-Alfaro, Sandrine Poujois-Gisbert, Noelia Hernández-Pérez, LLedó Lluch-Bacas, Viorica Rusen, Alberto Arnedo-Pena, Juan Bautista Bellido-Blasco

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: SARS-CoV-2; PCR; cycle threshold; infections; nursing homes; outbreaks; retrospective cohort

Online: 18 September 2024 (11:47:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0321.v1

How to Improve Surveillance Program for Shiga Toxin-Producing E. coli (STEC): Gap Analysis and Pilot Study

Valerio Massimo Sora, Francesca Zaghen, Alfonso Zecconi

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: E. coli; STEC; epidemiology; surveillance; molecular biology; PCR; dairy; bovines.

Online: 3 April 2024 (16:33:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0859.v1

Comparing the Performance of Automatic Milking Systems through Dynamic Testing Also Helps to Identify Potential Risk Factors for Mastitis

Stefano Milanesi, Dario Donina, Viviana Chierici Guido, Francesca Zaghen, Valerio Massimo Sora, Alfonso Zecconi

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: milking machine; automatic milking system; milking dynamic test; bimodality; mastitis

Online: 11 September 2024 (16:59:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0423.v1

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Mikiko Soejima, Yoshiro Koda

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Alu-mediated nonhomologous recombination; FUT2; sedel2; c.375A>G; c.385A>T; c.571C>T; Polynesian population

Online: 17 April 2023 (07:43:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0737.v1

Integrated Environmental Design and Robotics Workflow for Double-Skin Facade Perforation Onsite

Ahmed Khairadeen Ali, One Jae Lee

Subject: Engineering, Automotive Engineering Keywords: double-skin facade perforation; environmental design; robotics in construction; VR/AR for monitoring; digital twin in construction

Online: 31 May 2021 (10:42:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1127.v1

Machine Learning Application to Brain Diseases Detection

Aleksandar Simović, Maja Lutovac-Banduka, Kristina Davidović, Snežana Lekić

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; application; classification; brain diseases; detection

Online: 17 November 2023 (15:32:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0439.v1

SARS-CoV-2 Detection in Fecal Samples in Sym-asymptotic Patients with Typical Findings of COVID-19 on Ag-RDT and SARS-CoV-2 RT-PCR Tests

Richard Odongo Magwanga, Benson Onyango, Alfred Ochieng, Shehu Shagari Awandu, Pamela Were, Joy Nyangasi Kirungu, Marceline Adhiambo Oloo, Erick Muok, Stephen Munga, Fredrick Ogutu, Angeline Orwa, Fredrick Okumu, Clayton Onyango, Dorothy Awuor, Francis Orata, Justine Ochieng’-Midega, Bonface Oure, Regina Nyunja, Fredrick Ade, Sharley Indechi, Erick Okuto, Benson Estambale

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: COVID19; SARS-CoV-2 virus; Oro-nasopharyngeal; fecal; vaccination; asym-symptomatic; rapid diagnostic test

Online: 28 September 2022 (11:51:21 CEST)

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Coronavirus is a disease caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged as a global pandemic in 2019 from Wuhan, China. Since its emergence, it has caused immense suffering to human life, 6.27 million lives have been lost, movement curtailed and social dynamics disrupted. The golden standard for getting samples for SARS-CoV-2 detection is through oral- nasopharyngeal swab, this method of sample collection is invasive and uncomfortable, thus stigmatized the general population, and thereby impeded the progress of controlling the spread through mass testing. Being a contact disease, mechanisms to encourage mass testing is key to reduce the spread. This study thus developed a complimentary sample type to test for SARS-CoV-2, the use of human feces. Fecal samples were collected from 100 asym-symptomatic individuals suspected to be infected with COVID-19, virus RNA was then extracted and profiled through Real Time Polymerase Chain Reaction (RT-qPCR). The antigen rapid diagnostic test revealed high positivity rate of 44%, but the real time polymerase chain reaction results on nasopharyngeal and fecal samples revealed a significant variation, high number of the patients tested positive with stool samples compared to the nasopharyngeal swabs, with 43 and 37%, respectively. SARS-CoV-2 virus was detected in both symptomatic and asymptomatic individuals; however, the symptomatic registered a higher positivity of 25% compared to 20% among the asymptomatic patients. Vaccination only lowered the risk of infection, fully and partially vaccinated lowered the infection level to 10% compared to 20% among the unvaccinated. Finally, gender parity in relation to COVID19 was evaluated, more females (56%) compared to males were recruited in this study, out of which (20; 43.4%) were positive, and 26 (56.6%) were negative based on fecal RT-qPCR outcomes. Based on the outcome of this study, rapid diagnostic test (Ag-RDT) however cheap and or fast does not provide accurate information, moreover, the virus does not stay longer within the Oro-nasopharyngeal region, thus the invalid or negative results, thus use of feces should be adopted as a confirmatory test to ascertain the COVID19 status of an individual.

Preprint ARTICLE | doi:10.20944/preprints202304.0451.v1

One Single Tube Reaction of Aptasensor-Based Magnetic Sensing System for Selectively Fluorescent Detection of VEGF in Plasma

Hwang-Shang Kou, Shao-Tsung Lo, Chun-Chi Wang

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: VEGF; Aptasensor; Streptavidin magnetic bead; Plasma; Hybridization probe

Online: 17 April 2023 (10:43:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0387.v1

Acquisition of Humoral Immune Responses in Convalescent Japanese People with SARS-CoV-2 (COVID-19) Infection in 2021

Koshiro Monzen, Takanori Watanabe, Toshihiro Okabe, Hisakuni Sekino, Hironori Nakagami, Ryuichi Morishita

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2; Convalescent serum; Neutralizing antibody

Online: 4 August 2023 (12:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0715.v1

Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of Setaria tundra (Issaitshikoff &Amp; Rajewskaya, 1928) in Serbia

Sara Šiljegović, Theo Mouillaud, Davy Jiolle, Dušan Petrić, Aleksandra Ignjatović-Ćupina, Ana Vasić, Christophe Paupy, Mihaela Kavran

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Dirofilaria sp.; Setaria tundra; mosquito surveillance; Cox1 gene

Online: 11 April 2024 (04:07:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0086.v1

Double-Skin Facade Perforation Workflow Onsite Using Robot Arm

Ahmed Khairadeen Ali, One Jae Lee

Subject: Engineering, Mechanical Engineering Keywords: double-skin facade perforation, environmental design, robotics in construction, VR/AR for monitoring, digital twin in construction.

Online: 6 May 2021 (13:11:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0690.v1

Facade Style Mixing using Artificial Intelligence for Urban Infill

AHMED KHAIRADEEN ALI, One Jae Lee

Subject: Arts And Humanities, Architecture Keywords: : Artificial Intelligence; Generative Adversarial Network; Machine Learning; Computationl Design; Urban infill; Facade design

Online: 26 April 2021 (20:20:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0446.v1

Symbolic Analysis of Classical Neural Networks for Deep Learning

Maja Lutovac-Banduka, Igor Franc, Vladimir Milićević, Nemanja Zdravković, Nikola Dimitrijević

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; visual programming language; closed-form expression; theoretical justification; feature extraction; complexity; optimization and validation

Online: 7 November 2023 (11:25:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints201612.0033.v1

Perceived Credibility, Evaluation of Outcome and Attitude towards Behavioural Change in 360-Degree Assessment

Peter Cheng

Subject: Social Sciences, Behavior Sciences Keywords: 360-degree assessment; perceived credibility; evaluation of outcome; theory of reasoned action; attitude formation

Online: 6 December 2016 (11:09:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1908.v1

Deep Dive Into the DNA Polymerase Through Insilico Analysis: An Information to Get Better PCR Enzyme From the Ancient One

Debanjan Mitra

Subject: Biology And Life Sciences, Other Keywords: Polymerase chain reaction; DNA polymerases; Intra-protein interactions; Protein satbility

Online: 27 June 2023 (12:46:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0030.v1

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi, Hussein Alburkat, Taisir Abdulelah Kadhim, Ammar M S Al-Helli, Mustafa Hamza Ghazi, Yasir Abdulameer N. Aldabbagh, Reem Numaan Talib, Sama Hussein Ali, Noor Hameed Imran, Ruaa Almohsen, Turki Muftin Saad, Pallavi Mandave

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: PCR; Salmonella Typhi; Widal Test; BHI

Online: 1 November 2022 (12:43:58 CET)

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Preprint REVIEW | doi:10.20944/preprints202406.0865.v1

When Size Matters: New Insights on How Seed Size Can Contribute to Plant Developmental Success

Alesssandra Boccaccini, Sara Cimini, Hira Kazmi, Andrea Lepri, Chiara Longo, Riccardo Lorrai, Paola Vittorioso

Subject: Biology And Life Sciences, Plant Sciences Keywords: Seed size; germination; cell wall; environment; development; Arabidopsis thaliana; Oryza sativa

Online: 13 June 2024 (03:14:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1411.v1

Therapeutic Effect of Oral Diet Training in Indwelling Nasogastric Tube Patients with Prolonged Dysphagia

Byung-chan Choi, Sook Joung Lee, Eunseok Choi, Sangjee Lee, Jungsoo Lee

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Nasogastric tube; Deglutition disorder; Chronic dysphagia; Oral training

Online: 20 June 2024 (13:10:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0458.v1

Effect of Structural Parameters and Materials on the Mechanical Characteristics of Expansion Tube

Zizhen Qi, Yuwu Zhang, and Yuliang Lin

Subject: Engineering, Mechanical Engineering Keywords: expansion tube; mechanical properties; energy absorption; parent material comparison; plastic deformation

Online: 20 July 2020 (08:10:40 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.1089.v1

White Paper Textile Fibre Recycling Technologies

Birgit Stubbe, Stijn Van Vrekhem, Sofie Huysman, Rémi G. Tilkin, Isabel De Schrijver, Myriam Vanneste

Subject: Chemistry And Materials Science, Paper, Wood And Textiles Keywords: textile recycling; fibre-to-fibre recycling; textile sorting; textile disassembly; digital textile platforms

Online: 17 November 2023 (08:49:16 CET)

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Preprint REVIEW | doi:10.20944/preprints202406.0782.v1

One-Dimensional Relativistic Self-Gravitating Systems

Robert B. Mann

Subject: Physical Sciences, Theoretical Physics Keywords: Self-Gravitating Systems; Lower-Dimensional Gravity; Relativistic Chaos

Online: 13 June 2024 (03:40:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1591.v1

Subcrestal versus Bone Level One Stage Implants: Early Bone and Soft Tissues Modification. One Year Randomized Clinical Trial.

Magda Mensi, Eleonora Scotti, Stefano Calza, Stefano Salgarello, Annamaria Sordillo, Matteo Zola, Diego Lops

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: Sub-crestal implants, bone loss, one time abutment, conical connection, mucosal tunnel depth

Online: 28 February 2024 (11:31:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202406.0874.v1

Antimicrobial Resistance in Aquaculture: Risk Mitigation within One Health Context

Milan Milijasevic, Slavica Veskovic-Moracanin, Jelena Babic Milijasevic, Jelena Petrovic, Ivan Nastasijevic

Subject: Biology And Life Sciences, Food Science And Technology Keywords: antimicrobial resistance, aquaculture, fisheries, risk mitigation, One Health

Online: 13 June 2024 (09:04:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0509.v1

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Antonio Tiberini, Ariana Manglli, Anna Taglienti, Ana Vučurović, Jakob Brodaric, Luca Ferretti, Marta Luigi, Andrea Gentili, Nataša Mehle

Subject: Biology And Life Sciences, Plant Sciences Keywords: ToBRFV; ToMMV; Solanum lycopersicum; Capsicum annum; leaves detection; seeds detections; performance criteria

Online: 31 December 2021 (11:12:18 CET)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints201611.0098.v1

Nonlinear Vibration Response of a Rectangular Tube with a Flexible End and Non-Rigid Acoustic Boundaries

Y. Y. Lee

Subject: Engineering, Mechanical Engineering Keywords: nonlinear plate vibration; structural acoustics; harmonic balance method

Online: 18 November 2016 (10:13:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1957.v1

Mild Phenotypes of Gyrate Atrophy in a Carrier with One Variant Allele of OAT

Yuqiao Ju, Xiao Li, Fengjuan Gao, Qing Chang, Yuan Zong, Xin Huang

Subject: Medicine And Pharmacology, Ophthalmology Keywords: gyrate atrophy of the choroid and retina (GACR); OAT gene; carrier; allele; autosomal recessive

Online: 30 April 2024 (07:28:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0585.v1

Remote Indoor Construction Progress Monitoring Using Extended Reality

Ahmed Khairadeen Ali, One Jae Lee, DoYeop Lee, Chansik Park

Subject: Engineering, Automotive Engineering Keywords: virtual reality; point cloud data; building information modeling; work inspection; progress monitoring; quality assurance inspection.)

Online: 23 November 2020 (14:03:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0123.v1

One-Step Fabrication of Composite Hydrophobic Electrically Heated Graphene Surface for Anti-Icing

Mian Zhong, Shichen Li, Hongyun Fan, Huazhong Zhang, Yong Jiang, Jinling Luo, Liang Yang

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: LIG; Hydrophobic surface; electrically heated materials; one-step fabrication; contact angle

Online: 2 July 2024 (13:28:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1629.v1

Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications

Gustavo Henrique Doná Rodrigues Almeida, Raquel Souza da Silva, Mariana Sversut Gibin, Victoria Helen de Souza Gonzaga, Henrique dos Santos, Rebeca Piatniczka Igleisa, Leticia Alves Fernandes, Iorrane Couto Fernandes, Thais Naomi Gonçalves Nesiyama, Franciele Sato, Mauro Luciano Baesso, Luzmarina Hernandes, Jaqueline de Carvalho Rinaldi, Flavio Vieira Meirelles, Claudete S. Astolfi-Ferreira, Antonio José Piantino Ferreira, Ana Claudia Oliveira Carreira

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: uterine tube; extracellular matrix; reproduction; decellularization; biomaterial

Online: 24 May 2024 (11:56:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0262.v1

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Ju Huang, Ligong Zhai, Junyin Wang, Xiaotian Sun, Baoshi Wang, Zhaohui Wei

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Pseudomonas aeruginosa; Pseudomonas fragi; simultaneous detection; droplet digital PCR

Online: 3 April 2024 (16:17:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0456.v1

Benefits of a Switch From Intermittently Scanned Continuous Glucose Monitoring (isCGM) to Real-time (rt) CGM in Diabetes Type 1 Suboptimal Controlled Patients in Real-life: A One-year Prospective Study

Yannis Préau, Sébastien Galie, Pauline Schaepelynck, Martine Armand, Denis Raccah

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Real-time continuous glucose monitoring; Intermittently scanned continuous glucose monitoring; Type 1 diabetes; Glucose variability; Hypoglycemia; Insulin resistance

Online: 20 July 2021 (14:58:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0604.v2

RT-qPCR Diagnostics: The “Drosten” Sars-Cov-2 Assay Paradigm

Stephen Bustin, Sara Kirvell, Jim F Huggett, Tania Nolan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID-19; reverse transcription; qPCR; SARS-CoV-2; molecular diagnosis

Online: 3 August 2021 (15:34:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1440.v1

Performance Analysis of New One-Piece Iron Roughneck and Its Spinning Mechanism

Yong bai Sha, Dong he Han, Dong hu Chen, Cong zhi Liu

Subject: Engineering, Mechanical Engineering Keywords: integrated iron roughneck; clamp body structure; contact theory; rolling friction theory; Floating device; dynamics simulation experiment

Online: 17 July 2024 (12:36:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0122.v1

Target-Driven Tissue-Agnostic Drug Approvals; a New Path of Drug Development

Kyaw Z Thein, Yin M Myat, Byung S Park, Kalpana Panigrahi, Shivaani Kummar

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Tissue agnostic drug development; Pembrolizumab; Microsatellite instability-high (MSI-H); Tumor mutational burden-high (TMB-H); Dostarlimab-gxly; Larotrectinib; Entrectinib; Neurotrophic tyrosine kinase (NTRK); Dabrafenib and trametinib; V-Raf Murine Sarcoma Viral Oncogene Homolog B V600E (BRAFV600E); Selpercatinib; Rearranged during transfection (RET); Precision oncology.

Online: 3 June 2024 (16:08:38 CEST)

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The regulatory approvals of tumor agnostic therapies have led to reevaluation of the drug development process. Conventional models of drug development are histology-based. On the other hand, tumor agnostic drug development of a new drug (or combination) focuses on targeting a common genomic biomarker in multiple cancers, regardless of histology. Basket-like clinical trials with multiple cohorts allow clinicians to evaluate pan-cancer efficacy and toxicity. There are currently 8 tumor agnostic approvals granted by the Food and Drug Administration (FDA). This includes two immune checkpoint inhibitors, and 5 targeted therapy agents. Pembrolizumab is an anti-PD-1 antibody that was the first FDA approved tumor agnostic treatment for unresectable or metastatic microsatellite instability-high (MSI-H) or deficient mismatch repair (dMMR) solid tumors in 2017. It was later approved for tumor mutational burden-high (TMB-H) solid tumors, although the TMB cut-off used is still debated. Subsequently, in 2021, another anti-PD-1 antibody, Dostarlimab was also approved for dMMR solid tumors in the refractory setting. Patients with fusion-positive cancers are typically difficult to treat due to their rare prevalence and distribution. Gene rearrangements or fusions are present in a variety of tumors. neurotrophic tyrosine kinase (NTRK) fusions are present in a range of pediatric and adult solid tumors in varying frequency. Larotrectinib and entrectinib were approved for neurotrophic tyrosine kinase (NTRK) fusion-positive cancers. Similarly, selpercatinib was approved for Rearranged During Transfection (RET) fusion-positive solid tumors. FDA approved the first combination therapy of dabrafenib, a B-Raf Proto-Oncogene Serine/Threonine Kinase (BRAF) inhibitor, plus trametinib, a mitogen-activated protein kinase (MEK) inhibitor for patients 6 months or older with unresectable or metastatic tumors (except colorectal cancer) carrying BRAFV600E mutation. The most recent FDA tumor agnostic approval is of fam-trastuzumab deruxtecan-nxki (T-Dxd) for HER2-positive solid tumors. It is important to identify and expeditiously develop drugs that have the potential to provide clinical benefit across tumor types.

Preprint ARTICLE | doi:10.20944/preprints202408.1358.v1

One Health Response for Rift Valley Fever Outbreak in Sudan

Ayman Ahmed, Yousif Ali, Nahid A. Ibrahim, Sana I. Mohamed, Jakob Zinsstag, Emmanuel Edwar Siddig, Nouh Saad Mohamed, Claude Mambo Muvunyi

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Zoonotic diseases; Arboviruses; Emerging Infectious Disease; Epidemic; Epizootic; Haemorrhagic fevers; Health security; Pandemic preparedness; prevention and response

Online: 19 August 2024 (14:13:51 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0737.v1

Ammonia Can be Currently Considered as one of the Best Green Energy Ally

Rubén González, Xiomar Gómez

Subject: Engineering, Chemical Engineering Keywords: economy decarbonization; alternative fuels; CO2 emission reduction; engines

Online: 13 March 2024 (12:00:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1123.v2

Visual Imitation Learning from One-Shot Demonstration for Multi-Step Robot Pick-and-Place Tasks

Shuang Lu, Christian Haerdtlein, Johannes Schilp

Subject: Computer Science And Mathematics, Robotics Keywords: Visual imitation learning; Robot; One-shot demonstration

Online: 16 September 2024 (10:03:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.2036.v1

Electrosurgery for the Gynaecologist: Cutting and Coagulating with One Instrument and One Setting

Philippe R Koninckx, Anastasia Ussia, Bedayah Amro, Jörg Keckstein, Simon Keckstein, Romeo Armando, Arnaud Wattiez

Subject: Medicine And Pharmacology, Surgery Keywords: laparoscopic surgery; electrosurgery

Online: 29 January 2024 (13:22:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints201611.0058.v1

Effects of Domain Boundaries on the Diffraction Patterns of One Dimensional Structures

Frederic Timmer, Joachim Wollschläger

Subject: Physical Sciences, Condensed Matter Physics Keywords: spot-profile analysis; one dimensional physics; low energy electron diffraction; binary surface technique; supercell model; domain boundary

Online: 10 November 2016 (15:50:29 CET)

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Motivated by diffraction experiments on the

(2 \sqrt{3} \times \sqrt{3}) R 30^{\circ}

reconstructed Si(111) due to deposition of rare earth elements (Dy, Tb) and silicide formation we analyse the splitting and non-splitting of superstructure spots. For this purpose, we model diffraction patterns for one dimensional structures generated by the binary surface technique and use supercell models to keep the analysis simple. Diffraction pattern are calculated in the framework of the kinematical diffraction theory and they are analyzed as a function of the domains and domain boundaries. Basic properties of the diffraction pattern are analyzed for model systems of a two-fold and a three-fold periodicity. The rules derived from these calculations are applied to the "real-world" system of Si(111)-(

(2 \sqrt{3} \times \sqrt{3}) R 30^{\circ}

)-RESi

2

(RE = Dy or Tb). Depending on the combination of domains and domain boundaries of different types a plethora of different features are observed in the diffraction patterns. These are analyzed to determine the sizes of both domain boundaries and domains from experimentally observed splitting of specific superstructure spots.

Preprint ARTICLE | doi:10.20944/preprints202104.0299.v1

Optimizing Droplet Digital PCR for Environmental Samples

Vasilis Kokkoris, Eric Vukicevich, Andrew Richards, Corrina Thomsen, Miranda Hart

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ddPCR, droplet digital PCR, environmental samples, environmental DNA, soil microbiology

Online: 12 April 2021 (12:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0203.v1

Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken

Subject: Biology And Life Sciences, Insect Science Keywords: Tungiasis; Tunga penetrans; molecular entomology; DNA isolation; Phusion® polymerase; FIREpol® Taq polymerase; low-cost PCR,

Online: 10 November 2022 (11:19:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints201809.0244.v1

A Single Tube Contactor for Testing Membrane Ozonation

Garyfalia A. Zoumpouli, Robert Baker, Caitlin M. Taylor, Matthew J. Chippendale, Chloë Smithers, Sean H.S. Xian, Davide Mattia, Y. M. John Chew, Jannis Wenk

Subject: Engineering, Chemical Engineering Keywords: ozonation; membranes; polydimethylsiloxane; mass transfer; wastewater treatment; peroxone

Online: 13 September 2018 (15:16:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1707.v1

Progressive Collapse Resistance Assessment of Multi-Column Frame Tube Structure with Assembled Truss Beam Composite Floor under Different Column Demolition Conditions

Rongguo Zhao, Guangfei Chen, Zaihua Zhang, And Wei Luo

Subject: Engineering, Civil Engineering Keywords: progressive collapse resistance; multi-column frame tube structure; truss beam composite floor; nonlinear dynamics; alternate load path method

Online: 27 November 2023 (13:16:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202004.0216.v1

Development of a Field-deployable RT-qPCR Workflow for COVID-19 Detection

Raphael Nyaruaba, Bo Zhang, Caroline Muema, Elishiba Muturi, Greater Oyejobi, Jin Xiong, Bei Li, Zhengli Shi, Caroline Mwaliko, Junping Yu, Xiaohong Li, Hongping Wei

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: COVID-19; SARS-CoV-2; field work; community; diagnosis; rapid detection; inactivation; RT-qPCR

Online: 14 April 2020 (08:41:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.2243.v1

Non-Obstructive Coronary Artery Disease and Atrial Fibrillation: A Complex Interplay with One Begets Another

Alexandru-Florinel Oancea, Paula Cristina Morariu, Ana Maria Buburuz, Ionela-Larisa Miftode, Radu Stefan Miftode, Ovidiu Mitu, Alexandru Jigoranu, Diana-Elena Floria, Amalia Timpau, Andrei Vata, Claudia Plesca, Gina Botnariu, Alexandru Burlacu, Dragos -Viorel Scripcariu, Mitea Raluca, Magdalena Cuciureanu, Daniela Maria Tanase, Irina Iuliana Costache-Enache, Mariana Floria

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: coronary ischemia; non-obstructive coronary artery disease; INOCA; MINOCA; ANOCA; atrial fibrillation

Online: 30 July 2024 (10:00:57 CEST)

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Working Paper ARTICLE

All for One and One for All: The COVID-19 Experience and Global Pandemic Preparedness

Titilope Olanipekun

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; globalization; health systems; pandemic; preparedness

Online: 30 August 2020 (11:38:30 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1182.v1

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Laura Sancha Domínguez, Ana Cotos Suárez, María Sánchez Ledesma, Juan Luis Muñoz Bellido

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: dPCR; digital PCR; infection; bacteria; virus; parasites

Online: 20 March 2024 (08:24:31 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0609.v1

Lung NETs and GEPNETs: One Cancer with Different Origins or Two Distinct Cancers?

Georgios Evangelou, Ioannis Vamvakaris, Anastasia Papafili, Maximilian Anagnostakis, Melpomeni Peppa

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Lung NETs, neuroendocrine tumors, typical carcinoid, atypical carcinoid, pulmonary NETs, EGFR, DLL3, immunotherapy

Online: 12 February 2024 (09:53:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202207.0288.v1

Design, Construction and Characterization of Sealed Tube Medium Power CO₂ Laser System

Muddasir Naeem, Tayyab Imran, Mukhtar Hussain, Arshad Saleem Bhatti

Subject: Physical Sciences, Applied Physics Keywords: ZEMAX; Thermoelectric coolers; Flyback transformer; High tension pump source; Penetration depth

Online: 19 July 2022 (13:28:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202112.0478.v1

Peripheral Nerve Injury Treatments and Advances: One Health Perspective

Bruna Lopes, Patrícia Sousa, Rui Alvites, Mariana Branquinho, Ana Sousa, Carla Mendonça, Luís Miguel Atayde, Ana Lúcia Luís, Artur S. P. Varejão, and Ana Colette Maurício

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Mesenchymal stem cells; Nerve Guide Conduits; Nerve recovery; One Health; Peripheral nerve injury; Secretome

Online: 30 December 2021 (07:35:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1962.v3

Analytical Solution of a Forced Vibrating Tube Transporting Fluid

Moussa Tembely

Subject: Engineering, Mechanical Engineering Keywords: Tube conveying fluid; forced vibration; Analytical solution

Online: 5 June 2024 (10:48:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0946.v1

Synthetic Ecosystems: From the Test Tube to the Biosphere

Ricard Sole, Victor Maull, Daniel R. Amor, Jordi Pla Mauri, Nuria Conde-Pueyo

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: synthetic biology; ecological engineering; climate change; ecospheres; life support systems

Online: 14 May 2024 (09:40:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1328.v1

Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of Bacteroides fragilis

Bakhtiyar Mahmood, Anna Paunkov, Malgorzata Kupc, Katalin Burián, Erzsébet Nagy, David Leitsch, József Sóki

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Bacteroides, metronidazole, nim, resistance mechanism, RT-qPCR

Online: 17 January 2024 (13:06:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202102.0525.v1

Exploring New Scaffolds for the Dual Inhibition of HIV-1 Rt Polymerase and Ribonuclease Associated Functions

Rita Meleddu, Angela Corona, Simona Distinto, Filippo Cottiglia, Serenella Deplano, Lisa Sequeira, Daniela Secci, Alessia Onali, Erica Sanna, Francesca Esposito, Italo Cirone, Francesco Ortuso, Stefano Alcaro, Enzo Tramontano, Peter Matyus, Elias Maccioni

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: HIV-RT; ribonuclease H; dual inhibitors; docking; putative binding

Online: 23 February 2021 (15:51:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.1735.v1

Return to Work After Breast Cancer. A One-Year Mixed-Method Study

Nicola Magnavita, Igor Meraglia, Daniela Andreina Terribile

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: disability management; longitudinal study; welfare; sleep, anxiety; depression; fatigue; work organization; barriers; facilitators; workplace

Online: 26 June 2024 (05:09:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0239.v1

An Immunoinformatics Approach for Identifying and Designing Conserved Multi-Epitope Vaccines for Coronaviruses

Yu Chuan Ong, Bimo Ario Tejo, Wei Boon Yap

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; coronavirus; universal vaccine; peptide; cell-mediated immunity

Online: 3 September 2024 (18:08:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1390.v1

Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection

Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: droplet Digital PCR; canine distemper virus; molecular diagnosis

Online: 18 September 2024 (10:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1177.v1

A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis

Thaís Ellen Chaves Gomes, Victor Coutinho Bastos, Douglas Boniek, Mário Romañach, Fernanda Faria Rocha, Roberta Rayra Martins Chaves, Ricardo Santiago Gomez

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Aspergillosis; Aspergillus; PCR; Diagnosis

Online: 15 August 2024 (14:30:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0326.v1

Molecular Characterization of Termitomyces Protoclones through PCR and RFLP Based DNA Typing

Pijush Mallick, Sobhan Roy Chowdhury, Dipanwita Roy, Subhash Kanti Roy, Samir Ranjan Sikdar

Subject: Biology And Life Sciences, Horticulture Keywords: DNA markers; edible mushroom; genome stability; protoplast regeneration

Online: 25 April 2018 (08:26:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2067.v1

One Year Follow-up of a 4-Year-Old Caucasian Girl Diagnosed with Stage IV Grade C Localized Periodontitis

Radu-Andrei Moga, Cristian Doru Olteanu

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: juvenile aggressive periodontitis; periodontal pocket; bone loss; hypophosphatasia; diagnosis; temporary dentition

Online: 25 July 2024 (14:56:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0194.v1

Optimization of Spray Tower Gas Scrubbers for Water-Tube Boilers

Edgar Italo Mendoza Haro, Jaime Camacho, Carla Mendoza, Xavier Mendoza

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: Water-tube boiler; fog eliminator; gas scrubber; particulate matter

Online: 5 September 2023 (03:04:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.2065.v1

A Synthetic Microbial Daisyworld: Planetary Regulation on the Test Tube

Victor Maull, Jordi Pla, Nuria Conde, Ricard Sole

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Daisyworld; homeostasis; Earth Systems Science; synthetic biology; terraformation

Online: 31 July 2023 (09:50:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0538.v1

Drainage Research of Different Tube Depth in Horizontal Gas Well

Xiuwu Wang, Wenmin Ma, Wei Luo, Ruiquan Liao

Subject: Engineering, Energy And Fuel Technology Keywords: horizontal gas well; liquid-carrying model; tubing depth; gas drainage; fluid energy

Online: 28 December 2022 (10:01:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints201611.0125.v1

Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype

Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe, Tamás Tereh, Nóra Kovács, Anita Hidasi, Barbara Bánkuti, Krisztina Buzás, Frederic Lemnitzer, Zsolt Ruzsics, Susan Szathmary, Hans Wolf, Dániel Salamon, János Minárovits, Hans-Helmut Niller

Subject: Biology And Life Sciences, Virology Keywords: Lamin, EBV latency, transformation, epigenetic regulation, activated B cell

Online: 24 November 2016 (17:53:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0481.v1

Genetic Characteristics and Pathogenicity for Partial Gene fragment of one Bovine Rotavirus in Yunnan Province, China

Chenxi Zhang, Zailei Li, Xinhui Duan, Hongya Yan, Xin Wu, Peiying Zhu, Wengui Li, Huafeng Gao

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Bovine rotavirus; Cattle diarrhea; Bioinformatic analysis

Online: 5 July 2024 (09:44:36 CEST)

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Group A rotaviruses (RVA) have been identified as the leading cause of neonatal calf diarrhea (NCD) In Yunnan, China, despite the limited documentation on this issue within the country. Given the significant impact on animal health and welfare, further attention is warranted to comprehend the prevalence and epidemiology of this disease. In this study, we isolated RVA strains from NCD clinical samples to support RVA epidemic study and further disease control in Yunnan. Material and Methods: Ten fecal samples were obtained from calves with a single episode of neonate calf diarrhea from a farmer during a severe NCD outbreak. For the diagnosis of Group A rotavirus (RVA) infection, fecal reverse transcription-polymerase chain reaction (RT-PCR) was employed as the primary method. Subsequently, RVA isolation was conducted on MA104 cells, with inoculates being pretreated with trypsin TPCK to enhance viral infectivity. The isolated RVA strain was further confirmed through RT-PCR and immunofluorescence assay. Results: RVA virus was isolated from clinical samples following six passages depended on trypsin treatment of inoculates. The sign of CPE detected consisted of increased cell granularity, obscure cell boundaries, cell rounding, and eventual degeneration and detachment of cells. Analysis of sequencing revealed that field isolates was of group A serotype. Phylogenetic analysis based on the ORF sequences of the VP4 and VP7 sequences segments indicated that the isolate was classified as the P[1] genotype. VP4 gene of strain YN-1 shares 75.2%-99.7% nucleotide identity of and 81.6%–99.7% amino acid identity with the complete VP4 sequences of the P[1]. The complete VP7 gene of YN-1 is 981 nucleotide long and encode 326 amino acids. The RotaC v2.0 analysis classified the sequence as the G8 genotype. The VP7 gene of strain YN-1 shares 88.5%-97.7% nucleotide identity and 94.2%-99.1% amino acid identity. Conclusion: This is the first study in China which reports the circulation of Group A NCD on cattle farms in the field. Our study constitutes a crucial and a necessary step allowing preventive and veterinary medicine to support RVA disease controls in Yunnan province, China.

Preprint REVIEW | doi:10.20944/preprints202404.1585.v1

One Health Scoping Review of the Epidemiology of Human Fascioliasis across Africa for the Period 2000–2022

Moses Adriko, Edridah M. Tukahebwa, Miph B. Musoke, David Muwanguzi, Stellah Nambuya, Patrick Vudriko, Kalinda Chester, Samson Mukaratirwa, Moses J. Chimbari, Anna-Sofie Stensgaard, Birgitte Vennervald, Lawrence Mugisha

Subject: Public Health And Healthcare, Other Keywords: human-fascioliasis; prevalence; epidemiology; Africa; One-Health

Online: 25 April 2024 (15:07:38 CEST)

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(1)Background: Fascioliasis is considered a neglected tropical disease affecting an estimated 17 million people in more than 70 countries worldwide. The highest prevalence of human fascioliasis is found in regions where livestock farming and consumption of raw or undercooked freshwater plants are common, such as parts of Africa, Asia, Europe, and Latin America. It is responsible for over 90,000 disability-adjusted years of life due to associated abdominal complications. We conducted a study to investigate the prevalence of human fascioliasis through a systematic review and meta-analysis of all available literature in Sun-Saharan Africa.(2). Methods: We screened a total of 126 publications using pubmed online search engine databases; Web of Science and CAB for articles published within a 20-year period between 2000 and 2022. We used a comprehensive search method using a combination of terms, parasite and disease names. The initial search resulted in a screening of articles by title, abstracts and full texts. The relevant information on the prevalence of human fascioliasis in Africa was extracted. Of the 126 publications, 33 articles were selected for meta-analysis. The essential information required was extracted from these documents and determined through statistical analysis in Microsoft Excel for calculating the prevalence of human fascioliasis in sub-Saharan Africa. The variance of each study was assessed using a bimodal distribution and heterogeneity assessment using a I2 index. Analysis was performed using random effects models.(3)Results: A total of 271 articles were searched through the database search and 33 articles were eligible studies that provided qualitative information on the prevalence of human fascioliasis in Africa. These make up 12.17% (n=33). The pooled prevalence estimates for human fascioliasis are 0.032% (IVhet PPE: 0.032% (95%CI 1.4-6.53). The highest prevalence rate was observed in Egypt with a prevalence of 100%. The prevalence of human fascioliasis is very high in some sub-Saharan countries, partly due to neglect of diagnostics, demonstrating the bias of available information for decision-making in human fascioliasis across Africa, given the spatial presence, scarcity and incompleteness are region.(4)Conclusions: The prevalence of Human fascioliasis is very high in some Sub-Saharan Countries partly due to neglect in diagnostics. This presents how highly focal with spatial occurrences, scarcity and patchy the available information for decision making is on Human fascioliasis across the Sub-Saharan African region.

Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202204.0104.v1

Statistical Modelling and Experimental Design for the Validation of Droplet Digital PCR Methods

Steffen Uhlig, Christopher Weidner, Bertrand Colson

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Method validation; droplet digital PCR; orthogonal factorial design; variance components; Poisson assumption; cloglog model; target DNA copies per droplet; Monte Carlo; prediction interval

Online: 12 April 2022 (08:46:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0279.v1

COVID19 PCR Testing and the Unprecedented Institutional Response in a Low-Resource Setting

Akintunde Orunmuyi, Olukemi Adekanmbi, Adeola Fowotade, Akindele Adebiyi, Adekunle Adeniji, Olukemi Amodu, E. Oluwabunmi Olapade-Olaopa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID19; sub-Sahara Africa; PCR testing; Capacity building

Online: 13 July 2020 (06:32:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1390.v1

Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

Mirko Sonntag, Vanessa K. Elgeti, Yevhen Vainshtein, Lucca Jenner, Jan Mueller, Thorsten Brenner, Sebastian O. Decker, Kai Sohn

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sepsis; antimicrobial resistances; cell-free DNA; precision diagnostics; Next-Generation sequencing; suppression PCR; real-time diagnostics; nanopore sequencing

Online: 22 April 2024 (10:13:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0526.v1

One Health Ecological Approach in Sustainable Wireless En-ergy Transfer aboard Electric Vehicles for Smart Cities

Adel Razek

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: One Health; Responsible Attitude; electric vehicles; wireless power transfer; smart cities; living tissues; biodiversity; clean energy

Online: 7 August 2024 (23:09:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202002.0099.v1

The Determination of HIV-1 RT Mutation Rate, Its Possible Allosteric Effects, and Its Implications on Drug Resistance

Joshua Yi Yeo, Ghin Ray Goh, Chinh Tran-To Su, Samuel Ken-En Gan

Subject: Biology And Life Sciences, Virology Keywords: retroviruses; HIV-1; reverse transcriptase; mutation rate; drug resistance, allostery

Online: 7 February 2020 (11:50:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints201711.0094.v1

Economic Analysis of Flat-Plate and U-Tube Solar Collectors Using an Al₂O₃ Nanofluid

Woobin Kang, Yunchan Shin, Honghyun Cho

Subject: Engineering, Mechanical Engineering Keywords: solar collector; nanofluid; thermal efficiency; economic analysis; CO2 reduction

Online: 15 November 2017 (04:44:54 CET)

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Preprint REVIEW | doi:10.20944/preprints201809.0385.v1

One Health, Fermented Foods and Gut Microbiota

Victoria Bell, Jorge Ferrão, Lígia Pimentel, Manuela Pintado, Tito Fernandes

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: One Health, fermented foods, microbiota, nutrition

Online: 19 September 2018 (13:50:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1505.v1

Diagnostic Accuracy of Multiplex PCR in Early Onset Neonatal Sepsis

Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: neonatal sepsis; early onset sepsis; multiplex PCR; infection; neonate; diagnosis; pathogen detection

Online: 24 October 2023 (08:11:56 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202403.1839.v1

The Path from Diagnosis to Surgical Treatment of Hemophilic Elbow Arthropaty Can Be a Risky One: A Case Report

Gianluigi Pasta, Salvatore Annunziata, Roberta Ruggieri, Dario Abruzzi, Paolo Arrigoni, Eugenio Jannelli, Francesco Benazzo, Luisella Pedrotti, Erika Maria Viola, Emérito Carlos Rodríguez-Merchán, Mario Mosconi

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: Hemophilia; elbow arthropathy; surgery; complications

Online: 29 March 2024 (10:03:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0559.v1

Optimization of RT-QuIC Assay Duration for Screening Chronic Wasting Disease in White-Tailed Deer

Gokhan Yilmaz, Tamara Morrill, Cian Ward, Gordon Mitchell, Andrei Soutyrine, Hanhong Dan, Min Lin, Jiewen Guan

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: RT-QuIC; Chronic Wasting Disease; Diagnostics; Optimization

Online: 7 September 2023 (15:45:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1249.v1

Flame Chemistry in the ISO/TS 19700 Steady State Tube Furnace

Gabrielle Peck, Ryan Hayes

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: Nitrogen; Smoke toxicity; Flame zone; Carbon monoxide; SSTF

Online: 20 November 2023 (14:05:21 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202309.0629.v1

Impedance Variation of a Coaxial Coil Encircling a Metal Tube Adapter

Yao Luo, Xinyi Yang

Subject: Physical Sciences, Applied Physics Keywords: Tube adapter; eddy current; numerical eigenfunctions; Clenshaw-Curtis quadrature

Online: 11 September 2023 (07:52:29 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202304.0581.v1

Piriform Fossa Injury during Calibration Tube Insertion in Laparoscopic Sleeve Gastrectomy

Taiki Nabekura, Takashi Oshiro, Kotaro Wakamatsu, Natsumi Kitahara, Yuki Moriyama, Kengo Kadoya, Ayami Sato, Tomoaki Kitahara, Tasuku Urita, Yu Sato, Masaru Tsuchiya, Shinich Okazumi

Subject: Medicine And Pharmacology, Surgery Keywords: laparoscopic sleeve gastrectomy; bariatric and metabolic surgery; complication; iatrogenic piriform fossa and esophageal injury; orogastric calibration tube

Online: 19 April 2023 (09:43:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0412.v1

Numerical Demonstration of In-Tube Liquid-Column Migration Driven by Photoisomerization

Kei Nitta, Takahiro Tsukahara

Subject: Engineering, Mechanical Engineering Keywords: computational fluid dynamics; contact line; droplet; lab-on-chip; liquid manipulation; microfluidics; non-invasive control; photochemical reaction; photoresponsible surfactant; two-phase flow; wettability

Online: 18 October 2018 (10:21:58 CEST)

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Preprint EDITORIAL | doi:10.20944/preprints201703.0012.v1

One Thousand Preprints Online

Subject: Social Sciences, Library And Information Sciences Keywords: preprints

Online: 1 March 2017 (17:46:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1505.v1

360o Fusion of the Lumbar Spine Using Flarehawk 9 : A Retrospective Study of 58 Patients Demonstrating Imagistic and Clinical Outcomes with One Year Follow-up

Konstantinos Zygogiannis, Ioannis Chatzikomninos, Savvas Moschos, Ioannis Palavos, Dimitrios Koulalis, Anastasios Kalampokis

Subject: Medicine And Pharmacology, Surgery Keywords: clinical outcomes; posterior lumbar fusion; posture alignment

Online: 26 March 2024 (08:26:33 CET)

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Preprint REVIEW | doi:10.20944/preprints202407.1270.v1

From the “One Molecule – One Target – One Disease” Concept towards Looking for Multi-target Therapeutics for Treating Non-polio Enterovirus (Npev) Infections

Hugo Roux, Franck Touret, Pascal Rathelot, Patrice Vanelle, Manon Roche

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Enterovirus; Multi-target directed ligand; Clinical Trials; Combination; Drug design

Online: 16 July 2024 (08:27:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Biology And Life Sciences, Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2272.v1

The Hypocholesterolemic Potential of the Edible Algae Fucus Vesiculosus: Proteomic and Quantitative PCR Analysis

Rebeca André, Rita Pacheco, Ana Catarina Alves, Hugo M. Santos, Mafalda Bourbon, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Fucus vesiculosus; cholesterol synthesis; cholesterol excretion; , Ezetimibe; , NPC1L1; ABCG5; qRT-PCR; mRNA

Online: 31 May 2023 (14:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0196.v1

SARS-CoV-2 PCR Persistence and Duration of COVID-19 Symptoms in Health Professionals

Tatiana Schäffer Gregianini, Ludmila Fiorenzano Baethgen, Richard Steiner Salvato, Ivone Andreatta Menegolla, CEVS COVID-19 Team, Ana Beatriz Gorini da Veiga

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: SARS-CoV-2; PCR persistent positive; respiratory infection; COVID-19

Online: 12 December 2022 (09:04:59 CET)

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Preprint ESSAY | doi:10.20944/preprints202404.1581.v1

Development and Experimental Study of an Experimental Setup for On-Line Vibrating Tube Liquid Densitometer

De fu Xu, Yao ning Sun, Wen lin Chen, Jun Mu, Peng Wang

Subject: Engineering, Other Keywords: online vibrating tube liquid densitometer; theoretical analysis; experimental setup; temperature; pressure

Online: 25 April 2024 (02:26:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201912.0207.v1

Experimental Generation of STKN540B Steel Circular Tube Manufacture with Press Bending and Auto-Welding Correction

Dongkyu Lee, Sungmin Joo, Ji Zhang

Subject: Engineering, Mechanical Engineering Keywords: press bending; orbital auto welding; steel-tube correction; STKN540B; high-strength steel tube; manufacturing process

Online: 16 December 2019 (06:20:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints201704.0148.v1

Multi-objective Optimization of Draft Tube in Francis Turbine Using DOE, RBF and NSGA-II

Chol Nam Mun, De Chun Ba, Xiang Ji Yue, Myong Il Kim

Subject: Engineering, Energy And Fuel Technology Keywords: francis turbine; draft tube; optimization design; experiment of design; non–dominated sorting genetic algorithm

Online: 24 April 2017 (10:52:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0600.v1

Real-Time Monitoring of SARS-CoV-2 Variants in Oklahoma Wastewater through Allele-Specific RT-qPCR

Kristen Shelton, Gargi N Deshpande, Gilson J Sanchez, Jason Vogel, A Caitlin Miller, Gabriel Florea, Erin R Jeffries, Kara B. De Leon, Bradley Stevenson, Katrin Gaardbo Kuhn

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: wastewater surveillance; SARS-CoV-2; variant; RT-qPCR; sequencing; early warning; demography; Oklahoma

Online: 9 September 2024 (08:42:34 CEST)

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