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Preprint ARTICLE | doi:10.20944/preprints202401.1468.v1

Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR

Claudio A. Henriquez, Natalia Riquelme, Alejandra Larach, Ximena Besoain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Virus-viroid detection; Mixed infections; Comparison of diagnostic methods; CTV; HSVd; Bioamplification

Online: 19 January 2024 (07:52:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0991.v1

Potential Performance of Two New RT-PCR and RT-q PCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes Allowing Efficient Entomological Surveillance of Both Vector-Borne Diseases

Michel Kiréopori Gomgnimbou, Louis robert wendyam BELEM, Etienne Bilgo, Miriam Félicité Amara, Zouera Laouali, Ali Ouari, Toussaint Bayala, Kobo Gnada, Raymond Kharlis Yao, Moussa Namountougou, Ibrahim Sangaré

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: multiplex rt-pcr; multiplex rt-qpcr; molecular detection; dengue virus; chikungunya virus; mosquitoes; Burkina Faso

Online: 12 July 2024 (08:43:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint REVIEW | doi:10.20944/preprints202405.1442.v1

Revalidation of DNA Fragmentation Analyses for Human Sperm—Measurement Principles, Comparative Standards, Calibration Curve, Required Sensitivity, and Eligibility Criteria for Test Sperm—A Step-by-Step Approach to Cohort Studies with Multivariable Analyses

Satoru Kaneko, Yuki Okada

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: DNA fragmentation; single-cell pulsed-field gel electrophoresis; human sperm; sperm chromatin structure assay; sperm chromatin dispersion test; comet assay; quantitative analysis

Online: 23 May 2024 (08:19:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0212.v1

One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3

Xueming Chen, Yuhuan Chen, Chunguo Liu, Xiaojun Li, Hongyu Liu, Xiaofei Bai, Ming Liu, Yun Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DHAV-1; DHAV-3; Phylogenetic analysis; One-tube RT-PCR; Simultaneously

Online: 22 January 2019 (11:08:19 CET)

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Working Paper BRIEF REPORT

Positive Rate Is Useful to Evaluate the Sufficiency of RT-PCR Test Availability for COVID-19

Sadao Suzuki

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; RT-PCR test; pre-test probability; positive rate

Online: 5 May 2020 (06:04:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1094.v1

Development and Evaluation of an In-House Real-Time Rt-Pcr Targeting nsp10 Gene for SARS-CoV-2 Detection

Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; nsp10; real-time RT-PCR; diagnostic

Online: 20 February 2024 (06:07:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Biology And Life Sciences, Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2272.v1

The Hypocholesterolemic Potential of the Edible Algae Fucus Vesiculosus: Proteomic and Quantitative PCR Analysis

Rebeca André, Rita Pacheco, Ana Catarina Alves, Hugo M. Santos, Mafalda Bourbon, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Fucus vesiculosus; cholesterol synthesis; cholesterol excretion; , Ezetimibe; , NPC1L1; ABCG5; qRT-PCR; mRNA

Online: 31 May 2023 (14:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201702.0011.v1

Simulation Analysis of Solution Transportation Absorption Chiller with the Capacity from 25 RT to 1000 RT

Koji Enoki, Fumi Watanabe, Seigo Tanaka, Atsushi Akiwawa, Toshitaka Takei

Subject: Engineering, Energy And Fuel Technology Keywords: absorption chiller; thermal energy transportation; solution transportation; ammonia-water; COP; simulation

Online: 4 February 2017 (07:49:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1366.v1

Cycle Threshold Values of SARS-CoV-2 RT-PCR During Outbreaks in Nursing Homes: A Retrospective Cohort Study.

Juan Carlos Gasco-Laborda, Maria Gil-Fortuño, Maria Dolores Tirado-Balaguer, Noemi Meseguer-Ferrer, Oihana Sabalza-Baztán, Óscar Pérez-Olaso, Iris Gómez-Alfaro, Sandrine Poujois-Gisbert, Noelia Hernández-Pérez, LLedó Lluch-Bacas, Viorica Rusen, Alberto Arnedo-Pena, Juan Bautista Bellido-Blasco

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: SARS-CoV-2; PCR; cycle threshold; infections; nursing homes; outbreaks; retrospective cohort

Online: 18 September 2024 (11:47:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1131.v1

Social Media Influencers: Customer Attitudes and Impact on Purchase Behaviour

Galina Ilieva, Tania Yankova, Margarita Ruseva, Stanislava Klisarova-Belcheva, Yulia Dzhabarova, Marin Bratkov

Subject: Business, Economics And Management, Marketing Keywords: social media marketing; influencer marketing; customer satisfaction; behaviour intention; purchase intention, structural equation modelling; PLS-SEM; Machine Learning; MCDM

Online: 17 May 2024 (15:51:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2501.v1

Assessment of Ukraineʹs Food Self‐Sufficiency against the background of either the European Union or Poland

Piotr Szajner, Iwona Szczepaniak, Wiesław Łopaciuk

Subject: Business, Economics And Management, Economics Keywords: food self‐sufficiency; food security; production potential of agriculture; war in Ukraine; European Union; Poland

Online: 31 July 2024 (11:45:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1460.v1

Three Cubes Packing for All Dimensions

Peter Adamko

Subject: Computer Science And Mathematics, Mathematics Keywords: packing of cubes; extreme

Online: 22 April 2024 (18:18:39 CEST)

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Preprint SHORT NOTE | doi:10.20944/preprints202306.1658.v1

Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry

Wenfa Ng

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: qPCR assay; probe-based detection; SYBR green; human KRAS gene; human gastric CloTest samples

Online: 23 June 2023 (10:50:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1226.v1

Digital PCR Validation for Characterization of Quantitative Reference Material of Escherichia coli O157:H7 Genomic DNA

Claudia Patricia Tere-Peña, Martha Nancy Calderon-Ozuna, John Emerson Leguizamón

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: E. coli O157:H7; Reference material; Method validation; digital PCR

Online: 18 June 2024 (11:03:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0440.v1

Clinical Performance Analysis and Evaluation of Quantitative Real Time PCR for Diagnosis of Scrub Typhus in North East India

Diptanu Paul, W Valarie Lyngdoh, Himesh Barman, Clarissa Jane Lyngdoh, Kryshanlang Lynrah, Elantamilan Durairaj

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Scrub typhus; qRT PCR; Quantitative PCR; real time PCR; IgM ELISA; North-East India

Online: 27 May 2020 (07:51:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201607.0021.v1

Solvency II Calibrations: Where Curiosity Meets Spuriosity

Stefan Mittnik

Subject: Business, Economics And Management, Finance Keywords: Solvency capital requirements capital, standard formula, near unit root, spurious correlation, VaR—implied correlations, tail dependence

Online: 12 July 2016 (09:38:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0313.v2

Heat-Induced Oxidative Stress and Use of Astaxanthin on the NF-kB, NFE2L2 Transcription Factors and Cytoprotective Capacity in the Thymus of Broilers

Donna Lee Kuehu, Yuanyuan Fu, Masaki Nasu, Hua Yang, Vedbar S. Khadka, Youping Deng

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: oxidative stress; astaxanthin; NF-kB; NFE2L2; cytoprotective capacity; thymus; broilers

Online: 26 June 2024 (06:30:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1127.v1

Machine Learning Application to Brain Diseases Detection

Aleksandar Simović, Maja Lutovac-Banduka, Kristina Davidović, Snežana Lekić

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; application; classification; brain diseases; detection

Online: 17 November 2023 (15:32:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0439.v1

SARS-CoV-2 Detection in Fecal Samples in Sym-asymptotic Patients with Typical Findings of COVID-19 on Ag-RDT and SARS-CoV-2 RT-PCR Tests

Richard Odongo Magwanga, Benson Onyango, Alfred Ochieng, Shehu Shagari Awandu, Pamela Were, Joy Nyangasi Kirungu, Marceline Adhiambo Oloo, Erick Muok, Stephen Munga, Fredrick Ogutu, Angeline Orwa, Fredrick Okumu, Clayton Onyango, Dorothy Awuor, Francis Orata, Justine Ochieng’-Midega, Bonface Oure, Regina Nyunja, Fredrick Ade, Sharley Indechi, Erick Okuto, Benson Estambale

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: COVID19; SARS-CoV-2 virus; Oro-nasopharyngeal; fecal; vaccination; asym-symptomatic; rapid diagnostic test

Online: 28 September 2022 (11:51:21 CEST)

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Coronavirus is a disease caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged as a global pandemic in 2019 from Wuhan, China. Since its emergence, it has caused immense suffering to human life, 6.27 million lives have been lost, movement curtailed and social dynamics disrupted. The golden standard for getting samples for SARS-CoV-2 detection is through oral- nasopharyngeal swab, this method of sample collection is invasive and uncomfortable, thus stigmatized the general population, and thereby impeded the progress of controlling the spread through mass testing. Being a contact disease, mechanisms to encourage mass testing is key to reduce the spread. This study thus developed a complimentary sample type to test for SARS-CoV-2, the use of human feces. Fecal samples were collected from 100 asym-symptomatic individuals suspected to be infected with COVID-19, virus RNA was then extracted and profiled through Real Time Polymerase Chain Reaction (RT-qPCR). The antigen rapid diagnostic test revealed high positivity rate of 44%, but the real time polymerase chain reaction results on nasopharyngeal and fecal samples revealed a significant variation, high number of the patients tested positive with stool samples compared to the nasopharyngeal swabs, with 43 and 37%, respectively. SARS-CoV-2 virus was detected in both symptomatic and asymptomatic individuals; however, the symptomatic registered a higher positivity of 25% compared to 20% among the asymptomatic patients. Vaccination only lowered the risk of infection, fully and partially vaccinated lowered the infection level to 10% compared to 20% among the unvaccinated. Finally, gender parity in relation to COVID19 was evaluated, more females (56%) compared to males were recruited in this study, out of which (20; 43.4%) were positive, and 26 (56.6%) were negative based on fecal RT-qPCR outcomes. Based on the outcome of this study, rapid diagnostic test (Ag-RDT) however cheap and or fast does not provide accurate information, moreover, the virus does not stay longer within the Oro-nasopharyngeal region, thus the invalid or negative results, thus use of feces should be adopted as a confirmatory test to ascertain the COVID19 status of an individual.

Preprint ARTICLE | doi:10.20944/preprints202407.0037.v1

Evaluation of in Vitro Serotonin-Induced Electrochemical Fouling Performance of Boron Doped Diamond Microelectrode using Fast-Scan Cyclic Voltammetry

Mason L. Perillo, Bhavna Gupta, James R. Siegenthaler, Isabelle E. Christensen, Brandon Kepros, Abu Mitul, Ming Han, Robert Rechenberg, Michael F. Becker, Wen Li, Erin K. Purcell

Subject: Engineering, Bioengineering Keywords: Serotonin, Fast Scan Cyclic Voltammetry, Boron Doped Diamond, Sensing, Neuroscience, Biomedical Engineering, Dopamine, Electrochemical Fouling, Neurotransmitter Detection, Microelectrodes

Online: 1 July 2024 (09:20:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0387.v1

Acquisition of Humoral Immune Responses in Convalescent Japanese People with SARS-CoV-2 (COVID-19) Infection in 2021

Koshiro Monzen, Takanori Watanabe, Toshihiro Okabe, Hisakuni Sekino, Hironori Nakagami, Ryuichi Morishita

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2; Convalescent serum; Neutralizing antibody

Online: 4 August 2023 (12:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201809.0413.v1

Comparison of Quantitative PCR (qPCR) Paenibacillus larvae Targeted Assays and Definition of Optimal Conditions for Its Detection/Quantification in Honey and Hive Debris

Franca Rossi, Carmela Amadoro, Addolorato Ruberto, Luciano Ricchiuti

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Paenibacillus larvae; optimized qPCR; quantification; honey; hive debris

Online: 20 September 2018 (14:13:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1381.v1

Results on Cumulative Entropic Properties of Consecutive Systems

Mashael A. Alshehri, Mohamed Kayid

Subject: Engineering, Control And Systems Engineering Keywords: Consecutive k-out-of-n:F systems; Cumulative entropy; Shannon entropy; Stochas- tic orders; Real data

Online: 20 August 2024 (03:40:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.2032.v1

Use of Microalgae-Derived Astaxanthin to Improve Cytoprotective Capacity in the Ileum of Heat-Induced Oxidative Stressed Broilers

Donna Lee Kuehu, Yuanyuan Fu, Masaki Nasu, Hua Yang, Vedbar S. Khadka, Youping Deng

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: astaxanthin; cytoprotective capacity; ileum; heat stress; oxidative stress; broilers

Online: 30 May 2024 (13:13:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0446.v1

Symbolic Analysis of Classical Neural Networks for Deep Learning

Maja Lutovac-Banduka, Igor Franc, Vladimir Milićević, Nemanja Zdravković, Nikola Dimitrijević

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; visual programming language; closed-form expression; theoretical justification; feature extraction; complexity; optimization and validation

Online: 7 November 2023 (11:25:29 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.0062.v1

A Survey of Quantitative Techniques in Electricity Consumption – A Global Perspective

Atif Maqbool Khan, Artur Wyrwa

Subject: Business, Economics And Management, Econometrics And Statistics Keywords: Survey; Quantitative Methods; Energy Consumption

Online: 1 August 2024 (11:55:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0030.v1

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi, Hussein Alburkat, Taisir Abdulelah Kadhim, Ammar M S Al-Helli, Mustafa Hamza Ghazi, Yasir Abdulameer N. Aldabbagh, Reem Numaan Talib, Sama Hussein Ali, Noor Hameed Imran, Ruaa Almohsen, Turki Muftin Saad, Pallavi Mandave

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: PCR; Salmonella Typhi; Widal Test; BHI

Online: 1 November 2022 (12:43:58 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1089.v1

White Paper Textile Fibre Recycling Technologies

Birgit Stubbe, Stijn Van Vrekhem, Sofie Huysman, Rémi G. Tilkin, Isabel De Schrijver, Myriam Vanneste

Subject: Chemistry And Materials Science, Paper, Wood And Textiles Keywords: textile recycling; fibre-to-fibre recycling; textile sorting; textile disassembly; digital textile platforms

Online: 17 November 2023 (08:49:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints201612.0038.v1

Chemical Fingerprint Analysis and Quantitative Analysis of Rosa Rugosa by UPLC-DAD

Sanawar Mansur, Rahima Abdullab, Amatjan Ayubicb, Haji Akbar Aisa

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Rosa rugosa; UPLC-DAD; quality control; fingerprint

Online: 7 December 2016 (11:11:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.1783.v1

Quantitative Trait Loci Mapping and Candidate Gene Analysis for Fiber Quality Traits in Upland Cotton

Xiaoyun Jia, Hongxia Zhao, Jijie Zhu, Hantao Wang, Shijie Wang, Miao Li, Guoyin Wang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: upland cotton; fiber quality; QTL mapping; candidate gene; qRT-PCR

Online: 25 June 2024 (15:12:46 CEST)

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Superior fiber quality is one of the most important objectives in cotton breeding. To detect the genetic basis underlying fiber quality, an F2 population containing 413 plants was constructed by crossing Jifeng 914 and Jifeng 173, both of which have superior fiber quality, with Jifeng 173 being better. Five fiber quality traits were investigated in the F2, F2:3, F2:4, and F2:5 populations. Quantitative trait loci (QTL) mapping was conducted based on a high-density genetic map containing 11,488 single nucleotide polymorphisms (SNPs) and spanning 4,202.12 cM in length. Transgressive segregation patterns and complex correlations in the five tested traits were observed. A total of 108 QTLs were found, including 27 for fiber length (FL), 16 for fiber strength (FS), 24 for micronaire (MC), 17 for fiber uniformity (FU), and 24 for fiber elongation rate (FE). Chromosome A7 contained 12 QTL, ranking the first. No QTL was found on chromosome D1 and D11. Each QTL contributed 1.98%–21.45% to the phenotypic variation (PV), including 13 major effect QTLs that contributed more than 10% toward PV. Two QTLs could be repeatedly detected in three populations, including qFL-D3-2 in F2, F2:4, and F2:5 with 9.18%–21.45% of PV, qFS-A11-1 in F2:3, F2:4 and F2:5 with 6.05%–10.41% of PV. Another seven stable QTLs could be detected in two populations, including four major effect QTLs: qFL-A12-3, qFS-D10-2, qMC-D6-2, and qMC-D8-1. Fourteen QTL-overlapping regions were found, which might explain the complex correlations among the five phenotypic traits. Four regions on chromosome A11, D3, D6, and D10 covered by both stable and major effect QTLs are promising for further fine mapping. The genomic regions of the two QTLs detected in three populations and the four major effect QTLs contain 810 genes. Gene functional analysis revealed that the annotated genes are mainly involved in protein binding and metabolic pathways. Fifteen candidate genes in the qFL-D3-2 region are highly expressed in fiber or ovules during fiber initiation, elongation, secondary cell wall thickening, or maturation stages. qRT-PCR revealed that Ghir_D03G005440.1 and Ghir_D03G011310.1 may play a role in promoting fiber initiation, Ghir_D03G006470.1 may be beneficial for promoting fiber elongation. This study provides more information for revealing the molecular genetic basis underlying cotton fiber quality.

Preprint ARTICLE | doi:10.20944/preprints202407.1957.v1

Quantitative Assessment of Drone Pilot Performance

Daniela Doroftei, Geert De Cubber, Salvatore Lo Bue, Hans De Smet

Subject: Computer Science And Mathematics, Robotics Keywords: Performance assessment; Pilot training; Human performance modelling; Quantitative validation; Standardised tests; Drone simulator; AI copilot

Online: 24 July 2024 (17:48:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201612.0147.v1

A MEMS Device for Quantitative in situ Mechanical Testing in Electron Microscope

Xiaodong Wang, Shengcheng Mao, Jianfei Zhang, Zhipeng Li, Qingsong Deng, Jin Ning, Xudong Yang, Li Wang, Yuan Ji, Xiaochen Li, Yinong Liu, Ze Zhang, Xiaodong Han

Subject: Chemistry And Materials Science, Nanotechnology Keywords: piezoresistive sensor; electron microscope; in situ mechanical test

Online: 30 December 2016 (04:16:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1995.v1

Analysis and Optimization of Quantitative Casting Motion of a Casting and Pouring Robot

Biaoxiong Xie, Xingang Miao

Subject: Engineering, Mechanical Engineering Keywords: casting robot, fifth degree polynomial interpolation, motion force analysis, PID algorithm

Online: 30 April 2024 (11:43:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0834.v1

Forging the Iron-Net: Towards a Quantitative Understanding of Microbial Communities via Siderophore-Mediated Interactions

Shaohua Gu, Ruolin He, Guanyue Xiong, Zeyang Qu, Yuanzhe Shao, Linlong Yu, Di Zhang, Fanhao Wang, Ruichen Xu, Peng Guo, Ningbo Xi, Yinxiang Li, Yanzhao Wu, Zhong Wei, Zhiyuan Li

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: iron-net; siderophores; microbial community; ecology; evolution

Online: 11 July 2024 (09:59:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0884.v1

Prediction of Dielectric Constant in Series of Polymers by Quantitative Structure-Property Relationship (QSPR)

Estefania Ascencio Medina, Shan He, Amirreza Daghighi, Kweeni Iduoku, Gerardo M. Casanola-Martin, Sonia Arrasate, Humerto Gonzalez-Diaz, Bakhtiyor Rasulev

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: dielectric constant; polymers; QSPR; Gradient Boosting Regressor; Accumulated Local Effect

Online: 13 August 2024 (08:55:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1398.v1

Research on Travel Route Planning Optimization based on Large Language Model

Bohang Li, Kai Zhang, Yiping Sun, Jianke Zou

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: travel route planning; large language model; graph search algorithm; route optimization

Online: 17 July 2024 (09:41:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0262.v1

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Ju Huang, Ligong Zhai, Junyin Wang, Xiaotian Sun, Baoshi Wang, Zhaohui Wei

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Pseudomonas aeruginosa; Pseudomonas fragi; simultaneous detection; droplet digital PCR

Online: 3 April 2024 (16:17:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0604.v2

RT-qPCR Diagnostics: The “Drosten” Sars-Cov-2 Assay Paradigm

Stephen Bustin, Sara Kirvell, Jim F Huggett, Tania Nolan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID-19; reverse transcription; qPCR; SARS-CoV-2; molecular diagnosis

Online: 3 August 2021 (15:34:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201608.0084.v2

Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry

Xiaowei Fang, Shuiping Yang, Konstantin Chingin, Liang Zhu, Xinglei Zhang, Zhiquan Zhou, Zhanfeng Zhao

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: extractive electrospray ionization; rapid detection; malachite green; water; mass spectrometry

Online: 11 August 2016 (10:00:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0148.v1

Quantitative Analysis of Urban Microplastic Dissemination and Accumulation in Marine Ecosystems: Pathways, Processes, and Impacts

Areej Alqarni

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Microplastic Pollution; Urban Sources; Marine Ecosystems; Environmental Impact; Waste Management; Riverine Transport; Polymer Types; Seasonal Variations; Ecological Consequences; Mitigation Strategies

Online: 2 April 2024 (15:38:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0658.v1

Quantitative and Qualitative Analysis of Main Effects and Interaction Parameters in Thermoacoustic Refrigerators Performance

Humberto Peredo Fuentes, Carlos Amir Escalante Velazquez

Subject: Physical Sciences, Applied Physics Keywords: Thermoacoustics; Design of experiments; Linear Thermoacoustic Theory; Pearson Analysis; ANOVA; Transfer Function

Online: 9 September 2024 (11:48:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0299.v1

Optimizing Droplet Digital PCR for Environmental Samples

Vasilis Kokkoris, Eric Vukicevich, Andrew Richards, Corrina Thomsen, Miranda Hart

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ddPCR, droplet digital PCR, environmental samples, environmental DNA, soil microbiology

Online: 12 April 2021 (12:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0203.v1

Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken

Subject: Biology And Life Sciences, Insect Science Keywords: Tungiasis; Tunga penetrans; molecular entomology; DNA isolation; Phusion® polymerase; FIREpol® Taq polymerase; low-cost PCR,

Online: 10 November 2022 (11:19:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0253.v1

Statistical Signatures of Abstraction in Deep Neural Networks

Carlo Orientale Caputo, Matteo Marsili

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Machine Learning; Statistical Physics; learning theory

Online: 3 July 2024 (05:32:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0698.v1

A Comparative Study of Susceptibility and Hazard for Mass Movements Applying Quantitative Machine Learning Techniques. Case Study: Northern Lima Commonwealth, Peru

Edwin Noe Badillo-Rivera, Manuel Daniel Olcese Huerta, Ramiro Santiago Chirinos, Teófilo Ivan Poma Inche, Neftalí Euclides Muñoz Fernandez, Carlos Alberto Rojas-León, Teodosio Juan Chávez Campos, Luz de Fátima Eyzaguirre Gorvenia, César Augusto Rodriguez Aburto, Fernando José Oyanguren Ramírez

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: mass movement, weight evidence, principal component analysis, machine learning.

Online: 10 May 2024 (14:15:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202004.0216.v1

Development of a Field-deployable RT-qPCR Workflow for COVID-19 Detection

Raphael Nyaruaba, Bo Zhang, Caroline Muema, Elishiba Muturi, Greater Oyejobi, Jin Xiong, Bei Li, Zhengli Shi, Caroline Mwaliko, Junping Yu, Xiaohong Li, Hongping Wei

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: COVID-19; SARS-CoV-2; field work; community; diagnosis; rapid detection; inactivation; RT-qPCR

Online: 14 April 2020 (08:41:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1633.v1

A hybrid Fuzzy Evaluation Method for Quantitative Risk Classification of Barrier Lake Based on an AHP Method Extended by D Numbers

Qigui Yang, Fugen Yan, Yaojun Cai, Yuesheng Luan, Duliangzi Yi, Haitao Liu, Wanli Dai, Zhongtian Zou

Subject: Engineering, Civil Engineering Keywords: barrier lake; risk classification; weight; D-AHP; short window; information acquisition

Online: 22 August 2024 (14:47:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1182.v1

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Laura Sancha Domínguez, Ana Cotos Suárez, María Sánchez Ledesma, Juan Luis Muñoz Bellido

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: dPCR; digital PCR; infection; bacteria; virus; parasites

Online: 20 March 2024 (08:24:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1328.v1

Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of Bacteroides fragilis

Bakhtiyar Mahmood, Anna Paunkov, Malgorzata Kupc, Katalin Burián, Erzsébet Nagy, David Leitsch, József Sóki

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Bacteroides, metronidazole, nim, resistance mechanism, RT-qPCR

Online: 17 January 2024 (13:06:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202102.0525.v1

Exploring New Scaffolds for the Dual Inhibition of HIV-1 Rt Polymerase and Ribonuclease Associated Functions

Rita Meleddu, Angela Corona, Simona Distinto, Filippo Cottiglia, Serenella Deplano, Lisa Sequeira, Daniela Secci, Alessia Onali, Erica Sanna, Francesca Esposito, Italo Cirone, Francesco Ortuso, Stefano Alcaro, Enzo Tramontano, Peter Matyus, Elias Maccioni

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: HIV-RT; ribonuclease H; dual inhibitors; docking; putative binding

Online: 23 February 2021 (15:51:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1230.v1

Quantitative Assessment and Analysis of Fish Behavior in Closed Systems Using Information Entropy

Minoru Kadota, Shinsuke Torisawa, Tsutomu Takagi

Subject: Biology And Life Sciences, Behavioral Sciences Keywords: Information theory; fish behavior; entropy; quantitative assessment; random walk model

Online: 20 May 2024 (12:37:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1746.v1

Quantitative approach to quality review of prenatal ultrasound examinations: Fetal biometry

C. Andrew Combs, MD, PhD, Sushma Amara, MD, Carolyn Kline MD, Olaide Ashimi Balogun, MD, Zachary Bowman, MD, PhD

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: abdominal circumference, femur length, head circumference, image review, quality review, variation, z-score

Online: 25 June 2024 (08:46:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1746.v1

Perception of Selected Factors on the Sustainability of SMEs: Empirical Study from Visegrad Group Countries

Mária Hudáková, Juraj Cúg, Peter Karácsony, Zora Petráková, Ján Dvorský, Michał Bernard Pietrzak

Subject: Business, Economics And Management, Business And Management Keywords: business risk; case study; questionnaire; SMEs; sustainability; Visegrad Group countries.

Online: 29 February 2024 (13:50:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1390.v1

Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection

Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: droplet Digital PCR; canine distemper virus; molecular diagnosis

Online: 18 September 2024 (10:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1177.v1

A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis

Thaís Ellen Chaves Gomes, Victor Coutinho Bastos, Douglas Boniek, Mário Romañach, Fernanda Faria Rocha, Roberta Rayra Martins Chaves, Ricardo Santiago Gomez

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Aspergillosis; Aspergillus; PCR; Diagnosis

Online: 15 August 2024 (14:30:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0326.v1

Molecular Characterization of Termitomyces Protoclones through PCR and RFLP Based DNA Typing

Pijush Mallick, Sobhan Roy Chowdhury, Dipanwita Roy, Subhash Kanti Roy, Samir Ranjan Sikdar

Subject: Biology And Life Sciences, Horticulture Keywords: DNA markers; edible mushroom; genome stability; protoplast regeneration

Online: 25 April 2018 (08:26:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202012.0728.v1

Partition Quantitative Assessment (Pqa): A Quantitative Methodology to Assess the Embedded Noise in Clustered Omics and Systems Biology Data

Diego A. Camacho-Hernández, Victor E. Nieto-Caballero, José E. León-Burguete, Julio A. Freyre-González

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: omics data; hierarchical clustering; noise quantification

Online: 29 December 2020 (14:02:28 CET)

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Preprint REVIEW | doi:10.20944/preprints201608.0160.v2

Cranberries and Cancer: An Update of Preclinical Studies Evaluating the Cancer Inhibitory Potential of Cranberry and Cranberry Derived Constituents

Katherine M. Weh, Jennifer Clarke, Laura A. Kresty

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: cranberry; cancer; proanthocyanidin; quercetin; ursolic acid

Online: 18 August 2016 (07:44:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1049.v1

Pattern Formation in a Model of Inflammation

Wissam El Hajj, Maxim Kuznetsov, Vitaly Volpert

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: inflammation; modelling; pattern formation; Turing structures

Online: 17 June 2024 (08:35:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201611.0125.v1

Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype

Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe, Tamás Tereh, Nóra Kovács, Anita Hidasi, Barbara Bánkuti, Krisztina Buzás, Frederic Lemnitzer, Zsolt Ruzsics, Susan Szathmary, Hans Wolf, Dániel Salamon, János Minárovits, Hans-Helmut Niller

Subject: Biology And Life Sciences, Virology Keywords: Lamin, EBV latency, transformation, epigenetic regulation, activated B cell

Online: 24 November 2016 (17:53:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0192.v1

Correlating Quantitative and Genomic SARS-CoV-2 Wastewater Data with Clinical Metrics in Metropolitan Perth, Western Australia

Jake Gazeley, Terence Lee, Daniel R Knight, Alexander Shivarev, Cameron Gordon, David Speers, Dylan D Barth, Jelena Maticevic, Meredith Hodge, Paul Armstrong, Paul Knight, Sandra Sjollema, Avram Levy

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Wastewater-based epidemiology; SARS-CoV-2; COVID-19; surveillance system; correlation analysis; genomics

Online: 5 March 2024 (09:07:35 CET)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202204.0104.v1

Statistical Modelling and Experimental Design for the Validation of Droplet Digital PCR Methods

Steffen Uhlig, Christopher Weidner, Bertrand Colson

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Method validation; droplet digital PCR; orthogonal factorial design; variance components; Poisson assumption; cloglog model; target DNA copies per droplet; Monte Carlo; prediction interval

Online: 12 April 2022 (08:46:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0279.v1

COVID19 PCR Testing and the Unprecedented Institutional Response in a Low-Resource Setting

Akintunde Orunmuyi, Olukemi Adekanmbi, Adeola Fowotade, Akindele Adebiyi, Adekunle Adeniji, Olukemi Amodu, E. Oluwabunmi Olapade-Olaopa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID19; sub-Sahara Africa; PCR testing; Capacity building

Online: 13 July 2020 (06:32:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1390.v1

Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

Mirko Sonntag, Vanessa K. Elgeti, Yevhen Vainshtein, Lucca Jenner, Jan Mueller, Thorsten Brenner, Sebastian O. Decker, Kai Sohn

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sepsis; antimicrobial resistances; cell-free DNA; precision diagnostics; Next-Generation sequencing; suppression PCR; real-time diagnostics; nanopore sequencing

Online: 22 April 2024 (10:13:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202102.0519.v1

Definitions and Concepts for Quantitative Rockfall Hazard and Risk Analysis

Didier Hantz, Jordi Corominas, Giovanni B. Crosta, Michel Jaboyedoff

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: rockfall; failure; propagation; hazard; risk; probability; frequency

Online: 23 February 2021 (14:23:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202101.0579.v2

Interpreting and Comparing Convolutional Neural Networks: A Quantitative Approach

Mohammad Mohaiminul Islam, Zahid Hassan Tushar

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Network Interpretation; Image Classification; Convolutional Neural Network; Integrated Gradient

Online: 22 November 2021 (14:06:52 CET)

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Preprint REVIEW | doi:10.20944/preprints202002.0099.v1

The Determination of HIV-1 RT Mutation Rate, Its Possible Allosteric Effects, and Its Implications on Drug Resistance

Joshua Yi Yeo, Ghin Ray Goh, Chinh Tran-To Su, Samuel Ken-En Gan

Subject: Biology And Life Sciences, Virology Keywords: retroviruses; HIV-1; reverse transcriptase; mutation rate; drug resistance, allostery

Online: 7 February 2020 (11:50:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1505.v1

Diagnostic Accuracy of Multiplex PCR in Early Onset Neonatal Sepsis

Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: neonatal sepsis; early onset sepsis; multiplex PCR; infection; neonate; diagnosis; pathogen detection

Online: 24 October 2023 (08:11:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0559.v1

Optimization of RT-QuIC Assay Duration for Screening Chronic Wasting Disease in White-Tailed Deer

Gokhan Yilmaz, Tamara Morrill, Cian Ward, Gordon Mitchell, Andrei Soutyrine, Hanhong Dan, Min Lin, Jiewen Guan

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: RT-QuIC; Chronic Wasting Disease; Diagnostics; Optimization

Online: 7 September 2023 (15:45:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202107.0307.v1

Quantitative Approaches to Study Retinal Neurogenesis

Diego Perez-Dones, Mario Ledesma-Terron, David G Míguez

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Retinogenesis; Quantiative Biology; Imaging

Online: 13 July 2021 (12:18:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0196.v1

SARS-CoV-2 PCR Persistence and Duration of COVID-19 Symptoms in Health Professionals

Tatiana Schäffer Gregianini, Ludmila Fiorenzano Baethgen, Richard Steiner Salvato, Ivone Andreatta Menegolla, CEVS COVID-19 Team, Ana Beatriz Gorini da Veiga

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: SARS-CoV-2; PCR persistent positive; respiratory infection; COVID-19

Online: 12 December 2022 (09:04:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1742.v1

Quantitative Assessment and Impact Analysis of Land Surface Deformation in Wuxi Based on PS-InSAR and GARCH Model

Shengyi Zhang, Lichang Xu, Rujian Long, Le Chen, Shenghan Wang, Shaowei Ning, Fan Song, Linlin Zhang

Subject: Environmental And Earth Sciences, Environmental Science Keywords: PS-InSAR; Wuxi city; Surface volatility; Sentinel-1A; Groundwater level

Online: 29 March 2024 (05:28:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0358.v1

Proposed Standard Test Protocols and Outcome Measures for Quantitative Comparison of Emissions from Electronic Nicotine Delivery Systems

Edward C. Hensel, Nathan C. Eddingsaas, Qutaiba M. Saleh, S. Jayasekera, S. Emma Sarles, A. Gary DiFrancesco, and Risa J. Robinson

Subject: Engineering, Automotive Engineering Keywords: ecigarette; regulatory science; substantial equivalence; public health; tobacco product comparison.

Online: 22 December 2021 (12:04:52 CET)

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This study introduces and demonstrates a comprehensive, accurate, unbiased approach to robust quantitative comparison of Electronic Nicotine Delivery Systems (ENDS) appropriate for establishing substantial equivalence (or lack thereof) between tobacco products. The approach is demonstrated across a family of thirteen pen- and pod-style ENDS products. Methods employed consist of formulating a robust emissions surface regression model, quantifying the empirical accuracy of the model as applied to each product, evaluating relationships between product design characteristics and maximum emissions characteristics, and presenting results in formats useful to researchers, regulators, and consumers. Results provide a response surface to characterize emissions (total particulate matter and constituents thereof) from each ENDS appropriate for use in a computer model and for conducting quantitative exposure comparisons between products. Results demonstrate that emissions vary as a function of puff duration, flow rate, E-Liquid composition, and device operating power. Further, results indicate that regulating design characteristics of ENDS devices and consumables may not achieve desired public health outcomes; it is more effective to regulate maximum permissible emissions directly. Three emissions outcome measures (yield per puff, mass concentration and constituent mass ratio) are recommended for adoption as standard quantities for reporting by manufacturers and research laboratories. The approach provides a means of (a) quantifying and comparing maximal emissions from ENDS products spanning their entire operating envelope, (b) comparative evaluation of ENDS devices and consumable design characteristics, and (c) establishing comparative equivalence of maximal emissions from ENDS. A consumer-oriented product emissions dashboard is proposed for comparative evaluation of ENDS exposure potential. Maximum achievable power dissipated in the coil of ENDS is identified as a potentially effective regulatory parameter.

Preprint ARTICLE | doi:10.20944/preprints202403.0507.v1

Parents’ Participation in Care during Neonatal Intensive Care Unit Stay in COVID-19 Era: A Quantitative Observational Study

Emanuele Buccione, Davide Scarponcini Fornaro, Damiana Pieragostino, Luca Natale, Adelaide D'Errico, Valentina Chiavaroli, Laura Rasero, Stefano Bambi, Carlo Della Pelle, Susanna Di Valerio

Subject: Public Health And Healthcare, Nursing Keywords: neonatal intensive care unit; parenthood; newborns; preterm; nurses

Online: 8 March 2024 (11:04:21 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.0040.v1

Progress and Prospect of Quantitative Research on Divination in Yijing

Xinqi Zheng, Haiyan Liu

Subject: Computer Science And Mathematics, Computational Mathematics Keywords: Yijing; overall quantization; five-element quantization; mixed quantization

Online: 2 October 2023 (04:33:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202011.0569.v1

Quantitative Imaging and Radiomics in Multiple Myeloma: opportunity or hype?

Alberto Stefano Tagliafico, Alida Dominietto, Liliana Belgioia, Cristina Campi, Daniela Schenone, Michele Piana

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: multiple myeloma; computed tomography; artificial intelligence; radiomics; prognosis; imaging; diagnosis

Online: 23 November 2020 (09:17:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0600.v1

Real-Time Monitoring of SARS-CoV-2 Variants in Oklahoma Wastewater through Allele-Specific RT-qPCR

Kristen Shelton, Gargi N Deshpande, Gilson J Sanchez, Jason Vogel, A Caitlin Miller, Gabriel Florea, Erin R Jeffries, Kara B. De Leon, Bradley Stevenson, Katrin Gaardbo Kuhn

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: wastewater surveillance; SARS-CoV-2; variant; RT-qPCR; sequencing; early warning; demography; Oklahoma

Online: 9 September 2024 (08:42:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0581.v1

Looking into the Quantification of Forensic Samples with Real-Time PCR

Ugo Ricci, Dario Ciappi, Ilaria Carboni, Claudia Centrone, Irene Giotti, Martina Petti, Alice Brogi, Elisabetta Pelo

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: DNA amplification; polymerase chain reaction; DNA quantification; forensic DNA

Online: 9 May 2024 (10:39:14 CEST)

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The quantification of human DNA extracts from forensic samples plays a key role in the forensic genetics process. Quantification guarantees maximum efficiency and avoids repeated analyses, over amplified samples, or unnecessary examinations. Here, we present our experience using an extremely sensitive real-time-based method with the Quantifiler® Trio DNA quantification kit, which we have explored in the ISO/IEC17025 accredited test validation process conducted in our laboratory. In this study, our focus was on establishing a minimum threshold beyond which DNA typing is unnecessary, by defining the correlation of negative quantification results with the absence of genetic profiles resulting from DNA typing. We investigated the results in the range in which the technique is used (50-0.005 ng/μl). In the validation process of the internally accredited method, we used data from the commercial quantification system, based on the average values of the standard curve using a wide range of samples, including artificially degraded samples. This method was extensively used continuously for nine years to quantify the DNA extract coming from a wide range of samples from criminal cases. The validation allowed us to verify the limits of the technique, especially in the use of the minimum quantities necessary to obtain genetic profiles acceptable for sending to the Italian DNA database. This optimization of the protocol for analysing forensic samples allows us to optimise the protocol for analysing forensic samples. However, the technique is used in our laboratory with a completely manual preparative phase, and we have decided to investigate the contribution of human error more accurately. Thus, in our experience, we have found that it is preferable to use quantification ranges rather than exact limits before deciding how to analyse the extracts via PCR. In addition, here we present some preliminary data related to the analysis of certain samples that initially appeared negative during quantification, applying a concentration method for maximum yield from forensic samples of judicial interest. Our aim is to ensure that no source of evidence is lost in the reconstruction of a criminal event.

Preprint ARTICLE | doi:10.20944/preprints202111.0210.v1

Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System

Shang Wang, Ying Tang, Jiehong Wei, Shan Wang, Guoyu Peng, Yuqing Li, Song Wu

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: CRISPR-Cas, Nucleic acid detection, Klebsiella pneumonia

Online: 11 November 2021 (12:57:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0189.v1

Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification

Lorena G. Caligiuri, Adolfo E. Sandoval, Jose C. Miranda, Felipe A. Pessoa, María S. Santini, Oscar D. Salomón, Nagila F. C. Secundino, Christina McCarthy

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sand fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia

Online: 17 December 2018 (10:41:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2063.v1

Multi-Echo Complex Quantitative Susceptibility Mapping and Quantitative Blood Oxygen Level Dependent Magnitude (mcQSM+qBOLD or mcQQ) for Oxygen Extraction Fraction (OEF) Mapping

Junghun Cho, Jinwei Zhang, Pascal Spincemaille, Hang Zhang, Thanh D. Nguyen, Shun Zhang, Ajay Gupta, Yi Wang

Subject: Engineering, Bioengineering Keywords: oxygen extraction faction; quantitative susceptibility mapping; quantitative blood oxygen level-dependent imaging; multi-echo complex quantitative susceptibility mapping and quantitative blood oxygen level dependent magnitude; QSM+qBOLD; QQ; mcQQ; deep learning; magnetic resonance imaging; MRI

Online: 27 December 2023 (09:10:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.0885.v1

TAS2R38 Genotype Does Not Affect SARS-CoV-2 Infection in Primary Ciliary Dyskinesia

Gioia Piatti, Giorgia Girotto, Maria Pina Concas, Leonardo Braga, Umberto Ambrosetti, Mirko Aldè

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: bitter taste receptors; TAS2R38; Primary Ciliary Dyskinesia; COVID-19; SARS-CoV-2

Online: 13 June 2024 (05:37:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1567.v1

Spatiotemporal Evolution and Tapio Decoupling Analysis of Energy-Related Carbon Emissions Using Nighttime Light Data: A Quantitative Case Study at the City Scale in Northeast China

Bin Liu, Jiehua Lv

Subject: Social Sciences, Urban Studies And Planning Keywords: energy carbon emissions; nighttime light (NTL) data; spatiotemporal evolution; Tapio decoupling analysis; Northeast China

Online: 21 August 2024 (16:58:03 CEST)

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As the world’s second-largest economy, China has undergone rapid industrialization and urbanization, leading to high energy consumption and significant carbon emissions. This development has exacerbated conflicts between human-land relations and environmental conservation, contributing to global warming and urban air pollution, both of which pose serious health risks. This study utilizes nighttime light (NTL) data from 2005 to 2019, combined with scaling techniques and statistical analysis, to estimate city-scale energy carbon emissions over a 15-year period. Our focus is on Northeast China, a traditional industrial region comprising 36 cities across three provinces, rich in natural resources but now facing severe aging challenges. We analyzed the spatial patterns of energy carbon emissions, while spatiotemporal evolution was assessed through spatial autocorrelation and dynamic changes. These dynamic changes were further evaluated using standard deviation ellipse (SDE) parameters and SLOPE values. Additionally, we examined the conflict between city-scale emissions and economic growth using the Tapio decoupling index. Our findings for the 36 cities over the 15-year period include: (1) Heilongjiang shows low emissions with a downward trend; Jilin shows overall improvement; Liaoning has high and steadily increasing emissions. (2) The global spatial autocorrelation of energy carbon emissions is significant, with a positive Moran’s I, while significant local Moran’s I clusters are concentrated in Heilongjiang and Liaoning. (3) The SDE shows the greatest changes in city-scale emissions occurred in 2015, followed by 2019, 2005, and 2010. (4) Emission growth across the 36 cities is fastest in Heilongjiang, followed by Liaoning and Jilin. (5) Tapio decoupling analysis shows overall positive decoupling in Heilongjiang, a decline in decoupling quality in Jilin, and no significant change in Liaoning. Finally, we recommend strategies to reduce emissions from the perspectives of government, industry, and residents. This research offers a quantitative foundation for achieving dual carbon goals and supports the implementation of policies focused on energy transition and sustainable urban planning.

Preprint ARTICLE | doi:10.20944/preprints202312.1397.v1

Quantitative Planning Credit Policy for Business Management

Todor Atanasov STOILOV, Krasimira Petrova Stoilova

Subject: Business, Economics And Management, Business And Management Keywords: algorithm for active credit management; stochastic number and values of payments, conditional formalization; resource allocation

Online: 19 December 2023 (05:56:17 CET)

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Preprint DATA DESCRIPTOR | doi:10.20944/preprints202304.1125.v1

Quantitative Metabolomic Dataset of Avian Eye Lenses

Ekaterina A. Zelentsova, Sofia S. Mariasina, Vadim V. Yanshole, Lyudmila V. Yanshole, Nataliya A. Osik, Kirill A. Sharshov, Yuri P. Tsentalovich

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: quantitative metabolomics; NMR spectroscopy; eye lens; birds

Online: 28 April 2023 (06:06:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0277.v2

A Quantitative Poisitive Energy District Definition with Contextual Targets

Simon Schneider, Thomas Zelger, David Sengl, José Baptista

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: Positive energy districts; PED definition; Context Factors; energy balance assessment

Online: 27 April 2023 (02:54:32 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202211.0096.v1

Quantitative Proteomics Approach to Characterize Cellular Reprogramming

Owen Hovey, Shanshan Zhong, Tomonori Kaneko, Shawn Li, Sally Ezra

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Proteomics; Phosphoproteomics; TMT; Phosphotyrosine enrichment; SP3

Online: 7 November 2022 (01:14:16 CET)

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Working Paper ARTICLE

Quantitative Evaluation of PACS Query/Retrieve Capabilities

Abderrazek Boufahja, Steven Nichols, Vincent Pangon

Subject: Engineering, Control And Systems Engineering Keywords: PACS; DICOM; Query; Retrieve; Quality

Online: 12 July 2020 (15:14:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0381.v1

Building-Level Wastewater Monitoring for COVID-19 Using Tampon Swabs and RT-LAMP for Rapid SARS-Cov-2 RNA Detection

Aaron Bivins, Megan Lott, Marlee Shaffer, Zhenyu Wu, Devin North, Erin Lipp, Kyle Bibby

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: SARS-CoV-2; wastewater monitoring; environmental surveillance; RT-LAMP; building-level; near-source; passive sampling

Online: 17 May 2021 (10:07:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202101.0264.v1

Measuring Time-Varying Effective Reproduction (Rt) Numbers for COVID-19 and Their Relationship with Movement Control Order (MCO) in Malaysia

Musa Kamarul Imran, Wan Nor Ariffin, Mohd Mohd Hafiz, Subhi Jamiluddin, Noor Atinah Ahmad, Chen Xin Wee, Tengku Muhammad Hanis, Awang Bulgiba

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Reproduction number; COVID-19; movement control order; epidemic curve

Online: 14 January 2021 (09:41:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1144.v1

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Gabriel S.M. Sousa, Rodrigo S. De Oliveira, Alex B. De Souza, Ruan C. Monteiro, Elaine P. T. E. Santo, Luciano C. Franco Filho, Denison L.O. Moraes, Sarah R. De Sá, Silvia H. M. Da Silva

Subject: Biology And Life Sciences, Other Keywords: chromoblastomycosis; phaeohyphomycosis; PCR-Multiplex; Herpotrichiellaceae; sequencing; phenotypic; molecular biology.

Online: 19 March 2024 (12:35:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1348.v1

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Pallavi Upadhyay, Jairus Reddy, Teddie Proctor, Oceane Sorel, Harita Veereshlingam, Manoj Gandhi, Vijay Singh

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Respiratory tract infections; COVID-19; influenza-like illness; multiplex PCR; syndromic panels

Online: 18 May 2023 (14:10:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0740.v1

Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow

Zachary P Morehouse, Caleb M Proctor, Gabriella L Ryan, Rodney J Nash

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Virus Detection; Viral Diagnostics; Diagnostics; SARS-CoV-2; Coronavirus; PCR

Online: 31 July 2020 (09:31:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0500.v1

Quantitative Visualization of the Interaction between Complement Component C1 and Immunoglobulin G: The Effect of CH1 Domain Deletion

Saeko Yanaka, Shigetaka Nishiguchi, Rina Yogo, Hiroki Watanabe, Jiana Shen, Hirokazu Yagi, Takayuki Uchihashi, and Koichi Kato

Subject: Biology And Life Sciences, Biophysics Keywords: immunoglobulin G; complement component C1; high-speed atomic force microscopy; CH1; CL

Online: 31 December 2021 (10:36:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0043.v1

Archaeological Evidence for Community Resilience and Sustainability: A Bibliometric and Quantitative Review

Matthew J. Jacobson

Subject: Arts And Humanities, Archaeology Keywords: archaeology; resilience; sustainability; citation analysis; collapse; climate change; agriculture; natural resources

Online: 2 November 2022 (04:19:44 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.0562.v1

Modulatory Impact of Oxidative Stress on Membrane Action Potentials in Pathophysiological States: A Comprehensive Review

CHITARANJAN MAHAPATRA, Ravindra Thakkar, Ravinder Kumar

Subject: Biology And Life Sciences, Biophysics Keywords: Oxidative stress; action potential; biophysics; ion channel; pathophysiology

Online: 8 August 2024 (12:14:52 CEST)

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