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Preprint ARTICLE | doi:10.20944/preprints201608.0038.v1

Reconstructing Historical VOC Concentrations in Drinking Water for Epidemiological Studies at a U.S. Military Base: Summary of Results

Morris L. Maslia, Mustafa M. Aral, Perri Z. Ruckart, Frank J. Bove

Subject: Environmental And Earth Sciences, Environmental Science Keywords: historical reconstruction; modeling; drinking water; water quality; VOC; epidemiological study; health study; Camp Lejeune

Online: 4 August 2016 (10:09:23 CEST)

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A U.S. government health agency conducted epidemiological studies to evaluate whether exposures to drinking water contaminated with volatile organic compounds at U.S. Marine Corps Base Camp Lejeune, North Carolina, were associated with increased health risks to children and adults. These health studies required knowledge of contaminant concentrations in drinking water—at monthly intervals—delivered to family housing, barracks, and other facilities within the study area. Because concentration data were limited or unavailable during much of the period of contamination (1950s–1985), the historical reconstruction process was used to quantify estimates of monthly mean contaminant-specific concentrations. This paper integrates many efforts, reports, and papers into a synthesis of the overall approach to, and results from, a drinking-water historical reconstruction study. Results show that at the Tarawa Terrace water treatment plant (WTP) reconstructed (simulated) tetrachloroethylene (PCE) concentrations reached a maximum monthly average value of 183 micrograms per liter (ug/L) compared to a one-time maximum measured value of 215 ug/L and exceeded the U.S. Environmental Protection Agency’s current maximum contaminant level (MCL) of 5 ug/L during the period November 1957–February 1987. At the Hadnot Point WTP, reconstructed trichloroethylene (TCE) concentrations reached a maximum monthly average value of 783 ug/L compared to a one-time maximum measured value of 1,400 ug/L during the period August 1953–December 1984. The Hadnot Point WTP also provided contaminated drinking water to the Holcomb Boulevard housing area continuously prior to June 1972, when the Holcomb Boulevard WTP came on line (maximum reconstructed TCE concentration of 32 ug/L) and intermittently during the period June 1972–February 1985 (maximum reconstructed TCE concentration of 66 ug/L). Applying the historical reconstruction process to quantify contaminant-specific monthly drinking-water concentrations is advantageous for epidemiological studies when compared to using the classical exposed versus unexposed approach.

Preprint COMMUNICATION | doi:10.20944/preprints202407.1576.v1

Development of Specific Anti-mouse Atypical Chemokine Receptor 4 Monoclonal Antibodies

Miu Hirose, Hiroyuki Suzuki, Rena Ubukata, Tomohiro Tanaka, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: mouse ACKR4; monoclonal antibody; peptide immunization; flow cytometry; western blotting

Online: 19 July 2024 (06:39:45 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202404.1066.v1

Development of Sensitive Anti-mouse CCR5 Monoclonal Antibodies Using the N-terminal Peptide Immunization

Rena Ubukata, Hiroyuki Suzuki, Tomohiro Tanaka, Tsunenori Ouchida, Guanjie Li, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: mouse CCR5; monoclonal antibody; peptide immunization; flow cytometry; western blotting

Online: 18 April 2024 (09:29:28 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202403.1166.v1

C₈Mab-21: A Novel Anti-human CCR8 Monoclonal Antibody for Flow Cytometry

Tomohiro Tanaka, Hiroyuki Suzuki, Guanjie Li, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CCR8, CBIS method, monoclonal antibody, flow cytometry

Online: 19 March 2024 (14:41:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1500.v1

Defucosylated Anti-CD44 Monoclonal Antibodies Exert Antitumor Activities against Esophageal Tumor Xenograft

Kenichiro Ishikawa, Hiroyuki Suzuki, Tomokazu Ohishi, Akira Ohkoshi, Mika K Kaneko, Yukio Katori, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CD44; monoclonal antibody; ADCC; CDC; esophageal tumor; antitumor activity

Online: 26 March 2024 (08:14:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0864.v1

Anti-HER2 cancer-specific mAb, H2Mab-250-hG₁ possesses higher complement-dependent cytotoxicity than trastuzumab

Hiroyuki Suzuki, Tomokazu Ohishi, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; cancer-specific monoclonal antibody; antitumor effect; complement-dependent cellular cytotoxicity

Online: 11 July 2024 (05:20:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201702.0054.v1

Identification of the Sixth Ig-like Domain of VCAM-1 as a Novel Therapeutic Target in Lung Cancer Cell Invasion

Mi Ra Kim, Ji Hye Jang, Chang Sik Park, Taek-Keun Kim, Youn-Jae Kim, Junho Chung, Hyunbo Shim, In Hyun Nam, Jung Min Han, Sukmook Lee

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: human antibody; invasion; lung cancer; therapeutic target; VCAM-1; VCAM-1-D6

Online: 15 February 2017 (10:45:16 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202406.0704.v1

Establishment of a Highly-sensitive Anti-EphB2 Monoclonal Antibody Eb2Mab-3 for Flow Cytometry

Rena Ubukata, Hiroyuki Suzuki, Miu Hirose, Hiroyuki Satofuka, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: EphB2; monoclonal antibody; Cell-Based Immunization and Screening; flow cytometry

Online: 11 June 2024 (11:40:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0238.v1

Anti-CD44 Variant 10 Monoclonal Antibody Exerts Antitumor Activity in Mouse Xenograft Models of Oral Squamous Cell Carcinomas

Kenichiro Ishikawa, Hiroyuki Suzuki, Tomokazu Ohishi, Guanjie Li, Tomohiro Tanaka, Manabu Kawada, Akira Ohkoshi, Mika K. Kaneko, Yukio Katori, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CD44 variant 10; monoclonal antibody; ADCC; CDC; oral cancer; antitumor activity

Online: 6 August 2024 (05:04:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1835.v1

Population-Based Incidence and Mortality Rates of Hematologic Malignancies regarding the Adult Population of Crete, Greece, and Analyses of Their Time Trends and Their Geographical Dis-Tribution: A First Approach from the Cancer Registry of Crete (CRC) for a 22-Year Time Period (1992–2013)

Stelios Lionakis, Dimitra Sifaki-Pistolla, Helen A. Papadaki, Dimitrios Mavroudis, Christos Lionis

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: hematologic neoplasms; incidence; mortality; cancer epidemiology; kaplan meier estimate; registries; spatio-temporal analysis

Online: 24 July 2024 (07:10:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0676.v1

The First Two Years of COVID-19 Hospitalization Characteristics and Costs: Results from the National Discharge Registry

Pierpaolo Ferrante

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: COVID-19; incidence; lethality; negative binomial; moving averages

Online: 12 March 2024 (08:20:48 CET)

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ABSTRACT Background: To date, the COVID-19 pandemic has been the foremost health concern for humankind in the new millennia. This paper aims to describe the related hospitalizations in Italy during the first two years of the health emergency. Design and methods: This is a retrospective, population-based study of Italian hospitalizations for patients diagnosed with COVID-19 during the 2020-2021 period, extracted from the National Hospital Discharge Registry. The outcome variables considered include hospital admissions, costs, and length of stay, among other hospitalization-level variables. Costs were estimated through the charges associated with the Diagnosis-Related Group and Major Diagnostic Categories coding system, plus an additional cost attributed to COVID-19 admissions. To these charges was added an extra cost defined by the ministry of health. Results: In Italy, there were 357,354 hospitalizations for COVID-19 attributed to 298,856 patients in 2020 and 399,043 hospitalizations for COVID-19 attributed to 333,447 patients in 2021. COVID-19 patients faced a transfer rate thrice that of other patients. Hospitalizations were concentrated predominantly in the northern regions, particularly in the first year. Hospitalization rates varied by age in a sine wave pattern, peaking in the youngest and oldest age groups, with mortality rates escalating with age— tending to remain below 3% for those under 44 but surging to 40% in individuals over 75. The financial impact of COVID-19 hospitalizations was substantial, with total costs reaching €3.97 billion in 2020 and €4.99 billion in 2021. Costs per admission and per day also rose, from €11,112 and €807 in 2020 to €12,503 and €844 in 2021, respectively. Excluding the added financial burden of COVID-19, costs would have been notably lower. Hospitalizations involving continuous invasive mechanical ventilation were particularly costly (about 24.000 euros x admission), reflecting the significant resources required for these treatments. Conclusion: Implementing a protective pad around the entire health system that leverages networks of family doctors and nurses, connected in real time to the entire health system, can be crucial. Such a network, tasked with monitoring the local epidemiological situation, protecting vulnerable individuals also through telehealth visits, and establishing a triage system for infections in the initial phase, may effectively help in managing virus spread and protecting hospitals from unpredictable waves of admission demand. Strengthening primary health care could be a decisive factor for the future and enhance the system's resilience in facing new challenges.

Preprint COMMUNICATION | doi:10.20944/preprints202404.0031.v1

Rationale and Design of the KOSovan Acute Coronary Syndrome (KOS-ACS) Registry

Gani Bajraktari, Shpend Elezi, Ibadete Bytyci, Pranvera Ibrahimi, Genc Abdyli, Edita Pllana-Pruthi, Rona Karahoda, Arlind Batalli, Afrim Poniku, Mentor Shatri, Drilon Gashi, Artan Bajraktari, Faik Shatri, Michael Y. Henein

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Acute myocardial infarction; mortalit; ; primary PCI

Online: 1 April 2024 (10:59:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0869.v1

Nutritional Trends in Cystic Fibrosis: Insights from the Italian Cystic Fibrosis Patient Registry

Donatello Salvatore, Rita Padoan, Annalisa Amato, Marco Salvatore, Giuseppe Campagna

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: cystic fibrosis, nutrition, obesity

Online: 15 April 2024 (08:07:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202405.1111.v1

Lung Cancer and Interstitial Lung Diseases

Fotios Drakopanagiotakis, Ekaterina Krauss, Ira Michailidou, Vasileios Drosos, Stavros Anevlavis, Andreas Günther, Paschalis Steiropoulos

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: interstitial lung disease; lung cancer; IPF

Online: 16 May 2024 (13:17:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.1711.v1

Dermatological Neoplastic Diseases Complicating Treatment with Monoclonal Antibody for Multiple Sclerosis

Floriana Bile, Maddalena Sparaco, Eleonora Ruocco, Giuseppina Miele, Elisabetta Maida, Renato Vele, Davide Mele, Simona Bonavita, Luigi Lavorgna

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Multiple Sclerosis; Skin cancer; Monoclonal antibodies.

Online: 22 July 2024 (11:47:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201609.0066.v1

Evaluation of Antibody Titre of Dogs Vaccinated against Canine Distemper in Jos, Plateau State

Kenneth Ikejiofor Ogbu

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: antibody titre; vaccination; dog; canine distemper virus; Jos

Online: 20 September 2016 (10:14:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1291.v1

Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea

Hwachul Shin, SangEun Lee, Myung-Min Choi, Hwajung Yi, Yoon-Seok Chung

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: smallpox; Vaccinia virus; smallpox vaccine; antibody titer; ELISA; bioterrorism

Online: 20 August 2024 (04:55:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0766.v1

Antibody Recognition to the Hyperglucosylated Adhesin Protein HMW1ct of Non Typeable Haemophilus Influenzae in Rett Syndrome

Feliciana Real Fernandez, Francesca Nuti, Lorenzo Altamore, Mattan Hurevich, Nir Qvit, Eduardo Ponticiello, Paolo Rovero, Joussef Hayek, Anna Maria Papini

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Adhesin protein; N-glucosylation; Antibodies; Immunoenzymatic assay; Rett syndrome ;

Online: 10 July 2024 (04:37:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1611.v1

Hematopoietic Cell Transplantation Trends and Outcomes in Canada: A Registry-based Cohort Study

Matthew D. Seftel, Ivan Pasic, Gaganvir Parmar, Oliver Bucher, David S. Allan, Sita Bhella, Kevin Anthony Hay, Olaweseun Ikuomola, Grace Musto, Anca Prica, Erin Richardson, Tony H Truong, Kristjan Paulson

Subject: Medicine And Pharmacology, Hematology Keywords: hematopoietic cell transplant; cellular therapy; health outcomes

Online: 25 October 2023 (11:50:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0166.v1

Infectious Diseases and Secondary Antibody Deficiency in Patients from a Mesoregion of São Paulo State

Luiz Euribel Prestes-Carneiro, Paula Andreia Martins Carrilho, Danimelle Francisco Honorato de Barros Torelli, Jose Antonio Nascimento Bressa, Ana Carolina Gomes Parizi, Pedro Henrique Meirelles Vieira, Fernanda Miranda Caliani Sa, Mauricio Domingues Ferreira

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Secondary Antibody Deficiency; hypo-γ -globulinemia; infectious diseases ;

Online: 4 March 2024 (15:50:34 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202404.1985.v1

Fatal Diffuse Alveolar Hemorrhage (DAH) Secondary to Monoclonal Antibody Therapy in a Young Patient with Ulcerative Colitis (UC): An Autopsy Case

Antonina Argo, Emanuele Lo Rito, Daniela Guadagnino, Giuseppe Davide Albano, Stefania Zerbo, Ginevra Malta

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: forensic pathology; inflammatory bowel diseases; immunosuppressant; Diffuse Alveolar Hemorrhage; monoclonal antibody, adverse reaction.

Online: 30 April 2024 (14:26:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.2079.v1

Barriers and Recommendations for Establishing a COVID-19 Patient Registry in a Regional Setting in the Philippines

Gerard Dumancas, Oliver Generalao, Hans Carlo Eugenio Rivera, Pia Regina Fatima Zamora, Jonathan Adam Rico, Fresthel Monica M. Climacosa, Salvador Eugenio Caoili, Romulo de Castro

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: COVID-19; patient registry; Philippines; Western Visayas; developing country

Online: 28 August 2024 (20:15:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201610.0012.v1

Bio-Resource Exchange: Study of Prevalence of Antibody Donation and Development of a Web Portal to Facilitate it

Sandeep Subramanian, Madhavi Ganapathiraju

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: data exchange; resource donations; text mining

Online: 5 October 2016 (15:08:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1481.v1

Flour Treatments Affect Gluten Protein Extractability, Secondary Structure, and Antibody Reactivity

Bruna Mattioni, Michael Tilley, Patricia Matos Scheuer, Niraldo Paulino, Umut Yucel, Donghai Wang, Alicia de Francisco

Subject: Chemistry And Materials Science, Food Chemistry Keywords: wheat; glutenin; gliadin; celiac disease ;

Online: 19 September 2024 (11:59:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201803.0232.v1

Cadastre-Land Registry Coordination in Spain: Application of Law 13/2015 and Its Effects

Carmen Femenia-Ribera, Gaspar Mora-Navarro

Subject: Social Sciences, Geography, Planning And Development Keywords: cadastre; land registry; notary; cartography; geomatic; coordination; GML; land surveyors

Online: 28 March 2018 (04:45:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1753.v1

Heterozygous SERPINA1 Defects and Their Impact on Clinical Manifestations of Patients with Predominantly Antibody Deficiencies

Styliani Sarrou, Ioanna Voulgaridi, Athanasia Fousika, Katerina Dadouli, Olympia Margaritopoulou, Ioannis Kakkas, Christos Hadjichristodoulou, Fani Kalala, Matthaios Speletas

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Predominantly antibody deficiencies; common variable immunodeficiency; alpha-1-antitrypsin; SERPINA1; liver disease.

Online: 28 March 2024 (09:58:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0235.v1

Public Land Action and Development of Land Registry System in Benin: Towards Effective Land Management?

Mawussi Kossi Nyalewo, Tchakouni Sondou, Karl Martial Nassi, Gbati Napo, Coffi C. Aholou

Subject: Social Sciences, Urban Studies And Planning Keywords: land actions; political will; cadastral project; land management; Benin

Online: 4 May 2023 (08:29:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0064.v1

MkRP: Multiple k Registry Placement for Fast Container Deployment on Edge Computing

CHUNGGEON SONG, HEONCHANG YU, Joon-Min Gil

Subject: Computer Science And Mathematics, Information Systems Keywords: container deployment; registry placement; edge computing

Online: 1 February 2024 (12:55:20 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.0099.v2

Future AI Will Be Able to Predict Antibody-Drug Conjugate Response in Oncology

Navid Sobhani, Alberto D'Angelo, Matteo Pittacolo, Giuseppina Mondani, Daniele Generali

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: artificial intelligence; antibody drug conjugates; prognostic; clinical trials

Online: 5 August 2024 (14:23:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1550.v1

Non-syndromic and Syndromic Defects in Children with Extracranial Germ Cell Tumors (eGCT): Data of 2,610 Children Registered with the German MAKEI 96/MAHO 98 Registry Compared to the General Population

Judit Schultewolter, Anke K. Rißmann, Dietrich Von Schweinitz, Michael Frühwald, Claudia Blattmann, Lars Fischer, Bjoern Soenke Lange, Rüdiger Wessalowski, Birgit Fröhlich, Wolfgang Behnisch, Irene Schmid, Harald Reinhard, Matthias Duerken, Patrick Hundsdoerfer, Martin Heimbrodt, Christian Vokuhl, Stefan Schönberger, Dominik T. Schneider, Guido Seitz, Ulrich Göbel, Rüdiger Von Kries, Heiko M. Reutter, Gabriele Calaminus

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: germ cell tumor; syndrome; non-syndromic defect

Online: 23 April 2024 (16:59:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0144.v1

IgG Antibody Titers against Ascaris lumbricoides, Strongyloides Stercolaris, and Toxocara canis in Venezuelan Patients with Asthma or COPD

Juan Bautista De Sanctis, Dolores Moreno, Nancy Larocca, Jenny Valetina Garmendia

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: COPD; asthma; atopy; eosinophils; IgE; Ascaris lumbricoides; Giardia lamblia; Strongyloides stercolaris; Toxocara canis

Online: 3 September 2024 (08:09:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0506.v1

Uses and Challenges of Antiviral Polyclonal and Monoclonal Antibody Therapies

Evi B. Struble, Jonathan M.O. Rawson, Tzanko Stantchev, Dorothy Scott, Marjorie Shapiro

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: antiviral therapy; antiviral antibodies; antibody combination therapy; antibody potency; potency assays

Online: 18 April 2023 (08:30:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0478.v1

You + ME Registry: A Research Platform to Facilitate Clinical and Therapeutic Discoveries in ME/CFS and Related Diseases

Allison Ramiller, Kathleen Mudie, Elle Seibert, Sadie Whittaker

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome; Long COVID; data acquisition source; post-infectious; longitudinal cohort study; patient-powered

Online: 25 November 2021 (13:51:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0015.v1

Immunization of Nile tilapia (Oreochromis niloticus) Broodstock With Tilapia Lake Virus (TiLV) Inactivated Vaccines Elicits Protective Antibody and Passive Maternal Antibody Transfer

Thao Thu Mai, Pattanapon Kayansamruaj, Chayanit Soontara, Pattarawit Kerddee, Dinh-Hung Nguyen, Saengchan Senapin, Janina Z. Costa, Jorge del- Pozo, Kim D. Thompson, Channarong Rodkhum, Ha Thanh Dong

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: tilapia broodstock; inactivated vaccines; maternal passive immunity; antibody

Online: 4 January 2022 (15:41:19 CET)

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Tilapia lake virus (TiLV), a major pathogen of farmed tilapia, is known to be vertically transmitted. Here, we hypothesize that Nile tilapia (Oreochromis niloticus) broodstock immunized with a TiLV inactivated vaccine can mount a protective antibody response and passively transfer maternal antibodies to their fertilized eggs and larvae. To test this hypothesis, three groups of tilapia broodstock, each containing 4 males and 8 females, were immunized with either a heat-killed TiLV vaccine (HKV), a formalin-killed TiLV vaccine (FKV) (both administered at 3.6 ×106 TCID50 per fish), or with L15 medium. Booster vaccination with the same vaccines was given 3-weeks later, and mating took place 1 week thereafter. Broodstock blood sera, fertilized eggs and larvae were collected from 6-14 weeks post-primary vaccination for measurement of TiLV-specific antibody (anti-TiLV IgM) levels. In parallel, passive immunization using sera from the immunized female broodstock was administered to naïve tilapia juveniles to assess if antibodies induced in immunized broodstock were protective. The results showed that anti-TiLV IgM was produced in the majority of both male and female broodstock vaccinated with either the HKV or FKV and that and that these antibodies could be detected in the fertilized eggs and larvae from vaccinated broodstock. Higher levels of maternal antibody were observed in fertilized eggs from broodstock vaccinated with HKV than those vaccinated with FKV. Low levels of TiLV-IgM were detected in some of the 1-3-day old larvae but were undetectable in 7-14-day old larvae from the vaccinated broodstock, indicating a short persistence of TiLV-IgM in larvae. Moreover, passive immunization proved that antibodies elicited by TiLV vaccination were able to confer 85% to 90% protection against TiLV challenge in naïve juvenile tilapia. In conclusion, immunization of tilapia broodstock with TiLV vaccines could be a potential strategy for the prevention of TiLV in tilapia fertilized eggs and larvae, with HKV appearing to be more promising than FKV for maternal vaccination.

Preprint ARTICLE | doi:10.20944/preprints202405.1072.v1

Serum Antibody Titers against Distemper, Parvovirus and In-fectious Hepatitis in Dogs from Central Spain

JOSE L. BLANCO, ELENA PARRA, SILVIA RUBIES, GUSTAVO ORTIZ-DIEZ, MARTA E GARCIA

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Canine Distemper Virus; Canine Parvovirus; Infectious Hepatitis Virus; IgG level; Vaccicheck

Online: 16 May 2024 (11:55:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0348.v1

Accuracy of Anti-SARS-CoV2 Antibody in Comparison with Surrogate Viral Neutralization Test in Person Living with HIV, Systemic Lupus Erythematosus and Chronic Kidney Disease

Marita Restie Tiara, Chrisan Bimo Prayuda, Tara Titian Maulidya, Hofiya Djauhari, Dadang Suhendar, Rudi Wisaksana, Laniyati Hamijoyo, Rudi Supriyadi, Agnes Rengga Indrati, Bachti Alisjahbana

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: COVID-19; vaccination; antibody; immunoassay; neutralization antibody

Online: 4 April 2024 (05:53:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1949.v1

Elderly Patients with Idiopathic Pulmonary Hypertension: Clinical Characteristics, Survival and Risk-Stratification in a Single-Center Prospective Registry

Natalia S. Goncharova, Kirill B. Lapshin, Aelita V. Berezina, Maria A. Simakova, Alexander O. Marichev, Irina S. Zlobina, Narek V. Marukyan, Kirill N. Malikov, Alexandra S. Aseeva, Vadim V. Zaitsev, Olga M. Moiseeva

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: idiopathic pulmonary arterial hypertension; comorbidities; survival; risk stratification

Online: 26 December 2023 (06:57:04 CET)

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Introduction: The predictive value of the risk stratification scales in elderly patients with IPAH might differ from that in younger. It is unknown whether young and older IPAH patients have the same survival dependence on PAH-specific therapy numbers. The aim of the study was to evaluate the prognostic relevance of risk stratification scales and PAH medication numbers in elderly in comparison with young IPAH patients. Material and methods: 119 patients from a prospective single-center PAH registry were divided on to group I <60 years old (n=89) and group II ≥60 years old (n=30). ESC/ERS, REVEAL, and REVEAL 2.0 risk stratification scores were assessed at baseline, as well as H2FpEF- score and survival at follow-up. Results: During a mean follow-up period of 2.9 years (1.63; 6.0) 42 (35.3%) patients died; at 1, 2, 3, 5, 7, and 10 years survival was 95%, 88.6%, 78.5%, 61.7%, 48.5% and 33.7%, respectively. No survival difference was observed between the two groups despite the use of monotherapy in elderly patients. The best predictive value exhibited the REVEAL in group II (IPAH patients ≥60 years) - AUC 0.73 (0.56-0.91), p = 0.03; and in patients with LHD comorbidities in the entire cohort - AUC 0.73 (0.59-0.87), p<0.009. Factors independently associated with death in the entire cohort were CKD (p=0.01, HR 0.2), the right-to-left ventricle dimension ratio (p=0.0047, HR 5.97), and NT-proBNP >1400 pg/ml (p=0.008, HR 3.18). Conclusion: Risk stratification in elderly IPAH patients requires a fundamentally different approach than that of younger patients, taking into account the initial limitations in physical performance and comorbidities that interfere with current assessment scores. The REVEAL score reliably stratifies patients at any age and LHD comorbidities. The initial monotherapy seems to be reasonable in patients over 60 years. Selection tools for initial combination PAH therapy in older IPAH patients with comorbidities need to be validated in prospective observational studies.

Preprint ARTICLE | doi:10.20944/preprints202010.0624.v1

Real Life Clinical Management and Survival in Cutaneous Malignant Melanoma: the Italian Clinical National Melanoma Registry (CNMR) Experience

Anna Crispo, Maria Teresa Corradin, Erika Giulioni, Antonella Vecchiato, Paolo Del Fiore, Paola Queirolo, Francesco Spagnolo, Vito Vanella, Corrado Caracò, Giulio Tosti, Elisabetta Pennacchioli, Giuseppe Giudice, Eleonora Nacchiero, Pietro Quaglino, Simone Ribero, Monica Giordano, Desire Marussi, Stefania Barruscotti, Michele Guida, Vincenzo De Giorgi, Marcella Occelli, Federica Grosso, Giuseppe Cairo, Alessandro Gatti, Daniela Massa, Laura Atzori, Nicola Calvani, Tommaso Fabrizio, Giuseppe Mastrangelo, Federica Toffolutti, Egidio Celentano, Mario Budroni, Sara Gandini, Carlo Riccardo Rossi, Alessandro A. E. Testori, Giuseppe Palmieri, Paolo A. Ascierto

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: medical record systems; cutaneous malignant melanoma; survival analysis; immunotherapy

Online: 29 October 2020 (16:02:44 CET)

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Background: Cutaneous malignant melanoma (CMM) is one of the most aggressive types of skin cancer. Currently, innovative approaches such as target therapies and immunotherapies have been introduced in clinical practice for the treatment of metastatic CMM. Data of clinical trials and real life studies that evaluate the outcomes of these therapeutic associations are necessary to establish their clinical utility. The aim of this study is to investigate the types of oncological treatments employed in the real-life clinical management of patients with advanced CMM in several Italian centers which are part of the Clinical National Melanoma Registry (CNMR), and the oncological outcomes obtained. Methods: CNMR collects data of patients with a histologically confirmed diagnosis of primary CMM treated in one of the 38 Italian institutions (hospitals, research institutes, etc.) participating in the network. Melanoma-specific survival and Overall survival were calculated. Kaplan-Meier curves and medians of OS and 95% CI are presented overall and by immunotherapy and target treatments. The Log-rank test compared curves by treatments. Multivariate Cox regression models were used to estimate the hazard ratios adjusting for confounders and other prognostic factors. Results: The median follow-up time was 36 months (range 1.2-185.1). 787 CMM were included in the analysis with completed information about therapies.Global immunotherapy showed a significant improved survival compared with all other therapies (p=0.001). 75% was the highest reduction of death reached by nivolumab/pembrolizumab immunotherapy (anti-PD1 HR=0.25 95% CI 0.14-0.42), globally immunotherapy was significantly associated with improved survival, either for anti-CTL A4 monotherapy or combined with anti-PD1 (HR=0.47;95% CI 0.33-0.66 and HR=0.26; 95% CI 0.15-0.46, respectively). Conclusions: The nivolumab/pembrolizumab and the combination of ipilimumab can be considered the best therapy to improve survival in a real-world-population. The CNMR can complement clinical registries with the intent of improving cancer management and standardizing cancer treatment.

Preprint ARTICLE | doi:10.20944/preprints202305.1285.v1

Characteristics of Phase IV Clinical Trials in Oncology: An Analysis Using the ClinicalTrials.gov Registry Data

Brandon Michael Henry, Giuseppe Lippi, Ameen Nasser, Patryk Ostrowski

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: postmarketing surveillance; pharmacovigilance; registry data

Online: 18 May 2023 (07:18:52 CEST)

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Preprint DATASET | doi:10.20944/preprints202003.0011.v1

Extracting the Interfacial Electrostatic Features from Experimentally Determined Antigen and/or Antibody-Related Structures inside Protein Data Bank for Machine Learning-Based Antibody Design

Wei Li

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: antigen-antibody complex structure; interfacial electrostatic feature; Machine Learning-Based Antibody Design; Protein Data Bank

Online: 1 March 2020 (12:39:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1425.v1

A Nationwide Study of the Delayed Diagnosis and the Clinical Manifestations of Predominantly Antibody Deficiencies and CTLA4-Mediated Immune Dysregulation Syndrome in Greece

Androniki Kapousouzi, Fani Kalala, Styliani Sarrou, Evangelia Farmaki, Nikolaos Antonakos, Ioannis Kakkas, Alexandra Kourakli, Vasiliki Labropoulou, Charikleia Kelaidi, Georgia Tsiouma, Maria Dimou, Theodoros Vassilakopoulos, MICHAEL VOULGARELIS, Ilias Onoufriadis, Eleni Papadimitriou, Sophia Polychronopoulou, Evangelos J Giamarellos-Bourboulis, Argiris Symeonidis, Christos Hadjichristodoulou, Anastasios E. Germenis, Matthaios Speletas

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: predominantly antibody deficiencies, common variable immunodeficiency, IgA deficiency, IgG subclass deficiency, CTLA4, diagnosis

Online: 26 February 2024 (12:46:08 CET)

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Predominantly antibody deficiencies (PAD) represent the most common type of primary immu-nodeficiencies in humans, characterized by a wide variation in disease onset, clinical manifesta-tions, complications and outcome. Considering that the prevalence of PAD in Greece is unknown and there is limited knowledge on clinical and laboratory characteristics of affected patients, we conducted a nationwide study, including 153 patients (male/female: 66/87; median age: 43.0 years; range: 7.0-77.0) diagnosed and/or followed-up between April 2007 to September 2023. Furthermore, we classified our cohort into five Groups according to their medical history, im-munoglobulin levels, and CTLA4-mutational status: 123 had common variable immunodeficien-cy (CVID), 12 with CVID-like disease (with a history of a previous B-cell depletion immunother-apy for autoimmune or malignant disease), seven with combined IgA and IgG subclass deficien-cies, five with immune dysregulation syndrome and CTLA4 defects, and six with unclassified hypogammaglobulinemia. We demonstrated a remarkable delay of PAD diagnosis, several years after the onset of related symptoms (median: 9.0 years, range: 0-43.0). Family history of PAD was only 11.8%, with the majority of patients considered sporadic cases. Most patients were diag-nosed in the context of a diagnostic work-up for recurrent infections, or recurrent/resistant auto-immune cytopenias. Interestingly, ten patients (5.6%) had no history of infection, diagnosed due to either recurrent/resistant autoimmunity, or during a work-up of their medical/family history. Remarkable findings included an increased prevalence of lymphoproliferation (60.1%) and atopy (24.2%), while 39 patients (25.5%) developed bronchiectasis and 16 (10.5%) granulomatous dis-ease. Patients with CTLA4 defects displayed a variable clinical phenotype at diagnosis, with four patients diagnosed during a work-up of re-current infections, while one also displayed a massive splenomegaly. Cancer was a common complication in our cohort (25 patients, 16.3%), with B-cell malignancies representing the most common neoplasms (56.7%). Our findings indicate the necessity of awareness about PAD and their complications, aiming for early diagnosis and appropriate management of affected patients

Preprint BRIEF REPORT | doi:10.20944/preprints202305.0563.v2

A Descriptive Analysis of Sleeve Gastrectomy in Japan Based on National Registry by Japanese Society for Treatment of Obesity

Shinichi Okazumi, Takashi Oshiro, Akira Sasaki, Hisahiro Matsubara, Ichiro Tatsuno

Subject: Arts And Humanities, Humanities Keywords: national registry; bariatric surgery; sleeve gastrectomy; safety; efficacy

Online: 19 June 2023 (07:36:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1176.v1

A Biomarker-Based Characterisation of EMT and Neuroendocrine Differentiation States in Pancreatic and Small Cell Hypercalcemic Ovarian Tumor Cells and Their Modulation by Transforming Growth Factor-β and Bone Morphogenetic Protein-7

Hendrik Ungefroren, Juliane von der Ohe, Rüdiger Braun, Jörg Schrader, Hendrik Lehnert, Jens-Uwe Marquardt, Björn Konukiewitz, Ralf Hass

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pancreatic ductal adenocarcinoma; epithelial-mesenchymal transition; mesenchymal-epithelial transition; neuroendocrine differentiation; TGF-β

Online: 16 July 2024 (00:23:09 CEST)

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Pancreatic ductal adenocarcinoma (PDAC) represents an extremely aggressive type of cancer due in part to early invasion and metastasis, which in turn involves epithelial-mesenchymal transition (EMT) of the tumor cells. Prompted by the discovery that two PDAC-derived tumor cell lines of the quasi-mesenchymal subtype (PANC-1, MIA PaCa-2) exhibit neuroendocrine differentiation (NED), we asked whether NED is associated with EMT. Using quantitative real-time PCR and immunoblot analysis, we initially verified endogenous expression of various NED markers, i.e., chromogranin A (CHGA), synaptophysin (SYP), somatostatin receptor 2 (SSTR2) and SSTR5 in PANC-1 and MIA PaCa-2 cells. In HPDE6c7 normal pancreatic duct epithelial cells and in BxPC-3, another PDAC cell line with an epithelial phenotype, the expression of CHGA, SYP and neuron-specific enolase 2 (NSE) was either undetectable or much lower than in PANC-1 and MIA PaCa-2 cells. Parental cultures of PANC-1 cells exhibit EM plasticity (EMP) and harbor clonal subpopulations with different EMT phenotypes [Ungefroren et al 2022]. Monitoring the NED state of mesenchymal and epithelial PANC-1 subclones showed that the NE phenotype is more pronounced in M-type clones when compared to E-type ones. Inducing EMT in parental cultures of PANC-1 cells by treatment with transforming growth factor (TGF)-β1 repressed epithelial genes and co-induced mesenchymal and NED genes, except for SSTR5. Surprisingly, treatment with bone morphogenetic protein (BMP)-7 differentially affected gene expression in PANC-1 and MIA PaCa-2 cells. It synergised with TGF-β1 in the induction of vimentin, SNAIL, SSTR2 and NSE but antagonised it in the regulation of CHGA and SSTR5. From these data, we conclude that in EMT of PDAC cells mesenchymal and NED markers are co-regulated, and that mesenchymal-epithelial transition (MET) is associated with a loss of both the mesenchymal and NED phenotypes. Analysing NED in another tumor type, small cell carcinoma of the ovary hypercalcemic type (SCCOHT), revealed that two model cell lines of this disease (SCCOHT-1, BIN-67) do express CDH1, SNAI1, VIM, CHGA, SYP, ENO2 and SSTR2, but that in contrast to BMP-7 none of these genes was transcriptionally regulated by TGF-β1. We conclude that in PDAC-derived tumor cells, NED is closely linked to EMT and TGF-β signaling, which may have implications for the therapeutic use of TGF-β inhibitors in PDAC management.

Preprint REVIEW | doi:10.20944/preprints202305.1084.v1

Lysosomal Cleavable Peptide Linkers in Antibody-Drug Conjugates

Seetharamsing Balamkundu, Chuan Fa Liu

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: antibody-drug conjugate; lysosome; cathepsin; legumain; self-immolation; payload release; peptidomimetic; linker

Online: 16 May 2023 (04:51:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0343.v1

The Spatial-Temporal Distribution of Chronic Lymphatic Filariasis in Indonesia: A 18-Year Registry-Based Analysis

Dewi Nur Aisyah, Zisis Kozlakidis, Haniena Diva, Siti Tarmizi, Lita Sianipar, Eksi Wijayanti, Ajie Avicena, Wiku Bakti Bawono Adisasmito

Subject: Medicine And Pharmacology, Tropical Medicine Keywords: Lymphatic Filariasis; Indonesia; National Surveillance; Registry; BELKAGA

Online: 22 September 2022 (10:41:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0073.v1

Risk Prediction of Second Primary Endometrial Cancer in Obese Women: A Hospital-based Cancer Registry Study

Chi-Chang Chang, Chun-Chia Chen, Chalong Cheewakriangkrai, Ying Chen Chen, Shun-Fa Yang

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: second primary cancers (SPCs); endometrial cancer (EC); risk prediction.

Online: 2 July 2021 (15:54:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0052.v1

Efficacy, Pharmacokinetic and Toxicity Profiles of a Broadly SARS-CoV2 Neutralizing Antibody

Silvia Godínez-Palma, Edith González-González, Frida Ramírez-Villedas, Circe Garzón-Guzmán, Luis Vallejo-Castillo, Gregorio Carballo-Uicab, Gabriel Marcelín-Jiménez, Dany Batista, Sonia M. Pérez-Tapia, Juan C. Almagro

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: COVID-19; therapeutic antibody; SARS-CoV-2 Delta; SARS-CoV-2 Omicron; toxicology

Online: 1 August 2023 (11:27:40 CEST)

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Preprint REVIEW | doi:10.20944/preprints202008.0166.v1

Antibody Response and Therapy in COVID-19 Patients: Significance in Vaccine Development

Ligong Lu, Hui Zhang, Meixiao Zhan, Jun Jiang, Hua Yin, Danielle J. Dauphars, Shi-You Li, Yong Li, You-Wen He

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: COVID-19, seroconversion, neutralizing antibody, spike protein, SARS-CoV-2, convalescent plasma, vaccine

Online: 6 August 2020 (12:16:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1603.v1

Perioperative Analgesia in Crisis Situation SARS-CoV-2. Patient Characteristics in COVID-19 in Pain Out Registry

María A. Pérez-Herrero, Manuel Carrasco, Berta Velasco, Sara Cocho, Carla del Rey, Hermann Ribera

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: analgesia; COVID-19; pain; postoperative pain; SARS-CoV2

Online: 22 July 2024 (03:10:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0788.v1

Concurrent Traumatic Brain Injury with Craniofacial Trauma: A 10-year Analysis of a Single Institution’s Trauma Registry

Yuchen You, Javier Romero, Graal Diaz, Robin Evans

Subject: Medicine And Pharmacology, Surgery Keywords: Craniofacial trauma; Traumatic Brain Injury; Concurrent diagnosis

Online: 12 June 2023 (07:31:13 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0738.v1

Current State Of Monoclonal Antibody Therapy for Transthyretin Amyloidosis

Avi Chakrabartty

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: transthyretin; amyloidosis; antibody therapy; protein structure; cryo-electron microscopy

Online: 12 August 2024 (02:57:10 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.0307.v1

Overcoming Variants Resistance in Monoclonal Antibody for Antiviral Therapy

Mujahed Mustafa, Awad Alzebair, Hanan Mohamednoor, Ahmed Mohammed

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: monoclonal antibodies; variants resistance; bispecific antibodies; engineered antibodies; antiviral therapy

Online: 6 November 2023 (10:38:14 CET)

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Preprint REVIEW | doi:10.20944/preprints201905.0326.v1

Monoclonal Antibody as an Emerging Therapies for Ischemic Stroke

Demi Woods, Qian Jiang, Xiang-Ping Chu

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: stroke; antibody therapy; monoclonal antibody; inflammation; acid-sensing ion channel; receptor; growth factors

Online: 28 May 2019 (10:05:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.0759.v1

Clinical Significance and Remaining Issues of Anti‐ HBc Antibody and HBV Core‐Related Antigen

Yoshihiko Yano, Itsuko Sato, Takamitsu Imanishi, Ryutaro Yoshida, Takanori Matsuura, Yoshihide Ueda, Yuzo Kodama

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: anti-HBc antibody; HBV core-related antigen; HBV

Online: 14 February 2024 (09:11:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0237.v1

Incidence and Risk Factors for Cerebrovascular-Specific Mortality in Patients with Colorectal Cancer: A Registry-Based Cohort Study Involving 563298 Patients

Wei Huang, Ming Tang, Yun-Liang Chen, Tao-Lan Zhang, Tao Hong, Jing Li, Guo-Hua Lv, Yiguo Yan, Zhi-Hua Ouyang, Mingxiang Zou

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Colorectal cancer; Cerebrovascular-specific mortality; Cerebrovascular-specific diseases; Incidence; Risk factors

Online: 12 November 2021 (16:47:45 CET)

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Preprint REVIEW | doi:10.20944/preprints202010.0607.v1

Selection Strategies for Tissue-Specific Antibody Identification Using Phage Display and Naïve Libraries

Célestine Mairaville, Pierre Martineau

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: immunohistochemistry; phage display; monoclonal antibody

Online: 29 October 2020 (10:25:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202010.0494.v1

Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates Against HIV Env-Expressing Cells

Mohammad Sadraeian, Calise Bahou, Edgar Ferreira da Cruz, Luíz Mário Ramos Janini, Ricardo Sobhie Diaz, Ross W. Boyle, Vijay Chudasama, Francisco Eduardo Gontijo Guimarães

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: HIV immunotherapy; photoimmunotherapy; photodynamic Therapy; porphyrin; phthalocyanine; HIV-infected cell; monoclonal antibody

Online: 23 October 2020 (14:55:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0241.v1

Downregulation of miRNA-26a by HIV-1 Enhances CD59 Expression and Packaging Impacting Virus Susceptibility to Antibody-Dependent Complement-Mediated Lysis

Nicolas Bellini, Chengyu Ye, Oluwaseun Ajibola, Thomas T Murooka, Robert Lodge, Eric A Cohen

Subject: Biology And Life Sciences, Virology Keywords: CD59; HIV-1; complement- mediated lysis; miRNA-26a; innate immunity; viral evasion

Online: 5 June 2024 (12:35:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0175.v1

Domains and Categories of Needs in Long-term Follow-up of Adult Cancer Survivors: A Scoping Review of Systematic Reviews

Nicolas Sperisen, Dimitri Kohler, Nicole Steck, Pierre-Yves Dietrich, Elisabetta Rapiti

Subject: Public Health And Healthcare, Other Keywords: cancer survivors; needs; follow-up; supportive care; information; health system; assessment

Online: 2 April 2024 (09:41:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0906.v5

Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H2Mab-250/H2CasMab-2 for Breast Cancers

Mika K Kaneko, Hiroyuki Suzuki, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; cancer-specific monoclonal antibody; screening; epitope; flow cytometry

Online: 4 January 2024 (11:30:55 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202310.0453.v1

PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry

Tsunenori Ouchida, Tomohiro Tanaka, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: podoplanin; PDPN; giraffe; Immunohistochemistry

Online: 9 October 2023 (04:24:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1236.v1

Investigation Detection of Antibody+Antigen Complexes by SOI-FET Biosensor

Vladimir Generalov, Anastasia Cheremiskina, Aleksandr Glukhov, Victoria Grabezhova, Margarita Kruchinina, Aleksandr Safatov

Subject: Biology And Life Sciences, Other Keywords: biosensor; SOI-FET; transistor; detection; virus; particles; probability; plausibility

Online: 16 June 2023 (11:49:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0207.v1

Fast Confirmation of Antibody Identity by MALDI-TOF-MS Fingerprints

Georg Tscheuschner, Timm Schwaar, Michael G. Weller

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Antibody ID; antibody registry; Research Resource Identifier; RRID; reproducibility; quality control; documentation; traceability; clones; biochemical reagents; diagnostics; immunoassays; ELISA; western blot; immunohistochemistry; microarray; biosensor

Online: 15 February 2020 (15:46:27 CET)

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Thousands of antibodies for diagnostic and other analytical purposes are on the market. However, it is often difficult to identify duplicates, reagent changes, and to assign the correct original publications to an antibody. This slows down scientific progress and might even be a cause of irreproducible research and a waste of resources. Recently, activities were started to suggest the sole use of recombinant antibodies in combination with the open communication of their sequence. In this case, such uncertainties should be eliminated. Unfortunately, this approach seems to be rather a long-term vision since the development and manufacturing of recombinant antibodies remain quite expensive in the foreseeable future. Also, nearly all commercial antibody suppliers may be reluctant to publish the sequence of their antibodies, since they fear counterfeiting. De-novo sequencing of antibodies is also not feasible today for a reagent user without access to the hybridoma clone. Nevertheless, it seems to be crucial for any scientist to have the opportunity to identify an antibody undoubtedly to guarantee the traceability of any research activity using antibodies from a third party as a tool. For this purpose, we developed a method for the identification of antibodies based on a MALDI-TOF-MS fingerprint. To circumvent lengthy denaturation, reduction, alkylation, and enzymatic digestion steps, the fragmentation was performed with a simple formic acid hydrolysis step. Eighty-nine unknown monoclonal antibodies were used for this study to examine the feasibility of this approach. Although the molecular assignment of peaks was rarely possible, antibodies could be easily recognized in a blinded test, simply from their mass-spectral fingerprint. A general protocol is given, which could be used without any optimization to generate fingerprints for a database. We want to propose that in most scientific projects relying critically on antibody reagents, such a fingerprint should be established to prove and document the identity of the used antibodies and to assign a specific reagent to a datasheet of a commercial supplier, a public database record or an antibody ID.

Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0288.v1

SARS-CoV-2 Seroepidemiology and Antibody Levels in Children during BA.5 Predominance Period

Filippos Filippatos, Elizabeth-Barbara Tatsi, Maria-Myrto Dourdouna, Alexandra Margeli, Vasiliki Syriopoulou, Athanasios Michos

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: SARS-CoV-2; COVID-19; Children; BA.5 Omicron variant; seroepidemiology

Online: 5 February 2024 (15:09:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0748.v1

Preclinical Pharmacokinetics and Biodistribution of LR004, a Novel Antiepidermal Growth Factor Receptor Monoclonal Antibody

Ying Zheng, Guifang Dou, Shuchen Liu, Zhiyun Meng, Eric I. Tsao, Gang Yu, Xiaoxia Zhu, Ruolan Gu, Zhuona Wu, Yunbo Sun, Peng Han, Hui Gan

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: monoclonal antibody; EGFR; tumor-targeting; pharmacokinetics; radionuclide-based biodistribution; nanoSPECT/CT

Online: 12 December 2023 (05:11:26 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202004.0024.v1

Emergence of Drift Variants That May Affect COVID-19 Vaccine Development and Antibody Treatment

Takahiko Koyama, Dilhan Weeraratne, Jane L. Snowdon, Laxmi Parida

Subject: Biology And Life Sciences, Virology Keywords: COVID-19; SARS-nCoV-2; vaccine; antibody; immune escape; variant; spike protein; genomic drift; convalescent plasma

Online: 3 April 2020 (04:24:52 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202003.0138.v1

Medical Countermeasures Analysis of 2019-nCoV and Vaccine Risks for Antibody-Dependent Enhancement (ADE)

Darrell O. Ricke, Robert W. Malone

Subject: Biology And Life Sciences, Virology Keywords: 2019-nCoV; SARS-CoV-2; COVID-19; ADE; antibody depedendent enhancement

Online: 8 March 2020 (15:35:27 CET)

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Background: In 80% of patients, COVID-19 presents as mild disease^1,2. 20% of cases develop severe (13%) or critical (6%) illness. More severe forms of COVID-19 present as clinical severe acute respiratory syndrome, but include a T-predominant lymphopenia³, high circulating levels of proinflammatory cytokines and chemokines, accumulation of neutrophils and macrophages in lungs, and immune dysregulation including immunosuppression⁴. Methods: All major SARS-CoV-2 proteins were characterized using an amino acid residue variation analysis method. Results predict that most SARS-CoV-2 proteins are evolutionary constrained, with the exception of the spike (S) protein extended outer surface. Results were interpreted based on known SARS-like coronavirus virology and pathophysiology, with a focus on medical countermeasure development implications. Findings: Non-neutralizing antibodies to variable S domains may enable an alternative infection pathway via Fc receptor-mediated uptake. This may be a gating event for the immune response dysregulation observed in more severe COVID-19 disease. Prior studies involving vaccine candidates for FCoV^5,6 SARS-CoV-1^7-10 and Middle East Respiratory Syndrome coronavirus (MERS-CoV) ¹¹ demonstrate vaccination-induced antibody-dependent enhancement of disease (ADE), including infection of phagocytic antigen presenting cells (APC). T effector cells are believed to play an important role in controlling coronavirus infection; pan-T depletion is present in severe COVID-19 disease³ and may be accelerated by APC infection. Sequence and structural conservation of S motifs suggests that SARS and MERS vaccine ADE risks may foreshadow SARS-CoV-2 S-based vaccine risks. Autophagy inhibitors may reduce APC infection and T-cell depletion¹² ¹³. Amino acid residue variation analysis identifies multiple constrained domains suitable as T cell vaccine targets. Evolutionary constraints on proven antiviral drug targets present in SARS-CoV-1 and SARS-CoV-2 may reduce risk of developing antiviral drug escape mutants. Interpretation: Safety testing of COVID-19 S protein-based B cell vaccines in animal models is strongly encouraged prior to clinical trials to reduce risk of ADE upon virus exposure.

Preprint ARTICLE | doi:10.20944/preprints202310.0080.v1

Prescription Practices of Cannabinoids in Children with Cere-bral Palsy Worldwide - A Survey of the Swiss Cerebral Palsy Registry

Sandra Hunziker, Federico Morosoli, Kathrin Zuercher, Anne Tscherter, Sebastian Grunt

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cannabis; cannabinoids; tetrahydrocannabinol; cannabidiol; cerebral palsy; pediatrics

Online: 3 October 2023 (02:58:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0693.v1

Contemporary Status of Acute Myocardial Infarction in Korean Patients: Korean Registry of Acute Myocardial Infarction for Regional Cardiocerebrovascular Centers

Rock Bum Kim, Jin Yong Hwang, Hyun-Woong Park, Ae-Young Her, Jang-Hoon Lee, Moo Hyun Kim, Chang Hwan Yoon, Jae Young Cho, Sung-Il Woo, Yongcheol Kim, Jae-Young Han, Joon Hyouk Choi, Song Yi Kim, Si-Wan Choi, Sung Ju Jee, Sang Yeub Lee, Ki-Bum Won, Kyeong-Soo Park, Dae Woo Hyun

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: acute myocardial infarction; case fatality; registry

Online: 27 November 2020 (14:12:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1615.v1

Ultrasound-Assisted, Catheter-Directed Thrombolysis for Acute Intermediate/High Risk Pulmonary Embolism: Design of the Multicenter USAT IH-PE Registry and Preliminary Results

Claudia Colombo, Nicolò Capsoni, Filippo Russo, Mario Iannaccone, Marianna Adamo, Giovanna Viola, Ilaria Emanuela Bossi, Luca Villanova, Chiara Tognola, Camilla Curci, Francesco Morelli, Rossella Guerrieri, Lucia Occhi, Giuliano Chizzola, Antonio Rampoldi, Francesco Musca, Giuseppe De Nittis, Mario Galli, Giacomo Boccuzzi, Daniele Savio, Davide Bernasconi, Luciana D’Angelo, Andrea Garascia, Alaide Chieffo, Matteo Montorfano, Fabrizio Oliva, Alice Sacco

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: pulmonary embolism; ultrasound assisted; catheter-directed thrombolysis; pulmonary hypertension

Online: 21 December 2023 (06:19:38 CET)

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Preprint REVIEW | doi:10.20944/preprints202109.0501.v2

Sagacious Epitope Selection for Vaccines, and Both Antibody-Based Therapeutics and Diagnostics: Tips From Virology and Oncology

Samuel Ken-En Gan, Ser-Xian Phua, Joshua Yi Yeo

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Antigen Selection; Epitope Selection; Antibody Targeting; Epitope Accessibility; Antibody Engineering; Protein Engineering; Drug Targeting; Model-Informed Drug Discovery and Development

Online: 3 November 2021 (08:26:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1444.v1

Impaired Development of Collagen Antibody-Induced Arthritis in Rab44-Deficient Mice

Yu Yamaguchi, Tomoko Kadowaki, Eiko Sakai, Mayuko Noguromi, Shun Oyakawa, Takayuki Tsukuba

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Rab44; collagen antibody-induced arthritis; immune cells; osteoclasts

Online: 18 September 2024 (15:11:03 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202312.2059.v1

Establishment of a Novel Anti-mouse CCR1 Monoclonal Antibody C₁Mab-6

Tsunenori Ouchida, Yu Isoda, Tomohiro Tanaka, Mika K. Kaneko, Hiroyuki Suzuki, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: mouse CCR1, CBIS method, monoclonal antibody, flow cytometry

Online: 27 December 2023 (07:06:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.0232.v1

Antibody Drug Conjugates: Ushering in a New Era of Cancer Therapy

Joshua Hurwitz, Lucy Roxana Haggstrom, Elgene Lim

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Antibody-drug conjugate; cancer; clinical trials

Online: 5 June 2023 (04:13:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0048.v1

Regulatory T Cell Depletion Using A CRISPR Fc-Optimized CD25 Antibody

Marit J. Van Elsas, Johan M.S. Van der Schoot, Alexander Bartels, Kas Steuten, Duco Van Dalen, Zacharias Wijfjes, Carl G. Figdor, Thorbald Van Hall, Sjoerd H. Van der Burg, Martijn Verdoes, Ferenc A. Scheeren

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: antibody; immunotherapy; CRISPR/HDR; FC optimization; hybridoma

Online: 2 August 2022 (08:10:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0360.v1

Development of A Novel Anti-Epcam Monoclonal Antibody for Various Applications

Guanjie Li, Hiroyuki Suzuki, Teizo Asano, Tomohiro Tanaka, Hiroyoshi Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: EpCAM; monoclonal antibody; recombinant antibody; colorectal carcinoma

Online: 28 March 2022 (10:11:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints201902.0043.v1

HER2 Directed Antibody-Drug-Conjugates beyond T-DM1 in Breast Cancer

Gabriel Rinnerthaler, Simon Peter Gampenrieder, Richard Greil

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: ADC; HM2-MMAE; (vic-)trastuzumab duocarmazine; Trastuzumab deruxtecan; TAK-522; Trastuzumab emtansine; anti-HER2/PBD-MA; HER2 low; HER2-low; mode of action

Online: 4 February 2019 (17:01:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints201808.0431.v1

Importance of HIV cell-to-cell transmission - implications for neutralizing antibody

Tuckweng Kok, Peng Li

Subject: Biology And Life Sciences, Virology Keywords: HIV, cell-to-cell transmission, designer antigens, neutralizing antibody, cell-free, high multiplicity of infection

Online: 24 August 2018 (10:25:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0658.v1

Revolutionizing Cancer Treatment - The Emergence and Impact of Antibody-Drug Conjugates (ADCs): A Systematic Review

Christopher Korban, Omar Fayaz, Aryan Kohli, Christian Chung

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: antibody-drug conjugates; cancer; monoclonal antibodies

Online: 10 April 2024 (03:04:44 CEST)

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Antibody-drug conjugates (ADCs), a groundbreaking advancement in cancer therapy, synergistically combine the precision of monoclonal antibodies with the potent destruction capabilities of cytotoxic drugs, signifying a new era in the targeted oncological treatments. This comprehensive paper delves into the evolving field of antibody-drug conjugates (ADCs) and their pivotal role in cancer therapy. We have conducted an in-depth exploration of the structural components of ADCs, including target antigens, monoclonal antibodies, chemical linkers, and cytotoxic payloads, each playing a crucial role in determining the efficacy and safety profile of these novel therapies. A detailed market analysis reveals the leading pharmaceutical companies shaping the ADC landscape, reflecting the growing interest and potential in this therapeutic approach. We further dissect the engineering intricacies of ADCs, focusing on their ability to target specific cancer cell antigens while minimizing off-target effects, thus offering a more focused treatment with reduced systemic side effects compared to traditional chemotherapy. This approach holds promise in a heightened therapeutic index and decreased toxicity. The mechanisms and effector functions of ADCs are explored, highlight- ing their multifaceted strategy in combating cancer. We include a comprehensive look at how ADCs operate from initial blood circulation to the induction of apoptosis in cancer cells. We also review several key ADCs currently in use or under investigation, offering insights into their unique mechanisms of action, pharmacokinetics, and clinical implications. The discussion extends to drug creation modalities, focusing on innovative conjugation techniques that enhance ADC homogeneity and therapeutic efficacy. In conclusion, the paper underscores the transformative potential of ADCs in the realm of cancer treatment, marking a shift towards more precise and targeted therapeutic approaches. By offering a thorough analysis of ADC technology, this paper sheds light on the prospects of these groundbreaking therapies in the ongoing fight against cancer.

Preprint COMMUNICATION | doi:10.20944/preprints202312.0369.v1

Epitope Mapping of an Anti-Mouse CD39 Monoclonal Antibody using PA Scanning and RIEDL Scanning

Hiroyuki Suzuki, Yuki Okada, Tomohiro Tanaka, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: mouse CD39; monoclonal antibody; epitope; PA scanning; RIEDL scanning

Online: 6 December 2023 (10:38:01 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202401.0889.v1

Impact of Mechanical Circulatory Support Timing on Outcomes in Myocardial Infarction-Related Cardiogenic Shock: Subanalysis of the PREPARE CS Registry

Dan Mihai Prunea, Eva Bachl, Lukas Herold, Sadeek Sidney Kanoun Schnur, Sascha Pätzold, Siegfried Altmanninger-Sock, Gudrun Angelika Sommer, Theresa Glantschnig, Ewald Kolesnik, Markus Wallner, Klemens Ablasser, Heiko Bugger, Eva Buschmann, Andreas Praschk, Friedrich M. Fruhwald, Albrecht Schmidt, Dirk Von Lewinski, Gabor Toth-Gayor

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: cardiogenic shock; mechanical circulatory support; myocardial infarction; mechanical circulatory support timing; in-hospital mortality

Online: 11 January 2024 (08:10:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.2148.v1

Real-World Clinical Characterization of Polycythemia Vera Patients From a Prospective Registry in Portugal: Is Resistance to Hydroxyurea a Reality?

Maria Sarmento, Marta Duarte, Sandra Ponte, Juan Sanchez, Diana Roriz, Laura Fernandes, Maria José Monteiro Silva, Judite Pacheco, Gisela Ferreira, Jorge Freitas, Inês Costa, Daniel Brás

Subject: Medicine And Pharmacology, Hematology Keywords: disease management; hemorragic events; hydroxyurea; resistance; symptomatic evaluation; patient-reported outcomes; thrombotic events; polycythemia vera

Online: 30 May 2023 (13:11:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1894.v1

Improving Pepper Inbreds for Resistance to Pepper Yellow Leaf Curl Thailand Virus (PepYLCTHV) through Challenged Inoculations

Nattika Kumsee, Patcharaporn Suwor, Montinee Teerarak, Wen-Shi Tsai, Suchila Techawongstien, Tanyarat Tainta, Sanjeet Kumar, Nakarin Jeeatid, Orawan Chatchawankanphanich, Somsak Kramchote

Subject: Biology And Life Sciences, Horticulture Keywords: Artificial screening; Begomovirus; Breeding for resistance; Germplasm resistance to PepYLCTHV

Online: 29 May 2024 (09:03:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0661.v1

Survival and Recurrence of Endocarditis Following Mechanical vs. Biological Aortic Valve Replacement in Patients Aged 40 to 65 Years: Data from the INFECT-REGISTRY

Antonio Salsano, Michele Di Mauro, Laura Labate, Alessandro Della Corte, Federica Lo Presti, Michele De Bonis, Cinzia Trumello, Mauro Rinaldi, Erik Cura Stura, Guglielmo Actis Dato, Giuseppe Punta, Francesco Nicolini, Davide Carino, Carlo De Vincentiis, Andrea Garatti, Giangiuseppe Cappabianca, Andrea M Musazzi, Diego Cugola, Maurizio Merlo, Davide Pacini, Gianluca Folesani, Sandro Sponga, Igor Vendramin, Alberto Pilozzi Casado, Francesco Rosato, Elisa Mikus, Carlo Savini, Francesco Onorati, Giovanni Battista Luciani, Roberto Scrofani, Francesco Epifani, Francesco Musumeci, Antonio Lio, Andrea Colli, Giosuè Falcetta, Salvatore Nicolardi, Salvatore Zaccaria, Enrico Vizzardi, Antonio Pantaleo, Giuseppe Minniti, Emmanuel Villa, Margherita Dalla Tomba, Francesco Pollari, Fabio Barili, Alessandro Parolari, Roberto Lorusso, Francesco Santini

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: endocarditis; aortic valve replacement; prosthetic heart valve.

Online: 9 November 2023 (15:01:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1836.v3

The Combination of Binding-Avidity of Ovomucoid-Specific IgE Antibody and Specific IgG4 Antibody Can Predict Positive Outcome of Oral Food Challenge during Stepwise Slow Oral Immunotherapy in Children with Hen’s Egg Allergy

Shoichiro Taniuchi, Rika Sakai, Takashi Nishida, Meguru Goma, Masatoshi Mitomori, Aya Imaide, Masahiro Enomoto, Masamitsu Nishino, Yo Okizuka, Hiroshi Kido

Subject: Biology And Life Sciences, Food Science And Technology Keywords: oral immunotherapy; hen’s egg allergy; IgE binding-avidity; ovomucoid specific IgE; ovomucoid specific IgG4; oral food challenge

Online: 31 May 2023 (02:28:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0714.v3

Analysis of Beyfortus® (Nirsevimab) Immunization Campaign: Effectiveness, Biases, and ADE Risks in RSV Prevention

Helene Banoun

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: nirsevimab; beyfortus; ADE; RSV; bronchiolitis; mAbs; therapeutic monoclonal antibody

Online: 13 September 2024 (08:24:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1864.v1

The Enigma of the ʹSmokerʹs Paradoxʹ: Results from a Single‐Center Registry of Patients with STEMI Undergoing Primary Percutaneous Coronary Intervention

Umberto Paradossi, Alberto Ranieri De Caterina, Giancarlo Trimarchi, Filippo Dossi, Mario Raccis, Giacomo Bianchi, Cataldo Palmieri, Cesare De Gregorio, Giuseppe Andò, Sergio Berti

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: smoker’ s paradox; lifestyle risk factors; STEMI; ischemic time; primary PCI

Online: 26 January 2024 (07:21:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1354.v1

Encapsulation of Human IgG Antibody in PLGA Microparticles Fabricated by Coaxial Electrospray

Karla Nataly Robles, John Paul Libanati, Richard Mu, Todd Giorgio

Subject: Chemistry And Materials Science, Biomaterials Keywords: antibody encapsulation; sustained-release; PLGA; immunotherapy; local administration; microparticles; coaxial electrospray

Online: 21 May 2024 (09:12:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1063.v1

Chimeric Viruses Enable Study of Antibody Responses to Human Rotaviruses in Mice

Sarah Woodyear, Tawny L Chandler, Takahiro Kawagishi, Tom M Lonergan, Vanshika A Patel, Caitlin A Williams, Sallie R Permar, Siyuan Ding, Sarah L Caddy

Subject: Biology And Life Sciences, Virology Keywords: Rotavirus; antibody; reverse genetics; vaccine

Online: 16 May 2024 (08:09:28 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202311.0501.v2

Cx₁Mab-1: A Novel Anti-mouse CXCR1 Monoclonal Antibody for Flow Cytometry

Guanjie Li, Tomohiro Tanaka, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: mouse CXCR1, CBIS method, monoclonal antibody, flow cytometry

Online: 22 December 2023 (07:53:29 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202311.0805.v1

Cx₃Mab-4: a Novel Anti-mouse CXCR3 Monoclonal Antibody for Flow Cytometry

Tsunenori Ouchida, Yu Isoda, Tomohiro Tanaka, Mika K Kaneko, Hiroyuki Suzuki, Yukinari Kato

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: mouse CXCR3; monoclonal antibody; CBIS method

Online: 14 November 2023 (05:37:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0392.v1

Development of a Novel Anti-CD44 Variant 4 Monoclonal Antibody, C₄₄Mab-108

Hiroyuki Suzuki, Tomohiro Tanaka, Teizo Asano, Nohara Goto, Mika K. Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: CD44; CD44 variant 4; monoclonal antibody; flow cytometry; immunohistochemistry

Online: 21 December 2022 (07:40:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints201911.0023.v1

Antibody Screening by Microarray Technology – Direct Identification of Selective High-Affinity Clones

Martin Paul, Michael G. Weller

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: monoclonal antibodies; Mabs; fusion; false positives; hapten immunoassays; competitive immunoassays; ELISA; antibody validation; antibody quality; microarray; hybridoma technology; linker recognition; high-throughput screening; HTS; heterology concept

Online: 3 November 2019 (17:00:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints201808.0370.v1

Successive Optimization of a Homogeneous Immunoassay for Antibody Detection in Viral Infections

Lina S. Franco, Vladimiro Mujica, Joseph N. Blattman, Antonio A. García

Subject: Biology And Life Sciences, Virology Keywords: gold nanoparticles; homogeneous immunoassay; antibody detection; virus infection; Dengue Protein E

Online: 21 August 2018 (05:57:40 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1578.v1

Vaccine-Induced Immune Thrombotic Thrombocytopenia—Clinicopathologic Features and New Perspectives on Anti-PF4 Antibody-Mediated Disorders

Yi Zhang, Anna-Lise Bissola, Jared Treverton, Michael Hack, Mark Lychacz, Sarah Kwok, Addi Arnold, Ishac Nazy

Subject: Medicine And Pharmacology, Hematology Keywords: vaccine-induced immune thrombotic thrombocytopenia; adenoviral-vector based COVID-19 vaccines; platelet-factor 4; anti-PF4 antibody-mediated disorders; platelet; SARS-CoV-2

Online: 20 December 2023 (16:45:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0538.v1

Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System

Tim Wendlandt, Claudia Koch, Beate Britz, Anke Liedek, Nora Schmidt, Stefan Werner, Yuri Gleba, Farnoosh Vahidpour, Melanie Welden, Arshak Poghossian, Michael J. Schöning, Fabian J. Eber, Holger Jeske, Christina Wege

Subject: Biology And Life Sciences, Virology Keywords: turnip vein clearing virus (TVCV); tobacco mosaic virus (TMV); tobamovirus; enzyme nanocarrier; protein A; antibody; enzyme cascade; glucose oxidase (GOx); horseradish peroxidase (HRP); biosensor

Online: 7 August 2023 (12:23:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2090.v1

Safety, Immunogenicity, and Antibody Persistence of COVID-19 Boosters in BBIP-CorV Immunized Individuals with Comorbidities

Fatemeh Ashrafian, Fahimeh Bagheri Amiri, Anahita Bavand, Mahsan Zali, Mona Sadat Larijani, Amitis Ramezani

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: COVID-19 vaccine; booster; underlying diseases; antibody

Online: 29 June 2023 (10:10:56 CEST)

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Data on immunogenicity, immune response persistency, and safety of COVID-19 boosters in patients with comorbidities are limited. Therefore, we aimed to evaluate and compare three different boosters in individuals who received two doses of the BBIBP-CorV vaccine including underlying diseases and healthy cases as control. One hundred forty subjects including 63 ones with at least one of three underlying diseases (UD) (including obesity, hypertension, and diabetes mellitus) and 77 healthy ones (HC) who had received a booster dose (either PastoCovac Plus or PastoCovac or BBIBP-CorV) were enrolled. The presence of SARS-CoV-2 antibodies was assessed before the booster injection and 28, 60, 90, and 180 days after it. Moreover, the adverse events (AEs) were recorded on days 7 and 21 post-booster shot for evaluating safety outcomes. Significantly increased titers of anti-spike, anti-RBD, and neutralizing antibodies were observed in both UD and HC groups 28 days after the booster dose, although the titer rise of anti-spike IgG and anti-RBD IgG was insignificantly inferior in the UD group compared to the HC group. All antibodies’ titers declined, with no significant differences between the two groups over time. Notably, all specific antibodies persisted up to 180 days; particularly the neutralizing antibody in both groups. Furthermore, no significant difference in antibody levels was observed between each UD subgroup and the HC group, except for neutralizing antibodies in the hypertension sub-group. PastoCovac Plus and PastoCovac boosters induced higher antibodies’ fold rise in UD individuals than BBIBP-CorV booster recipients. Safety outcomes did not show any serious AEs after the booster injection. The overall incidence of AEs post the booster injection was higher in the UD group than the HC group. Furthermore, the highest systemic AEs rate was reported in the UD group receiving the BBIBP-CorV booster. In conclusion, administration of COVID-19 boosters can equally induce robust and persistent humoral immune responses in individuals with or without UD primarily vaccinated with 2-doses of the BBIBP-CorV. Protein-based boosters with higher antibodies' fold rise and lower AEs in individuals with comorbidities might be considered a better choice for these individuals.

Preprint ARTICLE | doi:10.20944/preprints202104.0101.v1

Generation and Activity Evaluation of a Mouse-Human Immunoglobulin G1 Chimeric Antibody to Histoplasma capsulatum HSP60

Ágata Nogueira D'Áurea Moura, Scott J. Garforth, Leandro Buffoni Roque da Silva, Darien Woodley, Filipe Vieira Barbalho, Jose A. Quiroz, Steven C. Almo, Jonathan R. Lai, Joshua Daniel Nosanchuk, Carlos Pelleschi Taborda

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Mouse-Human Chimeric Antibody; Immunotherapy; Infectious diseases; Histoplasmosis; Paracoccidioidomycosis; Histoplasma capsulatum; Paracoccidioides lutzii

Online: 5 April 2021 (11:33:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0381.v2

Identification of 22 N-glycosites on Spike Glycoprotein of SARS-CoV-2 and Accessible Surface Glycopeptide Motifs: Implications on Vaccination and Antibody Therapeutics

Dapeng Zhou, Ruibing Qi, Wen Zhang, Xiaoxu Tian, Chao Peng

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV2; corona virus; glycopeptide; N-linked glycans; mass spectrometry; antibody; cryo-EM structure; crystal structures; epitope prediction

Online: 13 April 2020 (11:09:29 CEST)

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