In this article, the biocatalytic oxidation of ethanol into acetaldehyde was studied using a catalase entrapped within monolithic polyampholyte cryogel, p(APTAC-co-AMPS), derived from an anionic monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS), and a cationic monomer, (3-acrylamidopropyl) trimethylammonium chloride (APTAC) as catalyst. Macroporous polyampholyte cryogels containing various amounts of catalase were synthesized in situ under cryo-polymerization conditions at a molar ratio of monomers [APTAC]:[AMPS] = 75:25 mol.% in the presence of 10 mol.% cross-linking agent, N,N-methylenebisacrylamide (MBAA). The conversion of ethanol into acetaldehyde in good-to-high yields was observed in flow-through and batch type reactors under optimal conditions: at T = 10-20 C, pH = 7.1, [C2H5OH]:[H2O2] = 50:50 vol.%. According to SEM image the pore sizes of p(AMPS-co-APTAC) cryogel vary from 15 to 55 μm. The catalytic activity of catalase entrapped within monolithic polyampholyte cryogel in the conversion of ethanol into acetaldehyde was evaluated through the determination of such kinetic parameters as Michaelis constant (Km), the maximum enzymatic rate (Vmax), activation energy (Ea), turnover number (TON) and turnover frequency (TOF). The catalase encapsulated within monolithic polyampholyte cryogel exhibits a high conversion of ethanol into acetaldehyde. The advantages and disadvantages of flow-through and batch type reactors were highlighted.

This paper presents the results of investigations into the pyrolysis of waste polypropylene in a laboratory fixed-bed batch reactor. The experiments were designed and verified in such a way as to allow the application of the Response Surface Methodology (RSM) in the development of an empirical mathematical model that quantifies the impacts mentioned above. The influence of the mass of the raw material (50, 100 and 150 g), together with the reactor temperature (450, 475 and 500 °C) and the reaction time (45, 50 and 75 min) was examined. It has been shown that the mass of the raw material, i.e. the filling volume of the reactor, has a significant influence on the pyrolysis oil yield. This influence exceeds the influence of reactor temperature and reaction time. This was explained by observing the temperature change inside the reactor at three different spots at the bottom, middle and top of the reactor. Recorded temperature diagrams show that with greater masses of feedstock, local overheating occurs in the middle part of the reactor, which leads to overcracking of vapor products, and from there to an increased formation of non-condensable gases, i.e. reduced yield of pyrolytic oil.

The hydrogenation of CO2 is a reaction of key technological and environmental importance, as it contributes to the sustainable production of fuels while assisting in the reduction of a major greenhouse gas. The reaction has been studied extensively in the literature, with noble metal catalysts receiving significant attention within the catalysis and electrocatalysis communities. In this respect, the Electrochemical Promotion of Catalysis (EPOC) has been applied successfully to the CO2 hydrogenation reaction to improve the catalytic activity and selectivity of conductive films supported on solid electrolytes. However, designing an effective electrocatalytic reactor remains a challenge due to the connections required between the electrodes and the external potentiostat/galvanostat. This drawback could be alleviated if the catalytic reaction occurs in a reactor that simultaneously operates as a power generator. In this work, the Electrochemical Promotion of the CO2 hydrogenation reaction in a low temperature solid oxide fuel cell (SOFC) reactor is studied using yttria-stabilized zirconia (YSZ) and a platinum (Pt) catalyst. The system has been studied in two distinct operation modes: (i) when the necessary energy for the electrochemical promotion is produced through the parallel reaction of H2 oxidation (galvanic operation) and (ii) when a galvanostat/potentiostat is used to impose the necessary potential (electrolytic operation).

The fine chemical and pharmaceutical sectors are starting to advocate the use of flow chemistry due to reasons such as EHS, environmental, efficiency, cost saving and regulatory compliance. The use of trickle bed or fixed bed system could replace a batch autoclave typically used for hydrogenation reactions. However, there are few studies which detail the process from laboratory proof of concept through design to commercial realization. This study, using the production of 1,3-cyclohexanedione from catalytic hydrogenation of resorcinol as a case study demonstrates how the laboratory-scale recycle trickle bed can be used for catalyst screening and selection. Further, design data are generated by operation over a range of design superficial velocities and operating pressure that are used to derive a design correlation that is then used to specify a single stream plant at a level of definition consistent with a Preliminary Design for capital cost estimation. Finally, the further actions required in terms of data generation to increase the level of definition and confidence to a Sanction Grade or Final Design are discussed.

The per capita availability of freshwater resources on a global scale is gradually decreasing due to population growth, rapid urbanization, industrialization, and modern lifestyles. In this situation, wastewater management becomes a major concern. Conventional treatment processes used to treat wastewater discharges are not adequate for the advanced removal of pollutants, especially refractory organic pollutants, which are mainly organic compounds that are not easily biodegradable in the environment. The use of photocatalytic membrane reactors (PMRs) for tertiary wastewater treatment has attracted much interest in recent periods, and various configurations have been investigated. The performance of a PMR using an iron doped titanium photo catalyst and Polysulfone-based polymeric membrane for municipal wastewater treatment was evaluated under natural and simulated solar light. Obtained results proved that solar driven PMRs can remove organic loading (COD) with high efficiencies and may represent a suitable alternative for the tertiary treatment step of municipal wastewater.

The second article in the series presents the application of the Smoothed Particle Hydrodynamics (SPH) method to modelling of batch crystallisation in stirred tanks. A methodology to integrate the population balance equations (PBE) in parallel and independently from the Navier-Stokes equations is demonstrated. The benefits of the proposed methodology in terms of computational requirements, accuracy and availability of the crystal size distribution are discussed. The specific formulation of the SPH equations where the resulting system of ordinary differential equations is solved using the weighted contributions rather than numerically by solving a linear system of equations allows for massive parallelisation and a very loose coupling of the population balance and the fluid dynamics. It has been demonstrated, that the population balance equations can be solved on a Shared Memory Architecture (SMA) system using the OpenMP interface while the fluid dynamics equations being computed independently on a General Purpose Graphics Processing Unit (GPGPU) using the NVidia CUDA technology. This way, a significant portion of the computational overhead due to the large number of additional transport equations resulting from the discretisation of the population balance was removed: the SPH simulation coupled with 200 population balance equations was only 40% slower compared to SPH-only simulation. Two methods for the solution of population balance equations that preserve full crystal size distribution were implemented: discretised population balance (DPB) and method of characteristics (MOCH). The DPB equations are solved using the high-resolution finite-volume method with flux limiter and the effect of a large number of different flux limiters have been investigated. Both methods were validated using the case studies from the literature where an analytical solution can be derived. The developed models were applied to a numerical solution of coupled computational fluid dynamics and population balance equations to model a batch crystallization process. The effect of the hydrodynamics on the local temperature/supersaturation and the resulting crystal size distribution was captured and compared to the ideal mixing case. The simulation results from the DPB and MOCH methods were compared in terms of computational requirements and accuracy and MOCH selected as computationally more efficient and accurate.

This study evaluated batch fermentation modes, namely, separate hydrolysis and fermentation (SHF), Quasi-simultaneous saccharification and fermentation (Q-SSF), and simultaneous saccharification and fermentation (SSF), and fermentation conditions, i.e., enzyme and yeast loadings, nutrient supplementation and sterilization, on high titer bioethanol production from SPORL-pretreated Douglas-fir forest residue without detoxification. The result indicated Q-SSF and SSF were obviously superior to SHF operation in terms of ethanol yield. The enzyme loading showed a strong positive correlation between enzyme loading and the ethanol yield. The nutrient supplementation and sterility was not necessary for ethanol production from SPORL-pretreated Douglas-fir. The yeast loading showed no significant influence on the ethanol yield for typical SSF conditions. The terminal ethanol titer of 43.2 g/L, or 75.1% theoretical based on glucose, mannose, and xylose theoretical was achieved when SSF was conducted at the condition of following: whole slurry solids loading of 15%, enzyme loading of 20 FPU/g glucan, 1.8 g/kg (wet) yeast loading, without nutrition supplementation and sterilization, at 38°C, on shake flask at 150 rpm for 96h. It is believed that with mechanical mixing, enzyme loading can be substantially reduced with affect ethanol yield by using a long fermentation time.

To conduct high-precision and high-resolution numerical simulation of complex flow structures in turbomachinery, a high-order finite volume weighted essentially non-oscillatory (WENO) scheme for the large eddy simulation (LES) is improved and embedded into the three-dimensional viscous unsteady CFD solver NUAA-Turbo. Firstly, the spectral characteristics and unsteady convergence of the improved WENO scheme are studied. Compared with the classical WENO scheme, the improved WENO scheme has better dissipation and dispersion characteristics and faster convergence speed. Then, the coefficient CW of the Wall-Adapting Local Eddy-viscosity (WALE) model is calibrated by decaying homogeneous isotropic turbulence (DHIT) test case. Finally, the scheme is applied to conduct high-precision LES calculations of turbulent boundary layer flow, supersonic compression corner flow, and low-pressure turbine cascade separation flow. The numerical simulation results show that this WENO scheme has excellent shock wave capture ability and turbulence resolution and can capture more flow field details with less mesh size, greatly reducing the computational cost and laying a foundation for large-scale engineering application of LES.

The building envelope has the most significant contribution in the reduction of the building energy consumption. Application of new, alternative and improved materials and systems has an important impact on the buildings performances. This paper is focused on the thermal transmittance, as an indicator of the thermal conductance of the construction element. It includes comparisons of the U-values, calculated by software, with those measured in situ on three representative façade walls. The walls have been constructed with the new wall system Fragmat NZ-1, a new product in Macedonian buildings. This research provides basic information on the thermal transmittance of the system. The results of the analysis show that the in situ measuring is a useful tool in validation of the precision of analytically calculated values, since it was difficult to obtain precise results from the analytical calculations only, especially when the layers are with non-uniform thickness.

This paper concentrates on the general birth-death processes with two different types of catastrophes. The Laplace transform of transition probability function for birth-death processes with two-type catastrophes are is successfully expressed with the Laplace transform of transition probability function of the birth-death processes without catastrophe. The first effective catastrophe occurrence time is considered. The Laplace transform of its probability density function, expectation and variance are obtained.

In this paper, a new axial-type magnetic gear with auxiliary flux-enhancing structure (AFS-AMG) is proposed. Compared to conventional AFS-AMG, it has the higher torque density and higher permanent magnet (PM) utilization factor. Firstly, the design rules and operating principles of proposed AFS-AMG are elaborated. Then, the mapping relation between the radial-type magnetic gears (RMGs) and AMGs are elucidated. Compared to its counterpart in RMGs, AFS-AMGs achieve small size. Then, the geometrical parameters of AFS-AMGs are optimized to obtain the better electromagnetic performances, where the torque density per volume and per PM volume is adopted as the evaluation standard. Finally, three different AMG topologies are constructed in finite element analysis (FEA) software for comparison. It is proven that the AFS-AMG has the largest torque density per volume and per PM volume.

The volume of vehicular traffic in large cities has increased in recent years, the devices that collect vehicular GPS data such as cameras, GPS receivers and others generate millions of records at every instant of time generating problems in processing and storage of these data which becomes important for researchers. Intelligent Transportation Systems perform vehicle monitoring and control by collecting GPS trajectories, this large volume of information is necessary to have an optimal storage process. Its processing by means of compression techniques and simplification algorithms allow to reduce the necessary storage space. This paper presents a GPS trajectory simplification algorithm that considers noise reduction, point simplification and analysis of road network information. The results obtained on two data sets from the cities of California and Beijing are satisfactory, achieving a higher compression ratio without affecting data quality

In this paper, we study the well-posedness and asymptotics of a one-dimensional thermoelastic laminated beam system either with or without structural damping, where the heat conduction is given by Fourier's law effective in the rotation angle displacements. We show that the system is well-posed by using Lumer-Philips theorem, and prove that the system is exponentially stable if and only if the wave speeds are equal, by using the perturbed energy method and Gearhart-Herbst-Prüss-Huang theorem. Furthermore, we show that the system with structural damping is polynomially stable provided that the wave speeds are not equal, by using the second-order energy method.

The assortment of low-cost 3D printers for “in-practice” use, e.g., for clear aligner therapies, is ever increasing. To address concerns about the accuracy of orthodontic models produced on such printers when cost efficient modes of 3D printing are employed, this study examined the effect of print model body type and print angulation on accuracy. Six printing-configuration groups were included: 2 model types (solid or hollow shell) combined with 3 print angles (0°, 70°, or 90°) with 10 models/group; all models were printed with 100-µm layer thickness using a digital light processing-based 3-dimensional printer. Eleven selected structures and distances were measured on the printed models with a digital microscope and compared to the same measures on a digitized master model. The clinically-acceptability range was set at ±0.25 mm difference from the master model for single tooth measurements (intra-tooth) and ±0.5 mm for cross-arch measurements (inter-tooth). For individual measurements across all models, 98% fell within clinical acceptability. For mean measurements within each model group, only canine height for the shell-0° model had a mean difference (0.26 mm ± 0.03) outside the clinically acceptable range for intra-tooth measurements. Standard deviations for all intra-tooth measurements were within 0.07 mm. While none of the mean inter-tooth measurements exceeded the acceptability range, the standard deviations were larger (0.04 to 0.30 mm). Accuracy of orthodontic models for clear aligner therapies was not impacted beyond the clinically acceptable range when altering model body type and print angulation to improve efficiency of 3D printing. These findings suggest greater flexibility of the practitioner to alter print settings to address time and cost efficiency in various clinical scenarios and still maintain clinically acceptable model accuracy.

Training supervised machine learning models requires labeled examples. A judicious choice of examples is helpful when there is a significant cost associated with assigning labels. This article improves upon a promising extant method – Batch-mode Expected Model Change Maximization (B-EMCM) method – for selecting examples to be labeled for regression problems. Specifically, it develops and evaluates alternate strategies for adaptively selecting batch size in B-EMCM. By determining the cumulative error that occurs from the estimation of the stochastic gradient descent, a stop criteria for each iteration of the batch can be specified to ensure that selected candidates are the most beneficial to model learning. This new methodology is compared to B-EMCM via mean absolute error and root mean square error over ten iterations benchmarked against machine learning data sets. Using multiple data sets and metrics across all methods, one variation of AB-EMCM, the max bound of the accumulated error (AB-EMCM Max), showed the best results for an adaptive batch approach. It achieved better root mean squared error (RMSE) and mean absolute error (MAE) than the other adaptive and non-adaptive batch methods while reaching the result in nearly the same number of iterations as the non-adaptive batch methods.

Currently, a large part of the wells for agricultural use located in the state of Sonora are overexploited, which generates a high degree of saline intrusion and abandonment by nearby communities. In this paper the temperature effect on the final concentration of permeate water was evaluated through voltage and intel concentration variations in a batch electrodialysis reversal process (EDR), in order to identify optimal operating conditions with emphasis on the reduction of energy consumption and cost of desalinated water. Thirty-six samples were prepared: eighteen 2,000 mg/L total dissolved solids (TDS) samples and eighteen 5,000 mg/L TDS samples; brackish well water of 639 mg/L TDS and synthetic salt were mixed to obtain those concentrations. 3 different temperatures (25, 30, 35 °C) and 2 different voltages (10 and 20 V) were tested for each sample. The best salt removal occurred in the 20 V arrays, with 18.34% higher removal for 2,000 mg/L TDS experiments and 25.05% for 5,000 mg/L experiments (average between the 25 to 35 °C tests). Temperature positively affected EDR, especially in the experiments at 10 V voltage, where increasing 10 °C increased its efficiency by 10.83% and 24.69% for 2,000 and 5,000 mg/L TDS, respectively. Energy consumption was lower with increasing temperature (35 °C), as it decreased by 1.405% and 1.613% for 2,000 and 5,000 mg/L TDS concentrations, respectively (average between 10 and 20 V tests), decreasing the cost per m3 of water.

In the manufacturing industry, orders are typically scheduled and delivered through batches, and the probability of machine failure under high-load operation is high. On this basis, we focus on a single machine batch scheduling problem with a maintenance interval (SMBSP-MI). The studied problem is expressed by three-field representation as 1|B,MI|\sum{F_j+\mu}m, and the optimization objective is to minimize total flow time and delivery costs. Firstly, 1|B,MI|\sum{F_j+\mu}m is proved to be NP-hard by Turing reduction. Secondly, shortest processing time (SPT) order is shown the optimal scheduling of SMBSP-MI, and a dynamic programming algorithm based on SPT (DPA-SPT) with the time complexity of O(n^3T_1) is proposed. A small-scale example is designed to verify the feasibility of DPA-SPT. Finally, DPA-SPT is approximated to a fully-polynomial dynamic programming approximation algorithm based on SPT (FDPAA-SPT) by intervals partitioning technique. The proposed FDPAA-SPT runs in O(\frac{n^5}{\varepsilon^2})\ time with the approximation (1+\varepsilon).

The calculation and visualization of the dose field in the decommissioning of nuclear facilities is one of the important functions of the decommissioning virtual simulation system, which can provide accurate and intuitive radiation field distribution and play an important role in determining the demolition plan and protecting personnel during the engineering implementation process. This article investigates the development of dose field calculation and visualization in the reactor decommissioning virtual simulation systems, and preliminarily proposes a technical plan suitable for the development of the decommissioning dose field calculation and visualization display programs for the first Heavy Water Research Reactor (HWRR) in China. Besides, the applicability of the selected scheme is analyzed, the functional requirement and development direction of the HWRR reactor decommissioning dose field calculation and visual display module are preliminarily determined. Furthermore, the reactor vessel of HWRR reactor is modeled and the dose field distribution was calculated and visualized. This research can provide technical support for the development of the decommissioning simulation system for the first HWRR reactor in China.

The container relocation problem (CRP) is one of the important topics in container terminals, which aims to find the optimal sequence of movements to minimize the total number of relocations needed to retrieve all the containers within a bay according to a given specific layout and retrieval order. However, determining such an order may be difficult due to uncertainty. Various CRP solution methods proposed so far show a common pitfall concerning the complexity of the solving process, whose scale grows exponentially with the number of containers. Considering the retrieval operation process of the inbound containers in a bay consists of several operation rounds, this paper focuses on the real-time operation scenario of each round to minimize the actual number of relocations during the pick-up process, i.e., starting from the initial layout of the bay, we minimize the sum of the incremental number of blocking containers due to the layout change and the number of reallocated containers unrelated to the target container. We present a heuristic algorithm (called SPFH) based on the three rules (LL, MSS, and FSS), considering the adjustment of the pick-up order of containers. Numerical experiments on a set of instances generated from the literature are performed. The proposed methodology excels in both execution efficiency and solution quality and is capable of solving instances that were impossible to solve before. This also makes our methods appealing for being applied in practice. The source codes are available at https://github.com/zhou-sf/rt-scrp.

Concrete is more stable than filler as a false roof in a downward approach type infill quarry, but the mechanical model of a concrete false roof is not clear. As a result, there has been limited application. Therefore, it is necessary to establish the mechanical model of a concrete artificial false roof to enhance the stability of the downward approach infill quarry. This paper combines theoretical analysis, model derivation, finite element analysis and field application methods. Firstly, the basic assumptions of thin plate theory and elasticity mechanics are used as the premise to establish the differential equations of the deflection curve of the concrete thin plate false roof. Then, the three types of bending moment generation of the concrete thin plate false roof are explored. Secondly, the relationship between the location of dangerous surface and dangerous point in hard-supported weak-slab structure and soft-supported weak-slab structure is analyzed, and the type of uniform load and calculation model of concrete thin-slab false roof are improved. Finally, according to the established mechanical model of thin slab false roof, finite element analysis and field application were carried out. The results show that there are three types of deflection curve differential equations in the concrete thin slab false roof perpendicular to the strike, and three extreme points exist in the middle section of the thin slab and on both sides of the surrounding rock. The hazardous surface of the hard-supported weak-slab structure is present at the intersection of the encircling rock and the thin-slab fake ceiling, with the perilous point situated on the top of this surface. The hazardous surface of the soft-supported weak-slab structure can be found in the centre section of the thin-slab fake ceiling, with the perilous point being located at the bottom of this surface. Moreover, the damage mode for both structures is that of maximum tensile stress damage. Upon conducting a finite element analysis, the results indicate that the concrete's maximum tensile stress is significantly high.

This work (in two parts) will review the recently developed predictive modeling methodology called “4th-BERRU-PM” and its applicability to energy systems as exemplified by an illustrative application to the Polyethylene-Reflected Plutonium (acronym: PERP) OECD/NEA reactor physics benchmark. The acronym 4th-BERRU-PM designates the “Fourth-Order Best-Estimate Results with Reduced Uncertainties Predictive Modeling” methodology, which yields best-estimate results with reduced uncertainties for the first fourth-order moments (mean values, covariance, skewness, and kurtosis) of the optimally predicted posterior distribution of model results and calibrated model parameters. The 4th-BERRU-PM uses the Maximum Entropy (MaxEnt) principle to incorporate fourth-order experimental and computational information, including fourth (and higher) order sensitivities of computed model responses to model parameters, thus incorporating, as particular cases, the results previously predicted by the second-order predictive modeling methodology 2nd-BERRU-PM, and vastly generalizing the results produced by extant data assimilation and data adjustment procedures. The 4th-BERRU-PM methodology encompasses the scopes of high-order sensitivity analysis (SA), uncertainty quantification (UQ), data assimilation (DA) and model calibration (MC). The application of the 4th-BERRU-PM methodology to energy systems is illustrated by means of the above-mentioned OECD/NEA reactor physics benchmark, which is modeled using the neutron transport Boltzmann equation involving 21976 imprecisely known parameters, the solution of which is representative of “large-scale computations.” The model result (“response”) of interest is the leakage of neutrons through the outer surface of this spherical benchmark, which can be computed numerically and measured experimentally. Part 1 of this work illustrates the impact of high-order sensitivities, in conjunction with parameter standard deviations of various magnitudes, on the determination of the expected value and variance of the computed response in terms of the first four moments of the distribution of the uncertain model parameters. Part 2 of this work will illustrate the capabilities of the 4th-BERRU-PM methodology for combining computational and experimental information, up to and including forth-order sensitivities and distributional moments, for producing best-estimate values for the predicted responses and model parameters while reducing their accompanying uncertainties.

The sexual reproduction of ascomycetes is controlled by the mating-type(MAT) locus. (Pseudo)homothallic reproduction has been hypothesized based on genetic data from Hirsutella sinensis (Genotype #1 of Ophiocordyceps sinensis). In this study, the differential occurrence of the mating-type genes in the MAT1-1 and MAT1-2 idiomorphs, which constitute the genetic control of O. sinensis reproduction, was analyzed in 237 strains and 5 genomes of H. sinensis. The mating-type genes were differentially transcribed in H. sinensis Strain L0106, and the MAT1-2-1 transcript in H. sinensis Strain 1229 was alternatively spliced, with an unspliced intron I containing stop codons; these findings constitute O. sinensis reproduction controls at the transcriptional and coupled transcriptional-translational levels. The occurrence of the mating-type genes and a-/α-pheromone receptor genes differed among the 5 H. sinensis genomes. The differential occurrence and transcription of these genes are inconsistent with self-fertilization under (pseudo)homothallism but suggest self-sterility of H. sinensis, which therefore needs a mating partner to achieve sexual outcrossing under heterothallism or hybridization. Consistent occurrence and transcription of the mating-type genes of both the MAT1-1 and MAT1-2 idiomorphs have been reported in natural and cultivated Cordyceps sinensis, but these findings are insufficient to determine the reproduction mode(s) because of the co-occurrence of >90 fungal species and multiple genomically independent genotypes of O. sinensis. The mutant MAT1-1-1 and α-pheromone receptor transcripts in natural C. sinensis result in the N-terminus or middle-truncated proteins, with changes in hydrophobicity and secondary structures of the proteins, suggesting heterogeneous fungal source(s) of the proteins and hybridization reproduction.

An active power dispatch method for a microgrid (MG) with multi-type loads, renewable energy sources (RESs) and distributed energy storage devices (DESDs) is the focus of this paper. The MG operates in a grid-connected model, and distributed power sources contribute to the service for load demands. The outputs of multiple DESDs are controlled to optimize the active power dispatch. Our goal with optimization is to reduce the economic cost under time-of-use (TOU) price, and to adjust the excessively high or low load rate of distributed transformers (DTs) caused by the peak-valley demand and load uncertainties. To simulate a practical environment, the stochastic characteristics of multi-type loads are formulated. The transition matrix of system state is provided. Then, a finite-horizon Markov decision process (FHMDP) model is established to describe the dispatch optimization problem. A learning-based technique is adopted to search the optimal joint control policy of multiple DESDs. Finally, simulation experiments are performed to validate the effectiveness of the proposed method, and the fuzzification analysis of the method is presented.

Nanocrystalline silicon oxide (nc-SiOx:H) is a multipurpose material with its varied application in solar cells as a transparent front contact, intermediate reflector, back reflector layer and even tunnel layer for passivating contacts, owing to easy tailoring of its optical properties. In this work, we systematically investigate the influence of gas mixture (SiH4, CO2, PH3 and H2), RF power and process pressure on the optical, structural and passivation properties of thin n-type nc-SiOx:H films prepared in an industrial, high throughput, plasma-enhanced chemical vapor deposition (PECVD) reactor. We provide a detailed description of the n-type nc-SiOx:H material develop-ment using various characterization techniques (SEM, EDX, Raman spectroscopy, and spectro-scopic ellipsometry) with a focus on the relationship between the material properties and the pas-sivation they provide to n-type c-Si wafers characterized by their effective carrier lifetime (τeff). Further, we also outline the parameters to be kept in mind while developing different n-type nc-SiOx:H layers for different solar cell applications. We report a tunable optical gap (1.8 – 2.3 eV) for our n-type nc-SiOx:H films as well as excellent passivation properties with τeff up to 3.5 ms (iVoc ~ 715 mV) before annealing. Oxygen content plays an important role in determining the crystallinity and hence passivation quality of the deposited nanocrystalline silicon oxide films.

Motivated by empirical observations, we propose a possible extension of Gibrat's law. By applying it into the random growth theory of income distribution (Gabaix, 2009), we find that the income distribution is described by a generalized Pareto-type distribution (GPD) with three parameters. We observe that there is a parameter /elta in the GPD that plays a key role in determining the shape of income distribution. By using the Kolmogorov-Smirnov test, we empirically show that, for typical market-economy countries, /elta is close to 0 significantly, such that the income distribution is characterized by a two-class pattern in which the bottom 90% of the population is approximated by an exponential distribution and the richest 1%~3% is approximated by an asymptotic power law. However, we empirically find that, for China both in planned economy period and in early stages of market reformation (from 1978 to 1990), /elta is significantly deviated from 0, such that the bottom of the population no longer conforms to an exponential distribution.

Spinocerebellar ataxia type 3 is a neurodegenerative disease caused by an abnormal expansion of CAG repeats in the disease gene ATXN3, leading to prolonged polyglutamine (polyQ) tracts in the respective protein ataxin-3. The polyQ-expanded protein forms polyQ-containing aggregates in several brain regions which leads to neuronal cell loss. Autophagy is thought to play a significant role in clearing these polyQ-containing protein aggregates. Therefore, the autophagy mechanism was investigated in SCA3 with focus on the autophagic protein p62/sequestosome-1. Human and mouse RNA sequencing data were analyzed to determine RNA expression level of autophagic genes. Additionally, autophagy was studied in the SCA3 304Q knock-in mouse model and through several cell culture experiments using HEK-293T cells transfected with ATXN3 of differ-ent polyQ lengths. For cell culture experiments, autophagy was induced with rapamycin treat-ment, proteasomal degradation was blocked with MG132 and degradation of autophagic pro-teins was investigated using cycloheximide treatment. Here, we report an increased autophagy impairment with SCA3 disease progression seen in HEK-293T cells transfected with ATXN3 plasmid with expanded polyQ tracts, higher aged 304Q-knock-in mice, or human post-mortem brain material. Furthermore, co-localized aggregation of the autophagic protein p62 and the dis-ease protein ATXN3 was observed in the course of SCA3 disease. Therefore, we suggest a signifi-cant role for p62 in aggregate formation seen in the neurodegenerative disease SCA3.

Existing research indicates that mineralization of Carlin-type gold deposit is related to geological structures, but current studies mostly focus on the qualitative association, and lack quantitative analysis of the relationship between mineralization and structural features, which limits the potential for predictive mineral exploration. In this study, the Nibao Carlin-type gold deposit was selected as the research area to conduct three-dimensional geological modeling, spatial analysis, and three-dimensional prospectivity modeling. Spatial analysis revealed that the majority of gold ore bodies are situated near the main fault, with gold mineralization primarily occurring within a spatial range of approximately -80 meters to 20 meters from the main fault plane. This spatial distribution predominantly aligns in a SW-NE direction. Spatial characteristic extraction of fault slope indicators suggests that the slope angles are conducive to the migration and accumulation of ore-forming hydrothermal fluids, with ore body metal content rapidly increasing within an angle range of approximately 20° to 60°. The shape analysis of the mainfault indicates that the principal spaces of mineralization enrichment are within -10m to 20m of the fault plane undulations, where gold resources are concentrated in a relatively flat area. However, the degree of mineralization is more pronounced in the protruding sections than in the depressed areas. The areas with significant dip changes in faults display higher variations in orebody attitudes, with mineralization noticeably weaker in areas where the fault steepness changes markedly. Ore-bearing units exhibit significantly higher grades within the -17° to 3° interval of fault steepness transition. This interval also sees a rapid accumulation of gold resources. The Nibao gold mine's mineralization is derived from deep, ore-bearing hydrothermal fluids ascending along major fractures, precipitating, and accumulating in favorable ore-hosting spaces (faults, anticline cores) and lithological combinations (sedimentary tuffs, tuffs). Three deep potential gold targets were identified, highlighting the deep western edge of the F1 fault and the deep southeast-trending lower plate as prospective areas for future exploration. This study underscores the effectiveness of 3D prospective modeling as an indispensable tool for elucidating the spatial distribution of fault-controlled Carlin-type gold deposits and their tectonic determinants.

In this paper, we have established Hermite-Hadamard inequalities for r-preinvex functions via fractional integrals.

Background and Objectives Neurofibromatosis 1 (NF1 MIM # 162200), also known as von Recklinghausen disease, is among the most common autosomal dominantly transmitted disorders characterized mainly by neurocutaneous manifestations such as café au lait spots, intertriginous freckling, various types of neurofibromas (cutaneous neurofibromas or plexiform tumors), neurological manifestations, and bone abnormalities. It occurs with an incidence of 1:3000–1:4000 cases. Cone-rod dystrophies belong to the large group of retinal dystrophies, a highly heterogeneous group of disorders clinically manifested mainly by damage to cone and rod cells and have a broad spectrum of clinical manifestations. Many genes are involved in their etiology, with more than 100 currently identified, including the CRX gene responsible for the autosomal dominant form of cone-rod dystrophy (ad CORD). Materials and Methods: The authors present an intriguing case of a patient diagnosed with Neurofibromatosis type 1 (NF1) as an infant and ocular involvement (myopia and astigmatism onset at age 15), which yielded a surprising molecular testing result. Results: Molecular DNA analysis identified a rare occurrence of two mutational variants, including a pathogenic variant in the NF1 gene, c.3871-2A, and a likely pathogenic variant in the CRX gene c.119G>A. Conclusions: The authors present this case to highlight the surprises offered by molecular testing in a condition where the diagnosis is usually based on clinical criteria, thus providing patients with competent genetic advice.

In this paper, we establish the generalized Qi-type inequality involving conformable fractional integrals. The results presented here would provide extensions of those given in earlier works.

Aiming to reveal the kinetic characteristics of ceramic ball grinding of fine magnetite comprehensively, two types of ceramic balls ground with the same filling rate and total weight as steel balls were researched. The results show that the breakage rate of ceramic ball grinding is only half of that of steel ball grinding with the same media filling rate. With the same total media weight and the feed size less than 0.212 mm, the breakage rate of the ceramic ball grinding approaches the steel ball grinding and is 21.43% higher than that of the steel ball grinding. The main crushing form of magnetite changed from impact to abrasion in ceramic ball grinding compared with steel ball grinding, which significantly affected the value of the zero-order output constant a. Consequently, there is a noticeable difference in the variation of the parameter β related to the fines generation rate of the ceramic ball grinding compared with the steel ball grinding.

This paper is devoted to the study of multi-dimensional integral transform with Fox H-function in the kernel in weighted spaces integrable functions in the domain R+n with positive coordinates. Mapping properties such as the boundedness, the range, the representations of the considered transformation are established.

We present a case of a 47-year-old male who died unexpectedly from acute pulmonary hemorrhage 555 days after completing the BNT162b2 (Pfizer) COVID-19 vaccination primary series. Before death, he exhibited symptoms of a mild respiratory infection. Despite a healthy medical history and no medication use, the patient’s condition rapidly deteriorated and he experienced severe respiratory distress, followed by cardiopulmonary arrest with evidence of profuse pulmonary bleeding. Autopsy findings revealed massive lung congestion without embolism, normal heart size, moderate coronary atherosclerosis without myocardial infarction, and no evidence of other hemorrhagic events. The patient tested negative for COVID-19 and other respiratory pathogens at autopsy. Despite these findings, the medical examiner determined the cause of death was attributed to atherosclerotic and hypertensive cardiovascular disease, without considering the recent pulmonary hemorrhage and unremarkable medical history. Investigation into the vaccine batch indicated a higher-than-average number of serious adverse events, including fatalities. The patient's BNT162b2 batch was among the top 2.8% for reported deaths. Moreover, the autopsy failed to investigate potential contributions from the vaccine, such as the presence of the Spike protein or related antibodies. The evidence suggests that the pulmonary hemorrhage, exacerbated by a viral infection, was the immediate cause of death, with the COVID-19 vaccine potentially playing a role in the development of cardiopulmonary pathology and hemorrhage. We propose autopsy protocols for COVID-19 vaccine recipients to better investigate vaccine-related pathologies among those with one or more prior injections.

To assess the community health workers’ (CHWs) knowledge, attitude, and practices (KAP) in type-2 diabetes (T2D) and hypertension (HTN) management. We thus also sought to assess the potential health system barriers for incorporating CHWs in T2D and HTN management. Mixed-method study comprised (1) a survey among 153 active CHWs to assess their KAP of T2D and HTN, and (2) semi-structured interviews with key informants to understand challenges of the health system and proposed solutions in incorporating CHWs in T2D and HTN management. The majority of CHWs correctly answered general knowledge questions on NCDs, risk factors, and prevention; however, only one-quarter correctly answered questions on smoking, family history, or tobacco use as risk factors. Most respondents appeared to have positive attitudes and have been practicing some activities related to T2D and HTN. Both financial and non-financial resource constraints were cited as challenges of the health system; therefore, re-structuring the role definition of CHWs and task of responsibilities, and assessment the need and workload have been proposed as ways forwards to effectively incorporate CHWs in T2D and HTN care. CHWs in Cambodia have shown their potential in T2D and HTN management; however, a well-designed strategy including technical training, clearly defined roles and responsibilities, and strong supporting structure are important to maximize their potential in the health system.

This paper investigates the importance of enhancing the safety of research reactors, especially in newcomer countries where experience may be limited. The focus is on the role of Independent Assessment (IA) and assessors in addition to using Best Estimate plus Uncertainty (BEPU) approach in enhancing safety measures. By using the best estimate codes to perform uncertainty calculations, the BEPU technique logically connects nuclear thermal hydraulics and nuclear reactor safety. BEPU is based on an understanding of the phenomena related to the reactor state. Reactor circumstances, such as those related to operations or accidents, could be identified in this way. The IA process, involves experts supporting design, installation, safety analysis, decommissioning, and modification. In order to shape the use of IA process in this study feedback from nuclear professionals was collected through questionnaires, revealing a consensus on the necessity of qualified regulatory bodies in newcomer countries. Additionally, the questionnaires highlighted the potential benefits of IA, such as minimizing accident risks and providing valuable insights for future analyses. The results indicate a global anticipation of increased research reactor construction, particularly in newcomer countries. The study discusses the propagation of input uncertainties, emphasizing the importance of considering various factors and parameters in safety assessments. The BEPU method was applied by using the COBRA-TF code by modeling onset of nucleate boiling (ONB) conditions for VVR-K research reactor. For uncertain parameters; ±1% inlet/initial pressure, ±1% inlet temperature, and ±1% linear heat rate, the ONB temperature in the axial region with the average cladding temperature was calculated to be 114.44℃. Table 2 (our references) provided a value of 114.6℃. The close agreement between the reference value and the COBRA-TF result shows that the model used in the calculation of the reference value and the Chen relation used by COBRA-TF are compatible.

Currently, most plastic waste stems from packaging materials, with a large proportion of this waste either discarded or used to derive fuel. Accordingly, there is growing interest in the use of pyrolysis in the context of chemically recycling non-recyclable (i.e., via mechanical means) plastic waste into petrochemical feedstock. The aim of this study is to use kinetic analyses to elucidate the pyrolytic properties of solid recovered fuel (SRF) produced using two types of reactors, namely, fixed bed and fluidized bed reactors. From the kinetic analysis, the activation energy corresponding to the rate of pyrolysis reaction conversion was obtained. In conclusion, we found that the pyrolysis occurs more vigorously in fluidized bed reactors compared to fixed bed reactors.

Industrial pilot projects often rely on proprietary and expensive electronic hardware to control and monitor experiments. This raises costs and retards innovation. Open-source hardware tools exist for implementing these processes individually, however, they are not easily integrated with other designs. The Broadly Reconfigurable and Expandable Automation Device (BREAD) framework provides many open-source devices which can be connected to create more complex data acquisition and control systems. This article explores the feasibility of using BREAD plug-and-play open hardware to quickly design and test monitoring and control electronics for an industrial materials processing prototype pyrolysis reactor. Generally, pilot-scale pyrolysis plants are expensive custom designed systems. The plug-and-play prototype approach is first tested by connecting it to the pyrolysis reactor and ensuring it can measure temperature and actuate heaters and a stirring motor. Next, a single circuit board system was created and tested using the designs from the BREAD prototype to reduce the number of microcontrollers required. Both open-source control systems were capable of reliably running the pyrolysis reactor continuously and the overall cost of control was reduced by more than 10X. Open-source, plug-and-play hardware provides a reliable avenue for researchers to quickly develop data acquisition and control electronics for industrial-scale experiments.

To achieve efficient utilization of low-concentration mine gas, reduce resource waste, and alleviate environmental pollution, high-temperature oxidation of low-concentration gas at a concentration range of 1.00% to 1.50% that is directly discharged into the atmosphere during coal mine production was oxidized to recover heat for reuse. The gas oxidation equipment was improved for the heating process, and the safety of low-concentration gas oxidation under high-temperature environment was evaluated. Experimental results showed that the reactor could provide a 1000 ℃ high-temperature oxidation environment for gas oxidation after installing high-temperature resistant ceramics. The pressure variation curves of the reactor with air and different concentrations of gas were similar. Due to the thermal expansion, the air pressure slightly increased and then returned to normal pressure. In contrast, the low-concentration gas exhibited a stable pressure response in the high-temperature environment of 1000 ℃. The outlet pressure was significantly greater than the inlet pressure, and the pressure difference between the inlet and outlet exhibited a trend to increase with the gas concentration. The explosion limit varied with the temperature and the blend with oxidation products. The ratio of measured gas pressure to air pressure after oxidation was below the explosion criterion, indicating that the measured concentration gas is still safe after the shift of explosion limit, which provides a safe concentration range for efficient use of low-concentration gas in the future.

This paper presents the results of investigations on the pyrolysis of tyres waste in a laboratory fixed bed batch reactor. The results regarding the influence of either the reaction temperature (425, 450, 475, and 500 °C) and the flow of the inert gas (0, 100, 300, and 500 mL/min) on products yield (referred to pyrolysis of waste tyres) are also considered and discussed. On the basis of the above mentioned findings, the most appropriate experimental conditions were selected to contribute to a higher yield of pyrolysis oil. The sample of pyrolysis oil obtained from the experiments carried out in the selected optimal conditions (reaction time 120 min, temperature 450 °C and the inert gas flow of 100 mL/min) was subjected to a calorimetric and infrared spectroscopy analysis. The results of IR spectroscopy analysis on this oil sample showed the following content in percentages (by mass): 32.59 % of aromatics, 51.06 % of paraffins and 16.35 % of naphthenes. The pyrolysis oil so obtained has a high calorific value (42 MJ/kg) and a low sulfur content (0.41 % by mass), which makes it an excellent raw material for energy production. The solid product, i.e. pyrolysis char or carbon black, has the potential to be used as an adsorbent or catalyst support after an activation process, which changes its irregular pore structure to a more voluminous one, making the overall structure more crystalline and symmetrical. Pyrolysis coal has a fairly high calorific value of 31 MJ/kg, when compared to typical solid fuels, so it can be used in energy production or as a feedstock for the gasification process.

A nuclear reactor leak is undesirable and unpredictable occurrence in the plant of nuclear energy generation. For this reason, an accurate equipment is needed to inspect and supervise reactors. There are two kind of reactors for research and industry reactors, both of them are needed to inspect. Concern to inspection, regularly check is needed to ensure reactor conditions are always safe to operate. For that reason, an application of advanced technology for detecting leaks is priority. In this research, we are using underwater robot to be implemented. Using the robot is able to detect periodically, continuously with accurate reports to the operator or reactor supervisor. Furthermore, Underwater Robot namely Robaru, with 4 propellers equipped at the robot, Robaru can move on 3 axes x, y and z and rotate. The use of cameras, as well as temperature measurements to obtain data when conducting inspections of the reactor. The survey method used is direct observation to detect leaks. The robot is at a certain distance by following a predetermined connection or path. The robot has successfully performed a good check with an accurate level of positioning accuracy.

The three-pronged sliding universal coupling is a new kind of three-pronged coupling. To study its kinematic and dynamic characteristics, directional cosine matrices are utilized as tools to analyze the coordinate systems by establishing simplified geometric models and corresponding motions. Through the analysis of the motions of the input and output shafts and employing the method of single-force element, a set of equilibrium equations for the forces acting on the output shaft and the input shaft are formulated for solution analysis. The research indicates that during the rotation process, there exists a small angular difference between the input shaft and the intermediate shaft, demonstrating the quasi-constant angular velocity characteristics of the new tripod sliding universal coupling. The curves of the forces and torques acting on each component of the coupling approximate sinusoidal curves. The optimal operating angle range for the new tripod universal coupling is at angle , during which the system exhibits good transmission and mechanical performance.

Protein tyrosine phosphatase receptor type E (PTPRE) is a member of the “classical” protein tyrosine phosphatase subfamily and regulates a variety of cellular processes in a tissue-specific manner by antagonizing the function of protein tyrosine kinases. PTPRE plays a tumorigenic role in different human cancer cells, but its role in retinoblastoma (RB), the most common malignant eye cancer in children, remains to be elucidated. Etoposide resistant RB cell lines and RB patients display significant higher PTPRE expression levels compared to chemosensitive counterparts and the healthy human retina, respectively. PTPRE promotor methylation analyses revealed that PTPRE expression in RB is not regulated via this mechanism. Lentiviral PTPRE knockdown (KD) induced a significant decrease in growth kinetics, cell viability, and anchorage independent growth of etoposide resistant Y79 and WERI RB cells. Caspase-dependent apoptosis rates were significantly increased and a re-sensitization for etoposide could be observed after PTPRE depletion. In vivo chicken chorioallantoic membrane (CAM) assays revealed decreased tumor formation capacity as well as reduced tumor size and weight following PTPRE KD. Expression levels of miR631 were significantly downregulated in etoposide resistant RB cells and patients. Transient miR631 overexpression resulted in significantly decreased PTPRE levels and concomitantly decreased proliferation and increased apoptosis levels in etoposide resistant RB cells, impacts mirroring PTPRE KD effects and indicating PTPRE regulation via this miR. Additionally, PTPRE KD led to altered phosphorylation of protein kinase SGK3 and - dependent on the cell line - also AKT and ERK1/2, suggesting potential PTPRE downstream signaling pathways. In summary, these results indicate an oncogenic role of PTPRE in chemoresistant retinoblastoma.

Four strains of green microalgae (Scenedesmus acutus, Scenedesmus vacuolatus, Chlorella sorokiniana and Chlamydomonas reinhardtii) were compared for growth and pigment composition under photoautotrophic or heterotrophic conditions. Batch growth experiments were performed in multicultivators with online monitoring of optical density. For photoautotrophic growth, light-limited (CO2-sufficient) growth was analysed under different light intensities during exponential and deceleration growth phases. The specific growth rate, mesured during exponential phase, and the maximal biomass productivity, measured during deceleration phase, were not related to each other when different light intensities and different species were considered. This indicates species-dependent photoacclimation effects during cultivation time, which was confirmed by light-dependent changes in pigment contents and compositions when exponential and deceleration phases were compared. Except for C. reinhardtii, that does not grow on glucose, heterotrophic growth was promoted to similar extents by acetate or by glucose, however these two substrates led to different pigment compositions. Weak light increased pigment contents during heterotrophy in the four species, but was efficient in promoting growth only in S. acutus. C. sorokiniana and S. vacuolatus exhibited the best potential for heterotrophic biomass productivities both on glucose and acetate, with carotenoid (lutein) content being the highest in the former.

In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance of the selected cell factory in larger reactors, has a major influence on the performance of the final process. To overcome this, scaledown approaches are essential to run screenings that show the real cell factory performance at industrial like conditions. We present a fully automated robotic facility with 24 parallel mini-bioreactors that is operated by a model based adaptive input design framework for the characterization of clone libraries under scale-down conditions. The cultivation operation strategies are computed and continuously refined based on a macro-kinetic growth model that is continuously re-fitted to the available experimental data. The added value of the approach is demonstrated with 24 parallel fed-batch cultivations in a mini-bioreactor system with eight different Escherichia coli strains in triplicate. The 24 fed-batches ran under the desired conditions generating sufficient information to define the fastest growing strain in an environment with varying glucose concentrations similar to industrial scale bioreactors.

Background and Objectives: In this current state, Type 2 Diabetes Mellitus (T2DM) is more prevalent in the population, and metformin is used as a first-line medication for treating it. There was up surge in the utilization of alternative therapies in the management of diabetes. Especially in diabetes, Herbal medicines are considered safe and reliable by majority of the population. Aim: The purpose of this research is aimed to estimate the safety and efficacy of Polyherbal Metabolite Compounds (PHMC) capsules in adults with T2DM. Materials and Methods: In this study, 60 T2DM patients aged 18-60 years were randomly assigned to Groups A (30) receiving Metformin 500 mg twice a day after food and Group B (30) receiving PHMC capsule 500 mg twice a day after food in a prospective, randomized and open-label clinical study. The in-silico simulation study was performed to selected plants major compounds on target protein Insulin-like Growth Factor-1 (1K3A). Result: The PHMC is found to be safe and effective in the management of T2DM. The FBS, PPBS and HbA1c were significantly lowered (p

Biofilm serves as the residence for bacteria, wherein bacteria attach to surfaces and produce extracellular polymeric substances, including proteins, polysaccharides, and extracellular DNA. In recent years, biofilm reactors have gained increasing significance, playing a crucial role, for instance, in immobilizing heavy metals from waterways. This paper employs a bibliographic study to analyze the most important keywords and organizations in the field of biofilm reactors, identifying recent significant papers. Additionally, the paper discusses the opportunities for the future development of biofilm reactors. Through this study, a better understanding of key factors in biofilm reactor research is achieved, providing valuable guidance for future studies and applications in this field.

Background and Objectives: Cancer as the second leading cause of death in the United States poses a huge healthcare burden. Barriers to access to advanced therapies influence the outcome of cancer treatment. In this study, we examined whether insurance types affect the quality of cancer clinical care regarding access to an important monoclonal antibody, ramucirumab. Materials and Methods: Data for 13,340 cancer patients with Purchased or Medicaid insurance from the All of Us Database were collected for this study. The Chi-square test of proportions was employed to determine the significance of patient cohort characteristics and ramucirumab usage between Purchased and Medicaid insurance groups. The independent t-test was utilized to assess the influences of other determinants on insurance types. Results: Cancer patients who are African American, with lower socioeconomic status, or with lower educational attainment are more likely to be insured by Medicaid. Analysis of survey questions demonstrated the relationship between income and education level with insurance type as Medicaid cancer patients were less likely to receive primary care and specialist physician access and more likely to request lower cost medications. In addition, those with Medicaid insurance were identified to have poorer access to expensive therapeutics like ramucirumab compared to those with Purchased insurance.  Conclusions: The inequities of the US healthcare system are observed for cancer patient care; access to physicians and therapeutics are highly varied and dependent on insurance types. Socioeconomic status determines insurance type, which unfortunately generates a significant impact on cancer treatment and disease outcome.

Nowadays, there has been a significant rise in the consumption of kefir, a functional beverage touted for its perceived health benefits. To offer a high-quality beverage to consumers, it is imper-ative to scrutinize and fine-tune the fermentation process. This study seeks to investigate the im-pact of fermentation time and the number of subcultures on the physicochemical, microbiological, and volatile composition, as well as the visual appearance of kefir beverages obtained from four consecutive 24- or 48-h batch subcultures. All fermented beverages exhibited low lactose, ethanol and acids levels, with counts of viable probiotic lactic acid bacteria and yeast exceeding 106 colony forming units/mL. The four kefir beverages from the 48-h batch subcultures notably showed the lowest total concentrations of volatile compounds likely due to overfermentation and overacidifi-cation of the beverages. This caused separation of the whey and curd, along with the formation of large gas bubbles, negatively affecting the visual appearance of the products. These findings em-phasize the importance of fine-tuning the fermentation process to ensure the production of high-quality kefir beverages that align with consumer preferences. The four beverages from the 24-h batch subcultures exhibited high microbiological and physicochemical stability during stor-age at 4 °C for 28 days.

Calcium and magnesium are important not only for human health, but also for reducing problems related to the corrosive and aggressive effects of soft water on drinking water distribution materials. Experimental and mathematical modeling of the recarbonization process aimed at increasing the content of these biogenic elements in water was carried out using the novelty of a continuous laboratory and pilot scale fluidized bed reactors. Water remineralization using half-calcined dolomite (HCD) and carbon dioxide was used. The influence of operating conditions Q(CO2), freshwater inflow and HCD dose on quality indicators of treated drinking water (c(Ca2+), c(Mg2+), c(Ca2++Mg2+) and Ca/Mg) was studied. The results show that the concentration of Mg2+ is more significantly affected by the amount of HCD in the system and the flow of CO2. The influence of freshwater inflow on the Mg2+content is to a lesser extent. At a constant CO2 flow, therefore, as the tap water inflow increases, the Ca2+ content decreases and the Mg2+ content increases, which has results in a decrease in the Ca/Mg molar ratio. However, the Ca/Mg ratio can be effectively controlled by adding an appropriate amount of HCD at certain time intervals. Overproduction of ions is easily controlled by CO2 flow.

During the long-term operation of the plasma reactor, decreases in the plasma concentration were noticed despite the constant maintenance of all parameters. One of the factors is the decrease of the nitrogen content on the surface of the electrode, in order to eliminate it, the supply voltage has been increased to 11 kV. The next decisive factor in the decrease of plasma concentration is the oxidation of the electrode surface, therefore two electrodes were used: first one with solid gold and the other one copper covered with galvanized gold with a thickness of 10 μm. During the experiment, a large decrease in plasma concentration was observed when the electrode coated by gold was used. High-energy electrons have knocked out the gold atoms from the electrode, as a result of which the gold evaporated and the raids layers formed. After a month of working of the electrodes, metallographic researches were carried out, the results of which are described in this publication.

After a long a glorious history, marked by the first direct proofs of neutrino existence and of the mixing between the first and third neutrino generations, the reactor antineutrino experiments are still well alive and will continue to give important contributions to the development of elementary particle physics and astrophysics. In parallel to the SBL experiments, that will be dedicated mainly to the search for sterile neutrinos, a new kind of experiments will start playing an important role: the medium baseline reactor experiments, aiming to study the neutrino mass ordering. The first example of this kind, the liquid scintillator JUNO experiment, characterized by a very high mass and unprecedented energy resolution, will soon start data taking in China. Its main aspects are discussed here, together with its potentialities for what concerns the mass ordering investigation and also the other issues that can be studied with this detector, spanning from the accurate oscillation parameter determination, to the study of solar neutrinos, geoneutrinos, atmospheric neutrinos and neutrinos emitted by supernovas and to the search for signals of potential Lorentz invariance violation.

The process of sexual reproduction in eukaryotes starts when gametes from two different sexes encounter each other. Paramecium, a unicellular eukaryote, undergoes conjugation and uses a gametic nucleus to enter the sexual reproductive process. The molecules responsible for recognizing mating partners, hypothetically called mating-type substances, are still unclear. We have identified an O3-type mating substance polypeptide and its gene sequence using protein chemistry, molecular genetics, immunofluorescence, RNA inter-ference, and microinjection. The O3-type substance is a polypeptide found in the ciliary membranes, located from the head to the ventral side of cells. The O3-type substance has a kinase-like domain in its N-terminal part located outside the cell and four EF-hand motifs that bind calcium ions in its C-terminal part located inside the cell. RNA interference and immunofluorescence revealed that this polypeptide positively correlated with the expression of mating reactivity. Microinjection of an expression vector incorporating the O3Pc-MSP gene induced additional O3 mating type in the recipient clones of different mating types or syngen. Phylogenetic analysis indicates this gene is widely present in eukaryotes and exhibits high homology among closely related species. The O3Pc-MSP gene had nine silent mutations compared to the complementary mating type of the E3 homologue gene.

(1) Background: Among the chronic complications of type 2 diabetes mellitus, cancer became the leading cause of death in several countries. Our objective was the determination of whether prevalent type 2 diabetes mellitus is associated with a higher incidence of cancer. (2) Methods: This study is a nationwide analysis performed in Hungary. Study population was divided into a group with type 2 diabetes mellitus and into a non-diabetic group. The primary outcome was the risk related to overall cancer incidence. A key secondary outcome was the overall incidence of cancer in distinct study years. Further outcomes were the annual percent changes. (3) Results: The odds ratio related to the overall incidence of cancer was 2.50 (95% confidence interval: 2.46-2.55, P<0.0001) in patients with diabetes as related to controls. The odds ratio was higher in males than in females. The highest risk was found in the 18-39-year-old subgroup. The annual cancer incidence rate declined in controls, but not in patients with diabetes. Several types of cancer showed a decreasing tendency in non-diabetic controls.; (4) Conclusions: type 2 diabetes is associated with a higher risk of cancer. High risk in young patients with type 2 diabetes suggests that these young individuals should be considered as a risk group.

(1) Background: Patients with type 2 diabetes mellitus (T2DM) are at higher risk of cancer but how these two diseases associate is still debated. The goal was the assessment of the overall incidence of cancer among patients with newly diagnosed T2DM in Hungary. Summarizing, incident T2DM is associated with a significantly higher overall risk of incident cancer, with a reverse correlation of age. Newly registered T2DM patients were suggested to be screened for cancer within 6 months. (2) Methods: A nationwide, retrospective, longitudinal study was performed using a Hungarian database. The incident T2DM and diabetes free Hungarian population were included in the study. The primary endpoints were the overall and site-specific incidence and annual percentage change of the incidence of cancer in both populations. (3) Results: The overall incidence of cancer in patients amounted to 29.4/1000 and 6.6/1000 with or without T2DM, respectively, and the OR (95%CI) of cancer of the T2DM group was 4.32 (4.14-4.53), p<0.0001. The risk of having cancer was age-dependent, having the highest odd in the age group 18-39. The incidence of cancer was declining in the non-diabetic but was unchanged in T2DM population. The average lag time of diagnosing a cancer after the detection of T2DM was 3.86 months.; (4) Conclusions: Iincident T2DM is associated with a significantly higher overall risk of incident cancer, with a reverse correlation of age. Newly registered T2DM patients were suggested to be screened for cancer within 6 months.

Diabetes mellitus (DM) poses a significant challenge to global health, with its prevalence projected to rise dramatically by 2045. This narrative review explores the bidirectional relationship between periodontitis (PD) and type 1 diabetes mellitus (T1DM), focusing on cellular and molecular mechanisms derived by the interplay between oral microbiota and the host immune response. A comprehensive search of literature published between 2008 and 2023 was conducted to elucidate the association between these two diseases. Preclinical and clinical evidence suggests a bidirectional relationship, with individuals with T1DM exhibiting heightened susceptibility to periodontitis, and vice versa. The review includes recent findings from human clinical studies, revealing variations in oral microbiota composition in T1DM patients, including increases in certain pathogenic species such as Porphyromonas gingivalis, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans, along with shifts in microbial diversity and abundance. Molecular mechanisms underlying this association involve oxidative stress and dysregulated host immune responses, mediated by inflammatory cytokines such as IL-6, IL-8, and MMPs. Furthermore, disruptions in bone turnover markers, such as RANKL and OPG, contribute to periodontal complications in T1DM patients. While preventive measures to manage periodontal complications in T1DM patients may improve overall health outcomes, further research is needed to understand the intricate interactions between oral microbiota, host response, periodontal disease, and systemic health in this population.

The vomeronasal system serves as a pivotal element in mammalian sensory biology, allowing the perception of chemical signals essential for social communication via the activation of two main receptor families, V1R and V2R. The expression of V1Rs and V2Rs is linked with that of the G-protein alpha-subunits, Gαi2 and Gαo, respectively. The exploration of the evolutionary pathways of V1Rs and V2Rs across all mammalian species remains a main challenge, juxtaposing available genomic data against emerging immunohistochemical evidence. However, recent investigations, in contrast to what is predicted by the currently annotated genomic sequences, have revealed the expression of Gαo in the vomeronasal neuroepithelium of wild canids, including wolves and foxes. In the present study, the employment of a specific antibody raised against the mouse V2R2, member of the C-family of vomeronasal receptors, V2Rs, has confirmed the expression of this receptor in the fox and wolf, but it has revealed the lack of expression in the dog. This may reflect the impact of domestication on the regression of the VNS in this species, in contrast to their wild counterparts, and underscores the effects of artificial selection on sensory functions. Thus, these findings suggest a more refined chemical detection capability in wild species.

Recently, SMR (Small Modular Reactor) is expected to be able to operate flexibly in conjunction with a high renewable energy penetration grid as a result of improved safety and easy power control compared to large nuclear power plants. SMRs such as South Korea’s System-integrated Modular Advanced ReacTor (SMART) are designed to use canned motors (CMs) for their reactor coolant pumps (RCPs) to enhance safety. The CMs passively enhance the reactor’s safety by preventing leakage of radioactive reactor coolant. However, with motor sizes designed to be as small as possible, and the increased air gap of CMs between stator and rotor, starting torque may be insufficiently small compared to that of similar sized induction motors (IMs). Thusly, CMs may require variable frequency drives (VFDs) to start. This paper compares the torque characteristics of CMs with those of IMs for SMART’s RCPs. ETAP is then used to perform motor starting analysis for CMs started with and without VFDs. The results are presented and analyzed to find out if the VFDs deal with the CM starting torque issue.

Ammonia known as harmful gas that could impact on health and environment. Typically, ammonia gas emitted by fertilizer industry, rubber factory, etc. There are many options for advance industry to control ammonia gas pollution, absorption tower using water scrubber and using activated carbon as adsorption reaction could be an option. But for middle- and lower-class industry, it is common in Indonesia, as seen on many rubber factories, ammonia gas pollution is serious problem to control, actually they have used wet scrubber but eficiency is lower about 47%. This study measure performance for ammonia elimination as gas pollutant using absorption tower and adsorption tower in laboratory scale. Using electrochemical gas sensor, we measure both ammonia concentration for inlet and outlet simultaneously to settle saturation point of these two types of ammonia emission control. In conclusion, Ammonia absorbed in water proportional to saturation time and absorbent volume. Highest absorbed ammonia concentration 1.538 mg/L on 4000 mL absorbent. For Adsorption system, saturation time proportional and correlated to adsorbent weight and Adsorption capacity reverse correlated to adsorbent weight. Optimum adsorption point can be achieved from intersection curve between saturation time and adsorption capacity which is 1200 grams adsorbent

Researchers from around the world are looking for better and cheaper ozone production. One of the methods increasing the efficiency of ozone production is the use to a rotating electrode presented in this paper. Experiments were carried out which shows that the most important parameters are the materials used on the electrodes and the condition of its surface. The metallographic investigations of the electrodes after continuous monthly work was made, which show how the raids layers are formed. As a result of working in a highly oxidizing environment, the electrode is oxidized in the process of chemical corrosion. It is obvious that the layer of corrosion products created during the work of the plasma reactor isolates the surface of the electrode, which reduces the intensity of the electric field, causing a decrease in the amount of plasma generated, which is a direct cause of lowering the concentration of ozone during this process. The dynamics of plasma generation process and the type of electrode material working in changing process conditions are the decisive factors influencing the concentration of ozone produced. The influence of the medium, which is the electrode material, depends mainly on its resistance to corrosion in the environment of dynamically changing conditions, e.g. electrode rotation, oxygen flow through the rotating electric field and the long monthly working time of the plasma reactor.

Cadmium(II) contamination in the environment is an emerging problem due to its acute toxicity and mobility, so it is very urgent to remove this species from industrial wastewater before it is discharged into the environment. Thus, a starch-based activated carbon (AC) with a specific surface area of 1600 m2g-1 is used as an adsorbent for the capturing of toxic cadmium(II) ions from synthetic solutions. The sorbent is characterized by BET, SEM, TEM, XRD, FT-IR, TGA, and zeta potential. The maximum uptake (284 mg g-1) of Cadmium(II) ion is obtained at pH 6. The thermodynamic parameters like ∆G, ∆H, ΔS are found to be -17.42 kJmol-1, 6.49 kJ mol-1, and 55.66 Jmol-1K-1 respectively, revealing that the adsorption mechanism is endothermic, spontaneous, and feasible. The experimental data follows the D-R and Langmuir models well. The mass transfer is controlled by pseudo 2nd order kinetics. Furthermore, the density functional theory simulations demonstrate that the activated carbon strongly interacted with the Cd(II) ion through its various active sites. The adsorption energy noted for all interactive sites is highly negative (-0.45 eV to -10.03 eV), which shows that the adsorption process is spontaneous and stable which is in agreement with the experimental thermodynamics analysis.

In the wake of the rapid deployment of Small Modular Reactors (SMRs), this study aims to enhance the efficiency, reliability, and safety of SMR operations through a deeper understanding of human factors in their interaction within digital control room systems. Recognizing the pivotal role of human understanding in this new era of nuclear power, we employed electroencephalogram (EEG)-based monitoring to provide an unparalleled real-time view into operators' cognitive states. By interfacing detailed human models, informed by EEG metrics, with specific operational tasks, we recreate potential operational scenarios using an SMR simulator and capture intricate human responses therein. Our results elucidated the intricate relationship between EEG-derived data and human performance shaping factors, indicating a marked correlation between certain EEG patterns and operational efficiencies. Conclusively, these findings underscore the potential of EEG monitoring not only as a diagnostic tool but as an instrumental aid in the design and operation of future SMR digital control rooms. The insights derived offer a roadmap for the development of practical strategies, ensuring more effective and safer SMR operations.

Soy leghemoglobin (LegH) has been gaining interest over the last years as an efficient flavor and aroma compound in plant-based meat substitutes. Hence, in the following article we demonstrate the methods for LegH production using a recombinant Komagataella phaffii strain. Multiple fed-batch fermentation with an alternative to BSM medium, where glucose was used as the main carbon source, were implemented and the growth kinetics, e.g., maximal specific biomass growth - 0.239 g·g−1·h−1, biomass yield from substrate - 0.298 g·g−1 and maximal specific substrate consumption rate - 0.81 g·g−1·h−1, were identified. Leghemoglobin production resulted in a yield of 0.513 mg·gDCW 1, while the highest biomass density achieved in the study was 121.80 gDCW·L 1. The applied medium showed potential for additional optimization studies, as in contrast to BSM, made it possible to separate pH control from nitrogen supply, does not affect medium turbidity measurements and does not induce metabolite synthesis during yeast biomass growth.

This study explored the feasibility of fluoride removal from simulated semiconductor industry wastewater and its recovery as calcium fluoride using fluidized bed crystallization. The continuous reactor showed best performance (>90% fluoride removal and >95% crystallization efficiency) at a calcium to fluoride ratio of 0.6 within first 40 days of continuous operation. The resulting particle size increased by more than double during this time along with a 36% increase in the seed bed height indicating deposition of CaF2 onto the silica seed. The SEM-EDX analysis showed the size and shape of the crystals formed along with high amount of Ca-F ions presence. The purity of the CaF2 crystals were determined to be 91.1% though ICP-OES analysis. Following the continuous experiment, different process improvement strategies are explored. The addition of excess amount of calcium resulted in an additional 6% fluoride removal, however, compared to this single stage process, a two-stage approach was found to be better strategy to achieve a low effluent fluoride concentration. The fluoride removal reached 94% with this two-stage approach under optimum condition of 4 + 1 h HRT combinations and a [Ca2+]/[F-] ratio of 0.55 and 0.7 for the two reactors, respectively. CFD simulation showed the impact of inlet diameter, bottom-angle shape and width and height ratio of the reactor on mixing inside reactor and possibility of further improvement in reactor performance by optimizing the FBR configuration.

The present study shows a methodology for analyzing and designing a cylindrical packed-bed reactor considering stationary and dynamic models. The design comprises the reactor's stationary and dynamic governing differential equations for mass and heat transfer under multi-dimensional approaches. The results included simulation of concentration, temperature, and reaction rate profiles via the 1-D and 2-D differential equations solution with FlexPDE software. The analysis was complemented with a scaled 3-D dynamic model implemented in COMSOL Multiphysics. Both FlexPDE and COMSOL Multiphysics relied on the finite element technique to solve the governing differential equations. The simulated concentration and temperature profiles from both FlexPDE and COMSOL models were compared to experimental data gathered from literature (specifically from a Fischer-Tropsch process to produce low-molecular-weight hydrocarbons in a configuration of cylindrical packed-bed reactors). Simulated concentration and temperature profiles from the 2-dimensional dynamic model and the COMSOL model were in good agreement with the trend observed in experimental data. Finally, the predicted reaction rate profiles from the COMSOL model and the 2-dimensional dynamic model followed the temperature trend, thus reflecting the temperature dependence of the reaction.

This paper focus on the modeling and time-domain analyzing method of piecewise-smooth fractional-order circuit systems. Firstly, an analytical framework is constructed based on a generalized piecewise-smooth two-port impedance network model, which takes the topological characteristics of Dickson type switched-capacitor converters (SCCs) as examples, and considers the fractional-order characteristics of class-2 X7R ceramic capacitors, which are widely used in SCCs. Secondly, the time-domain analyzing method for such a piecewise-smooth fractional-order circuit system is developed, numerical solutions are obtained, and the influence of the fractional-order capacitor on the power conversion efficiency of the system are revealed. Finally, in order to validate the analysis, both numerical simulation and experiments are provided in the work, the results of which confirm that, the proposed fractional-order analytical framework can capture the power loss mechanism of piecewise-smooth fractional-order circuit systems more effectively than the traditional integer-order based approach, thus benefiting the design of circuit systems.

Compared to conventional allothermal gasification of solid fuels (e.g. biomass, charcoal, lignite etc.), plasma-assisted gasification offers an efficient method to apply energy into the gasification process to increase the flexibility of operation conditions and to increase the reaction kinetics. In particular, non-thermal plasmas (NTP) are promising, in which thermal equilibrium is not reached and electrons have substantially higher mean energy than gas molecules. Thus it is generally assumed that in NTP the supplied energy is utilized more efficiently for generating free radicals initiating gasification reactions than thermal plasma processes. In order to investigate this hypothesis, we compared purely thermal to non-thermal plasma assisted gasification of biomass in steam in a drop tube reactor at atmospheric pressure. The NTP was provided by means of gliding arcs between two electrodes aligned in the inlet steam flow. Electric power of about 1 kW was supplied using a high voltage generator operating at frequencies between 70 and 150 kHz and voltage amplitudes up to 10 kV. A laser-assisted optical method (Raman spectroscopy) was applied for measuring the gas temperature both in the conventionally heated steam and flow-down of the visible plasma filaments of the gliding arcs. Reaction yields and rates were evaluated using these measured gas temperatures. The first experimental results have shown that the non-thermal plasma not only promotes the carbon conversion of the fuel particles, but also accelerates the reaction kinetics. The carbon conversion is increased by nearly 10% using wood powder as the fuel. With charcoal powder more than 3% are converted into syngas.

The paper presents a new type of hydraulic clutch operating with magnetorheological (MR) fluids and the results achieved from both theoretical analysis and experimental measurement. The hy-draulic clutch system of its own design with MR working fluid and a rotating magnetic field lo-cated is designed. The principle of this clutch is to use a rotating magnetic field created by an al-ternating current electromagnet to set the MR fluid in motion. In the tests of the hydraulic clutch with a rotating magnetic field, MR fluid produced on their own, consisting of solid iron particles of various diameters mixed with a silicone oil, are used as working fluids. The rheological properties of MR fluids are assessed on the basis of tests carried out with a Brookfield DV2T rheometer equipped with a magnetic device for generating the magnetic field of its own design. The char-acteristics of the hydraulic clutch with MR working fluid and the rotating magnetic field are tested on a specially built test stand. As a result of the research, it has been found that the torque transmitted by the clutch is the greater, the higher the rotational speed of the magnetic field and the lower the rotational speed of the beaker in which the working fluid is placed, and that the greatest torque occurs for the working fluid with the highest iron content. Based on the analysis of the structure and characteristics of the clutch in which the magnetic field is used, it has been shown that the design of the developed clutch is similar to that of an induction clutch, and its characteristics correspond to the characteristics of the eddy current clutch. Therefore, the proposed new clutch working with MR fluid and the rotating magnetic field can be applied to stationary power transmission system similarly to an eddy current clutch.

Due to their high specificity, monoclonal antibodies (mAbs) have garnered significant attention in recent decades, with advancements in production processes, such as high seeding density (HSD) strategies, contributing to improved titers. This study provides a thorough investigation of high seeding processes for mAb production in Chinese hamster ovary (CHO) cells. Using a metabolic network and flux balance analysis, we compared standard fed-batch (STD FB) with HSD cultivations, exploring supplementary lactate and cysteine, and a bolus medium enriched in amino acids. We observed high glycolytic fluxes and depletion of asparagine simultaneously with the lactate shift building kinetic models around this and other observations. An ensemble of kinetic models assessed the impact of a feeding medium enriched with additional asparagine, revealing a missing lactate shift without enhancing mAb productivity in the experiments. This research provides valuable model-based insights into cellular metabolism during HSD processes, laying the foundation for refined CHO cell-based mAb production. The proposed hypothesis on the regulations guides future experimental validation and bioprocess optimization for enhanced mAb production efficiency.

PHA synthases (PhaC) are grouped into four classes based on the kinetics and mechanisms of reaction. The grouping of PhaC enzymes into four classes is dependent on substrate specificity, according to the preference in forming short chain length (scl) or medium chain length (mcl) polymers: class I, class III, and class IV produce scl-PHAs depending on propionate, butyrate, valerate and hexanoate precursors, while class II phaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors. PHA synthases of class I, in particular PhaC_Cs from Chromobacterium USM2 and PhaC_Cn/RePhaC1 from Cupriavidus necator/R. eutropha, have been analysed and the crystal structures of the C-domains have been determined. PhaC_Cn/RePhaC1 was also studied by small angle X-ray scattering (SAXS) analysis. Models have been proposed for dimerization, catalysis mechanism, substrate recognition and affinity, product formation and product egress route. The assays based on amino acid substitution by mutagenesis have been useful to validate the hypothesis on the role of amino acids in catalysis and in accommodation of bulky substrates, for the synthesis of PHB co-polymers and medium chain length-PHA polymers with optimized chemical properties.

The kinetics and efficacy profiles of photoinitiated polymerization are theoretically presented. For the same dose, lower light intensity achieves a higher steady-state-efficacy (SSE) in type-I; in contrast, type-II has an equal SSE. Higher light intensity has a faster rising efficacy, due to faster depletion of photoinitiator (PS) concentration. However, type-II process is also affected by the available oxygen. Higher light intensity produces more efficient singlet oxygen, resulting a higher transient efficacy, in which all intensities reach the same SSE when oxygen is completely depleted. With external oxygen, type-II efficacy increases with time, otherwise, it is governed only by the light dose, i.e., same dose achieves same efficacy. Moreover, type-II has an efficacy follows Bunsen Roscoe law (BRL), whereas type-I follows non-BRL. The measured type-I efficacy and gelation profile are analyzed by our analytic formulas. Schematics of the photocrosslinking stage defined by the availability of oxygen is developed, where both type-I and –II coexist until the oxygen is depleted. The overall efficacy may be enhanced by resupply of PS or oxygen during the light exposure. The roles of light dose and PS concentration on the efficacy of photoinitiated polymerization should be are governed a new concept of a volume efficacy (Ve), defined by the product of the crosslink (or gelation) depth (CD) and local [efficacy].

Among the many Lysosomal Storage Disorders (LSDs) that would benefit from the establish-ment of novel cell models, either patient-derived or genetically engineered, is Mucopolysaccha-ridosis type II (MPS II). In fact, even though a specific therapeutic approach does exist for this disorder (Enzyme Replacement Therapy, ERT, with recombinant human IDS) and haematopoiet-ic stem cell transplantation (HSCT) may also hold promise whenever the disorder is diagnosed soon enough, both approaches have their own drawbacks. Here we present our results on the establishment and characterization of two MPS II pa-tient-derived stem cell line(s) from deciduous baby teeth. To the best of our knowledge, this is the first time a stem cell population is isolated from LSD patient samples obtained from the oral cavity. Taking into account our results on the molecular and biochemical characterization of those cells and our first insights on their subcellular MPS phenotype, we consider these cells will be a valuable tool for both pathophysiological assessments and drugs screenings. Ultimately, we believe patient-derived dental pulp stem cells (DPSC) may represent a feasible alternative to induced pluripotent stem cells (iPSC) in many labs with standard cell culture con-ditions and limited (human and economic) resources.

The aim of this work was to study the production of kefir-like beverage by fed-batch fermentation of red table grape juice at initial pHs of 3.99 (fermentation A) and 5.99 (fermentation B) with kefir grains during four repeated 24-h fed-batch subcultures. However, all kefir-like beverages (KLB) were characterized by low alcoholic grade (≤ 3.6%, v/v) and lactic and acetic acid concentrations. The beverages obtained from fermentation B had lower concentrations of sugars and higher microbial counts than the KLB obtained in fermentation A. In addition, the KLB from fermentation B were the most aromatic and had the highest contents in alcohols, esters, aldehydes and organic acids compared to the non-fermented juice and KLB from fermentation A. These results indicate the possibility of obtaining red table grapes KLB with their own distinctive aromatic characteristics and a high content in probiotic viable cells, contributing to the valorization of this fruit.

: Sulfate presence is of great importance for anaerobic digestion as its addition can control the anaerobic microbial community. In this study, the effect of sulfuric acid addition on the perfor-mance of an UASB reactor fed with hydrolyzed starch was investigated. TOC, Fe and SO4-2 re-moval as well as the methane production were monitored under various COD/ SO4-2 ratios, HRTs and OLRs. When HRT was 16 hours and OLR equal to 2 g COD/LUASB-d the methane production was 0.24 L CH4/LUASB-day. After the sulfuric acid addition, TOC removal reached 95%. At HRT, OLR, and COD/ SO4-2 ratio equal to 16 hours, 2 g COD/ LUASB-d and 3.72 respectively, the methane production was 0.267 L CH4/LUASB-d. When the OLR was increased to 5.94 g COD/LUASB-d and COD/SO4-2 ratio was equal to 12.5, the methane production was 3 times higher (0.84 L CH4/LUASB-d) with a methane content in the produced biogas greater than 70%. 16s rRNA gene analysis demonstrated that after the sulfate addition, SRB were enriched and methane was mainly produced by Methanosaeta sp. This study indicates that appropriate sulfate concentrations in the feed in combination with the presence of iron in the UASB reactor lead to enhanced me-thane yields.

For better understanding the corrosion and corrosion products behavior in the primary circuit of lead-bismuth eutectic (LBE) coolant reactor, the concentration distribution of soluble impurities and the transport of solid particles are investigated through finite-element method. An axisymmetric model of the primary circuit of LBE reactor was constructed to accelerate the calculation the thermal hydraulic filed of circuit. The saturation concentration of solute Fe, Cr and Ni in LBE coolant are identified through the equilibrium of their oxides and PbO. And the very different saturation concentrations of Fe/Cr/Ni in LBE will lead to significant element selective corrosion. The migration of solid oxides particles in the primary circuit is also investigated by the Euler-Lagrange tracing model. The simulation shows that driving force for the movement of particles >100 μm is buoyancy, which lets particles float on a free surface, while particles <10 μm tend to suspend in coolant. However, the behavior of particles also depends on the formation position, the particles formed above the core have the high possibility of re-entering in the core.

In this paper, two double Jordan-type inequalities are introduced based on the papers [1]-[5]. These inequalities generalize the inequalities obtained in [1]-[5]. As a result, some new upper and lower bounds of the sinc function are obtained. This extension of Jordan’s inequality is enabled by considering the corresponding inequalities through the concept of stratified families of functions elaborated in [6]. Based on this approach, some optimal approximations of the sinc function are derived by determining corresponding minimax approximants, also described in the paper [6].

Cyanobacteria are extensively studied and cultured because they can produce many value-added substances among which are pigments, mainly the phycobiliproteins phycocyanin (PC), phycoerythrin (PE), allophycocyanin (APC) and chlorophyll-a and carotenoids as well. As numerous cyanobacterial species await optimization for maximizing pigment production, we examined here two local marine species, Phormidium sp. and Cyanothece sp. batch cultured under 18-19.5 oC, at 40 ppt salinity with Walne’s nutrient medium, using white LED light of low (2000 lux) and high (8000 lux) intensity and additionally blue, green and red LED light. Significant differences were found among the intensities and colors of light used. Maximum growth was induced by high white light in both species (2.15 g dw/L in Phormidium and 1.47 g/L in Cyanothece). Next to them was green light (1.25 g/L) in Cyanothece and low white and green (1.26 – 1.33 g/L) in Phormidium. Green light maximized phycocyanin content in Phormidium (0.45 mg/mL), while phycoerythrin was maximized (0.17 mg/mL) by blue light and allophycocyanin by all colors (~0.80 mg/mL). All colors maximized phycocyanin in Cyanothece (~0.32 mg/mL) while phycoerythrin and allophycocyanin were maximized under green light (~0.138 and 0.38 mg/mL respectively). In Phormidium maximization of chlorophyll-a (9.3 μg/mL) was induced by green light while total carotenoids and b-carotene (3.05 and 0.89 μg/mL respectively) by high white light. In Cyanothece both white light intensities along with green light maximized chlorophyll-a content (~9 μg/mL) while high white light and green maximized total carotenoids (2.6-3.0 μg/mL).

This study aimed to elucidate the similarities and differences between amyloid-forming corpora amylacea (CA) in the prostate and lung, examine the nature of CAs in cystic tumors of the atrioventricular node (CTAVN), and clarify the distinctions between amyloid-forming CA and spheroid-type amyloid deposition. We conducted proteomics analyses using liquid chromatography–tandem mass spectrometry with laser microdissection and immunohistochemistry to validate the characteristics of CAs in the lung and prostate. Our findings revealed that CAs in these organs primarily consist of common proteins (β2-microglobulin and lysozyme) and locally produced proteins. Moreover, we observed a discrepancy between the histopathological and proteomic analysis results in CTAVN-associated CAs. In addition, while the histopathological appearance of amyloid-forming CAs and spheroid-type amyloid deposits were nearly identical, the latter deposition lacked β2-microglobulin and lysozyme, and exhibited evident destruction of surrounding tissue. A literature review further supported these findings. These results suggest that amyloid-forming CAs in the lung and prostate are formed through a shared mechanism, serving as waste containers (wasteosomes) and/or storage for excess proteins (functional amyloids). In contrast, we hypothesize that while amyloid-forming CA and spheroid-type amyloid deposits are formed, in part, through common mechanisms, the latter are pathological.

Traditional aquaculture systems appear to be confronted with the high total ammonia nitrogen (TAN) produced during production, which can be harmful to aquatic life. As the demand for global fish production continues to increase, farmers should adopt Recirculating Aquaculture Systems (RAS) to improve production. The biofilter plays a vital role in ammonia removal. However, the drawback of the biofilter operation is needed an automatic and controlled system with a water quality monitoring system to guarantee optimal performance. RAS utilize a monitoring and control system to maintain the water quality and control the biofilm growth in the biofilter to reduce the ammonia. Therefore, this study focuses on developing a water quality monitoring and control system to achieve optimum biofilm performance. From 35 days of the experiment, the water quality was controlled at a level save for the aquatic environment, such as dissolved oxygen, pH, temperature, and TDS. Thus, the highest TAN removal efficiency is 50% when biofilm thickness reaches 119.88 µm.

Background Homologous recombination deficiency (HRD) evolved into a major diagnostic marker in high grade ovarian cancer (HGOC) predicting the response to poly (Adenosine diphosphate-ribose) polymerase inhibitors (PARPi) and also platinum-based therapy. In addition to HRD, the type of peritoneal tumor spread influences treatment response and patient survival; miliary type tumor spread has poorer predicted outcome than non-miliary type tumor spread. Methods Known methods for HRD assessment were adapted for our technical requirements and the Predictive-value Integrated Genomic Instability Score (PIGIS) for HRD assessment evolved as an outcome. PIGIS was validated in HGOC samples from 122 patients. We used PIGIS to analyze whether the type of tumor spread correlated with HRD status and whether this had an impact on survival. Results We demonstrated that PIGIS can discriminate HRD positive from HRD negative samples. Tumors with miliary tumor spread are HRD negative and have a very bad prognosis with a progression free survival (PFS) of 15.6 months and an overall survival (OS) of 3.9 years. However, HRD negative non-miliary spreading tumors in our cohort had a much better prognosis (PFS 35.4 months, OS 8.9 years), similar to HRD positive tumors (PFS 34.7 months, OS 8.9 years). Conclusions Our results indicate that in a predominantly PARPi naïve cohort the type of tumor spread might be a better predictor of response to platinum sensitivity than HRD. It remains to be determined, whether this also applies for sensitivity to PARPi.

On the H-type group G⊂Rm×Rn, we study the existence of solutions for the Brezis-Nirenberg problem

The licensing of new reactors implies the use of verified and validated neutronics codes. Numerical validation can rely on sensitivity and uncertainty studies but they require repeated execution of time-consuming neutron flux and depletion calculations. The computational costs can be shortened by using perturbation theories. However, Depletion Perturbation Theory is restricted to single integral values such as a nuclide density. Relying on reduced basis approaches, which reconstruct the whole nuclide densities at once, is one way to get around this restriction. Furthermore, the adjoint-based reduced-order model uses the direct and adjoint equations for the projection. For diffusion or transport calculations, Exact-to-Precision Generalized Perturbation Theory has been developed. Still, no models for depletion calculations are readily available. Therefore, this paper describes a novel adjoint-based reduced order model for the Bateman equation. It uses a range-finding algorithm to create the basis and the Depletion Perturbation Theory for the reconstruction of the nuclide densities at first order. Our paper shows that for several perturbed cases, the depletion reduced-order model successfully reconstructs the nuclide densities. As a result, this serves as a proof of concept for our adjoint-based reduced order model which can perform sensitivity and uncertainty burn-up analysis in a shorter time.

The UK government and many international experts have pointed out that nuclear energy has an important role to play in the transition towards a decarbonized energy system since it is the only freely manageable very low-carbon energy technology with 24/7 availability to complement renewables. Besides current investments in light water reactor technologies, we need innovation for improved fuel usage and reduced waste creation, like offered by iMAGINE, for the required broad success of nuclear technologies. To allow quick progress into innovative technologies like iMAGINE and their regulation, a timely investment into urgently needed experimental infrastructure and expertise development will be required to assure the availability of capacities and capabilities. The initial steps to start the development of such a new reactor physics experimental facility to investigate molten salt fast reactor technology are discussed and a stepwise approach for the development of the experimental facility is described. The down selection to the choice for a diverse control and shutdown system is described through manipulating the reflector (control) and splitting the core (shutdown). The developed innovative core design of having the two core parts in two different rooms opens completely new opportunities and will allow to manifest the request for separated operational and experimental crews, as nowadays requested by regulators into the built environment. The proposed physical separation of safety relevant operational systems from the experimental room should on the one hand help to ease the access to the facility for visiting experimental specialists. On the other hand, the location of all safety relevant systems in a now separated access-controlled area for the operational team will limit the risk of misuse through third party access. The planned experimental programme described with the major steps: core criticality experiments, followed by experiments to determine the neutron flux, neutron spectrum, and power distribution as well as experiments to understand the effect of changes in reactivity and flux as a function of salt density, temperature and composition change.

The morphology of virgin reactor powder (RP) of high-density polyethylene HDPE with MW = 160,000 g/mol was investigated with the help of DSC, SEM, SAXS and WAXS methods. The morphological SEM analysis showed, that the main morphological units of RP are macro- and micro-shish kebab structures with significantly different geometric dimensions, as well as individual lamellae of folded chain crystals. Quantitative analysis of an asymmetric SAXS reflection made it possible to reveal the presence of several periodic morphoses in the RP with long periods from 20 nm to 60 nm and to correlate them with the observed powder morphology. According to the DSC crystallinity data, the thickness of the lamellae in each long period was estimated. Their surface energy was calculated in the framework of the Gibbs-Thompson theory. The presence of regular and irregular folds on the surface of the different shish-kebab lamellae is discussed. The percentage of identified morphoses in the RP was calculated. It has been suggested that the specific structure of HDPE RP is due to the peculiarity of polymer crystallization during suspension synthesis in a quasi-stationary regime, in which local overheating and inhomogeneous distribution of shear stresses in a chemical reactor are possible.

Molten salt reactors have gained substantial interest in the last years due to their flexibility and their potential for simplified closed fuel cycle operation for massive net-zero energy production. However, a zero-power reactor experiment will be an essential first step into the process of delivering this technology. The topic of the control and shut down for a zero power reactor is for the first time introduced through a literature review and the reduction of the control approaches to a limited number of basic functions with different variations. In the following, the requirements for the control and shutdown system for a reactor experiment are formulated, and based on these assessments, an approach for the shutdown – splitting the lower part of the core with reflector, and an approach for the control – a vertically movable radial reflector are proposed. Both systems will be usable for a zero-power system with a liquid as well as with as a solid core and even more importantly, both systems somehow work on the integral system level without disturbing the central part of the core which will be the essential area for the experimental measurements. Both approaches have been investigated as a singular system as well as their interactions with one another and the sensitivity of the control system. The study has demonstrated that both proposed systems are able to deliver the required characteristics with sufficient shutdown margin and a sufficiently wide control span. The interaction of the system has been shown to be manageable and the sensitivity is on a very good level. The multi-group Monte-Carlo approach has been cross evaluated by a continuous energy test leading to good results, but they also demonstrate that there is room for improvement.

Computationally modelling a nuclear reactor startup core for a benchmark against the existing models is highly desirable for an independent assessment informing nuclear engineers and energy policymakers. This work presents a startup core model of the UK’s first Evolutionary Pressurised Water Reactor (EPR) based on Monte Carlo simulations of particle collisions using Serpent 2, a continuous-energy Monte Carlo reactor physics burnup code. Coupling between neutronics and thermal-hydraulic conditions with the fuel depletion is incorporated into the multi-dimensional branches, obtaining the thermal flux and fission rate (power) distributions radially and axially from the three dimensional (3D) single assembly level to a 3D full core. Shannon entropy is employed to characterise the convergence of the fission source distribution, with 3 billion neutron histories tracked by parallel computing. Source biasing is applied for the variance reduction. Benchmarking the proposed Monte Carlo 3D full-core model against the traditional deterministic transport computation suite used by the UK Office for Nuclear Regulation (ONR), a reasonably good agreement within statistics is demonstrated for the safety-related reactivity coefficients, which creates trust in the EPR safety report.

An effective method to inspect inaccessible nuclear power facility by interface wave which propagate along the shrink fit boundary of reactor head is proposed in this study. Reactor head is relatively thick to inspect from the outside of reactor by conventional ultrasonic testing. The proposed interface wave can propagate a long distance from the fixed transducer position. The inside of nuclear reactor is limited to access due to the high radiation, so transducers are located at outside of nuclear facility and interface wave propagates into the nuclear reactor for defect detection. The numerical simulation and experiments were carried out to validate the effectiveness of the proposed interface wave inspection method. Various defect cases simulating field failures are also presented with satisfactory detectability by the proposed technique with the features for defect classification.

Orthohepadnavirus causes chronic hepatitis in a broad range of mammals, including primates, cats, woodchucks, and bats. Hepatitis B virus (HBV) X protein inhibits type-I interferon (IFN) signaling, promoting HBV escape from the innate immune system to establish persistent infection. However, whether X proteins of Orthohepadnavirus viruses in the other species have similar inhibitory activity remains unknown. Here, we investigated the anti-IFN activity of 17 Orthohepadnavirus X proteins derived from various hosts. We observed conserved activity of Orthohepadnavirus X proteins in inhibiting TIR-domain-containing adaptor protein inducing IFN-β (TRIF)-mediated IFN-β signaling pathway through TRIF degradation. X proteins from domestic cat hepadnavirus (DCH), a novel member of Orthohepadnavirus, inhibited mitochondrial antiviral signaling protein (MAVS)-mediated IFNβ signaling pathway comparable with HBV X. These results indicate that inhibition of IFN signaling is conserved in Orthohepadnavirus X proteins.

Hepatocellular carcinoma (HCC) is a heterogeneous malignancy with complex carcinogenesis. Although there has been significant progress in the treatment of HCC over the past decades, drug resistance to chemotherapy remains a major obstacle in its successful management. In this study we were able to reduce chemoresistance in cisplatin-resistant HepG2 cells by either silencing the expression of transglutaminase type 2 (TG2) using siRNA or by pre-treatment of cells with the TG2 enzyme inhibitor cystamine. Further analysis revealed that, whereas the full-length TG2 isoform (TG2-L) was almost completely cytoplasmic in its distribution, the majority of the short TG2 isoform (TG2-S) was membrane-associated in both parental and chemoresistant HepG2 cells. Following induction of cisplatin toxicity in non-chemoresistant parental cells, TG2-S together with cisplatin quickly relocated to the cytosolic fraction. Conversely, no cytosolic relocalisation of TG2-S or nuclear accumulation cisplatin was observed following identical treatment of chemoresistant cells, where TG2-S remained predominantly membrane-associated. This suggests that deficient subcellular relocalisation of TG2-S from membranous structures into the cytoplasm may limit the apoptic response to cisplatin toxicity in chemoresistant cells. Structural analysis of TG2 revealed the presence of binding motifs for interaction of TG-S with the membrane scaffold protein LC3/LC3 homologue that could contribute to a novel mechanism of chemotherapeutic resistance in HepG2 cells.

Nanoporous materials, such as Metal-Organic Frameworks (MOFs), are renowned for their high selectivity as gas adsorbents due to their specific surface area, nanoporosity, and active surface chemistry. A significant challenge for their widespread application is their behavior and reduced gas uptake in wet conditions, attributed to competitive adsorption between gas and water. The current understanding of gas adsorption in wet materials is constrained by studies that utilize only small amounts of powdered porous materials (in the milligram range) within very small reactors (1-5 mL). This leaves a significant gap in knowledge about gas adsorption behaviors in larger reactors and when MOF sample sizes are increased (to the gram scale) for scaling up the process. Additionally, there is a notable dearth of experimental research in situations where MOFs are heavily saturated with water. To address these gaps, this study measures CH4 adsorption in MOFs under conditions of high moisture content, using larger samples of MOFs (in grams) and a large volume reactor. We selected commercially available MOFs, including HKUST-1, ZIF-8, MOF-303, and activated carbon. A high-pressure isotherms (at T = 274.15 K) using volumetric approach were measured to qualitatively compare the moles of gas adsorbed under both dry and wet conditions across different MOFs and weights. Experimental results show that the presence of water led to a decrease in total CH4 mmol adsorption in MOFs, with a more significant decrease observed in hydrophilic MOFs compared to hydrophobic ones. For hydrophilic MOFs, presence of crossover isotherms at crossover pressure provided evidence of water's conversion to hydrate and its positive role in enhancing total gas uptake, which varied among different hydrophilic MOFs. Oversaturated MOFs showed a larger deviation between dry and wet cases at higher pressures. The high-pressure isotherm data confirm the positive role of hydrophilic MOFs in the conversion of water to hydrate in oversaturated MOFs when MOFs sample size is large.

Molten salt reactors have gained substantial interest in the last years due to their flexibility and their potential for simplified closed fuel cycle operation for massive net-zero energy production. The importance of a zero-power reactor for the process of developing a new, innovative rector concept like molten salt fast reactor is described here. It is based on historical developments as well as the current demand for experimental results and key factors that are relevant to the success of the next step in the development process of all innovative reactor types. In the systematic modelling & simulation of a zero-power molten salt reactor, the radius and the feedback effects are studied for a eutectic based system, while a heavy metal rich chloride-based system are studied depending on the uranium enrichment accompanied with the effects on neutron flux spectrum and spatial distribution. These results are used to support the relevant decision for the narrowing down of the configurations supported by considerations on cost and proliferation for the follow up 3D analysis to provide for the first time a systematic modelling & simulation approach for a new reactor physics experiment. The expected core volumes for these configurations have been studied using multi-group and continuous energy Monte-Carlo simulations identifying the 35% enriched systems as the most attractive and finally leading to the choice of the heavy metal rich compositions 35% enrichment as the reference system for future studies of the next steps in the zero power reactor investigation. The inter-comparison of the different applied codes and approaches available in the SCALE package has delivered a very good agreement between the results creating trust into the developed and used models and methods.

The study experimentally investigated a novel approach for producing hydrogen from methane cracking in dielectric barrier discharge catalytic plasma reactor using a nanocatalyst. Plasma-catalytic methane (CH4) cracking was undertaken in a dielectric barrier discharge (DBD) catalytic plasma reactor using Ni/MgAl2O4. The Ni/MgAl2O4 was synthesised through co-precipitation followed customised hydrothermal method. The physicochemical properties of the catalyst were examined using X-ray diffraction (XRD), scanning electron microscopy - energy dispersive X-ray spectrometry (SEM-EDX) and thermogravimetric analysis (TGA). The Ni/MgAl2O4 shows a porous structure spinel MgAl2O4 and thermal stability. In the catalytic-plasma methane cracking, the Ni/MgAl2O4 shows 80% of the maximum conversion of CH4 with H2 selectivity 75%. Furthermore, the stability of the catalyst was encouraging 16 hours with CH4 conversion above 75%, and the selectivity of H2 was above 70%. This is attributed to the synergistic effect of the catalyst and plasma. The plasma-catalytic CH4 cracking is a promising technology for the simultaneous H2 and carbon nanotubes (CNTs) production for energy storage applications.

A four stage semi-pilot scale RFR reactor with ceramic disks as support for TiO₂ modified with silver particles was developed for the removal of organic pollutants. The design presented in this article is an adaptation of the rotating biological reactors (RBR) and its coupling with the modified catalyst provides additional advantages to designs where a catalyst in suspension is used. The optimal parameter of rotation was 54 rpm and the submerged surface of the disks offer a total contact area of 387 M². The modified solid showed a decrease in the value of its bandgap compared to commercial titanium. The system has a semi-automatic operation with a maximum reaction time of 50 h. Photo-activity tests show high conversion rates at low concentrations. The results conform to the Langmuir heterogeneous catalysis model.

This paper aims to study the performance of solar collectors with various sizes under different weather conditions of different Japanese cities, i.e. Kofu city, Nagoya city and Yamagata city. The heat generated from the solar collector was used to conduct a biogas dry reforming reactor in order to produce H2 to feed a solid oxide fuel cell (SOFC). This study has revealed that the output temperature of solar collector, Tfb in April and July was higher than that in January and October irrespective of city. The optimum length of absorber (dx) of the collector was 4 m irrespective of city. It was clarified that Tfb in Yamagata city in January and October, i.e. winter and autumn is lower than that in Kofu city and Nagoya city especially, which is influenced by the tendency of a solar intensity (I) strongly, not velocity of surrounding air (ua). On the other hand, Tfb is almost the same in April and July, i.e. spring and summer, irrespective of city. The amount of produced H2 from the biogas dry reforming reactor and the power generated by SOFC using H2 in spring and summer were higher compared to the other seasons irrespective of city. This study has revealed that the highest available households number per month was 4.7 according to the investigation in this study.

The combination of artificial intelligence algorithms and numerical methods has recently become popular in the prediction of macroscopic and microscopic hydrodynamics parameters of bubble column reactors. The multi inputs and outputs machine learning can cover small phase interactions or large fluid behavior in industrial domains. This numerical combination can develop the smart multiphase bubble column reactor with the ability of low-cost computational time. It can also decrease case studies for the optimization process when big data is appropriately used during learning. There are still many model parameters that need to be optimized for a very accurate artificial algorithm, including data processing and initialization, the combination of inputs and outputs, number of inputs and model tuning parameters. For this study, we aim to train four inputs big data during learning process by an adaptive neuro-fuzzy inference system or adaptive-network-based fuzzy inference system (ANFIS) method, and we consider the superficial gas velocity as one of the input variables, while for the first time, one of the computational fluid dynamics (CFD) outputs named gas velocity is used as an output of the artificial algorithm. The results show that the increasing number of input variables improves the intelligence of the ANFIS method up to , and the number of rules during learning process has a significant effect on the accuracy of this type of modeling. The results also show that propper selection of model parameters results in more accuracy in prediction of the flow characteristics in the column structure.

(1) Background Diabetic Retinopathy (DR) is the primary cause of poor vision in young adults. Screening for DR is important but many patients do not attend; (2) Methods: We validated an algorithm for predicting DR and its progression by conducting a retrospective study of 11 children in a population of 120,389 T2DM (3) Results: By applying the algorithm to the population showed an AUC of 0.93 (95% CI, 0.92–0.94) for any-DR and 0.90 (95% CI, 0.89–0.91) for referable-DR(4) Conclusions: The algorithm is useful for predicting patients who could develop referable forms of DR and also for any-DR. This would allow a personalized screening plan to be drawn up for each patient.

D-type cyclins encode G1/S cell cycle checkpoint proteins, that play a crucial role in defining cell cycle exit and progression. Precise control of cell cycle exit is vital during embryonic development, with defects in the pathways regulating intracellular D-type cyclins resulting in abnormal initiation of stem cell differentiation in a variety of different organ systems. Furthermore, stabilisation of D-type cyclins is observed in a wide range of disorders characterised by cellular over-proliferation, including cancers and overgrowth disorders. In this review, we will summarise and compare the roles played by each D-type cyclin during development and provide examples of how their intracellular dysregulation can be an underlying cause of disease.

Lacunary polyoxometalates (POMs) are negatively charged metal−oxo clusters, formally obtained from plenary topologies via fragment removal. Owing to the fragment removal, the lacunary POMs archetypes are rich in nucleophilic terminal oxo ligands, making them suitable for post-synthetic coordination with various heterometals. Trilacunary heteropolytungstates (het-ero-POTs) based on bowl-shaped {W9O30} framework incorporating a central lone-pair containing {XO3} hetergroup (X = AsIII, SbIII, and BiIII) function as all-inorganic scaffolds that in the presence of d-block metal cations typically construct sandwich-like dimers of Hervé and/or Krebs. Herein we review the preparative approaches, compositional and magnetic versatility of the constructing Hervé and Krebs type dimers and discuss prospective use as POMtronics.

This article introduces an innovative design method for public lighting systems that surpasses the limitations of conventional approaches, which rely on predefined lamp characteristics and spatial arrangement. By employing a multi-criteria decision algorithm, our study proposes an optimal design methodology considering several key aspects, including the distance between lamps, their type, power, and light distribution. The goal is to achieve optimal illumination that enhances visibility on public roads for drivers and pedestrians while simultaneously minimizing glare, installation costs and maximizing energy efficiency. The proposed methodology is implemented through an algorithm developed in Matlab, with results validated through simulations in Dialux. This information is used to construct a decision matrix, assessed using the CRITIC method across 180 different scenarios within a specific case study. The findings demonstrate the effectiveness of multi-criteria decision-making as a tool for significantly improving the planning and design of lighting in public illumination systems, allowing for selecting the optimal combination of parameters that ensure the best lighting conditions.

Background Apolipoprotein E (APOE) gene polymorphism has been implicated in the pathogenesis of various metabolic disorders, including type 2 diabetes mellitus (T2DM). Type 2 diabetes mellitus (T2DM) is a major public health concern worldwide, including in Pakistan. Cardiovascular problems linked with T2DM have a significant impact on people and society. The study's goal is to investigate the relationship between Apolipoprotein E (ApoE) genotypes, lipid profiles, and cardiovascular complications such as ischemic heart disease (IHD) and stroke. Methods: This study was carried out on 260 subjects, divided into control D1: Diabetics without cardiovascular issues. D2: Diabetics with heart disease. D3: Diabetics with stroke. D4: Diabetics with both heart disease and stroke. Anthropometric parameters (age, BMI) and risk factors (smoking, diabetes duration, hypertension) were assessed in all groups. Serum levels of TC, TG, LDL, HDL, VLDL, creatinine, BSF, and HbA1c were measured. Apolipoprotein E gene polymorphism was determined using PCR-RFLP. Results: Hypertension, BMI, and dyslipidemia, defined as elevated levels of total cholesterol, triglycerides, LDL, HDL, and VLDL, as well as uncontrolled hyperglycemia (elevated fasting blood sugar and glycated hemoglobin) in T2DM, were linked to vascular complications such as IHD and stroke. Hypertension was prevalent in 75.4% of the population, with greater rates among the elderly. Stage 2 hypertension was more prevalent in all age groups. Apolipoprotein E gene polymorphism was discovered, with genotypes 3/3, 4/4, 2/3, and 3/4 being the most prevalent. Genotype 3/4 was more common in diabetics who had IHD/stroke or both problems, whereas genotype 2/3 was linked to DM and stroke. Conclusion: It is concluded that BMI, hypertension, hyperglycemia, atherosclerosis, and dyslipidemia are linked with cardiovascular complications of type 2 diabetes. Apolipoprotein E gene polymorphism is associated with cardiovascular disease in diabetic patients by affecting the lipid profile.

The economics and safety of reactors can be affected by the diffusion of fission products into graphite. Xenon (Xe) fission products diffusing into the graphite is the most critical neutron absorber and poison that can slow down or stop the chain reaction. The transport parameters for inhibiting the xenon diffusion in graphite are therefore the utmost scientific problem. Self-sintered nanopore-isotropic (~ 40nm) graphite (SSNG) derived from green pitch coke can decrease Xe diffusion into the graphite. In this study, a method for measurement of diffusion coefficients of fission products diffusion in graphite by Rutherford backscattering spectrometry (RBS) was reported. The SSNG substrates were implanted with Xe at a dose of 4.8×1015 ions/cm2 and energy of 7 MeV. The RT-implanted samples were annealed in a vacuum at 650°C for 9 h. The implanted and annealed samples were characterized by RBS. The diffusion coefficient D(Xe,650°C) is 6.49×10-20 m2/s. The results indicate the excellent ability to inhibit Xe diffusion of the SSNG and present significance in designing and evaluating the safety of nuclear reactors.