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Preprint ARTICLE | doi:10.20944/preprints202007.0740.v1

Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow

Zachary P Morehouse, Caleb M Proctor, Gabriella L Ryan, Rodney J Nash

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Virus Detection; Viral Diagnostics; Diagnostics; SARS-CoV-2; Coronavirus; PCR

Online: 31 July 2020 (09:31:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1468.v1

Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR

Claudio A. Henriquez, Natalia Riquelme, Alejandra Larach, Ximena Besoain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Virus-viroid detection; Mixed infections; Comparison of diagnostic methods; CTV; HSVd; Bioamplification

Online: 19 January 2024 (07:52:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0113.v1

Integrated 0.35 µm CMOS Control Circuits for High Performance Voltage-Mode DC-DC Boost Converter

Chan-Soo Lee, Munkhsuld Gendensuren, Bierng-Chearl Ahn, Seong-Gon Choi

Subject: Engineering, Electrical And Electronic Engineering Keywords: CMOS; DC-DC converter; control circuit; integration; voltage –mode; boost converter

Online: 7 November 2022 (08:48:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0688.v1

Direct Quantitation of SARS-CoV-2 Using Droplet Digital PCR in Suspected Samples With Very Low Viral Load

Michela Deiana, Chiara Piubelli, Antonio Mori, Gian Paolo Chiecchi, Giulia La Marca, Martina Leonardi, Davide Treggiari, Anna Beltrame, Lucia Moro, Zeno Bisoffi, Elena Pomari

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: droplet digital PCR; real time RT-PCR; SARS-CoV-2; false negative; viral load; diagnosis

Online: 26 April 2021 (20:09:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0387.v1

Acquisition of Humoral Immune Responses in Convalescent Japanese People with SARS-CoV-2 (COVID-19) Infection in 2021

Koshiro Monzen, Takanori Watanabe, Toshihiro Okabe, Hisakuni Sekino, Hironori Nakagami, Ryuichi Morishita

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2; Convalescent serum; Neutralizing antibody

Online: 4 August 2023 (12:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1328.v1

Finding a Direct method for a Dynamic process: the DD (Direct and Dynamic) Cell-Tox method

Eneko Madorran, Lidija Kocbek Šaherl, Mateja Rakuša, Mihael Munda

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: cell viability; in vitro toxicology; dynamic direct cell death assay

Online: 22 April 2024 (08:36:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0030.v1

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi, Hussein Alburkat, Taisir Abdulelah Kadhim, Ammar M S Al-Helli, Mustafa Hamza Ghazi, Yasir Abdulameer N. Aldabbagh, Reem Numaan Talib, Sama Hussein Ali, Noor Hameed Imran, Ruaa Almohsen, Turki Muftin Saad, Pallavi Mandave

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: PCR; Salmonella Typhi; Widal Test; BHI

Online: 1 November 2022 (12:43:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0262.v1

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Ju Huang, Ligong Zhai, Junyin Wang, Xiaotian Sun, Baoshi Wang, Zhaohui Wei

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Pseudomonas aeruginosa; Pseudomonas fragi; simultaneous detection; droplet digital PCR

Online: 3 April 2024 (16:17:11 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202405.0366.v1

Direct Cold Sintering of α-Al₂O₃ Ceramics in a Pure Water Medium

Anastasia A. Kholodkova, Maxim V. Kornyushin, Arseniy N. Khrustalev, Levko A. Arbanas, Andrey V. Smirnov, Yurii D. Ivakin

Subject: Chemistry And Materials Science, Ceramics And Composites Keywords: gibbsite; boehmite; alumina; cold sintering process; porous ceramics

Online: 7 May 2024 (10:22:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0243.v1

Useful Predictor for Exacerbation of Esophagogastric Varices after Hepatitis C Virus Eradication by Direct-Acting Antivirals

Yuko Nagaoki, Kenji Yamaoka, Yasutoshi Fujii, Shinsuke Uchikawa, Hatsue Fujino, Atsushi Ono, Eisuke Murakami, Tomokazu Kawaoka, Daiki Miki, Hiroshi Aikata, C. Nelson Hayes, Masataka Tsuge, Shiro Oka

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: Direct-acting antiviral; sustained virological response; esophagogastric varices; liver stiffness measurement; total bile acid; left gastric vein

Online: 5 June 2024 (10:53:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0757.v1

Direct and Indirect Effects of Mountain Heights on Heavy Rainfall in the Hokitika Region of New Zealand

Yang Yang, Ian Boutle, Stuart Moore, Trevor Keith Carey-Smith, John Crouch

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: Heavy rainfall; orographic lifting; mountain blocking; cold front; sub-grid mountain height; dual-polarisation radar; Southern Alps

Online: 13 March 2024 (07:28:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0032.v1

Transcranial Direct Current Stimulation: Effects on Motor and Non-Motor Symptoms of Parkinson's Disease

Fayda Protásio, Carlos Tomaz, Joaquim Brasil-Neto, Pedro Brandão, Nasser Allam, Maria Clotilde Henriques Tavares

Subject: Biology And Life Sciences, Behavioral Sciences Keywords: Parkinson’ s disease; neuromodulation; transcranial direct current stimulation – tDCS; movement disorders; mood; sleep; cognition

Online: 1 April 2024 (09:56:57 CEST)

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Abstract Introduction: In the last thirty years, brain neuromodulation techniques have been used as an alternative to pharmacological treatment of neurological disorders. Parkinson's disease (PD) is a neurodegenerative disorder leading to bradykinesia, rest tremor, postural changes, and non-motor symptoms such as depression, anxiety, sleep disorders, pain, and cognitive decline that compromises executive functions (EFs), responsible for the orderly execution of behaviors and tasks of daily life and intentional and directed actions. To this date, a few studies with transcranial direct current stimulation (tDCS) have shown beneficial effects in PD patients concerning specific motor and non-motor symptoms, targeting the motor cortex and/or prefrontal regions. Objective: The main objective of this study was to evaluate the effects of left prefrontal tDCS across a broad spectrum of motor and non-motor symptoms of PD using established validated scales. Method: Single-blind randomized clinical trial with 18 volunteers with PD, aged between 45 and 80 years (66.1 ± 9.65), who met inclusion and exclusion criteria. Participants were submitted to assessments of motor and non-motor functions employing psychometric scales and tests to evaluate EFs and were randomly divided into two groups: control (sham stimulation) and experimental (active stimulation). All participants were involved in three separate tDCS sessions. The anode was positioned over the left dorsolateral prefrontal cortex and the cathode over the right supraorbital region, with a direct current intensity of 2mA, lasting 20 minutes. At the end of the three sessions, all participants were reassessed. Results: Significant effects of tDCS on non-motor functions were observed for cognition (verbal fluency of actions, clock copy test, appointment by visual confrontation, and verbal memory with immediate free recall) and subjective assessment of sleep quality (overall restlessness and discomfort in the arms and legs at night, leg and arm cramps at night and distressing dreams). There was also an improvement in the rate of errors and successes for congruent and incongruent stimuli of the Stroop Test. The beneficial effects on motor function were decreased rigidity, improved gait, and greater agility in the finger-tapping test. Conclusion: Three tDCS sessions showed positive results for participants with PD, producing significant improvements in various motor and non-motor functions, including sleep quality, cognition, and EFs. Additionally, the present results indicate that tDCS neuromodulation of the left dorsolateral prefrontal cortex region is feasible, safe, and provides significant objective benefits for PD patients.

Preprint ARTICLE | doi:10.20944/preprints202104.0299.v1

Optimizing Droplet Digital PCR for Environmental Samples

Vasilis Kokkoris, Eric Vukicevich, Andrew Richards, Corrina Thomsen, Miranda Hart

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ddPCR, droplet digital PCR, environmental samples, environmental DNA, soil microbiology

Online: 12 April 2021 (12:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0203.v1

Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken

Subject: Biology And Life Sciences, Insect Science Keywords: Tungiasis; Tunga penetrans; molecular entomology; DNA isolation; Phusion® polymerase; FIREpol® Taq polymerase; low-cost PCR,

Online: 10 November 2022 (11:19:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.0207.v1

Ordered versus Non-Ordered Mesoporous CeO₂-Based Systems for the Direct Synthesis of Dimethyl Carbonate from CO₂

Nicoletta Rusta, Fausto Secci, Valentina Mameli, Carla Cannas

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: CO₂ utilization; dimethyl carbonate; ceria; mesoporous; nanocomposites; catalysis

Online: 6 August 2024 (03:39:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0776.v1

Neutral Red Film Augments Direct and Mediated Extracellular Electron Transfer Performed by Clostridium pasteurianum DSM 525

Ana Clara Bonizol Zani, João Carlos de Souza, Adalgisa Rodrigues de Andrade, Valeria Reginatto

Subject: Chemistry And Materials Science, Electrochemistry Keywords: Clostridium pasteurianum DSM 525; glycerol; mediated electron transfer (MET); neutral red (NR); methyl viologen (MV)

Online: 10 September 2024 (15:18:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1182.v1

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Laura Sancha Domínguez, Ana Cotos Suárez, María Sánchez Ledesma, Juan Luis Muñoz Bellido

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: dPCR; digital PCR; infection; bacteria; virus; parasites

Online: 20 March 2024 (08:24:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0431.v1

Blind Cyclostationary Based Carrier Number and Spacing Estimation for Carrier-Aggregated Direct Sequence Spread Spectrum Cellular Signals

Ali Görçin

Subject: Engineering, Electrical And Electronic Engineering Keywords: cyclostationary feature detection; blind parameter estimation; wireless signals; direct sequence spread spectrum; carrier aggregation

Online: 5 September 2024 (08:15:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1030.v1

Impact of Treatment with Direct Antivirals on Coagulation Parameters in Patients with HCV-related Liver Cirrhosis and Sustained Virological Response

Laura Huiban, Carol Stanciu, Cristina Maria Muzica, Irina Girleanu, Raluca Ioana Avram, Ioana Roxana Damian, Robert Nastasa, Ermina Stratina, Sebastian Zenovia, Horia Minea, Remus Stafie, Adrian Rotaru, Ana-Maria Singeap, Stefan Chiriac, Ioana Miruna Balmus, Anca Trifan

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: coagulation parameters; procoagulant factors; anticoagulant factors; chronic hepatitis C infection; direct-acting antivirals therapy; sustained virological response; liver cirrhosis

Online: 15 August 2024 (02:46:36 CEST)

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Background and Aims: Sustained virologic response (SVR) lead to the decrease of portal hypertension, the regression of fibrosis, the improvement of the hepatic synthesis of procoagulant and anticoagulant factors. We aimed to assess the influence of SVR on coagulation parameters in HCV cirrhotic patients treated with DAAs. Methods: We performed a prospective study in the Institute of Gastroenterology and Hepatology Iasi, Romania, between January 2022 and February 2024. We included patients diagnosed with compensated and decompensated HCV-related liver cirrhosis, treated with direct antivirals (PrOD ± RBV or SOF/LED ± RBV) for 12/24 weeks. Blood samples for biochemical, immunological and coagulation tests were collected at baseline, EOT and SVR12/24. Results: We analyzed a group of 52 patients with HCV-related liver cirrhosis, predominantly female (68.0%), the degree of severity of cirrhosis placed the patients mainly in Child-Pugh classes B (40%) and C (36%). All patients achieved SVR. The MELD score decrease at EOT (13.48 ± 4.273; p =0.001), and SVR (9.88 ± 2.774; p = 0.000), compared to baseline (14.92 ± 4.707). Fibroscan values decreased at SVR (17.596 ± 3.7276; p = 0.000) compared to baseline (26.068 ± 7.0954). For all common coagulation parameters (platelets, INR, PT, fibrinogen, aPTT) there was a trend towards improvement during treatment, changes that were statistically significant for the majority of patients. Factor II low at baseline (75.40 ± 7.506), increases at EOT (87.40 ± 9.587), and later at SVR (99.12 ± 11.695; p = 0.000). FVIII values increased at baseline (175.52 ± 16.414) decrease at EOT (151.48 ± 13.703) and SVR (143.40 ± 13.937). FvW values decreased during treatment (146.84 ± 9.428 – baseline, 141.32 ± 9.690; p =0.000 – EOT, 126.68 ± 17.960 – SVR). In regards to the anticoagulant factors (PC, PS, ATIII), a significant improvement was brought on by SVR. Advanced stages of liver disease showed the most diminished FII activity, while at baseline and in Child-Pugh C patients we recorded the highest values of FVIII and FvW. Conclusions: Our study proved that the “reset” of the coagulopathy might be due to the improving of the liver function due to viral eradication secondary to AAD therapy.

Preprint ARTICLE | doi:10.20944/preprints202409.0578.v1

Point of Common Connection Voltage Modulated Direct Power Control with Disturbance Observer to Increase of Renewable Energy Acceptance in Power System

Yong Woo Jeong, Woo Young Choi

Subject: Engineering, Electrical And Electronic Engineering Keywords: Grid voltage modulated power control; Point of common connection; Energy storage system; Voltage source inverter; Disturbance observer; Total harmonic distortion

Online: 9 September 2024 (08:00:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2334.v1

Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes

Hiroshi Yamada, Hirona Osaka, Nanami Tatsumi, Miu Araki, Tadashi Abe, Keiko Kaihara, Ken Takahashi, Eizo Takashima, Takayuki Uchihashi, Keiji Naruse, Kohji Takei

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SYNPO2L; actinin; actin; sarcomere; cardiomyocyte

Online: 29 July 2024 (16:21:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1390.v1

Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection

Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: droplet Digital PCR; canine distemper virus; molecular diagnosis

Online: 18 September 2024 (10:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1177.v1

A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis

Thaís Ellen Chaves Gomes, Victor Coutinho Bastos, Douglas Boniek, Mário Romañach, Fernanda Faria Rocha, Roberta Rayra Martins Chaves, Ricardo Santiago Gomez

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Aspergillosis; Aspergillus; PCR; Diagnosis

Online: 15 August 2024 (14:30:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0326.v1

Molecular Characterization of Termitomyces Protoclones through PCR and RFLP Based DNA Typing

Pijush Mallick, Sobhan Roy Chowdhury, Dipanwita Roy, Subhash Kanti Roy, Samir Ranjan Sikdar

Subject: Biology And Life Sciences, Horticulture Keywords: DNA markers; edible mushroom; genome stability; protoplast regeneration

Online: 25 April 2018 (08:26:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0371.v1

Rupestonic Acid Derivative YZH-106 Promotes Lysosomal Degradation of HBV L- and M-HBsAg via Direct Interaction with PreS2 Domain

Lanlan Liu, Haoyu Wang, Lulu Liu, Fang Cheng, Haji Akber Aisa, Changfei Li, Songdong Meng

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: YZH-106; HBV; HBsAg; degradation

Online: 7 May 2024 (08:56:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1477.v1

General Direct Anticancer Effects of Growing Antler Extract in Several Tumour Cell Lines, and Immune System-Mediated Effects in Xenograft Glioblastoma

Alessandra Rossetti, Louis Chonco, Nicolás Alegría, Veronica Veronica, Andrés J. García, Carmen Ramírez-Castillejo, Alessandra Tessitore, Carlos De Cabo, Tomás Landete-Castillejos, Claudio Festuccia

Subject: Biology And Life Sciences, Other Keywords: Anti-tumour activity; Cell proliferation; Deer Velvet Antler; Glioblastoma Multiforme; Immune tolerance

Online: 25 March 2024 (11:19:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0226.v1

Design and Analysis of a Novel Non-isolated DC-DC Modular Multilevel Converter Architecture for High Voltage Direct Current Power Networks Interconnections

Abdurrahim Erat, Ahmet Mete Vural

Subject: Engineering, Electrical And Electronic Engineering Keywords: HVDC; DC-DC Power Conversion; MMC; DC-DC MMC; Asynchronous Power Grids

Online: 2 April 2024 (15:49:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201610.0081.v1

Development and Application of a Wireless Sensor for Space Charge Density Measurement in an Ultra-High-Voltage, Direct-Current Environment

Encheng Xin, Yong Ju, Haiwen Yuan

Subject: Engineering, Control And Systems Engineering Keywords: Ultra High-voltage direct-current (UHVDC); space charge density; energy consumption; wireless communication; Zigbee

Online: 20 October 2016 (06:02:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0075.v1

Waste Plastic Direct Extrusion Hangprinter

Aliaksei Petsiuk, Bharath Lavu, Rachel Dick, Joshua M. Pearce

Subject: Engineering, Mechanical Engineering Keywords: 3D printing; additive manufacturing; big area additive manufacturing; BAAM; hanging printer; hangprinter; plastic waste; recycling; sustainable manufacturing; wire robot

Online: 3 August 2022 (07:17:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1323.v1

The Long-Term Effect of Mourning during the COVID-19 Pandemic: How Were People Affected by Direct Losses and Media Exposure to Death?

Barbara Caci, Giulia Giordano

Subject: Social Sciences, Psychology Keywords: COVID-19 pandemic; mental health; anxiety; depression; stress; mourning; vicarious grief; media exposure; neuroticism

Online: 19 June 2024 (16:35:15 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202204.0104.v1

Statistical Modelling and Experimental Design for the Validation of Droplet Digital PCR Methods

Steffen Uhlig, Christopher Weidner, Bertrand Colson

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Method validation; droplet digital PCR; orthogonal factorial design; variance components; Poisson assumption; cloglog model; target DNA copies per droplet; Monte Carlo; prediction interval

Online: 12 April 2022 (08:46:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0279.v1

COVID19 PCR Testing and the Unprecedented Institutional Response in a Low-Resource Setting

Akintunde Orunmuyi, Olukemi Adekanmbi, Adeola Fowotade, Akindele Adebiyi, Adekunle Adeniji, Olukemi Amodu, E. Oluwabunmi Olapade-Olaopa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID19; sub-Sahara Africa; PCR testing; Capacity building

Online: 13 July 2020 (06:32:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1390.v1

Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

Mirko Sonntag, Vanessa K. Elgeti, Yevhen Vainshtein, Lucca Jenner, Jan Mueller, Thorsten Brenner, Sebastian O. Decker, Kai Sohn

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sepsis; antimicrobial resistances; cell-free DNA; precision diagnostics; Next-Generation sequencing; suppression PCR; real-time diagnostics; nanopore sequencing

Online: 22 April 2024 (10:13:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1505.v1

Diagnostic Accuracy of Multiplex PCR in Early Onset Neonatal Sepsis

Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: neonatal sepsis; early onset sepsis; multiplex PCR; infection; neonate; diagnosis; pathogen detection

Online: 24 October 2023 (08:11:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0632.v1

Analysis of the Direct Costs of Neuromyelitis Optica in a Brazilian Hospital

Roberto Carlos Lyra da Silva, Daniel Aragão Machado, Carlos Roberto Lyra da Silva, Helena Andrade Figueira, Débora Viana Freitas, and Claudia Cristina Ferreira Vasconcelos

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: neuromyelitis optica; measure cost; Unified Health System.

Online: 10 May 2024 (10:38:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0435.v1

Evaluation of CO₂/H₂O Co-adsorption Models for the Anion Exchange Resin Lewatit Vpoc 1065 under Direct Air Capture Conditions Using a Novel Lab Setup

Florian Maximilian Chimani, Aditya Anil Bhandari, Andreas Wallmüller, Gerhard Schöny, Stefan Müller, Josef Fuchs

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: Direct air capture; CO₂ adsorption; Co-adsorption; Isotherm modeling; Negative emissions technology

Online: 8 May 2024 (15:17:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0268.v2

Difference Between Franchisers with and without Direct Retail Stores

Jin Hyung Lee

Subject: Business, Economics And Management, Economics Keywords: franchise market; market saturation; regulation

Online: 11 March 2024 (11:03:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0425.v1

Direct Link Between Energy and Information via Fisher’s Measure

F. Pennini, Angelo Plastino

Subject: Physical Sciences, Mathematical Physics Keywords: Fisher information; Poisson distribution; energy variance

Online: 7 February 2024 (08:12:48 CET)

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Preprint REVIEW | doi:10.20944/preprints201809.0084.v1

Company Income Taxes and Foreign Direct Investment in Nigeria

Onome Edo, Omoye Alade

Subject: Business, Economics And Management, Economics Keywords: corporate tax; fdi; tax incentives; tax rate; investment

Online: 5 September 2018 (05:10:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Biology And Life Sciences, Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2272.v1

The Hypocholesterolemic Potential of the Edible Algae Fucus Vesiculosus: Proteomic and Quantitative PCR Analysis

Rebeca André, Rita Pacheco, Ana Catarina Alves, Hugo M. Santos, Mafalda Bourbon, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Fucus vesiculosus; cholesterol synthesis; cholesterol excretion; , Ezetimibe; , NPC1L1; ABCG5; qRT-PCR; mRNA

Online: 31 May 2023 (14:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0196.v1

SARS-CoV-2 PCR Persistence and Duration of COVID-19 Symptoms in Health Professionals

Tatiana Schäffer Gregianini, Ludmila Fiorenzano Baethgen, Richard Steiner Salvato, Ivone Andreatta Menegolla, CEVS COVID-19 Team, Ana Beatriz Gorini da Veiga

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: SARS-CoV-2; PCR persistent positive; respiratory infection; COVID-19

Online: 12 December 2022 (09:04:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0577.v1

Effect of Total Nitrogen Once Applied at Different Leaf Age Stages on Yield Traits of Dense Planted Dry Direct Seeding Rice

Zhipeng Xing, Dihui Fu, Jie Zhao, Feiyang Chen, Minmin Sun, Hongcheng Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: nitrogen; applied time; leaf age; dense planted; yield; direct seeding rice

Online: 9 September 2024 (07:01:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1582.v1

An Overview of the Arguments in Literature, For and Against the Use of Green Hydrogen for the Production of Direct Reduced Iron

Göran Roos

Subject: Business, Economics And Management, Business And Management Keywords: Green hydrogen; Direct Reduced Iron (DRI); Decarbonisation; Steel industry; Carbon Capture and Storage (CCS); Renewable energy; Electrolysis; Energy efficiency; Geopolitical implications; Social acceptance

Online: 23 May 2024 (16:45:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0581.v1

Looking into the Quantification of Forensic Samples with Real-Time PCR

Ugo Ricci, Dario Ciappi, Ilaria Carboni, Claudia Centrone, Irene Giotti, Martina Petti, Alice Brogi, Elisabetta Pelo

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: DNA amplification; polymerase chain reaction; DNA quantification; forensic DNA

Online: 9 May 2024 (10:39:14 CEST)

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The quantification of human DNA extracts from forensic samples plays a key role in the forensic genetics process. Quantification guarantees maximum efficiency and avoids repeated analyses, over amplified samples, or unnecessary examinations. Here, we present our experience using an extremely sensitive real-time-based method with the Quantifiler® Trio DNA quantification kit, which we have explored in the ISO/IEC17025 accredited test validation process conducted in our laboratory. In this study, our focus was on establishing a minimum threshold beyond which DNA typing is unnecessary, by defining the correlation of negative quantification results with the absence of genetic profiles resulting from DNA typing. We investigated the results in the range in which the technique is used (50-0.005 ng/μl). In the validation process of the internally accredited method, we used data from the commercial quantification system, based on the average values of the standard curve using a wide range of samples, including artificially degraded samples. This method was extensively used continuously for nine years to quantify the DNA extract coming from a wide range of samples from criminal cases. The validation allowed us to verify the limits of the technique, especially in the use of the minimum quantities necessary to obtain genetic profiles acceptable for sending to the Italian DNA database. This optimization of the protocol for analysing forensic samples allows us to optimise the protocol for analysing forensic samples. However, the technique is used in our laboratory with a completely manual preparative phase, and we have decided to investigate the contribution of human error more accurately. Thus, in our experience, we have found that it is preferable to use quantification ranges rather than exact limits before deciding how to analyse the extracts via PCR. In addition, here we present some preliminary data related to the analysis of certain samples that initially appeared negative during quantification, applying a concentration method for maximum yield from forensic samples of judicial interest. Our aim is to ensure that no source of evidence is lost in the reconstruction of a criminal event.

Preprint ARTICLE | doi:10.20944/preprints202111.0210.v1

Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System

Shang Wang, Ying Tang, Jiehong Wei, Shan Wang, Guoyu Peng, Yuqing Li, Song Wu

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: CRISPR-Cas, Nucleic acid detection, Klebsiella pneumonia

Online: 11 November 2021 (12:57:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0189.v1

Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification

Lorena G. Caligiuri, Adolfo E. Sandoval, Jose C. Miranda, Felipe A. Pessoa, María S. Santini, Oscar D. Salomón, Nagila F. C. Secundino, Christina McCarthy

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sand fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia

Online: 17 December 2018 (10:41:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0753.v1

The Effects of Foreign Direct Investments on Wage Trends in the Dutch Labor Market

Sergiu Ionel Pirju, Sirbu Carmen, Ioan Gina, Angelica Nicoleta Neculaesei Onea, Emanuel Stefan Marinescu, Rodica Pripoaie

Subject: Business, Economics And Management, Economics Keywords: FDIs; Development; Dutch economy; wages

Online: 10 September 2024 (09:00:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1144.v1

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Gabriel S.M. Sousa, Rodrigo S. De Oliveira, Alex B. De Souza, Ruan C. Monteiro, Elaine P. T. E. Santo, Luciano C. Franco Filho, Denison L.O. Moraes, Sarah R. De Sá, Silvia H. M. Da Silva

Subject: Biology And Life Sciences, Other Keywords: chromoblastomycosis; phaeohyphomycosis; PCR-Multiplex; Herpotrichiellaceae; sequencing; phenotypic; molecular biology.

Online: 19 March 2024 (12:35:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1348.v1

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Pallavi Upadhyay, Jairus Reddy, Teddie Proctor, Oceane Sorel, Harita Veereshlingam, Manoj Gandhi, Vijay Singh

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Respiratory tract infections; COVID-19; influenza-like illness; multiplex PCR; syndromic panels

Online: 18 May 2023 (14:10:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0991.v1

Potential Performance of Two New RT-PCR and RT-q PCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes Allowing Efficient Entomological Surveillance of Both Vector-Borne Diseases

Michel Kiréopori Gomgnimbou, Louis robert wendyam BELEM, Etienne Bilgo, Miriam Félicité Amara, Zouera Laouali, Ali Ouari, Toussaint Bayala, Kobo Gnada, Raymond Kharlis Yao, Moussa Namountougou, Ibrahim Sangaré

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: multiplex rt-pcr; multiplex rt-qpcr; molecular detection; dengue virus; chikungunya virus; mosquitoes; Burkina Faso

Online: 12 July 2024 (08:43:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0563.v1

Industrial Scale Direct Liquefaction of Eucalyptus Biomass

Irina Fernandes, José Condenço, Duarte Cecilio, Maria Joana Neiva Correia, João Carlos Moura Bordado, Margarida Mateus

Subject: Engineering, Chemical Engineering Keywords: Energreen; Biomass liquefaction; Pilot Scale; Bio-oils; Sugars extraction; Stabilization

Online: 8 August 2023 (03:41:08 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202111.0549.v1

PCR, PLS, or OPLS Evaluation of different regression techniques for hypothesis generation

Avani Ahuja

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Principal Component Regression, Partial Least Squares, Orthogonal Partial Least Squares, multivariate regression, hypothesis generation, Parkinson’s disease

Online: 29 November 2021 (15:42:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1348.v1

Direct Imaging of Radiation Sensitive Organic Polymer-Based Nanocrystals at Sub-Ångström Resolution

Elvio Carlino, Antonietta Taurino, Dritan Hasa, Dejan-Krešimir Bučar, Maurizio Polentarutti, Lidia Esther Chincilla, Jose Juan Calvino Gamez

Subject: Chemistry And Materials Science, Other Keywords: Radiation damage; Polymers; soft matter; HoloTEM; atomic Resolution Imaging; in-line Holography; HRTEM

Online: 22 April 2024 (12:25:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202301.0084.v1

Microbial Evolution: The Dilemma of Direct and Indirect Viral Self-Camouflaging

Theodor-Nicolae Carp

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: microbial evolution; immune evasion; first-line immunity; non-structural protein; interferon; lymphocytes; metabolism; ageing

Online: 4 January 2023 (12:43:27 CET)

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Microbial immune escape represents the primary cause of induced pathogenesis in humans, and it represents a pivotal method used by viral agents to increase their load and suppress key mechanisms of the innate and adaptive immune system. This phenomenon represents the primary factor that led to the onset of the 1918-1920 A(H1N1) Influenza and 2020-2022 COVID-19 pandemics, and it possibly played a major role in the onset of the AIDS pandemic as well. Moreover, repeated incidents of immune evasion could be associated with higher rates of cellular aging (Jackson et al., 2017), most likely due to the consequent increased demands of energy consumption. Highly developed viral immune evasion ultimately indicates the high inner intelligence of human immunity due to reflective and imitative characteristics of reactions that are produced against initial actions. Ribonucleic acid-based viral genomes contain open reading frames, which consist of genes producing sixteen non-structural proteins. Such proteins play a considerable role in desensitizing first-line immunity during cellular infection, and non-structural proteins 1, 10 and 16 have the strongest effects against a healthy expression rate of Type I and Type III Interferon-encoding genes. Type I Interferons consist of IFN-alpha, -beta, -delta, -epsilon, -omega, -tau and -zeta, whilst Type III Interferons consist of IFN-lambda1, -lambda2 and -lambda3, and they act as stimulators of intracellular signalling cascades that in turn lead to the activation and expression of interferon-stimulated genes (Brown et al., 2022). The earlier the interferon-stimulated genes are activated, the lower the extent of pro-inflammatory mediation and overall, the more effective the antiviral immune response will be, given the exponential nature of the viral load increase. Non-structural protein 16 methylates the 5’ cap of the virus, making the pathogen-associated molecular patterns less recognisable by pattern-recognition receptors, and it requires activation by bonding with non-structural protein 10. It is preserved in the S-Adenosyl-L-Methionine pocket of the SARS-CoV-2 genome. Non-structural protein 1 (NS1) directly cleaves the host cell mRNA producing Type I and possibly Type III Interferons, thereby preventing a translation process of the immune proteins. NS1 has recently been found to often be packaged into exosomes once secreted by the viral genome in the cytosol, meaning that exocytosis and paracrine signalling to neighbouring cells before their actual infection is possible. As a result, NS1 is highly capable of silencing the first-line immune responses of uninfected neighbouring cells as well, thereby highlighting the need to adjust the focus of therapeutics and vaccinology toward first-line immunity and further indicating its foundational importance in the support for the development of precise and balanced defenses against microbial agents of concern (EL SAFADI et al., 2022).

Preprint COMMUNICATION | doi:10.20944/preprints202306.2007.v1

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega, Klaudia Babisova, Michaela Hyblova, Nikola Janostiakova, Peter Sabaka, Pavol Janega, Gabriel Minárik

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Online: 29 June 2023 (02:12:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint ARTICLE | doi:10.20944/preprints202308.0284.v1

Designing and Validation of a Droplet Digital PCR Procedure for Diagnosis and Accurate Quantification of Nervous Necrosis Virus

Sandra Souto, José G. Olveira, Carmen López-Vázquez, Isabel Bandín, Carlos P. Dopazo

Subject: Biology And Life Sciences, Virology Keywords: VNNV; Diagnosis; Validation; dPCR; droplet digital PCR.

Online: 3 August 2023 (10:44:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0212.v1

One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3

Xueming Chen, Yuhuan Chen, Chunguo Liu, Xiaojun Li, Hongyu Liu, Xiaofei Bai, Ming Liu, Yun Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DHAV-1; DHAV-3; Phylogenetic analysis; One-tube RT-PCR; Simultaneously

Online: 22 January 2019 (11:08:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.1225.v1

Exploration of Temperature-Induced Quantum Proton Tunneling Probability in PCR-Mediated DNA Amplification

Joseph De Luna

Subject: Physical Sciences, Biophysics Keywords: genetics; molecular biology; biophysics; quantum mechanics

Online: 19 September 2023 (05:18:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1033.v2

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Meynard R. Austria, Jon Patrick T. Garcia, Lemmuel L. Tayo, Alvin R. Caparanga, Bonifacio Jr T. Doma

Subject: Engineering, Bioengineering Keywords: polymerase chain reaction (PCR); Computational Fluid Dynamics (CFD); Plasmodium falciparum; microfluidic chip design

Online: 10 August 2023 (10:30:52 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202211.0436.v1

Multiplex PCR Detects Respiratory Viruses at A Much Higher Rate in Kawasaki Disease

Masayuki Nagasawa, Teruyoshi Shimoyama, Sayuri Hashimoto, Ryuichi Nakagawa, Haruna Yokoyama, Mari Okada, Tomohiro Udagawa, Akihiro Oshiba

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Kawasaki disease; febrile convulsions; respiratory virus; filmarray; multiplex PCR

Online: 23 November 2022 (07:02:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.1088.v1

Chitosan Membranes for Direct Methanol Fuel Cell Applications

Fulufhelo Nemavhola, Livhuwani Modau, Rudzani Sigwadi, Mouhumi Mokrani

Subject: Engineering, Chemical Engineering Keywords: Chitosan; Fuel Cell; Water Uptake; Proton Conductivity; Silica, Sulfonated

Online: 17 July 2023 (09:19:49 CEST)

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Working Paper ARTICLE

Spectral Data of Conformable Sturm-Liouville Direct Problems

Erdal Bas, Funda Metin Turk, Ramazan Ozarslan, Ahu Ercan

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Sturm-Liouville; conformable derivative; asymptotic formula; spectral data

Online: 25 December 2019 (03:13:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0119.v1

Processes of Direct Laser Writing 3D Nano-Lithography

Simonas Varapnickas, Mangirdas Malinauskas

Subject: Physical Sciences, Applied Physics Keywords: 3D nano-lithography, 3D laser lithography, direct laser writing, nanopolymerization, cross-linking, multi-photon absorption, avalanche ionization, temperature effects

Online: 11 December 2018 (09:31:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.2169.v1

Spleen Swabs for Sensitive and High-Throughput Detection of African Swine Fever Virus by Real-Time PCR

Christopher Cafariello, Kalhari Goonewardene, Chungwon J Chung, Aruna Ambagala

Subject: Biology And Life Sciences, Virology Keywords: African swine fever; Diagnostics; Tissue homogenate; Spleen swab; Real-time PCR

Online: 26 July 2024 (16:49:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0184.v1

Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Result in a Decrease in PCR Efficiency

Olga A. Zasedateleva, Sergey A. Surzhikov, Viktoriya E. Kuznetsova, Valeriy E. Shershov, Victor E. Barsky, Alexander S. Zasedatelev, Alexander V. Chudinov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: C5-modified dUTPs; A and B family DNA polymerases; PCR amplification; X-ray structure; molecular modeling; non-covalent interactions

Online: 2 August 2023 (08:21:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1920.v1

Selection and Evaluation of Reference Genes for qRT-PCR Analysis in Amorphophallus konjac Based on Transcriptome Data

Yanli Liu, Chengcheng Zhang, Nunung Harijati, Ying Diao, Erxi Liu, Zhongli Hu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Amorphophallus; reference genes; RT-qPCR; gene expression

Online: 27 June 2023 (13:43:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0091.v1

Evidence-Based Evaluation of PCR Diagnostics for SARS-Cov-2 and The Omicron Variants by Sanger Sequencing

Sin Hang Lee

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: SARS-CoV-2; SARS-CoV; RT-PCR; Sanger sequencing; RT-qPCR; receptor-binding domain (RBD); N-terminal domain (NTD); Omicron; multi-allelic SNPs; false-positive

Online: 12 April 2022 (03:57:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0897.v1

Revisiting Maxwell's Equations for Observers: Direct Derivation of Doppler and Sagnac Effect

Qian Chen

Subject: Physical Sciences, Optics And Photonics Keywords: Maxwell equations; Doppler effect; Doppler radar; Electromagnetic propagation; Wave functions; Sagnac effect

Online: 13 August 2024 (08:46:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0038.v1

Direct Bonding Method for Completely Cured Polyimide by Surface Activation and Wetting

Ying Meng, Runhua Gao, Xinhua Wang, Sen Huang, Ke Wei, Dahai Wang, Fengwen Mu, Xinyu Liu

Subject: Engineering, Electrical And Electronic Engineering Keywords: polyimide bonding; plasma activation; hydrophilic; hybrid bonding; 3D integration

Online: 2 March 2022 (07:47:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints201911.0262.v1

On a Dual Direct Cosine Simplex Type Algorithm and Its Computational Behavior

Elsayed Badr, khalid Aloufi

Subject: Computer Science And Mathematics, Computational Mathematics Keywords: linear programming; dual simplex method; dual direct cosine method; two-phase method

Online: 22 November 2019 (10:06:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0061.v1

Handheld Ultra-Fast duplex PCR assays and Lateral flow detection and identification of Leishmania parasites for cutaneous leishmanisis diagnosis

Insaf Bel Hadj Ali, Yusr Saadi Ben Aoun, Zeineb Hammami, Oumayma Rhouma, Ahmed Sahbi Chakroun, Ikram Guizani

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Cutaneous leishmaniases; Molecular diagnosis; Point of care; Leishmania; Molecular target; Palm PCR; Duplex PCR; Lateral Flow immunoassay

Online: 2 October 2023 (09:43:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.2000.v1

A Simple Inexpensive Alkaline Method for DNA Extraction from Environmental Samples for PCR Surveillance

Abdulkarim Shwani, Bin Zuo, Adnan Alrubaye, Jiangchao Zhao, Douglas Duane Rhoads

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: microbiome; bacteria; DNA extraction; poultry; swine; air sampling

Online: 30 November 2023 (15:40:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints201912.0173.v1

An Efficient and Leightweight Illumination model for Planetary Bodies including Direct and Diffuse Radiation

Marco Scharringhausen

Subject: Computer Science And Mathematics, Computational Mathematics Keywords: illumination; surface properties; planetary imaging

Online: 12 December 2019 (12:32:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1939.v1

Actinobacillus pleuropneumoniae Serotypes by Multiplex PCR Identification and Evaluation of Lung Lesions in Pigs from Piedmont (Italy) Farms

Matteo Cuccato, Sara Divari, Silvia Ciaramita, Alessandra Sereno, Domenico Campelli, Pier Giuseppe Biolatti, Bartolomeo Biolatti, Francesco Meliota, Enrico Bollo, Francesca Tiziana Cannizzo

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Actinobacillus pleuropneumoniae; porcine pleuropneumonia; swine; respiratory diseases; serotyping; multiplex PCR

Online: 29 May 2024 (14:03:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0571.v1

Streamlined Two-Step Fragment Analysis PCR and Exome Sequencing of RFC1 for Diagnostic Testing of Suspected CANVAS Patients

Helena Jaklič, Ivana Babič Božović, Borut Peterlin, Anja Kovanda

Subject: Medicine And Pharmacology, Other Keywords: Cerebellar ataxia neuronopathy and vestibular areflexia syndrome (CANVAS); RFC1; genetic testing; genetic diagnostics, ; fluorescent PCR; repeat-primed PCR; repeat-expansion; fragment length analysis

Online: 11 March 2024 (05:36:41 CET)

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Working Paper ARTICLE

Detection of Peanut Allergen by Real Time PCR: Looking For the Suitable Detection Marker as Affected by Processing

Africa Sanchiz, Paulina Sánchez-Enciso, Carmen Cuadrado, Rosario Linacero

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Real-time PCR; peanut; food allergen; chloroplast marker; DNA isolation

Online: 16 June 2021 (11:33:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1054.v1

Direct Current To Digital Converter (DCDC): A Current Sensor

Saeid Karimpour, Michael Sekyere, Isaac Bruce, Emmanuel Nti Darko, Degang Chen, Colin C. McAndrew, Doug Garrity, Xiankun Jin, Ilhan Hatirnaz, Chen He

Subject: Engineering, Electrical And Electronic Engineering Keywords: reliability; measurement; ADC; DCDC; CMOS; VLSI; SAR

Online: 13 September 2024 (08:25:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0376.v1

Turbulent Channel Flow: Direct Numerical Simulation-Data-Driven Modeling

Antonios Liakopoulos, Apostolos Palasis

Subject: Engineering, Mechanical Engineering Keywords: Wall-bounded turbulence; turbulent boundary layers; higher order statistics; Direct Numerical Simulation (DNS)

Online: 7 February 2024 (02:11:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0138.v1

Phage vs Phage: Direct Selections of Sandwich Binding Pairs

Emily C Sanders, Alicia M Santos, Eugene K Nguyen, Aidan A Gelston, Sudipta Majumdar, Gregory A Weiss

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: phage display; virus selections; sandwich binding; diagnostic assays; non-covalent binding

Online: 8 February 2023 (04:42:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202007.0394.v1

Direct Measurement of Temperature Diffusivity of Nanocellulose Composite Films

Hiroki Fujisawa, Meguya Ryu, Stefan Lundgaard, Denver Linklater, Elena Ivanova, Yoshiaki Nishijima, Saulius Juodkazis, Junko Morikawa

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: thermal properties; temperature diffusivity; nano cellulose

Online: 17 July 2020 (15:36:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202004.0300.v1

Direct Transformation of Crystalline MoO₃ into Few-Layers MoS₂

Felix Carrascoso, Gabriel Sanchez-Santolino, Chun-wei Hsu, Norbert M. Nemes, Almudena Torres-Pardo, Patricia Gant, Federico J. Mompean, Kourosh Kalantar-zadeh, Jose A. Alonso, Mar Garcian-Hernandez, Riccardo Frisenda, Andres Castellanos-Gomez

Subject: Chemistry And Materials Science, Nanotechnology Keywords: 2D materials; molybdenum trioxide (MoO3); molybdenum disulfide (MoS2); synthesis; sulfuration

Online: 17 April 2020 (13:07:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.2128.v1

Microstructure and Mechanical Properties of Laser Direct Energy Deposited Martensitic Stainless Steel 410

Hyun-KI KANG, Hyungsoo Lee, Chang-Seok Oh, Jongcheon Yoon

Subject: Engineering, Metallurgy And Metallurgical Engineering Keywords: Additive manufacturing; Directed energy deposition; Martensitic Stainless steel 410; Chrome car-bide; Tempering

Online: 31 May 2024 (10:56:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1237.v1

The Direct Tendon Suture and Paratenon Repair Technique for Acute Tendinous Mallet Finger

Seungjun Lee, Seokchan Eun

Subject: Medicine And Pharmacology, Surgery Keywords: tendon injuries; mallet finger; suture techniques

Online: 18 April 2024 (10:34:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.2023.v1

Experimental Investigation and Mechanism Analysis of Direct Aqueous Mineral Carbonation Using Steel Slag

Fuxia Zhu, Longpeng Cui, Yanfang Liu, Liang Zou, Jili Hou, Chenghao Li, Ge Wu, Run Xu, Bo Jiang, Zhiqiang Wang

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: Steel slag; Direct aqueous carbonation; CO2 sequestration; Parameter optimization; Mechanism analysis

Online: 31 October 2023 (09:06:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1116.v1

Construction of Supplemental Functions for Direct Serendipity and Mixed Finite Elements on Polygons

Todd Arbogast, Chuning Wang

Subject: Computer Science And Mathematics, Computational Mathematics Keywords: serendipity finite elements; direct finite elements; optimal approximation; polygonal meshes; finite element exterior calculus

Online: 18 October 2023 (09:34:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202106.0096.v1

Comparison of Direct Intersection and Sonogram Methods for Acoustic Indoor Localization of Persons

Dominik Schott, Addythia Saphala, Georg Fischer, Wenxin Xiong, Andrea Gabbrielli, Joan Bordoy, Fabian Höflinger, Kai Fischer, Christian Schindelhauer, Stefan Johann Rupitsch

Subject: Engineering, Automotive Engineering Keywords: Presence detection; passive localization; room impulse response; acoustic localization; indoor localization

Online: 3 June 2021 (09:57:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1487.v1

Simultaneous Detection of Three Subgroups of Avian Leukosis Virus Using the Nanoparticle-Assisted PCR Assay

Miaoli Wu, Shuaiqi Hu, Yujun Zhu, Feng Cong, Shengwang Liu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: nanoPCR; avian leukosis virus; ALV-J; detection

Online: 22 September 2023 (04:33:14 CEST)

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Preprint SHORT NOTE | doi:10.20944/preprints202306.1658.v1

Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry

Wenfa Ng

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: qPCR assay; probe-based detection; SYBR green; human KRAS gene; human gastric CloTest samples

Online: 23 June 2023 (10:50:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202010.0379.v1

Evaluation of Reference Genes for qRT-PCR Normalization in Angelica decursiva under Various Experimental Conditions

Yuedong He, Yuan Zhong, Zhenzhen Bao, Weiqi Wang, Xiaoqing Xu, Yanan Gai, Jie Wu

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: qRT-PCR; reference gene; Angelica decursiva; expression stability

Online: 19 October 2020 (12:25:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1094.v1

Development and Evaluation of an In-House Real-Time Rt-Pcr Targeting nsp10 Gene for SARS-CoV-2 Detection

Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; nsp10; real-time RT-PCR; diagnostic

Online: 20 February 2024 (06:07:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202005.0440.v1

Clinical Performance Analysis and Evaluation of Quantitative Real Time PCR for Diagnosis of Scrub Typhus in North East India

Diptanu Paul, W Valarie Lyngdoh, Himesh Barman, Clarissa Jane Lyngdoh, Kryshanlang Lynrah, Elantamilan Durairaj

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Scrub typhus; qRT PCR; Quantitative PCR; real time PCR; IgM ELISA; North-East India

Online: 27 May 2020 (07:51:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202103.0109.v1

Identification and Biotyping of Pythium insidiosum Isolated from Urban and Rural Areas of Thailand by Multiplex PCR, DNA Barcode, and Proteomic Analyses

Zin Mar Htun, Aree Laikul, Watcharapol Pathomsakulwong, Chompoonek Yurayart, Tassanee Lohnoo, Wanta Yingyong, Yothin Kumsang, Penpan Payattikul, Pattarana Sae-Chew, Thidarat Rujirawat, Paisan Jittorntam, Chalisa Jaturapaktrarak, Piriyaporn Chongtrakool, Theerapong Krajaejun

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Pythiosis; Pythium insidiosum; Environment isolate; DNA barcode; Biotyping

Online: 2 March 2021 (16:41:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.0797.v1

Direct Estimation of Equivalent Bioelectric Sources Based on Huygens Principle

Georgia Theodosiadou, Dimitrios G. Arnaoutoglou, Ioannis Nannis, Sotirios Katsimentes, Georgios Ch. Sirakoulis, George A. Kyriacou

Subject: Engineering, Bioengineering Keywords: (ECG); Huygens’ Principal; reciprocity theorem; finite element method

Online: 13 July 2023 (04:48:38 CEST)

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Preprint SHORT NOTE | doi:10.20944/preprints202105.0017.v1

Production of Hardened Body by Direct Bonding of Sand Particles

Yuya Sakai

Subject: Engineering, Automotive Engineering Keywords: Sand, Cement-Free, Catalyst

Online: 4 May 2021 (13:44:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0230.v1

Does Poverty deter Foreign Direct Investment flows to Developing Countries?

Sena Kimm Gnangnon

Subject: Business, Economics And Management, Accounting And Taxation Keywords: Poverty; Foreign direct investment inflows; Human capital; Trade openness; Export product diversification; Economic growth; Labour productivity; Financial development; Infrastructure development.

Online: 6 November 2020 (09:03:02 CET)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202003.0169.v1

Direct Ink Writing Glass: A Preliminary Step for Optical Application

Bo Nan, Przemysław Gołębiewski, Ryszard Buczyński, F.J. Galindo Rosales, José Ferreira

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: direct ink writing; glass; rheology

Online: 10 March 2020 (11:18:45 CET)

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