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Preprint ARTICLE | doi:10.20944/preprints202408.0976.v1

Computationally Rationalising the Conformational Dynamics of Pseudo-Endocannabinoid Receptors CB₁ and CB₂ with Cannabidiol

Ikechukwu Achilonu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Pseudo-Endocannabinoid Receptors; Cannabis; Cannabidiol; Computational Biology; Induced-Fit Docking; Molecular Dynamic Simulations

Online: 16 August 2024 (03:05:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1872.v1

Novel protein-rich materials from rapeseed meal with enhanced mechanical behavior

Sara Aquilia, Luca Rosi, Michele Pinna, Sabrina Bianchi, Walter Giurlani, Marco Bonechi, Francesco Ciardelli, Anna Maria Papini, Claudia Bello

Subject: Chemistry And Materials Science, Biomaterials Keywords: Rapeseed meal-based material; Protein-based biopolymers; Renewable biomaterials.

Online: 27 June 2024 (08:08:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0127.v1

Exploring the Efficacy of Alpha-Lipoic Acid in Comorbid Osteoarthritis and Type 2 Diabetes Mellitus

Iryna Halabitska, Valentyn Oksenych, Oleksandr Kamyshnyi

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: osteoarthritis; type 2 diabetes; diabetes mellitus; alpha-lipoic acid; inflammation; oxidative stress; glycemic control; comorbidity

Online: 2 September 2024 (23:34:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0766.v1

Antibody Recognition to the Hyperglucosylated Adhesin Protein HMW1ct of Non Typeable Haemophilus Influenzae in Rett Syndrome

Feliciana Real Fernandez, Francesca Nuti, Lorenzo Altamore, Mattan Hurevich, Nir Qvit, Eduardo Ponticiello, Paolo Rovero, Joussef Hayek, Anna Maria Papini

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Adhesin protein; N-glucosylation; Antibodies; Immunoenzymatic assay; Rett syndrome ;

Online: 10 July 2024 (04:37:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0441.v1

Overcoming CD Spectroscopy Sample Preparation Challenges in Protein Structural Analysis for Biosimilars

Temitope Agbelusi, Ketan Pancholi, Harini Sampatk Kumar, Amit Kumar

Subject: Medicine And Pharmacology, Pharmacy Keywords: protein; surfactant; SDS; protein-surfactant-interaction; circular-diachorism; Chirality

Online: 5 January 2024 (10:38:20 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.1386.v2

Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis

Catherine Vénien-Bryan, Carlos A. H. Fernandes

Subject: Biology And Life Sciences, Biophysics Keywords: membrane proteins; cryo-electron microscopy; detergents; nanodiscs; amphipols; structural biology

Online: 22 September 2023 (13:10:51 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202406.1617.v1

Recombinant C-terminal Catalytic Domain of Rat L-gulono Lactone Oxidase Produced in Bacterial Cells Is Enzymatically Active

Abdul Aziz Mohamed Gad, Anna Gora-Sochacka, Agnieszka Sirko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Gulonolactone oxidase; recombinant protein; GULO activity; His-tag

Online: 24 June 2024 (08:44:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0792.v1

Synthesis of Shaker Input Signals with High Kurtosis for Vibration Qualification Testing

Marco Troncossi, Emanuele Pesaresi, Alessandro Rivola

Subject: Engineering, Mechanical Engineering Keywords: vibration qualification testing; random vibration; non-gaussian signals; kurtosis control; test tai-loring; mission synthesis

Online: 13 September 2023 (02:54:29 CEST)

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Working Paper ARTICLE

Improving the Quality Conduct and Efficiency of Clinical Trials with Training: Recommendations for Qualification of Investigators and Delegates

Jimmy Bechtel, TIna Chuck, Annemarie Forrest, Christine Hildebrand, Janette Panhuis, Suzanne R. Pattee, Sabrina Comic-Savic, Teresa Swezey

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: clinical trials; investigator qualification; good clinical practice; investigator training

Online: 8 October 2019 (05:18:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.1116.v1

Radioimmunotheragnosis in Cancer Research

Guillermo Garaulet, Barbara Baez, Guillermo Medrano, María Rivas-Sánchez, David Sánchez-Alonso, Jorge L. Martinez-Torrecuadrada, Francisca Mulero

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Theragnosis; Oncology; Nuclear Medicine; ImmunoPET; Radioimmunotherapy

Online: 17 June 2024 (08:53:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1255.v1

The Role of the Unbinding Cycle on the Coordination Abilities of the Bi-Cyclopeptides Toward Cu(II) Ions

Alicja Lisowska, Paulina Swiatek, Filip Debicki, Agniezska Lewinska, Aleksandra Marciniak, Lorenzo Pacini, Anna Maria Papini, Justyna Brasun

Subject: Chemistry And Materials Science, Other Keywords: bi-cyclopeptide; histidine; metal complex; copper ions; stability constant; spectroscopic studies

Online: 18 April 2024 (14:10:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201702.0052.v1

Simplified Swarm Optimization Based Function Modules Detection in Protein-to-Protein Interaction Networks

Xianghan Zheng, Lingting Wu, Shaozhen Ye, Riqing Chen

Subject: Computer Science And Mathematics, Information Systems Keywords: protein–protein interaction networks; protein function module; simplified swarm optimization

Online: 15 February 2017 (10:17:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202407.0325.v1

Inter-molecular Interactions of High Concentration Antibody Formulation Resulting in Problems of Viscosity, Opalescence and Phase Separation

Tsutomu Arakawa, Suguru Nishinami, Kentaro Shiraki, Yui Tomioka, Teruo Akuta

Subject: Biology And Life Sciences, Biophysics Keywords: arginine; antibodies; viscosity; opalescence; phase separation

Online: 3 July 2024 (08:51:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0894.v1

Anti-cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Model

Dina Yousif, Yan Wu, Alexandria Azul Gonzales, Christa Mathieu, Yan Zeng, Sample Lee, Sabrina Terando, Ting Li, Jin Xiao

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Saccharomyces cerevisiae; Saccharomyces boulardii; Candida albicans; cross-kingdom interaction; pH; Dental caries

Online: 11 January 2024 (08:04:48 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202405.0744.v1

Silica Gel Chromatographic Methods for Identification, Isolation and Purification of Gossypol Acetic Acids Prepared in Lab

Amro Abd Al Fattah Amara, Mohamed Hesham El-Masry, Gamal Ali Salem, Hoda Hassan Baghdadi

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Silica gel; Silica gel column; Robust spherical silica gel; Gossypol; Gossypol acetic acid; isolation; purification

Online: 13 May 2024 (07:46:10 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.1413.v1

Reactive Oxygen Species Mechanisms That Regulate Protein-Protein Interactions in Cancer

Stavros Iliadis, Nikolaos A Papanikolaou

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: ROS; PPI; cancer; degradation; phosphorylation; kinases; phosphatase

Online: 20 August 2024 (12:04:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0992.v1

Efficient Quality Control of Peptide Pools

Gaby Bosc-Bierne, Shireen Ewald, Oliver J. Kreuzer, Michael G. Weller

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: peptide pools; quality control; UHPLC-UV-HRMS; relative quantification; substance confirmation; structure; cost efficiency; CEF; infectious diseases; orbitrap

Online: 15 April 2024 (14:56:29 CEST)

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Abstract: Peptide pools composed of short amino acid sequences have proven to be versatile tools in various research areas and clinical applications. They are powerful tools for epitope mapping, immunotherapy, and vaccine development. Their importance lies in their ability to map complex protein structures, enabling a comprehensive understanding of immune responses and facilitating the identification of potential therapeutic agents. The application of peptide pools also extends to the field of personalized medicine, offering tailored solutions for diseases such as cancer and infectious diseases. Peptide pools are complex mixtures of immunostimulatory antigens primarily intended for T-cell stimulation. They are commercially available in many different compositions and variants. However, unlike other reagents that consist of only one or a few compounds, peptide pools are highly complex products with limited stability. This makes their quality control a major challenge. Quantitative peptide analysis usually requires sophisticated methods, in most cases isotope-labeled standards and reference materials. None of these are routinely available for these products. Synthesis and purification of all labeled peptides might be required. Usually, this approach would be prohibitively laborious and expensive. Therefore, an approach is needed to provide a practical and feasible method for quality control of peptide pools. With insufficient quality control, the use of such products could lead to incorrect experimental results, which would worsen the well-known reproducibility crisis in the biomedical sciences. Here we propose the use of ultra-high performance liquid chromatography (UHPLC) with two detectors, a standard UV detector (at 214 nm) for quantitative analysis and a high-resolution mass spectrometer (HRMS) for identity confirmation. To be cost-efficient and fast, quantification and identification are performed in one chromatographic run. An optimized protocol is shown, and different peak integration methods are compared and discussed. This work was performed using a peptide pool known as CEF, which consists of 32 peptides derived from cytomegalovirus (CMV), Epstein-Barr virus (EBV) and influenza virus, ranging from 8 to 12 amino acids in length.

Preprint ARTICLE | doi:10.20944/preprints202409.0376.v1

Evaluation of C-C Motif Chemokine Receptor 5 (CCR5) as a Sample Adequacy Control in HPV Molecular Diagnostics

Ruth Chinyere Njoku, Marianna Martinelli, Chiara Giubbi, Sofia De Marco, Barbara Torsello, Morena D'Avenia, Manuela Sironi, Cristina Bianchi, Clementina Elvezia Cocuzza

Subject: Biology And Life Sciences, Virology Keywords: C-C Motif Chemokine Receptor 5 (CCR5); Human Papillomavirus (HPV); Sample Adequacy Control (SAC); OncoPredict HPV Quality Control (QC) assay

Online: 5 September 2024 (00:26:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0374.v2

Detection of Meteoritic Amino Acids in Extremely Small Sample Sizes: Implications for Sample Return Missions

Christian Potiszil, Masahiro Yamanaka, Tsutomu Ota, Ryoji Tanaka, Katsura Kobayashi, Eizo Nakamura

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Carbonaceous chondrite; sample return; amino acid; ultrahigh performance liquid chromatography; Orbitrap-mass spectrometry; organic matter; astrobiology; origin of life

Online: 20 March 2023 (04:36:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0413.v1

Insect Protein as a Component of Meat Analogue Burger

Anna Krawczyk, Juana Fernández-López, Anna Zimoch-Korzycka

Subject: Biology And Life Sciences, Food Science And Technology Keywords: edible insect; Alphitobius diaperinus; alternative protein source; consumer acceptance; quality properties; texture profile

Online: 7 May 2024 (15:55:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0826.v1

Childhood Emotional Abuse, Neuroticism, Perfectionism and Workaholism in an Italian Sample of Young Workers

Valeria Verrastro, Francesca Cuzzocrea, Danilo Calaresi, Valeria Saladino

Subject: Social Sciences, Psychology Keywords: childhood emotional abuse; neuroticism; perfectionism; workaholism; gender differences; emerging adults

Online: 15 February 2024 (09:39:23 CET)

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Preprint REVIEW | doi:10.20944/preprints202409.1810.v1

Indoleamine 2,3-Dioxygenase 2 (IDO2): The Protein That Lived Twice

Chiara Suvieri, Maria Laura Belladonna, Claudia Volpi

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: indoleamine 2,3-dioxygenase 2 (IDO2); cancer; signaling function; tryptophan metabolism; indoleamine 2,3-dioxygenase 1 (IDO1); apo-enzyme

Online: 23 September 2024 (15:31:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1113.v1

The Effect of Sample Configuration on the Measurement of the Bending Properties of Extruded Polystyrene Using Three-Point, Four-Point, and Compression Bending Tests

Hiroshi Yoshihara, Masahiro Yoshinobu, Makoto Maruta

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: extruded polystyrene; three-point bending test; four-point bending test; compression bending test; Young’s modulus; proportional limit stress; bending strength

Online: 17 May 2024 (04:17:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2438.v1

Alcohol Consumption and Cancer Risk: Two Sample Mendelian Randomization

Yongho Jee, Mikyung Ryu, Jae Woong Sull

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Alcohol consumption; causality; Mendelian randomization; cancer

Online: 30 July 2024 (09:12:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1633.v1

Microcystis viridis NIES-102 Algae Lectin MVL Interacts with the COVID-19 Spike Protein RBDs via Protein-Protein Interaction

Zhengguang Wang, Zhihan Yang, Mami Shishido, Khadija Daoudi, Masafumi Hidaka, Hiroaki Tateno, Eugene Futai, Tomohisa Ogawa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Lectin; COVID-19; Algae; Carbohydrate-specificity; Protein-protein interaction

Online: 24 May 2024 (14:12:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0872.v1

Probability Distribution-Guided Adversarial Sample Attacks

Hongying Li, Miaomiao Yu, Xiaofei Li, Jun Zhang, Shuohao Li, Jun Lei, Hairong Huang

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Probability Distribution; Adversarial Sample; Transferability; Interpretability

Online: 12 May 2023 (03:13:00 CEST)

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In recent years, with the rapid development of technology, artificial intelligence(AI) security issues represented by adversarial sample attack have aroused widespread concern in society. Adversarial samples are often generated by surrogate models and then transfer to attack the target model, and most AI models in real-world scenarios belong to black boxes, thus transferability becomes a key factor to measure the quality of adversarial samples. The traditional method relies on the decision boundary of the classifier and takes the boundary crossing as the only judgment metric without considering the probability distribution of the sample itself, which results in an irregular way of adding perturbations to the adversarial sample, an unclear path of generation, and a lack of transferability and interpretability. In the probabilistic generative model, after learning the probability distribution of the samples, a random term can be added to the sampling to gradually transform the noise into a new independent and identically distributed sample. Inspired by this idea, we believe that by removing the random term, the adversarial sample generation process can be regarded as static sampling of the probabilistic generative model, which guides the adversarial samples out of the original probability distribution and into the target probability distribution, and helps to improve transferability and interpretability. Therefore, we propose a Score Matching-Based Attack(SMBA) method to perform the adversarial sample attacks by manipulating the probability distribution of the samples, which can show good transferability in the face of different datasets and models, and give reasonable explanations from the perspective of mathematical theory and feature space. In conclusion, our research establishes a bridge between probabilistic generative models and adversarial samples, provides a new entry angle for the study of adversarial samples, and brings new thinking to AI security.

Preprint ARTICLE | doi:10.20944/preprints202404.0758.v1

Positional and Dimensional Tmj Changes Following the Use of Different Class II Correctors in a Sample of Erbil City [Randomized Clinical Trial]

Rebin Mohammedameen, Omar fawzi Chawshli

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: fixed functional appliance; MRI; Class II; articular disc; Tmj

Online: 11 April 2024 (14:08:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1169.v1

Puzzling Regulation of Interferon Signaling System by p53 Tumor Suppressor Protein

Agnieszka Będzińska, Barbara Łasut-Szyszka, Małgorzata Krześniak, Agnieszka Gdowicz-Kłosok, Marek Rusin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: p53; interferon; STAT1; NK-92; caspase-1; innate immunity

Online: 17 April 2024 (14:47:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0445.v1

HIV Protein Nef Induces Cardiomyopathy through Induction of Bcl2 and p21

Olena Kondrachuck, Pierce Ciccone, Nicole Ford, Kim Hong, Yuka Kimura, Jorgo Zi, Sumaya Yusuf, Aya Alkousa, Nishit Tailor, Rithvik Rajakumar, Jay Rappaport, Manish Gupta

Subject: Biology And Life Sciences, Aging Keywords: HIV; Nef; autophagy; cardiomyopathy; Bcl2; Beclin-1; fibrosis; P21

Online: 5 September 2024 (11:00:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0199.v1

Sub-Millisecond Integrated Mix-and-Inject Microfluidic Sample Delivery Devices

Majid Hejazian, Eugeniu Balaur, Brian Abbey

Subject: Physical Sciences, Acoustics Keywords: Microfluidics; Micro-Jet; Sub-millisecond mixing; Simulation; Sample delivery for XFEL

Online: 8 February 2021 (12:12:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1268.v1

Is Water the Engine of Protein Folding?

Marco Campanile, Giuseppe Graziano

Subject: Biology And Life Sciences, Biophysics Keywords: water; globular proteins; folding; hydrophobic effect

Online: 17 September 2024 (08:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0607.v1

Affective Neuroscience and Sex Differences in the Relationship between Anger and Fear. If Fear Increases, Seeking and Play Decrease, in the Female Sample.

Paola Manfredi

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Sex differences; basic affective systems; SEEKING; FEAR; ANGER; PANIC; PLAY; CARE; gender inequality

Online: 8 August 2024 (12:05:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1849.v1

Analysis of Cow Milk Fatty Acid and Protein Composition in Different Breeds and Regions

Yunxia Zou, Yifei Chen, Qingyong Meng, Yachun Wang, Yali Zhang

Subject: Biology And Life Sciences, Other Keywords: bovine milk; breeds; fatty acids; protein composition; region

Online: 24 September 2024 (04:49:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0023.v1

An In-silico Designing of Vaccine against All Serotypes of Dengue Virus Based on Virtual Screening of B-Cell and T-Cell Epitopes

Hikmat Ullah, Shaukat Ullah, Li Jinze, Fan Yang, Lei Tan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Dengue virus; in-silico designing; B cell epitope; T cell Epitopes; pan-serotype vaccine; molecular docking

Online: 1 July 2024 (09:12:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201701.0040.v1

Sarcocystis neurona Protein Kinases: Computational Identification, Evolutionary Analysis and Putative Inhibitor Docking

Edwin Kimathi Murungi, Henry Muriuki Kariithi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sarcocystis neurona; EPM; docking; apicomplexans; phylogeny; homology modeling

Online: 9 January 2017 (05:19:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202405.0005.v1

Precision Fermentation as an Alternative to Animal Protein, a Review

Marilia M. Knychala, Larissa A. Boing, Jaciane L. Ienczak, Débora Trichez, Boris U. Stambuk

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Precision fermentation; recombinant casein; recombinant whey proteins; recombinant egg-white proteins; recombinant collagen; recombinant hemoglobin; recombinant myoglobin; recombinant albumin; cellular agriculture

Online: 1 May 2024 (07:39:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0558.v1

Long COVID: G Protein-Coupled Receptors (GPCRs) Associated Genes and Pathways as a Promising Therapeutic Potential Through Computational Analysis

Dhanyashri Guruparan, Suresh Kumar

Subject: Biology And Life Sciences, Virology Keywords: long COVID; GPCR signaling pathways; therapeutic targets; drug repurposing; bioinformatics

Online: 8 August 2024 (12:18:41 CEST)

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Long COVID, also referred to as post-COVID-19 condition, describes the lingering after-effects of COVID-19 infection. This complex syndrome encompasses a range of symptoms affecting physical well-being, cognitive function, and mental health. The widespread impact of long COVID extends beyond individual sufferers, placing significant strain on healthcare systems and causing far-reaching socioeconomic consequences. The scale of this multifaceted health crisis is unparalleled in recent history, affecting millions globally and challenging our understanding of post-viral conditions. This study aimed to explore the possibility of targeting G protein-coupled receptors (GPCRs) signaling pathway-related genes as therapeutics for long COVID using computational analysis. Targeting GPCRs and their signaling pathways holds significant promise for long COVID therapeutics, as these receptors are involved in crucial physiological processes including immune regulation, inflammation control, and neurotransmission. All of these are implicated in the diverse and persistent symptoms of long COVID, potentially offering a versatile approach to addressing multiple aspects of the condition simultaneously. This study identified 21 genes associated with GPCRs for further exploration as potential targets. Notably, GPCR genes such as HRAS, KRAS, GNAQ, and GNA11 were determined to be possible therapeutic targets for the pathophysiological mechanisms of long COVID. FDA-approved drugs targeting these genes were identified, including Binimetinib, Cabozantinib, Selumetinib, Panitumumab, Cetuximab, Adagrasib, Tipifarnib, and Sotorasib. Additionally, Naltrexone and Tipifarnib have emerged as potential new treatment options for long COVID. SARS-CoV-2 may hijack GPCR signaling pathways to dysregulate lung ion and fluid transport, contributing to the pathophysiology of pulmonary edema in patients with COVID-19. Further research is required to translate the findings into the development of therapeutics and to investigate the relationship between viral infection and GPCR signalling.

Preprint ARTICLE | doi:10.20944/preprints202408.1006.v1

The Usefulness of the Regression-Based Normed SKT Short Cognitive Performance Test in detecting Cognitive Impairment in a Community Sample

Mark Stemmler, Melina Arnold, Katya Numbers, Nicole A. Kochan, Perminder S. Sachdev, Henry Brodaty

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: cognitive assessment; regressions-based norming; dementia; MCI; sensitivity; specificity; Sydney Memory and Aging Study (MAS); short cognitive performance test (SKT)

Online: 14 August 2024 (03:42:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1062.v1

Sample Plots Forestry Parameters Verification and Updating Using Airborne LiDAR Data

Jie Wang, Chunjing Yao, Hongchao Ma, Junhao Xu, Chen Qian

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: LiDAR; Tree Segmentation; Tree Species Identification; Tree Species Identification; DBN; Forest Parameter

Online: 27 April 2023 (09:35:20 CEST)

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The rapid development of LiDAR technology has promoted great changes in forest resource surveys. The airborne LiDAR point cloud can provide precise tree height and detailed vertical structure of the tree stands. Coordinating some representative ground sample plots, LiDAR can be used to estimate key forest resource indicators such as forest stock volume, diameter at breast height, and forest biomass at a large scale. By establishing relationship models between the forest parameters of sample plots and the calculated parameters of LiDAR, these developments may eventually expand the models to large-scale forest resource surveys of entire areas. In this study, eight sample plots in northeast China are used to verify and update the information using point cloud obtained by the LiDAR scanner riegl-vq-1560i. Firstly, the tree crowns are segmented using the profile-rotating algorithm, and dominant trees height are used to check and rectify the tree locations. Secondly, considering the correlation between forestry parameters and tree species, we establish models to distinguish between species using geometric characteristics of tree crowns. Thirdly, when the tree species is known, parameters such as height, crown width, diameter at breast height, biomass and stock volume can be extracted from trees. The prediction models of forestry parameters can also be verified, which can be extended to accurate large-scale forestry surveys based on LiDAR data. Finally, experiment results demonstrate that the F-score of the eight plots in the tree segmentation exceed 0.95, the accuracy of tree species correction exceeds 90%, and the R2 of tree height, east-west canopy width, north-south canopy width, diameter at breast height, above-ground biomass and stock volume are 0.893, 0.757, 0.694, 0.840, 0.896 and 0.891, respectively. The above results indicate that the LiDAR-based estimation of forestry parameters is practical and that these forestry parameter prediction models can be widely applied in forest resource monitoring.

Preprint REVIEW | doi:10.20944/preprints202403.0120.v1

A Review for Artificial Intelligence Based Protein Subcellular Localization

Hanyu Xiao, Yijin Zou, Jieqiong Wang, Shibiao Wan

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Protein Subcellular Localization; Machine Learning; Deep Learning; Artificial Intelligence; Gene Ontology; Sequence Analysis

Online: 4 March 2024 (05:55:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0409.v1

Clinical Features of Hospitalized Stroke Patients Infected with SARS-CoV-2 Omicron Variant

Hiroshi Kanno, Kazuki Miyazaki, Sachiko Yamada, Shutaro Matsumoto, Yuki Sagehashi, Satoru Takahashi, Yongson Kim, Keiko Namiki, Satoshi Fujii

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Stroke; COVID-19; SARS-CoV-2 Omicron variant; modified Ranking scale; coagulopathy

Online: 9 October 2023 (09:30:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0908.v1

A Green Laboratory Approach to Medical Sample Transportation: Assessing the Carbon Dioxide (CO2) Footprint of Medical Sample Transportation by Drone, Combustion Car, and Electric Car

Noel Rouven Stierlin, Fabian Loertscher, Martin Risch, Lorenz Risch

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: Sustainable Healthcare Logistics; Medical Sample Transportation; CO2 Emissions; Drone Technology; Environmental Impact; Operational Efficiency

Online: 13 August 2024 (08:56:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1369.v1

Placental Protein 13 (PP13): Vasomodulatory Effects on Human Uterine Arteries and Potential Implications for Preeclampsia

Mariacarmela Gatto, Milena Esposito, Michele Morelli, Silvia De Rose, Sveinbjorn Gizurarson, Hamutal Meiri, Maurizio Mandalà

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: Galectin; Nitric-oxide; cGMP; vascular tone; Gestational hypertension

Online: 20 June 2024 (00:11:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1000.v1

Comparative Evaluation of Lipid Profile, C-reactive Protein and Paraoxonase-1 Activity in Dogs with Inflammatory Protein-Losing Enteropathy and Healthy Dogs

Paola Gianella, Federica Cagnasso, Alessia Giordano, Antonio Borrelli, Enrico Bottero, Barbara Bruno, Riccardo Ferriani, Franca Borella, Sara Meazzi, Donatella Scavone, Saverio Paltrinieri

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: canine; chronic enteropathy; cholesterol; CRP; IBD; lipoprotein; PLE; PON-1; triglycerides

Online: 13 September 2024 (03:02:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0824.v1

Emotional Knowledge in a Sample of University Students

Marta Sainz-Gómez, Rosario Bermejo, María José Ruiz-Melero

Subject: Social Sciences, Education Keywords: Emotional intelligence; scale; higher education; emotional knowledge; teacher training

Online: 13 December 2023 (04:56:00 CET)

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One of the current challenges in Higher Education is the development of students’ emotional competencies, and consequently, how these competencies can be trained. Moreover, this must be addressed within the UN framework of inclusive, equitable, quality education (United Nations Agenda 2030) (SDG 4). To address this challenge, the present study set the following objectives: (1) to analyze pre-service teachers’ level of interest in EI and their perceived level of general EI knowledge, (2) to examine their self-perception of certain variables of emotional knowledge, according to their degree program and year of studies, (3) to analyze the relationship between these emotional knowledge variables and perceived emotional intelligence (EI); and (4) to study the predictive capacity of the study variables on future teachers' emotional knowledge variables. The study sample contained a total of 434 university students from three degree programs at two universities in Spain, of which 82.70% were women, and the mean age was 20.91 years. Two instruments were used: an ad hoc questionnaire, which assessed perceived emotional knowledge according to three variables (General knowledge of EI, where level of training in EI is considered; Intrapersonal knowledge of EI; and Theoretical-practical knowledge related to teaching), and the Trait Meta-Mood Scale (TMMS-24), which assesses metaknowledge of emotional states across three variables (Attention, Clarity, and Repair). Results of the study suggest that there are differences in perceived EI knowledge according to students' year of studies and according to their degree, and indicate that students present high interest, while their perceived level of knowledge is low. In addition, emotional clarity is the EI variable that was identified as being important in the emotional knowledge of future teachers. This study shows evidence of the importance of social-emotional competencies in initial teacher training.

Preprint ARTICLE | doi:10.20944/preprints202101.0047.v1

Accelerating Gut Microbiome Research with Robust Sample Collection

Zoe J. Zreloff, Danielle Lange, Suzanne D. Vernon, Martha R. Carlin, Raul de Jesus Cano

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Stool; Fecal; Microbiome; Microbiota; Heterogeneous; Homogeneous; Sample

Online: 4 January 2021 (13:29:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0376.v1

Rock Sample Surface Preparation Influences Thermal Infrared Spectra

Evelien Rost, Christoph Hecker, Martin C. Schodlok, Freek D. van der Meer

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: thermal infrared; reflectance spectroscopy; emissivity; surface roughness; geological sample preparation; hyperspectral; drill core scanning

Online: 17 October 2018 (07:51:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201710.0191.v1

Effelsberg Monitoring of a Sample of RadioAstron Blazars

Jun Liu, Thomas P. Krichbaum, Xiang Liu, Alex Kraus, Hayley Bignall, Yuri Y. Kovalev, Kirill V. Sokolovsky, J. Anton Zensus

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: galaxies; active -- method; statistical -- radio continuum; ISM

Online: 31 October 2017 (04:18:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0594.v1

Compensatory Modulation of Seed Storage Protein Synthesis and Starch Accumulation by Selective Editing of 13 kDa Prolamin Genes in Rice

Hue Anh Pham, Kyoungwon Cho, Anh Duc Tran, Deepanwita Chandra, Jinpyo So, Hanh Thi Thuy Nguyen, Hyunkyu Sang, Jong-Yeol Lee, Oksoo Han

Subject: Biology And Life Sciences, Plant Sciences Keywords: prolamin; 13 kDa prolamin; glutelin; seed storage protein; CRISPR-Cas9; protein body; starch; rice seed; ER stress; differentially expressed genes

Online: 9 May 2024 (11:58:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0672.v1

Characterization Among and within Sicilian Tetraploid Wheat Landraces by Grain Storage Protein Analysis

Samuela Palombieri, Marco Bonarrigo, Silvia Potestio, Francesco Sestili, Bernardo Messina, Giuseppe Russo, Claudia Miceli, Benedetto Frangipane, Marco Genduso, Chiara Delogu, Lorella Andreani, Stefania Masci

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: durum wheat; landraces; traceability; gliadin; glutein subunits

Online: 12 February 2024 (13:15:39 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.0150.v1

Heat Shock Protein 60 as an Immunomodulator in Heart Diseases

Vahid Saqagandomabadi, Adelaide Carista, Valentina Di Felice

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Heart; diseases; heat shock proteins; Hsp60; cardiac cells

Online: 3 August 2024 (16:01:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1597.v1

Bidirectional Two-Sample Mendelian Randomization Analysis Identifies Causal Associations between Gut Microbiome and Sleep-Related Traits

Mingxia Zhai, Weichen Song, Zhe Liu, Wenxiang Cai, Guanning Lin

Subject: Biology And Life Sciences, Other Keywords: Gut Microbiome; Bacterial Pathways; Sleep-related Traits; Mendelian Randomization; Causal Relationship

Online: 28 February 2024 (10:53:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1219.v1

Roles of Mature Domain Targeting Signals for Protein Translocation and Secretion in Lactococcus lactis

Mai Ngoc Hoang, Clemens Peterbauer

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Lactococcus lactis; secretory proteins; signal peptide; mature domains; hydrophobic patches; SecA

Online: 16 August 2024 (08:28:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0679.v1

Pycnogenol-Assisted Alleviation of Titanium Dioxide Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation

Je-Oh Lim, Woong-Il Kim, So-Won Pak, Se-Jin Lee, Changjong Moon, In-Sik Shin, Sung-Hwan Kim, Jong-Choon Kim

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Titanium dioxide nanoparticle; lung inflammation; pycnogenol; antioxidative effect; thioredoxin-interacting protein

Online: 9 July 2024 (08:20:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0001.v1

On Near Optimality of One-sample Update for Joint Detection and Estimation

Yang Cao, Liyan Xie, Yao Xie, Huan Xu

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: sequential methods; change-point detection; online algorithms

Online: 1 September 2017 (05:24:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0298.v1

A TriAdj-Adjuvanted Chlamydia trachomatis CPAF Protein Vaccine Is Highly Immunogenic in Pigs.

Jessica A Proctor, Maria Stadler, Lizette M Cortes, David Brodsky, Lydia Poisson, Volker Gerdts, Alex I Smirnov, Tatyana I Smirnova, Subarna Barua, Kenneth W Beagley, Jon M Harris, Toni Darville, Tobias Käser

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Chlamydia trachomatis; vaccination; adjuvant; swine; One Health; animal model; chlamydial protease-like activity factor

Online: 6 March 2024 (10:48:38 CET)

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Chlamydia trachomatis (Ct) infections are the most common sexually-transmitted infection (STI). Despite effective antibiotics for Ct, undetected infections or delayed treatment can lead to infertility, ectopic pregnancies, and chronic pelvic pain. Besides humans, chlamydia poses similar health challenges in animals such as C. suis (Cs) in pigs. Based on the similarities between humans and pigs as well as their chlamydia species, we use pigs as a large biomedical animal model for chlamydia research. In this study, we use the pig model to develop a vaccine candidate against Ct. The vaccine candidate consists of TriAdj-adjuvanted chlamydial protease-like activity factor (CPAF) protein. We tested two weekly administration options – twice intranasal (IN) followed by twice intramuscular (IM), or twice IM followed by twice IN. We assessed the humoral immune response not only in serum by CPAF-specific IgG including antibody avidity determination but also in cervical and rectal swabs by CPAF-specific IgG and IgA ELISAs. The systemic T-cell response was analyzed upon in vitro CPAF-restimulation via IFN-γ and IL-17 ELISpots as well as intracellular cytokine staining flow cytometry. Our data demonstrate that while the IN/IM vaccination mainly led to non-significant systemic immune responses, the vaccine candidate is highly immunogenic if administered IM/IN. This vaccination strategy induced high serum anti-CPAF IgG levels with strong avidity as well as high IgA and IgG levels in vaginal and rectal swabs and in uterine horn flushes. In addition, this vaccination strategy prompted a pronounced cellular immune response: Besides inducing IL-17 production, the vaccine candidate induced a strong IFN-γ response by CD4 T cells. In the IM/IN vaccinated pigs, these cells also significantly downregulated their CCR7 expression – a sign for differentiation into peripheral tissue homing effector/ memory cells. Conclusively, this study demonstrates strong immunogenicity of the IM/IN administered TriAdj-adjuvanted Ct CPAF vaccine candidate. Future studies will test vaccine efficacy of this promising Ct vaccine candidate. In addition, this project demonstrates suitability of the Cs pre-exposed outbred pig model for Ct vaccine development. Thereby, we aim to open the bottleneck of large animal models to facilitate the progression of Ct vaccine candidates into clinical trials.

Preprint ARTICLE | doi:10.20944/preprints202403.1254.v1

Soybean Meal Protein Extraction and Comparative Analysis with Soy Protein Isolate

Kudirat Alarape, Adewale Adeniyi, Tawakalt Ayodele, Ibrahim Bello, Niloy Chandra Sarker, Ademola Hammed

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Soybean meal; Protein isolates; Extraction; Enzymatic hydrolysis; Characterization

Online: 21 March 2024 (08:20:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1229.v1

Role of Protein Tyrosine Phosphatase Receptor Type E (PTPRE) in Chemoresistant Retinoblastoma

Lars Mohren, Annika Doege, Natalia Miroschnikov, Oliver Dräger, Maike Anna Busch, Nicole Dünker

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: PTPRE; miR631; retinoblastoma; CAM assay; etoposide; chemoresistance; tumorigenesis

Online: 20 March 2024 (13:13:44 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.1194.v1

Current Advancements in Drone Technology for Medical Sample Transportation

Noel Rouven Stierlin, Lorenz Risch, Martin Risch

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: drone technology; medical logistics; healthcare delivery; UAVs; medical sample transportation; healthcare innovation; AI-driven navigation; rural healthcare

Online: 16 August 2024 (09:56:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0661.v1

Extreme Behavior of Competing Risks with Random Sample Size

Long Bai, Kaihao Hu, Conghua Wen, Zhongquan Tan, Chengxiu Ling

Subject: Business, Economics And Management, Finance Keywords: extreme value theory; competing risks; random sample size

Online: 9 July 2024 (10:21:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0412.v1

Candida Massiliensis sp. nov. Isolated from A Clinical Sample

Jihane Kabtani, Fatima BOULANOUAR, Muriel Militiello, Carole Cassagne, Stéphane Ranque

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: biolog phenotypic technology; Candida; energy-dispersive X-ray spectroscopy; genotype; multilocus DNA sequencing; one new taxon; yeast

Online: 26 October 2022 (10:15:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0329.v1

Are Viruses Taxonomic Units? – A Protein Domain and Loop-Centric Phylogenomic Assessment

Gustavo Caetano-Anollés

Subject: Biology And Life Sciences, Virology Keywords: holobionts; hologenomes; LUCA; origin of viruses; phylogenomic reconstruction; process; protein structure; reductive evolution; superkingdom; syncytins

Online: 6 June 2024 (07:52:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1704.v1

Vision System for the Mars Sample Return Capture, Containment and Return System (CCRS)

Brent Jon Bos, David Donovan, John Capone, Chen Wang, Terra Hardwick, Dylan Bell, Yuqing Zhu, Robert Podgurski, Bashar Rizk, Ireneusz Orlowski, Rachel Edison, David Harvey, Brianna Dizzon, Lindsay Haseltine, Kristoffer Olsen, Chad Sheng, Robert Bousquet, Luan Vo, Georgi Georgiev, Kristen Washington, Michael Singer, Stefan Ioana, Anloc Le, Elena Georgieva, Michael Hackett, Michael Ravine, Michael Caplinger, Phillip Coulter, Erin Percy, Charles Torisky, Jean Lauenstein, Kaitlyn Ryder, Michael Campola, Dillon Johnstone, William J. Thomes, Richard G. Schnurr, John McCloskey, Eugenia DeMarco, Ellen Lee, Calinda Yew, Bo Yang, Mingyu Han, Bartosz Blonski

Subject: Engineering, Aerospace Engineering Keywords: Mars; cameras; sample return; space optics

Online: 26 April 2024 (09:33:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1057.v1

Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 in C. reinhardtii

Laura Mosebach, Shin-Ichiro Ozawa, Muhammad Younas, Huidan Xue, Martin Scholz, Yuichiro Takahashi, MIchael Hippler

Subject: Biology And Life Sciences, Plant Sciences Keywords: photosystem I; chemical crosslinking; mass spectrometry; Chlamydomonas reinhardtii

Online: 16 April 2024 (15:57:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0053.v2

Machine Learning Driven Dashboard for Chronic Myeloid Leukemia Prediction using Protein Sequences

Waqar Ahmad, Abdul Raheem Shahzad, Muhammad Awais Amin, Waqas Haider Bangyal, Tahani Jaser Alahmadi, Saddam Hussain Khan

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Protein Sequences; Pseudo-AAC; AAC; Dipeptide-C; Machine Learning Classifiers; Chronic Myeloid Leukemia; Blood Cancer

Online: 26 June 2024 (10:02:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1609.v1

Effect of Multi-mode Divergent Ultrasound Pretreatment on Hardness, Microstructure and Digestion of Acid-Induced Whey Protein Gels

Yu Cheng, Xiaolong Shi, Georgina Benewaa Yeboah, Lihong Chen, Juan Wu

Subject: Biology And Life Sciences, Food Science And Technology Keywords: ultrasound; frequency; whey protein; gel; acid induced; digestion

Online: 24 May 2024 (08:38:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0065.v1

Bayesian Structural Equation Modeling: Goodness of Fit and Estimation Precision across Sample Sizes

Diana Mindrila

Subject: Social Sciences, Education Keywords: structural equation modeling; Bayesian analysis; maximum likelihood

Online: 1 May 2024 (08:21:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1073.v1

A Two-Sample Mendelian Randomization Analysis Investigates Associations Between Gut Microbiota and Dyslipidemia

Xuyi Zhou, Peiqi Lian, Hui Liu, Yinghui Wang, Meijuan Zhou, Zhijun Feng

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: dyslipidemia; gut microbiota; mendelian randomization

Online: 18 September 2023 (05:54:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0312.v1

Rapid Assessment of Lipidomics Sample Purity and Quantity Using Fourier-Transform Infrared Spectroscopy

Harley Robinson, Jeffrey Molendijk, Alok K. Shah, Tony Rahman, Gregory J. Anderson, Michelle M. Hill

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: lipids; phospholipids; sphingolipids; triglycerides; FTIR; mass spectrometry; chemical contaminants

Online: 21 July 2022 (08:03:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0717.v1

Potential Inhibitors of Lumpy Skin Disease’s Viral Protein (DNA Polymerase): A Combination of Bioinformatics Approaches

Sabbir Zia, Md-Mehedi Sumon, Md-Ashikur Ashik, Abul Basar, Sangjin Lim, Yeonsu Oh, Yungchul Park, Md Mafizur Rahman

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Lumpy skin disease; DNA polymerase; potential inhibitors; phytocompounds; antiviral drug compounds; molecular docking

Online: 13 March 2024 (07:58:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.0459.v1

Tanapox Virus and Yaba Monkey Tumor Virus K3 Orthologs Inhibit Primate Protein Kinase R in a Species-Specific Fashion

Dewi Megawati, Jeannine N. Stroup, Chorong Park, Taylor Clarkson, Loubna Tazi, Greg Brennan, Stefan Rothenburg

Subject: Biology And Life Sciences, Virology Keywords: Protein kinase R, poxviruses, K3L, yatapoxvirus, translational regulation

Online: 7 June 2024 (08:08:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1415.v1

The Association between Dietary Protein Intake and Sources and the Rate of Longitudinal Changes in Brain Structure

Fusheng Cui, Huihui Li, Yi Cao, Weijing Wang, Dongfeng Zhang

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: dietary protein; animal protein; vegetable protein; sources of protein; brain structure markers; UK Biobank

Online: 25 March 2024 (13:07:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.1653.v2

The p53 tumor suppressor protein activates transcription of DUSP13 isoform from the alternative promoter in intron

Małgorzata Krześniak, Barbara Łasut-Szyszka, Agnieszka Będzińska, Agnieszka Gdowicz-Kłosok, Marek Rusin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: p53; MDM2; DUSP13; alternative promoter; dual-specificity phosphatase

Online: 26 February 2024 (16:55:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0838.v1

Reference Values for the German Version of the Quality of Life after Brain Injury in Children and Adolescents (QOLIBRI-KID/ADO) from a General Population Sample

Leonie Krol, York Hagmayer, Nicole von Steinbuechel, Katrin Cunitz, Anna Buchheim, Inga K. Koerte, Marina Zeldovich

Subject: Social Sciences, Psychology Keywords: reference values; general population; patient-reported outcome measure (PROM); health-related quality of life; children and adolescents; traumatic brain injury

Online: 15 February 2024 (10:02:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.1691.v1

High-Biotin Sample Interference on Antibodies Concentration by Sandwich Immunoassays

Geraldo Balieiro Neto, Jair Rodini Engracia Filho, Fabio Enrique Lemos Budino, Acyr Wanderley de Paula Freitas, Weber Vilas Boas Soares

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: antibody-antigen complex; biotin; ELISA; immunoassays; interference; streptavidin

Online: 24 August 2023 (09:53:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0528.v1

Sample Delivery Systems for Serial Femtosecond Crystallography at PAL-XFEL

Jaehyun Park, Ki Hyun Nam

Subject: Biology And Life Sciences, Biophysics Keywords: serial femtosecond crystallography; X-ray free electron laser; sample delivery; injector; fixed-target scanning; hybrid method

Online: 19 April 2023 (03:22:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202010.0273.v1

Multistage Sample Average Approximation for Harvest Scheduling under Climate Uncertainty

Martin Bagaram, Sándor Tóth

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: data-driven optimization; climate change; harvest planning; optimality gap; forest

Online: 13 October 2020 (10:46:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201911.0388.v1

Social Noise Exposure in the Sample of Slovak University Students

Alexandra Filova, Jana Jurkovicova, Katarina Hirosova, Diana Vondrova, Barbora Filova, Martin Samohyl, Jana Babjakova, Juraj Stofko, Lubica Argalasova

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: social noise; auditory, non-auditory noise effects; personal music players; university students

Online: 30 November 2019 (10:07:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0081.v1

Multi-Elemental Analysis of Hair and Fingernails by ED-XRF without Sample Grinding and Mineralization

Zofia Mierzyńska, Maria Niemirska, Kacper Zgonina, Tomasz Bieńkowski, Krzysztof Hryniów, Paweł Świder, Katarzyna Pawlak

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: metal determination; hair; nails; pellets formation; solubilization of keratin; ED-XRF; ICP-MS

Online: 3 January 2024 (02:11:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0213.v1

Microbial Protein Production using Lignocellulosic Biomass and Nitrogen Fixation

Tawakalt Ayodele, Kudirat Alarape, Ibrahim Adebayo Bello, Abodunrin Tijani, Liadi Musiliu, Ademola Hammed

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: Microbial protein; Lignocellulosic biomass; switchgrass hydrolysate; Klebsiella oxytoca M5A1; Fermentation, Organic acids.

Online: 2 April 2024 (15:41:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2342.v1

Comparative Study on the Effect of Calcium Chloride on the Physicochemical Properties of Glycerol- and Sorbi-tol-Plasticized Pea Protein Isolate Films

Dariusz Kowalczyk

Subject: Chemistry And Materials Science, Biomaterials Keywords: edible films; pea protein; water vapor permeability; opacity; tensile strength

Online: 29 July 2024 (16:27:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0806.v1

BiFC Magic: Shedding Light on Protein-Protein Interactions

Yunlong JIA, Françoise Bleicher, Samir Merabet

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: BiFC; PPIs

Online: 12 October 2023 (20:17:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0405.v1

Small Sample Hyperspectral Image Classiﬁcation Based on the Random Patches Network and Recursive Filtering

Denis Uchaev, Dmitry Uchaev

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: hyperspectral data; few-shot learning; deep features; convolution kernels; edge-preserving filtering

Online: 22 December 2022 (01:44:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202101.0298.v1

Mindfulness in Sexual Activity, Sexual Satisfaction and Erotic Fantasies in a Non-Clinical Sample

Laura C. Sánchez-Sánchez, María Fernanda Valderrama Rodríguez, José Manuel García-Montes, Cristina Petisco-Rodríguez, Rubén Fernández-García

Subject: Social Sciences, Psychology Keywords: mindfulness; meditation; sexual satisfaction; sexual activity; sexual/erotic fantasies

Online: 15 January 2021 (14:15:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202003.0388.v1

A Two-Sample Mendelian Randomization Analysis Investigates Associations between Gut Microbiota and Celiac Disease

Iraia García-Santisteban, Ariadna Cilleros-Portet, Elisabet Moyua-Ormazabal, Alexander Kurilshikov, Alexandra Zhernakova, Koldo Garcia-Etxebarria, Nora Fernandez-Jimenez, Jose Ramon Bilbao

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: celiac disease; gut microbiota; mendelian randomization

Online: 26 March 2020 (14:08:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202002.0187.v1

Sample Preservation and Plant Sex Prediction in White Guinea Yam (Dioscorea rotundata Poir.) Seedlings

Asrat Asfaw, Paterne Agre, Chidinma Nwachukwu, Bunmi Olasanmi, Jude Obidiegwu, Emmanuel Nwachukwu, Patrick Adebola, David De Koeyer

Subject: Biology And Life Sciences, Plant Sciences Keywords: dioecious; DNA quality; flower type; sample preservation method; sex genotype; sex phenotype; visual assay

Online: 14 February 2020 (04:22:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202003.0010.v1

Casein Protein Hydrolysate Fed Human Serum From Older Adults Regulates In Vitro Muscle Protein Synthesis, Muscle Protein Breakdown and Myotube Growth

Martina Pauk, Miryam Amigo-Benavent, Bijal Patel, Philip M. Jakeman, Brian P. Carson

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: skeletal muscle; muscle protein synthesis; muscle protein breakdown; serum; hydrolysate

Online: 1 March 2020 (11:52:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0836.v1

Effect of Protein (BSA) Content on the Frictional Behaviour of Soft Contact Lenses Using a Dynamic Oscillating Tribometer

Luís Vilhena, Amílcar Ramalho

Subject: Engineering, Bioengineering Keywords: biotribology; friction; soft contact lenses; protein content

Online: 12 April 2024 (05:31:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0589.v1

Synthetic Image Generation using Conditional GAN Provided Single Sample Face Image

IQBAL MUHAMMAD ALI, Waqas Jadoon, Soo Kyun Kim

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Convolutional Neural Network (CNN); Single Sample Per Person (SSPP); Conditional Generative Adversarial Network (CGAN)

Online: 8 April 2024 (16:59:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202012.0237.v1

Multi-Block Color-Binarized Statistical Images for Single Sample Face Recognition

Insaf Adjabi, Amir Benzaoui, Abdeldjalil Ouahabi, Sebastien Jacques

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: Biometrics; Face Recognition; Single Sample Face Recognition; Binarized Statistical Image Features; K-Nearest Neighbors

Online: 9 December 2020 (18:25:02 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202008.0030.v1

Household Representative Sample Strategy for COVID-19 Large-Scale Population Screening

John Takyi-Williams

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; group testing; household; screening

Online: 2 August 2020 (12:48:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1890.v1

Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression

Sakura Hirokane, Tomohiro Kawasumi, Sachio Takeno, Yukako Okamoto, Seita Miyamoto, Rikuto Fujita, Chie Ishikawa, Takashi Oda, Yuichiro Horibe, Takashi Ishino, Takao Hamamoto, Tsutomu Ueda

Subject: Medicine And Pharmacology, Otolaryngology Keywords: paranasal sinus; chronic rhinosinusitis; CRS; nasal polyps; ciliated cell; mucociliary clearance; planar cell polarity; CPLANE complex proteins; WDPCP; FUZ

Online: 24 July 2024 (08:02:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2334.v1

Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes

Hiroshi Yamada, Hirona Osaka, Nanami Tatsumi, Miu Araki, Tadashi Abe, Keiko Kaihara, Ken Takahashi, Eizo Takashima, Takayuki Uchihashi, Keiji Naruse, Kohji Takei

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SYNPO2L; actinin; actin; sarcomere; cardiomyocyte

Online: 29 July 2024 (16:21:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202404.1453.v2

The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines

Donia Hanafi, Rob U Onyenwoke, K Sean Kimbro

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: GPER receptor; Therapeutic; Fallopian Tubes; Ovarian Cancer

Online: 24 April 2024 (14:11:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0642.v1

Importance of Energy, Dietary Protein Sources and Amino Acids Composition in the Regulation of Metabolism: An Indissoluble Dynamic Combination for Life

Giovanni Corsetti, Evasio Pasini, Tiziano M Scarabelli, Claudia Romano, Arashpreet Singh, Carol C Scarabelli, Francesco S Dioguardi

Subject: Biology And Life Sciences, Other Keywords: Energy; entropy; protein synthesis; amino acids; autophagy; AMPK; mTOR

Online: 11 June 2024 (08:31:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1031.v1

Autonomous Planetary Liquid Sampler (APLS) for In-Situ Sample Acquisition and Handling from Liquid Environments

Miracle Israel Nazarious, Leonie Becker, Maria-Paz Zorzano, Javier Martin-Torres

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: liquid environment; instrumentation; sample acquisition; autonomy; reusability

Online: 14 August 2024 (11:29:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1788.v1

Development of Aptamers for RNase Inactivation in Xtract-Free™ Sample Collection and Transport Medium

Luke Thomas Daum, John David Rodriguez, James P Chambers

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Xtract-Fee™ medium; sample collection; qPCR; diagnostics; extraction-free detection; NAT; point of care; home-collect; self-collect; DNA aptamers; RNase; nucleases; 3rd generation specimen collection; AptaGuard

Online: 26 April 2024 (19:59:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1111.v1

Simple, Fast and Convenient Magnetic Bead-based Sample Preparation for Detecting Viruses via Raman-Spectroscopy

Susanne Pahlow, Marie Richard-Lacroix, Franziska Hornung, Nilay Koese-Vogel, Thomas G. Mayerhoefer, Julian Hniopek, Oleg Ryabchykov, Thomas Bocklitz, Karina Weber, Ralf Ehricht, Bettina Löffler, Stefanie Deinhardt-Emmer, Juergen Popp

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: viruses; SARS-CoV-2; Raman spectroscopy; magnetic beads; sample preparation

Online: 28 April 2023 (04:46:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201812.0202.v1

Fish Protein and Lipid Interactions on the Digestibility and Bioavailability of Starch and Protein from Durum Wheat Pasta

Ajay Desai, Margaret Brennan, Xinbo Guo, Xin-An Zeng, Charles Brennan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pasta; salmon powder; glycaemic index; protein digestibility; polyphenols; antioxidant activity; bioaccessibility

Online: 17 December 2018 (15:59:54 CET)

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Working Paper REVIEW

Visualizing Protein Structures - Tools and Trends

Xavier Martinez, Matthieu Chavent, Marc Baaden

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: molecular graphics; protein visualization; software tools; virtual reality

Online: 12 January 2020 (16:26:54 CET)

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