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Preprint ARTICLE | doi:10.20944/preprints202401.1468.v1

Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR

Claudio A. Henriquez, Natalia Riquelme, Alejandra Larach, Ximena Besoain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Virus-viroid detection; Mixed infections; Comparison of diagnostic methods; CTV; HSVd; Bioamplification

Online: 19 January 2024 (07:52:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0808.v1

Quasi-solid Composite Electrolytes of Poly-(Dioxolane) & Garnet LLZO Nanofibers Enable High Energy Efficiency Lithium-Metal Batteries

Zhengqiang Li, David R. Salem

Subject: Chemistry And Materials Science, Electrochemistry Keywords: Gel-electrolytes, Lithium-metal battery, Lithium dendrites, c-LLZO nanofiber

Online: 11 April 2024 (11:48:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0991.v1

Potential Performance of Two New RT-PCR and RT-q PCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes Allowing Efficient Entomological Surveillance of Both Vector-Borne Diseases

Michel Kiréopori Gomgnimbou, Louis robert wendyam BELEM, Etienne Bilgo, Miriam Félicité Amara, Zouera Laouali, Ali Ouari, Toussaint Bayala, Kobo Gnada, Raymond Kharlis Yao, Moussa Namountougou, Ibrahim Sangaré

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: multiplex rt-pcr; multiplex rt-qpcr; molecular detection; dengue virus; chikungunya virus; mosquitoes; Burkina Faso

Online: 12 July 2024 (08:43:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint ARTICLE | doi:10.20944/preprints201901.0212.v1

One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3

Xueming Chen, Yuhuan Chen, Chunguo Liu, Xiaojun Li, Hongyu Liu, Xiaofei Bai, Ming Liu, Yun Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DHAV-1; DHAV-3; Phylogenetic analysis; One-tube RT-PCR; Simultaneously

Online: 22 January 2019 (11:08:19 CET)

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Working Paper BRIEF REPORT

Positive Rate Is Useful to Evaluate the Sufficiency of RT-PCR Test Availability for COVID-19

Sadao Suzuki

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; RT-PCR test; pre-test probability; positive rate

Online: 5 May 2020 (06:04:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1094.v1

Development and Evaluation of an In-House Real-Time Rt-Pcr Targeting nsp10 Gene for SARS-CoV-2 Detection

Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; nsp10; real-time RT-PCR; diagnostic

Online: 20 February 2024 (06:07:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints201702.0011.v1

Simulation Analysis of Solution Transportation Absorption Chiller with the Capacity from 25 RT to 1000 RT

Koji Enoki, Fumi Watanabe, Seigo Tanaka, Atsushi Akiwawa, Toshitaka Takei

Subject: Engineering, Energy And Fuel Technology Keywords: absorption chiller; thermal energy transportation; solution transportation; ammonia-water; COP; simulation

Online: 4 February 2017 (07:49:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1366.v1

Cycle Threshold Values of SARS-CoV-2 RT-PCR During Outbreaks in Nursing Homes: A Retrospective Cohort Study.

Juan Carlos Gasco-Laborda, Maria Gil-Fortuño, Maria Dolores Tirado-Balaguer, Noemi Meseguer-Ferrer, Oihana Sabalza-Baztán, Óscar Pérez-Olaso, Iris Gómez-Alfaro, Sandrine Poujois-Gisbert, Noelia Hernández-Pérez, LLedó Lluch-Bacas, Viorica Rusen, Alberto Arnedo-Pena, Juan Bautista Bellido-Blasco

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: SARS-CoV-2; PCR; cycle threshold; infections; nursing homes; outbreaks; retrospective cohort

Online: 18 September 2024 (11:47:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0381.v1

Building-Level Wastewater Monitoring for COVID-19 Using Tampon Swabs and RT-LAMP for Rapid SARS-Cov-2 RNA Detection

Aaron Bivins, Megan Lott, Marlee Shaffer, Zhenyu Wu, Devin North, Erin Lipp, Kyle Bibby

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: SARS-CoV-2; wastewater monitoring; environmental surveillance; RT-LAMP; building-level; near-source; passive sampling

Online: 17 May 2021 (10:07:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0463.v1

Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia

Katherine Escorcia-Lindo, Leidy Hurtado-Gómez, Juan Sebastian Rosero, Nataly Solano Llanos, Camilo Barrios Sánchez, Anderson Diaz Pérez, Yirys Díaz-Olmos, Jennifer García, Yesit Bello-Lemus, Leonardo C Pacheco-Londoño, Antonio J. Acosta-Hoyos, Lisandro A Pacheco-Lugo

Subject: Biology And Life Sciences, Life Sciences Keywords: RT-LAMP; Dengue virus (DENV); Sensitivity; Point-of-Care

Online: 5 September 2024 (12:37:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1390.v1

Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

Mirko Sonntag, Vanessa K. Elgeti, Yevhen Vainshtein, Lucca Jenner, Jan Mueller, Thorsten Brenner, Sebastian O. Decker, Kai Sohn

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sepsis; antimicrobial resistances; cell-free DNA; precision diagnostics; Next-Generation sequencing; suppression PCR; real-time diagnostics; nanopore sequencing

Online: 22 April 2024 (10:13:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1127.v1

Machine Learning Application to Brain Diseases Detection

Aleksandar Simović, Maja Lutovac-Banduka, Kristina Davidović, Snežana Lekić

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; application; classification; brain diseases; detection

Online: 17 November 2023 (15:32:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0439.v1

SARS-CoV-2 Detection in Fecal Samples in Sym-asymptotic Patients with Typical Findings of COVID-19 on Ag-RDT and SARS-CoV-2 RT-PCR Tests

Richard Odongo Magwanga, Benson Onyango, Alfred Ochieng, Shehu Shagari Awandu, Pamela Were, Joy Nyangasi Kirungu, Marceline Adhiambo Oloo, Erick Muok, Stephen Munga, Fredrick Ogutu, Angeline Orwa, Fredrick Okumu, Clayton Onyango, Dorothy Awuor, Francis Orata, Justine Ochieng’-Midega, Bonface Oure, Regina Nyunja, Fredrick Ade, Sharley Indechi, Erick Okuto, Benson Estambale

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: COVID19; SARS-CoV-2 virus; Oro-nasopharyngeal; fecal; vaccination; asym-symptomatic; rapid diagnostic test

Online: 28 September 2022 (11:51:21 CEST)

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Coronavirus is a disease caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged as a global pandemic in 2019 from Wuhan, China. Since its emergence, it has caused immense suffering to human life, 6.27 million lives have been lost, movement curtailed and social dynamics disrupted. The golden standard for getting samples for SARS-CoV-2 detection is through oral- nasopharyngeal swab, this method of sample collection is invasive and uncomfortable, thus stigmatized the general population, and thereby impeded the progress of controlling the spread through mass testing. Being a contact disease, mechanisms to encourage mass testing is key to reduce the spread. This study thus developed a complimentary sample type to test for SARS-CoV-2, the use of human feces. Fecal samples were collected from 100 asym-symptomatic individuals suspected to be infected with COVID-19, virus RNA was then extracted and profiled through Real Time Polymerase Chain Reaction (RT-qPCR). The antigen rapid diagnostic test revealed high positivity rate of 44%, but the real time polymerase chain reaction results on nasopharyngeal and fecal samples revealed a significant variation, high number of the patients tested positive with stool samples compared to the nasopharyngeal swabs, with 43 and 37%, respectively. SARS-CoV-2 virus was detected in both symptomatic and asymptomatic individuals; however, the symptomatic registered a higher positivity of 25% compared to 20% among the asymptomatic patients. Vaccination only lowered the risk of infection, fully and partially vaccinated lowered the infection level to 10% compared to 20% among the unvaccinated. Finally, gender parity in relation to COVID19 was evaluated, more females (56%) compared to males were recruited in this study, out of which (20; 43.4%) were positive, and 26 (56.6%) were negative based on fecal RT-qPCR outcomes. Based on the outcome of this study, rapid diagnostic test (Ag-RDT) however cheap and or fast does not provide accurate information, moreover, the virus does not stay longer within the Oro-nasopharyngeal region, thus the invalid or negative results, thus use of feces should be adopted as a confirmatory test to ascertain the COVID19 status of an individual.

Preprint ARTICLE | doi:10.20944/preprints202308.0387.v1

Acquisition of Humoral Immune Responses in Convalescent Japanese People with SARS-CoV-2 (COVID-19) Infection in 2021

Koshiro Monzen, Takanori Watanabe, Toshihiro Okabe, Hisakuni Sekino, Hironori Nakagami, Ryuichi Morishita

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2; Convalescent serum; Neutralizing antibody

Online: 4 August 2023 (12:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0446.v1

Symbolic Analysis of Classical Neural Networks for Deep Learning

Maja Lutovac-Banduka, Igor Franc, Vladimir Milićević, Nemanja Zdravković, Nikola Dimitrijević

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: machine learning; visual programming language; closed-form expression; theoretical justification; feature extraction; complexity; optimization and validation

Online: 7 November 2023 (11:25:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints201610.0071.v1

A Rapid Magnetofluidic Micromixer Using Diluted Ferrofluid

Majid Hejazian, Nam-Trung Nguyen

Subject: Engineering, Mechanical Engineering Keywords: magnetic; microfluidics; mixing; magnetoconvection; ferrofluid

Online: 18 October 2016 (08:00:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0030.v1

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi, Hussein Alburkat, Taisir Abdulelah Kadhim, Ammar M S Al-Helli, Mustafa Hamza Ghazi, Yasir Abdulameer N. Aldabbagh, Reem Numaan Talib, Sama Hussein Ali, Noor Hameed Imran, Ruaa Almohsen, Turki Muftin Saad, Pallavi Mandave

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: PCR; Salmonella Typhi; Widal Test; BHI

Online: 1 November 2022 (12:43:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1361.v2

Assessment of DNA/RNA Defend Pro: An Inactivating Sample Collection Buffer for Enhanced Stability, Extraction-Free PCR, and Rapid Antigen Testing of Nasopharyngeal Swab Samples

Mikhail Claeys, Saif Al Obaidi, Karen Bruyland, Ilse Vandecandelaere, Jo Vandesompele

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: nucleic acid amplification; rapid antigen testing; SARS-CoV-2; influenza; RSV; inactivating buffer; direct PCR; extraction-free PCR; nasopharyngeal

Online: 26 March 2024 (09:50:10 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1089.v1

White Paper Textile Fibre Recycling Technologies

Birgit Stubbe, Stijn Van Vrekhem, Sofie Huysman, Rémi G. Tilkin, Isabel De Schrijver, Myriam Vanneste

Subject: Chemistry And Materials Science, Paper, Wood And Textiles Keywords: textile recycling; fibre-to-fibre recycling; textile sorting; textile disassembly; digital textile platforms

Online: 17 November 2023 (08:49:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0999.v1

Nocturnal Glucose Profile according to Timing of Dinner Rapid Insulin and Basal and Rapid Insulin Type: An Insulclock® Connected Insulin Cap-Based Real-World Study

Fernando Gómez-Peralta, Xoan Valledor, Cristina Abreu, Elsa Fernández-Rubio, Laura Cotovad, Pedro Pujante, Sharona Azriel, Jesús Pérez-González, Alba Vallejo, Luis Ruiz-Valdepeñas, Rosa Corcoy

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Ultrarapid insulin; second-generation basal insulin; nocturnal hyperglycemia; nocturnal hypo-glycemia; connected insulin pen cap

Online: 15 May 2024 (12:29:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0217.v1

ERNet: A Rapid Road Cracks Detection Method from Low-Altitude UAV Remote Sensing Image

Zexian Duan, Jiahang Liu, Xinpeng Ling, Jinlong Zhang, Zhiheng Liu

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: UAV remote sensing; Road Cracks; Semantic segmentation; Crack quantification; Edge detection

Online: 2 April 2024 (14:33:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0237.v1

Rapid Non-Destructive Detection Technology in the Field of Meat Tenderness: A Review

Yanlei Li, Huaiqun Wang, Zihao Yang, Xiangwu Wang, Wenxiu Wang, Teng Hui

Subject: Biology And Life Sciences, Food Science And Technology Keywords: meat; tenderness; non-destructive; intelligent (AI); spectroscopy

Online: 2 April 2024 (17:30:46 CEST)

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Meat serves as a vital component of the human diet, offering a rich source of protein and essential trace elements. China stands as both a significant producer and consumer of meat on a global scale, leading the world in terms of total meat production and consumption. As the standard of living within the country continues to rise, there is a growing emphasis on the nutritional value and safety of meat consumption. The tenderness of meat is a critical factor influencing its overall quality. Traditionally, tenderness has been assessed through shear force testing, a method that is inherently destructive, inefficient, and results in considerable sample wastage. In light of these drawbacks, non-destructive testing techniques have emerged as a preferred alternative, promising greater accuracy, efficiency, and convenience without compromising the integrity of the samples. This paper delves into the application of five advanced non-destructive testing technologies in the realm of meat tenderness assessment. These include near-infrared spectroscopy, hyperspectral imaging, Raman spectroscopy, airflow optical fusion detection, and nuclear magnetic resonance detection. Each technology is scrutinized for its respective strengths and limitations, providing a comprehensive overview of their current utility and potential for future development. Moreover, the integration of these techniques with the latest advancements in artificial intelligence (AI) technology is explored. The fusion of AI with non-destructive testing offers a promising avenue for the development of more sophisticated, rapid, and intelligent systems for meat tenderness evaluation. This integration is anticipated to significantly enhance the efficiency and accuracy of quality assessment in the meat industry, ensuring a higher standard of safety and nutritional value for consumers. The paper concludes with a set of technical recommendations aimed at guiding the future direction of non-destructive, AI-enhanced meat tenderness detection, thereby contributing to the ongoing improvement of the meat industry in China and beyond.

Preprint ARTICLE | doi:10.20944/preprints202408.0671.v1

Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis

Yu Jeong Kim, JEONG HO CHANG

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: Lipase, Immobilization; Magnetic nanoparticles; Lipolysis; Acylation; Hydrolysis

Online: 9 August 2024 (12:11:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0262.v1

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Ju Huang, Ligong Zhai, Junyin Wang, Xiaotian Sun, Baoshi Wang, Zhaohui Wei

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Pseudomonas aeruginosa; Pseudomonas fragi; simultaneous detection; droplet digital PCR

Online: 3 April 2024 (16:17:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0705.v2

CrackScopeNet: A Lightweight Neural Network for Rapid Cracks Detection on Resource-Constrained Drone Platforms

Tao Zhang, Liwei Qin, Quan Zou, Liwen Zhang, Rongyi Wang, Heng Zhang

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Computer vision; Crack detection; Drone platforms; Semantic segmentation; Lightweight neural network

Online: 10 July 2024 (10:52:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0604.v2

RT-qPCR Diagnostics: The “Drosten” Sars-Cov-2 Assay Paradigm

Stephen Bustin, Sara Kirvell, Jim F Huggett, Tania Nolan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID-19; reverse transcription; qPCR; SARS-CoV-2; molecular diagnosis

Online: 3 August 2021 (15:34:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0704.v1

Rapid Visual Screening Feature Importance for Seismic Vulnerability Ranking via Machine Learning and SHAP values

Ioannis Karampinis, Lazaros Iliadis, Athanasios Karabinis

Subject: Engineering, Civil Engineering Keywords: Rapid Visual Screening; Explainable AI; Feature Importance; SHAP

Online: 12 February 2024 (14:53:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0299.v1

Optimizing Droplet Digital PCR for Environmental Samples

Vasilis Kokkoris, Eric Vukicevich, Andrew Richards, Corrina Thomsen, Miranda Hart

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ddPCR, droplet digital PCR, environmental samples, environmental DNA, soil microbiology

Online: 12 April 2021 (12:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0203.v1

Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken

Subject: Biology And Life Sciences, Insect Science Keywords: Tungiasis; Tunga penetrans; molecular entomology; DNA isolation; Phusion® polymerase; FIREpol® Taq polymerase; low-cost PCR,

Online: 10 November 2022 (11:19:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202004.0216.v1

Development of a Field-deployable RT-qPCR Workflow for COVID-19 Detection

Raphael Nyaruaba, Bo Zhang, Caroline Muema, Elishiba Muturi, Greater Oyejobi, Jin Xiong, Bei Li, Zhengli Shi, Caroline Mwaliko, Junping Yu, Xiaohong Li, Hongping Wei

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: COVID-19; SARS-CoV-2; field work; community; diagnosis; rapid detection; inactivation; RT-qPCR

Online: 14 April 2020 (08:41:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1709.v1

A Lateral-Flow Device for the Rapid Detection of Scedosporium Species

Genna E. Davies, Christopher R. Thornton

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Scedosporium; scedosporiosis; monoclonal antibody; biomarker; lateral-flow device; enzyme-linked immunosorbent assay

Online: 28 March 2024 (06:56:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.0705.v1

Rapid Growth of Population, GDP, and Built-up Land Exposed to High Seismic Hazard in Large Cities, China

Huiduo Wang, Qiang Dong, Weijiang Li, Jiahong Wen, Qing Wu, Yanming Chen, Jianli Liu, Li Li

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Earthquake; Exposure; High Seismically Hazardous Zone; Large City; China

Online: 11 June 2024 (15:40:05 CEST)

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In the context of rapid urbanization, the spatiotemporal patterns of socio-economic exposed to high seismic hazard in large cities lacks quantitative assessment, thus, it is difficult to understand the evolution of earthquake catastrophe risk there. In this paper, exposure change rate, and absolute and relatively exposure indices were proposed to quantitatively study the changes of spatiotemporal exposure to high seismic hazard in urban areas of large cities (i.e., permanent residents in urban areas more than 1 million) in Chinese mainland, using a set of multi-temporal population, GDP, and built-up datasets and a seismic hazard zoning map. The results show that, 64 out of 78 large cities are entirely or partially located in high seismically hazardous zones. Although the urban areas of large cities in high seismically hazardous zones (HSHUAs) cover only 1.73% (167177 km²) of mainland China, their share of population, GDP, and built-up land amounted to 18.14%, 30.24% and 24.55%, respectively, in 2015. Unprecedented urbanization has led to a dramatic exposure growth in HSHUAs between 1990 and 2015. The population, GDP, and built-up land in HSHUAs increased by 110 million people (with an average annual growth rate of 2.37%), 273.71 trillion USD(average annual growth rate of 11.15%), and 10593 km2 (average annual growth rate of 2.02%), respectively. HSHUAs experienced much faster population and GDP growth than the whole country, while relatively slower growth in built-up land. Exposure growth in HSHUAs also was varied for different types of large cities. Megacities had a higher proportion of increased GDP and population in HSHUAs, induced by industry and population agglomeration. Whereas, Type II large cities had a higher proportion of increased built-up land in HSHUAs, driven mainly by urban sprawl. Our analysis indicates that, for large cities under rapid urbanization, it is an urgent need to strengthen resilient urban planning, renewal and construction.

Preprint REVIEW | doi:10.20944/preprints202403.1182.v1

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Laura Sancha Domínguez, Ana Cotos Suárez, María Sánchez Ledesma, Juan Luis Muñoz Bellido

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: dPCR; digital PCR; infection; bacteria; virus; parasites

Online: 20 March 2024 (08:24:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1328.v1

Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of Bacteroides fragilis

Bakhtiyar Mahmood, Anna Paunkov, Malgorzata Kupc, Katalin Burián, Erzsébet Nagy, David Leitsch, József Sóki

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Bacteroides, metronidazole, nim, resistance mechanism, RT-qPCR

Online: 17 January 2024 (13:06:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202102.0525.v1

Exploring New Scaffolds for the Dual Inhibition of HIV-1 Rt Polymerase and Ribonuclease Associated Functions

Rita Meleddu, Angela Corona, Simona Distinto, Filippo Cottiglia, Serenella Deplano, Lisa Sequeira, Daniela Secci, Alessia Onali, Erica Sanna, Francesca Esposito, Italo Cirone, Francesco Ortuso, Stefano Alcaro, Enzo Tramontano, Peter Matyus, Elias Maccioni

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: HIV-RT; ribonuclease H; dual inhibitors; docking; putative binding

Online: 23 February 2021 (15:51:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.0931.v1

Rapid Monitoring of Scale Precipitation and Inhibition in Geothermal Fluid Using Optical Fiber Sensor Based on Surface Plasmon Resonance

Ai Hosoki, Kifuyu Sugiura, Takuya Okazaki, Heejun Yang, Hideki Kuramitz, Akira Ueda, Amane Terai

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: Geothermal scale sensor, Optical fiber, Surface plasmon resonance (SPR), Real-time monitoring

Online: 13 August 2024 (13:16:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1022.v1

Resilience Mechanisms and Coping Strategies for Forcibly Displaced Youth Experiencing Social Isolation: An Exploratory Rapid Review

Akm Alamgir, Christopher Kyriakides, Andrew Johnson, Gemechu Abeshu, Bay Bahri, Miles Abssy

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Coping mechanism; Ecology of Resilience; Refugee well-being; Youth Mental Health; Acculturation

Online: 14 August 2024 (12:26:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0068.v1

Creating Family-Centred Support for Preschoolers with Developmental Disabilities in Low-Income Countries: A Rapid Review to Guide Practitioners

Roy McConkey

Subject: Public Health And Healthcare, Health Policy And Services Keywords: disability; preschool; children; family-centred; community-based; low income; inclusion; healthcare; education

Online: 2 April 2024 (10:06:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0762.v1

High-Throughput Drug Stability Assessment via Biomimetic Metalloporphyrin Catalyzed Reactions Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry (LA-REIMS)

András Marton, Zsombor Márton Mohácsi, Balázs Decsi, Balázs Csillag, Júlia Balogh, Richárd Schäffer, Tamás Karancsi, György Tibor Balogh

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: high-throughput screening; metabolic stability; ambient mass spectrometry; LA-REIMS; biomimetic metalloporphyrin; lead profiling; in vitro models; drug metabolism

Online: 12 June 2024 (14:15:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1390.v1

Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection

Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: droplet Digital PCR; canine distemper virus; molecular diagnosis

Online: 18 September 2024 (10:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1177.v1

A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis

Thaís Ellen Chaves Gomes, Victor Coutinho Bastos, Douglas Boniek, Mário Romañach, Fernanda Faria Rocha, Roberta Rayra Martins Chaves, Ricardo Santiago Gomez

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Aspergillosis; Aspergillus; PCR; Diagnosis

Online: 15 August 2024 (14:30:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0326.v1

Molecular Characterization of Termitomyces Protoclones through PCR and RFLP Based DNA Typing

Pijush Mallick, Sobhan Roy Chowdhury, Dipanwita Roy, Subhash Kanti Roy, Samir Ranjan Sikdar

Subject: Biology And Life Sciences, Horticulture Keywords: DNA markers; edible mushroom; genome stability; protoplast regeneration

Online: 25 April 2018 (08:26:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0912.v2

Comparative Analysis of Rapid and Less Invasive Methods for A2A2 Dairy Cattle Genotyping and A2 Milk Purity Detection

Leandra Oliveira Xavier Albiero, Rafaela Ansiliero, Aniela Pinto Kempka

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: A2A2 dairy cows; Genotyping; ELISA; LFIA; Comparison of methods; Facilitated analysis

Online: 14 August 2024 (11:56:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201611.0125.v1

Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype

Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe, Tamás Tereh, Nóra Kovács, Anita Hidasi, Barbara Bánkuti, Krisztina Buzás, Frederic Lemnitzer, Zsolt Ruzsics, Susan Szathmary, Hans Wolf, Dániel Salamon, János Minárovits, Hans-Helmut Niller

Subject: Biology And Life Sciences, Virology Keywords: Lamin, EBV latency, transformation, epigenetic regulation, activated B cell

Online: 24 November 2016 (17:53:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0980.v1

Data-Driven AI Model for Turbomachinery Compressor Aerodynamics Enabling Rapid Approximation of 3D Flow Solutions

Marcel Aulich, Georgios Goinis, Christian Voß

Subject: Engineering, Mechanical Engineering Keywords: AI for 3D CFD; turbomachinery; compressor design; aerodynamic optimization; transformer network; deep neural network

Online: 15 May 2024 (10:24:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1653.v1

Development of a Rapid Detection Method for Ethylene Glycol and Glycolic Acid in Feline Samples: A Response to Increasing Antifreeze Poisoning Incidents in Korea

HyunYoung Chae, Jae Won Byun, Go-Eun Shin, Kyunghyun Lee, Ah-Young Kim, Bok-Kyung Ku, Md Akil Hossain, Tae-Wan Kim, JeongWoo Kang

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Antifreeze poisoning; Ethylene glycol; Glycolic acid; Rapid determination method

Online: 21 September 2024 (06:15:31 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202204.0104.v1

Statistical Modelling and Experimental Design for the Validation of Droplet Digital PCR Methods

Steffen Uhlig, Christopher Weidner, Bertrand Colson

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Method validation; droplet digital PCR; orthogonal factorial design; variance components; Poisson assumption; cloglog model; target DNA copies per droplet; Monte Carlo; prediction interval

Online: 12 April 2022 (08:46:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0279.v1

COVID19 PCR Testing and the Unprecedented Institutional Response in a Low-Resource Setting

Akintunde Orunmuyi, Olukemi Adekanmbi, Adeola Fowotade, Akindele Adebiyi, Adekunle Adeniji, Olukemi Amodu, E. Oluwabunmi Olapade-Olaopa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID19; sub-Sahara Africa; PCR testing; Capacity building

Online: 13 July 2020 (06:32:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201609.0001.v1

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Shan-Ling Hsu, Jai-Hong Cheng, Ching-Jen Wang, Jih-Yang Ko, Chih-Hsiang Hsu

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: protein-disulfide isomerase-associated 3; osteoarthritis; extracorporeal shockwave therapy; 1α,25-Dihydroxyvitamin D3 signaling pathway; two dimensional electrophoresis

Online: 1 September 2016 (10:47:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0156.v1

Composite Dynamic Double Network Hydrogels with Rapid Self-healing, Stretchable, Moldable and Antibacterial Properties based on PVA/ε-Poly-L-lysine/Hyaluronic Acid

Na Sun, Xiangnan Liu, Wenqi Lv, Chunlin Xu, Ailing Zhang, Panpan Sun

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: double network hydrogels; self-healing; antibacterial activity

Online: 3 September 2024 (10:01:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202002.0099.v1

The Determination of HIV-1 RT Mutation Rate, Its Possible Allosteric Effects, and Its Implications on Drug Resistance

Joshua Yi Yeo, Ghin Ray Goh, Chinh Tran-To Su, Samuel Ken-En Gan

Subject: Biology And Life Sciences, Virology Keywords: retroviruses; HIV-1; reverse transcriptase; mutation rate; drug resistance, allostery

Online: 7 February 2020 (11:50:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1505.v1

Diagnostic Accuracy of Multiplex PCR in Early Onset Neonatal Sepsis

Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: neonatal sepsis; early onset sepsis; multiplex PCR; infection; neonate; diagnosis; pathogen detection

Online: 24 October 2023 (08:11:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0559.v1

Optimization of RT-QuIC Assay Duration for Screening Chronic Wasting Disease in White-Tailed Deer

Gokhan Yilmaz, Tamara Morrill, Cian Ward, Gordon Mitchell, Andrei Soutyrine, Hanhong Dan, Min Lin, Jiewen Guan

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: RT-QuIC; Chronic Wasting Disease; Diagnostics; Optimization

Online: 7 September 2023 (15:45:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0839.v1

A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder

Yuanjun Wen, Shuqin Huang, Hongtao Lei, Xiangmei Li, Xing Shen

Subject: Biology And Life Sciences, Food Science And Technology Keywords: goat milk powder; CRISPR/Cas12a; adulteration; simultaneous detection; food fraud

Online: 12 April 2024 (08:00:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1839.v1

Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in Escherichia coli

Isidoro López, Fátima Otero, María del Carmen Fernández, Germán Bou, Jaime Gosálvez, José Luis Fernández García

Subject: Medicine And Pharmacology, Other Keywords: antibiotic combination; bacterial nucleoid; DNA fragments; rapid antibiogram; quinolone; beta-lactam

Online: 28 May 2024 (10:47:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Biology And Life Sciences, Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2272.v1

The Hypocholesterolemic Potential of the Edible Algae Fucus Vesiculosus: Proteomic and Quantitative PCR Analysis

Rebeca André, Rita Pacheco, Ana Catarina Alves, Hugo M. Santos, Mafalda Bourbon, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Fucus vesiculosus; cholesterol synthesis; cholesterol excretion; , Ezetimibe; , NPC1L1; ABCG5; qRT-PCR; mRNA

Online: 31 May 2023 (14:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0196.v1

SARS-CoV-2 PCR Persistence and Duration of COVID-19 Symptoms in Health Professionals

Tatiana Schäffer Gregianini, Ludmila Fiorenzano Baethgen, Richard Steiner Salvato, Ivone Andreatta Menegolla, CEVS COVID-19 Team, Ana Beatriz Gorini da Veiga

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: SARS-CoV-2; PCR persistent positive; respiratory infection; COVID-19

Online: 12 December 2022 (09:04:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0600.v1

Real-Time Monitoring of SARS-CoV-2 Variants in Oklahoma Wastewater through Allele-Specific RT-qPCR

Kristen Shelton, Gargi N Deshpande, Gilson J Sanchez, Jason Vogel, A Caitlin Miller, Gabriel Florea, Erin R Jeffries, Kara B. De Leon, Bradley Stevenson, Katrin Gaardbo Kuhn

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: wastewater surveillance; SARS-CoV-2; variant; RT-qPCR; sequencing; early warning; demography; Oklahoma

Online: 9 September 2024 (08:42:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0581.v1

Looking into the Quantification of Forensic Samples with Real-Time PCR

Ugo Ricci, Dario Ciappi, Ilaria Carboni, Claudia Centrone, Irene Giotti, Martina Petti, Alice Brogi, Elisabetta Pelo

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: DNA amplification; polymerase chain reaction; DNA quantification; forensic DNA

Online: 9 May 2024 (10:39:14 CEST)

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The quantification of human DNA extracts from forensic samples plays a key role in the forensic genetics process. Quantification guarantees maximum efficiency and avoids repeated analyses, over amplified samples, or unnecessary examinations. Here, we present our experience using an extremely sensitive real-time-based method with the Quantifiler® Trio DNA quantification kit, which we have explored in the ISO/IEC17025 accredited test validation process conducted in our laboratory. In this study, our focus was on establishing a minimum threshold beyond which DNA typing is unnecessary, by defining the correlation of negative quantification results with the absence of genetic profiles resulting from DNA typing. We investigated the results in the range in which the technique is used (50-0.005 ng/μl). In the validation process of the internally accredited method, we used data from the commercial quantification system, based on the average values of the standard curve using a wide range of samples, including artificially degraded samples. This method was extensively used continuously for nine years to quantify the DNA extract coming from a wide range of samples from criminal cases. The validation allowed us to verify the limits of the technique, especially in the use of the minimum quantities necessary to obtain genetic profiles acceptable for sending to the Italian DNA database. This optimization of the protocol for analysing forensic samples allows us to optimise the protocol for analysing forensic samples. However, the technique is used in our laboratory with a completely manual preparative phase, and we have decided to investigate the contribution of human error more accurately. Thus, in our experience, we have found that it is preferable to use quantification ranges rather than exact limits before deciding how to analyse the extracts via PCR. In addition, here we present some preliminary data related to the analysis of certain samples that initially appeared negative during quantification, applying a concentration method for maximum yield from forensic samples of judicial interest. Our aim is to ensure that no source of evidence is lost in the reconstruction of a criminal event.

Preprint ARTICLE | doi:10.20944/preprints202111.0210.v1

Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System

Shang Wang, Ying Tang, Jiehong Wei, Shan Wang, Guoyu Peng, Yuqing Li, Song Wu

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: CRISPR-Cas, Nucleic acid detection, Klebsiella pneumonia

Online: 11 November 2021 (12:57:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0189.v1

Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification

Lorena G. Caligiuri, Adolfo E. Sandoval, Jose C. Miranda, Felipe A. Pessoa, María S. Santini, Oscar D. Salomón, Nagila F. C. Secundino, Christina McCarthy

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sand fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia

Online: 17 December 2018 (10:41:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1114.v1

Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method

Andrew Chen, Duy P. Le, Linda J. Smith, Dinesh Kafle, Elizabeth A. B. Aitken, Donald M. Gardiner

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Upland cotton; pathogenicity; fungal effectors; Fusarium wilt; black root rot; Secreted In Xylem effector genes; endophyte; Translation elongation factor-1 alpha subunit

Online: 14 September 2024 (08:49:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202101.0264.v1

Measuring Time-Varying Effective Reproduction (Rt) Numbers for COVID-19 and Their Relationship with Movement Control Order (MCO) in Malaysia

Musa Kamarul Imran, Wan Nor Ariffin, Mohd Mohd Hafiz, Subhi Jamiluddin, Noor Atinah Ahmad, Chen Xin Wee, Tengku Muhammad Hanis, Awang Bulgiba

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Reproduction number; COVID-19; movement control order; epidemic curve

Online: 14 January 2021 (09:41:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1144.v1

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Gabriel S.M. Sousa, Rodrigo S. De Oliveira, Alex B. De Souza, Ruan C. Monteiro, Elaine P. T. E. Santo, Luciano C. Franco Filho, Denison L.O. Moraes, Sarah R. De Sá, Silvia H. M. Da Silva

Subject: Biology And Life Sciences, Other Keywords: chromoblastomycosis; phaeohyphomycosis; PCR-Multiplex; Herpotrichiellaceae; sequencing; phenotypic; molecular biology.

Online: 19 March 2024 (12:35:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1348.v1

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Pallavi Upadhyay, Jairus Reddy, Teddie Proctor, Oceane Sorel, Harita Veereshlingam, Manoj Gandhi, Vijay Singh

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Respiratory tract infections; COVID-19; influenza-like illness; multiplex PCR; syndromic panels

Online: 18 May 2023 (14:10:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0740.v1

Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow

Zachary P Morehouse, Caleb M Proctor, Gabriella L Ryan, Rodney J Nash

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Virus Detection; Viral Diagnostics; Diagnostics; SARS-CoV-2; Coronavirus; PCR

Online: 31 July 2020 (09:31:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0684.v2

How Adversarial Rem-Dreams May Facilitate Creativity, and Why We Become Aware of Them

Nicolas Deperrois, Mihai A. Petrovici, Jakob Jordan, Lukas S. Huber, Walter Senn

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: rapid-eye-movement; sleep; learning

Online: 10 May 2024 (13:24:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0664.v1

Practical and Rapid Motor Sizing Technigue using Existing Electrical Motor

YongChul Kim, JungHo Han, Ju Lee

Subject: Engineering, Electrical And Electronic Engineering Keywords: permanent magnet synchronous motor(PMSM); motor sizing; finite element method; FEM; electric power steering motor(EPS Motor); magnetic flux loss; parameter; cogging torque; torque ripple; toruqe-speed

Online: 11 October 2023 (04:52:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0425.v1

Impact of wind generation in the transformation centers of low voltage distribution networks using Opal-RT 5600

Javier Cabrera, Carlos Guerrero, Esteban Franco, Gabriela Araujo

Subject: Engineering, Electrical And Electronic Engineering Keywords: Wind energy; voltage profile; distribution network; simulation

Online: 6 June 2023 (09:35:15 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202111.0549.v1

PCR, PLS, or OPLS Evaluation of different regression techniques for hypothesis generation

Avani Ahuja

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Principal Component Regression, Partial Least Squares, Orthogonal Partial Least Squares, multivariate regression, hypothesis generation, Parkinson’s disease

Online: 29 November 2021 (15:42:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1200.v1

Assessment of Rapid Antigen Diagnostic Tests at Mass Events: Identifying Optimal Floor Plan Configurations for Enhanced Efficiency

Anas A Khan, Ahmad F Turki

Subject: Public Health And Healthcare, Health Policy And Services Keywords: COVID-19 Testing; Queueing Theory; M/M/c Model; Mass Gatherings; Operational Efficiency; Agile Management; System Throughput; Public Health Emergency Management; Antigen Testing; Process Optimization

Online: 16 August 2024 (04:53:00 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202306.2007.v1

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega, Klaudia Babisova, Michaela Hyblova, Nikola Janostiakova, Peter Sabaka, Pavol Janega, Gabriel Minárik

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Online: 29 June 2023 (02:12:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.2009.v1

Rapid Antibacterial Activity Assessment of Chimeric Lysin

Jin-Mi Park, Jun-Hyun Kim, Gun Kim, Hun-Ju Sim, Sun-Min Ahn, Kang-Seuk Choi, Hyuk-Joon Kwon

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Staphylococcus aureus; chimeric lysins; cell-free expression system; linker optimization; SH3 shuffling; antibacterial activity ranking

Online: 29 January 2024 (13:07:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.2134.v1

Rapid Thermal Processing of Kesterite thin films

Maxim Ganchev, Stanka Spasova, Taavi Raadik, Arvo Mere, Mare Altosaar, Enn Mellikov

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: photovoltaics, multinary chalcogenides, thermal annealing; Kesterite, XRD

Online: 3 July 2023 (10:47:25 CEST)

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Working Paper ARTICLE

Three Rapid Methods for Averaging GPS Segments

Jiawei Yang, Radu Mariescu-Istodor, Pasi Fränti

Subject: Engineering, Electrical And Electronic Engineering Keywords: GPS trajectories; segment averaging; sequence averaging; HC-SIM

Online: 14 July 2019 (09:14:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202106.0320.v1

Inherent Bias of SARS-CoV-2 RNA Quantification for Wastewater Surveillance Due to Variable RT-qPCR Assay Parameters

Aaron Bivins, Devrim Kaya, Kyle Bibby, Stuart Simpson, Stephen Bustin, Orin Shanks, Warish Ahmed

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: RT-qPCR; assay validity; standard curve; quality assurance; quality control; wastewater surveillance

Online: 11 June 2021 (14:10:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0284.v1

Designing and Validation of a Droplet Digital PCR Procedure for Diagnosis and Accurate Quantification of Nervous Necrosis Virus

Sandra Souto, José G. Olveira, Carmen López-Vázquez, Isabel Bandín, Carlos P. Dopazo

Subject: Biology And Life Sciences, Virology Keywords: VNNV; Diagnosis; Validation; dPCR; droplet digital PCR.

Online: 3 August 2023 (10:44:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0359.v1

Laser Sensing and Chemometric Analysis for Rapid Detection of Oregano Fraud

Luca Fiorani, Antonia Lai, Adriana Puiu, Florinda Artuso, Claudio Ciceroni, Isabella Giardina, Fabio Pollastrone

Subject: Physical Sciences, Applied Physics Keywords: quantum cascade laser application; laser spectroscopy; photoacoustic technique; multivariate calibration; agrofood chain; food fraud

Online: 6 July 2023 (07:08:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0434.v1

How Do Mass Rapid Transit and Convenience Stores Affect Housing Prices?

Qian Qingshan

Subject: Business, Economics And Management, Economics Keywords: Housing Prices; MRT; Prediction; Machine Learning

Online: 27 October 2022 (11:12:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1225.v1

Exploration of Temperature-Induced Quantum Proton Tunneling Probability in PCR-Mediated DNA Amplification

Joseph De Luna

Subject: Physical Sciences, Biophysics Keywords: genetics; molecular biology; biophysics; quantum mechanics

Online: 19 September 2023 (05:18:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1033.v2

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Meynard R. Austria, Jon Patrick T. Garcia, Lemmuel L. Tayo, Alvin R. Caparanga, Bonifacio Jr T. Doma

Subject: Engineering, Bioengineering Keywords: polymerase chain reaction (PCR); Computational Fluid Dynamics (CFD); Plasmodium falciparum; microfluidic chip design

Online: 10 August 2023 (10:30:52 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202211.0436.v1

Multiplex PCR Detects Respiratory Viruses at A Much Higher Rate in Kawasaki Disease

Masayuki Nagasawa, Teruyoshi Shimoyama, Sayuri Hashimoto, Ryuichi Nakagawa, Haruna Yokoyama, Mari Okada, Tomohiro Udagawa, Akihiro Oshiba

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Kawasaki disease; febrile convulsions; respiratory virus; filmarray; multiplex PCR

Online: 23 November 2022 (07:02:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.2169.v1

Spleen Swabs for Sensitive and High-Throughput Detection of African Swine Fever Virus by Real-Time PCR

Christopher Cafariello, Kalhari Goonewardene, Chungwon J Chung, Aruna Ambagala

Subject: Biology And Life Sciences, Virology Keywords: African swine fever; Diagnostics; Tissue homogenate; Spleen swab; Real-time PCR

Online: 26 July 2024 (16:49:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0184.v1

Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Result in a Decrease in PCR Efficiency

Olga A. Zasedateleva, Sergey A. Surzhikov, Viktoriya E. Kuznetsova, Valeriy E. Shershov, Victor E. Barsky, Alexander S. Zasedatelev, Alexander V. Chudinov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: C5-modified dUTPs; A and B family DNA polymerases; PCR amplification; X-ray structure; molecular modeling; non-covalent interactions

Online: 2 August 2023 (08:21:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1920.v1

Selection and Evaluation of Reference Genes for qRT-PCR Analysis in Amorphophallus konjac Based on Transcriptome Data

Yanli Liu, Chengcheng Zhang, Nunung Harijati, Ying Diao, Erxi Liu, Zhongli Hu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Amorphophallus; reference genes; RT-qPCR; gene expression

Online: 27 June 2023 (13:43:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0091.v1

Evidence-Based Evaluation of PCR Diagnostics for SARS-Cov-2 and The Omicron Variants by Sanger Sequencing

Sin Hang Lee

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: SARS-CoV-2; SARS-CoV; RT-PCR; Sanger sequencing; RT-qPCR; receptor-binding domain (RBD); N-terminal domain (NTD); Omicron; multi-allelic SNPs; false-positive

Online: 12 April 2022 (03:57:29 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.1427.v1

3D Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances

Ana Catarina Sousa, Rui Alvites, Bruna Lopes, Patrícia Sousa, Alícia Moreira, André Coelho, José Domingos Santos, Luís Atayde, Nuno Alves, Ana Colette Maurício

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: 3D printing; Additive manufacturing; Biomaterials; Biomedical applications; Bioprinting; Cellular therapies; Tissue regeneration

Online: 21 August 2024 (04:24:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0061.v1

Handheld Ultra-Fast duplex PCR assays and Lateral flow detection and identification of Leishmania parasites for cutaneous leishmanisis diagnosis

Insaf Bel Hadj Ali, Yusr Saadi Ben Aoun, Zeineb Hammami, Oumayma Rhouma, Ahmed Sahbi Chakroun, Ikram Guizani

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Cutaneous leishmaniases; Molecular diagnosis; Point of care; Leishmania; Molecular target; Palm PCR; Duplex PCR; Lateral Flow immunoassay

Online: 2 October 2023 (09:43:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0063.v1

Rapid Microbiological Assessment in Raw Milk. Validation of a Rapid Alternative Method for the Assessment of Microbiological Quality in Raw Milk.

Nicla Marri, Francesca Losito, Loris Le Boffe, Gilberto Giangolini, Simonetta Amatiste, Lorenza Murgia, Alyexandra Arienzo, Giovanni Antonini

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: raw milk; microbiological safety; microbiological quality; food safety; dairy; MBS method

Online: 3 August 2020 (08:07:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1994.v1

A Colorimetric RT-LAMP Detection of Multiple SARS-CoV-2 Variants of Concern and Lineages from Direct Nasopharyngeal Swab Samples without RNA Isolation

Santiago Enrique Werbajh, Luciana Larocca, Carolina Carrillo, Fabiana Stolowicz, Lorena Ogas, Sergio Pallotto, Solange Cassará, Lilia Mammana, Inés Zapiola, María Belén Bouzas, Adrián Alberto Vojnov

Subject: Biology And Life Sciences, Virology Keywords: COVID-19; SARS-CoV-2; LAMP

Online: 28 July 2023 (13:29:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1630.v1

Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli

Liena Zaidan, Inna Novodchuk, Alexandru Nica, Alexander H.Xu, Christopher Lloyd, Reza Karimi, Joe Sanderson, Michal Bajcsy, Mustafa Yavuz

Subject: Engineering, Electrical And Electronic Engineering Keywords: Graphene; FET; Biosensor; E.coli

Online: 24 June 2024 (08:43:50 CEST)

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Working Paper ARTICLE

Electronic Nose-Based Technique for Rapid Detection and Recognition of Moldy Apples

Jihua Wang, Gang Liang, Hui Tian, Jing Sun, Cihui Wan

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Electronic nose; apple; mildew; pattern recognition; artificial neural network; nondestructive examination

Online: 21 March 2019 (09:54:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.2000.v1

A Simple Inexpensive Alkaline Method for DNA Extraction from Environmental Samples for PCR Surveillance

Abdulkarim Shwani, Bin Zuo, Adnan Alrubaye, Jiangchao Zhao, Douglas Duane Rhoads

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: microbiome; bacteria; DNA extraction; poultry; swine; air sampling

Online: 30 November 2023 (15:40:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1939.v1

Actinobacillus pleuropneumoniae Serotypes by Multiplex PCR Identification and Evaluation of Lung Lesions in Pigs from Piedmont (Italy) Farms

Matteo Cuccato, Sara Divari, Silvia Ciaramita, Alessandra Sereno, Domenico Campelli, Pier Giuseppe Biolatti, Bartolomeo Biolatti, Francesco Meliota, Enrico Bollo, Francesca Tiziana Cannizzo

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Actinobacillus pleuropneumoniae; porcine pleuropneumonia; swine; respiratory diseases; serotyping; multiplex PCR

Online: 29 May 2024 (14:03:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0571.v1

Streamlined Two-Step Fragment Analysis PCR and Exome Sequencing of RFC1 for Diagnostic Testing of Suspected CANVAS Patients

Helena Jaklič, Ivana Babič Božović, Borut Peterlin, Anja Kovanda

Subject: Medicine And Pharmacology, Other Keywords: Cerebellar ataxia neuronopathy and vestibular areflexia syndrome (CANVAS); RFC1; genetic testing; genetic diagnostics, ; fluorescent PCR; repeat-primed PCR; repeat-expansion; fragment length analysis

Online: 11 March 2024 (05:36:41 CET)

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Working Paper ARTICLE

Detection of Peanut Allergen by Real Time PCR: Looking For the Suitable Detection Marker as Affected by Processing

Africa Sanchiz, Paulina Sánchez-Enciso, Carmen Cuadrado, Rosario Linacero

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Real-time PCR; peanut; food allergen; chloroplast marker; DNA isolation

Online: 16 June 2021 (11:33:10 CEST)

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