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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Biology And Life Sciences, Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0509.v1

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Antonio Tiberini, Ariana Manglli, Anna Taglienti, Ana Vučurović, Jakob Brodaric, Luca Ferretti, Marta Luigi, Andrea Gentili, Nataša Mehle

Subject: Biology And Life Sciences, Plant Sciences Keywords: ToBRFV; ToMMV; Solanum lycopersicum; Capsicum annum; leaves detection; seeds detections; performance criteria

Online: 31 December 2021 (11:12:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1845.v1

Determination Mitochondria's Quality Control of Parkinsonism Using Real-Time Reverse-Transcription Polymerase Chain Reaction

Al-Baraa Akram

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: RT: real time, PCR: polymerase chain reaction, WT: wild type, dNTP: deoxynucleotide triphosphate

Online: 28 April 2024 (10:36:25 CEST)

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Nuclear DNA mutations, mitochondrial DNA mutations, combined nuclear and mitochondrial DNA defects, and random occurrences are the main causes of mitochondrial disease. Black pigmentation loss is a major sign of Parkinsonism. Because of the loss of dopaminergic neurons in the substantia nigra, it drops to less than 80%. The degree of motor impairment was correlated with the amount of black pigmentation loss. After RNA extraction, reverse transcription is carried out because reverse transcriptase was used to create cDNA. One microliter of total RNA supplemented with IORT Buffer, dNTPs, random hexamers, Multi-Scribe Reverse Transcriptase, and nuclease-free was used to create cDNA. The following was how the reaction was carried out in a thermal cycler: 10 minutes at 25°C, 120 minutes at 37°C, 5 seconds at 85°C, and lastly 4°C. The data was expressed as a v-value, which shows how much the target gene is enriched in comparison to the reference gene. Earlier research using mice injected with functional HtrA2/Omi protease activity revealed similar outcomes, including induced up-regulation of CHOP, decreased mitochondrial function, and early brain cell aging because of an increase in mtDNA deletions. The cell viability of the genotypes exhibits a dmg concentration-dependent effect in these results, suggesting that high content ratios of drug treatment with 6-OHDA may be the cause of cellular content depletion. After 6-OHDA neurotoxin treatment, CHOP is up-regulated. Prior in vitro research has shown that treatment with a model based on 6-OHDA neurotoxin causes the activation of mitochondrial stress pathways, which in turn promotes the death of neurons. 6-OHDA reversibly inhibited complexes I and IV's activities in rat brain cells, which led to dopaminergic neurodegeneration, a defining feature of PD pathogens. As a result, the number of living cells will directly correlate with the dmg concentration, supporting the results of the current study. Real-time PCR can generally demonstrate that the removal of HtrA 2 results in a significant up-regulation of Hsp60 expression when compared to WT cells. This could be linked to a decreased threshold for mitochondrial stress in HtrA2 KO cells, which would explain why, following ADEP4 and ACP5 treatment, Hsp60 is significantly up-regulated in HtrA2 KO cells compared to WT cells.

Preprint ARTICLE | doi:10.20944/preprints202308.0387.v1

Acquisition of Humoral Immune Responses in Convalescent Japanese People with SARS-CoV-2 (COVID-19) Infection in 2021

Koshiro Monzen, Takanori Watanabe, Toshihiro Okabe, Hisakuni Sekino, Hironori Nakagami, Ryuichi Morishita

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2; Convalescent serum; Neutralizing antibody

Online: 4 August 2023 (12:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0151.v1

Functional Analysis of Forkhead Transcription Factor Fd59a in Spermatogenesis of Drosophila melanogaster

Ting Tang, Mengyuan Pei, Yanhong Xiao, Yingshan Deng, Yuzhen Lu, Xiao-Qiang Yu, Liang Wen, Qihao Hu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Spermatogenesis; Forkhead; FoxD; Testis; Apoptosis

Online: 5 March 2024 (05:01:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0826.v1

Potential Regulation of Iron Metabolism Gene Expression by DOF Transcription Factors in Dendrocalamus latiflorus

Peng-Kai Zhu, Mei-Xia Lin, Mei-Yin Zeng, Yu Tang, Xin-Rui Li, Tian-You He, Yu-Shan Zheng, Ling-Yan Chen

Subject: Biology And Life Sciences, Plant Sciences Keywords: DOF transcription factors; Iron Metabolism Related Genes; gene regulation; gene expression; Dendrocalamus latiflorus

Online: 10 July 2024 (10:14:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0030.v1

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi, Hussein Alburkat, Taisir Abdulelah Kadhim, Ammar M S Al-Helli, Mustafa Hamza Ghazi, Yasir Abdulameer N. Aldabbagh, Reem Numaan Talib, Sama Hussein Ali, Noor Hameed Imran, Ruaa Almohsen, Turki Muftin Saad, Pallavi Mandave

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: PCR; Salmonella Typhi; Widal Test; BHI

Online: 1 November 2022 (12:43:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints201611.0094.v1

Transcription Profile Unveils the Cardioprotective Effect of Aspalathin against Lipid Toxicity

Rabia Johnson, Phiwayinkosi V. Dludla, Christo J.F. Muller, Barbara Huisamen, M. Faadiel Essop, Johan Louw

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: diabetes mellitus; hyperglycemia; cardiomyopathy; lipid toxicity; polyphenols; aspalathin

Online: 17 November 2016 (11:19:37 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1523.v2

Highs and Lows in Calicivirus Reverse Genetics

Ángel L. Álvarez, Aroa Arboleya, Fábio A. Abade dos Santos, Alberto García-Manso, Inés Nicieza, Kevin P. Dalton, Francisco Parra, José M. Martín-Alonso

Subject: Biology And Life Sciences, Virology Keywords: calicivirus; reverse genetics; infectious clone; RNA virus

Online: 17 May 2024 (15:38:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1263.v2

A Method to Reverse Epimutations

Steven Olsen

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Homeostasis; HDEC; Hormesis; Epimutations; Susceptibility; Toxicogenomics

Online: 17 September 2024 (05:04:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.2129.v1

Immortalization of Human Primary CD8⁺ T cells by Inserting a Single Copy of Human Telomerase Reverse Transcriptase via CRISPR/Cas9

He Zhiyong, Kenneth Cole, Hua-Jun He

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Human CD8⁺ T cells; Immortalization; Telomerase reverse transcriptase (TERT); CRISPR/Cas9; CDKN2A; p16; Large DNA fragment insertion; Homologous recombination

Online: 29 August 2024 (09:20:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1854.v1

Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis

Xiu-Zhang Li, Yu-Ling Li, Jia-Shi Zhu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Differential transcription mating-type genes; mutant MAT1-1-1 protein; mutant α-pheromone receptor protein; genotypes of Ophiocordyceps sinensis; natural Cordyceps sinensis; transcriptional regulation; homothallism; pseudohomothallism; physiological heterothallism; hybridization

Online: 29 March 2024 (13:19:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0262.v1

Evaluation of Sensitivity and Applicability of a Droplet Digital Pcr Assay for Simultaneous Detecting Pseudomonas aeruginosa and Pseudomonas fragi in Foods

Ju Huang, Ligong Zhai, Junyin Wang, Xiaotian Sun, Baoshi Wang, Zhaohui Wei

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Pseudomonas aeruginosa; Pseudomonas fragi; simultaneous detection; droplet digital PCR

Online: 3 April 2024 (16:17:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1653.v2

The p53 tumor suppressor protein activates transcription of DUSP13 isoform from the alternative promoter in intron

Małgorzata Krześniak, Barbara Łasut-Szyszka, Agnieszka Będzińska, Agnieszka Gdowicz-Kłosok, Marek Rusin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: p53; MDM2; DUSP13; alternative promoter; dual-specificity phosphatase

Online: 26 February 2024 (16:55:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1740.v1

Predicting Clinical Response to Monoclonal TNF Inhibitors in Rheumatoid Arthritis: A Transcriptomic Approach Based on Transmembrane TNF Reverse Signaling and NRF2 Activation

Katy Diallo, Yannick Degboé, Michel Baron, Anais Bellin-Robert, Jean Frédéric Boyer, Arnaud Constantin, Adeline Ruyssen-Witrand, Benjamin Rauwel, Alain Cantagrel, Jean-Luc DAVIGNON

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Rheumatoid Arthritis, Anti-TNF, NRF2, Reverse Signaling, Biotherapy, Monoclonal Antibodies, Monocytes, Prediction.

Online: 29 February 2024 (14:46:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1426.v1

Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma

Jian Shi

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: glioblastoma (GBM); recurrent mechanism; bevacizumab (BVZ); E2F family

Online: 22 May 2024 (08:30:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202001.0294.v1

Virus-Like Particles: Applications in Reverse Vaccinology

Agustin De Ganzó, Mercedes Pastorini, Cristina Borio, Mario Lozano, Sandra Goñi

Subject: Biology And Life Sciences, Virology Keywords: VLPs; vaccines; reverse vaccinology; JUNV; vaccinology

Online: 25 January 2020 (00:55:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0256.v1

Algebraic Morphology of DNA--RNA Transcription and Regulation

Michel Planat, Marcelo M Amaral, Klee Irwin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Transcription factors; micro-RNAs; diseases; finitely generated group; $SL(2,\mathbb{C})$ character variety, algebraic surfaces

Online: 14 December 2022 (11:02:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.1736.v1

Screening the Performance of a Reverse Osmosis Pilot-Scale Process that Treats a Blended Feedwater Containing Nanofiltration Concentrate and Brackish Groundwater

Christopher R. Hagglund, Steven J. Duranceau

Subject: Engineering, Civil Engineering Keywords: nanofiltration; reverse osmosis; zero-liquid discharge; membrane concentrate; pilot-plant; brackish groundwater; iron sulfide; pyrite

Online: 25 June 2024 (09:27:19 CEST)

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A two-stage pilot plant study has been completed that evaluated the performance of a reverse osmosis (RO) membrane process for the treatment of a feedwater that consisted of a blend of nanofiltration (NF) concentrate and brackish groundwater. Membrane performance was assessed by monitoring the process operation, collecting water quality data, and documenting the blended feedwater’s impact on fouling due to a combination of, or solely caused by, plugging, scaling, microbiological, or organic means. Fluorescence and biological activity reaction tests were used to identify the types of organics and microorganisms present in the blended feedwater. Additionally, scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS) were used to analyze suspended matter that collected on the surfaces of cartridge filters used in the pilot’s pretreatment system. SEM and EDS were also used to evaluate solids collected on the surfaces of 0.45-µm silver filter pads after filtering known volumes of NF concentrate and RO feedwater blends. Water quality analyses confirmed that the blended feedwater contained little to no dissolved oxygen, and the blended feedwater was absent of a significant amount of particulate matter as defined by silt density index and turbidity measurements. However, water quality results suggested that the presence of sulfate, sulfide, iron, anaerobic bacteria, and humic acid organics likely contributed to the formation of pyrite observed on some of the membrane surfaces autopsied at the conclusion of pilot operations. It was determined that first-stage membrane productivity was impacted by the location of cartridge filter pretreatment; however, second-stage productivity was maintained with no observed flux decline during the entire pilot operation’s timeline. Study results indicated that the operation of an RO process treating a blend of NF concentrate and brackish groundwater could maintain a sustainable and productive operation that provided a practical zero-discharge process operation while the dilution of RO feedwater salinity would lower overall production costs.

Preprint ARTICLE | doi:10.20944/preprints202408.1531.v1

R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis

Qingwei Suo, Nianjuan Fang, Jianyan Zeng, Fulin Yan, Xi Zhu, Yi Wang, Wanting Yu, Junmin Chen, Aimin Liang, Yaohua Li, Jie Kong, Yuehua Xiao

Subject: Biology And Life Sciences, Plant Sciences Keywords: Gossypium hirsutum; R2R3 MYB transcription factor; fiber elongation; cell wall loosening; KCS

Online: 22 August 2024 (04:09:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0032.v1

Discovery of Benzisothiazolone Derivatives as Bifunctional Inhibitors of HIV-1 Reverse Transcriptase DNA Polymerase and Ribonuclease H Activities

Alondra Vázquez Rivera, Heather Donald, Mounia Alaoui-El-Azher, John J. Skoko, John S. Lazo, Michael A. Parniak, Paul A. Johnston, Nicolas Sluis-Cremer

Subject: Biology And Life Sciences, Virology Keywords: HIV-1; reverse transcriptase; polymerase; ribonuclease H; inhibitor; benzisothiazolone

Online: 3 June 2024 (07:56:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0299.v1

Optimizing Droplet Digital PCR for Environmental Samples

Vasilis Kokkoris, Eric Vukicevich, Andrew Richards, Corrina Thomsen, Miranda Hart

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ddPCR, droplet digital PCR, environmental samples, environmental DNA, soil microbiology

Online: 12 April 2021 (12:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1468.v1

Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR

Claudio A. Henriquez, Natalia Riquelme, Alejandra Larach, Ximena Besoain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Virus-viroid detection; Mixed infections; Comparison of diagnostic methods; CTV; HSVd; Bioamplification

Online: 19 January 2024 (07:52:21 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202404.0878.v1

How to Regress or Reverse Coronary Artery Disease

Dasaad Mulijono, Albert Manggading Hutapea, I Nyoman Ehrich Lister

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: plant-based diet; atherosclerotic; vulnerable plaque; supplements

Online: 15 April 2024 (04:12:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.1035.v1

Recent Advances in Reverse Water Gas Conversion Reaction

Changjian Zhou, Jiahao Zhang, Yuqing Fu, Hui Dai

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: CO2 conversion; CO; reverse water gas conversion reaction

Online: 17 October 2023 (09:35:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0213.v1

Influence Maximization Algorithm Based on Reverse Reachable Set

Gengxin Sun, Chih-Cheng Chen

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: social networks; influence maximization; information diffusion model; reverse reachable set; sub-modularity

Online: 8 February 2021 (14:18:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints201804.0005.v1

A Forward-Reverse Brascamp-Lieb Inequality: Entropic Duality and Gaussian Optimality

Jingbo Liu, Thomas A. Courtade, Paul W. Cuff, Sergio Verdú

Subject: Computer Science And Mathematics, Analysis Keywords: Brascamp-Lieb inequality; hypercontractivity; functional-entropic duality; Gaussian optimality; network information theory; image size characterization

Online: 2 April 2018 (06:02:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0203.v1

Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted From Soil Samples Containing PCR Inhibitor-Rich Material

Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken

Subject: Biology And Life Sciences, Insect Science Keywords: Tungiasis; Tunga penetrans; molecular entomology; DNA isolation; Phusion® polymerase; FIREpol® Taq polymerase; low-cost PCR,

Online: 10 November 2022 (11:19:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.1434.v1

ARE/Nrf2 Transcription System Involved in Carotenoid, Polyphenol, and Estradiol Protection from Rotenone‐Induced Mitochondrial Oxidative Stress in Dermal Fibroblasts

Aya Darawsha, Aviram Trachtenberg, Yoav Sharoni

Subject: Biology And Life Sciences, Aging Keywords: mitochondrial dysfunction; dermal fibroblasts; rotenone; reactive oxygen species (ROS); matrix metalloproteinase (MMP); collagen; antioxidant response element/Nrf2 (ARE/Nrf2); tomato extract; rosemary extract; estradiol

Online: 18 July 2024 (14:49:49 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1730.v1

NPAS2, A Circadian Hemeprotein and Gas-Responsive Transcription Factor

Emanuele Murgo, Tommaso Colangelo, Maria Marina Bellet, Francesco Malatesta, Gianluigi Mazzoccoli

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: NPAS2; circadian; rhythmicity; biological clock; PAS-domain

Online: 24 August 2023 (10:07:49 CEST)

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Preprint REVIEW | doi:10.20944/preprints202001.0243.v1

The DOF Transcription Factors in Seed and Seedling Development

Veronica Ruta, Chiara Longo, Andrea Lepri, Veronica De Angelis, Sara Occhigrossi, Paolo Costantino, Paola Vittorioso

Subject: Biology And Life Sciences, Plant Sciences Keywords: DOF proteins; DELLA proteins; seed germination; seedling development; seed maturation

Online: 21 January 2020 (11:16:52 CET)

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Preprint REVIEW | doi:10.20944/preprints202002.0458.v1

Tuning up Transcription Factors for Therapy

Attila Becskei

Subject: Biology And Life Sciences, Biophysics Keywords: LacI; Lac repressor; TetR; TAL-Effector; transcription activator-like effector; Cas9d; dissociation rate constant

Online: 29 February 2020 (09:13:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints201701.0088.v2

NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Adipose derived stem cell (ASC); Regenerative medicine; embryonic stem cell marker network

Online: 20 January 2017 (04:56:06 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1182.v1

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Laura Sancha Domínguez, Ana Cotos Suárez, María Sánchez Ledesma, Juan Luis Muñoz Bellido

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: dPCR; digital PCR; infection; bacteria; virus; parasites

Online: 20 March 2024 (08:24:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.2120.v1

Trypanosoma cruzi STIB980: A TcI Strain for Drug Discovery and Reverse Genetics

Anna Fesser, Sabina Beilstein, Marcel Kaiser, Remo S. Schmidt, Pascal Mäser

Subject: Biology And Life Sciences, Parasitology Keywords: Trypanosoma cruzi; genome sequencing; reverse genetics; drug efficacy testing

Online: 31 August 2023 (04:19:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0229.v1

Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen

Md. Mynul Hassan, Sung-Wook Nam

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: HPLC; NSAIDs; Isocratic; Short column; Drug mixture

Online: 9 July 2021 (15:23:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1852.v1

Symmetric Reverse $n$-Derivations on Ideals of Semiprime Rings

Shakir Ali, Ali Y. Hummdi, Naira N. Rafiquee, Vaishali Varshney, KokBin Wong

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: Semiprime ring; ideal; symmetric $n$-derivation; symmetric reverse $n$-derivation; derivation; trace of symmetric map

Online: 24 September 2024 (11:59:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0199.v1

Reverse Engineering of Radical Polymerizations by Multi-objective Optimization

Jelena Fiosina, Philipp Sievers, Gavaskar Kanagaraj, Marco Drache, Sabine Beuermann

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: polymerization reverse engineering; clustering; multi-objective optimization

Online: 5 February 2024 (07:10:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints201705.0123.v1

Abuse of Mobile Devices by Making Reverse Proxy Server

Dogukan Aydinli, Emre Koroglu, Volkan Erol

Subject: Computer Science And Mathematics, Computer Science Keywords: Mobile device threats; mobile device malware; reverse proxy server; cyber security; android security; ios security; abuse of local area network; DNS spoofing; DNS hijacking

Online: 16 May 2017 (13:23:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0180.v1

The Distal Promoter of the B438L Gene of African Swine Fever Virus Is Responsible for the mRNA Transcription of the B438L and the Alternatively Spliced B169L

Hongwei Cao, Hao Deng, Yanjin Wang, Diqiu Liu, Lian-Feng Li, Meilin Li, Dingkun Peng, Jingwen Dai, Jiaqi Li, Hua-Ji Qiu, Su Li

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: African swine fever virus; B438L promoter; transcription initiation; B169L protein

Online: 3 May 2024 (09:46:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1288.v1

Biocompatible and Biomimetic Minerals Serum Can Reverse Dental Erosion and Improve Tooth Shade Variation.

Silvia D’Agostino, Giulia Valentini, Junaid Rafique, Marco Dolci

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: biocompatible minerals; calcium silicate sodium phosphate; enamel; Erosion; hydroxyapatite; remineralization

Online: 18 August 2023 (08:12:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1390.v1

Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection

Victoria Iribarnegaray, Guillermo Godiño, Camila Larrañaga, Kanji Yamasaki, José Manuel Verdes, Rodrigo Puentes

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: droplet Digital PCR; canine distemper virus; molecular diagnosis

Online: 18 September 2024 (10:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1177.v1

A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis

Thaís Ellen Chaves Gomes, Victor Coutinho Bastos, Douglas Boniek, Mário Romañach, Fernanda Faria Rocha, Roberta Rayra Martins Chaves, Ricardo Santiago Gomez

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Aspergillosis; Aspergillus; PCR; Diagnosis

Online: 15 August 2024 (14:30:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0326.v1

Molecular Characterization of Termitomyces Protoclones through PCR and RFLP Based DNA Typing

Pijush Mallick, Sobhan Roy Chowdhury, Dipanwita Roy, Subhash Kanti Roy, Samir Ranjan Sikdar

Subject: Biology And Life Sciences, Horticulture Keywords: DNA markers; edible mushroom; genome stability; protoplast regeneration

Online: 25 April 2018 (08:26:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1525.v1

Environment Mapping Based Classification for Reverse Engineering Using Supervised Learning

Sukhyun Lim

Subject: Engineering, Electrical And Electronic Engineering Keywords: reverse engineering; classification; environment mapping; supervised learning

Online: 22 August 2024 (02:35:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1885.v1

Tubular Structure Segmentation via Multi-Scale Reverse Attention Sparse Convolution

Xueqiang Zeng, Yingwei Guo, Asim Zaman, Haseeb Hassan, Jiaxi Lu, Jiaxuan Xu, Xiaoqiang Miao, Huihui Yang, Anbo Cao, Yingjian Yang, Rongchang Chen, Yan Kang

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: cerebrovascular; airway; tubular structures; multi-scale; reverse attention; sparse convolution

Online: 26 May 2023 (07:58:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0364.v1

Reverse Flood Routing in an Open Channel Using Genetic Algorithm

Ali Azizipour, Seyed Mahmood Kashefipour, Ali Haghighi

Subject: Engineering, Automotive Engineering Keywords: generating missed hydrograph; genetic algorithm; Reverse Flood Routing; Karun River; numerical FASTER model

Online: 17 February 2021 (10:09:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0393.v1

Reverse Engineering to Study the Turin-Shroud Body-Image Formation

Riccardo Campion, Giulio Fanti

Subject: Engineering, Control And Systems Engineering Keywords: image analysis; Turin Shroud; body-image formation; energy propagation

Online: 18 October 2018 (03:55:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0031.v1

WRKY Transcription Factor Responses and Tolerance to Abiotic Stresses in Plants

Ziming Ma, Lanjuan Hu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: WRKY transcription factor; abiotic stress response and tolerance; target gene; plant growth and development

Online: 3 June 2024 (08:04:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0381.v2

Group Theory of Syntactical Freedom in DNA Transcription and Genome Decoding

Michel Planat, Marcelo M. Amaral, Fang Fang, David Chester, Raymond Aschheim, Klee Irwin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: DNA transcription factors; genes; finitely-generated groups; free groups; aperiodic order

Online: 10 January 2022 (11:52:04 CET)

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Preprint REVIEW | doi:10.20944/preprints202111.0370.v1

The Role of Selected Signaling Pathways and Transcription Factors in Chondrogenesis

Joseph A. Ayariga

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cartilage; transcriptional factors; Chondrogenesis

Online: 19 November 2021 (15:20:13 CET)

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During cartilage development, the lineage commitment and condensation of stem cells into chondrocytes and their differentiation involves a ubiquitous signaling cascades and huge numbers of transcriptional factors. The kinetic requirements and the stoichiometry for the expression of key transcriptional factors are relevant and must be met to form proper and functionally competent cartilage tissue. More interestingly also, an exact and precise spatio-temporal distribution of these molecules are as necessary in the proper tissue morphogenesis and patterning as the relevant physical conditions and micro environmental forces playing at the background during embryogenesis. A milestone of experimental achievements has been obtained over the years on several signaling pathways involved in cartilage development. Several fate determining transcriptional factors has also been investigated and determined with regards to the transition of stem cells (pluripotent, embryonic, etc.) into chondrocytes. These transcriptional factors serve as master controllers in chondrocytes proliferation and hypertrophy. Concerns that variability in signaling and transcriptional factors have detrimental effect on cartilage formation and could potentiate most cartilage related diseases have led most scientists to investigate the role of signaling molecules and transcriptional factors implicated in osteoarthritis, rheumatoid arthritis, and other cartilage degenerative diseases. On bases of spatio-temporal distribution of transcriptional factors, there exist functional overlaps, hence, it is difficult to draw a hard line of demarcation of roles at each point of the cell’s life, nonetheless, it is also markedly established that some factors are skewed to the chondrocyte’ survival and proliferation, and others known for their master’s role in the cell’s apoptotic, necrotic and senescence. Here we review some published works on selected signaling pathways and transcriptional factors that are preferentially expressed in chondrogenic cells and their role as major players in cartilage formation, cartilage diseases, along with some highlights of unique signaling molecules that are indispensable in cartilage tissue regeneration and management.

Preprint COMMUNICATION | doi:10.20944/preprints202312.0806.v1

Antiretroviral Promise: Amentoflavone and Nilotinib Strong Binding and Low Toxicity Against HIV-1 Reverse Transcriptase

IVAN VITO FERRARI

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: HIV-1 reverse transcriptase; pyrx program; docking approach; Pro-Tox-II; pkCSM

Online: 12 December 2023 (05:48:00 CET)

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Preprint REVIEW | doi:10.20944/preprints202106.0176.v1

Strategies to Inhibit Hepatitis B Virus Gene Transcription

Bingqian Qu, Richard J. P. Brown

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: chronic hepatitis B; covalently closed circular DNA; viral integration; transcription factor; nuclear receptor; transcriptional inhibitor; RNA interference

Online: 7 June 2021 (12:43:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202103.0066.v1

Multifarious Roles of GRAS Transcription Factors in Plants

Priya Kumari, Mrinalini Kakkar, Vijay Gahlaut, Vandana Jaiswal, Sanjay Kumar

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: GRAS protein, DELLA, Intrinsically Disordered Proteins, Arbuscular Mycorrhizal association, abiotic stress

Online: 2 March 2021 (10:01:42 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints201905.0062.v1

SCAND1 Suppresses CDC37 Gene Transcription by Repressing MZF1

Takanori Eguchi, Thomas L. Prince, Tien Manh Tran, Chiharu Sogawa, Benjamin J. Lang, Stuart K. Calderwood

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: SCAN zinc-finger; SCAND1; CDC37; MZF1; prostate cancer

Online: 6 May 2019 (12:10:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.1373.v1

Glioblastoma Standard of Care: Effects on Tumor Evolution and Reverse Translation in Preclinical Models

Louis T. Rodgers, John L Villano, Anika MS Hartz, Björn Bauer

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: glioblastoma; standard of care; resection; radiation therapy; temozolomide; recurrent tumor; reverse translation; preclinical models

Online: 20 June 2024 (03:29:51 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202204.0104.v1

Statistical Modelling and Experimental Design for the Validation of Droplet Digital PCR Methods

Steffen Uhlig, Christopher Weidner, Bertrand Colson

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Method validation; droplet digital PCR; orthogonal factorial design; variance components; Poisson assumption; cloglog model; target DNA copies per droplet; Monte Carlo; prediction interval

Online: 12 April 2022 (08:46:49 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0279.v1

COVID19 PCR Testing and the Unprecedented Institutional Response in a Low-Resource Setting

Akintunde Orunmuyi, Olukemi Adekanmbi, Adeola Fowotade, Akindele Adebiyi, Adekunle Adeniji, Olukemi Amodu, E. Oluwabunmi Olapade-Olaopa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID19; sub-Sahara Africa; PCR testing; Capacity building

Online: 13 July 2020 (06:32:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1390.v1

Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

Mirko Sonntag, Vanessa K. Elgeti, Yevhen Vainshtein, Lucca Jenner, Jan Mueller, Thorsten Brenner, Sebastian O. Decker, Kai Sohn

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sepsis; antimicrobial resistances; cell-free DNA; precision diagnostics; Next-Generation sequencing; suppression PCR; real-time diagnostics; nanopore sequencing

Online: 22 April 2024 (10:13:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1505.v1

Diagnostic Accuracy of Multiplex PCR in Early Onset Neonatal Sepsis

Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: neonatal sepsis; early onset sepsis; multiplex PCR; infection; neonate; diagnosis; pathogen detection

Online: 24 October 2023 (08:11:56 CEST)

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Preprint REVIEW | doi:10.20944/preprints202001.0199.v1

Role of Signal Transduction Pathways and Transcription Factors in Cartilage and Joint Diseases

Riko Nishimura, Kenji Hata, Yoshifumi Takahata, Tomohiko Murakami, Eriko Nakamura, Maki Ohkawa, Lerdluck Ruengsinpinya

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: osteoarthritis; rheumatoid arthritis; fibrodysplasia ossificans progressive; achondroplasia

Online: 18 January 2020 (09:31:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.1093.v1

AP2/ERF transcription factor responses and tolerates to abiotic stresses in plants

Ziming Ma, Lanjuan Hu, Wenzhu Jiang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: AP2/ERF transcription factor; abiotic stress; target gene, hormone signalling

Online: 14 December 2023 (11:25:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202008.0527.v1

RNA Polymerase and Transcription Mechanism: Forefront of Physicochemical Study as Chemical Reactions

Nobuo Shimamoto, Masahiko Imashimizu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: transcriptional regulation; reaction theory; prediction of promoters; one-dimensional diffusion; rate equation; detailed balance; antenna effect; physicochemical techniques

Online: 24 August 2020 (10:09:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0562.v1

Active and Stable MgAl₂O₄ and Ni(SA)/MgAl₂O4 Catalysts for the Reverse Water Gas Shift Reaction

Lingling Zhang, Qi Song, Yimeng Xing, Zhichun Si, Yuxiang Liu, Rui Ran, Xiaodong Wu, Duan Weng, Feiyu Kang

Subject: Chemistry And Materials Science, Biomaterials Keywords: MgAl2O4, Single atomic Nin+, RWGS, Stability, Reaction mechanism

Online: 24 May 2021 (10:50:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202007.0744.v1

DNA Processing in The Context of Non-Coding Transcription

Uthra Gowthaman, Desiré García-Pichardo, Yu Jin, Isabel Schwarz, Sebastian Marquardt

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: long non-coding RNA (lncRNA); RNA polymerase II (RNAPII) transcription; gene regulation; tandem transcriptional interference (tTI); antisense transcription; DNA processing

Online: 31 July 2020 (10:47:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2272.v1

The Hypocholesterolemic Potential of the Edible Algae Fucus Vesiculosus: Proteomic and Quantitative PCR Analysis

Rebeca André, Rita Pacheco, Ana Catarina Alves, Hugo M. Santos, Mafalda Bourbon, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Fucus vesiculosus; cholesterol synthesis; cholesterol excretion; , Ezetimibe; , NPC1L1; ABCG5; qRT-PCR; mRNA

Online: 31 May 2023 (14:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0196.v1

SARS-CoV-2 PCR Persistence and Duration of COVID-19 Symptoms in Health Professionals

Tatiana Schäffer Gregianini, Ludmila Fiorenzano Baethgen, Richard Steiner Salvato, Ivone Andreatta Menegolla, CEVS COVID-19 Team, Ana Beatriz Gorini da Veiga

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: SARS-CoV-2; PCR persistent positive; respiratory infection; COVID-19

Online: 12 December 2022 (09:04:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1016.v2

DNA Gene's Basic Structure as a Nonperturbative Circuit Quantum Electrodynamics: Is RNA Polymerase II the Quantum Bus of Transcription?

ANNIE RIERA LEAL, RAUL RIERA AROCHE, YVETH MARLENE ORTIZ GARCIA, ESLI CAMILA SANCHEZ MORENO, JOSE SAULO ENRIQUEZ CERVANTES, ANDREA CAROLINA MACHADO SULBARAN

Subject: Biology And Life Sciences, Biophysics Keywords: DNA gene's basic structure; circuit quantum electrodynamics; quantum bus of transcription

Online: 25 September 2024 (11:50:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1208.v1

An Investigation of Approaches for Enhanced Reverse Flotation of Refractory Hematite Ore

Zhongxian Wu, Hao Huang, Huaizhi Shao, Dongping Tao, Youjun Tao

Subject: Engineering, Mining And Mineral Processing Keywords: process mineralogy; hematite; quartz; reverse flotation; flotation mechanisms

Online: 16 June 2023 (10:00:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints201811.0061.v1

Versatility of Reverse Micelles: From Biomimetic Model to Nano(Bio)Sensors Design

Melania-Liliana Arsene, Ana-Maria Gurban, Maria-Luiza Jecu, Mihaela Doni

Subject: Engineering, Bioengineering Keywords: reverse micelles, biomimetic, confinement, water dynamics, nanoparticles, nano(bio) sensors

Online: 2 November 2018 (13:25:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints201710.0132.v1

Accelerated Benders’ Decomposition for Integrated Forward/Reverse Logistics Network Design under Uncertainty

Vahab Vahdat, Mohammad Ali vahdatzad

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: integrated forward/reverse logistics network; accelerated benders’ decomposition; two-stage stochastic programming

Online: 20 October 2017 (03:07:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0581.v1

Looking into the Quantification of Forensic Samples with Real-Time PCR

Ugo Ricci, Dario Ciappi, Ilaria Carboni, Claudia Centrone, Irene Giotti, Martina Petti, Alice Brogi, Elisabetta Pelo

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: DNA amplification; polymerase chain reaction; DNA quantification; forensic DNA

Online: 9 May 2024 (10:39:14 CEST)

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The quantification of human DNA extracts from forensic samples plays a key role in the forensic genetics process. Quantification guarantees maximum efficiency and avoids repeated analyses, over amplified samples, or unnecessary examinations. Here, we present our experience using an extremely sensitive real-time-based method with the Quantifiler® Trio DNA quantification kit, which we have explored in the ISO/IEC17025 accredited test validation process conducted in our laboratory. In this study, our focus was on establishing a minimum threshold beyond which DNA typing is unnecessary, by defining the correlation of negative quantification results with the absence of genetic profiles resulting from DNA typing. We investigated the results in the range in which the technique is used (50-0.005 ng/μl). In the validation process of the internally accredited method, we used data from the commercial quantification system, based on the average values of the standard curve using a wide range of samples, including artificially degraded samples. This method was extensively used continuously for nine years to quantify the DNA extract coming from a wide range of samples from criminal cases. The validation allowed us to verify the limits of the technique, especially in the use of the minimum quantities necessary to obtain genetic profiles acceptable for sending to the Italian DNA database. This optimization of the protocol for analysing forensic samples allows us to optimise the protocol for analysing forensic samples. However, the technique is used in our laboratory with a completely manual preparative phase, and we have decided to investigate the contribution of human error more accurately. Thus, in our experience, we have found that it is preferable to use quantification ranges rather than exact limits before deciding how to analyse the extracts via PCR. In addition, here we present some preliminary data related to the analysis of certain samples that initially appeared negative during quantification, applying a concentration method for maximum yield from forensic samples of judicial interest. Our aim is to ensure that no source of evidence is lost in the reconstruction of a criminal event.

Preprint ARTICLE | doi:10.20944/preprints202111.0210.v1

Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System

Shang Wang, Ying Tang, Jiehong Wei, Shan Wang, Guoyu Peng, Yuqing Li, Song Wu

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: CRISPR-Cas, Nucleic acid detection, Klebsiella pneumonia

Online: 11 November 2021 (12:57:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0189.v1

Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification

Lorena G. Caligiuri, Adolfo E. Sandoval, Jose C. Miranda, Felipe A. Pessoa, María S. Santini, Oscar D. Salomón, Nagila F. C. Secundino, Christina McCarthy

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sand fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia

Online: 17 December 2018 (10:41:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0364.v1

Papillary Thyroid Cancer Remodels the Genomic Fabrics of DNA Replication, Repair and Transcription

Dumitru A Iacobas, Sanda Iacobas

Subject: Biology And Life Sciences, Life Sciences Keywords: 8505C cells; BCPAP cells; ERCC#; evading apoptosis; PCNA; POLD4; POLR2L; TAF7; UBXN1

Online: 4 April 2024 (09:50:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0502.v1

WRKY22 Transcription Factor from Iris laevigata Regulates Flowering Time and Abiotic Stress Resistance

Lijuan Fan, Zhaoqian Niu, Guiling Liu, Gongfa Shi, Ziyi Song, Qianqian Yang, Sheng Zhou, Ling Wang

Subject: Biology And Life Sciences, Horticulture Keywords: Iris laevigata; WRKY transcription factor; flowering time regulation; salt stress; drought stress

Online: 9 October 2023 (11:38:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1276.v1

Understanding Transcription Factors and How They Affect Metabolic Processes in Cucumber Sex Determination

Szymon Turek, Aparna Aparna, Agnieszka Skarzyńska, Wojciech Pląder, Magdalena Pawełkowicz

Subject: Biology And Life Sciences, Plant Sciences Keywords: transcription factors; interactome network; sex development; sex determination; Cucumber (Cucumis sativus); metabolomic processes

Online: 1 May 2023 (00:01:26 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.1260.v1

Generation of Ultrashort Pulses in XUV and X-ray FELs via an Excessive Reverse Undulator Taper

Evgeny Schneidmiller, Matthias Dreimann, Marion Kuhlmann, Juliane Roensch-Schulenburg, Helmut Zacharias

Subject: Physical Sciences, Optics And Photonics Keywords: Free Electron Laser; SASE FEL; X-Ray FEL; Ultrashort pulses

Online: 30 April 2023 (04:45:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1144.v1

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Gabriel S.M. Sousa, Rodrigo S. De Oliveira, Alex B. De Souza, Ruan C. Monteiro, Elaine P. T. E. Santo, Luciano C. Franco Filho, Denison L.O. Moraes, Sarah R. De Sá, Silvia H. M. Da Silva

Subject: Biology And Life Sciences, Other Keywords: chromoblastomycosis; phaeohyphomycosis; PCR-Multiplex; Herpotrichiellaceae; sequencing; phenotypic; molecular biology.

Online: 19 March 2024 (12:35:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1348.v1

Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness

Pallavi Upadhyay, Jairus Reddy, Teddie Proctor, Oceane Sorel, Harita Veereshlingam, Manoj Gandhi, Vijay Singh

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Respiratory tract infections; COVID-19; influenza-like illness; multiplex PCR; syndromic panels

Online: 18 May 2023 (14:10:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0740.v1

Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow

Zachary P Morehouse, Caleb M Proctor, Gabriella L Ryan, Rodney J Nash

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Virus Detection; Viral Diagnostics; Diagnostics; SARS-CoV-2; Coronavirus; PCR

Online: 31 July 2020 (09:31:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0991.v1

Potential Performance of Two New RT-PCR and RT-q PCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes Allowing Efficient Entomological Surveillance of Both Vector-Borne Diseases

Michel Kiréopori Gomgnimbou, Louis robert wendyam BELEM, Etienne Bilgo, Miriam Félicité Amara, Zouera Laouali, Ali Ouari, Toussaint Bayala, Kobo Gnada, Raymond Kharlis Yao, Moussa Namountougou, Ibrahim Sangaré

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: multiplex rt-pcr; multiplex rt-qpcr; molecular detection; dengue virus; chikungunya virus; mosquitoes; Burkina Faso

Online: 12 July 2024 (08:43:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0236.v1

How to Shut Down Transcription in Archaea During Virus Infection

Simona Pilotto, Finn Werner

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Archaea; transcription inhibition; RNA polymerase; viruses; evolution; antibiotics

Online: 12 August 2022 (11:25:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0212.v1

Cell Stress Induces Mislocalization of Transcription Factors with Mitochondrial Enrichment

Chiara Rossi, Anna Fernàndez, Pascual Torres, Omar Ramirez-Nuñez, Ana Belén Granado-Serrano, Mònica Povedano, Reinald Pamplona, Isidro Ferrer, Manuel Portero-Otin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: TDP-43; Jun; REST; ERK; mitochondria; cell stress; aggregation; transcription factors; transgenic mice; subcellular fractionation

Online: 9 July 2021 (10:14:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0722.v1

Performance Analysis of Single Light Source Bidirectional Visible Light Communication Reverse Reflection Link

Ying Zhang, Jiawei Ren, Kexin Li, Haibo Mou

Subject: Engineering, Telecommunications Keywords: Visible light communications; uplink; moving retroreflective end; effective incidence angle

Online: 10 November 2023 (14:45:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.0120.v1

Amentoflavone and Nilotinib Show Strong Binding and Low Toxicity against HIV-1 Reverse Transcriptase: A Promising Antiretroviral Strategy

Ivan ferrari

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: HIV-1 reverse transcriptase; Pyrx program; Docking approach; Pro-Tox-II; pkCSM

Online: 3 September 2024 (11:25:25 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202111.0549.v1

PCR, PLS, or OPLS Evaluation of different regression techniques for hypothesis generation

Avani Ahuja

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Principal Component Regression, Partial Least Squares, Orthogonal Partial Least Squares, multivariate regression, hypothesis generation, Parkinson’s disease

Online: 29 November 2021 (15:42:03 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints201901.0298.v1

A Head and Neck Squamous Cell Carcinoma Model to Study and Target HPV Replication and Transcription

Michael R. Evans, Christian T. Fontan, Claire D. James, Xu Wang, Iain M. Morgan, Molly L. Bristol

Subject: Biology And Life Sciences, Virology Keywords: papillomaviruses; HPV; replication; transcription; E1; E2; IFIT1; cancer; HNSCC

Online: 30 January 2019 (04:42:41 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1410.v1

Oncogenic STAT Transcription Factors as Targets for Cancer Therapy: Innovative Strategies and Clinical Translation

Weiyuan Wang, Melanie Cristina Lopez McDonald, Rajashree Hariprasad, Tiara Hamilton, David A. Frank

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: STAT3; STAT5; cancer, immunity, cancer therapy

Online: 26 February 2024 (09:40:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1105.v1

Transcription Factor Regulation of Gene Expression Network by ZNF385D and HAND2 in Carotid Atherosclerosis

Ming Tan, Lars Juel Andersen, Niels Eske Bruun, Matias Greve Lindholm, Qihua Tan, Martin Snoer

Subject: Biology And Life Sciences, Life Sciences Keywords: Transcription factors; ZNF385D; HAND2; regulatory network; gene expression; carotid atheroma

Online: 15 January 2024 (07:51:47 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.0607.v1

From Transcription Factors to Personalized Vaccines: A Multifaceted Review of Glioblastoma Research and Treatment

Amol Tandon, Deepthi Nagalla

Subject: Biology And Life Sciences, Life Sciences Keywords: Glioblastoma; Transcription factors; Neoantigens; Personalized therapy; Epigenetics

Online: 10 October 2023 (09:55:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0227.v1

Analysis of Lin28B Promoter Activity and Screening of Related Transcription Factors in Dolang Sheep

Zhiyuan Sui, Yongjie Zhang, Zhishuai Zhang, Chenguang Wang, Xiaojun Li, Feng Xing, Mingxing Chu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Dolang sheep; Lin28B; promoter; transcription factor

Online: 12 April 2023 (02:48:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202301.0215.v1

The Role of the Fusarium oxysporum FTF2 Transcription Factor in Host Colonization and Virulence

Virginia Casado-del Castillo, Ernesto P. Benito, José María Díaz-Mínguez.

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Fusarium oxysporum; virulence; FTF gene family; plant colonization; effector

Online: 12 January 2023 (07:31:36 CET)

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Preprint REVIEW | doi:10.20944/preprints202006.0277.v2

NKX6.1 Transcription Factor: A Crucial Regulator of Pancreatic β-Cell Development, Identity, and Proliferation

Idil Aigha, Essam Abdelalim

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Diabetes; transcription factor; β-cell mass; pluripotent stem cells; pancreatic progenitors; cell therapy

Online: 19 October 2020 (16:04:27 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202306.2007.v1

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega, Klaudia Babisova, Michaela Hyblova, Nikola Janostiakova, Peter Sabaka, Pavol Janega, Gabriel Minárik

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Online: 29 June 2023 (02:12:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint REVIEW | doi:10.20944/preprints202301.0461.v1

Transcription Factors and Intracellular Proteins Orchestrate Phenotypic Adaptation in Cryptococcus neoformans for Survival and Human Infections

Olufemi Samuel Folorunso, Olihile Moses Sebolai

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Cryptococcus; transcription factor; thermotolerance; virulence factors; antifungal; oxidative and osmotic stress; capsule; melanin; monokaryotic fruiting; mating and filamentation

Online: 26 January 2023 (02:54:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0138.v1

Global Analysis of the WOX Transcription Factor Family in Akebia trifoliata

Shengpeng Chen, Huai Yang, Yongle Zhang, Chen Chen, Tianheng Ren, Feiquan Tan, Peigao Luo

Subject: Biology And Life Sciences, Plant Sciences Keywords: Akebia trifoliata; WUSCHEL-related homeobox; transcription factor; adventitious roots.

Online: 2 November 2023 (09:11:33 CET)

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