The real systems are often complex, nonlinear, and noisy in various areas including mathematics, natural science, and social science. We present the symplectic entropy (SymEn) measure as well as an analysis method based on SymEn to estimate the nonlinearity of the complex system by analyzing the given time series. The SymEn estimation is a kind of entropy based on symplectic principal component analysis (SPCA) which represent organized but unpredictable behaviors of systems. The key to SPCA is to preserve the global submanifold geometrical properties of the systems through symplectic transform in the phase space, which is a kind of the measure-preserving transforms. The capability of preserving the global geometrical characteristics makes the SymEn a test statistic to detect the nonlinear characteristics in several typical chaotic time series and the stochastic characteristic in the Gaussian white noise. The results are in agreement with findings in the approximate entropy (ApEn), the sample entropy (SampEn) and the fuzzy entropy (FuzzyEn). Moreover, the SymEn method is also used to analyze the nonlinearities of the real signals (including the EEG signals for ASD and healthy subjects, and the sound and vibration signals for the mechanical systems). The results indicate that the SymEn estimation can be taken as a measure for describing the nonlinear characteristics in the data collected from the natural complex systems.

Silty clay modified by fly ash and crumb rubber is a kind of sustainable subgrade filler which has good anti-freeze-thaw resistance stability but wake vibration isolation performance. The objective of this study was to improve the vibration isolation of the modified soil and investigate the vibration isolation effect of the composite subgrade structure of XPS plates and the modified soil by indoor impact test. First, the vibration isolation performance of silty clay, modified soil and composite subgrade structure was respectively evaluated. Second, the effect of XPS plate’s thickness and vibration intensity on the vibration performance of the composite subgrade structure were evaluated. Third, the vibration isolation performance of the test groups under the condition of freeze-thaw cycles was assessed. The results show that the vibration isolation performance of subgrade can be effectively improved by setting XPS plates. The composite subgrade structure has certain vibration isolation effect, especially in vertical direction. Considering vibration isolation performance and costs, 5cm is the optimum XPS plates thickness. The composite subgrade structure has great vibration isolation performance under the condition of freeze-thaw cycles, so it is suitable to be applied in road subgrade in seasonal frozen regions.

Magnetic levitation vibration isolation platform are essential for testing space telescopes. This paper presents the design of a Large Air-Gap Voice Coil Motor (LAG-VCM) specifically for use in such platforms. The electromagnetic design of the voice coil motor begins with the Equivalent Magnetic Circuit Method, followed by the analysis and optimization of the motor design using Finite Element Analysis (FEA). Subsequently, a prototype was constructed and tested. The test results confirm that the prototype meets the design specifications and requirements.

In this paper, based on the cumulated damage generated by the cyclical application of the testing vibration profile, a methodology to determine the reliability index of the analyzed component is given. In the analysis, the testing time, component geometry, applied weight, and material resonant frequency are all of them considered by using a dynamic, and amplitude factor. Then, from the generated vibration response data of the analysis, the corresponding bending stress is determined for each one of the rows of the testing profile. And by the cyclical application of the whole testing profile, the corresponding damage is cumulated to each profile cycle until the cumulated damage is D=1. Finally, the reliability that each one of the cumulated blocks (cyclical profile application) is determined by considering, the random vibration is a stress variable. Thus, we use the cumulative damage model with the inverse power law stress/life relationship to determine the two parameter Weibull distribution used to estimate the reliability index of each cumulated block. As a numerical application, we analyze a cantilever aluminum beam Al 6061-T6.

Keywords: Linear differential arrays; beam steering; frequency-invariant beamforming.

Vibration signal analysis is regarded as a fundamental approach in diagnosing faults in rolling bearings, and recent advancements have shown notable progress in this domain. However, the presence of substantial background noise often results in the masking of these fault signals, posing a significant challenge for researchers. In response, an Adaptive Denoising Autoencoder (ADAE) approach is proposed in this paper. The data representations are learned by the encoder through convolutional layers, while the data reconstruction is performed by the decoder using deconvolutional layers. Both the encoder and decoder incorporate adaptive shrinkage units to simulate denoising functions, effectively removing interfering information while preserving sensitive fault features. Additionally, dropout regularization is applied to sparsify the network and prevent overfitting, thereby enhancing the overall expressive power of the model. To further enhance ADAE's noise resistance, shortcut connections are added. Evaluation using publicly available datasets under scenarios with known and unknown noise demonstrates that ADAE effectively enhances the signal-to-noise ratio in strongly noisy backgrounds, facilitating accurate diagnosis of faults in rolling bearings.

Sunflower is one of the oil crops and its planting areas was listed behind Soybean, peanut and rapeseeds in China. It will encounter different kind of diseases during planting, thus limited the seeds yield. Fusarium species are the major threat to different crops nowadays, it is very important to identify the species caused diseases on different hosts, so that the potential control measures may be explored. In Inner mongolia, Sunflower Disk Rot (SDR) was found in different sunflower planting field, the average infection ratio around 8.50%, typically in some verieties, its infection ratio reach to 11.67%. The diseased receptacle were collected from different locations and Koch’s postulate was conducted. The results showed that Fusarium verticillioides was the main causal agent caused SDR. Fungicide toxicity tests shown that Fludioxonil and Flutolanil were screened out from six fungicides for controlling F. verticillioides, with EC50 values of 0.05 µg/mL (R = 0.9825) and 0.96 µg/mL (R = 0.9964) , respectively. This is the first report of SDR caused by F. verticillioides, and it will alert sunflower reseachers to include SDR in the disease list, so as to control sunflower diseases with integrated management strategy successfully.

The dynamic behaviour of a machine tool (MT) directly influences the machining performances. The adoption of lightweight structures may reduce the effects of undesired vibrations and increasing the workpiece quality. This paper aims to present and compare a set of hybrid materials that may be excellent candidate to fabricate the MT moving parts. The selected materials have high dynamic characteristics and capacity to damp mechanical vibrations. In this way, starting by the kinematic model of a milling machine that highlights the main critical factors, this study paper evaluates a number of prototypes made of Al Foam sandwiches (AFS), Al Corrugated sandwiches (ACS) and materials reinforced by carbon fibres (CFRP). A set of prototypes has been fabricated, represented the Z-axis ram of a commercial milling machine. The static and dynamical properties have been evaluated by using both FE simulations and experimental tests. The obtained results show that the proposed structures may be a valid alternative to the conventional materials of MT moving parts, increasing machining performances. In particular, the AFS prototype highlighted a damping coefficient that is 20 times greater than a conventional ram (e.g. steel). The CFRP structure is able to satisfy the machining requirements with a reduced weight of 48.5%, while the ACS prototype showed a good trade-off between stiffness and damping.

This work discusses seismic isolation technologies and analyses the concept of geotechnical seismic isolation (GSI) system, which is characterized by new principles of operation, using geomaterial with modified properties to reduce inertial seismic loads on buildings and structures. The aim of the study is to develop a geotechnical seismic isolation system in the form of vertical barriers, with different geometrical characteristics, using rubber-soil mixture (RSM). The novelty of the work consists in determination of effective constructive and technical solutions of vertical seismic barriers from RSM, which are characterized by reliability in providing seismic isolation of construction objects. Soil and superstructure interactions are modeled in Plaxis software, using the Northridge earthquake accelerogram. The results confirm the positive impact of using RSM as an effective material for GSI vertical barriers. The present numerical and experimental results show that the GSI system using RSM can significantly reduce the seismic hazard. The results of computational-experimental modeling will become a research, analytical and design basis for the development of new ways to ensure earthquake resistance of construction objects.

The performance of the absolute atom gravimeters used on moving platforms, such as vehicles, ships and aircrafts, is strongly affected by the vibration noise. To suppress its influence, we summarize a vibration compensation method utilizing data measured by a classical accelerometer. The measurements with the accelerometer show that the vibration noise in the vehicle can be 2 order of magnitude greater than that in the lab during daytime, and can induce an interferometric phase fluctuation with a standard deviation of 16.70π. With the compensation method, our vehicle-mounted atom gravimeter can work normally in these harsh conditions. Comparing the Allan standard deviations before and after the vibration noise correction, we find a suppression factor of 22.74 can be achieved in static condition with an interrogation time of T = 20 ms, resulting a sensitivity of 1.35 mGal/Hz1/2, and a standard deviation of 0.5 mGal with an average time of 10 s. We also demonstrate the first test of an atom gravimeter in a moving vehicle, in which a suppression factor of 50.85 and a sensitivity of 60.88 mGal/Hz1/2 were realized with T = 5 ms.

In this paper, the mechanism of combined process of ion beam sputtering (IBS) and HF acid etching on the chemical structure defects of fused silica and its laser damage resistance perfor-mance were investigated. During the removal process of surface material, the sputtering effect causes lattice atoms to detach from their original positions, forming a large number of unsaturated structures on silica surface, which improves the chemical activity of Si and O atoms, accelerates the chemical reaction process between surface atoms and water molecules, increases the content of hydroxyl (OH-) groups in the shallow layer, and enhances the photothermal weak absorption intensity. However, the presence of OH- groups reduces the bonding strength of Si-O, destroys the spatial network structure of silica bulk, and reduces the mechanical strength of silica surface, resulting in a decrease in its laser damage resistance performance. The paper reveals for the first time the mechanism of IBS to change the structure characteristics of silica material, accelerate the surface hydroxylation process, and thereby reduces the laser damage resistance performance. This work provides technical guidance for effectively suppressing chemical structure defects on silica surface and improving laser damage resistance performance of optical components under high-flux laser irradiation.

Vibration problems have become one of the most important factors affecting robot performance. To this end, a terminal residual vibration suppression method based on joint trajectory optimization is proposed to improve the accuracy and stability of robot motion. Firstly, based on the characteristics of the friction nonlinearity due to joint coupling and physical feasibility of dynamic parameters, a semidefinite programming method is used to identify dynamic parameters with actual physical meaning, thereby obtaining an accurate dynamic model. Then, based on the result of the residual vibration time domain analysis, a joint trajectory optimization model with the goal of minimizing joint tracking error is established. The Chebyshev collocation method is used to discretize the optimization model. The dynamic model is used as the optimization constraint, and barycentric interpolation is used to obtain the optimized joint motion trajectory. Finally, industrial robot experiments prove that the vibration suppression method proposed in this article can reduce the maximum acceleration amplitude of residual vibration by 62% and the vibration duration by 71%. Compared with the input shaping method, the method proposed in this paper can reduce the terminal residual vibration more effectively and ensure the consistency of running time and trajectory.

Focal vibration therapy can provide neurophysiological benefits. Unfortunately, standardized protocols are non-existent. Previous research presents a wide range of protocols with a wide range of effectiveness. This paper is part of a broader effort to identify effective, standardized protocols for focal vibration therapy. The vibration characteristics of four commercially available focal vibration devices that have been used for research and clinically were measured. An accelerometer was used for the measurements. Frequency and peak-to-peak amplitude were measured. Measurements were made when the devices were free and then again when they were strapped to the human body. Vibration frequency ranged from 120 to 225 Hz. Free vibration amplitude ranged from 2.0 to 7.9 g’s (peak-to-peak). When the devices were strapped to the body (constrained), vibration amplitude decreased by up to 65.7%. These results identify effective ranges of focal vibration frequency and amplitude. They illustrate the importance of identifying vibration environment, free or constrained, when quoting vibration characteristics. Finally, the inconsistency of multi-actuator devices is discussed. These results will guide protocol development for focal vibration and potentially better focal vibration devices.

This paper proposes an innovative mechatronic piezo-actuated module to control vibrations in modern machine tools. Vibrations represent one of the main issues that compromise seriously the quality of the workpiece. The active vibration control (AVC) device is composed by a host part integrated with sensors and actuators synchronized by a regulator, able to make a self-assessment and adjust to the environmental alteration. This study presents the mechatronic model based on the kinematic and dynamic analysis of the AVC device. To ensure a real time performance, a H2-LQG controller has been developed and validated by simulations involving machine tool, PZT actuator and controller models. The Hardware-in-the-loop (HIL) architecture is adopted to control and attenuate the vibrations. A set of experimental tests has been performed to validate the AVC module on a commercial machine tool. The feasibility of the real time vibration damping is demonstrated and the simulation accuracy is evaluated.

Loneliness is becoming prevalent across Japan in spite of its a dense population and communal culture. Surveys show 40% of adults report isolation across demographics. The contradiction emerges due to deteriorating social structure with modernization, economic parity, urbanization, and fading conventional setup. The continually social withdrawals, called “hikikomori." Relief seekers have embraced AI based emotive robots, digital avatars, and immersive VR as low-stakes social surrogates despite limited capacity to cultivate human intimacy. To stabilise government attempts awareness through reforms, scheme and support services. But a concrete transformation can be brough in with work-life balance, gender equality and rewire the metrics for self-esteem. Even a perfectly personalized AI cannot replace the human affection lacking in the society. Lasting solutions demand technological aids be balanced by confronting and reinventing community purpose and bring in structural change. Overall Japan’s endemic isolation reveals the societal fraying when modernization privileges independence over interdependence. The path forward rests on restoring cultural foundations to reverse loneliness and social isolation.

Several bio-actors are involved in the occurrence of leishmaniasis, which is made too complex for prevention and control options. Currently, all forms of leishmaniasis are being treated with chemical drugs, which have limitations and adverse effects on patients. Searching the bioactive molecules from plant sources allows the development of a novel drug. The essential oil and solvent extract of the roots of E. kebericho showed antileishmanial activity. But looking for biological activities of the isolated active ingredient from tubers of E. kebericho was scant. Thus, the isolation of the leishmanicidal compound from the roots of E. kebericho through a bioassay-guided technique was an aim of this work. Dehydrocostus lactone showed doubled potent than the standard drug miltefosine on leishmanicidal activities against L. major and L. tropica. Besides, the broad-spectrum biological activities of dehydrocostus lactone could be a potential drug candidate for co-infected patients caused by an association of leishmania and another pathogen.

Sea cucumber peptides are small molecular peptides with biological function obtained from fresh sea cucumber as feedstock, which were hydrolyzed by protease, separated ,and purified. As the main product of sea cucumber refinement, it more accurately expresses the nutritional effect of sea cucumber and has a wide range of potential applications. It has been demonstrated that sea cucumber has a certain value-added effect on bone mineral density regulation, but the differences in bone mineral density regulation of extracts from different parts of sea cucumber and the techniques contributing to the efficient isolation of bone sea cucumber peptides have not been reported. Firstly, the aim of this study was to reveal the differences in osteogenic activities of enzymatic peptides from three major parts of sea cucumber: the body wall, the egg protein, and the intestinal protein. Then, the bone-enhancing sea cucumber peptides were isolated based on the characteristics of the dif-ferences in molecular weight distribution, hydrophilicity, and ionisation degree of sea cucumber, and to explore the possible mechanisms of their action. The findings indicated that sea cucumber peptides extracted from three distinct sites exhibited a dose-dependent enhancement of osteoblast proliferation and differentiation. Upon evaluating the three methods of sea cucumber peptide separation based on molecular weight distribution, hydrophilicity, and ionization, it was observed that the fraction isolated by hydrophobicity and

Quite a number of cottonseed varieties are existed. The seeds of each variety produce a different amount of gossypol as a natural toxic compound. The increased interest in this natural product and its toxic effects were enhanced the demand for establishing simple silica-gel-based methods for its isolation, purification, and characterization. Historically, silica gel plays a significant role in isolation and purification of many different natural products. Silica gel is variants and can intro-duce a lab, simple tool for tracking a compound in certain plants during its purification process. In this study, gossypol was isolated and purified in the form of yellow crystals as gossypol acetic ac-id. Several methods and tools were used for its characterization including TLC, preparative TLC, silica gel column, UV/IR spectrophotometer and HPLC (Robust spherical silica gel). A compara-tive study between its amounts in both Egyptian and Chinese varieties were performed. Under the experimental condition, the Egyptian cottonseeds contain 8.705 gm/kg, while the Chinese contains 5.395 gm/kg. Thin Layer Chromatography (TLC) had been flourished the science of the phytochemistry and still popular till nowadays and is recommended for fast, accurate, and inex-pensive evaluation and quantification of gossypol. It is recommended to isolate and purify gossy-pol as gossypol acetic acid using optimized combined methods used in this study. Using Silica gel different protocols will assist and facilitate the parallel comparison and save the compact column from any unexpected contaminants during the purification steps. During each step the silica gel itself could be simply removed by paper filtration.

The increase in the frequency of droughts in Africa has increased the interest in crops with drought escape mechanisms like grain Amaranth (Amaranthus hypochondriacus). However, the white-seeded grain Amaranth is easily cross-pollinated by its black seeded wild relatives such as smooth pigweed (Amaranthus hybridus). A study to investigate a minimum isolation distance enough to prevent seed contamination study was carried out at three sites namely Gweru and Bindura (2020/2021 rainy season) and Shamva (2021/2022 rainy season). Smooth pigweed was planted at distances of 3 to 150 m from pure grain Amaranth. Close monitoring of phenology and measurement of meteorological conditions such as temperature, wind speed and direction were done during the anthesis period. Cross-pollination in grain Amaranth was influenced by distance from the pollen source. A maximum of 12.9 % contamination rate was measured within the 10 m radius from the pollen donor plot and a minimum outcrossing rate of 0.45 % was recorded at the widest distance of 150 m. It can be concluded that complete removal of wild relatives of grain Amaranth is important within the field at as far as 150 m from the crop.

(1) Background: In most cases, passive isolation control methods are commonly used for the seismic isolation design of bridge engineering. However, for passive seismic isolation devices, due to their non-adjustable performance parameters, it is challenging to achieve a good seismic isolation effect for structures under random and variable seismic loads in the wide frequency range of 0 Hz to 20 Hz; (2) Methods: the sensitivity of the design parameters of the seismic isolation bearing was analyzed using the optimization center gradient method, and an improved genetic algorithm was employed to quickly optimize and obtain the optimal design parameters; (3) Results: the effectiveness of the three-dimensional seismic isolation bearing was validated through experiments. (4) Conclusions: The multi-factor sensitivity analysis approach used in this study for designing novel isolation bearings is applicable not only to seismic design in bridges but also serves as a reference for parameter design in isolation bearings requiring medium to high precision in seismic performance.

Roof and roofings built to protect the housing and homeowner from undesirable rainwater, have been seen an important structural element throughout history in the entire of housing. In this research, structural and spatial relationships between building envelope with isolation from water in traditional housing architecture are analyzed. Roof, eaves and rain gutters which are isolation from water items were investigated according to shapes, slope direction, materials, carrier systems and covering materials. The qualification of the roofing, eave and rain gutter elements which are equipment elements related to the comfort of the building envelope and the extent of changes in these elements were examined at the traditional housing level. Exemplary, the traditional housing in the city of Trabzon in Turkey were selected. During the completion process of the working, detailed aims related to structure-health reinforcements were specified with the relevant literature review. The chart form has been developed for compilation of information about the general characteristics of the housing in the research area and the building-health reinforcements within the aim of the research. Exemplary houses were selected by external observation technique and the data obtained as a result of observations, interview and determinations related to general characteristics and building-health reinforcements of these housing were handled into a chart form. By evaluating the data obtained; harmony and contrasts in system level practices, the dimensional properties of the elements at the spatial level, the greatness and accuracy levels preferred in various fittings, the harmony and contrasts in the materials, dimensions and practices of the elements at the system and spatial level, harmony and contrasts of applications of new opportunities at the level of building-health reinforcements in traditional housing, conclusions were drawn to identify special and general situations at the level of building-health reinforcements and to obtain information about the comfort requirements of traditional housing. Nowadays, where the health of the building, which is mostly ignored, is gaining importance day by day, concrete and applicable solution data are presented and interpreted for new designs to be made in this direction.

Silicon carbide particle-reinforced aluminum matrix (SiCp/Al) composites are significant lightweight metal matrix composites extensively utilized in precision instruments and aerospace sectors. Nevertheless, the inclusion of rigid SiC particles exacerbates tool wear in mechanical machining, resulting in a decline in the quality of surface finish. This work undertakes a comprehensive investigation into the problem of tool wear in SiCp/Al composite materials throughout the machining process. Initially, a comprehensive investigation was conducted to analyze the effects of cutting velocity vc, feed per tooth fz, milling depth ap, and milling width ae on tool wear during high-speed milling under SCCO2-MQL (Supercritical Carbon Dioxide Minimum Quantity Lubrication) ultrasonic vibration conditions. Furthermore, a prediction model for tool wear was developed by employing the orthogonal test method and multiple linear regression. The model's relevance and accuracy were confirmed using F-tests and t-tests. Ultimately, through the utilization of a genetic algorithm, the milling parameters were optimized, resulting in the identification of the most optimal combination of parameters. Empirical findings suggest that the careful selection of milling parameters can significantly mitigate tool wear, extend the lifespan of the tool, and enhance the quality of the surface. This work serves as a significant point of reference for the processing of SiCp/Al composite materials.

Background: Breast cancer and other neoplasms, such as acute lymphocytic leukaemia, are a severe hazard to public health globally. L-glutamine-degrading enzymes are required for the treatment of malignancies such as acute lymphocytic leukemia and breast cancer. L-glutaminase, an amidohydrolase, has been shown to be an effective chemotherapeutic tool for treating a variety of malignancies. The aim of the study: Molecular characterization is used in the study and production of bacterial L- glutaminase as an anticancer agent from a variety of Egyptian soil locales. The type of the study: Screening experimental study. Methodology: In the current study, many bacterial isolates were tested on mineral L-glutamine agar selective medium (MGA) to evaluate if they generated L-glutaminase. The most effective positive bacterial isolates that produce L-glutaminase, however, were discovered by morphological and biochemical analysis. The primary positive isolate producing L-glutaminase was also identified utilizing molecular detection by 16S rRNA sequencing homology technique. The properties of bacteria-produced L- glutaminase were investigated. The anticancer activity was assessed with an MTT assay. Results: Sole bacterial isolates that only needed L-glutamine for metabolic nitrogen grew well on MGA. The optimal conditions for producing positive bacterial isolates were pH 7.4 and 37 ̊C. The morphological and biochemical investigations of soil samples from diverse soil conditions in Egypt revealed that Bacillus subtilis subsp. niger (ATCC 9372) was the primary positive bacterial isolate producing L-glutaminase. At a pH of 7.4 and a temperature of 37 °C, the activators KCL, ZnSO4, FeSO4, K2HPO4, and MgSO4 offered ideal conditions for defining L-glutaminase production. L-glutaminase from Bacillus subtilis was detected to be a highly bioavailable and efficient anticancer treatment. The original yield [productivity] from MGA was 6.3 U/ml, but invitro coupled transcription and translation production raised it to 89± 1 U/ml. L-glutaminase had a molecular mass of approximately 36 kDa. The purification of L-glutaminase resulted total activity of 21,883 ± 15.67 (U), specific activity of 379.14 ± 7.35 (U/mg of protein), and purification fold of 1.82 ± 3.06. The enzymatic activators Ni2+ and Mg 2+ increased L-glutaminase activity by 20%, 27%, respectively. The compound showed significant anticancer activity (IC50 = 38.72, 8.6, 6.41, 19.02, 49.83, and 9.45 μg/ml) against cancer cell lines from the liver (HepG-2), colon (HCT- 116), breast (MCF-7), lung (A-549), lymphocytic (CCL-120), and cervical (Hela). The kinetic parameters Km and Vmax were 15.2^ 10 -3 M and 119.86 μmol/ml/min, indicating a higher affinity for the substrate. The enzyme demonstrated high efficacy at pH 6.5 and temperature 35. The enzyme was thermostable at 58 ̊C for 1 hour. Conclusion: Acute lymphocytic leukemia and breast cancer are two instances of auxotrophic malignancies for which L-glutamine is the sole metabolic source, and Bacillus subtilis- derived L-glutaminase was a suitable enzyme in their treatment.

To make wind power more competitive, it is necessary to reduce turbine downtime and reduce costs associated with wind turbine Operation and Maintenance (O&M). Incorporating machine learning in developing condition-based predictive maintenance methodologies for wind turbines can enhance their efficiency and reliability. This paper presents a monitoring method that utilizes Base Density for the Support Vector Machine (BDSVM) and the evolutionary Fourier spectra of vibrations. This method allows smart monitoring of the function evolution of the turbine. A complex optimal function (FO) for 5-degree order has been developed that will be the boundary function of the BDSVM to timely determine the magnitude, frequency, and place of the failure occurring in wind turbine drive.

Early detection of neurological conditions is critical for timely diagnosis and treatment. Identifying cellular-level changes is essential for implementing therapeutic interventions prior to symptomatic disease onset. However, monitoring brain tissue directly through biopsies is invasive and high-risk. Bodily fluids such as blood or cerebrospinal fluid contain information in many forms, including proteins and nucleic acids. Particularly, cell-free DNA (cfDNA) has potential as a versatile neurological biomarker. Yet, our knowledge of cfDNA released by brain tissue and how cfDNA changes in response to deleterious events within the brain is incomplete. Mapping changes in cfDNA to specific cellular events is difficult in vivo, where many tissues contribute to circulating cfDNA. Organoids are tractable systems for examining specific changes consistently in a human background. However, few studies have investigated cfDNA released from organoids. Here, we examined cfDNA isolated from cerebral organoids. We found that cerebral organoids release quantities of cfDNA sufficient for downstream analysis with droplet-digital PCR and whole-genome sequencing. Further, gene ontology analysis of genes aligning with sequenced cfDNA fragments revealed association with terms related to neurodevelopment and autism spectrum disorder. We conclude that cerebral organoids hold promise as tools for the discovery of cfDNA biomarkers related to neurodevelopmental and neurological disorders.

In this paper the possibility of harvesting energy from the vibrations of a plate is analyzed. The harvester takes the form of a cantilever dynamic vibration absorber equipped with a piezoelectric layer and tuned by means of a tip mass to the first mode of vibration of the plate. A mathematical model of the coupled system composed of the plate and the harvester is presented. The validity of the proposed harvester is proved by means of simulations carried out with the modal expansions approach. Simulation results highlighting the effect of harvester tuning and location are presented as well. Then the validity of the harvester is confirmed by experimental tests carried out both with a concentrated impulsive load and with a distributed pressure load. Simulations and experimental tests show that the cantilever piezoelectric dynamic vibration absorber generates open circuit voltage an order of magnitude larger than the one generated by the same piezoelectric layer when directly bonded to the plate surface.

The vibration response of soil is a key property in the field of agricultural soil tillage. Vibration components of tillage machinery are generally used to reduce tillage resistance and improve work efficiency, the pressure variation under low-frequency vibration will affect the fragmentation and dispersion of farmland soil. However, the gradient of pressure variation, frequency domain response, and effective transmission range are unclear. A new method based on DEM (Discrete Element Method) is presented to study the vibration response and pressure transmission under low-frequency vibration. Bench test results showed that peak pressure positively correlates with the vibration frequency and attenuates rapidly at the vibration distance of 100 to 250 mm. The results data were also selected to determine the simulation model parameters. Amplitude, vibration frequency, and soil depth were used as test factors in single-factor simulation tests, and their effects on the peak pressure, frequency domain response, and effective transmission distance were analyzed. The results showed a positive relationship between the peak pressure and the test factors. The peak pressure increases with a maximum gradient of 19.02 kPa/mm at a vibration distance of 50 mm. The amplitude, vibration frequency, and soil depth positively correlate with the dominant frequency amplitude. The main frequency is independent of amplitude and soil depth. At the vibration distance of 250 mm, the dominant frequency is approximately twice the vibration frequency at 7–11 Hz and approximately equal to the vibration frequency at 13–15 Hz. Multiple exponential functions were used to fit the peak pressure attenuation function, obtaining an effective transmission distance range of 347.15 to 550.37 mm for the 5kPa cut-off pressure. For a soil depth of 300 mm, the vertical shear wave diffusion angle is greater than the horizontal shear wave diffusion angle. The study clarifies the vibration response of soil under low-frequency vibration, which helps to design vibration type soil-engaging components of tillage machinery and match vibration parameters for energy saving and resistance reduction in soil tillage.

Vibration measurement serves as the basis for various engineering practices such as natural frequency or resonant frequency estimation. As image acquisition devices become cheaper and faster, vibration measurement and frequency estimation through image sequence analysis continue to receive increasing attention. In the conventional photogrammetry and optical methods of frequency measurement, vibration signals are first extracted before implementing the vibration frequency analysis algorithm. In this work, we demonstrated that frequency prediction can be achieved using a single feed-forward convolutional neural network. The proposed method is verified using a vibration signal generator and excitation system, and the result obtained was compared with that of an industrial contact vibrometer in a real application. Our experimental results demonstrate that the proposed method can achieve acceptable prediction accuracy even in unfavorable field conditions.

Consumption of refined foods and beverages high in sugar make the teeth susceptible to the formation of biofilm, and lead to dental caries and diseases of the oral cavity such as periodontitis and periodontal disease. The aim of the present study was to determine the ability of selected probiotics to inhibit growth and biofilm formation by the cariogenic bacterium Streptococcus mutans in vitro. We screened strains of latic acid bacteria (LAB) (n=120) from the Bioresources Collection and Research Center (BCRC), saliva of healthy adults and infant stool. The antimicrobial activity of LAB in vitro was evaluated by agar spot culture and co-culture of the S. mutans strains. We determined the effect of heating and dilution factors (2- and 4-fold) on the antagonistic activity of LAB. Antagonistic substances in the spent culture suspensions (SCS) of LAB were precipitated by extraction with ammonium sulphate and chloroform to characterise the protein and lipophilic fractions. Results of co-culturing show that the SCS of the three LAB strains (Lactobacillus pentosus 13-1, 13-4 and Lactobacillus crispatus BCRC 14618) subjected to heat treatment showed significantly high antimicrobial activity. We found that substances produced by L. pentosus 13-4 which have the potential to exhibit antimicrobial properties might be lipophilic proteins. Additionally, we infer that the mechanism of reducing biofilm formation by Lactobacillus strains is associated with sucrose-dependent cell–cell adhesion and the gtfC level of glucosyltransferases (Gtfs) in the biofilm. Native LAB strains screened in our study may be used in chewing gums and other processed foods for preventing tooth decay.

In contemporary construction, the prevalence of vibration serviceability issues in lightweight and slender structures has become increasingly common, owing to advancements in building materials and construction methods. While these structures often meet the criteria for ultimate limit states, they can still elicit complaints due to excessive vibrations induced by human activity. To address this challenge, the Integral Resonant Control (IRC) technique has emerged as a favored approach for actively damping vibrations in various systems. This study introduces a fresh perspective on implementing a multi-input multi-output (MIMO) IRC scheme for active vibration control (AVC) specifically tailored for pedestrian structures utilizing inertial mass actuators. Building upon a common framework and design methodology outlined in previous research, this work presents a novel application of MIMO IRC for AVC. The designed controller is rigorously tested and implemented on a laboratory floor structure to validate its efficacy.

Vibration monitoring is a critical aspect of assessing the health and performance of machinery and industrial processes. This study explores the application of machine learning techniques, specifically the Random Forest (RF) classification model, to predict and classify chatter—a detrimental self-excited vibration phenomenon—during machining operations. While sophisticated methods have been employed to address chatter, this research investigates the efficacy of a simplified RF model. The study leverages simulated vibration data, bypassing resource-intensive real-world data collection, to develop a versatile chatter detection model applicable across diverse machining configurations. The feature extraction process combines time-series features and Fast Fourier Transform (FFT) data features, streamlining the model while addressing challenges posed by feature selection. By focusing on the RF model’s simplicity and efficiency, this research advances chatter detection techniques, offering a practical tool with improved generalizability, computational efficiency, and ease of interpretation. The study demonstrates that innovation can reside in simplicity, opening avenues for wider applicability and accelerated progress in the machining industry.

Context: The global escalation of conflict, violence, and human rights violations sets a pressing backdrop for examining the resilience of forcibly displaced youth (FDY) in Canada. This study aims to unpack the multifaceted challenges and resilience mechanisms of FDY, focusing on their health, well-being, and integration into host communities. It seeks to identify current models of resilience, understand the factors within each model, and highlight gaps and limitations. Methodology: Using a university librarian-supported structured search strategy, this exploratory rapid review searched literature from Ovid Medline and Open Source databases, published in English between January 2019 and January 2024, that fit specific inclusion criteria. The eligible articles (N=12 out of 4,096) were charted and analyzed by two student researchers with the PI. Charted data were analyzed thematically. Results: The selected studies captured diverse geographical perspectives, resilience models (such as Ungar's ecological perspective and Masten's resilience developmental models), as well as protective and promotive frameworks. Key findings indicate the complexity of resilience influenced by individual, familial, societal, and cultural factors. Each model offers insights into the dynamic interplay of these influences on FDY's resilience. However, these models often fall short of addressing the nuances of cultural specificity, the impact of trauma, and the intersectionality of FDY's identities. Conclusion: Recognizing the diverse and evolving nature of FDY's coping mechanisms, this study advocates for a culturally appropriate approach to resilience that integrates an intersectionality framework of individual attributes and culturally sensitive models.

Zucchini (Cucurbita pepo L.) is an important crash crop for producing seeds in Inner Mongolia, China. In August 2021, the harvest season of seed-producing zucchini, fruit rot symptom (cv. Jindi No.1) was found in the Wuyuan region. The incidence of infected fruits of zucchini ranged from 10% to 30% and symptom consisted in 4 to 5 cm diameter lesions where the fruit was in contact with the soil. Ten isolations from six symptomatic fruits were preliminary classified as Fusarium species based on its morphological characters in potato dextrose agar. Phylogenetic tree was constructed by concatenating TEF-1α and RPB2 gene and exhibited the isolates clustered together with the Fusarium solani f. sp. cucurbitae sequences. Pathogenicity tests revealed that two isolates caused over 3 cm diameter lesions on the fruits while negative-controls remained asymptomatic. The colonies reisolated from the leasions were morphologically identical to the original isolates, thus fulfilling Koch’s postulates. Results demonstrated that F. solani f. sp. cucurbitae was the causal agent of fruit rot observed in the field. To our knowledge, this is the first report of fruit rot of seed-producing zucchini caused by F. solani f. sp. cucurbitae in Inner Mongolia, China.

This paper addresses the analysis for the nonlinear vibration response of a rectangular tube with a flexible end and non-rigid acoustic boundaries. This is a further work of the linear structural acoustic problem in a well-known acoustic book. In fact, the acoustic boundaries of an enclosed space sometimes are non-rigid and the structural vibration responses are nonlinear. These two points are the focuses of this paper. The multi-level residue harmonic balance method is applied to this nonlinear structural acoustic problem. The results obtained from the multi-level residue harmonic balance method and numerical method are generally in good agreement. The effects of excitation magnitude, tube length, and phase shift parameter etc. are examined.

A problem of efficacy of crystal growth methods for crystallization from solution or melt has been investigated. Axial vibrational control (AVC) technique was considered as a perspective method to manage both heat-mass transfer and chemical component composition of the melts in the case of crystallization of complex chemical compounds. Numerical modeling and the search for generalized dependencies made it possible to predict the AVC parameters that provide optimal heat and mass transfer modes for creating flat liquid-solid interfaces, as well as the component composition of dissociated melts of various chemical compounds – Ge, NaNO3, CdTe.

This study analyzes the modeling and dynamics of a novel passive in Multi-Degree-of-Freedom (MDOF) vibration isolation platform which can achieve significant isolation effect. Symmetrical Scissor-Like structures (SLSs) are utilized in the proposed MDOF isolation platform as the supporting and isolation elastic components. Based on the mathematical modeling and theoretical analysis of the MDOF vibration isolation system with SLSs, the effect of structural parameter and joint friction on stiffness and damping properties is investigated. It is shown that due to geometric relations within the SLSs, the natural frequencies can be reduced via adjusting structural parameters of the SLS for different direction vibration isolation. Theoretical and experimental results show that the SLS isolation platform can achieve much better loading capacity and vibration isolation performance simultaneously by only using linear passive components because of the MDOF adjustable stiffness property. Therefore, with low costing and energy consumption, the proposed novel isolation platform can provide the improvement of vibration suppression in various engineering practices.

The formulation used by the most of studies on an elastic torus are either Reissner mixed formulation or Novozhilov's complex-form one, however, for vibration and some displacement boundary related problem of the torus, application of those formulations has encountered great difficulty. It is highly demanded to have a displacement-type formulation for the torus. In this paper, I will simulate some typical problems and free vibration of the torus. The numerical results are verified by both finite element analysis and H. Reissner's formulation. My investigations show that both deformation and stress response of an elastic torus are sensitive to the radius ratio, and suggest that the analysis of a torus should be done by using the bending theory of a shell, and also reveal that the inner torus is stronger than outer torus due to the property of their Gaussian curvature. Regarding the free vibration of the torus, our analysis indicates that both initial in u and w direction must be included otherwise will cause big errors in eigenfrequency.

Intelligent fault diagnosis techniques have replaced the time-consuming and unreliable human analysis, increasing the efficiency of fault diagnosis. Deep learning model can improve the accuracy of intelligent fault diagnosis with the help of its multilayer nonlinear mapping ability. This paper has proposed a novel method named Deep Convolutional Neural Networks with Wide First-layer Kernels (WDCNN). The proposed method uses raw vibration signals as input (data augmentation is used to generate more inputs), and uses the wide kernels in first convolutional layer for extracting feature and suppressing high frequency noise. Small convolutional kernels in the preceding layers are used for multilayer nonlinear mapping. AdaBN is implemented to improve the domain adaptation ability of the model. The proposed model addresses the problem that currently, the accuracy of CNN applied to fault diagnosis is not very high. WDCNN can not only achieve 100% classification accuracy on normal signals, but also outperform state of the art DNN model which is based on frequency features under different working load and noisy environment.

This paper presents experimental identification and vibration suppression of a flexible manipulator with non-collocated piezoelectric actuators and strain sensors using optimal multi-poles placement control. To precisely identify the system model, a reduced order transfer function with relocated zeros is proposed, and a first-order inertia element is added to the model to compensate the non-collocation. Comparisons show the identified model match closely with the experimental results both in the time and frequency domains, and a fit of 97.2% is achieved. Based on the identified model, a full-state multi-poles placement controller is designed, and the optimal locations of the closed loop poles are determined. The feasibility of the proposed controller is validated by simulations. Moreover, the controller is tested for different locations of the closed loop poles, and an excellent performance of the optimal locations of the closed loop poles is shown. Finally, the effectiveness of the proposed controller is demonstrated by experiments. Results show that the vibrations of the expected modes are significantly diminished. Besides, vibrations of the higher modes are also slightly suppressed. Accordingly, multi-mode vibrations of the manipulator are well attenuated, and the tip displacement converges quickly with the proposed method.

Model-based fault diagnosis serves as a powerful technique for addressing fault detection and isolation issues in control systems. However, diagnosing faults in closed-loop control systems is more challenging due to their inherent robustness. This paper aims to detect and isolate actuator and sensor faults in the cascade electro-hydraulic control system of a turbofan engine. Based on the fault characteristics, we design a robust unknown perturbation decoupling residual generator and an optimal fault observer specifically for the inner and outer control loops to detect potential faults. To locate the faults, we analyze the steady-state propagation laws of actuator and sensor faults within the loops using the final value theorem. Based on this, we establish the minimal-dimensional fault influence distribution matrix specific to the cascade turbofan engine control system. Subsequently, we construct the normalized residual vectors and monitor its vector angles against each row of the fault influence distribution matrix to isolate faults. Experiments conducted on an electro-hydraulic test bench demonstrate that our proposed method can accurately locate four typical faults of actuators and sensors within the cascade electro-hydraulic control system. This study enriches the existing fault isolation methods for complex dynamic systems, and lays the foundation for guiding component repair and maintenance.

Optical tweezers offer a non-contact method for selecting single cells and translocating them from one microenvironment to another. We have characterized the optical tweezing of yeast S. cerevisiae and can manipulate single cells at velocities up to 0.77 mm/s using laser powers of 40 mW from a 785 nm diode laser. We have fabricated and tested three cell isolation devices; a micropipette, a PDMS chip and laser machined fused silica chip and we have isolated single bacteria, yeast and cyanobacteria cells. The most effective isolation was achieved in PDMS chip, where single yeast cells were grown and observed for 18 hours without contamination. The duration of budding in S. cerevisiae was not affected by the laser parameters used, but the time from tweezing until the first budding event began increased with increase laser energy (laser power x time). Cells tweezed using 25 mW for 1 minute were viable after isolation. We have constructed a micro-consortium of yeast cells, and a co-culture of yeast and bacteria, using optical tweezers in combination with the PDMS network of channels and isolation chambers, which may impact on both industrial biotechnology and understanding pathogen dynamics.

This paper delves into the challenge of controlling the multiple synchronizations in mechatronic vibration machines featuring a duo of unbalanced rotors. Its primary aim is to not only attain specific rotation speeds but also to ensure the desired phase shift between these rotors. To tackle this issue, the paper employs a control law integrating cross-couplings. It conducts an analysis of the dynamics of a simplified linearized system, showcasing the robustness of the control system across varying plant parameters. Additionally, the paper outlines experimental findings from a multi-resonance vibrational laboratory setup. These findings effectively demonstrate the effectiveness of the proposed approach and highlight its potential applications. Crucially, the paper provides experimental evidence illustrating the capability to control vibration fields on the operational platform, a vital aspect pertinent to vibration transportation and bulk material mixing processes.

Imperfection sensitivity of large amplitude vibration of curved single-walled carbon nanotubes (SWCNTs) is considered in this study. The SWCNT is modeled as a Timoshenko nano-beam and its curved shape is included as an initial geometric imperfection term in the displacement field. Geometric nonlinearities of von Kármán type and nonlocal elasticity theory of Eringen are employed to derive governing equations of motion of nano-beam. Spatial discretization of governing equations and associated boundary conditions is performed using differential quadrature (DQ) method and the corresponding nonlinear eigenvalue problem is iteratively solved. Effects of value and location of the geometric imperfection, and the nonlocal small-scale parameter on the nonlinear frequency ratio and imperfection sensitivity of a curved SWCNT for various boundary conditions are investigated. The results show that the geometric imperfection plays a significant role in the nonlinear vibration characteristics of curved SWCNTs.

Extracellular vesicles (EVs) carry diverse cargo of DNA, RNA, proteins, and lipids. They are crucial mediators for regulating various functions through interactions with the host. Recent investigations suggest that EVs or their constituent proteins derived from probiotics could improve the effectiveness of probiotic strains in disease management compared to probiotic strains themselves. This enhanced effec-tiveness arises from their innate antimicrobial properties, which can specifically target pathogenic mol-ecules or cells, in addition to their significant ability to regulate immune responses. Significantly, previous studies have highlighted the inhibitory potential of Lactobacillus crispatus PMC201 against Mycobacterium tuberculosis. In this study, we focused on optimizing isolation conditions for studying extracellular vesicles and proteins from anti-TB potent strains. We provide analysis results based on food-grade media (FGM) optimization and isolation methods. Nanoparticle tracking analysis (NTA) was used to determine se-cretion patterns. Proteomic analysis was performed by liquid chromatography and tandem mass spec-trometry (LC-MS/MS). A total of 64 proteins were identified in the L. crispatus PMC201 strain, particularly enriched in s-layer and splX. Questions remain about their relevance to anti-TB. This anti-tuber strain has potential as a therapeutic agent, given its significant role in intercellular killing. Further studies are needed to investigate its mechanisms of action.

Bats are of significant interest as reservoirs for various zoonotic viruses with high diversity. During the past two decades, many herpesviruses have been identified in various bats worldwide by genetic approaches, whereas there have been few reports on the isolation of infectious herpesviruses. Herein, we report the prevalence of herpesvirus infection of bats captured in Zambia and genetic characterization of novel gammaherpesviruses isolated from Macronycteris vittatus bats. By our PCR screening, herpesvirus genomes were detected in 29.2% (7/24) of Rousettus aegyptiacus, 78.1% (82/105) of Macronycteris vittatus, and 100% (1/1) of Hipposideros caffer bats in Zambia. Phylogenetic analyses of the detected herpesvirus genomes revealed that the Zambian bat herpesviruses were divided into seven betaherpesvirus groups and five gammaherpesvirus groups. Two infectious strains of a novel gammaherpesvirus, tentatively named Macronycteris gammaherpesvirus 1 (MaGHV1), were successfully isolated from Macronycteris vittatus bats, and their complete genomes were sequenced. The genome of MaGHV1 encoded 79 open reading frames, and phylogenic analyses of the DNA polymerase and glycoprotein B demonstrated that MaGHV1 formed an independent lineage sharing a common origin with other bat-derived gammaherpesviruses. Our findings provide new information regarding the genetic diversity of herpesviruses maintained in African bats.

The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A (2.10 ± 0.70 g/L) and B (1.54 ± 0.17 g/L) produced more biomass than the ones isolated from Location C (0.43 ± 0.02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine (2.55 ± 0.74 g/L) as compared to of commercial origin (1.06 ± 0.57 g/L). Glycerol (2.43 ± 0.56 g/L) was a better carbon source than glucose (1.84 ± 0.57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated (4.21 ± 2.96%) and omega-6 (0.68 ± 0.59%) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35.65 ± 1.73%) and total omega-3 FAs (40.39 ± 2.39%), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated (18.38 ± 6.27%) long chain (57.43 ± 8.27%) FAs.

Taking a certain type of test centrifuge as the research object, the finite element model of the test centrifuge is established, the vibration characteristics and aerodynamic performance of the test centrifuge are analyzed, and the structural optimization design of the test centrifuge is carried out. In this paper, the load is applied according to the actual working condition of a certain type of test centrifuge. The vibration of the mounting seat of the test centrifuge is analyzed and the structure of the mounting seat is optimized. After optimization, the vibration of the mounting seat is 77.38 % lower than that of the original mounting seat. Then, the aerodynamic analysis of the test centrifuge is carried out. The analysis results show that the test centrifuge moves more smoothly under the whole package shell and the steady flow shell, and the resistance decreases and the shaft load decreases. Finally, the steady flow shell of the test centrifuge is optimized. The analysis shows that the increase of the width of the steady flow shell can reduce the resistance coefficient, which is helpful to the stability of the test centrifuge during operation and reduces the unbalanced response of the system caused by air resistance.

In future automated vehicles we will often engage in non-driving tasks and will not watch the road. This will affect postural stabilization and may elicit discomfort or even motion sickness in dynamic driving. Future vehicles shall accommodate this by properly designed seats and interiors whereas comfortable vehicle motion shall be achieved with smooth driving styles and well de-signed (active) suspensions. To support research and development in dynamic comfort, this paper presents validation of a multi-segment full body human model including visuo-vestibular and muscle spindle feedback for postural stabilization. Dynamic driving is evaluated using a “sicken-ing drive” including a 0.2 Hz 4 m/s2 slalom. Vibration transmission is evaluated with compliant automotive seats, applying 3D platform motion and evaluating 3D translation and rotation of pelvis, trunk and head. The model matches human motion in dynamic driving and reproduces fore-aft, lateral and vertical oscillations. Visuo-vestibular and muscle spindle feedback are shown to be essential in particular for head-neck stabilization. Active leg muscle control at the hips and knees is shown to be essential to stabilize the trunk in the high amplitude slalom condition but not in low amplitude horizontal vibrations. However, active leg muscle control can strongly affect 4-6 Hz vertical vibration transmission. Compared to the vibration tests, the dynamic driving tests show enlarged postural control gains to minimize trunk and head roll and pitch, and to align head yaw with the driving direction. Human modelling can create the required insights to achieve breakthrough comfort enhance-ments while enabling efficient development for a wide range of driving conditions, body sizes and other factors. Hence, modelling human postural control can accelerate innovation of seats and vehicle motion control strategies for (automated) vehicles.

The formulation used by most of the studies on an elastic torus are either Reissner mixed formulation or Novozhilov's complex-form one, however, for vibration and some displacement boundary related problem of a torus, those formulations face a great challenge. It is highly demanded to have a displacement-type formulation for the torus. In this paper, I will carry on my previous work [ B.H. Sun, Closed-form solution of axisymmetric slender elastic toroidal shells. J. of Engineering Mechanics, 136 (2010) 1281-1288.], and with the help of my own maple code, I am able to simulate some typical problems and free vibration of the torus. The numerical results are verified by both finite element analysis and H. Reissner's formulation. My investigations show that both deformation and stress response of an elastic torus are sensitive to the radius ratio, and suggest that the analysis of a torus should be done by using the bending theory of a shell, and also reveal that the inner torus is stronger than outer torus due to the property of their Gaussian curvature. Regarding the free vibration of a torus, our analysis indicates that both initial in u and w direction must be included otherwise will cause big errors in eigenfrequency. One of the most intestine discovery is that the crowns of a torus are the turning point of the Gaussian curvature at the crown where the mechanics' response of inner and outer torus is almost separated.

A new-type vibration sensor based on a fiber Bragg grating combining with a special structure-packaged design is proposed for monitoring the mechanical vibration signals. Three different sensing structures including the film squeeze type, new film squeeze type and elastic tape squeeze type are proposed for measuring the vibration signals with the frequency range from tens to thousands of Hz. In the comparison of experimental results, the new film squeeze structure has a nice sensing performance in the range from 100 to 1000 Hz with the sensitivity of 35 mV/V. For the elastic tape squeeze structure, the elastic tape is designed to encapsulate the optical fiber with a good frequency response from 1100 to 3100 Hz. In addition, by using the new film squeeze structure to measure the steady-state and non-steady-state vibration signals, the spectral components of sensing signals are analyzed by using the wavelet transformation for confirming the testing signals. These vibration fiber sensors can be applied in the measurement of high-end manufacture-facility vibration or earthquake vibrations etc.

The distributed long-range sensing system using the standard telecommunication single-mode optical fiber in a function of a distributed sensor for sensing of mechanical vibrations is described. Various events generating vibrations such as walking or running person, moving cars, trains and others can be detected, localized and classified. The sensor and related sensing system components were designed and constructed and the system was tested both in the laboratory and in the real situation with 88 km telecom optical link, and the results are presented.

Force reduction is one of the most important benefit of applying ultrasonic vibration on milling. However, most of studies so far are limited to experimental investigation. In the current study, an analytical predictive model on cutting forces in ultrasonic vibration-assisted milling is proposed. The three types of tool-workpiece criteria are considered based on the instantaneous position and velocity of tool center. Type I criterion indicates that there is no contact if the instantaneous velocity is opposite to tool rotation direction. Type II criterion checks whether the vibration displacement is larger than the instantaneous uncut chip thickness. Type III criterion considers the overlaps between current and previous tool paths due to vibration. If none of these criteria is satisfied, milling forces are nonzero. Then the calculation is performed by transforming milling and tool geometry configuration to orthogonal cutting at each instant. The orthogonal cutting forces are predicted through the exhaustive search of shear angle and calculation of shear flow stress on tool-chip interface. The axial force is then calculated based on tool geometry, and the milling forces in feed, cutting, and axial directions are calculated after coordinate transformation. The proposed predictive force model in ultrasonic vibration-assisted milling is validated through comparison to experimental measurements on Aluminum alloy 2A12. The predicted values are able to match the measured milling forces with high accuracy of average difference of 13.6% in feed direction and 13.8% in cutting direction.

Since fine powders tend strongly to adhesion and agglomeration, their processing with conventional methods is difficult or impossible. Typically, in order to enable the handling of fine powders, chemicals are added to increase the flowability and reduce adhesion. This contribution shows that instead of additives also vibrations can be used to increase the flowability, to reduce adhesion and cohesion, and thus to enable or improve processes such as precision dosing, mixing, and transport of very fine powders. The methods for manipulating powder properties are described in detail and prototypes for experimental studies are presented. It is shown that the handling of fine powders can be improved by using low-frequency, high-frequency or a combination of low- and high-frequency vibration.

Natural phytochemicals in foods, including anthocyanins, can play an important role in human health. Anthocyanins have been reported to cause many various useful effects, such as reducing cancer cell proliferation, regulating blood pressure, preventing tumour formation, improving eyesight, and preventing diabetes. In this study, we aimed to reveal the qualitative anthocyanin content, antiproliferative and antimicrobial effects of different extracts derived from Vaccinium myrtillus, V. corymbosum, Sambucus nigra and Aronia melanocarpa. The anthocyanin content of the plants mentioned in the study was characterized after the freeze-drying process. MTT assay was used to determine the antiproliferative effect of extracts on cancer cells. Antimicrobial effects of extracts were studied on typical and clinical strains of 5 different bacteria. As a result, the anthocyanin content in the extracts obtained was determined to be quite good with the freeze-drying method, and it was also determined that the extracts had various levels of antiproliferative and antibacterial effects.

(1) Background: Group A rotaviruses (RVAs) are the primary cause of severe intestinal diseases in piglets. Porcine rotaviruses (PoRVs) are widely prevalent in Chinese farms, resulting in significant economic losses to the livestock industry. However, isolation of PoRVs is challenging, and their pathogenicity in piglets is not well understood. (2) Methods: We conduct clinical testing on a farm in Jiangsu Province, China, and isolate strains of porcine rotavirus by continuously passaging on MA104 cells. Subsequently, the pathogenicity of the isolated strain in piglets was investigated.The piglets of the PoRV-infection group were orally inoculated with 1 mL of 1.0 × 10⁶ TCID50 PoRV, whereas those of the mock-infection group were fed with an equivalent amount of DMEM. (3) Results: Fecal samples were collected from 28 piglets, all of which tested positive for RVAs. A G5P[23] genotype porcine rotavirus strain was successfully isolated from one of the positive samples and named RVA/Pig/China/JS/2023/G5P[23](JS). The genomic constellation of this strain was G5-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Sequence analysis revealed that the genes VP3, VP7, NSP2, and NSP4 of the JS strain were closely related to human RVAs, whereas the remaining gene segments were closely related to porcine RVAs, indicating a reassortment between porcine and human strains. Furthermore, infection of 15-day-old piglets with the JS strain resulted in a diarrheal rate of 100% (8 of 8) and mortality rate of 37.5% (3 of 8).(4) Conclusion: The isolated G5P [23] genotype rotavirus strain, which exhibited strong pathogenicity in piglets, may have resulted from recombination between porcine and human strains. It may serve as a potential candidate strain for developing vaccines, and its immunogenicity can be tested in future studies.

Objectives: One of the home-made herbal remedy being traditionally used against gout is evaluated to identify any new and characteristic marker compound which may be used as characteristic analytical marker to standardize this polyherbal remedy. Materials and method: A distinct and remarkable peak in the HPLC chromatogram of remedy was identified which was further isolated by fraction collector. This fraction was then evaporated and characterized for structure elucidation using set of various techniques such as RP-HPLC,UV, FTIR, TLC, EIMS and NMR. Results: The compound characterized was non polar compound, ester of betulin and palmitoleic acid. Although the remedy has been standardized using set of four markers as discussed in literature but this newly developed marker compound can be used to further standardize this remedy and other herbal preparations comprising of this marker compound.Conclusion: Hence the isolated compound can be used as novel analytical marker in the standardization of this herbal remedy and the other herbal preparations containing such marker compound

Terminalia arjuna possesses significant cardio protective, antidiabetic and antioxidant properties as these properties are described in Ayurveda. In the present study, three flavonoids were isolated through the separation and chromatographic purification of the whole plant material of T. arjuna. Spectroscopic characterization identified one of them as a new flavonoid “Terminalone A (1)” and two known flavonoids i.e. 6-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one (2) and 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one (3). The bioactivity studies showed considerable antibacterial and antioxidant (DPPH was used as a scavenger) potential for all the three compounds 1-3 where the compound 1 showed strong antibacterial and antioxidant activity.

Ochna kibbiensis (Family: Ochnaceae) has been employed in ethnomedicine for the treatment of malaria, and inflammation among others. The aim of this study was to isolate and characterize prophylactic antimalarial agents from the leaves of O. kibbiensis against Plasmodium berghei, in vivo and in silico. The median lethal dose (LD50) of the methanol extract and its fractions (n-hexane, dichloromethane, ethylacetate and n-butanol) was determined according to Lorke’s method while the antimalarial effect of the extract and its fractions was investigated according to the method described by Peters prophylactic test using Chloroquine-sensitive Plasmodium berghei (NK65). All the extract/fractions exhibited LD50 values ≥ 5000 mg/kg with the exception of the n-butanol fraction (1702.94 mg/kg) which indicate that the plant is non-toxic. Dichloromethane fraction exhibited significant (p<0.05) and dose-dependent prophylactic effect with 47.62, 85.12 and 100.0 % prophylaxis (at 500, 250 & 125 mg/kg) while the least effect was observed by the n-butanol fraction with percentage prophylaxis of 64.29 and 76.19, respectively; the standard drug, pyrimethamine had 95.24 % prophylaxis. Based on the result obtained, dichloromethane fraction of O. kibbiensis was subjected to chromatographic purification which led to the isolation of a mixture of two compounds identified as stigmasterol and β-sitosterol by analysis of the NMR spectral data and comparison with existing literature; the compounds exhibited good binding affinities (- 5.129 and - 4.889 kcal/mol) against pfLDH and favorable ADMET profile. In conclusion, the leaf of O. kibbiensis have demonstrated a significant prophylactic antimalarial activity and the two known steroids (stigmasterol and β-sitosterol) isolated from the dichloromethane fraction for the first time.

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically and mycologically significant to find such rare zygomycetous fungi in such specific habitats.

This paper describes a novel thruster fault-tolerant control system (FTC) for open-frame remotely operated vehicles (ROVs). The proposed FTC consists of two subsystems: a model-free thruster fault detection and isolation subsystem (FDI) and a fault accommodation subsystem (FA). The FDI subsystem employs fault detection units (FDUs), associated with each thruster, to monitor their state. The robust, reliable and adaptive FDUs use a model-free pattern recognition neural network (PRNN) to detect internal and external faulty states of the thrusters in real time. The FA subsystem combines information provided by the FDI subsystem with predefined, user-configurable actions to accommodate partial and total faults and to perform an appropriate control reallocation. Software-level actions include penalisation of faulty thrusters in solution of control allocation problem and reallocation of control energy among the operable thrusters. Hardware-level actions include power isolation of faulty thrusters (total faults only) such that the entire ROV power system is not compromised. The proposed FTC system is implemented as a LabVIEW virtual instrument (VI) and evaluated in virtual (simulated) and real-world environments. The proposed FTC module can be used for open frame ROVs with up to 12 thrusters: eight horizontal thrusters configured in two horizontal layers of four thrusters each, and four vertical thrusters configured in one vertical layer. Results from both environments show that the ROV control system, enhanced with the FDI and FA subsystems, is capable of maintaining full 6 DOF control of ROV in the presence of up to 6 simultaneous total faults in the thrusters. With the FDI and FA subsystems in place the control energy distribution of the healthy thrusters is optimised so that the ROV can still operate in difficult conditions under fault scenarios.

Rice is currently the most important food crop in the world and we are only just beginning to study the bacterial associated microbiome. It is of importance to perform screenings of the core rice microbiota and also to develop new plant-microbe models and simplified communities for increasing our understanding about the formation and function of its microbiome. In order to begin to address this aspect, we have performed the isolation of hundreds bacterial isolates obtained from endorhizosphere of two rice cultivars from Venezuela. The validation of plant-growth promoting bacterial activities in vitro has led us to select and characterize 15 isolates for in planta studies such as germination test, endophytism ability and plant growth promotion. Consequently, a set of 10 isolates was selected for the set-up of an endophytic consortium as a simplified model of the natural rice bacterial endomicrobiota. Upon inoculation, the colonization and abundance of each strain within the rice roots was tracked by a culture-independent technique in gnotobiotic conditions in a 30 days period. Four strains belonging to Pseudomonas, Agrobacterium and Delftia genera have shown a promising capacity for colonizing and coexistence in root tissues. On the other hand, a bacterial community taxonomic profiling of the rhizosphere and the endorhizosphere of both cultivars were obtained and are discussed. This study is part of a growing body of research on core crops microbiome and simplified microbiomes, which strengthens the formation process of the endophytic community leading to a better understanding of the rice microbiome.

This paper studied the dynamic characteristics of the deployment process of the replaceable interface mast and the vibration generated at the end of the mast. The interface is discretized by the absolute nodal coordinate thin shell element with gradient reduction, and the three-dimensional motion of the reconfigurable rigid body module is described by the natural coordinate method. The dynamic equations of the interface and the reconfigurable module without external constraints are established by the principle of virtual work. Finally, the dynamic models of each part are assembled into the dynamic model of the mast system deployment process through the system constraint equation. According to the established dynamic model of the mast system deployment process, the dynamic behavior changes of the replaceable interface mast are analyzed. At the same time, the differences in the deployment behavior of the replaceable interface mast under different system configuration schemes, flexible interface geometric parameters, and different driving laws are studied and compared. It provides guidance for the scheme configuration of the mast system, the structural design of the interface, and the deployment motion planning of the mast. According to the physical prototype and the assembly of the mast system model, the deployment process of the replaceable interface mast is experimentally analyzed. It shows that the established dynamic model of the mast system can correctly analyze the dynamic characteristics of the deployment behavior of the replaceable interface mast, which provides a reference for the design and behavior analysis of the mast system.

Micro-gyroscopes based on the Coriolis principle are widely employed in inertial navigation, motion control, and vibration analysis applications. This paper presents our main contributions which include a novel dual-frame optomechanical gyroscope, a unique photonic crystal cavity design, and advanced numerical simulation and optimization methods. The proposed design utilizes an optical cavity formed between dual oscillating frames, whereby input rotation induces a measurable phase shift via optomechanical coupling. Actuation of the frames is achieved electrostatically via an interdigitated comb-drive design. Through theoretical modeling based on cavity optomechanics and finite element simulation, the operating principle and performance parameters are evaluated in detail. Results indicate an expected angular rate sensitivity of 22.8 mV/°/s and angle random walk of 7.1×10-5 °/h1/2, representing superior precision than to existing micro-electromechanical systems gyroscopes of comparable scale. Detailed analysis of the optomechanical transduction mechanism suggests this dual-frame approach could enable angular vibration detection with resolution exceeding state-of-the-art solutions.

Distributed fiber optic vibration sensors based on Φ-OTDR using Rayleigh backscattering of highly coherent light source pulses are very popular type in the area of sensors due to many attractive features, mainly due to its distributed manner of sensing, i.e. they can operate as thousands of local sensors simultaneously. The paper summarizes and describes several important modifications of the original sensor scheme to enhance its key performance parameters and to extend its application potential. A multi-fiber sensor form was proposed. Single- and two-fiber sensors were designed, constructed and tested both in the laboratory and in a real environment. Variants of two-fiber sensor utilizing optical switches for the sensor flexibility enhancement are proposed and their applications are described.

At the end of mitosis the Mitotic Exit Network (MEN) pathway triggers complex tasks which mainly include the spindle disassembly and the nuclear envelopes assembly. In the course of telophase, which often lasts less than an hour and corresponds to only about 2% of the entire cell cycle’s duration, spatial and temporal cues are integrated to ensure that cytokinesis occurs after the genome has partitioned between mother and daughter cells. From the end of anaphase through telophase, sequential components of a Ras-like GTPase signaling pathway are controlled by a set of different spatial and temporal signals. Successful propagation of these signals through multi-step transduction requires a remarkable sequential coordination. By considering that cells lacking proper MEN function fail to exit from mitosis, I argue that in a hybrid genome impaired coordination between two diverged MENs is prone to result in critical mitotic defects, from late anaphase through telophase. The so-called HyMEN model of hybrid incompatibility depicted here can be regarded as an extension of the Bateson-Dobzhansky-Muller model of speciation, centered on the MEN.

For engines operating with heavy fuel oil (HFO), the nozzle rings of the turbocharger turbines are prone to severe degradation in terms of contamination with unburned fuel deposits. This contamination may lead to increased excitation of blade resonances. A previous study provides technical guidelines on how to extract the relevant information from the pulsation spectra by means of a single probe installed away from the turbine trailing edge and some sound experimental proofs of integral mode turbine vibration detection. These theoretical discussions only allude to the effects of mistuning and interferences due to classical blade passing frequencies on sound radiation patterns emitted by integral blade vibration modes. In this paper, both effects are thoroughly discussed. Combining the knowledge of theoretical study and further experimental results, the application range of this blade vibration detection method can be remarkably extended.

In thermal power plants equipped with air-cooled condensers, axial cooling fans operate under the influence of ambient flow fields. Under inlet cross-flow conditions, the resultant asymmetric flow field is known to introduce additional harmonic forces to the fan blades. This effect has previously been studied only numerically or using blade mounted strain gauges. For this study, Laser Scanning Vibrometry was used to assess fan blade vibration under inlet cross-flow conditions in an adapted fan test rig inside a wind tunnel test section. Two co-rotating laser beams scanned a low pressure axial fan, resulting in spectral, phase resolved surface vibration patterns of the fan blades. Two distinct operating points were examined, with and without inlet cross-flow influence. While almost identical fan vibration patterns were found for both reference operating points, overall blade vibration increased by 100% at low fan flow rate due to cross-flow, and by 20% at high fan flow rate. While numerically predicted natural frequency modes could be confirmed from experimental data as minor peaks in the vibration amplitude spectrum, they were not excited significantly by cross-flow. Instead, primarily higher rotation rate harmonics were amplified, i.a. a synchronous blade tip flapping was strongly excited at the blade pass frequency.

Abstract: There are a large number of cross-linked chemical bonds such as tight disulfide bonds, hydrogen bonds,and salt bonds in keratin, which makes it difficult to break the structural stability of the protein using conventional methods. The degradation of feather waste using keratinases produced by microorganisms has the advantage of being efficient and environmentally friendly. In this study, 23 strains capable of producing transparent circles were obtained via preliminary screening with skim milk as the sole carbon and nitrogen source, and 9 strains capable of degrading feathers were obtained via rescreening with feathers as the sole carbon and nitrogen source. Finally, through the determination of enzyme activity, the strain CY-A with a high ability to degrade feathers was obtained. The strain was identified as Bacillus tequilensis via morphological observation, physiological and biochemical identification, and 16 S rRNA molecular biology. The keratinase gene (bta) of Bacillus tequila was cloned, and its full-length gene sequence was 1110 bp, containing an open reading frame of 1089, encoding 369 amino acids (GeneBank accession number OR733336). The physicochemical properties, secondary structure, and tertiary structure of the recombinant protein were analyzed using bioinformatics tools such as ProtParm, SOPMA, MEGA, and SWISS-MODELS. It was found that the relative molecular mass was 37.953 kD and that the isoelectric point was 8.48. The protein was a hydrophilic protein. The keratinase bta gene was ligated to the expression vector pET28a(+) using T4 ligase, and the recombination expression plasmid pET28-bta was constructed and transformed into E. coli BL21(DE3) to obtain recombinant engineered bacteria. The expressed keratinase was purified using a Ni2+ affinity chromatography column to obtain recombinant bta keratinase bta with a molecular weight of 37.953 kD. The identification and characterization of this keratinase gene provides some theoretical basis for the further enhancement of keratinase activity by means of genetic engineering.

The extraction of cell cultures from fresh tissue is a process that requires significant computa-tional resources and is associated with substantial overheads. The objective of our research is to de-vise a rapid, straightforward, and efficient technique for the cultivation of neuronal cells derived from frozen biological material. We utilized freshly isolated hippocampus and cerebral cortex tissue from rats, in addition to whole brain tissue. This tissue underwent digestion and processing to yield a cellular suspension. This suspension was subsequently cryopreserved using a variety of cryopro-tectants: CTS™ Synth-a-Freeze™, SpermFreeze®, and a medium supplemented with 10% DMSO. CTS™ Synth-a-Freeze™ is a commercially available cryoprotectant typically employed in the preservation of cellular cultures, while the addition of 10% DMSO to the medium is a conventional technique for the cryopreservation of cells to mitigate damage. The innovative aspect of our meth-odology is the application of SpermFreeze®, a product used for the cryopreservation of sperm in in vitro clinics. We postulated that SpermFreeze® might enhance the viability and quality of the cryo-preserved neuronal cells, given the delicate and energetically demanding nature of both sperm and neuronal cells. The ultimate objective was to ascertain whether a cryoprotectant formulated for sperm could augment the post-thawing efficacy of our nervous system cells. This investigation intro-duces an innovative methodology for the optimization of neuronal cell isolation and cryopreserva-tion from animal-derived tissues.

MicroRNAs (miRNAs) within the exosomes are short non-coding RNAs that are associated with many diseases including cancers and are crucial players in regulating gene expression and regula-tion. Owing to their potential role as emerging biomarkers, efficient isolation, and quantification methods of miRNA from the exosomes in the cell’s supernatant are complicated and challenging. The quantitative PCR method is the gold standard for miRNA identification and estimation. Nucleic acid-based assays are expensive, labour-intensive, and require expertise. In this work, we demon-strated an aptamer-based magnetic separation of the exosomes, and quantification of the miRNA using a fluorescence switching assay. The fluorescence assay relies on the fluorescence of the fluorophore labelled cDNA quenched by carbon nanomaterials coated on magnetic beads (OFF state). When the target miRNA210 is introduced to the system, the cDNA detaches from the quenching surface. As a result, the fluorescence intensity increases (ON state) with increasing miRNA concentration within the dynamic range of 0-100 nM. This method can detect miR210 as low as 5 pM. This method does not exhibit any cross-reactivity with other closely related miRNAs. The method was validated by spiking miRNA with the standard RT-PCR method using SYBR green as a fluorescent probe. This method can be used for the minimally invasive detection of cancer biomarkers in breast cancer patients.

Faba bean (Vicia faba L.) is a partially outcrossing species requiring an isolation distance to maintain genetic purity when more than one variety is grown in field conditions. This information is crucial for seed growers and faba bean breeders. A study was conducted at the University of Sydney’s Plant Breeding Institute, Narrabri over two years to examine the extent of natural outcrossing using a creamy white flower characteristic as a morphological marker, which is controlled by a single recessive gene. The white flowered genotype (IX225c) was grown in paired rows of 150 m length in four directions from a central 480 m2 plot of the normal flowered genotype PBA Warda. A beehive was placed in the central plot at flowering time and natural pollination was allowed. At maturity, seed samples were taken from the white flowered genotype at designated intervals along each axis and 100 seeds from each sample were grown in the glasshouse/birdcage to the 4-5 leaf stage and the proportion of plants displaying stipule spot pigmentation (normal flower colour and spotted stipule are linked) was used to determine the percentage of outcrossing. Maximum outcrossing of 2.28% occurred where both genotypes were grown side by side (0 m) and the degree of outcrossing decreased as the distance along each axis from the central plot increased. At 6 m distance the outcrossing was less than 1%, however on occasion it increased to 1% beyond a distance of 100 m, indicating the volatile and unpredictable nature of bee flights. Distance had the major effect on outcrossing, but direction and its interaction had no effect. The results suggest that to limit outcrossing to below 0.5%, a distance of more than 150 m between plots of different faba beans cultivars would be required. It also indicated that Australian faba bean genotypes are mostly self-fertile and a relatively narrow isolation distance will ensure self-fertilization in seed production and breeding programs.

Proso millet starch (PMS) as an unconventional and underutilized cereal starch is becoming increasingly popular worldwide due to its health-promoting properties. This review summarizes research progress in the isolation, characterization, modification, and applications of PMS. PMS can be isolated from proso millet grains by acidic, alkaline, or enzymatic extraction. PMS exhibits typical A-type polymorphic diffraction patterns and shows polygonal and spherical granular structures with a granule size of 0.3-17 µm. PMS is modified by chemical, physical, and biological methods. The native and modified PMS are analyzed for swelling power, solubility, pasting properties, thermal properties, retrogradation, freeze-thaw stability, and in vitro digestibility. The improved physicochemical, structural, and functional properties and digestibility of modified PMS are discussed in terms of their suitability for specific applications. The potential applications of native and modified PMS in food and non-food products are presented. Future prospects for research and commercial use of PMS in the food industry are also highlighted.

Abstract: Theoretical and non-dimensional investigations have been performed to study the vibration control potential of approaches that can be based on viscoelastic but also on endochronic elements. The latter are known from the endochronic theory of plasticity and provide the possibility to establish rate-independent schemes for vibration control. The main question that has to be answered is: Can rate-independent damping be efficiently used to reduce mechanical vibrations? To answer this question non-dimensional models for dynamical systems are derived and analyzed numerically in time-domain as well as in frequency-domain. The results are used to compare the performance of an optimally tuned endochronic absorber to the performance of an optimally tuned dynamic absorber with viscoelastic damping. Based on a novel closed form representation for non-linear systems with endochronic elements, it has been possible to prove that rate-independent control of vibration results in an overall control profit that is close to the control profit obtained by the application of well-established approaches. It has also been found that the new concept is advantageous if anti-resonances have to be considered in broadband vibration control. Based on these novel findings, a practical realization in the context of active vibration control is proposed in which the rate-independent control law is implemented on an appropriate signal processing hardware.

This paper provides a proof of concept for a linear electric generator that can be used to harvest energy from various sources of linear motion, such as vibrations, free-piston engines and wave energy. The generator can be used to power small electronic devices, such as sensors, or charge household batteries. Literature was reviewed to develop an understanding about the applications, control methods, excitation methods and mechanics of rotating and linear electric machines. A bidirectional, two-sided linear machine was designed with two stator cores and a single mover core. The stator windings and mover winding can be independently excited, allowing for three modes: no mover excitation, DC excited mover, and AC excited mover. Simulations results showed that the magnetic flux generated by DC excited stator cores were concentrated in the centre of the mover core. The use of two stator cores eliminates lateral flux in the mover core when it is not excited, minimising attraction and repulsion forces. Parametric analysis showed that flux cutting occurred in all operation modes, verifying that the generator will produce power when operating. Hardware tests produced an output current when the machine is electrically and mechanically excited, verifying the proposed design.

In order to reduce the vibration of the transporter and improve the stability of the transporter, the vibration characteristics and key structure optimization research of the mountain orchard transporter are carried out. In this paper, the vibration of electric monorail transporters under different working conditions is tested, and the causes of vibration generated by monorail transporter during operation are explored. The three-dimensional model of the trailer and transmission box is established using Solidworks, and the modal vibration modes of the structure are analyzed theoretically. The modal test system is used for modal test analysis of trailers and transmission boxes. By comparing the results of finite element and experimental modal analysis, the second-order frequency of the trailer is close to the motor's rotating excitation frequency of 50 Hz at rated speed, which is easy to generate resonance. By optimizing the structure of the trailer, the second and third natural frequencies are increased to 54.79 Hz and 58.35 Hz respectively, which avoid the resonance of the trailer during operation and effectively reduce the vibration generated during the transportation of the trailer. Through vibration testing of the optimized electric monorail transporter, the results of vibration testing before and after structural optimization are compared and analyzed, and it is found that the vibration amplitudes of the transporter in X, Y and Z directions are reduced by 0.308m/s², 0.351m/s² and 0.334m/s² respectively, the running stability of the conveyer is improved.

Determining the vibration environment is crucial to analyzing a design of any mechanical system, especially such dynamic systems as sounding rockets. Accuracy of measurement using accelerometers could be improved by application of mechanical vibration filtering and amplifying devices. This work presents a theoretical description of a tunable filter and amplifier. Principle of work is provided as well as results from application of the device on a sounding rocket are provided. It is shown that implementation of such devices allowed for enhancing the accuracy of acceleration measurements. Conclusions on future implementations are also provided.

Malassezia species are fastidious and slow-growing yeasts whose isolation from polymicrobial samples is hampered by fast-growing microorganisms. Malassezia selective culture media are needed because Malassezia are resistant to cycloheximide, but some fungi, including the chief human commensal Candida albicans resist to this compound. This study aimed to test whether the macrolide rapamycin could be used in combination with cycloheximide to develop a Malassezia-selective culture medium. Rapamycin susceptibility testing was performed via microdilution assays in modified Dixon against M. furfur and five Candida spp. The MIC was the lowest concentration producing 90% growth inhibition. Rapamycin medium ± cycloheximide 500 mg/L was also added to FastFung solid and yeast suspensions were inoculated and incubated for 72h. Rapamycin MICs against Candida spp. ranged from 0.5 to 2 mg/L, except for C. krusei whose MIC was >32 mg/L. M. furfur stains were rapamycin resistant. Rapamycin and cycloheximide supplementation of the FastFung medium effectively inhibited the growth of non-Malassezia yeast, including the cycloheximide-resistant C. albicans and C. tropicalis. Based on our findings, we recommend using this “MalaSelect” medium for Malassezia isolation and culture from polymicrobial samples.

The genus Naegleria, of free-living amoeba (FLA) group, has been investigated mainly due to its human health impact resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the “brain-eating amoeba”, is the most studied Naegleria species because it causes Primary Amoebic Meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation we present a research on identifying Naegleria spp. in the Monjolinho River, as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos. Five distinct sampling sites were examined through limnological features, trophozoites culturing and PCR against internal transcribed spacers (ITS) regions and 5.8S rRNA sequence. The results identified N. philippinensis, N. canariensisi, N. australiensis, N.gruberi, N. dobsoni sequences, as well as a Vahlkampfia sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our result stresses the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil.

The population of Bifidobacterium spp in faecal samples from breast-fed and formula-fed infants were investigated and developed antibiogram. The common bacterial growth media such as Man Rogos Sharpe (MRS), Brain Heart Infusion (BHI), Luria Bertani (LB) broth and Bifidobacteria agar were evaluated by the enumeration of Bifidobacterium spp. in faecal material. The results demonstrated that the population of Bifidobacterium spp. in the faecal samples of breast-fed infants is numerically high and, low in formula-fed infants. Phylogenetic analysis of the 16S rRNA, (Xylose/Fructose-6-phosphate phosphoketolase) xfp, and RFLP map of Bifidobacteria isolates suggested a presence of possible new and different Bifidobacterium spp. The intensity of the reddish brown colour formed during the f6ppk (Fructose-6-phosphate phosphoketolase) assay confirms the presence of variable Bifidobacteria. The highest recovery and diversity of Bifidobacteria were obtained from Bifidobacteria agar media. Growth in various media resulted in the formation of small (SCV) and big colony variants (BCV). We understand that the various antibiotic minimum inhibitory concentration (MIC) values obtained by applying different media, growth conditions, after bile salt treatment, and at low pH can vary. This study concludes that the variation of microbiological conditions differentiating resistant and susceptible strains is influenced by the test conditions as well.

The soil and groundwater of Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic contaminated. A bacterium was isolated from the garden soil of Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III), which makes it arsenic (III & V) and chromium (III) hyper-tolerant bacterium. The growth pattern of this bacterium does not show much alteration in presence of 10 mM arsenic (III) and chromium (III), emphasizing its resistance to these heavy metals. Scanning electron microscopy depicted the size of this bacterium to be ~1.45 µm. 16S rDNA sequencing followed by subsequent phylogenetic analysis established the identity of this bacterium as Microbacterium paraoxydans. The bacterium is capable of bioremediation of arsenic and showed 30.8% & 22.6%, and 35.2% and 30.5% of bioremediation over a period of 24 and 48 hours in 1 mM and 4 mM arsenite, respectively. Microbacterium paraoxydans also exhibit plant growth promoting properties like nitrogen fixation, phosphate solubilization, indole-3-acetic acid production and production of siderophores. Therefore, the heavy metal resistance, bioremediation potential and plant growth promoting potential of the bacterium could be utilized not only for reduction of arsenic toxicity in soil and groundwater but also for plant growth promotion by using it as a biofertilizer.

The thick functionally graded material (FGM) circular cylindrical shells with advanced varied shear correction coefficient and third-order shear deformation theory (TSDT) under advanced thermal vibration are studied by the method of generalized differential quadrature (GDQ). The coefficient of displacement model of TSDT is applied to derive the equations of motion for the thick FGM circular cylindrical shells. The stiffness in simpler forms of thick FGM circular cylindrical shells and temperature rise in linear expression of the heat conduction equation are used. The differential equations in dynamic equilibrium state of thick FGM circular cylindrical shells can be obtained and rewritten into displacements and shear rotations in partial derivative expressions under dynamic thermal loads in partial derivative expressions. Parametric effect studies including advanced nonlinear varied shear correction coefficient, nonlinear coefficient c1 value, power law index and thermal temperature difference of external heating loads on the displacements and stresses of thick FGM cylindrical shells under thermal dynamic vibration are investigated.

The cutting of difficult-to-machine materials, including their preconditioning, often requires specialised tools and machinery. An alternative processing technology can be cutting materials with a water jet. From the initial stage, the selection of appropriate technological parameters will influence what further finishing treatments will be applied. Vibrations occurring during this process can negatively affect the quality of the cutting edges and surfaces. Therefore, this study analysed vibrations during water-jet cutting with variable technological parameters (speed vfi and pressure pi). To measure the vibrations, a three-axis accelerometer from SEQUOIA was used in water-jet cutting of three materials: aluminum alloy, titanium alloy, and steel. Based on the study, it was found that the maximum value of vibration amplitude reaches the lowest value of vibration acceleration for aluminum alloy (not exceeding 5 m/s2), regardless of the value of pressure during the cutting process. This, taking into account the material properties, indicates that aluminum alloy is the material most susceptible to the cutting process while maintaining a high quality of the cutting surface. Significantly higher values of vibration acceleration amplitude (reaching up to 60 m/s2) during cutting were registered for steel and titanium alloy in all zones and phases of the process (entry zone, cutting zone, and exit zones).

This paper presents a study on the idle vibration reduction of a diesel sport utility vehicle (SUV). To reduce idle vibration, the transmission paths of vibration from the engine to the driver seat floor were investigated with the vehicle components related to idle vibration. Furthermore, operational deflection shape (ODS) tests were conducted to visualize the vibration shapes during engine idling. Experimental modal analyses were performed to obtain the natural frequencies and mode shapes. Through the ODS and modal tests, the vibration characteristics of the diesel SUV during idling were identified. Considering these vibration characteristics, a multi-body dynamic model for the diesel SUV described by differential equations of motion was established to evaluate the idle vibration. To implement the dynamic model effectively, the equivalent stiffnesses and damping coefficients included in the model were determined experimentally or analytically. The established dynamic model was verified by comparing the natural frequencies and idle vibration levels between simulations. Using this dynamic model, we analyzed the effects of various design variables on idle vibration and obtained an optimal design for reducing the idle vibration level. Finally, we present a design guide to reduce the idle vibration for diesel SUVs.

This paper proposes a novel data processing framework dedicated to bedload monitoring in underwater environments. After calibration, by integration the total energy in the nominal bandwidth, the proposed experimental setup is able to accurately measure the mass of individual sediments hitting the aluminum plate. This require a priori knowledge of the vibration trasients in order to match a predefined dictionary. Based on unsupervised hierarchical agglomeration of complex vibration spectra, the proposed algorithms allow to accurately localize the transients corresponding to the shocks created by sediment impacts on a steel plate.

Toxoplasma gondii is a widespread protozoan parasite that infects most warm-blooded animals, and felids can serve as intermediate and definitive hosts. Pathological diagnosis and serological and etiological investigations were conducted on a captive caracal (Caracal caracal) carcass collected from China in 2022. Pathological diagnosis revealed that cardiac insufficiency, pulmonary edema, hepatic failure, and renal insufficiency were the causes of the caracal’s death. A modified agglutination test (cut-off: 1:25) revealed that IgG antibodies against T. gondii were present in the myocardium juice, hydroperitoneum, and hydropericardium (≥1:800). A viable T. gondii (TgCaracalCHn2) strain was isolated from the tissue samples (heart, brain, spleen, and skeletal muscle) of this caracal using a mouse bioassay. The genotype of TgCaracalCHn2 was ToxoDB#5 (Type II variant), as determined by RFLP-PCR. The strain was avirulent in Swiss mice and matched the prediction of ROP18 and ROP5 gene alleles of TgCaracalCHn2 (2/2). Many tissue cysts (203 ± 265) were observed in mice brains after inoculation with TgCaracalCHn2 tachyzoites. ToxoDB#5 is the dominant genotype in North American wildlife, and this is the first documented isolation of T. gondii ToxoDB#5 from China. This indicates that caracals can accelerate the transmission of T. gondii genotypes.

Extracellular vesicles (EVs) are enclosed by a lipid-bilayer membrane and secreted by all types of the cells under various physiological conditions. EVs play important roles in intercellular communication and crosstalk between tissues in the body. They are classified into three groups, such as apoptotic bodies, microvesicles, and exosomes. Exosomes were isolated from biofluids including blood, urine, milk, and cell culture media. Exosomes have significant potential for drug delivery and diagnosis. However, the method of isolation affects the physical and biological properties of exosomes. Several methods based on different principles have been developed for exosome isolation. These include ultrafiltration, precipitation, ultracentrifugation, size-exclusion chromatography, and microfluidics. In this study, we applied three common methods, such as ultrafiltration, precipitation, and ultracentrifugation, to isolate exosomes from the cell culture medium and investigated the effects of these different isolation methods on the size distribution and quality of the isolated exosomes. Field emission scanning electron microscopy (FESEM) images, size distribution, total protein content, and the effect of exosomes on the viability of hypoxic cells were analyzed in this study. The analysis revealed that compared to other methods, the ultracentrifugation method can isolate exosomes with smaller diameter (ranging from 20 to 80 nm), lower total protein content (50 µg/ml), and causing the increased viability of the hypoxic cells. The precipitation method does not require special equipment and is inexpensive, if the quality and purity of this method are solved, and it can be used as the best method for exosome isolation. This study can serve as a guide for choosing the best exosome isolation method for applications in medicine according to the needs, time, cost, and equipment.

Fermented milk product "Laban" in Libya is one of the most a traditional fermented milk product consumed a refreshing drink, particularly in the warm season The average values of the physicochemical including titratable acidity, pH, total solids, and fat were 0.73%, 4.16, 8.12%, and 1.54% respectively. Coliform, yeast and mold counts were 21×10⁴, 39×10⁴, and 41 ×10³ cfu/ ml., respectively. Most strains of coliform bacteria were Serratia odorifera, Escherichia coli 1, E. coli 2. and Klebsiella oxytoca. The average Lactococcus, Streptococcus, Mesophilic Lactobacillus / Leuconostoc and Thermophilic Lactobacillus counts were 99 ×10⁷, 96 ×10⁷, 93 ×10⁷ and 15 ×10⁷ cfu / ml. respectively. A total of 142 lactic acid bacteria (LAB) isolates were identified to the genus level as Lactobacillus (48.59%), Lactococcus (43.66%), Streptococcus (4.93%) and Leuconostoc (2.82%). Sugar fermentation tests revealed the most frequent Lactobacillus species found to be Lactobacillus delbrueckii ssp. lactis (62.32%), followed by Lactobacillus plantarum (31.88%). Furthermore, other selected LAB isolates were identified by API 50 CH test as Lactococcus lactis ssp. lactics, Lactobacillus pentosus, Lactobacillus brevis, and Leuconostoc mesenteroides ssp. cremoris. Thus, our research documented the lactic acid bacteria strains and will provides fundamental basic and useful information for further studies of strain selection starter culture, with regard to the industrial production of fermented dairy milk products.

Bacterial Calcium-carbonate Precipitation (BCP) has been studied for multiple applications such as remediation, consolidation and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper we studied CaCO₃ precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria and Alphaproteobacteria are the dominant bacteria involved in CaCO₃ precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO₃, mostly in the form of calcite. In this study Biolog® Eco-plate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO₃ producer strains.

This paper exposes the initial part of a larger research on the impact of the automotive on human health and the quality of life and environment. After a brief introduction on the need for responsible and sustainable approaches and few questions on the subject, some considerations and statistical data are presented, based on a study of the literature on the impact of road traffic and vehicles noise and vibration on people's comfort and health and their quality of life. The research results show, based on official statistics, the significant harmful impact of noise and vibration from motor vehicles and road transport on people's health and quality of life, especially in urban areas during the day. Also, the significant increase in the number of electric and hybrid vehicles from one year to another, a reality and a necessity nowadays, and the awareness that electric vehicles are not perfectly quiet and comfortable, open new research opportunities and require the development of new standards, materials, tools, equipment and test methods in the field of NVH, in a sustained synergistic approach from all stakeholders, to meet the needs and demands of today's consumers, and to comply with existing regulations and standards on environmental protection and sustainable development.

Six mode shapes, including bending and torsion, were documented for five different basketball rims and backboards at the United States Military Academy, West Point, New York, USA. The frequency and damping ratio of each mode shape were also determined. The empirical process began with the time-domain excitation and response of each rim-backboard system. The impulse of excitation came from an impact hammer separately applied to sequentially, to each node. The sinusoidal response was gathered from an accelerometer at a fixed location, node 1. Each time-domain excitation-response was then converted to a frequency domain Bode plot for each node by a B&K 2034 Signal Analyzer, giving transfer functions of output/input versus frequency. Structural Measurements System (SMS) Software was used to fit mode shapes to the Bode plots. Each of the six mode shapes were fitted to the Bode plots of each node at a specific modal frequency. Each of the six mode shapes were a function of the locations of the nodes, and the Bode plot gathered at each node. The first and second modes were critical for showing that the Energy Rebound Testing Device statistically correlated with the energy transferred to the rim and backboard. A known perturbation mass was selectively attached to the rim, to help isolate the dynamic masses and spring rates for the rim and backboard, to ascertain the kinetic energy transferred to the rim had a 95.67% inverse correlation with rim stiffness.

The present study investigates the role of a simple inerter in supplemental devices for possible implementation in the matured seismic base isolation technique. Firstly, the response of the base-isolated structure with an optimally tuned mass damper-inerter (TMDI) is investigated to see the tuning effects. The time required to tune the TMDI was found to be significantly longer than the duration of a strong-motion earthquake. There was still a reduction in the response of the isolated structure, which is primarily due to the added damping and stiffness (ADAS) of TMDI and not because of the tuning effects. Hence, it is proposed that the corresponding ADAS of the TMDI be directly added to the isolation device. Secondly, the response of the base-isolated structures with the fluid inerter damper (FID) is studied. It was observed that the inerter of the FID does not influence the displacement variance of an isolated structure under broadband earthquake excitation. It implies that the response of the isolated structure with FID is primarily controlled by its counterpart fluid damper (FD). The performance of optimal TMDI, ADAS, FID, and FD to mitigate the seismic response of the flexible multi-story base-isolated structure under real earthquake excitations is also investigated. In terms of suppressing the displacement and acceleration responses of the isolated structure, it has been found that TMDI and ADAS perform similarly. Comparing the response of the isolated structure with FID and FD demonstrated that the inerter in the FID has detrimental effects on the isolated structures, in which the top floor's acceleration and base shear are substantially increased.

Reproductive isolating barriers are a crucial element in the speciation process. There are various types of barriers, and within those that act early in the speciation process, the behavioral one can play an important role in isolating populations or closely related species. It has been shown that the high diversity of some taxa correlates with the variation of sexual signals, which facilitate isolation. Here it is explored whether chemical signals may contribute to the high diversity of Liolaemus lizards, which has more than 290 species. Population chemical discrimination was investigated in two co-distributed species, L. tenuis and L. lemniscatus. The studied populations were closely located and likely belonged to the same genetic lineages. Lizards of both species discriminated between scents of their own and different populations, and in most cases, scents from their population were preferred. Results suggest a fast evolution of scents, and their recognition, and there for the ethological barriers associated with the chemical modality may evolve fast. However, because the studied species differed in the strength of the exhibited population chemical discrimination, ethological barriers evolve at different rates across species. It can be concluded that ethological barriers involving the chemical modality may disrupt species' cohesion, contributing to Liolaemus diversification, in synergy or not with geographical barriers.

Microalgae are known as a rich source of bioactive compounds which exhibit different biological activities. Increased demand for sustainable biomass for production of important bioactive components with various potential especially therapeutic applications has resulted in noticeable interest in algae. Utilization of microalgae in multiple scopes has been growing in various industries ranging from harnessing renewable energy to exploitation of high-value products. The focuses of this review are on the isolation of bioactive compounds from microalgae regarding different metabolites which are currently used and new possible applications of the compounds in industries and future prospects. Moreover, this work discusses the advantage, potential new beneficial strains, applications, limitations, research gaps and future prospect of microalgae in industry.

The study aimed to isolate and characterize bacteriocin-producing Enterococcus species from Ethiopian traditional fermented foods and beverages. The bacteriocin bioassay was tested against food-borne pathogens at various temperatures, pH, solvents, additives, and enzymes. A solvent extraction method was established to concentrate bacteriocins from Enterococcus species. The study found that crude extracts with proteinase K, pepsin, and trypsin deactivated antimicrobial activity, confirming their proteinaceous nature. Organic solvents were more effective at extracting bacteriocins from CFS, with chloroform alone and in combination providing rapid and efficient recovery. The optimal bacteriocin production and strong inhibition activity were achieved at 37°C with a pH range of 5.5 to 6.5 incubated for 16-18 hours. The six Enterococcus faecium species were the best bacteriocin-producing isolates. Optimizing bacteriocin production and changing environmental growing circumstances can enhance the application of bacteriocins as food preservation agents. Traditional fermented products are potential sources of bacteriocin-producing LAB.

Investigation of the methanol extract of the poroid fungus Fuscoporia torulosa resulted in the isolation of a novel triterpene, fuscoporic acid (1) together with inoscavin A and its previously undescribed Z isomer (2 and 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic acid (6), natalic acid (7), and ergosta-7,22-diene-3-one (8). The structures of fungal compounds were determined on the basis of NMR and MS spectroscopic analysis, as well as molecular modelling studies. Compounds 1, 6-8 were examined for their antibacterial properties on resistant clinical isolates, and cytotoxic activity on human colon adenocarcinoma cell lines. Compound 8 was effective against Colo 205 (IC50 11.65±1.67 µM), Colo 320 (IC50 8.43±1.1 µM) and MRC-5 (IC50 7.92±1.42 µM) cell lines. Potentially synergistic relationship was investigated between 8 and doxorubicin, which revealed a synergism between the examined compounds with a combination index (CI) at the 50% growth inhibition dose (ED50) of 0.521±0.15. Several compounds (1, and 6-8) were tested for P‐glycoprotein modulatory effect in Colo 320 resistant cancer cells, but none of the compounds proved to be effective in this assay. Fungal metabolites 2-5 were evaluated for their antioxidant activity using the oxygen radical absorbance capacity (ORAC) and DPPH assays. Compounds 4 and 5 proved to possess considerable antioxidant effect with EC50 0.25±0.01 (DPPH) and 12.20±0.92 mmol TE/g (ORAC). The current article provides valuable information on both chemical and pharmacological profiles of Fuscoporia torulosa, paving the way for future studies with this species.

Machinery parts gradually wear out over time due to regular usage. To improve machinery health and prevent critical issues, a reliable prognosis framework can be implemented by monitoring the behaviour of machinery parts and issuing warnings before they reach a critical state. To achieve this, vibration data from roller bearings experiencing various fault conditions have been collected. Different techniques from the literature were combined to analyze the distinct configurations in the vibration data sets and identify the main defects in roller bearings. The significant features extracted from this analysis were then used to create optimized stochastic model equations, separately regressing inner and outer race fault features to healthy bearing features under random conditions. These models can help engineers design more dependable systems, optimize their performance, and minimize the risk of failures and downtime.

The paper presents the results of a study of the concept of address fiber Bragg structures in the problem of vibration control. The mathematical model of measuring transformation is presented; the experimental study of a vibration diagnostics system based on Address Fiber Bragg Gratings is carried out; the quantitative and qualitative comparative assessment with electronic accelerometers is made; the gain by an order of magnitude in some parameters is shown.

Complex blade responses such as a rotating stall or simultaneous resonances are common in modern engines and their observation can be a challenge even for state-of-the-art tip-timing systems and trained operators. This paper analyses forced vibrations of axial compressor blades, measured during the bench tests of the SO-3 turbojet. In relation to earlier studies conducted in ITWL with a small number of sensors, a multichannel tip-timing system let us observe simultaneous responses or higher-order modes. To find possible symptoms of a failure, blade responses in a healthy and unhealthy engine configuration with an inlet blocker were studied. The used analysis methods covered all-blade spectrum and the circumferential fitting of blade deflections to the harmonic oscillator model. The proposed modal solver can track the vibration frequency and adjust the engine order on the fly. That way, synchronous and asynchronous vibrations are observed and analysed together with an extended variant of least squares. The proposed approach helps to avoid common mistakes and saves a lot of work related to configuring the conventional solver.

In a software-defined radar (SDR) system, most of the signal processing usually implemented in hardware is implemented by software, thus allowing for higher flexibility and modularity compared to conventional radar systems. However, the majority of SDR demonstrators and proofs-of-concept reported in the open literature so far have been based on simple antenna systems. As a result, the full potentialities of an SDR approach have not been completely exploited yet. In this work, we propose a flexilbe antenna module to be integrated into an active electronically scanning array (AESA) with controlled sidelobe level over a wide angular range, exhibiting polarization reconfigurability with low cross-polarization level and high isolation. For this purpose, analytical and numerically efficient techniques for the synthesis of the aperture distribution and the correct evaluation of the radiating features (e.g., beamwidth, pointing angle, sidelobe levels, etc.) are presented in order to grant a real-time control of the digital beamforming network. A sub-array module demonstrator is fabricated and measured to corroborate the concept.

Agrobacterium tumefaciens (A. tumefaciens) is a plant pathogenic bacterium that causes crown gall disease, leading to significant economic losses in various agriculturally important crops, including apple, pear, peach, and almond. The difficulty of controlling A. tumefaciens with conventional pesticides underscores the need for alternative antibacterial agents such as bacteriophages. In this study, a novel lytic bacteriophage, named Agrobacterium phage PAT1 (PAT1), with high lysis potential against A. tumefaciens, was isolated from wastewater. The interaction between PAT1 and A. tumefaciens cells, investigated using Transmission electron microscopy, revealed that PAT1 efficiently adsorb, infect, and replicate on A. tumefaciens in a very short time period (i.e., ≤30 min for a complete infectious cycle). Furthermore, the turbidity assay showed that PAT1 (MOI = 1) exerted a significant inhibitory effect on A. tumefaciens for 48 hours, resulting in an 82% reduction in bacterial growth. Additionally, stability tests showed that PAT1 was highly resistant to a broad range of pH (4-10) and temperatures (4-60°C). Bioinformatics analyses of the genomic sequence suggested that PAT1 is a new phage closely related to Agrobacterium phages of the genus Atuphduovirus within the family Autographiviridae. The genome size was 45,040 base pairs with a G+C content of 54.5%, consisting of 54 coding sequences (CDS), of which the functions of 22 CDS were predicted, including two endolysins genes which could be used as antimicrobials against A. tumefaciens. Additionally, no lysogenic mediated genes or genes encoding virulence factors, antibiotic resistance, or toxins were detected in PAT1 genome. Overall, PAT1 demonstrated suitability as a potential biocontrol agent for combating A. tumefaciens infections, expanding the limited catalog of such lytic A. tumefaciens phages. However, its efficacy in controlling crown gall disease in planta remains to be evaluated.