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A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.

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  • 1. Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, MD.
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    • McDaniel TK 1
    (1 author)

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Proceedings of the National Academy of Sciences of the United States of America, 01 Feb 1995, 92(5):1664-1668
https://doi.org/10.1073/pnas.92.5.1664 PMID: 7878036 PMCID: PMC42580

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Abstract 

Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 are intestinal pathogens that profoundly damage the microvilli and subapical cytoskeleton of epithelial cells. Here we report finding in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions. DNA probes throughout this locus hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridize to avirulent members of the same species. The EPEC locus and a different virulence locus of uropathogenic E. coli insert into the E. coli chromosome at the identical site and share highly similar sequences near the point of insertion.

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Proc Natl Acad Sci U S A. 1995 Feb 28; 92(5): 1664–1668.
PMCID: PMC42580
PMID: 7878036

A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.

T K McDaniel, K G Jarvis, M S Donnenberg, and J B Kaper
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Abstract

Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 are intestinal pathogens that profoundly damage the microvilli and subapical cytoskeleton of epithelial cells. Here we report finding in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions. DNA probes throughout this locus hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridize to avirulent members of the same species. The EPEC locus and a different virulence locus of uropathogenic E. coli insert into the E. coli chromosome at the identical site and share highly similar sequences near the point of insertion.

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Selected References

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