|
| Status |
Public on Nov 21, 2016 |
| Title |
Cecal tissue_FRdiet_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cecal tissue, Fiber rich diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss webster
diet: fiber-rich diet
tissue: cecum
|
| Growth protocol |
Mice were fed either a diet rich in fiber (control) or a diet lacking fiber (experimental condition)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bead beating and Trizol extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2) from 400 ng total RNA
|
| |
|
| Hybridization protocol |
Following labeling procedure, 2.76 ug of cDNA were hybridized at 48C on Mouse Gene ST 2.1 strips, and were washed and stained using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Description |
Martens_001_MD32_FR
|
| Data processing |
Expression values for each gene were calculated using robust multi-array average (RMA) method
|
| |
|
| Submission date |
Mar 10, 2016 |
| Last update date |
Nov 21, 2016 |
| Contact name |
Eric Charles Martens |
| E-mail(s) |
[email protected]
|
| Phone |
734-647-5800
|
| Organization name |
University of Michigan Medical School
|
| Department |
Dept. of Microbiology and Immunology
|
| Street address |
1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE79067 |
To determine transcriptome of gnotobiotic mice fed fiber-rich and fiber-free diets |
|
