How can I query UniProtKB annotations by evidence?
The table below summarizes the different evidence types used in UniProtKB annotation, and details how they are labeled in the Advanced search menu and in the UniProtKB entry view.
| Advanced search | ECO_0000XXX | Source | Entry view labels | Notes | Experimental | Manual | Automatic |
| Manual assertions (in UniProtKB/Swiss-Prot) | |||||||
| Experimental | ECO_0000269 | Literature reference | x Publication(s) with "Manual assertion based on experiment in" | yes | yes | no | |
| Non-traceable author statement | ECO_0000303 | Literature reference | x Publication(s) with "Manual assertion based on opinion in" | yes | yes | no | |
| Curator inference | ECO_0000305 | Literature reference (optional) | Curated, x Publication(s) with "Manual assertion inferred by curator from" | no | yes | no | |
| Sequence similarity | ECO_0000250 | Another UniProtKB/Swiss-Prot entry | By similarity | no | yes | no | |
| Sequence model | ECO_0000255 | A rule (optional) | Sequence analysis, UniRule annotation, Prosite ProRule annotation, SAAS annotation | no | yes | no | |
| Combinatorial | ECO_0000244 | a PDB entry or literature reference (for large-scale proteomics publications) | Combined sources | no | yes | no | |
| Imported information | ECO_0000312 | an entry from nucleotide sequence databases, PDB, model organism databases | Imported | no | yes | no | |
| Automatic assertions (in UniProtKB/TrEMBL) | |||||||
| Sequence model | ECO_0000256 | a sequence analysis program or an automatic annotation rule | Sequence analysis (SIGNAL, TRANSMEM, COILED or MobiDB-lite), UniRule annotation (RuleBase, HAMAP or PIRNR/PIRSR), SAAS annotation | no | no | yes | |
| Combinatorial | ECO_0000213 | A PDB entry | Combined sources | no | no | yes | |
| Imported information | ECO_0000313 | An entry from nucleotide sequence databases (EMBL, Ensembl), PDB, model organism databases | Imported | no | no | yes | |
| Sequence motif match (InterPro) | ECO_0000259 | An entry in an InterPro member database | InterPro annotation | used for domain annotation | no | no | yes |
