Abstract
We identified a viral IL-10 homolog encoded by an ORF (UL111a) within the human cytomegalovirus (CMV) genome, which we designated cmvIL-10. cmvIL-10 can bind to the human IL-10 receptor and can compete with human IL-10 for binding sites, despite the fact that these two proteins are only 27% identical. cmvIL-10 requires both subunits of the IL-10 receptor complex to induce signal transduction events and biological activities. The structure of the cmvIL-10 gene is unique by itself. The gene retained two of four introns of the IL-10 gene, but the length of the introns was reduced. We demonstrated that cmvIL-10 is expressed in CMV-infected cells. Thus, expression of cmvIL-10 extends the range of counter measures developed by CMV to circumvent detection and destruction by the host immune system.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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Cytomegalovirus / chemistry
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Cytomegalovirus / genetics*
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DNA-Binding Proteins / metabolism
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Genes, Viral / genetics
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Genome, Viral
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Growth Substances / chemistry
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Growth Substances / genetics*
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Humans
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Interleukin-10 / chemistry
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Interleukin-10 / genetics*
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Leukocytes
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Major Histocompatibility Complex / genetics
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Molecular Sequence Data
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Protein Binding
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Receptors, Interleukin / metabolism
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Receptors, Interleukin-10
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STAT3 Transcription Factor
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Sequence Alignment
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Signal Transduction
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Trans-Activators / metabolism
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Transfection
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Viral Proteins / chemistry
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Viral Proteins / genetics*
Substances
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DNA-Binding Proteins
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Growth Substances
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IL-10 like protein, Human herpesvirus 5
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Receptors, Interleukin
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Receptors, Interleukin-10
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STAT3 Transcription Factor
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STAT3 protein, human
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Trans-Activators
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Viral Proteins
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Interleukin-10