Abstract
Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
MeSH terms
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Administration, Oral
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Animals
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Antibodies, Viral / blood
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Antigens, Viral / administration & dosage
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Coronavirus / chemistry
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Coronavirus / genetics
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Coronavirus / immunology*
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Coronavirus Infections / prevention & control
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Enzyme-Linked Immunosorbent Assay
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology*
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Mice
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Mice, Inbred BALB C
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Plant Proteins / genetics
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Plants, Genetically Modified
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Plasmids
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Polymerase Chain Reaction
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Recombinant Proteins / administration & dosage
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Solanum tuberosum*
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Spike Glycoprotein, Coronavirus
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Transformation, Genetic
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Vaccines, Synthetic / administration & dosage
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Vaccines / administration & dosage
Substances
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Antibodies, Viral
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Antigens, Viral
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Membrane Glycoproteins
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Plant Proteins
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Recombinant Proteins
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Spike Glycoprotein, Coronavirus
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Vaccines