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Use of commercial enzyme immunoassays to detect antibodies to the herpes simplex virus type 2 glycoprotein G in a low-risk population in Hanoi, Vietnam

Clin Vaccine Immunol. 2008 Feb;15(2):382-4. doi: 10.1128/CVI.00437-06. Epub 2007 Dec 12.

Abstract

Sera from 1,238 Vietnamese women in Hanoi were tested for herpes simplex virus type 2 (HSV-2). HSV-2 prevalence was 2.0%. The Kalon and Biokit assays showed significantly higher concordance to Western blotting data than did the Focus assay (P < 0.01). Screening by Focus and then retesting with Kalon/Biokit of positive samples can reduce falsely positive results significantly (P < 0.01).

MeSH terms

  • Africa, Western
  • Antibodies, Viral / blood*
  • False Positive Reactions
  • Female
  • Herpes Simplex / diagnosis*
  • Herpes Simplex / epidemiology
  • Herpes Simplex / virology
  • Herpesvirus 2, Human / immunology
  • Humans
  • Immunoenzyme Techniques / methods*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Seroepidemiologic Studies
  • Vietnam / epidemiology
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Viral
  • Reagent Kits, Diagnostic
  • Viral Envelope Proteins
  • glycoprotein G, herpes simplex virus type 2