Pch2 Links Chromosome Axis Remodeling at Future Crossover Sites and Crossover Distribution during Yeast Meiosis
Figure 1
Association of Pch2 with WT chromosomes at different stages of SC polymerization.
(A–H) Early zygotene nuclei; Zip1 (A,E), and HA-Pch2 (B,F). (I–L) Late zygotene nucleus. (M–P) Pachytene nucleus. Arrows indicate rDNA clusters, identified by Pch2 localization pattern and lack of Zip1 (K,O). Spread zygotene and pachytene nuclei are identified base on extend of Zip1 staining. Colors are indicated by the corresponding labels. Meiosis was induced at 33°C.