Gamma-Tubulin Is Required for Bipolar Spindle Assembly and for Proper Kinetochore Microtubule Attachments during Prometaphase I in Drosophila Oocytes
Figure 7
The spindle pole component D-TACC is mislocalized in γtub37CP162L mutant oocytes.
Oocytes were immuno-stained to D-TACC (green), α-tubulin (red) and DAPI (blue). (A) A wild-type prometaphase I spindle with polar and diffuse D-TACC localization. (B) A wild-type metaphase I spindle with D-TACC staining at the spindle poles and diffuse staining along the microtubules. (C) Large, bright patches of D-TACC cover part of the spindle while D-TACC is absent in other regions in a prometaphase I γtub37CP162L mutant oocyte. (D) Small puncta of D-TACC are present near the DNA, but D-TACC is extremely weak or absent near the poles in a prometaphase I γtub37CP162L mutant oocyte. (E) D-TACC is absent or extremely weak from the spindle in a prometaphase I γtub37CP162L mutant oocyte despite strong localization of D-TACC to cytoplasmic structures. (F) A metaphase I spindle from a γtub37CP162L mutant oocyte lacking clear D-TACC staining. Shown are single Z slices since projection of the entire Z stack results in images with high background in the D-TACC channel. For the examples of γtub37CP162L mutant oocytes, spindles that displayed directionality were analyzed to rule out D-TACC mislocalization being caused by lack of a clear spindle. Scale bars are in microns.