Mammalian Neurogenesis Requires Treacle-Plk1 for Precise Control of Spindle Orientation, Mitotic Progression, and Maintenance of Neural Progenitor Cells
Figure 5
Dynamic localization of Treacle in mitotic cells.
(A) Immunofluorescence images of wild-type forebrain at E11.5 detecting Treacle (red), the centrosome (γ-tubulin; green) and nuclei (DAPI; blue). Arrowheads and arrows indicate Treacle localization at the centrosome in interphase cells and mitotic cells respectively. (B) Immunofluorescence images of HeLa cells showing the dynamic localization of Treacle (green) during mitosis, particularly at the centrosomes (arrowheads) and kinetochore in prophase, prometaphase and metaphase cells as well as at the midzone during anaphase (arrow) and at the midbody (arrow) in telophase. The microtubular networks are detected by immunostaining with an anti-α-tubulin antibody. (C) Treacle co-localizes with the kinetochore marker, CENP-E. Scale Bars: A and B, 10 µm.