Transcriptional Control in Cardiac Progenitors: Tbx1 Interacts with the BAF Chromatin Remodeling Complex and Regulates Wnt5a
Figure 5
β-Catenin is upregulated in the SHF of Tbx1−/−;Wnt5a−/− embryos at E9.5.
(A–D) Immunohistochemistry using an anti-cleaved Caspase 3 antibody showed no obvious difference in apoptosis between Wnt5a−/− and Tbx1−/−; Wnt5a−/− embryos at E9.5. (E–K) Immunofluorescence using a β-catenin antibody showed no change of expression in the SHF (arrows) of Tbx1−/− or Wnt5a−/− embryos (E–G), while it was strongly upregulated in the SHF of Tbx1−/−; Wnt5a−/− embryos at E9.5 (H–K panels from independent embryos). Squared regions are magnified in the bottom row of panels (E′–K′). Red: β-catenin; Blue: DAPI. PE: pharyngeal endoderm; OFT: outflow tract; RA: right atrium; Scale bars: 100 µm.