AMPK signaling linked to the schizophrenia-associated 1q21.1 deletion is required for neuronal and sleep maintenance
Fig 1
Loss of AMPK in the nervous system reduces overall lifespan.
(A) Survival graph showing overall survival for an 89-day period, for male flies raised and kept on standard food. Results obtained from 10 replicates per genotype, each containing 30 flies. (B) Quantification of overall survival. Knockdown of alc or overexpression of AMPKα-DN in the nervous system reduces overall longevity. Survival analyzed using Kaplan-Meier nonparametric method. Survival quantified by fitting each replicate by a Weibull distribution function, right-censoring animals alive at end of experiment. (C) Survival graph showing overall survival for an 80-hour period, for 3-5-day-old male flies raised on normal food and starved on 2% agar alone. Results were obtained from 9–10 replicates per genotype, each containing 30 flies (n > 270). (D) Quantification of survival following starvation. Pan-neuronal knockdown of alc increases sensitivity to starvation. For controls, lines were crossed to w1118. Error bars indicate SEM. Kruskal-Wallis test with Dunn’s post-hoc testing was used to determine statistical significance: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, versus the control.