IL-33 Is Produced by Mast Cells and Regulates IgE-Dependent Inflammation
Figure 5
IL-33/ST2 pathway is critical for the development of IgE-driven tissue inflammation during anaphylaxis.
Induction of ear swelling during passive cutaneous anaphylaxis was determined after systemic antigen challenge in mice receiving no treatment, 10 µg anti-murine IL-33 antibody or 10 µg isotype control (A), anti-murine ST2 antibody or isotype control (B) or in ST2−/− or ST2−/+ control mice (C). Data represents the mean±SEM from 10–15 mice. The IL-33 protein levels in ear tissue from IgE injected or PBS injected sites were determined by ELISA during the PCA time course (D) (n = 3 mice per time point). Increases in IL-33 at IgE injected skin sites (determined as the delta between the PBS injected skin from the same individual) were determined in W/Wv, reconstituted W/Wv, ST2−/− or relevant control strains (n = 3–9 mice per group). * = p<0.05, ** = p<0.01, *** = p<0.005 by Students t-test.