Hydrophobin Fusion of an Influenza Virus Hemagglutinin Allows High Transient Expression in Nicotiana benthamiana, Easy Purification and Immune Response with Neutralizing Activity
Figure 2
Accumulation of HA fused or not to HFBI.
The H1 and H1-HFBI constructs were transiently expressed in N. benthamiana leaves. One construct was abaxially infiltrated on one half of the leaf and the other one on the other half. (a) Coomassie-blue stained gel of 30 µg TSP from four representative extracts. TSP from a plant infiltrated with an A. tumefaciens strain containing an empty vector was used as a negative control (C-). The bands corresponding to H1-HFBI and H1 are indicated by an arrow. (b) Western blot analysis of 5 µg TSP coming from the same extracts as in (a). The signal was detected with polyclonal anti-influenza A.