Hydrophobin Fusion of an Influenza Virus Hemagglutinin Allows High Transient Expression in Nicotiana benthamiana, Easy Purification and Immune Response with Neutralizing Activity
Figure 4
Endoglycosidase H treatment of H1-HFBI.
Time course incubation of a leaf TSP extract (5 µg) in the presence (+) or absence (−) of endoglycosidase H (0.2 U/ml). Samples were analyzed by Western blotting after 0, 15, or 60 min of incubation. The signal was detected with polyclonal anti-influenza A antibodies.