Svoboda | Graniru | BBC Russia | Golosameriki | Facebook

Plant Protect. Sci., 2021, 57(4):279-288 | DOI: 10.17221/39/2021-PPS

Virulence of Phytophthora infestans isolates from potato in SpainOriginal Paper

Alba Alvarez-Morezuelas, Nestor Alor, Leire Barandalla, Enrique Ritter, Jose Ignacio Ruiz de Galarreta ORCID...*
NEIKER-Basque Institute for Agricultural Research and Development-BRTA, Vitoria, Spain

The oomycete Phytophthora infestans is responsible for the disease known as late blight in potato and tomato. It is the plant pathogen that has caused the greatest impact on humankind so far and, despite all the studies that have been made, it remains the most important in this crop. In Spain during the last years a greater severity of the disease has been observed in both, potato and tomato, probably due to genetic changes in pathogen populations described recently. The aim of this study was the characterization of the physiological strains of 52 isolates of P. infestans obtained in different potato-growing areas in Spain. For this purpose, inoculations on detached leaves were performed in order to determine compatibility or incompatibility reactions. A total of 17 physiological races were found. The less frequent virulence factors were Avr5 and Avr8. By studying the epidemiology of the pathogen, a specific breeding program for late blight resistance can be implemented.

Keywords: physiological strains; late blight; Solanum tuberosum; virulence

Published: September 26, 2021  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Alvarez-Morezuelas A, Alor N, Barandalla L, Ritter E, Ruiz de Galarreta JI. Virulence of Phytophthora infestans isolates from potato in Spain. Plant Protect. Sci.. 2021;57(4):279-288. doi: 10.17221/39/2021-PPS.
Download citation

References

  1. Alor N., Gutiérrez I., Ruiz de Galarreta J.I. (2014): Prospección e identificación de aislados de Phytophthora infestans en el Norte de España. Revista Ciencia y Desarrollo, 17: 7-11. Go to original source...
  2. Alor N., Tierno R., Cooke D.E., Ruiz de Galarreta J.I. (2019): Characterisation of Phytophthora infestans isolates of potato crops from Spain. Potato Research, 62: 453-463. Go to original source...
  3. Andrivon D., Corbière R., Lebreton L., Pilet F., Montarry J., Pelle R., Ellisseche D. (2004): Host adaptation in Phytophthora infestans: A review from population biology perspectives. Plant Breeding and Seed Science, 50: 15-28.
  4. Andrivon D. (1994): Races of Phytophthora infestans in France, 1991-1993. Potato Reearch, 37: 279-286. Go to original source...
  5. Ballvora A., Ercolano M.R., Weiss J., Meksem K., Bormann C.A., Oberhagemann P., Salamini F., Gebhardt C. (2002): The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant Journal, 30: 361-371. Go to original source... Go to PubMed...
  6. Barquero M., Brenes A., Gómez L. (2005): Complejidad fisiológica de Phytophthora infestans en Costa Rica. Agronomía Costarricense, 29: 21-29.
  7. Beninal L., Corbière R., Kedad A., Andrivon D., Bouznad Z. (2009): A2 mating type, Metalaxyl resistance and complex virulence profiles: Common features in some Phytophthora infestans isolates from Algeria. In: Proc. 11th Workshop on Euroblight. Hamar, Oct 28-31, 2008: 237-241.
  8. Black W., Mastenbroek C., Mills W.R., Peterson L.C. (1953): A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica, 2: 173-179. Go to original source...
  9. Birch P.R., Whisson S.C. (2001): Phytophthora infestans enters the genomics era. Molecular Plant Pathology, 2: 257-263. Go to original source... Go to PubMed...
  10. Bradshaw J.E., Bryan G.J., Lees A.K., McLean K., SolomonBlackburn R.M. (2006): Mapping the R10 and R11 genes for resistance to late blight (Phytophthora infestans) present in the potato (Solanum tuberosum) R-gene differentials of black. Theoretical and Applied Genetics, 112: 744-751. Go to original source... Go to PubMed...
  11. Brylinska M., Sobkowiak S., Stefañczyk E., ¦liwka J. (2016): Potato cultivation system affects population structure of Phytophthora infestans. Fungal Ecology, 20: 132-143. Go to original source...
  12. Casa-Coila V.H., Gomes C.B., Lima-Medina I., Rocha D. J.A., Reis A. (2020): Characterization of mating type & the diversity of pathotypes of Phytophthora infestans isolates from Southern Brazil. Journal of Plant Diseases and Protection, 127: 43-54. Go to original source...
  13. Caten C.E., Jinks J.L. (1968): Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Canadian Journal of Botany, 46: 329-348. Go to original source...
  14. Cooke L.R., Schepers H.T.A.M., Hermansen A., Bain R.A., Bradshaw N.J., Ritchie F., Shaw D.S., Evenhuis A., Kessel G.J.T., Wander J.G.N., Andersson B., Hansen J.G., Hannukkala A., Naerstad R., Nielsen B.J. (2011): Epidemiology and integrated control of potato late blight in Europe. Potato Research, 54: 183-222. Go to original source...
  15. Dowley L.J., O'Sullivan E., Griffin D., Harmey M. (2000): Genetic Analysis of Irish Populations of Phytophthora infestans, End of Project Report, September 2000. Dublin, Teagasc.
  16. Forbes G.A., Escobar X.C., Ayala C.C., Revelo J., Ordoñez M.E., Fry B.A., Doucett K., Fry W.E. (1997): Population genetic structure of Phytophthtora infestans in Ecuador. Phytophatology, 87: 375-380. Go to original source... Go to PubMed...
  17. Fry W.E. (2008): Plant diseases that changed the world, Phytophthora infestans: The plant (and R gene) destroyer. Molecular and Plant Patholoy, 9: 385-402. Go to original source... Go to PubMed...
  18. Fry W.E., Drenth A., Spielman B.C., Mantel L., Davidse L.C., Goodwin S.B. (1991): Population genetic structure of Phytophthora infestans in the Netherlands. Phytopathology, 81: 1330-1336. Go to original source...
  19. Fukue Y., Akino S., Osawa H., Kondo N. (2018): Races of Phytophthora infestans isolated from potato in Hokkaido, Japan. Journal of General Plant Pathology, 84: 276-278. Go to original source...
  20. González M.L., Barrios S.E.G., Rovesti L., Palma R.S. (2006): Manejo Integrado de Plagas. Manual Práctico. La Habana, Centro Nacional de Sanidad Vegetal (CNSV).
  21. Goodwin S.B. (1997): The population genetics of Phytophthora. Phytopathology, 87: 462-437. Go to original source... Go to PubMed...
  22. Goodwin S.B., Sujkowski L.S., Fry W.E. (1995): Rapid evolution of pathogenicity within clonal lineages of the potato late blight disease fungus. Phytopathology, 85: 669-676. Go to original source...
  23. Goodwin S.B., Fry W.E. (1994): Genetic analyses of interspecific hybrids between Phytophthora infestans and Phytophthora mirabilis. Experimental Mycology, 18: 20-32. Go to original source...
  24. Goss E.M., Tabima J.F., Cooke D.E., Restrepo S., Fry W.E., Forbes G.A., Grünwald N.J. (2014): The Irish potato famine pathogen Phytophthora infestans originated in central Mexico rather than the Andes. Proceedings of the National Academy of Sciences, 111: 8791-8796. Go to original source... Go to PubMed...
  25. Grünwald N.J., Flier W.G. (2005): The biology of Phytophthora infestans at its center of origin. Annual Review of Phytopathology, 43: 171-190. Go to original source... Go to PubMed...
  26. Hannukkala A., Rastas M., Hannukkala A.E. (2008): Phenotypic characteristics of Finnish and North-Western Russian populations of Phytophthora infestans in 2006-2007. Proceedings of the Euroblight Workshop. Hamar, Oct 28-31, 2008: 191-195.
  27. Haverkort A., Struik P., Visser R., Jacobsen, E. (2009): Applied biotechnology to combat late blight in potato caused by Phytophthora infestans. Potato Research, 52: 249-264. Go to original source...
  28. Hein I., Birch P., Danan S., Lefebvre V., Achieng-Odeny D., Gebhardt C., Trognitz F., Bryan G. (2009): Progress in mapping and cloning qualitative and quantitative resistance against Phytophthora infestans in potato and its wild relatives. Potato Research, 52: 215-227. Go to original source...
  29. Huang S., Vleeshouwers V.G.A.A., Werij J.S., Hutten R.C.B., Van Eck H.J., Visser R.G.F., Jacobsen E. (2004): The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Molecular Plant-Microbe Interactions, 17: 428-435. Go to original source... Go to PubMed...
  30. Jaramillo S. (2003): Monograf ía Sobre Phytophthora infestans (Mont.) de Bary. Medellín, Universidad Nacional de Colombia.
  31. Lebecka R., Sliwka J., Sobkowiak S., Zimnoch-Guzowska E. (2007): Phytophthora infestans population in Poland. Proceedings of the 10th Workshop of an European Network for development of an integrated control strategy of potato late blight. Bologna, May 2-5, 2007: 155-159.
  32. Lebreton L., Andrivon D. (1998). French isolates of Phytophthora infestans from potato and tomato differ in phenotype and genotype. European Journal of Plant Pathology, 104: 583-594. Go to original source...
  33. Li G., Huang, S., Guo X., Li Y., Yang Y., Guo Z., Kuang H., Rietman H., Bergervoet M., Vleeshouwers V., Van der Vossen E.A.G., Qu D., Visser R., Jacobsen E., Vossen J. (2011): Cloning and characterization of R3b; members of the R3 superfamily of late blight resistance genes show sequence and functional divergence. Molecular Plant- Microbe Interaction, 24: 1132-1142. Go to original source... Go to PubMed...
  34. Malcolmson J.F. (1969): Races of Phytophthora infestans occurring in Great Britain. Transactions of the British Mycological Society, 53: 417-423. Go to original source...
  35. Malcolmson J.F., Black W. (1966): New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica, 15: 199-203. Go to original source...
  36. Matuszak J.M., Fernandez-Elquezabal J., Villarreal-Gonzalez M., Fry W.E. (1994): Sensitivity of Phytophthora infestans populations to metalaxyl in Mexico: Distribution and dynamics. Plant Disease, 78: 911-916. Go to original source...
  37. Michalska A.M., Sobkowiak S., Flis B., Zimnoch-Guzowska E. (2016). Virulence and aggressiveness of Phytophthora infestans isolates collected in Poland from potato and tomato plants identified no strong specificity. European Journal of Plant Pathology, 144: 325-336. Go to original source...
  38. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
  39. Oliva R.F., Erselius L.J., Adler N.E., Forbes G.A. (2002): Potential of sexual reproduction among host-adapted populations of Phytophthora infestans in Ecuador. Plant Pathology, 51: 710-719. Go to original source...
  40. Peters R.D., Plantt H.W., Hall R. (1998): Changes in race structure of Canadian populations of Phytophthora infestans based on specific virulence to selected potato clones. Potato Research, 41: 335-370. Go to original source...
  41. Runno-Paurson E., Kiiker R., Aav A., Hansen M., Williams I.H. (2006): Distribution of mating types, metalaxyl sensitivity and virulence races of Phytophthora infestans in Estonia. Agronomy Resarch, 14: 220-227.
  42. Schöber R., Turkensteen L.J. (1992): Recent and future developments in potato fungal pathology. Netherlands Journal of Plant Pathology, 98: 73-83. Go to original source...
  43. Swiezynski K.M., Domanski L., Zarzycka H., ZimnochGuzowska E. (2000): The reaction of potato differentials to Phytophthora infestans isolates collected in nature. Plant Breeding, 119: 119-126. Go to original source...
  44. Tooley P.W., Therrien C.D., Ritch D.L. (1989): Mating type, race composition, nuclear DNA cont and isozyme analysis of Peruvian isolates of Phytophthora infestans. Phytopathology, 79: 478-481. Go to original source...
  45. van Poppel P.M.J.A., Huigen D.J., Govers F. (2009): Differential recognition of Phytophthora infestans races in potato R4 breeding lines. Phytopathology, 99: 1150-1155. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.