Journal of the Serbian Chemical Society 2004 Volume 69, Issue 7, Pages: 571-574
https://doi.org/10.2298/JSC0407571N
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High-performance liquid chromatographic separation of surface flavonoid aglycones in Artemistia annua L. and Artemisia vulgaris L
Nikolova Milena (Department of Applied Botany, Institute of Botany, Bulgarian Academy of Sciences, bl. 23, 'Acad. G. Bonchev', Sofia, Bulgaria)
Gevrenova Renata (Department of Pharmacognosy, Faculty of Pharmacy,Medical University - Sofia, Sofia, Bulgaria)
Ivancheva Stephanka (Department of Applied Botany, Institute of Botany, Bulgarian Academy of Sciences, bl. 23, 'Acad. G. Bonchev', Sofia, Bulgaria)
External flavonoid aglycones from Artemisia annua L. and Artemisia vulgaris L. were analyzed by the HPLC method. The mobile phase was composed of t-butanol-methanol- 20 mmol l-1 phosphate buffer, pH 3.22. The linear gradient elution method within 22 min was applied. The main aglycones in A. annua and A. vulgaris are methyl ethers of quercetagetin and quercetin. Quercetagetin 3,6,7-trimethyl ether in A. annua and quercetin 3,7,3’-trimethyl ether in A. vulgaris are the most abundant compounds.
Keywords: Artemisia annua, Artemisia vulgaris, HPLC, flavonoid aglycones, quercetagetin 3,6,7-trimethyl ether, quercetin 3,7,3’-trimethyl ether