doiSerbia

Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran

Yousefzadi Morteza (Department of Ecology and Systematic, Research Institute of Applied Science, ACECR, Shahid Beheshti University, Tehran, Iran)
Mirjalili Hossein Mohammad (Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Tehran, Iran)
Alnajar Naba (Department of Biology, Faculty of Science, Shahid Beheshti University, Tehran, Iran)
Zeinali Amineh (Department of Ecology and Systematic, Research Institute of Applied Science, ACECR, Shahid Beheshti University, Tehran, Iran)
Parsa Mitra (Department of Ecology and Systematic, Research Institute of Applied Science, ACECR, Shahid Beheshti University, Tehran, Iran)

The genus Dorema (Apiaceae) is represented in the flora of Iran with seven species of which two, D. ammoniacum D. Don. and D. aucheri Boiss. are endemic. Ripe fruits of D. ammoniacum collected just in the deciduous time were subjected to hydrodistillation to yield the essential oil, which was subsequently analyzed by GC and GC-MS. Twenty-nine compounds were identified and quantified, representing 95.1 % of the total oil. (Z)-Ocimenone (22.3 %) and (E)-ocimenone (18.1 %) were the main components of the oil. In vitro antimicrobial activity of the oil was evaluated against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial assay of the oil by the disc diffusion method and the MIC values indicated that the oil exhibited moderate to high antimicrobial activity, especially against B. subtilis and S. epidermidis with MIC value of 3.75 mg ml-1.

Keywords: Dorema ammoniacum, Apiaceae, essential oilcomposition, antimicrobial activity, ocimenone. (Z)-ocimenone, (E)-ocimenone