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Cobiss

Journal of the Serbian Chemical Society 2021 Volume 86, Issue 12, Pages: 1195-1203
https://doi.org/10.2298/JSC210809089I
Full text ( 2925 KB)
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GC-MS-based metabolomics for the detection of adulteration in oregano samples

Ivanović Stefan ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia)
Mandrone Manuela (University of Bologna, Department of Pharmacy and Biotechnology, Bologna, Italy)
Simić Katarina ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia)
Ristić Mirjana (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia)
Todosijević Marina ORCID iD icon (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia)
Mandić Boris ORCID iD icon (University of Belgrade, Faculty of Chemistry, Belgrade, Serbia)
Gođevac Dejan ORCID iD icon (University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia), [email protected]

Oregano is one of the most used culinary herb and it is often adulterated with cheaper plants. In this study, GC–MS was used for identification and quantification of metabolites from 104 samples of oregano (Origanum vulgare and O. onites) adulterated with olive (Olea europaea), venetian sumac (Cotinus coggygria) and myrtle (Myrtus communis) leaves, at five different concentration levels. The metabolomics profiles obtained after the two-step derivatization, involving methoxyamination and silanization, were subjected to multivariate data analysis to reveal markers of adulteration and to build the regression models on the basis of the oregano-to-adulterants mixing ratio. Orthogonal partial least squares enabled detection of oregano adulterations with olive, Venetian sumac and myrtle leaves. Sorbitol levels distinguished oregano samples adulterated with olive leaves, while shikimic and quinic acids were recognized as discrimination factor for adulteration of oregano with venetian sumac. Fructose and quinic acid levels correlated with oregano adulteration with myrtle. Orthogonal partial least squares discriminant analysis enabled discrimination of O. vulgare and O. onites samples, where catechollactate was found to be discriminating metabolite.

Keywords: chromatography, PCA, OPLS, Origanum vulgare, Origanum onites, Olea europaea, Cotinus coggygria, Myrtus communis

Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-9/2021-14/200026