Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Influence of Neutralization Conditions
2.2. Influence of Gel Aging Conditions
2.3. Influence of Cell Density
2.4. Discussion
3. Conclusions
4. Materials and Methods
4.1. Materials and Cells
4.2. Plastically Compressed Collagen Hydrogel Preparation
4.3. Cell Culture Conditions
4.4. Structural and Chemical Characterizations
4.4.1. Scanning Electron Microscopy (SEM)/Energy-Dispersive X-ray (EDX) Spectroscopy
4.4.2. Rheological Studies
4.4.3. Fourier Transform Infra-Red Spectroscopy (FTIR)
4.5. Biological Studies
4.5.1. Cell Viability
4.5.2. Metabolic Activity
4.5.3. Histological Studies
4.6. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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VDMEM (mL) | [DMEM] | pH | [NaOH] (M) | VNaOH (μL) | Final [DMEM] | Gel before Compression | Gel after Compression |
---|---|---|---|---|---|---|---|
0.5 | 1X | 4.5–5 | 0.1 | 350 | 0.5X | spread and leak | crush |
0.5 | 2.5X | 4 | 0.1 | 450 | 0.7X | spread and leak | very thin and fragile |
0.5 | 5X | 3.5 | 0.1 | 600 | 0.9X | stable | very thin and stable |
0.5 | 10X | 3 | 0.1 | 700 | 1.4X | stable | very thin and stable |
1.0 | 1X | 6–7 | 0.1 | 0 | 0.6X | spread and leak | very thin and fragile |
1.0 | 2.5X | 3.5 | 0.1 | 600 | 0.8X | stable | very thin and stable |
1.0 | 5X | 3 | 0.1 | 700 | 1.3X | stable | thin and stable (H) |
1.0 | 10X | <3 | 0.1 | >1000 | - | - | - |
1.5 | 1X | 6–7 | 0.1 | 0 | 0.6X | spread and leak | crush |
1.5 | 2.5X | 3.5 | 0.1 | 700 | 0.9X | spread and leak | very thin and fragile |
1.5 | 5X | 3 | 0.1 | 800 | 1.6X | spread and leak | crush |
VDMEM (mL) | [DMEM] | pH | [NaOH] (M) | VNaOH (μL) | Final [DMEM] | Gel before Compression | Gel after Compression |
---|---|---|---|---|---|---|---|
1.0 | 1X | 3.5–4 | 5 | 200 | 0.3X | spread and leak | very thin and stable |
0.1 | 1000 | ||||||
1.0 | 2.5X | 3 | 5 | 205 | 0.7X | stable | thin and stable |
0.1 | 640 | ||||||
1.0 | 5X | 2–3 | 5 | 210 | 1.2X | spread and leak | very thin and fragile |
0.1 | 700 | ||||||
1.0 | 10X | 2 | 5 | 215 | 2.3X | no gel formed | - |
0.1 | 450 | ||||||
1.0 | 1X | 3.5–4 | 5 | 200 | 0.5X | spread and leak | very thin and stable |
1 | 30 | ||||||
1.0 | 2.5X | 3 | 5 | 205 | 0.8X | stable | thin and stable (I) |
1 | 40 | ||||||
1.0 | 5X | 2–3 | 5 | 210 | 1.2X | spread and leak | very thin and stable |
1 | 60 |
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Mbitta Akoa, D.; Sicard, L.; Hélary, C.; Torrens, C.; Baroukh, B.; Poliard, A.; Coradin, T. Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels. Gels 2024, 10, 130. https://doi.org/10.3390/gels10020130
Mbitta Akoa D, Sicard L, Hélary C, Torrens C, Baroukh B, Poliard A, Coradin T. Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels. Gels. 2024; 10(2):130. https://doi.org/10.3390/gels10020130
Chicago/Turabian StyleMbitta Akoa, Daline, Ludovic Sicard, Christophe Hélary, Coralie Torrens, Brigitte Baroukh, Anne Poliard, and Thibaud Coradin. 2024. "Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels" Gels 10, no. 2: 130. https://doi.org/10.3390/gels10020130