Journal of the Serbian Chemical Society 2011 Volume 76, Issue 9, Pages: 1229-1236
https://doi.org/10.2298/JSC110120106M
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Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells
Milisavljević Mira Đ. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Timotijević Gordana S. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Nikolić Dragana B. 
(Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Samardžić Jelena T. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Maksimović Vesna R. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
The recombinant aspartic proteinase-like protein (FeAPL1-His6) was
overexpressed in the tobacco BY-2 cell line and the expected pepstatin
A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry
and protein gel blot analysis of the transformed BY-2 cells and their
protoplasts showed extracellular localization of rFeAPL1-His6 in the cell
wall. Based on the obtained results, potential functions of FeAPL1 are
discussed.
Keywords: aspartic proteinase, buckwheat, BY-2 cells, cell wall