Jelena Janjic
Duquesne University, Mylan School of Pharmacy, Faculty Member
Research Interests:
Research Interests: Organic Chemistry, Cell Cycle, Pharmaceutical Chemistry, Estrogen Receptor, Cell line, and 14 moreIn Vitro, Humans, Amides, Cell Cycle regulation, Protein isoforms, Cell Proliferation, Structure activity Relationship, Protein Binding, Chemical Synthesis, Antineoplastic Agents, Ligands, Amine, Breast Cancer Cells, and Estrogen receptor-beta
We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes... more
We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 nm) were readily taken up by both phagocytic and non-phagocytic cells in vitro after a short (approximately 3 h) co-incubation. Following cell administration in vivo, 19F MRI selectively visualizes cell migration. Exemplary in vivo MRI images are presented of T cells labeled with a dual-mode nanoemulsion in a BALB/c mouse. Fluorescence detection enables fluorescent microscopy and FACS analysis of labeled cells, as demonstrated in several immune cell types including Jurkat cells, primary T cells and dendritic cells. The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro.
Research Interests: Magnetic Resonance Imaging, Fluorescence, Fluorescence Microscopy, Cell Migration, Fluorescent Dyes and Reagents, and 16 moreMagnetic Resonance Spectroscopy, Cell line, Mice, Animals, Nanostructures, Dendritic cell, CHEMICAL SCIENCES, T lymphocytes, Fluorine, Emulsions, Cell Proliferation, Sensitivity and Specificity, Cell Survival, Ethers, Contrast Media, and Molecular Structure
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A new structural scaffold for antiestrogens was identified from the cell-based screening of transcriptional regulation properties of a 67-member library of homoallylic amides, allylic amides, and C-cyclopropylalkylamides.... more
A new structural scaffold for antiestrogens was identified from the cell-based screening of transcriptional regulation properties of a 67-member library of homoallylic amides, allylic amides, and C-cyclopropylalkylamides. C-Cyclopropylalkylamide 3a (O-ethyl-N-{2-[(1S*,2R*)-2-{(R*)-[(diphenylphosphinoyl)amino](phenyl)methyl}cyclopropyl]ethyl}-N-[(4-methylphenyl)sulfonyl]carbamate) had antagonistic activity similar to that of tamoxifen and was further evaluated. Compound 3a inhibited estradiol-induced proliferation of the ER-positive MCF-7 cells but had no effect on ER-negative MDA-MB231 human breast cancer cells. Furthermore, high micromolar concentrations of 3a exhibited minimal cytotoxicity to the ER-negative line. The biological activities of the enantiomers of 3a did not differ from one another nor from that of racemic 3a.
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Research Interests:
Research Interests: Clinical Trial, Cancer, Magnetic Resonance Imaging, Flow Cytometry, Nanoparticle, and 22 moreDendritic Cells, Cell Differentiation, Humans, Infectious Disease, Mice, Animals, Functional Assessment, Dendritic cell, Cell Viability, Phenotype, Clinical Sciences, Chemokine, Fluorine, Monocytes, Ether, Emulsions, Cell Survival, Functional Testing, Interleukin, Lymph nodes, Magnetic resonance image, and Cell Membrane
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In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo19F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled... more
In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo19F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled cells are introduced into a subject and tracked using 19F MRI or NMR spectroscopy. In the same imaging session, a high-resolution, conventional (1H) image can be used to place the 19F-labeled cells into anatomical context. Perfluorocarbon-based 19F cell tracking is a useful technology because of the high specificity for labeled cells, ability to quantify cell accumulations, and biocompatibility. This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy. Copyright © 2009 John Wiley & Sons, Inc.For further resources related to this article, please visit the WIREs website.
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For Abstract see ChemInform Abstract in Full Text.
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Microwave-assisted synthesis of allylic amines: considerable rate acceleration in the hydrozirconation?transmetalation?aldimine addition sequenceElectronic supplementary information (ESI) available: experimental procedures and 1H and 13C NMR spectra for all new compounds. See http://www.rsc.org/s...more