Maps of plasmids for SARS-CoV-2 lentiviral pseudotyping. The links go to Genbank plasmid maps that can be opened in programs such as ApE or Benchling.
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HDM_IDTSpike_fixK: plasmid expressing the Spike protein from SARS-CoV-2 with the sequence codon optimized (#2710 in Bloom lab plasmid log).
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HDM_IDTSpike_fixK_HA_Tail: plasmid expressing the Spike protein from SARS-CoV-2 with the seuqence codon optimized and the cytoplasmic tail replaced by that from influenza HA (#2707 in Bloom lab plasmid log).
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HDM-nCoV-Spike-IDTopt-ALAYT: plasmid expressing the Spike protein from SARS-CoV-2 with the sequence codon-optimized by IDT and two mutations in the last five amino-acids (so that the C-terminal sequence is ALAYT). This is plasmid #2703 in Bloom lab plasmid log.
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pHAGE2_CMV_ZsGreen_W: lentiviral backbone expressing ZsGreen under a CMV promoter (#25 in Bloom lab plasmid log).
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pHAGE-CMV-Luc2-IRES-ZsGreen-W: lentiviral backbone expressing Luciferase followed by an IRES-ZsGreen under a CMV promoter (#2728 in Bloom lab plasmid log).
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pHAGE2_EF1aInt_ACE2_WT: lentiviral backbone expressing human ACE2 under a EF1a promoter containing an intron (#2653 in Bloom lab plasmid log).
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HDM_Hgpm2: lentiviral helper plasmid expressing HIV Gag-Pol under a CMV promoter (#26 in Bloom lab plasmid log).
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HDM_tat1b: lentiviral plasmid expressing HIV Tat under a CMV promoter (#27 in Bloom lab plasmid log).
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pRC_CMV_Rev1b: lentiviral plasmid expressing HIV Rev under a CMV promoter (#28 in Bloom lab plasmid log).
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HDM_SARS2_Spikedelta18: plasmid expressing the Spike protein from SARS-CoV-2 with the sequence codon optimized and the last 18 amino acids deleted (#2776 in Bloom lab plasmid log).
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HDM_SARS2_Spikedelta21: plasmid expressing the Spike protein from SARS-CoV-2 with the sequence codon optimized and the last 21 amino acids deleted (#2777 in Bloom lab plasmid log).
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HDM_Spikedelta21_D614G: plasmid expressing the Spike protein from SARS-CoV-2 with the sequence codon optimized, the last 21 amino acids deleted, and the D614G mutation (#2800 in Bloom lab plasmid log).