The aim of the current research was to investigate the deuterium depleted water (DDW) effect on a... more The aim of the current research was to investigate the deuterium depleted water (DDW) effect on animals treated with anticancer drugs Vinblastine, Cyclophosphamide, 5-Fluorouracil and Farmarubicine. There were used two groups of 10 rats for each cytostatic, one group received 60 ppm DDW as daily diet before, during and after chemotherapy and the second group received tap water in the same conditions. Other two groups housed in the same conditions received DDW and tap water without any medication. There were recorded differences in the amount of serum glycoproteins and also the glycosylation degree and pattern. DDW administration induced a significant decrease of serum total glycoproteins and of glycosylation degree in all animals, both cytostatics treated or not. Fucose Raphanus sativus lectin interaction revealed that DDW induced also changes in the glycosylated domain, because it interacted only with the sera from 5-Fluorouracil treated rats that had not received DDW.
Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by various bacteria ... more Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by various bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we describe the isolation and characterization of Pseudomans putida P5 strain, as well as of some of its derivatives (mutants and recombinants) regarding the accumulation of PHAs. All the strains were subjected to fermentation and showed PHA accumulation with glucose, gluconate or octanoate as carbon source. In order to obtain PHA produced by these strains, a special procedure was developed, which allowed 80% PHA recovered. Differences in msc-PHA accumulation were observed in chemically induced mutants. RAPD analysis of these mutants proved that some rearrangements are produced as result of mutagenesis. The best results concerning msc-PHA accumulation were obtained when 0.4% octanoate was used. Different other lipid substrates (vegetal oils) were tested in order to es...
There were performed in vitro interactions of five plant lectins purified by affinity chromatogra... more There were performed in vitro interactions of five plant lectins purified by affinity chromatography with seven pathogenic bacteria cultivated in liquid media. The positive results were scored by the degree of bacterial cells agglutination. There have been noticed strong agglutinations of Salmonella typhymurium and Salmonella gallinarum with the lectins isolated from pumpkin seeds, potato buds and potato tubers. Escherichia coli and Pseudomonas aeruginosa were better agglutinated by vegetable marrow and pumpkin seeds lectins and weaker by potato buds and tubers lectins. Glutaraldehyde immobilization of the lectin isolated from potato buds resulted in an insoluble polymer suspension. The interaction of the lectin polymer suspension with the liquid cultures of Salmonella and Pseudomonas was quantified by measuring the OD (660 nm) at time 0 and after 10 minutes of standing. The decrease of the OD demonstrated that immobilized insoluble lectin polymer bound bacterial cells and the aggre...
The aim of the current research was to investigate the deuterium depleted water (DDW) effect on a... more The aim of the current research was to investigate the deuterium depleted water (DDW) effect on animals treated with anticancer drugs Vinblastine, Cyclophosphamide, 5-Fluorouracil and Farmarubicine. There were used two groups of 10 rats for each cytostatic, one group received 60 ppm DDW as daily diet before, during and after chemotherapy and the second group received tap water in the same conditions. Other two groups housed in the same conditions received DDW and tap water without any medication. There were recorded differences in the amount of serum glycoproteins and also the glycosylation degree and pattern. DDW administration induced a significant decrease of serum total glycoproteins and of glycosylation degree in all animals, both cytostatics treated or not. Fucose Raphanus sativus lectin interaction revealed that DDW induced also changes in the glycosylated domain, because it interacted only with the sera from 5-Fluorouracil treated rats that had not received DDW.
This Review Article is focused on the action of the reactive oxygenated species in inducing oxida... more This Review Article is focused on the action of the reactive oxygenated species in inducing oxidative injury of the lipid membrane components, as well as on the ability of antioxidants (of different structures and sources, and following different mechanisms of action) in fighting against oxidative stress. Oxidative stress is defined as an excessive production of reactive oxygenated species that cannot be counteracted by the action of antioxidants, but also as a perturbation of cell redox balance. Reactive oxygenated/nitrogenated species are represented by superoxide anion radical, hydroxyl, alkoxyl and lipid peroxyl radicals, nitric oxide and peroxynitrite. Oxidative stress determines structure modifications and function modulation in nucleic acids, lipids and proteins. Oxidative degradation of lipids yields malondialdehyde and 4-hydroxynonenal, but also isoprostanes, from unsaturated fatty acids. Protein damage may occur with thiol oxidation, carbonylation, side-chain oxidation, fragmentation, unfolding and misfolding, resulting activity loss. 8-hydroxydeoxyguanosine is an index of DNA damage. The involvement of the reactive oxygenated/nitrogenated species in disease occurrence is described. The unbalance between the oxidant species and the antioxidant defense system may trigger specific factors responsible for oxidative damage in the cell: over-expression of oncogene genes, generation of mutagen compounds, promotion of atherogenic activity, senile plaque occurrence or inflammation. This leads to cancer, neurodegeneration, cardiovascular diseases, diabetes, kidney diseases. The concept of antioxidant is defined, along with a discussion of the existent classification criteria: enzymatic and non-enzymatic, preventative or repair-systems, endogenous and exogenous, primary and secondary, hydrosoluble and liposoluble, natural or synthetic. Primary antioxidants are mainly chain breakers, able to scavenge radical species by hydrogen donation. Secondary antioxidants are singlet oxygen quenchers, peroxide decomposers, metal chelators, oxidative enzyme inhibitors or UV radiation absorbers. The specific mechanism of action of the most important representatives of each antioxidant class (endogenous and exogenous) in preventing or inhibiting particular factors leading to oxidative injury in the cell, is then reviewed. Mutual influences, including synergistic effects are presented and discussed. Prooxidative influences likely to occur, as for instance in the presence of transition metal ions, are also reminded.
The researches of our group focused on the investigation of the effects of some lectins introduce... more The researches of our group focused on the investigation of the effects of some lectins introduced in poultry nutrition in order to manipulate their immune response. The used lectins were isolated by the biochemists in the faculty's laboratory, by affinity chromatography, from Wheat Germs, Solanum tuberosum buds, and Cucurbita pepo ovifera. The lectins were introduced in the drinking water of
The aim of the current research was to investigate the deuterium depleted water (DDW) effect on a... more The aim of the current research was to investigate the deuterium depleted water (DDW) effect on animals treated with anticancer drugs Vinblastine, Cyclophosphamide, 5-Fluorouracil and Farmarubicine. There were used two groups of 10 rats for each cytostatic, one group received 60 ppm DDW as daily diet before, during and after chemotherapy and the second group received tap water in the same conditions. Other two groups housed in the same conditions received DDW and tap water without any medication. There were recorded differences in the amount of serum glycoproteins and also the glycosylation degree and pattern. DDW administration induced a significant decrease of serum total glycoproteins and of glycosylation degree in all animals, both cytostatics treated or not. Fucose Raphanus sativus lectin interaction revealed that DDW induced also changes in the glycosylated domain, because it interacted only with the sera from 5-Fluorouracil treated rats that had not received DDW.
Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by various bacteria ... more Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by various bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we describe the isolation and characterization of Pseudomans putida P5 strain, as well as of some of its derivatives (mutants and recombinants) regarding the accumulation of PHAs. All the strains were subjected to fermentation and showed PHA accumulation with glucose, gluconate or octanoate as carbon source. In order to obtain PHA produced by these strains, a special procedure was developed, which allowed 80% PHA recovered. Differences in msc-PHA accumulation were observed in chemically induced mutants. RAPD analysis of these mutants proved that some rearrangements are produced as result of mutagenesis. The best results concerning msc-PHA accumulation were obtained when 0.4% octanoate was used. Different other lipid substrates (vegetal oils) were tested in order to es...
There were performed in vitro interactions of five plant lectins purified by affinity chromatogra... more There were performed in vitro interactions of five plant lectins purified by affinity chromatography with seven pathogenic bacteria cultivated in liquid media. The positive results were scored by the degree of bacterial cells agglutination. There have been noticed strong agglutinations of Salmonella typhymurium and Salmonella gallinarum with the lectins isolated from pumpkin seeds, potato buds and potato tubers. Escherichia coli and Pseudomonas aeruginosa were better agglutinated by vegetable marrow and pumpkin seeds lectins and weaker by potato buds and tubers lectins. Glutaraldehyde immobilization of the lectin isolated from potato buds resulted in an insoluble polymer suspension. The interaction of the lectin polymer suspension with the liquid cultures of Salmonella and Pseudomonas was quantified by measuring the OD (660 nm) at time 0 and after 10 minutes of standing. The decrease of the OD demonstrated that immobilized insoluble lectin polymer bound bacterial cells and the aggre...
The aim of the current research was to investigate the deuterium depleted water (DDW) effect on a... more The aim of the current research was to investigate the deuterium depleted water (DDW) effect on animals treated with anticancer drugs Vinblastine, Cyclophosphamide, 5-Fluorouracil and Farmarubicine. There were used two groups of 10 rats for each cytostatic, one group received 60 ppm DDW as daily diet before, during and after chemotherapy and the second group received tap water in the same conditions. Other two groups housed in the same conditions received DDW and tap water without any medication. There were recorded differences in the amount of serum glycoproteins and also the glycosylation degree and pattern. DDW administration induced a significant decrease of serum total glycoproteins and of glycosylation degree in all animals, both cytostatics treated or not. Fucose Raphanus sativus lectin interaction revealed that DDW induced also changes in the glycosylated domain, because it interacted only with the sera from 5-Fluorouracil treated rats that had not received DDW.
This Review Article is focused on the action of the reactive oxygenated species in inducing oxida... more This Review Article is focused on the action of the reactive oxygenated species in inducing oxidative injury of the lipid membrane components, as well as on the ability of antioxidants (of different structures and sources, and following different mechanisms of action) in fighting against oxidative stress. Oxidative stress is defined as an excessive production of reactive oxygenated species that cannot be counteracted by the action of antioxidants, but also as a perturbation of cell redox balance. Reactive oxygenated/nitrogenated species are represented by superoxide anion radical, hydroxyl, alkoxyl and lipid peroxyl radicals, nitric oxide and peroxynitrite. Oxidative stress determines structure modifications and function modulation in nucleic acids, lipids and proteins. Oxidative degradation of lipids yields malondialdehyde and 4-hydroxynonenal, but also isoprostanes, from unsaturated fatty acids. Protein damage may occur with thiol oxidation, carbonylation, side-chain oxidation, fragmentation, unfolding and misfolding, resulting activity loss. 8-hydroxydeoxyguanosine is an index of DNA damage. The involvement of the reactive oxygenated/nitrogenated species in disease occurrence is described. The unbalance between the oxidant species and the antioxidant defense system may trigger specific factors responsible for oxidative damage in the cell: over-expression of oncogene genes, generation of mutagen compounds, promotion of atherogenic activity, senile plaque occurrence or inflammation. This leads to cancer, neurodegeneration, cardiovascular diseases, diabetes, kidney diseases. The concept of antioxidant is defined, along with a discussion of the existent classification criteria: enzymatic and non-enzymatic, preventative or repair-systems, endogenous and exogenous, primary and secondary, hydrosoluble and liposoluble, natural or synthetic. Primary antioxidants are mainly chain breakers, able to scavenge radical species by hydrogen donation. Secondary antioxidants are singlet oxygen quenchers, peroxide decomposers, metal chelators, oxidative enzyme inhibitors or UV radiation absorbers. The specific mechanism of action of the most important representatives of each antioxidant class (endogenous and exogenous) in preventing or inhibiting particular factors leading to oxidative injury in the cell, is then reviewed. Mutual influences, including synergistic effects are presented and discussed. Prooxidative influences likely to occur, as for instance in the presence of transition metal ions, are also reminded.
The researches of our group focused on the investigation of the effects of some lectins introduce... more The researches of our group focused on the investigation of the effects of some lectins introduced in poultry nutrition in order to manipulate their immune response. The used lectins were isolated by the biochemists in the faculty's laboratory, by affinity chromatography, from Wheat Germs, Solanum tuberosum buds, and Cucurbita pepo ovifera. The lectins were introduced in the drinking water of
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