Fabrication of complex structures usually requires a combination of different advanced manufactur... more Fabrication of complex structures usually requires a combination of different advanced manufacturing techniques at different length scales. In this work, we discuss the structure-properties relationship of recently developed biodegradable poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers modified with hydrophilic poly(ethylene glycol) (PEG) towards their processibility via 3D printing and electrospinning. Notably, filaments suitable for 3D printing with 1.75mm in diameter were fabricated directly from the reactor after the synthesis of copolymers through extruding polymer melt into a water bath thus overcoming the post-processing. Two series of copolymers containing 60 wt% and 70 wt% of hard PBS content were synthesized using magnesium-titanium butoxide as heterometallic catalyst. Differential scanning calorimetry indicated biphasic morphology with low glass transition temperature and high melting point, which were dependent from the PBS hard segments content. Cryst...
Advanced manufacturing techniques for fabrication of tubular structures using biodegradable and b... more Advanced manufacturing techniques for fabrication of tubular structures using biodegradable and biocompatible materials are crucial for the progress of tissue engineering. However, using 3D printing or electrospinning alone to fabricate multilayered tubular scaffolds that mimic the structural features of the tubular tissue (for example coronary artery) is not always sufficient and the selection of suitable biomaterials is still an important criterion. In order to address these challenges, first, we selected new segmented poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers of different segmental composition (70 wt% and 60 wt% of hard segments, respectively) which were modified with poly(ethylene glycol) (PEG). The effect of the segmental composition of copolymers on their enzymatic degradation, bioactivity, and cytotoxicity was investigated. All polymers showed a decrease in molecular weight (up to 40%) as determined by GPC, and mass loss up to 10% after 20 days of enz...
In this study, the synthesis and physico-chemical properties of bio-based poly(butylene succinate... more In this study, the synthesis and physico-chemical properties of bio-based poly(butylene succinate-dilinoleic succinate)(PBS-DLS) modified with poly(ethylene glycol)(PEG) differing in segmental composition of respective units (PBS-DLS-PEG) are discussed in order to determine their structure-properties relationship and translate it to their electrospinability and 3-D printability. The PBS-DLS copolymers, containing bio-based succinic acid and dilinoleic diol, were successfully synthesized by two-step polycondensation, and collected directly from the reactor as crystallized filaments suitable for 3D printing. Chemical characterization indicated the formation of expected functional ester groups. Crystallized copolymers revealed spherulitic morphology and phase transitions typical for thermoplastic elastomers. Interestingly, incorporation of 5 wt% of PEG into copolymers containing higher hard segments content (70 wt%) disturbed the banded spherulitic morphology of copolymers, contributed...
Introduction: Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is ... more Introduction: Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint disease characterized by reduction of joint cartilage thickness, demolition of cartilage surface and new bone formation. To overcome these problems, the purpose of the current research was to evaluate and compare the in vivo effects of Synovial membrane-derived MSCs (SMMSCs), platelet rich plasma (PRP) and conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy. Methods: For the first step, SMMSCs were isolated and characterized. Also, secretome was collected from SMMSCs culture. Furthermore, PRP was collect from the rat heart venous blood. Second, two injection of collagenase II with an interval of 3 days was performed in the knee intra- articular space to induce osteoarthritis. Two weeks later, animals were randomly divided into 6 groups. Control group without treatment, positive group: taken an intra-articular Hyalgan injecti...
Purpose Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the mo... more Purpose Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint disease characterized by reduction of joint cartilage thickness, demolition of cartilage surface and new bone formation. To overcome these problems, the purpose of the current research was to evaluate and compare the in vivo effects of synovial membrane-derived mesenchymal stem cell (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy. Methods For the first step, SMMSCs were isolated and characterized. Also, secretome was collected from SMMSCs culture. Furthermore, PRP was collect from the rat heart venous blood. Second, two injection of collagenase II with an interval of 3Â days was performed in the knee intra-articular space to induce osteoarthritis. Two weeks later, animals were randomly divided into 6 groups. Control group without treatment, positive group: taken an intra-articular sodium...
Category: Ankle; Ankle Arthritis; Basic Sciences/Biologics; Trauma Introduction/Purpose: Osteoart... more Category: Ankle; Ankle Arthritis; Basic Sciences/Biologics; Trauma Introduction/Purpose: Osteoarthritis (OA) is the most common degenerative joint disorder, characterized mainly by progressive articular cartilage degradation, subchondral bone thickening, osteophyte formation, and synovial inflammation. About 80% of ankle OA is believed to be post-traumatic and affects the younger population leading to a higher risk of disabilities during life. Mesenchymal stem cells (MSCs) have shown promising effects in preventing the degenerative process of OA via paracrine effect and great differentiation potential. Recently, using chondrocyte conditioned medium (CCM) that contains all necessary secretomes was shown to enhance MSC differentiation and regeneration of the cartilage tissue. In this study, we aimed to assess the effects of intraarticular injection of MSCs plus CCM on the degenerative process of monoiodoacetate (MIA)-induced ankle OA in rats. Methods: Fifty male rats were randomly div...
Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue eng... more Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue engineering, have been proposed for oral ulcerative lesions. We investigated the effects of adipose tissue-derived stem cells (ADSCs) seeded onto the curcumin-loaded collagen scaffold in the mucosal healing of oral ulcers in rats. Materials and Methods: The current experimental study was conducted on 40 male Sprague-Dawley rats. Oral ulcers were created over both sides of buccal mucosa, and the rats were randomly divided into four equal groups: 1) an untreated group (negative control); 2) Teriadent-treated group (positive control); 3) group treated with curcumin-loaded collagen scaffold; and 4) group received the ADSCs (3 × 106 cells) seeded onto the curcumin-loaded collagen scaffold. Rats were sacrificed on 3rd and 7th day after ulceration for histopathological examination as well as measurement of tissue levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and Interleukin-1 beta (...
Statement of the Problem: Tissue engineering was an idea, today it has become a potential therapy... more Statement of the Problem: Tissue engineering was an idea, today it has become a potential therapy for several tissues in dentistry, such as periodontal disease and oral mucosa. Purpose: In this experimental study, periodontal regeneration is one of the earliest clinical disciplines that has achieved therapeutic application in tissue engineering. The aim of the present study was to prepare electrospun Poly (3-hydroxybutyrate) (PHB)/1% Carbon nanotubes (CNTs) scaffolds for periodontal regeneration. Materials and Method: 1% w/v of CNTs was added to the polymer solutions and electrospinned. Physical properties of the scaffolds were evaluated by scanning electron microscopy (SEM) and universal testing machine. Chemical characterization of the scaffolds was also assessed by Fourier-transform infrared spectroscopy (FTIR). Biological properties of the scaffolds were also evaluated in vitro by culturing periodontal ligament stem cells (PDLSCs) on the scaffolds for 10 days and in vivo by Impl...
In this study, the synthesis and physico-chemical properties of poly(butylene succinate-dilinolei... more In this study, the synthesis and physico-chemical properties of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers modified with hydrophilic poly(ethylene glycol)(PEG) differing in segmental composition are discussed in order to determine their structure-properties relationship and translate it to their processability. The copolymers, containing bio-based monomers such as succinic acid and dilinoleic diol, were successfully synthesized by two-step transesterification and polycondensation, and collected directly from the reactor as crystallized filaments suitable for 3D printing. Chemical characterization indicated the formation of expected functional ester groups and incorporation of PEG into polymer chain. Crystallized copolymers revealed spherulitic morphology and phase transitions which were dependent from the segmental composition. Interestingly, incorporation of 5 wt% PEG into copolymers containing higher hard segments content (70 wt%) disturbed the banded spher...
Fabrication of complex structures usually requires a combination of different advanced manufactur... more Fabrication of complex structures usually requires a combination of different advanced manufacturing techniques at different length scales. In this work, we discuss the structure-properties relationship of recently developed biodegradable poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers modified with hydrophilic poly(ethylene glycol) (PEG) towards their processibility via 3D printing and electrospinning. Notably, filaments suitable for 3D printing with 1.75mm in diameter were fabricated directly from the reactor after the synthesis of copolymers through extruding polymer melt into a water bath thus overcoming the post-processing. Two series of copolymers containing 60 wt% and 70 wt% of hard PBS content were synthesized using magnesium-titanium butoxide as heterometallic catalyst. Differential scanning calorimetry indicated biphasic morphology with low glass transition temperature and high melting point, which were dependent from the PBS hard segments content. Cryst...
Advanced manufacturing techniques for fabrication of tubular structures using biodegradable and b... more Advanced manufacturing techniques for fabrication of tubular structures using biodegradable and biocompatible materials are crucial for the progress of tissue engineering. However, using 3D printing or electrospinning alone to fabricate multilayered tubular scaffolds that mimic the structural features of the tubular tissue (for example coronary artery) is not always sufficient and the selection of suitable biomaterials is still an important criterion. In order to address these challenges, first, we selected new segmented poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers of different segmental composition (70 wt% and 60 wt% of hard segments, respectively) which were modified with poly(ethylene glycol) (PEG). The effect of the segmental composition of copolymers on their enzymatic degradation, bioactivity, and cytotoxicity was investigated. All polymers showed a decrease in molecular weight (up to 40%) as determined by GPC, and mass loss up to 10% after 20 days of enz...
In this study, the synthesis and physico-chemical properties of bio-based poly(butylene succinate... more In this study, the synthesis and physico-chemical properties of bio-based poly(butylene succinate-dilinoleic succinate)(PBS-DLS) modified with poly(ethylene glycol)(PEG) differing in segmental composition of respective units (PBS-DLS-PEG) are discussed in order to determine their structure-properties relationship and translate it to their electrospinability and 3-D printability. The PBS-DLS copolymers, containing bio-based succinic acid and dilinoleic diol, were successfully synthesized by two-step polycondensation, and collected directly from the reactor as crystallized filaments suitable for 3D printing. Chemical characterization indicated the formation of expected functional ester groups. Crystallized copolymers revealed spherulitic morphology and phase transitions typical for thermoplastic elastomers. Interestingly, incorporation of 5 wt% of PEG into copolymers containing higher hard segments content (70 wt%) disturbed the banded spherulitic morphology of copolymers, contributed...
Introduction: Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is ... more Introduction: Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint disease characterized by reduction of joint cartilage thickness, demolition of cartilage surface and new bone formation. To overcome these problems, the purpose of the current research was to evaluate and compare the in vivo effects of Synovial membrane-derived MSCs (SMMSCs), platelet rich plasma (PRP) and conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy. Methods: For the first step, SMMSCs were isolated and characterized. Also, secretome was collected from SMMSCs culture. Furthermore, PRP was collect from the rat heart venous blood. Second, two injection of collagenase II with an interval of 3 days was performed in the knee intra- articular space to induce osteoarthritis. Two weeks later, animals were randomly divided into 6 groups. Control group without treatment, positive group: taken an intra-articular Hyalgan injecti...
Purpose Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the mo... more Purpose Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint disease characterized by reduction of joint cartilage thickness, demolition of cartilage surface and new bone formation. To overcome these problems, the purpose of the current research was to evaluate and compare the in vivo effects of synovial membrane-derived mesenchymal stem cell (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy. Methods For the first step, SMMSCs were isolated and characterized. Also, secretome was collected from SMMSCs culture. Furthermore, PRP was collect from the rat heart venous blood. Second, two injection of collagenase II with an interval of 3Â days was performed in the knee intra-articular space to induce osteoarthritis. Two weeks later, animals were randomly divided into 6 groups. Control group without treatment, positive group: taken an intra-articular sodium...
Category: Ankle; Ankle Arthritis; Basic Sciences/Biologics; Trauma Introduction/Purpose: Osteoart... more Category: Ankle; Ankle Arthritis; Basic Sciences/Biologics; Trauma Introduction/Purpose: Osteoarthritis (OA) is the most common degenerative joint disorder, characterized mainly by progressive articular cartilage degradation, subchondral bone thickening, osteophyte formation, and synovial inflammation. About 80% of ankle OA is believed to be post-traumatic and affects the younger population leading to a higher risk of disabilities during life. Mesenchymal stem cells (MSCs) have shown promising effects in preventing the degenerative process of OA via paracrine effect and great differentiation potential. Recently, using chondrocyte conditioned medium (CCM) that contains all necessary secretomes was shown to enhance MSC differentiation and regeneration of the cartilage tissue. In this study, we aimed to assess the effects of intraarticular injection of MSCs plus CCM on the degenerative process of monoiodoacetate (MIA)-induced ankle OA in rats. Methods: Fifty male rats were randomly div...
Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue eng... more Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue engineering, have been proposed for oral ulcerative lesions. We investigated the effects of adipose tissue-derived stem cells (ADSCs) seeded onto the curcumin-loaded collagen scaffold in the mucosal healing of oral ulcers in rats. Materials and Methods: The current experimental study was conducted on 40 male Sprague-Dawley rats. Oral ulcers were created over both sides of buccal mucosa, and the rats were randomly divided into four equal groups: 1) an untreated group (negative control); 2) Teriadent-treated group (positive control); 3) group treated with curcumin-loaded collagen scaffold; and 4) group received the ADSCs (3 × 106 cells) seeded onto the curcumin-loaded collagen scaffold. Rats were sacrificed on 3rd and 7th day after ulceration for histopathological examination as well as measurement of tissue levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and Interleukin-1 beta (...
Statement of the Problem: Tissue engineering was an idea, today it has become a potential therapy... more Statement of the Problem: Tissue engineering was an idea, today it has become a potential therapy for several tissues in dentistry, such as periodontal disease and oral mucosa. Purpose: In this experimental study, periodontal regeneration is one of the earliest clinical disciplines that has achieved therapeutic application in tissue engineering. The aim of the present study was to prepare electrospun Poly (3-hydroxybutyrate) (PHB)/1% Carbon nanotubes (CNTs) scaffolds for periodontal regeneration. Materials and Method: 1% w/v of CNTs was added to the polymer solutions and electrospinned. Physical properties of the scaffolds were evaluated by scanning electron microscopy (SEM) and universal testing machine. Chemical characterization of the scaffolds was also assessed by Fourier-transform infrared spectroscopy (FTIR). Biological properties of the scaffolds were also evaluated in vitro by culturing periodontal ligament stem cells (PDLSCs) on the scaffolds for 10 days and in vivo by Impl...
In this study, the synthesis and physico-chemical properties of poly(butylene succinate-dilinolei... more In this study, the synthesis and physico-chemical properties of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers modified with hydrophilic poly(ethylene glycol)(PEG) differing in segmental composition are discussed in order to determine their structure-properties relationship and translate it to their processability. The copolymers, containing bio-based monomers such as succinic acid and dilinoleic diol, were successfully synthesized by two-step transesterification and polycondensation, and collected directly from the reactor as crystallized filaments suitable for 3D printing. Chemical characterization indicated the formation of expected functional ester groups and incorporation of PEG into polymer chain. Crystallized copolymers revealed spherulitic morphology and phase transitions which were dependent from the segmental composition. Interestingly, incorporation of 5 wt% PEG into copolymers containing higher hard segments content (70 wt%) disturbed the banded spher...
Uploads