Submit TALEN Targeter

Sequence

Enter up to 2 megabases total in any number of sequences. Sequences should be in FASTA format and include a header line that starts with a '>'. For larger data sets, attach a file instead of pasting text in.

Sequence File

Files must be less than 2 MB.
Allowed file types: fasta.

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Use a Preset Architecture

Preset Architecture

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Provide Custom Spacer/RVD Lengths

Spacer

Minimum Spacer Length

Maximum Spacer Length

Repeat Array

Minimum Repeat Array Length

Maximum Repeat Array Length

G Substitute *

New data show that NH binds G more specifically than NN (which the tool used previously). See Cong et al. (2012) and Streubel et al. (2012).

Filter Options *

Reyon, et al. (2012) recently provided evidence that the previously available design guidelines have little effect on TALEN efficiency. Therefore, they have been removed from this version. They are still available with the old version.

Cut Site Position

Streubel et al. guidelines *

Require at least 1/4 of bases in each TALE to be C+G and disallow runs of 6 or more A+T in each TALE, as suggested by Streubel et al. (2012).

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Count Predicted Targets in a Genome / Promoterome

Pre-loaded Sequence

Genome / promoterome to count predicted target sites for.

Count Predicted Targets in an NCBI Sequence

NCBI ID

NCBI ID to count predicted target sites for. See this page for help finding a usable ID.

Scoring Matrix *

Upstream Base *

Select the base that should precede target sites on the 5' end.

You can, but are not required to, enter an email address here to notify once your results are ready.

Expires *

How long your results will be available for downloading from the server. After this time, your results will be permanently deleted.

TAL Effector Nucleotide Targeter 2.0

Submit TALEN Targeter

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