Pharmacy

Chitosan acetate films have been prepared using chitosans from shrimps (Pleuroncodes monodon) of low and high molecular weight (LMv = 68,000 g/mol and HMv = 232,000 g/mol) and deacetylation degree of 80 and 100%, respectively. The chitosan films were obtained by addition of several additives to acetic acid chitosan solutions, such as: glycerol, oleic acid and linoleic acid in different proportions. The pH of the solutions before casting ranged from 5.0 to 6.0. The composite film thickness are reported. The films have been analyzed by FTIR showing characteristic bands corresponding to the additives. The scanning electron microscopy (SEM) studies reveals the different morphology of the composite films. The films exhibit different physical properties depending upon the additives and/or mixture of them. The addition of glycerol to composite improves the elasticity of the films. The swelling in glucose and saline solutions for several films was evaluated, being higher in the glucose solution. The bactericide test against Staphylococcus aureus, Pseudomona aeruginosa and Acinetobacter baumanii in plates with either blood and or agar tripticase showed that the molecular weight influences on the bactericidal properties of the chitosan composite films and over its effect against gram positive and gram negative bacteria. Medical applications of the composite films were done in patients with burns, ulcers and injuries, the films containing glycerol showed good adhesion in comparison with those without it. The composite films tested were mainly three (1) chitosan acetate with glycerol, (2) chitosan acetate with oleic acid and (3) chitosan acetate with glycerol and oleic acid. Excellent results in the skin recovery were obtained after 7–10 days. Since the chitosan is biodegradable by the body enzymes it does not need to be removed and increases the gradual grows of the damage tissues.

A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal ...

 Nitric oxide (NO) signaling appears to play a vital role in wound healing associated to improve collagen and angiogenesis. A burn wound model was used to evaluate the effects of a chitosan films on histopathological features, nitric oxide synthase (NOS) activity, and quantification of neoformed capillaries assessed with CD34. Bilateral burns (n = 16) were made on adult Sprague-Dawley rats. The lesions on the right side of the rats were treated with chitosan films, and the lesions on the left side of the same rats were treated with gauze with NaCl 0.9% as a control. Histological analysis revealed accelerated burn wound healing supported by significant differences in acute inflammation, collagen, and granulation tissue formation in chitosan-treated burns. Additionally, chitosan-treated burns were associated with higher CD34 immunoreactivity antibody supported by significant differences. This analysis of NOS activity was statistically significant on treated burns in the seco...

Este artículo es el resultado de un proyecto colaborativo entre la Universidad de Guadalajara, un ingeniero de la compañía Intel, una asociación de médicos en Ozonoterapia de México y el centro de diseño del ITESM Campus Guadalajara . Se describe el reto de diseñar un equipo que cumpla con las normas internacionales sobre la generación controlada de Ozono para usos médicos y que sea más económico con respecto a los productos que se importan del extranjero. Los resultados muestran la factibilidad de emprender la posibilidad de construir este tipo de equipo en México por la importancia y la alternativa que ofrece el tratamiento de ozonoterapia en una gran cantidad de enfermedades.