Serena Viva

This study aimed to evaluate the reliability of an age estimation method based on the pulp⁄tooth area ratio by assessing intra-and inter-examiner agreement across five observers at different intervals. Using the same X-ray device and technical parameters, 96 digital periapical X-ray images of upper and lower canines were obtained from 28 deceased people in Central America, whose age at death ranged from 19 to 49 years. Excellent and good agreement of results were achieved, and there were no statistically significant differences. The R2 value for upper teeth (54.0%) was higher than the R2 value for lower teeth (45.7%). The highest intraclass correlation coefficient value was 0.995 (0.993-0.997) and the lowest 0.798 (0.545-0.895). Inter-examiner agreement was high with values of 0.975 (0.965-0.983) and 0.927 (0.879-0.955). This method is adequate for assessing age in missing and unidentified people, including victims of mass disasters.

During excavations carried out at the necropolis of San Genesio, taphonomic analysis permitted the detection of a small nucleus of distinctive graves dated to the sixth century and set within the wider context of autochthonous burials. This group was characterized by features such as wooden coffins and tree trunk burials, elements typical of the Germanic cultural milieu. Furthermore, anthropological analysis of the skeletal remains identified a case of Artificial Cranial Deformation (ACD), a distinctive element of Gothic ethnic groups. Individuals with ACD are extremely rare in the Italian archeological record and are related to the period in which the Gothic migrations took place. The subject, buried in a wooden casket, was a male individual with a deformed skull, polytraumatized, and most likely killed in combat, possibly during the Gothic War. This is the first Italian case of an individual with ACD, who died a certified violent death.
The present study, despite the absence of traditional Germanic grave goods, has allowed to hypothesize the existence at San Genesio of an allochthonous group, a theory further supported by strontium isotope analysis.

Pathological conditions in skeletal remains, such as cribra orbitalia and porotic hyperostosis, can be caused by etiologically different forms of anemia: hereditary hemolytic anemias, iron deficiency anemias, megaloblastic anemias, etc. Though not always simple to apply, differential diagnosis of cases of anemia found in the archaeological record is essential to correlate the pathology with the wider environmental and socio-cultural context.
Anthropological analysis of the skeletal sample from the archaeological site of Vetricella showed a very high prevalence of cribra orbitalia and porotic hyperostosis. The differential diagnosis between acquired and congenital anemia is essential in the study of this condition, especially considering the presence of Plasmodium falciparum in this area and its role in the possible subsequent spread of the thalassaemia gene, which was widespread until a few decades ago.
Carbon and nitrogen stable isotope analysis was carried out on a large subset of the skeletal sample, including both anemic and non-anemic individuals, to investigate the nutritional patterns of the Vetricella population in order to verify whether the widespread traces of anemia might be of dietary origin.
Isotope results allowed for the exclusion of the hypothesis of nutritional-deficiency anemia and supported the hypothesis of congenital anemia.

Throughout the ancient Greek, world mass burials are rare. Of the 10,000 excavated burials in the western necropolis of the Greek colony of Himera (649-409), only 16 contained more than one individual; seven of these can in all possibility be traced back to the two battles fought by Himera against the Carthaginians in 480 and 409 BCE. Written sources (Diod. 11.20ff.) state that the Himerans, accompanied by numerous Greek allies, defeated the Carthaginian forces in the first conflict whereas the loss of Greek allied support in the second battle resulted in the city's defeat and subsequent destruction. The anthropological and taphonomical studies of the human remains aim at testing the archaeological hypothesis that individuals interred in the seven mass graves can be related to the two battles. Skeleton density (skeletons per square metre), position, orientation, and conservation levels of the individuals in the mass burials were analysed and compared with a larger sample of 1,000 single burials from the same necropolis along with mass graves in other forensic and archaeological contexts. Sex, age at death, presence of skeletal stress indicators, and perimortal traumas were recorded using standard anthropologi-cal methods. In the case of poorly preserved remains, sex was estimated using site-specific discriminant functions. Although representing catastrophic samples, the results from the seven mass burials all lead to the assumption that the graves can be associated with the interment of warriors fallen in battle. Moreover, burial differences have permitted to distinguish the mass burials dating to 480 BCE from those attributed to 409 BCE.

Assessment of legal age, also known as age of majority, is a controversial issue as there are few body biomarkers or evidence during late adolescence differentiating a subject from being a minor or adult. The third molar was recognized as a suitable site for age examination in late adolescence. We analyzed the development of the left mandibular third molar by the third molar maturity index (I3M) and a specific cut-off value of I3M = 0.08, established by Cameriere et al. in 2008 and used it for discriminating between minors and adult black Africans from Gaborone, Botswana. A final sample of panoramic radiographs (OPTs) of 1294 people (582 males and 712 females) aged between 13 and 23 years was evaluated. The real age decreased as I3M gradually increased. There was no statistically significant difference in the third molar development evaluated using I3M between males and females (p > 0.05) across different I3M classes. Results of 2 × 2 contingency tables for different cut-off values indicated that I3M = 0.08 was useful in discriminating between adults and minors. Precisely, for I3M = 0.08, the values of accuracy or overall fraction of correctly classified were 0.91 in males with a 95 % confidence interval (95 % CI) of 0.88 to 0.93 and 0.92 (95 % CI, 0.90 to 0.93) in females. Values of sensitivity of the test or the proportion of participants being 18 years and older were 0.88 (95 % CI, 0.87 to 0.90) in males and 0.88 (95 % CI, 0.90 to 0.93) in females, while values of specificity or proportion of individuals younger than 18 who have I3M <0.08 were 0.94 (95 % CI, 0.91 to 0.96) in males and 0.96 (95 % CI, 0.94 to 0.98) in females. Positive predictive values of the test, where the participants whose I3M <0.08 were adults, were 0.94 (95 % CI 0.91 to 0.96) in males and 0.97 (95 % CI, 0.94 to 0.98) in females, while negative predictive values of the test, where the participants whose I3M was ≥0.08 were minors, were 0.88 (95 % CI 0.85 to 0.90) in males and 0.97 (95 % CI, 0.94 to 0.98) in females. The likelihood ratios of the positive test (LR+) were 13.67 (95 % CI, 9.21 to 21.02) in males and 23.73 (95 % CI, 14.20 to 42.28) in females, while likelihood ratios of the negative test (LR-) were 0.12 (95 % CI 0.10 to 0.16) in males and 0.12 (95 % CI, 0.11 to 0.15) in females. Bayes post-test probabilities, p, were 0.94 (95 % CI 0.90 to 0.98) in males and 0.97 (95 %CI, 0.93 to 1.00) in females. These results indicate with high accuracy that I3M may be a useful alternative method in legal and forensic practice to discriminate individuals of black African origin who are around the legal adult age of 18 years in Botswana. Further studies should address the usefulness of this method and specific cut-off for different adolescent populations.

The estimation of an individual's age at the time of death is one of the most important components in anthropological studies and is the basis for demographic studies on ancients. However, the different methods commonly used in anthropology for adult age estimation at death provide results with a high level of uncertainty. The consequence is the inability to develop demographic studies with a good degree of reliability. A non-destructive method currently available is the analysis of the apposition of secondary dentine on which Cameriere's method is based.

This volume consists of a series of archaeological scientific articles by colleagues who have had the opportunity to share their work with Stefano Vassallo, being able, in this way, to appreciate the professional and, even more, human qualities with which he has carried out his work in his more than thirty-year career.