Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar[1] which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.
The commonly known types are:
- Single diffusion in one dimension (Oudin procedure)
- Double diffusion in one dimension (Oakley Fulthorpe procedure)
- Single diffusion in two dimensions (radial immunodiffusion or Mancini method)[2]
- Double diffusion in two dimensions (Ouchterlony double immunodiffusion)[3]
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Radial Immunodiffusion (Mancini Technique) (FL-Immuno/57)
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Double immunodiffusion (Ouchterlony Double Diffusion) (FL-Immuno/58)
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Radial immunodiffusion
Transcription
Notes
- ^ Coleman, R. M.; Kaufman, L. (1972). "Use of the Immunodiffusion Test in the Serodiagnosis of Aspergillosis". Applied Microbiology. 23 (2): 301–308. doi:10.1128/am.23.2.301-308.1972. PMC 380335. PMID 4622826.
- ^ "Radial Immunodiffusion". Edvotek, Inc. 2017. Archived from the original (photograph) on 2017-08-07. Retrieved 2017-08-07. Photograph of precipitin circles in a Petri dish during radial immunodiffusion.
- ^ "Diffusion Patterns". Immunodiffusion principles and application. Archived from the original on 2019-12-11. Retrieved 2017-05-19. Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity.
External links
- Immunodiffusion at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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This page was last edited on 4 April 2023, at 18:14