Temporins are a family of peptides isolated originally from the skin secretion of the European red frog, Rana temporaria.[1] Peptides belonging to the temporin family have been isolated also from closely related North American frogs, such as Rana sphenocephala.[2]
Discovery
In 1996, the skin of the Rana temporaria was screened into a cDNA library, discovering three peptide precursors, known as Temporin B, Temporin G, and Temporin H. Once discovered, the three peptide, along with other temporins found in the sample, were separated from secretions of the frog's skin. Biological assays were performed, revealing that all temporins, in exception of temporin D and H, exhibited antibacterial activity. It was soon discovered that other species of frog also possess temporal, resulting in the discovery of more than 150 peptides from the temporin family. Some of the genera of frogs include the Amolops, Hylarana, Lithobathes, Odorrana, Pelophylax, Rana, and Hylarana.[3]
Clinical Uses
Temporins are a family of antimicrobial peptides (AMPs), which are highly targetable toward Gram-positive bacteria, allowing it to penetrate the cell wall and damage the inner membrane of the bacteria. These proteins are also capable of killing specific cancer cells when they are locally administered to a tumor, making it a good anticancer drug.[4]
References
- ^ Simmaco M; Mignogna G; Canofeni S; Miele R; Mangoni ML; Barra D (Dec 15, 1996). "Temporins, antimicrobial peptides from the European red frog Rana temporaria". Eur J Biochem. 242 (3): 788–792. doi:10.1111/j.1432-1033.1996.0788r.x. PMID 9022710.
- ^ Holden, Whitney; Reinert, Laura K; Hanlon, Shane M; Parris, Matthew J; Rollins-Smith, Louise A (January 2015). "Development of antimicrobial peptide defenses of southern leopard frogs, Rana sphenocephala, against the pathogenic chytrid fungus, Batrachochytrium dendrobatidis". Developmental & Comparative Immunology. 48 (1): 65–75. doi:10.1016/j.dci.2014.09.003. PMID 25218643. Retrieved 16 June 2021.
- ^ D'Andrea, Luca Domenico; Romanelli, Alessandra (12 March 2023). "Temporins: Multifunctional Peptides from Frog Skin" (PDF). International journal of molecular sciences.
- ^ Antony, Anet; Purayil, Anupama Kizhakke; Olakkaran, Shilpa; Dhannura, Shweta; Shekh, Shamasoddin; Gowd, Konkallu Hanumae; Gurushankara, Hunasanahally Puttaswamygowda (2024). "Antimicrobial and antitumor properties of anuran peptide temporin-SHf induce apoptosis in A549 lung cancer cells" (PDF). Amino Acids. 56 (1): 12. doi:10.1007/s00726-023-03373-3. PMC 10847208. PMID 38319435.
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