Search Results (66)

Glioblastoma, a formidable brain tumor characterized by dysregulated NAD metabolism, poses a significant therapeutic challenge. The NAMPT inhibitor FK866, which induces NAD depletion, has shown promise in controlling tumor proliferation and modifying the tumor microenvironment. However, the clinical efficacy of FK866 as a single drug therapy for glioma is limited. In this study, we aim to disrupt NAD metabolism using fluorinated NAD precursors and explore their synergistic effect with FK866 in inducing cytotoxicity in glioblastoma cells. The synthesized analogue of nicotinamide riboside (NR), ara-F nicotinamide riboside (F-NR), inhibits nicotinamide ribose kinase (NRK) activity in vitro, reduces cellular NAD levels, and enhances FK866’s cytotoxicity in U251 glioblastoma cells, indicating a collaborative impact on cell death. Metabolic analyses reveal that F-NR undergoes conversion to fluorinated nicotinamide mononucleotide (F-NMN) and other metabolites, highlighting the intact NAD metabolic pathway in glioma cells. The activation of SARM1 by F-NMN, a potent NAD-consuming enzyme, is supported by the synergistic effect of CZ-48, a cell-permeable SARM1 activator. Temporal analysis underscores the sequential nature of events, establishing NAD depletion as a precursor to ATP depletion and eventual massive cell death. This study not only elucidates the molecular intricacies of glioblastoma cell death but also proposes a promising strategy to enhance FK866 efficacy through fluorinated NAD precursors, offering potential avenues for innovative therapeutic interventions in the challenging landscape of glioblastoma treatment. Full article

Understanding the spatial heterogeneity of bird community distribution within urban greenways is crucial for optimizing ecological functions and supporting urban biodiversity. While ecological corridors enhance connectivity and biodiversity, specific mechanisms by which landscape composition and configuration influence bird diversity remain unclear. This study examines bird community distribution along the Qingshan Lake Greenway in Hangzhou during autumn and winter, using 17 observation points across wetlands, forests, and mixed habitats. The key findings indicate that wetlands have significantly higher species richness compared to forests and mixed habitats, underscoring wetlands’ critical role in supporting diverse bird communities. Greenways primarily facilitate short-distance bird movement with limited permeability between habitats. Additionally, greenway effectiveness in enhancing bird diversity depends heavily on design and structural characteristics. This research highlights the necessity of incorporating microhabitat design and diverse habitat configurations in greenway planning to enhance ecological connectivity and biodiversity. It provides essential insights for urban planners and ecologists, emphasizing detailed landscape composition and configuration analyses. Future research should include year-round studies and advanced ecological monitoring technologies to validate and expand these findings, ultimately contributing to more effective urban biodiversity conservation and sustainable development strategies. Full article

Given the increasing aging population and the rising prevalence of musculoskeletal diseases due to obesity and injury, urgent research is needed to formulate new treatment alternatives, as current options remain inadequate. Viruses can exacerbate arthritis and worsen symptoms in patients with pre-existing osteoarthritis. Over the past decade, the chikungunya virus (CHIKV) has emerged as a significant public health concern, especially in Asia and South America. Exploring natural products, such as berberine, has shown promise due to its anticatabolic, antioxidative, and anti-inflammatory effects. However, berberine’s low stability and bioavailability limit its efficacy. We hypothesized that encapsulating berberine into a proniosome gel, known for its ease of preparation and stability, could enhance its bioavailability and efficacy when applied topically, potentially treating CHIKV infection. Our investigation focused on how varying berberine loads and selected excipients in the proniosome gel influenced its physical properties, stability, and skin permeability. We also examined the biological half-life of berberine in plasma upon topical administration in mice to assess the potential for controlled and sustained drug release. Additionally, we analyzed the antioxidant stress activity and cell viability of HaCaT keratinocytes and developed a lipopolysaccharide-stimulated cell culture model to evaluate anti-inflammatory effects using pro-inflammatory cytokines. Overall, the research aims to transform the treatment landscape for arthritis by leveraging berberine’s therapeutic potential. Full article

Increasing soil contamination with nickel (Ni) and copper (Cu) is a growing environmental concern, adversely affecting ecosystems and the survival of both plants and animals. This study investigated the morphological and physiological responses of Euphorbia marginata Pursh seedlings to varying concentrations of Ni and Cu over a 45-day period. The findings revealed that low concentrations of Ni and Cu enhanced morphological indexes, root indexes, biomass, and photosynthetic pigment content of E. marginata, while high concentrations inhibited these parameters. Compared to the control, Ni and Cu stresses induced membrane peroxidation, increased cell membrane permeability, and inhibited the synthesis of soluble proteins and proline in the leaves. The seedlings demonstrated an ability to mitigate Ni and Cu toxicity by increasing soluble sugar content and enhancing the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Notably, E. marginata exhibited a higher capacity for Cu²⁺ enrichment and translocation compared to Ni²⁺. Combined Ni and Cu treatments reduced the maximum enrichment and translocation levels of both metals in E. marginata. This study highlights the superior tolerance of E. marginata to Ni and Cu stresses and elucidates the mechanisms underlying its response, providing a theoretical basis for the use of landscape plants in the remediation of heavy-metal-contaminated soils. Full article

Building information modeling (BIM) has been used by the architectural and engineering disciplines to streamline the building design, construction, and management process, but there has been much more limited experience in extending the application to landscape design and implementation. This study integrated BIM software (Autodesk InfraWorks 2024.1) with a dynamic, process-oriented, conceptual hydrologic/hydraulic model (PCSWMM 2023, version 7.6.3665) to enhance the analytical tools for sustainable landscape design. We illustrate the model integration through a case study that links an existing nature-based solution (NbS) development, the PTT Metro Forest Park, Bangkok, Thailand, with theoretical new-build NbS for an adjacent property. A BIM school building was virtually situated on an empty lot beside the Metro Forest Park and seven NbS scenarios were run with design storms having 2-year, 5-year, and 100-year return intervals. The combination of a rain garden, permeable pavement, a retention pond, and a green roof was effective in sustainably managing runoff from the theoretical new-build site discharging to the Metro Forest. NbS design characteristics such as rain garden substrate depth and green roof area were optimized using the hydrologic/hydraulic model. Model results showed that even with the 100-year rainfall event, the existing Metro Forest pond storage capacity was sufficient so that flooding on the property would not occur. The consideration of connectivity between NbS features is facilitated by the modeling approach, which is important for NbS planning and assessment at a regional scale. Full article

The primacy of endozoochory for the maintenance and expansion of many woody plant populations is well known, but seed dispersal is not well understood for most species. This is especially true for rare species, where small population size and low fruit production can limit field- or observation-based experiments. Additionally, the effect of environmental heterogeneity on disperser movement is rarely investigated but has been shown to improve estimates of plant population spatial patterns and dynamics. We used simulation experiments to explore the effects of environmental heterogeneity and disperser movement on Lindera subcoriacea seed dispersal, a rare shrub from the southeastern United States with avian-dispersed seeds. Our experiments incorporated environmental heterogeneity and simulated disperser movement for five bird species, based on either landscape permeability or straight path rules. We anticipated that permeability-based movement would result in greater dispersal distances and seed dispersal effectiveness, which characterizes both quantity and quality. Generally, we did not find differences in seed dispersal between permeability and straight path experiments. However, we did find that permeability-based experiments had greater deposition into suitable habitat during flight (23 vs. 1%). These rare but longer distance depositions may be especially important for plants that are influenced by gap or interpopulation dynamics. We also found consistently greater dispersal into high quality habitats regardless of disperser species in permeability experiments, implying that incorporating species-specific assessments of landscape utilization (occupancy) could influence the effectiveness of seed dispersal. Our study suggests that including environmental heterogeneity in seed dispersal models can provide additional insights not provided by avian parameters (e.g., gut capacity, seed retention time, and flight speed) commonly used to inform dispersal models. Full article

This study investigates how permeable and cool pavements, green roofs, and living walls affect microclimatic conditions and buildings’ energy consumption in an arid urban setting: Shiraz. The study aims to evaluate the role of green infrastructure in mitigating urban heat island effects and enhancing outdoor conditions. By utilizing environmental modeling tools, specifically the ENVI-met 5.1.1 and Design Builder 7.0.2 software, a comprehensive analysis was conducted across various scenarios during both the summer and winter seasons. The results show that permeable pavements with 80% grass coverage reduced the mean average air temperature by 1.18 °C in summer mornings compared to the reference case. In both the summer and winter scenarios, the living wall intervention consistently emerged as the most effective strategy, showcasing substantial reductions in cooling consumption, CO₂ emissions, and electricity consumption. With a 25% reduction in cooling consumption, a 14.7% decrease in CO₂ emissions, and an impressive 53.4% decline in electricity consumption, the living wall excelled in its environmental impact, and it stands out for its substantial electricity savings. While the green roof and permeable pavement scenarios demonstrated more modest gains, their integration could offer a synergistic solution, warranting further exploration for optimized energy efficiency and environmental sustainability. These findings indicate the intrinsic connection between sustainable landscaping strategies and their influence on urban microclimate and building energy efficiency. Full article

Mongolian oak (Quercus mongolica) is a common building material and landscaping tree species in northern China, with significant economic and ecological value. Its seedling growth is inhibited by high light intensity, but the mechanism by which light stress affects the growth and development of its seedlings remains unclear. In this study, we investigated the phenotypes, physiological processes, and molecular responses of 3-year-old Mongolian oak seedlings under different light treatments: full light (Sck), light shading (S1; 40% light), moderate shading (S2; 20% light), and severe shading (S3; 3% light). Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings. The S1 and S2 treatments also promoted seedling height and diameter growth and resulted in lower degrees of membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activity. In contrast, severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate, and exhibiting higher degrees of membrane lipid peroxidation and cell membrane permeability. Shading treatments (S1 and S2) alleviated the negative effects of strong light on the growth and development of Mongolian oak seedlings, with the S2 treatment having the greatest effect. However, severe shading (S3) inhibited growth and development. A total of 3726 differentially expressed genes (DEGs) were detected in leaves under different shading treatments in RNA sequencing analysis. Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively. The different shading treatments determined common expression regulation pathways, including carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transduction. Shading induced increases in gene expression levels in light harvesting complexes, which are related to changes in gene expression in the photosynthetic system, leading to changes in photosynthetic physiology. The expression levels of genes related to reactive oxygen species signal perception and activation enzymes were upregulated in Sck. Together, these findings revealed the response mechanisms of Mongolian oak seedlings to different shading levels at the physiological and molecular levels, providing a scientific basis and technical support for the cultivation and large-scale production of Mongolian oak seedlings. Full article

The extreme drought in Europe in 2022 also hit hard the Great Hungarian Plain. In this short overview article, we summarize the natural environmental conditions of the region and the impact of river control works on the water-retention capacity of the landscape. In this respect, we also review the impact of intensive agricultural cultivation on soil structure and on soil moisture in light of the meteorological elements of the 2022 drought. The most important change is that the soil stores much less moisture than in the natural state; therefore, under the meteorological conditions of summer 2022, the evapotranspiration capacity was reduced. As a result, the low humidity in the air layers above the ground is not sufficient to trigger summer showers and thunderstorms associated with weather fronts and local heat convection anymore. Our proposed solution is to restore about one-fifth of the area to the original land types and usage before large-field agriculture. Low-lying areas should be transformed into a mosaic-like landscape with good water supply and evapotranspiration capacity to humidify the lower air layers. Furthermore, the unfavorable soil structure that has resulted from intensive agriculture should also be converted into more permeable soil to enhance infiltration. Full article

The low FODMAP (fermentable oligosaccharide, disaccharide, monosaccharide, and polyol) diet is a beneficial therapeutic approach for patients with irritable bowel syndrome (IBS). However, how the low FODMAP diet works is still not completely understood. These mechanisms encompass not only traditionally known factors such as luminal distension induced by gas and water but also recent evidence on the role of FOMAPs in the modulation of visceral hypersensitivity, increases in intestinal permeability, the induction of microbiota changes, and the production of short-chain fatty acids (SCFAs), as well as metabolomics and alterations in motility. Although most of the supporting evidence is of low quality, recent trials have confirmed its effectiveness, even though the majority of the evidence pertains only to the restriction phase and its effectiveness in relieving abdominal bloating and pain. This review examines potential pathophysiological mechanisms and provides an overview of the existing evidence on the effectiveness of the low FODMAP diet across various IBS subtypes. Key considerations for its use include the challenges and disadvantages associated with its practical implementation, including the need for professional guidance, variations in individual responses, concerns related to microbiota, nutritional deficiencies, the development of constipation, the necessity of excluding an eating disorder before commencing the diet, and the scarcity of long-term data. Despite its recognized efficacy in symptom management, acknowledging these limitations becomes imperative for a nuanced comprehension of the role of a low FODMAP diet in managing IBS. By investigating its potential mechanisms and evidence across IBS subtypes and addressing emerging modulations alongside limitations, this review aims to serve as a valuable resource for healthcare practitioners, researchers, and patients navigating the intricate landscape of IBS. Full article

This work presents a methodology for the hydrological characterization of natural and urban landscapes, focusing on accurate estimations of infiltration capacity and runoff characteristics. By combining existing methods from the literature, we created a systemic process that integrates satellite-based vegetation maps, topography, and soil permeability data. This process generates a detailed vegetation classification and slope-corrected composite curve number (CN_cα) map using information at the subpixel level, which is crucial for estimating excess runoff during intense precipitation events. The algorithm designed with this methodology is automated and utilizes freely accessible multispectral imagery. Leveraging the vegetation–impervious–soil (V-I-S) model, it is assumed that land cover comprises V-I-S components at each pixel. Automated Music and spectral Separability-based Endmember Selection is employed on a generic spectral library to obtain the most relevant V-I-S endmember spectra for a particular image, which is then employed in multiple endmember spectral mixture analysis to obtain V-I-S fraction maps. The derived fractions are utilized in combination with the Normalized Difference Vegetation Index and the Modified Normalized Difference Water Index to adapt the CN_cα map to different seasons and climatic conditions. The methodology was applied to Esch-sur-Alzette, Luxembourg, over a four-year period to validate the methodology and quantify the increase in the impervious surface area in the commune and the relationship with the runoff dynamics. This approach provides valuable insights into infiltration and runoff dynamics across diverse temporal and geographic ranges. Full article

Geogas-carried metal prospecting, an integral part of deep-penetrating geochemistry, is potentially effective in the geochemical exploration of concealed ore deposits. However, its principles and applicability remain controversial. This study summarizes and discusses the progress in geogas-carried metal prospecting in China. The method comprises three constituents: geogas, nanoparticles, and their vertical transportation. Researchers have failed to determine the exact contributions of different sources of geogas. Studies on Pb isotopes, rare earth element patterns of geogas, the comparisons between metals in soil, geogas, and ore geochemistry, and characteristics of nanoscale metals in earthgas (NAMEG), confirmed the relationship between NAMEG and concealed ore deposits. A statistical analysis of field experiments and applications showed that geogas-carried metal prospecting is applicable for the geochemical exploration of magmatic and hydrothermal Cu, Au, Zn, Pb, U, Sn, and Ag deposits and is suitable for most geochemical landscapes except deserts and cold swamps. Finally, genetic models of NAMEG anomalies were constructed. High-permeability migration channels are critical in the formation of NAMEG anomalies over concealed ore deposits. Future work entails applying geogas-carried metal prospecting to certain types of ore deposits and geochemical landscapes and studying NAMEG to provide quantitative information for targeting concealed ore deposits. Full article

The consumption of large amounts of dietary fats and pregnancy are independent factors that can promote changes in gut permeability and the gut microbiome landscape. However, there is limited evidence regarding the impact of pregnancy on the regulation of such parameters in females fed a high-fat diet. Here, gut permeability and microbiome landscape were evaluated in a mouse model of diet-induced obesity in pregnancy. The results show that pregnancy protected against the harmful effects of the consumption of a high-fat diet as a disruptor of gut permeability; thus, there was a two-fold reduction in FITC–dextran passage to the bloodstream compared to non-pregnant mice fed a high-fat diet (p < 0.01). This was accompanied by an increased expression of gut barrier-related transcripts, particularly in the ileum. In addition, the beneficial effect of pregnancy on female mice fed the high-fat diet was accompanied by a reduced presence of bacteria belonging to the genus Clostridia, and by increased Lactobacillus murinus in the gut (p < 0.05). Thus, this study advances the understanding of how pregnancy can act during a short window of time, protecting against the harmful effects of the consumption of a high-fat diet by promoting an increased expression of transcripts encoding proteins involved in the regulation of gut permeability, particularly in the ileum, and promoting changes in the gut microbiome. Full article

Globally, groundwater-influenced ecosystems (GIEs) are increasingly vulnerable to groundwater extraction and land use practices. Groundwater supports these ecosystems by providing inflow, which can maintain water levels, water temperature, and the chemistry necessary to sustain the biodiversity that they support. Many aquatic systems receive groundwater as a portion of baseflow, and in some systems, the connection with groundwater is significant and important to the system’s integrity and persistence. There is a lack of information about where these systems are found and their relationships with environmental conditions in the surrounding landscape. Additionally, groundwater management for human use often does not address maintaining the ecological functions of GIEs. We used correlative distribution modeling methods (GLM, GAM, MaxEnt, Random Forest) to predict landscape-scale habitat suitability for GIEs in two ecologically distinct ecoregions (EPA Level II ecoregions: Atlantic Highlands and Mixed Wood Plains) in the northeastern United States. We evaluated and combined the predictions to create ensemble models for each ecoregion. The accuracy of the ensemble models was 75% in the Atlantic Highlands and 86% in the Mixed Wood Plains. In the Mixed Wood Plains, hydric soil, surface materials, and soil permeability were the best predictors of GIE presence, whereas hydric soil, topographic wetness index, and elevation were the best predictors of GIE presence in the Atlantic Highlands. Approximately 1% of the total land area in each ecoregion was predicted to be suitable for GIEs, highlighting that there likely is a small proportion of the landscape occupied by these systems. Full article

The growing relevance of video games in the social landscape requires an in-depth evaluation of the bidirectional influences of these adjacent contexts, among which the educational and cultural contexts stand out. This permeability allows the study of the cultural aspects surrounding the production and use of video games as tools for cultural dissemination. Our research enunciates, recognises, analyses and evaluates the impact of these tools with the aim of being taken into consideration by the scientific community and the general population, who most frequently consume, use and socialise through video games around the world. Based on this, a systematic literature review (SLR) was carried out using the Prisma 2020 Protocol, using the WOS and SCOPUS databases as documentary sources, and the selection of research articles that explore the cultural aspects of video games according to pre-established criteria. The final sample (N = 45) was subjected to a study that posed 12 research questions around which this work is structured. As a result, it is evident that video games have a direct impact on the transmission of culture. Patterns and agents of cultural representation, transmission and communication were identified, allowing researchers to evaluate the impact of video games on culture and to approach this object of study from an academic perspective, helping to open up other lines of research related to the influence of video games on the assimilation of culture, the learning of relevant issues related to it or the transmission of specific cultural elements. Full article