Abstract
We describe the isolation and characterization of NSD3, the third member of a gene family including Nsd1 and NSD2. Murine Nsd1 was isolated in a search for proteins that interact with the ligand-binding domain of retinoic acid receptor alpha. NSD2 (also known as WHSC1 and MMSET) is located in the Wolf-Hirschhorn syndrome (WHS) critical region on 4p16.3 and is involved in multiple myeloma with t(4;14) translocations. The proteins Nsd1, NSD2, and NSD3 are highly similar within a block of about 700 amino acids. This block contains several conserved domains, such as the SET domain and the PHD finger, present in proteins involved in development and/or chromatin reorganization. The NSD3 gene consists of an 8.5-kb transcript composed of 23 coding exons and spans >90 kb of genomic DNA. NSD3 maps to chromosome band 8p12 and is amplified in several tumor cell lines and primary breast carcinomas.
Copyright 2001 Academic Press.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Binding Sites
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Blotting, Northern
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Breast Neoplasms / genetics*
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Breast Neoplasms / pathology
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Carrier Proteins / genetics*
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Chromosome Mapping
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Chromosomes, Human, Pair 8 / genetics*
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Exons
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Female
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Gene Amplification
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Gene Expression
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Genes / genetics
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Histone-Lysine N-Methyltransferase
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Poly A / genetics
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Protein Isoforms / genetics
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RNA / genetics
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RNA / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
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Tumor Cells, Cultured
Substances
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Carrier Proteins
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DNA, Complementary
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Nuclear Proteins
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Protein Isoforms
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Poly A
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RNA
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Histone-Lysine N-Methyltransferase
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NSD3 protein, human
Associated data
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GENBANK/AJ295990
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GENBANK/AJ295991
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GENBANK/AJ295992