Visalli, G.; Facciolà, A.; Currò, M.; Laganà, P.; La Fauci, V.; Iannazzo, D.; Pistone, A.; Di Pietro, A. Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes. Int. J. Environ. Res. Public Health2019, 16, 792.
Visalli, G.; Facciolà, A.; Currò, M.; Laganà, P.; La Fauci, V.; Iannazzo, D.; Pistone, A.; Di Pietro, A. Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes. Int. J. Environ. Res. Public Health 2019, 16, 792.
Visalli, G.; Facciolà, A.; Currò, M.; Laganà, P.; La Fauci, V.; Iannazzo, D.; Pistone, A.; Di Pietro, A. Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes. Int. J. Environ. Res. Public Health2019, 16, 792.
Visalli, G.; Facciolà, A.; Currò, M.; Laganà, P.; La Fauci, V.; Iannazzo, D.; Pistone, A.; Di Pietro, A. Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes. Int. J. Environ. Res. Public Health 2019, 16, 792.
Abstract
Human exposure to carbon nanotubes (CNTs) can cause health issues due to their chemical–physical features and biological interactions. These nanostructures cause oxidative stress, also due to endogenous ROS production, which increases following mitochondrial impairment. The aim of this in vitro study was to assess the health effects, due to mitochondrial dysfunction, caused by a sub-chronic exposure to a non-acutely toxic dose of multi walled CNTs (raw and functionalised). The A549 cells were exposed to MWCNTs (2 µg mL-1) for 36 days. Periodically, cellular dehydrogenases, pyruvate dehydrogenase kinase 1 (PDK1), cytochrome c release, permeability transition pore (mPTP) opening, transmembrane potential (Δψ m), apoptotic cells, and intracellular ROS were measured. The results, compared to untreated cells and to positive control formed by cells treated with MWCNTs (20 µg mL-1), highlighted the efficiency of homeostasis to counteract ROS overproduction, but a restitutio ad integrum of mitochondrial functionality was not observed. Despite the tendency to restore, the mitochondrial impairment persisted. Overall, the results underlined the tissue damage that can arise following sub-chronic exposure to MWCNTs.
Keywords
MWCNTs; oxidative stress; mitochondria
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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