Version 1
: Received: 29 August 2019 / Approved: 30 August 2019 / Online: 30 August 2019 (08:22:58 CEST)
How to cite:
Tosheva, K. L.; Yuan, Y.; Pereira, P. M.; Culley, S.; Henriques, R. Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints2019, 2019080319. https://doi.org/10.20944/preprints201908.0319.v1
Tosheva, K. L.; Yuan, Y.; Pereira, P. M.; Culley, S.; Henriques, R. Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints 2019, 2019080319. https://doi.org/10.20944/preprints201908.0319.v1
Tosheva, K. L.; Yuan, Y.; Pereira, P. M.; Culley, S.; Henriques, R. Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints2019, 2019080319. https://doi.org/10.20944/preprints201908.0319.v1
APA Style
Tosheva, K. L., Yuan, Y., Pereira, P. M., Culley, S., & Henriques, R. (2019). Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints. https://doi.org/10.20944/preprints201908.0319.v1
Chicago/Turabian Style
Tosheva, K. L., Siân Culley and Ricardo Henriques. 2019 "Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy" Preprints. https://doi.org/10.20944/preprints201908.0319.v1
Abstract
Super-Resolution Microscopy enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.