Version 1
: Received: 5 January 2020 / Approved: 5 January 2020 / Online: 5 January 2020 (17:22:10 CET)
How to cite:
Roman, B.; Gomez, P.; Pico, B.; Die, J. V. The NBS-LRR Gene Class is a Small Family in Cucurbita pepo. Preprints2020, 2020010048. https://doi.org/10.20944/preprints202001.0048.v1
Roman, B.; Gomez, P.; Pico, B.; Die, J. V. The NBS-LRR Gene Class is a Small Family in Cucurbita pepo. Preprints 2020, 2020010048. https://doi.org/10.20944/preprints202001.0048.v1
Roman, B.; Gomez, P.; Pico, B.; Die, J. V. The NBS-LRR Gene Class is a Small Family in Cucurbita pepo. Preprints2020, 2020010048. https://doi.org/10.20944/preprints202001.0048.v1
APA Style
Roman, B., Gomez, P., Pico, B., & Die, J. V. (2020). The NBS-LRR Gene Class is a Small Family in <em>Cucurbita pepo</em>. Preprints. https://doi.org/10.20944/preprints202001.0048.v1
Chicago/Turabian Style
Roman, B., Belen Pico and Jose V. Die. 2020 "The NBS-LRR Gene Class is a Small Family in <em>Cucurbita pepo</em>" Preprints. https://doi.org/10.20944/preprints202001.0048.v1
Abstract
Although Cucurbita pepo is one of the most variable species of the plant kingdom, Zucchini morphotype has undergone intensive breeding that has led to a narrow genetic base making the crop vulnerable to pest and diseases. This vulnerability makes the knowledge of resistance genes of utmost importance. In this study, a data mining search of Zucchini summer squash genome database was conducted to identify and annotate members of the NBS-encoding gene family. In order to characterize the retrieved genes in detail, they have been studied in the bases of phylogenetic relationships, structural diversity, conserved protein motifs, gene duplications and promoter region analysis. Our study shows that the NBS-encoding gene family is relatively small in Zucchini (34 members, which are separated into non-TIR- and TIR-NBS-LRR subfamilies) with a significantly lower number of R-genes than in other species. Duplications have not played a major role in the expansion of this type of genes in C. pepo. Among the cis-regulatory elements presented in these sequences, six motifs are over-represented. These elements were reported to be involved in pathogens or plant stress induced responses. These results will contribute to the identification, isolation and characterization of candidate R-genes, thereby providing insight into NBS gene family evolution in the species.
Keywords
cis-regulatory element; data mining; NBS-LRR resistance genes; Zucchini
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.