Version 1
: Received: 21 December 2021 / Approved: 22 December 2021 / Online: 22 December 2021 (12:14:09 CET)
How to cite:
Lim, E. H.; Mun, S.-K.; Kim, J.-J.; Chang, D.-J.; Yee, S.-T. Anti-Inflammatory Effects of Phlebia sp. Extract in Lipopolysaccharide-stimulated RAW 264.7 Macrophages. Preprints2021, 2021120361. https://doi.org/10.20944/preprints202112.0361.v1
Lim, E. H.; Mun, S.-K.; Kim, J.-J.; Chang, D.-J.; Yee, S.-T. Anti-Inflammatory Effects of Phlebia sp. Extract in Lipopolysaccharide-stimulated RAW 264.7 Macrophages. Preprints 2021, 2021120361. https://doi.org/10.20944/preprints202112.0361.v1
Lim, E. H.; Mun, S.-K.; Kim, J.-J.; Chang, D.-J.; Yee, S.-T. Anti-Inflammatory Effects of Phlebia sp. Extract in Lipopolysaccharide-stimulated RAW 264.7 Macrophages. Preprints2021, 2021120361. https://doi.org/10.20944/preprints202112.0361.v1
APA Style
Lim, E. H., Mun, S. K., Kim, J. J., Chang, D. J., & Yee, S. T. (2021). Anti-Inflammatory Effects of Phlebia sp. Extract in Lipopolysaccharide-stimulated RAW 264.7 Macrophages. Preprints. https://doi.org/10.20944/preprints202112.0361.v1
Chicago/Turabian Style
Lim, E. H., Dong-Jo Chang and Sung-Tae Yee. 2021 "Anti-Inflammatory Effects of Phlebia sp. Extract in Lipopolysaccharide-stimulated RAW 264.7 Macrophages" Preprints. https://doi.org/10.20944/preprints202112.0361.v1
Abstract
Lichens are a life form in which algae and fungi have a symbiotic relationship. A lichen has various biological activities, including anti-inflammatory and anti-proliferative activities. Inflammation is a response caused by various factors, such as infection by pathogens or tissue damage; excessive reactions can contribute to the etiology of chronic diseases, such as asthma, brain damage, and serious tissue damage. This study demonstrates the anti-inflammatory effect of ethyl acetate extract from Phlebia sp. on NF-κB and AP-1 pathways in the lipopolysaccharide-treated RAW 264.7 cell. Especially, Phlebia sp. extract inhibits the phosphorylation of AP-1 signaling (c-Fos and c-Jun) and its upstream MKK/MAPKs (MKK4, MKK7 and JNK), which induced a decrease in the production of the inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and granulocyte-macrophage colony-stimulating factor (GM-CSF) in downstream of AP-1 signaling. Furthermore, Phlebia sp. extract inhibited the production of final inflammatory effector molecules involved in AP-1 signaling, including nitric oxide (NO) and prostaglandin E2 (PGE2). Here, we report that Phlebia sp. extract has the potential to be developed as an anti-inflammatory agent.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.