Version 1
: Received: 4 April 2023 / Approved: 5 April 2023 / Online: 5 April 2023 (10:10:59 CEST)
Version 2
: Received: 13 April 2023 / Approved: 18 April 2023 / Online: 18 April 2023 (05:04:07 CEST)
How to cite:
Nascimento, L. S. D.; Rocha, D. C. D. C.; Souza, A. H.; Pimentel, R. D. S. R.; Costa, D. D. C.; Cruz, M. I. C. D.; Tavares, H. A.; Carneiro, B. S.; Silva, M. C. D. M. Microbiological Quality of Oysters From an Estuary in Northeast Pará, Brazil, Before and After Purification Process. Preprints2023, 2023040053. https://doi.org/10.20944/preprints202304.0053.v2
Nascimento, L. S. D.; Rocha, D. C. D. C.; Souza, A. H.; Pimentel, R. D. S. R.; Costa, D. D. C.; Cruz, M. I. C. D.; Tavares, H. A.; Carneiro, B. S.; Silva, M. C. D. M. Microbiological Quality of Oysters From an Estuary in Northeast Pará, Brazil, Before and After Purification Process. Preprints 2023, 2023040053. https://doi.org/10.20944/preprints202304.0053.v2
Nascimento, L. S. D.; Rocha, D. C. D. C.; Souza, A. H.; Pimentel, R. D. S. R.; Costa, D. D. C.; Cruz, M. I. C. D.; Tavares, H. A.; Carneiro, B. S.; Silva, M. C. D. M. Microbiological Quality of Oysters From an Estuary in Northeast Pará, Brazil, Before and After Purification Process. Preprints2023, 2023040053. https://doi.org/10.20944/preprints202304.0053.v2
APA Style
Nascimento, L. S. D., Rocha, D. C. D. C., Souza, A. H., Pimentel, R. D. S. R., Costa, D. D. C., Cruz, M. I. C. D., Tavares, H. A., Carneiro, B. S., & Silva, M. C. D. M. (2023). Microbiological Quality of Oysters From an Estuary in Northeast Pará, Brazil, Before and After Purification Process. Preprints. https://doi.org/10.20944/preprints202304.0053.v2
Chicago/Turabian Style
Nascimento, L. S. D., Bruno Santana Carneiro and Monica Cristina de Moraes Silva. 2023 "Microbiological Quality of Oysters From an Estuary in Northeast Pará, Brazil, Before and After Purification Process" Preprints. https://doi.org/10.20944/preprints202304.0053.v2
Abstract
This study evaluated the microbiological quality of farm oysters, cultivated in an estuary in northeastern Pará, Brazil, before and after purification process. Water and oyster samples were collected in June, August, October, and December 2019. Oysters underwent purification for up to 36 h in a small-scale adapted debugger. Microbiological analyses were performed in oysters and water depuration collected at different times of purification (0 h pre-treatment, 6 h, 12 h, 2 4h, and 36 h), using the multiple tube technique, to determine the most probable number (MPN) of Escherichia coli, thermotolerant coliforms - TTC, and total coliforms - TC. Bacterial isolation was used to determine the presence of Salmonella spp. Before purification, oysters had E. coli counts above the limit allowed by Brazilian legislation (≤ 230 MPN/100g). High TTC, and TC counts were observed in all the months of the study, and Salmonella spp. was present in August. All water samples were contaminated by TTC beyond the level allowed (> 43 MPN/100mL) in two months. The purification process of 36 h was effective in reducing the load of E. coli, TTC, and TC and eliminating Salmonella spp. in oysters.
Keywords
Oyster; Escherichia coli; purification process; sanitary control
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.