PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon
Version 1
: Received: 25 April 2023 / Approved: 2 May 2023 / Online: 2 May 2023 (07:37:53 CEST)
How to cite:
Mafo Kamga, L. D.; Founou, R. C.; Noubom, M.; Dimani, B. D.; Zemtsa, J. R.; Koudoum, P. L.; Mbossi, A. D.; Tchouangueu, T. F.; Noukeu, D.; Founou, L. L.; Gatsing, D. Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon. Preprints2023, 2023050068. https://doi.org/10.20944/preprints202305.0068.v1
Mafo Kamga, L. D.; Founou, R. C.; Noubom, M.; Dimani, B. D.; Zemtsa, J. R.; Koudoum, P. L.; Mbossi, A. D.; Tchouangueu, T. F.; Noukeu, D.; Founou, L. L.; Gatsing, D. Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon. Preprints 2023, 2023050068. https://doi.org/10.20944/preprints202305.0068.v1
Mafo Kamga, L. D.; Founou, R. C.; Noubom, M.; Dimani, B. D.; Zemtsa, J. R.; Koudoum, P. L.; Mbossi, A. D.; Tchouangueu, T. F.; Noukeu, D.; Founou, L. L.; Gatsing, D. Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon. Preprints2023, 2023050068. https://doi.org/10.20944/preprints202305.0068.v1
APA Style
Mafo Kamga, L. D., Founou, R. C., Noubom, M., Dimani, B. D., Zemtsa, J. R., Koudoum, P. L., Mbossi, A. D., Tchouangueu, T. F., Noukeu, D., Founou, L. L., & Gatsing, D. (2023). Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon. Preprints. https://doi.org/10.20944/preprints202305.0068.v1
Chicago/Turabian Style
Mafo Kamga, L. D., Luria Leslie Founou and Donatien Gatsing. 2023 "Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated From Children Under 5 Years With and Without Diarrhoea in Two Hospitals in Dschang, Cameroon" Preprints. https://doi.org/10.20944/preprints202305.0068.v1
Abstract
Extended-spectrum β-lactamase (ESBL) mediating resistance in Enterobacterales is a global public health issue, especially in low-and middle-income countries (LMICs) such as Cameroon. ESBL-producing Enterobacterales reduce therapeutic options and lead to the use of last resort drugs such as carbapenems even in vulnerable populations like children under five years. This study aims at determining the phenotypic and genotypic characteristics of ESBL-producing Escherichia coli (ESBL-Ec) isolated from children under five years with and without diarrhoea in two health care facilities in Dschang. A cross-sectional study was conducted from 3 February to 19 May 2022 in two hospitals in the city of Dschang, Cameroon.
Stool collected were cultured on Eosine Methylen Blue (EMB) medium. Enterosystem 18 R kit was used for bacterial identification. Evaluation of the resistance patterns and detection of ESBL production were performed with, the Kirby Bauer disk diffusion method and CHROMagar® ESBL medium, respectively. The genomic DNA of ESBL-Ec was extracted using the boiling method and subjected to conventional and multiplex PCRs for detection of blaSHV, blaCTX-M and blaTEM genes. Data were entered into ExcelTM 2016. Epi info and R software were used for statistical analyses with a p-value <0.05 considered statistically significant.
Out of the 125 children enrolled, 67.2% (84/125) were colonized by E. coli. Among these, 57.14% (48/84) were colonized by ESBL-Ec. ESBL- Ec isolates showed high levels of resistance to amoxicillin-clavulanic acid (96.22%), cefotaxime (75.47%), ceftriaxone (73.58%), ofloxacin (67.92%), levofloxacin (56.6%) and ciprofloxacin (54.71%). Children aged between 1-3 years (47.62%, p=0.09) and those who had taken an antibiotic one week prior to collection (70.83%, p=0.04) had a higher prevalence of ESBL-Ec with statistical significance. The majority of ESBL-Ec isolates (52.83%; 28/53) were co-producers of blaCTX-M and blaTEM. Infection prevention and control measures coupled with antimicrobial stewardship strategies need to be strengthened to reduce emergence and dissemination of ESBL-Ec among this vulnerable population.
Keywords
ESBLs; CTX-M; E. coli; Children; Cameroon
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.